IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1. The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 Hersh D., Monack DM., Smith MR., Ghori N., Falkow S., Zychlinsky A. Apoptosis - Interactions involving proteins with a function related to apoptosis mouse-j774a-1 Mus musculus macrophage j774a-1 mouse-macrophage coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip monoclonal western monoclonal antibody western blot monoclonal western Hersh D., Monack DM., Smith MR., Ghori N., Falkow S., Zychlinsky A. Apoptosis - Interactions involving proteins with a function related to apoptosis Salmonella typhi protein SipB is expressed in the Shigella flexneri strain M90T defective of its homolgue virulence gene (Ipa-B). This shigella strain recovers virulence after the transfection of SipB. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1. The Salmonella invasin SipB induces macrophage apoptosis by binding to caspase-1. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 Hersh D., Monack DM., Smith MR., Ghori N., Falkow S., Zychlinsky A. Apoptosis - Interactions involving proteins with a function related to apoptosis in vitro In vitro pull down pull down polyclonal western polyclonal antibody western blot polyclonal western Hersh D., Monack DM., Smith MR., Ghori N., Falkow S., Zychlinsky A. Apoptosis - Interactions involving proteins with a function related to apoptosis Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1999 casp1_mouse Caspase-1 Interleukin-1 beta convertase Interleukin-1 beta-converting enzyme p45 Casp1 Il1bc protein protein mouse Mus musculus Mouse MADKILRAKRKQFINSVSIGTINGLLDELLEKRVLNQEEMDKIKLANITAMDKARDLCDHVSKKGPQASQIFITYICNEDCYLAGILELQSAPSAETFVATEDSKGGHPSSSETKEEQNKEDGTFPGLTGTLKFCPLEKAQKLWKENPSEIYPIMNTTTRTRLALIICNTEFQHLSPRVGAQVDLREMKLLLEDLGYTVKVKENLTALEMVKEVKEFAACPEHKTSDSTFLVFMSHGIQEGICGTTYSNEVSDILKVDTIFQMMNTLKCPSLKDKPKVIIIQACRGEKQGVVLLKDSVRDSEEDFLTDAIFEDDGIKKAHIEKDFIAFCSSTPDNVSWRHPVRGSLFIESLIKHMKEYAWSCDLEDIFRKVRFSFEQPEFRLQMPTADRVTLTKRFYLFPGH DIP protein P29452 original sequence version: 87 DIP protein P29452 original sequence version: 158 DIP protein P29452 original sequence version: 123 DIP protein P29452 original sequence version: 122 3BEBCEFA67C69B03 sipb_salti Cell invasion protein SipB Effector protein SipB STY3008 t2787 sipB protein protein salti Salmonella typhi MVNDASSISRSGYTQNPRLAEAAFEGVRKNTDFLKAADKAFKDVVATKAGDLKAGTKSGESAINTVGLKPPTDAAREKLSSEGQLTLLLGKLMTLLGDVSLSQLESRLAVWQAMIESQKEMGIQVSKEFQTALGEAQEATDLYEASIKKTDTAKSVYDAAAKKLTQAQNKLQSLDPADPGYAQAEAAVEQAGKEATEAKEALDKATDATVKAGTDAKAKAEKADNILTKFQGTANAASQNQVSQGEQDNLSNVARLTMLMAMFIEIVGKNTEESLQNDLALFNALQEGRQAEMEKKSAEFQEETRKAEETNRIMGCIGKVLGALLTIVSVVAAVFTGGASLALAAVGLAVMVADEIVKAATGVSFIQQALNPIMEHVLKPLMELIGKAITKALEGLGVDKKTAEMAGSIVGAIVAAIAMVAVIVVVAVVGKGAAAKLGNALSKMMGETIKKLVPNVLKQLAQNGSKLFTQGMQRITSGLGNVGSKMGLQTNALSKELVGNTLNKVALGMEVTNTAAQSAGGVAEGVFIKNASEALADFMLARFAMDQIQQWLKQSVEIFGENQKVTAELQKAMSSAVQQNADASRFILRQSRA 8570D64343276639 sipb-casp1-1 2 3 unspecified role unspecified role prey prey mouse-j774a-1 Mus musculus macrophage j774a-1 mouse-macrophage 4 unspecified role unspecified role bait bait gst-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined shifl Shigella flexneri physical association physical association sipb-casp1-2 1 4 unspecified role unspecified role bait bait n-term flag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range shifl Shigella flexneri 3 unspecified role unspecified role prey prey mouse-j774a-1 Mus musculus macrophage j774a-1 mouse-macrophage physical association physical association The co-ip is carried out after infection of mouse macrophage j77a4.1 by the shigella strain (defective of sipb homologue IpaB) expressing salmonella sipb.