IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The expression of epidermal lipoxygenases and transglutaminase-1 is perturbed by NIPAL4 mutations: indications of a common metabolic pathway essential for skin barrier homeostasis. The expression of epidermal lipoxygenases and transglutaminase-1 is perturbed by NIPAL4 mutations: indications of a common metabolic pathway essential for skin barrier homeostasis. J. Invest. Dermatol. (0022-202X) 2012 imex curation Li H., Lorie E.P., Fischer J., Vahlquist A., Torma H. Affinomics - Interactions curated for the Affinomics consortium. hans.torma@medsci.uu.se 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-skin Homo sapiens skin skin pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Li H., Loriè E.P., Fischer J., Vahlquist A., Törmä H. Affinomics - Interactions curated for the Affinomics consortium. Patient mutations effects have been introduced as comments rather than features due to the impossibility to assign the effect to a given mutation (mutations were mostly heterozygous). hans.torma@medsci.uu.se Accepted 2013-SEP-10 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Invest. Dermatol. (0022-202X) 2012 Only protein-protein interactions imex curation You'll need the pdfs to review this one, it is not publicly available. I sent them to you by email. The expression of epidermal lipoxygenases and transglutaminase-1 is perturbed by NIPAL4 mutations: indications of a common metabolic pathway essential for skin barrier homeostasis. The expression of epidermal lipoxygenases and transglutaminase-1 is perturbed by NIPAL4 mutations: indications of a common metabolic pathway essential for skin barrier homeostasis. J. Invest. Dermatol. (0022-202X) 2012 imex curation Li H., Lorie E.P., Fischer J., Vahlquist A., Torma H. Affinomics - Interactions curated for the Affinomics consortium. hans.torma@medsci.uu.se 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions human-skin Homo sapiens skin skin anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li H., Loriè E.P., Fischer J., Vahlquist A., Törmä H. Affinomics - Interactions curated for the Affinomics consortium. hans.torma@medsci.uu.se Accepted 2013-SEP-10 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. J. Invest. Dermatol. (0022-202X) 2012 Only protein-protein interactions imex curation You'll need the pdfs to review this one, it is not publicly available. I sent them to you by email. lx12b_human Arachidonate 12-lipoxygenase, 12R-type ALOX12B Epidermis-type lipoxygenase 12 protein protein human Homo sapiens Human MATYKVRVATGTDLLSGTRDSISLTIVGTQGESHKQLLNHFGRDFATGAVGQYTVQCPQDLGELIIIRLHKERYAFFPKDPWYCNYVQICAPNGRIYHFPAYQWMDGYETLALREATGKTTADDSLPVLLEHRKEEIRAKQDFYHWRVFLPGLPSYVHIPSYRPPVRRHRNPNRPEWNGYIPGFPILINFKATKFLNLNLRYSFLKTASFFVRLGPMALAFKVRGLLDCKHSWKRLKDIRKIFPGKKSVVSEYVAEHWAEDTFFGYQYLNGVNPGLIRRCTRIPDKFPVTDDMVAPFLGEGTCLQAELEKGNIYLADYRIMEGIPTVELSGRKQHHCAPLCLLHFGPEGKMMPIAIQLSQTPGPDCPIFLPSDSEWDWLLAKTWVRYAEFYSHEAIAHLLETHLIAEAFCLALLRNLPMCHPLYKLLIPHTRYTVQINSIGRAVLLNEGGLSAKGMSLGVEGFAGVMVRALSELTYDSLYLPNDFVERGVQDLPGYYYRDDSLAVWNALEKYVTEIITYYYPSDAAVEGDPELQSWVQEIFKECLLGRESSGFPRCLRTVPELIRYVTIVIYTCSAKHAAVNTGQMEFTAWMPNFPASMRNPPIQTKGLTTLETFMDTLPDVKTTCITLLVLWTLSREPDDRRPLGHFPDIHFVEEAPRRSIEAFRQRLNQISHDIRQRNKCLPIPYYYLDPVLIENSISI C334075759F8B077 loxe3_human Hydroperoxide isomerase ALOXE3 Hydroperoxy icosatetraenoate dehydratase ALOXE3 Epidermis-type lipoxygenase 3 Hydroperoxy dehydratase ALOXE3 Hydroperoxy icosatetraenoate isomerase protein protein human Homo sapiens Human MAVYRLCVTTGPYLRAGTLDNISVTLVGTCGESPKQRLDRMGRDFAPGSVQKYKVRCTAELGELLLLRVHKERYAFFRKDSWYCSRICVTEPDGSVSHFPCYQWIEGYCTVELRPGTARTICQDSLPLLLDHRTRELRARQECYRWKIYAPGFPCMVDVNSFQEMESDKKFALTKTTTCVDQGDSSGNRYLPGFPMKIDIPSLMYMEPNVRYSATKTISLLFNAIPASLGMKLRGLLDRKGSWKKLDDMQNIFWCHKTFTTKYVTEHWCEDHFFGYQYLNGVNPVMLHCISSLPSKLPVTNDMVAPLLGQDTCLQTELERGNIFLADYWILAEAPTHCLNGRQQYVAAPLCLLWLSPQGALVPLAIQLSQTPGPDSPIFLPTDSEWDWLLAKTWVRNSEFLVHENNTHFLCTHLLCEAFAMATLRQLPLCHPIYKLLLPHTRYTLQVNTIARATLLNPEGLVDQVTSIGRQGLIYLMSTGLAHFTYTNFCLPDSLRARGVLAIPNYHYRDDGLKIWAAIESFVSEIVGYYYPSDASVQQDSELQAWTGEIFAQAFLGRESSGFPSRLCTPGEMVKFLTAIIFNCSAQHAAVNSGQHDFGAWMPNAPSSMRQPPPQTKGTTTLKTYLDTLPEVNISCNNLLLFWLVSQEPKDQRPLGTYPDEHFTEEAPRRSIAAFQSRLAQISRDIQERNQGLALPYTYLDPPLIENSVSI BDED1E4ED5CF6783 tgm1_human Protein-glutamine gamma-glutamyltransferase K KTG Transglutaminase K Transglutaminase-1 Epidermal TGase TGM1 protein protein human Homo sapiens Human MMDGPRSDVGRWGGNPLQPPTTPSPEPEPEPDGRSRRGGGRSFWARCCGCCSCRNAADDDWGPEPSDSRGRGSSSGTRRPGSRGSDSRRPVSRGSGVNAAGDGTIREGMLVVNGVDLLSSRSDQNRREHHTDEYEYDELIVRRGQPFHMLLLLSRTYESSDRITLELLIGNNPEVGKGTHVIIPVGKGGSGGWKAQVVKASGQNLNLRVHTSPNAIIGKFQFTVRTQSDAGEFQLPFDPRNEIYILFNPWCPEDIVYVDHEDWRQEYVLNESGRIYYGTEAQIGERTWNYGQFDHGVLDACLYILDRRGMPYGGRGDPVNVSRVISAMVNSLDDNGVLIGNWSGDYSRGTNPSAWVGSVEILLSYLRTGYSVPYGQCWVFAGVTTTVLRCLGLATRTVTNFNSAHDTDTSLTMDIYFDENMKPLEHLNHDSVWNFHVWNDCWMKRPDLPSGFDGWQVVDATPQETSSGIFCCGPCSVESIKNGLVYMKYDTPFIFAEVNSDKVYWQRQDDGSFKIVYVEEKAIGTLIVTKAISSNMREDITYLYKHPEGSDAERKAVETAAAHGSKPNVYANRGSAEDVAMQVEAQDAVMGQDLMVSVMLINHSSSRRTVKLHLYLSVTFYTGVSGTIFKETKKEVELAPGASDRVTMPVAYKEYRPHLVDQGAMLLNVSGHVKESGQVLAKQHTFRLRTPDLSLTLLGAAVVGQECEVQIVFKNPLPVTLTNVVFRLEGSGLQRPKILNVGDIGGNETVTLRQSFVPVRPGPRQLIASLDSPQLSQVHGVIQVDVAPAPGDGGFFSDAGGDSHLGETIPMASRGGA 4732F28234F5D5F1 alox12b-aloxe3 1 3 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 4a-f, S5a-f, 6e, S10e Interaction is increased in patients with NIPAL4 mutations (homozygous for the missense mutation p.Ala176Asp). alox12b-aloxe3-2 2 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association S6a, S6b aloxe3-tgm1 1 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 5a-f, S7a-f, S8 Interaction is increased in patients with ALOX12B (homozygous for the missense mutation(s) p.Gly462Asp or compound heterozygous for p.Pro422Leu and p.Tyr521Cys) and ALOXE3 (compound heterozygous for the missense mutations p.Arg234X and p.Pro630Leu or compound heterozygous for the donor splice site mutations c.1305+1G>T and c.1305+2A>T) mutations. aloxe12b-tgm1 1 5 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction S9a-f Interaction is increased in patients with ALOX12B mutations (homozygous for the missense mutation(s) p.Gly462Asp or compound heterozygous for p.Pro422Leu and p.Tyr521Cys).