IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Elevated MARK2-dependent phosphorylation of Tau in Alzheimer's disease. Elevated MARK2-dependent phosphorylation of Tau in Alzheimer's disease. J. Alzheimers Dis. (1387-2877) 2013 imex curation Gu G.J., Wu D., Lund H., Sunnemark D., Kvist A.J., Milner R., Eckersley S., Nilsson L.N., Agerman K., Landegren U., Kamali-Moghaddam M. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease Affinomics - Interactions curated for the Affinomics consortium. rhTau 3T3 cells with stable expression of recombinant human "4R" tau protein. TAU has been entered as isoform "8" -P10636-8 (also known as Tau-4, Tau 441 and Tau-F) as this is the only isoform listed in UniProt with a serine at positions 262 and 356. This is a "4R" isoform, has four (4R) ~32 amino acid imperfect repeats in the microtubule binding domain. [The rabbit polyclonal antibody against phosphorylated Ser262 of tau ( AGG5759), AstraZeneca) cannot be found.] Masood.Kamali@igp.uu.se. 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Gu G.J., Wu D., Lund H., Sunnemark D., Kvist A.J., Milner R., Eckersley S., Nilsson L.N., Agerman K., Landegren U., Kamali-Moghaddam M. Alzheimers - Interactions investigated in the context of Alzheimers disease rhTau 3T3 cells with stable expression of recombinant human "4R" tau protein. TAU has been entered as isoform "8" -P10636-8 (also known as Tau-4, Tau 441 and Tau-F) as this is the only isoform listed in UniProt with a serine at positions 262 and 356. This is a "4R" isoform, has four (4R) ~32 amino acid imperfect repeats in the microtubule binding domain. [The rabbit polyclonal antibody against phosphorylated Ser262 of tau ( AGG5759), AstraZeneca) cannot be found.] Masood.Kamali@igp.uu.se. Accepted 2013-AUG-07 by ORCHARD 09-Aug-2013: Contacted by IntAct-Help. J. Alzheimers Dis. (1387-2877) 2013 Only protein-protein interactions imex curation Elevated MARK2-dependent phosphorylation of Tau in Alzheimer's disease. Elevated MARK2-dependent phosphorylation of Tau in Alzheimer's disease. J. Alzheimers Dis. (1387-2877) 2013 imex curation Gu G.J., Wu D., Lund H., Sunnemark D., Kvist A.J., Milner R., Eckersley S., Nilsson L.N., Agerman K., Landegren U., Kamali-Moghaddam M. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Alzheimers - Interactions investigated in the context of Alzheimers disease Affinomics - Interactions curated for the Affinomics consortium. rhTau 3T3 cells with stable expression of recombinant human "4R" tau protein. TAU has been entered as isoform "8" -P10636-8 (also known as Tau-4, Tau 441 and Tau-F) as this is the only isoform listed in UniProt with a serine at positions 262 and 356. This is a "4R" isoform, has four (4R) ~32 amino acid imperfect repeats in the microtubule binding domain. [The rabbit polyclonal antibody against phosphorylated Ser262 of tau ( AGG5759), AstraZeneca) cannot be found.] Masood.Kamali@igp.uu.se. 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-brain-hippocampus Homo sapiens hippocampus brain_hippocampus brain hippocampus pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Gu G.J., Wu D., Lund H., Sunnemark D., Kvist A.J., Milner R., Eckersley S., Nilsson L.N., Agerman K., Landegren U., Kamali-Moghaddam M. Alzheimers - Interactions investigated in the context of Alzheimers disease rhTau 3T3 cells with stable expression of recombinant human "4R" tau protein. TAU has been entered as isoform "8" -P10636-8 (also known as Tau-4, Tau 441 and Tau-F) as this is the only isoform listed in UniProt with a serine at positions 262 and 356. This is a "4R" isoform, has four (4R) ~32 amino acid imperfect repeats in the microtubule binding domain. [The rabbit polyclonal antibody against phosphorylated Ser262 of tau ( AGG5759), AstraZeneca) cannot be found.] Masood.Kamali@igp.uu.se. Accepted 2013-AUG-07 by ORCHARD 09-Aug-2013: Contacted by IntAct-Help. J. Alzheimers Dis. (1387-2877) 2013 Only protein-protein interactions imex curation p10636-8 Microtubule-associated protein tau Tau-4 MAPT MAPTL MTBT1 TAU Neurofibrillary tangle protein Paired helical filament-tau 2N4R protein protein human Homo sapiens Human MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL DIP protein P10636-8 original sequence version: 206 DIP protein P10636-8 original sequence version: 203 DIP protein P10636-8 original sequence version: 166 DIP protein P10636-8 original sequence version: 218 835A8706D847A8CC mark2_human Serine/threonine-protein kinase MARK2 MAP/microtubule affinity-regulating kinase 2 ELKL motif kinase 1 PAR1 homolog MARK2 EMK1 PAR1 homolog b protein protein human Homo sapiens Human MSSARTPLPTLNERDTEQPTLGHLDSKPSSKSNMIRGRNSATSADEQPHIGNYRLLKTIGKGNFAKVKLARHILTGKEVAVKIIDKTQLNSSSLQKLFREVRIMKVLNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYLVAHGRMKEKEARAKFRQIVSAVQYCHQKFIVHRDLKAENLLLDADMNIKIADFGFSNEFTFGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKYRIPFYMSTDCENLLKKFLILNPSKRGTLEQIMKDRWMNVGHEDDELKPYVEPLPDYKDPRRTELMVSMGYTREEIQDSLVGQRYNEVMATYLLLGYKSSELEGDTITLKPRPSADLTNSSAPSPSHKVQRSVSANPKQRRFSDQAAGPAIPTSNSYSKKTQSNNAENKRPEEDRESGRKASSTAKVPASPLPGLERKKTTPTPSTNSVLSTSTNRSRNSPLLERASLGQASIQNGKDSLTMPGSRASTASASAAVSAARPRQHQKSMSASVHPNKASGLPPTESNCEVPRPSTAPQRVPVASPSAHNISSSGGAPDRTNFPRGVSSRSTFHAGQLRQVRDQQNLPYGVTPASPSGHSQGRRGASGSIFSKFTSKFVRRNLSFRFARRNLNEPESKDRVETLRPHVVGSGGNDKEKEEFREAKPRSLRFTWSMKTTSSMEPNEMMREIRKVLDANSCQSELHEKYMLLCMHGTPGHEDFVQWEMEVCKLPRLSLNGVRFKRISGTSMAFKNIASKIANELKL DIP protein Q7KZI7 original sequence version: 53 DIP protein Q7KZI7 original sequence version: 76 DIP protein Q7KZI7 original sequence version: 70 F307BF4DDB543A70 tau_human Microtubule-associated protein tau MAPT MTBT1 TAU Neurofibrillary tangle protein Paired helical filament-tau MAPTL protein protein human Homo sapiens Human MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQEPESGKVVQEGFLREPGPPGLSHQLMSGMPGAPLLPEGPREATRQPSGTGPEDTEGGRHAPELLKHQLLGDLHQEGPPLKGAGGKERPGSKEEVDEDRDVDESSPQDSPPSKASPAQDGRPPQTAAREATSIPGFPAEGAIPLPVDFLSKVSTEIPASEPDGPSVGRAKGQDAPLEFTFHVEITPNVQKEQAHSEEHLGRAAFPGAPGEGPEARGPSLGEDTKEADLPEPSEKQPAAAPRGKPVSRVPQLKARMVSKSKDGTGSDDKKAKTSTRSSAKTLKNRPCLSPKHPTPGSSDPLIQPSSPAVCPEPPSSPKYVSSVTSRTGSSGAKEMKLKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL DIP protein P10636 original sequence version: 125 DIP protein P10636 original sequence version: 210 D46C66CDBCD196E8 mapt-mark2 1 3 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 3A Staurosporine, a protein kinase inhibitor MARK2 phosphorylates tau on Ser262 mapt-mark2-1 2 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 5 Increased interaction was found in the hippocampal CA1/2-field for AD as compared to non-AD brains.