IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Synaptic Protein α1-Takusan Mitigates Amyloid-β-Induced Synaptic Loss via Interaction with Tau and Postsynaptic Density-95 at Postsynaptic Sites. Synaptic Protein α1-Takusan Mitigates Amyloid-β-Induced Synaptic Loss via Interaction with Tau and Postsynaptic Density-95 at Postsynaptic Sites. J. Neurosci. (0270-6474) 2013 imex curation Nakanishi N., Ryan S.D., Zhang X., Khan A., Holland T., Cho E.G., Huang X., Liao F.F., Xu H., Lipton S.A., Tu S. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Affinomics - Interactions curated for the Affinomics consortium. Alzheimers - Interactions investigated in the context of Alzheimers disease Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that in the construct "mcherry-DSP-95", DSP-95 is of Murine origin. Figure 11 and 12 have not been annotated as they use artificial chimeric proteins. nnakanishi@sanfordburnham.org,shichuntu@sanfordburnham.org 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions rat-cortical_neuron Rat cortical Neurons brain-cortex_neuron Cerebral cortical neuron anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Nakanishi N., Ryan S.D., Zhang X., Khan A., Holland T., Cho E.G., Huang X., Liao F.F., Xu H., Lipton S.A., Tu S. Affinomics - Interactions curated for the Affinomics consortium. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that in the construct "mcherry-DSP-95", DSP-95 is of Murine origin. Figure 11 and 12 have not been annotated as they use artificial chimeric proteins. nnakanishi@sanfordburnham.org,shichuntu@sanfordburnham.org Accepted 2013-OCT-01 by PPORRAS 14-Oct-2013: Contacted by IntAct-Help. J. Neurosci. (0270-6474) 2013 Only protein-protein interactions imex curation I have removed the Expt Synaptic Protein α1-Takusan Mitigates Amyloid-β-Induced Synaptic Loss via Interaction with Tau and Postsynaptic Density-95 at Postsynaptic Sites. Synaptic Protein α1-Takusan Mitigates Amyloid-β-Induced Synaptic Loss via Interaction with Tau and Postsynaptic Density-95 at Postsynaptic Sites. J. Neurosci. (0270-6474) 2013 imex curation Nakanishi N., Ryan S.D., Zhang X., Khan A., Holland T., Cho E.G., Huang X., Liao F.F., Xu H., Lipton S.A., Tu S. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Affinomics - Interactions curated for the Affinomics consortium. Alzheimers - Interactions investigated in the context of Alzheimers disease Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that in the construct "mcherry-DSP-95", DSP-95 is of Murine origin. Figure 11 and 12 have not been annotated as they use artificial chimeric proteins. nnakanishi@sanfordburnham.org,shichuntu@sanfordburnham.org 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Nakanishi N., Ryan S.D., Zhang X., Khan A., Holland T., Cho E.G., Huang X., Liao F.F., Xu H., Lipton S.A., Tu S. Affinomics - Interactions curated for the Affinomics consortium. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that in the construct "mcherry-DSP-95", DSP-95 is of Murine origin. Figure 11 and 12 have not been annotated as they use artificial chimeric proteins. nnakanishi@sanfordburnham.org,shichuntu@sanfordburnham.org Accepted 2013-OCT-01 by PPORRAS 14-Oct-2013: Contacted by IntAct-Help. J. Neurosci. (0270-6474) 2013 Only protein-protein interactions imex curation I have removed the Expt Synaptic Protein α1-Takusan Mitigates Amyloid-β-Induced Synaptic Loss via Interaction with Tau and Postsynaptic Density-95 at Postsynaptic Sites. Synaptic Protein α1-Takusan Mitigates Amyloid-β-Induced Synaptic Loss via Interaction with Tau and Postsynaptic Density-95 at Postsynaptic Sites. J. Neurosci. (0270-6474) 2013 imex curation Nakanishi N., Ryan S.D., Zhang X., Khan A., Holland T., Cho E.G., Huang X., Liao F.F., Xu H., Lipton S.A., Tu S. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Affinomics - Interactions curated for the Affinomics consortium. Alzheimers - Interactions investigated in the context of Alzheimers disease Parkinsons - Interactions investigated in the context of Parkinsons disease The authors confirm that in the construct "mcherry-DSP-95", DSP-95 is of Murine origin. Figure 11 and 12 have not been annotated as they use artificial chimeric proteins. nnakanishi@sanfordburnham.org,shichuntu@sanfordburnham.org 14-Oct-2013: Contacted by IntAct-Help. Only protein-protein interactions rat-cortical_neuron Rat cortical Neurons brain-cortex_neuron Cerebral cortical neuron pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Nakanishi N., Ryan S.D., Zhang X., Khan A., Holland T., Cho E.G., Huang X., Liao F.F., Xu H., Lipton S.A., Tu S. Affinomics - Interactions curated for the Affinomics consortium. Affinomics - Interactions curated for the Affinomics consortium. The authors confirm that in the construct "mcherry-DSP-95", DSP-95 is of Murine origin. Figure 11 and 12 have not been annotated as they use artificial chimeric proteins. nnakanishi@sanfordburnham.org,shichuntu@sanfordburnham.org Accepted 2013-OCT-01 by PPORRAS 14-Oct-2013: Contacted by IntAct-Help. J. Neurosci. (0270-6474) 2013 Only protein-protein interactions imex curation I have removed the Expt a6nar8_mouse protein protein mouse Mus musculus Mouse MTKKRSKINELEELKLDMRKISNDMEEMCGILNLYMYEDLNYRMNTEFNIIKSQHEKTMLDMNKMIQSIIGSMQYSKELIEDNYSYSIKEDHLLRECTQLNENVRILLNENRRLLVEQAGHKCPVGKKRGSLRRPARTSVSQVPRNSSVI CE9F84DBEF8DFFBB tau_rat Microtubule-associated protein tau Mapt Mtapt Tau Neurofibrillary tangle protein Paired helical filament-tau protein protein rat Rattus norvegicus (Rat) Rat MAEPRQEFDTMEDQAGDYTMLQDQEGDMDHGLKESPPQPPADDGSEEPGSETSDAKSTPTAEDVTAPLVEERAPDKQATAQSHTEIPEGTTAEEAGIGDTPNMEDQAAGHVTQEPQKVEIFSQSLLVEPGRREGQAPDSGISDWTHQQVPSMSGAPLPPQGLREATHQPLGTRPEDVERSHPASELLWQESPQKEAWGKDRLGSEEEVDEDITMDESSQESPPSQASLAPGTATPQARSVSASGVSGETTSIPGFPAEGSIPLPADFFSKVSAETQASPPEGPGTGPSEEGHEAAPEFTFHVEIKASAPKEQDLEGATVVGAPAEEQKARGPSVGKGTKEASLLEPTDKQPAAGLPGRPVSRVPQLKARVAGVSKDRTGNDEKKAKTSTPSCAKTPSNRPCLSPTRPTPGSSDPLIKPSSPAVCPEPATSPKYVSSVTPRNGSPGTKQMKLKGADGKTGAKIATPRGAATPGQKGTSNATRIPAKTTPSPKTPPGSGEPPKSGERSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSASKSRLQTAPVPMPDLKNVRSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVHIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL DIP protein P19332 original sequence version: 107 mint Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization 915DCA04EF0B2902 ensmusg00000079-mapt 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 7B ensmusg000000-mapt-1 3 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range proximity proximity close-contact colocalization neighbourhood interaction 7C ensmusg000000-mapt-1 2 4 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range d2_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 9E