IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Protein kinase CK2 triggers cytosolic zinc signaling pathways by phosphorylation of zinc channel ZIP7. Protein kinase CK2 triggers cytosolic zinc signaling pathways by phosphorylation of zinc channel ZIP7. Sci Signal 2012 imex curation Taylor K.M., Hiscox S., Nicholson R.I., Hogstrand C., Kille P. Affinomics - Interactions curated for the Affinomics consortium. Tamoxifen-resistant (TamR) cells - derived from MCF-7 cells. These cells display an aggressive, invasive phenotype, and have increased levels of zinc and the zinc transporter, ZIP7. This results in an enhanced response to exogenous zinc, which is manifested as a greatly increased growth factor receptor activation, leading to increased growth and invasion. The authors state that the Santa Cruz antibody sc12738 (IAD9) was used to detect CSNK2A1 - the "Genetic Locus" is given as CSNK2A1 (human). taylorkm@cardiff.ac.uk 09-Aug-2013: Contacted by IntAct-Help. The Target of the "CK2alpha" antibody is Gene CSNK2A1. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Taylor K.M., Hiscox S., Nicholson R.I., Hogstrand C., Kille P. Affinomics - Interactions curated for the Affinomics consortium. Tamoxifen-resistant (TamR) cells - derived from MCF-7 cells. These cells display an aggressive, invasive phenotype, and have increased levels of zinc and the zinc transporter, ZIP7. This results in an enhanced response to exogenous zinc, which is manifested as a greatly increased growth factor receptor activation, leading to increased growth and invasion. The authors state that the Santa Cruz antibody sc12738 (IAD9) was used to detect CSNK2A1 - the "Genetic Locus" is given as CSNK2A1 (human). taylorkm@cardiff.ac.uk Accepted 2013-AUG-02 by ORCHARD 09-Aug-2013: Contacted by IntAct-Help. The Target of the "CK2alpha" antibody is Gene CSNK2A1. Sci Signal 2012 Only protein-protein interactions imex curation email sent for antibody cat no to determine specificity - (while top of the Santa Cruz list of abs to CK-alpha are to CSNK2A1, others further down the list are to multiple targets...) Reply promptly received (and reply apologetic) giving the exact antibody used: I am confident it is to CSNKA1 - it you are not, I will delete the publication.... Protein kinase CK2 triggers cytosolic zinc signaling pathways by phosphorylation of zinc channel ZIP7. Protein kinase CK2 triggers cytosolic zinc signaling pathways by phosphorylation of zinc channel ZIP7. Sci Signal 2012 imex curation Taylor K.M., Hiscox S., Nicholson R.I., Hogstrand C., Kille P. Affinomics - Interactions curated for the Affinomics consortium. Tamoxifen-resistant (TamR) cells - derived from MCF-7 cells. These cells display an aggressive, invasive phenotype, and have increased levels of zinc and the zinc transporter, ZIP7. This results in an enhanced response to exogenous zinc, which is manifested as a greatly increased growth factor receptor activation, leading to increased growth and invasion. The authors state that the Santa Cruz antibody sc12738 (IAD9) was used to detect CSNK2A1 - the "Genetic Locus" is given as CSNK2A1 (human). taylorkm@cardiff.ac.uk 09-Aug-2013: Contacted by IntAct-Help. The Target of the "CK2alpha" antibody is Gene CSNK2A1. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Taylor K.M., Hiscox S., Nicholson R.I., Hogstrand C., Kille P. Affinomics - Interactions curated for the Affinomics consortium. Tamoxifen-resistant (TamR) cells - derived from MCF-7 cells. These cells display an aggressive, invasive phenotype, and have increased levels of zinc and the zinc transporter, ZIP7. This results in an enhanced response to exogenous zinc, which is manifested as a greatly increased growth factor receptor activation, leading to increased growth and invasion. The authors state that the Santa Cruz antibody sc12738 (IAD9) was used to detect CSNK2A1 - the "Genetic Locus" is given as CSNK2A1 (human). taylorkm@cardiff.ac.uk Accepted 2013-AUG-02 by ORCHARD 09-Aug-2013: Contacted by IntAct-Help. The Target of the "CK2alpha" antibody is Gene CSNK2A1. Sci Signal 2012 Only protein-protein interactions imex curation email sent for antibody cat no to determine specificity - (while top of the Santa Cruz list of abs to CK-alpha are to CSNK2A1, others further down the list are to multiple targets...) Reply promptly received (and reply apologetic) giving the exact antibody used: I am confident it is to CSNKA1 - it you are not, I will delete the publication.... Protein kinase CK2 triggers cytosolic zinc signaling pathways by phosphorylation of zinc channel ZIP7. Protein kinase CK2 triggers cytosolic zinc signaling pathways by phosphorylation of zinc channel ZIP7. Sci Signal 2012 imex curation Taylor K.M., Hiscox S., Nicholson R.I., Hogstrand C., Kille P. Affinomics - Interactions curated for the Affinomics consortium. Tamoxifen-resistant (TamR) cells - derived from MCF-7 cells. These cells display an aggressive, invasive phenotype, and have increased levels of zinc and the zinc transporter, ZIP7. This results in an enhanced response to exogenous zinc, which is manifested as a greatly increased growth factor receptor activation, leading to increased growth and invasion. The authors state that the Santa Cruz antibody sc12738 (IAD9) was used to detect CSNK2A1 - the "Genetic Locus" is given as CSNK2A1 (human). taylorkm@cardiff.ac.uk 09-Aug-2013: Contacted by IntAct-Help. The Target of the "CK2alpha" antibody is Gene CSNK2A1. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Taylor K.M., Hiscox S., Nicholson R.I., Hogstrand C., Kille P. Affinomics - Interactions curated for the Affinomics consortium. Tamoxifen-resistant (TamR) cells - derived from MCF-7 cells. These cells display an aggressive, invasive phenotype, and have increased levels of zinc and the zinc transporter, ZIP7. This results in an enhanced response to exogenous zinc, which is manifested as a greatly increased growth factor receptor activation, leading to increased growth and invasion. The authors state that the Santa Cruz antibody sc12738 (IAD9) was used to detect CSNK2A1 - the "Genetic Locus" is given as CSNK2A1 (human). taylorkm@cardiff.ac.uk Accepted 2013-AUG-02 by ORCHARD 09-Aug-2013: Contacted by IntAct-Help. The Target of the "CK2alpha" antibody is Gene CSNK2A1. Sci Signal 2012 Only protein-protein interactions imex curation email sent for antibody cat no to determine specificity - (while top of the Santa Cruz list of abs to CK-alpha are to CSNK2A1, others further down the list are to multiple targets...) Reply promptly received (and reply apologetic) giving the exact antibody used: I am confident it is to CSNKA1 - it you are not, I will delete the publication.... Protein kinase CK2 triggers cytosolic zinc signaling pathways by phosphorylation of zinc channel ZIP7. Protein kinase CK2 triggers cytosolic zinc signaling pathways by phosphorylation of zinc channel ZIP7. Sci Signal 2012 imex curation Taylor K.M., Hiscox S., Nicholson R.I., Hogstrand C., Kille P. Affinomics - Interactions curated for the Affinomics consortium. Tamoxifen-resistant (TamR) cells - derived from MCF-7 cells. These cells display an aggressive, invasive phenotype, and have increased levels of zinc and the zinc transporter, ZIP7. This results in an enhanced response to exogenous zinc, which is manifested as a greatly increased growth factor receptor activation, leading to increased growth and invasion. The authors state that the Santa Cruz antibody sc12738 (IAD9) was used to detect CSNK2A1 - the "Genetic Locus" is given as CSNK2A1 (human). taylorkm@cardiff.ac.uk 09-Aug-2013: Contacted by IntAct-Help. The Target of the "CK2alpha" antibody is Gene CSNK2A1. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Taylor K.M., Hiscox S., Nicholson R.I., Hogstrand C., Kille P. Affinomics - Interactions curated for the Affinomics consortium. Tamoxifen-resistant (TamR) cells - derived from MCF-7 cells. These cells display an aggressive, invasive phenotype, and have increased levels of zinc and the zinc transporter, ZIP7. This results in an enhanced response to exogenous zinc, which is manifested as a greatly increased growth factor receptor activation, leading to increased growth and invasion. The authors state that the Santa Cruz antibody sc12738 (IAD9) was used to detect CSNK2A1 - the "Genetic Locus" is given as CSNK2A1 (human). taylorkm@cardiff.ac.uk Accepted 2013-AUG-02 by ORCHARD 09-Aug-2013: Contacted by IntAct-Help. The Target of the "CK2alpha" antibody is Gene CSNK2A1. Sci Signal 2012 Only protein-protein interactions imex curation email sent for antibody cat no to determine specificity - (while top of the Santa Cruz list of abs to CK-alpha are to CSNK2A1, others further down the list are to multiple targets...) Reply promptly received (and reply apologetic) giving the exact antibody used: I am confident it is to CSNKA1 - it you are not, I will delete the publication.... s39a7_human Zinc transporter SLC39A7 Solute carrier family 39 member 7 Histidine-rich membrane protein Ke4 SLC39A7 HKE4 RING5 Zrt-, Irt-like protein 7 Really interesting new gene 5 protein protein protein human Homo sapiens Human MARGLGAPHWVAVGLLTWATLGLLVAGLGGHDDLHDDLQEDFHGHSHRHSHEDFHHGHSHAHGHGHTHESIWHGHTHDHDHGHSHEDLHHGHSHGYSHESLYHRGHGHDHEHSHGGYGESGAPGIKQDLDAVTLWAYALGATVLISAAPFFVLFLIPVESNSPRHRSLLQILLSFASGGLLGDAFLHLIPHALEPHSHHTLEQPGHGHSHSGQGPILSVGLWVLSGIVAFLVVEKFVRHVKGGHGHSHGHGHAHSHTRGSHGHGRQERSTKEKQSSEEEEKETRGVQKRRGGSTVPKDGPVRPQNAEEEKRGLDLRVSGYLNLAADLAHNFTDGLAIGASFRGGRGLGILTTMTVLLHEVPHEVGDFAILVQSGCSKKQAMRLQLLTAVGALAGTACALLTEGGAVGSEIAGGAGPGWVLPFTAGGFIYVATVSVLPELLREASPLQSLLEVLGLLGGVIMMVLIAHLE 6504A1EF5AA6A5B9 csk21_human Casein kinase II subunit alpha CSNK2A1 CK2A1 protein protein human Homo sapiens Human MSGPVPSRARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSANMMSGISSVPTPSPLGPLAGSPVIAAANPLGMPVPAAAGAQQ DIP protein P68400 original sequence version: 88 DIP protein P68400 original sequence version: 78 DIP protein P68400 original sequence version: 100 DIP protein P68400 original sequence version: 110 DIP protein P68400 original sequence version: 125 DIP protein P68400 original sequence version: 136 D3B6F5D13FF7422D slc39a7-csnk2a1 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 1C Zinc csnk2a1-slc39a7 2 5 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 1E, Supp1, 4B, Supp 5A, 5 There was an increase in association of ZIP7 and CK2 at 2 min, with their association returning to prestimulation levels after 10 min and showing a significant (P = 0.05) decrease in their association compared to the starting value by 20 min. Treatment with EGF and ionomycin also produced CK2 association with ZIP7 (Fig. 4B and fig. S5A). The time course of ZIP7-CK2 interactions was identical to that observed with exogenous zinc. Zinc, EGF, ionomycin (Ca ionophore), sodium pyrithione (Zn ionophore) DMAT and TBB - CK2 inhibitors siRNAs against ZIP7 or CK2 slc39a7-csnk2a1-1 3 5 unspecified role unspecified role bait bait Q92504:p.Ser275_Ser276delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain SS AA Thu Jul 07 12:07:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was ser275ala,ser276ala Fri Jul 22 11:12:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'ser275ala,ser276ala' Wed May 02 18:55:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser_ser275-276ala_ala' Tue Jun 30 18:21:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser275_Ser276delinsAlaAla' c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role prey prey physical association physical association 3C Zinc csnk2a1-slc39a7-1 4 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component c_terminus v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Supp 3C Zinc