IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Native STIM2 and ORAI1 proteins form a calcium-sensitive and thapsigargin-insensitive complex in cortical neurons. Native STIM2 and ORAI1 proteins form a calcium-sensitive and thapsigargin-insensitive complex in cortical neurons. J. Neurochem. (0022-3042) 2013 imex curation Gruszczynska-Biegala J., Kuznicki J. Affinomics - Interactions curated for the Affinomics consortium. jkuznicki@iimcb.gov.pl 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions rat-cortical_neuron Rat cortical Neurons brain-cortex_neuron Cerebral cortical neuron anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gruszczynska-Biegala J., Kuznicki J. Affinomics - Interactions curated for the Affinomics consortium. jkuznicki@iimcb.gov.pl Accepted 2014-SEP-18 by ORCHARD J. Neurochem. (0022-3042) 2013 Only protein-protein interactions imex curation Native STIM2 and ORAI1 proteins form a calcium-sensitive and thapsigargin-insensitive complex in cortical neurons. Native STIM2 and ORAI1 proteins form a calcium-sensitive and thapsigargin-insensitive complex in cortical neurons. J. Neurochem. (0022-3042) 2013 imex curation Gruszczynska-Biegala J., Kuznicki J. Affinomics - Interactions curated for the Affinomics consortium. jkuznicki@iimcb.gov.pl 20-Oct-2014: Contacted by IntAct-Help. Only protein-protein interactions rat-cortical_neuron Rat cortical Neurons brain-cortex_neuron Cerebral cortical neuron pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Gruszczynska-Biegala J., Kuznicki J. Affinomics - Interactions curated for the Affinomics consortium. jkuznicki@iimcb.gov.pl Accepted 2014-SEP-18 by ORCHARD J. Neurochem. (0022-3042) 2013 Only protein-protein interactions imex curation crcm1_rat Calcium release-activated calcium channel protein 1 Orai1 Cracm1 Tmem142a Protein orai-1 Transmembrane protein 142A protein protein rat Rattus norvegicus (Rat) Rat MHPEPAPPPNNSNPELPLSGGSSTSGSRRSRRRSGDGEPTGAPPLPPPPAVSYPDWIGQSYSEVMSLNEHSMQALSWRKLYLSRAKLKASSRTSALLSGFAMVAMVEVQLDTDHDYPPGLLIVFSACTTVLVAVHLFALMISTCILPNIEAVSNVHNLNSVKESPHERMHRHIELAWAFSTVIGTLLFLAEVVLLCWVKFLPLKRQAGQPSPTKPPTEPAVVVANSSNNGGITPGEAAAIASTAIMVPCGLVFIVFAVHFYRSLVSHKTDRQFQELNELAEFARLQDQLDHRGDHSLTPGTHYA 98264D8880982C74 d4a5x1_rat Stim2 protein protein rat Rattus norvegicus (Rat) Rat MLLFGLLVAGVADGCDLVPRHLRGRRASGSAGAAASPSAAAAGERQALLTDPCMSLSPPCFTEEDRFSLEALQTIHKQMDDDKDGGIEVDESDEFIREDMKYKDATNKHSHLHREDKHITVEDLWKQWKTSEVHNWTLEDTLQWLIEFVELPQYEKNFRDNNVKGTTLPRIAVHETSFMISQLKISDRSHRQKLQLKALDVVLFGPLTRPPHNWMKDFILTISIVIGVGGCWFAYTQNKTSKEHVAKMMKDLESLQTAEQSLMDLQERLEKAQEENRTVAVEKQNLERKMMDEINYAKEEACRLRELREGAECELSRRQYAEQELEQVRMALKKAEKEFELRSSWSVPDALQKWLQLTHEVEVQYYNIKRQNAEMQLAIAKDEAEKIKKKRSTVFGTLHVAHSSSLDEVDHKILEAKKALSELTTCLRERLFRWQQIEKICGFQIAHNSGLPSLTSSLYSDHSWVVMPRVSIPPYPIAGGVDDLDEDTPPIVSQFPGTVAKPAGSLARSSSLCRSRRSIVPSSPQSQRAQLPAHAPLAAHPRHPHHPQHSLPSPDPDILSVSSCPALYRNEEEEEAIYFTAEKQWEVPDTASECDSLNSSIGRKQSPPSSLEIYQTLSSRKISRDELSLEDSSRGDSPVTADVSRGSPECVGLTETKSMIFSPASRVYNGILEKSCSMHQLSSGIPVPNPRHTSCSLAGSDSKPVQEASSVSRVGSIPHDLCHNGEKSKKPSKIKSLFKKKSK F726A28D2FD9A54F stim1_rat Stromal interaction molecule 1 Stim1 Sim protein protein rat Rattus norvegicus (Rat) Rat MDVCARLALWLLWGLLLHQGQSLSHSHSEKNTGASSGATSEESTEAEFCRIDKPLCHSEDEKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDLWKAWKASEVYNWTVDEVIQWLITYVELPQYEETFRKLQLTGHAMPRLAVTNTTMTGTVLKMTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEHMKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRLKELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQYYNIKKQNAERQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVTAALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDMDEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHTSDRQRVAPKPPQMGRAADEALNATSSNGSHRLIEGVHPGSLVEKLPDSPALAKKTILALNHGLDKAHSLMELNPSVPPGGSPLLDSSHSHSPSSPDPDTPSPVGDSRALQGSRNTRIPHLAGKKAMAEEDNGSIGEETDSSPGRKKFPLKIFKKPLKK FD2DA28427D2E41E stim2-orai1 1 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association 1 EGTA (removes calcium ions) orai1-stim2 1 3 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 1 EGTA (removes calcium ions) orai1-stim2-2 2 4 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 2,3,4,5, Supp fig 1 EGTA containing medium (chelates/removes calcium ions). high-calcium ion containing medium Results suggest that STIM2-ORAI1 complexes formed as a result of decreased cytosolic calcium levels. BAPTA-AM - a calcium chelator that penetrates live cells and decreases intracellular calcium concentrations Low extracellular calcium levels. orai1-stim2-3 2 3 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 6 Thapsigargin (a non-competitive inhibitor of sarco/endoplasmic reticulum Ca2+ ATPases resulting in increased cytosolic (intracellular) calcium concentration. The addition of the SOCE (store-operated calcium entry) inhibitor ML9 (1-(5-Chloronaphthalenesulfonyl)homopiperazine hydrochloride) after addition of Thapsigargin decreased the number of complexes nearly to the state before thapsigargin stimulation.