IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. Clin. Cancer Res. (1078-0432) 2013 imex curation Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer Affinomics - Interactions curated for the Affinomics consortium. I have not looked at all the Supplementary images as they take so long to download - but ones I have looked at are not relevant and neither do legends. Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-u87 Human U-87 MG glioblastoma cell line u87 pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer I have not looked at all the Supplementary images as they take so long to download - but ones I have looked at are not relevant and neither do legends. Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu Accepted 2014-AUG-12 by ORCHARD Clin. Cancer Res. (1078-0432) 2013 Only protein-protein interactions imex curation Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. Clin. Cancer Res. (1078-0432) 2013 imex curation Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer Affinomics - Interactions curated for the Affinomics consortium. I have not looked at all the Supplementary images as they take so long to download - but ones I have looked at are not relevant and neither do legends. Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-glioblastoma Homo sapiens glioblastoma glioblastoma Glioblastoma Glioblastoma is the same as 'Glioblastoma multiforme' (GBM) -is the same as Astrocytoma Grade 4. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer I have not looked at all the Supplementary images as they take so long to download - but ones I have looked at are not relevant and neither do legends. Lysed GBM83 sphere cells:- patient-derived glioblastoma cells grown as spheres that are enriched with Glioma Stem Cells. Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu Accepted 2014-AUG-12 by ORCHARD Clin. Cancer Res. (1078-0432) 2013 Only protein-protein interactions imex curation Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. Clin. Cancer Res. (1078-0432) 2013 imex curation Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer Affinomics - Interactions curated for the Affinomics consortium. I have not looked at all the Supplementary images as they take so long to download - but ones I have looked at are not relevant and neither do legends. Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer I have not looked at all the Supplementary images as they take so long to download - but ones I have looked at are not relevant and neither do legends. Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu Accepted 2014-AUG-12 by ORCHARD Clin. Cancer Res. (1078-0432) 2013 Only protein-protein interactions imex curation Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. Clin. Cancer Res. (1078-0432) 2013 imex curation Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer Affinomics - Interactions curated for the Affinomics consortium. I have not looked at all the Supplementary images as they take so long to download - but ones I have looked at are not relevant and neither do legends. Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer I have not looked at all the Supplementary images as they take so long to download - but ones I have looked at are not relevant and neither do legends. Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu Accepted 2014-AUG-12 by ORCHARD Clin. Cancer Res. (1078-0432) 2013 Only protein-protein interactions imex curation Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. Impairment of glioma stem cell survival and growth by a novel inhibitor for Survivin-Ran protein complex. Clin. Cancer Res. (1078-0432) 2013 imex curation Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer Affinomics - Interactions curated for the Affinomics consortium. I have not looked at all the Supplementary images as they take so long to download - but ones I have looked at are not relevant and neither do legends. Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Guvenc H., Pavlyukov M.S., Joshi K., Kurt H., Banasavadi-Siddegowda Y.K., Mao P., Hong C., Yamada R., Kwon C.H., Bhasin D., Chettiar S., Kitange G., Park I.H., Sarkaria J.N., Li C., Shakhparonov M.I., Nakano I. Cancer - Interactions investigated in the context of cancer I have not looked at all the Supplementary images as they take so long to download - but ones I have looked at are not relevant and neither do legends. Survivin has been entered as Human based on References 26, 27 (PDB entry), 31 (Blat of primers used), 32 and 33. SMAC/DIABLO has been entered as Human based on reference 31 (Blat of primers). [The stated reference (30) - Pistollato F et al., for "His-tagged Smac/DAIBLO, Survivin WT and Survivin F101A/L102A were expressed and purified as described previously." does not mention any of these molecules. ]. Ichiro.Nakano@osumc.edu Accepted 2014-AUG-12 by ORCHARD Clin. Cancer Res. (1078-0432) 2013 Only protein-protein interactions imex curation ran_human GTP-binding nuclear protein Ran GTPase Ran Ras-related nuclear protein Ras-like protein TC4 Androgen receptor-associated protein 24 RAN ARA24 OK/SW-cl.81 protein protein human Homo sapiens Human MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL DIP protein P62826 original sequence version: 138 DIP protein P62826 original sequence version: 67 DIP protein P62826 original sequence version: 93 DIP protein P62826 original sequence version: 125 DIP protein P62826 original sequence version: 96 D5C9B7275C34BCE0 llp-3 LLP-3 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) birc5_human Baculoviral IAP repeat-containing protein 5 Apoptosis inhibitor survivin Apoptosis inhibitor 4 BIRC5 API4 IAP4 protein protein human Homo sapiens Human MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD DIP protein O15392 original sequence version: 129 DIP protein O15392 original sequence version: 132 DIP protein O15392-1 original sequence version: 141 DIP protein O15392-1 original sequence version: 143 9E7CADCDF2822286 dbloh_human Diablo homolog, mitochondrial Second mitochondria-derived activator of caspase Direct IAP-binding protein with low pI DIABLO SMAC protein protein human Homo sapiens Human MAALKSWLSRSVTSFFRYRQCLCVPVVANFKKRCFSELIRPWHKTVTIGFGVTLCAVPIAQKSEPHSLSSEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESEQEAYLRED 70C2AE0DC654D031 xiap_human E3 ubiquitin-protein ligase XIAP Inhibitor of apoptosis protein 3 X-linked inhibitor of apoptosis protein IAP-like protein XIAP API3 IAP3 BIRC4 Baculoviral IAP repeat-containing protein 4 RING-type E3 ubiquitin transferase XIAP protein protein human Homo sapiens Human MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS DIP protein P98170 original sequence version: 168 DIP protein P98170 original sequence version: 118 DIP protein P98170 original sequence version: 153 9D394C16D45EB635 birc5-llp_3 3 8 unspecified role unspecified role neutral component neutral component O15392:p.[Phe101Ala;Leu102Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain F A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 18:36:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe101ala,leu102ala' Tue Jun 30 20:13:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Phe101Ala;Leu102Ala]' ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3C diablo-birc5-5 5 8 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 4A-1 birc5-diablo 5 7 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 4A-3 diablo-birc5-4 5 9 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 4A-1 diablo-birc5-3 5 10 unspecified role unspecified role prey prey bir2_and3_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 4A-2 birc5-ran 5 8 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 4A-3i birc5-ran-1 5 8 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 4A-5 birc5-ran-2 4 8 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 4B birc5-ran-3 4 6 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 4B birc5-llp_3-1 1 8 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component 7 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) proximity proximity close-contact colocalization neighbourhood interaction 4C birc5-ran-6 1 6 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 4C birc5-ran-5 2 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 5A