UniProt UniProt http://www.uniprot.org Ebp1, an ErbB-3 binding protein, interacts with Rb and affects Rb transcriptional regulation. Ebp1, an ErbB-3 binding protein, interacts with Rb and affects Rb transcriptional regulation. J. Cell. Physiol. (0021-9541) 2001 Xia X., Cheng A., Lessor T., Zhang Y., Hamburger AW. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2B shows CoIp of FLAG-transfected PA2G4 with RB1(endogenous)in COS7 cells. Fig 2A human-au565 Homo sapiens AU565 adenocarcinoma au565 Human adenocarcinoma cell line AU565 http://www.atcc.org/documents/pdf/CellCatalog/Tools_Models.pdf anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Xia X., Cheng A., Lessor T., Zhang Y., Hamburger AW. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2B shows CoIp of FLAG-transfected PA2G4 with RB1(endogenous)in COS7 cells. Fig 2A Accepted 2006-JUN-22 by DAVET. J. Cell. Physiol. (0021-9541) 2001 Ebp1, an ErbB-3 binding protein, interacts with Rb and affects Rb transcriptional regulation. Ebp1, an ErbB-3 binding protein, interacts with Rb and affects Rb transcriptional regulation. J. Cell. Physiol. (0021-9541) 2001 Xia X., Cheng A., Lessor T., Zhang Y., Hamburger AW. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2B shows CoIp of FLAG-transfected PA2G4 with RB1(endogenous)in COS7 cells. Fig 2A in vitro In vitro pull down pull down antibody detection identification by antibody antibody detection Xia X., Cheng A., Lessor T., Zhang Y., Hamburger AW. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig 2B shows CoIp of FLAG-transfected PA2G4 with RB1(endogenous)in COS7 cells. Fig 2A Accepted 2006-JUN-22 by DAVET. J. Cell. Physiol. (0021-9541) 2001 pa2g4_human Proliferation-associated protein 2G4 PA2G4 Cell cycle protein p38-2G4 homolog ErbB3-binding protein 1 EBP1 protein protein human Homo sapiens Human MSGEDEQQEQTIAEDLVVTKYKMGGDIANRVLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKEMKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRKADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQKKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFTLRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEVQDAELKALLQSSASRKTQKKKKKKASKTAENATSGETLEENEAGD DIP protein Q9UQ80 original sequence version: 148 CD45466507AD6047 e2f1_human Transcription factor E2F1 Retinoblastoma-associated protein 1 Retinoblastoma-binding protein 3 PBR3 E2F1 RBBP3 pRB-binding protein E2F-1 protein protein human Homo sapiens Human MALAGAPAGGPCAPALEALLGAGALRLLDSSQIVIISAAQDASAPPAPTGPAAPAAGPCDPDLLLFATPQAPRPTPSAPRPALGRPPVKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEETVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF DIP protein Q01094 original sequence version: 173 DIP protein Q01094 original sequence version: 168 DIP protein Q01094 original sequence version: 149 DIP protein Q01094 original sequence version: 130 DIP protein Q01094 original sequence version: 125 DIP protein Q01094 original sequence version: 153 DIP protein Q01094 original sequence version: 180 003B3F654F0C60DF rb_human Retinoblastoma-associated protein pRb pp110 p105-Rb RB1 p110-RB1 protein protein human Homo sapiens Human MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK DIP protein P06400 original sequence version: 102 DIP protein P06400 original sequence version: 195 DIP protein P06400 original sequence version: 204 DIP protein P06400 original sequence version: 126 DIP protein P06400 original sequence version: 151 DIP protein P06400 original sequence version: 153 DIP protein P06400 original sequence version: 181 DIP protein P06400 original sequence version: 184 DIP protein P06400 original sequence version: 213 DIP protein P06400 original sequence version: 208 DIP protein P06400 original sequence version: 174 DIP protein P06400 original sequence version: 212 DIP protein P06400 original sequence version: 179 DIP protein P06400 original sequence version: 146 C8E746111E19CC32 rb1-pa2g4-1 1 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association pa2g4-rb1-1 2 3 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-925198]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 395-395, sequence length 394 [EBI-925198]322-395 ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role prey prey chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association 3a and 4B rb1-pa2g4-2 2 3 unspecified role unspecified role prey prey human-au565 Homo sapiens AU565 adenocarcinoma au565 Human adenocarcinoma cell line AU565 http://www.atcc.org/documents/pdf/CellCatalog/Tools_Models.pdf 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined range binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) physical association physical association Dephosphorylation of PA2G4 increases the interaction. AU565 cell lysates treated with or without alakaline phosphatase have been used. 5 pa2g4-rb1-2 2 3 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role prey prey human-au565 Homo sapiens AU565 adenocarcinoma au565 Human adenocarcinoma cell line AU565 http://www.atcc.org/documents/pdf/CellCatalog/Tools_Models.pdf physical association physical association 7 Interaction was largely enhanced by overexpressing RB1. pa2g4-e2f1-1 2 3 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) 4 unspecified role unspecified role prey prey human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells physical association physical association 7 Interaction was enhanced when RB1 was overexpressed im MCF-7 cells.