IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mpl traffics to the cell surface through conventional and unconventional routes. Mpl traffics to the cell surface through conventional and unconventional routes. Traffic (1398-9219) 2014 imex curation Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Only protein-protein interactions human-hel Homo sapiens HEL erythroleukemia cell line hel human erythroleukemia established from the peripheral blood of a 30-year-old man with erythroleukemia (AML M6) in relapse (after treatment for Hodgkin's leukemia) pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Accepted 2015-FEB-25 by ORCHARD Traffic (1398-9219) 2014 Only protein-protein interactions imex curation Mpl traffics to the cell surface through conventional and unconventional routes. Mpl traffics to the cell surface through conventional and unconventional routes. Traffic (1398-9219) 2014 imex curation Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Only protein-protein interactions human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Accepted 2015-FEB-25 by ORCHARD Traffic (1398-9219) 2014 Only protein-protein interactions imex curation Mpl traffics to the cell surface through conventional and unconventional routes. Mpl traffics to the cell surface through conventional and unconventional routes. Traffic (1398-9219) 2014 imex curation Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Only protein-protein interactions human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Accepted 2015-FEB-25 by ORCHARD Traffic (1398-9219) 2014 Only protein-protein interactions imex curation Mpl traffics to the cell surface through conventional and unconventional routes. Mpl traffics to the cell surface through conventional and unconventional routes. Traffic (1398-9219) 2014 imex curation Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Only protein-protein interactions human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Accepted 2015-FEB-25 by ORCHARD Traffic (1398-9219) 2014 Only protein-protein interactions imex curation Mpl traffics to the cell surface through conventional and unconventional routes. Mpl traffics to the cell surface through conventional and unconventional routes. Traffic (1398-9219) 2014 imex curation Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Only protein-protein interactions human-hel Homo sapiens HEL erythroleukemia cell line hel human erythroleukemia established from the peripheral blood of a 30-year-old man with erythroleukemia (AML M6) in relapse (after treatment for Hodgkin's leukemia) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Accepted 2015-FEB-25 by ORCHARD Traffic (1398-9219) 2014 Only protein-protein interactions imex curation Mpl traffics to the cell surface through conventional and unconventional routes. Mpl traffics to the cell surface through conventional and unconventional routes. Traffic (1398-9219) 2014 imex curation Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Only protein-protein interactions human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cleyrat C., Darehshouri A., Steinkamp M.P., Vilaine M., Boassa D., Ellisman M.H., Hermouet S., Wilson B.S. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that all transfectants are of Human origin. bwilson@salud.unm.edu Accepted 2015-FEB-25 by ORCHARD Traffic (1398-9219) 2014 Only protein-protein interactions imex curation tpor_human Thrombopoietin receptor MPL TPOR Myeloproliferative leukemia protein Proto-oncogene c-Mpl protein protein human Homo sapiens Human MPSWALFMVTSCLLLAPQNLAQVSSQDVSLLASDSEPLKCFSRTFEDLTCFWDEEEAAPSGTYQLLYAYPREKPRACPLSSQSMPHFGTRYVCQFPDQEEVRLFFPLHLWVKNVFLNQTRTQRVLFVDSVGLPAPPSIIKAMGGSQPGELQISWEEPAPEISDFLRYELRYGPRDPKNSTGPTVIQLIATETCCPALQRPHSASALDQSPCAQPTMPWQDGPKQTSPSREASALTAEGGSCLISGLQPGNSYWLQLRSEPDGISLGGSWGSWSLPVTVDLPGDAVALGLQCFTLDLKNVTCQWQQQDHASSQGFFYHSRARCCPRDRYPIWENCEEEEKTNPGLQTPQFSRCHFKSRNDSIIHILVEVTTAPGTVHSYLGSPFWIHQAVRLPTPNLHWREISSGHLELEWQHPSSWAAQETCYQLRYTGEGHQDWKVLEPPLGARGGTLELRPRSRYRLQLRARLNGPTYQGPWSSWSDPTRVETATETAWISLVTALHLVLGLSAVLGLLLLRWQFPAHYRRLRHALWPSLPDLHRVLGQYLRDTAALSPPKATVSDTCEEVEPSLLEILPKSSERTPLPLCSSQAQMDYRRLQPSCLGTMPLSVCPPMAESGSCCTTHIANHSYLPLSYWQQP D25D8D8959359DDC jak2_human Tyrosine-protein kinase JAK2 Janus kinase 2 JAK2 protein protein human Homo sapiens Human MGMACLTMTEMEGTSTSSIYQNGDISGNANSMKQIDPVLQVYLYHSLGKSEADYLTFPSGEYVAEEICIAASKACGITPVYHNMFALMSETERIWYPPNHVFHIDESTRHNVLYRIRFYFPRWYCSGSNRAYRHGISRGAEAPLLDDFVMSYLFAQWRHDFVHGWIKVPVTHETQEECLGMAVLDMMRIAKENDQTPLAIYNSISYKTFLPKCIRAKIQDYHILTRKRIRYRFRRFIQQFSQCKATARNLKLKYLINLETLQSAFYTEKFEVKEPGSGPSGEEIFATIIITGNGGIQWSRGKHKESETLTEQDLQLYCDFPNIIDVSIKQANQEGSNESRVVTIHKQDGKNLEIELSSLREALSFVSLIDGYYRLTADAHHYLCKEVAPPAVLENIQSNCHGPISMDFAISKLKKAGNQTGLYVLRCSPKDFNKYFLTFAVERENVIEYKHCLITKNENEEYNLSGTKKNFSSLKDLLNCYQMETVRSDNIIFQFTKCCPPKPKDKSNLLVFRTNGVSDVPTSPTLQRPTHMNQMVFHKIRNEDLIFNESLGQGTFTKIFKGVRREVGDYGQLHETEVLLKVLDKAHRNYSESFFEAASMMSKLSHKHLVLNYGVCVCGDENILVQEFVKFGSLDTYLKKNKNCINILWKLEVAKQLAWAMHFLEENTLIHGNVCAKNILLIREEDRKTGNPPFIKLSDPGISITVLPKDILQERIPWVPPECIENPKNLNLATDKWSFGTTLWEICSGGDKPLSALDSQRKLQFYEDRHQLPAPKWAELANLINNCMDYEPDFRPSFRAIIRDLNSLFTPDYELLTENDMLPNMRIGALGFSGAFEDRDPTQFEERHLKFLQQLGKGNFGSVEMCRYDPLQDNTGEVVAVKKLQHSTEEHLRDFEREIEILKSLQHDNIVKYKGVCYSAGRRNLKLIMEYLPYGSLRDYLQKHKERIDHIKLLQYTSQICKGMEYLGTKRYIHRDLATRNILVENENRVKIGDFGLTKVLPQDKEYYKVKEPGESPIFWYAPESLTESKFSVASDVWSFGVVLYELFTYIEKSKSPPAEFMRMIGNDKQGQMIVFHLIELLKNNGRLPRPDGCPDEIYMIMTECWNNNVNQRPSFRDLALRVDQIRDNMAG DIP protein O60674 original sequence version: 119 DIP protein O60674 original sequence version: 126 DIP protein O60674 original sequence version: 137 DIP protein O60674 original sequence version: 129 C30669EF1A7DA80C mpl-jak2 5 8 unspecified role unspecified role prey prey O60674:p.Val617Phe mutation mutation certain certain sequence position certain certain certain sequence position certain V F Wed May 02 14:29:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val617phe' Tue Jun 30 17:53:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val617Phe' 7 unspecified role unspecified role bait bait physical association physical association 1F mpl-jak2-1 6 8 unspecified role unspecified role prey prey mk02_kusabira_orange fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait physical association physical association 1F jak2-mpl-1 3 8 unspecified role unspecified role neutral component neutral component 7 tag fluorescence tag visualisation by fluorescence tag fluorescence immunocytochemistry immunocytochemistry unspecified role unspecified role neutral component neutral component m_orange fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 2D jak2-mpl-2 4 8 unspecified role unspecified role neutral component neutral component m_citrine fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O60674:p.Val617Phe mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V F Wed May 02 16:12:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val617phe' Tue Jun 30 18:50:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val617Phe' 7 unspecified role unspecified role neutral component neutral component m_orange fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined true colocalization colocalization 2 F/G jak2-mpl-3 1 8 unspecified role unspecified role neutral component neutral component O60674:p.Val617Phe mutation mutation certain certain sequence position certain certain certain sequence position certain true V F Wed May 02 18:41:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val617phe' Tue Jun 30 16:00:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val617Phe' 7 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 3 C/D association was low jak2-mpl-4 2 7 unspecified role unspecified role neutral component neutral component mk02_kusabira_orange fluorescent prot tag fluorescent protein tag fluorescent prot tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 3 E/F association was low