IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SAF-A forms a complex with BRG1 and both components are required for RNA polymerase II mediated transcription. SAF-A forms a complex with BRG1 and both components are required for RNA polymerase II mediated transcription. PLoS ONE 2011 imex curation Vizlin-Hodzic D., Runnberg R., Ryme J., Simonsson S., Simonsson T. Affinomics - Interactions curated for the Affinomics consortium. SAF-a-pol II interaction depicted in figure S1 could not be curated because the specific subunit of RNA polymerase II involved could not be identified. Stina.simonsson@medkem.gu.se, tomas.simonsson@gu.se 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells confocal microscopy confocal microscopy immunostaining immunostaining Vizlin-Hodzic D., Runnberg R., Ryme J., Simonsson S., Simonsson T. Affinomics - Interactions curated for the Affinomics consortium. SAF-a-pol II interaction depicted in figure S1 could not be curated because the specific subunit of RNA polymerase II involved could not be identified. Stina.simonsson@medkem.gu.se, tomas.simonsson@gu.se Accepted 2013-DEC-19 by ORCHARD PLoS ONE 2011 Only protein-protein interactions imex curation SAF-A forms a complex with BRG1 and both components are required for RNA polymerase II mediated transcription. SAF-A forms a complex with BRG1 and both components are required for RNA polymerase II mediated transcription. PLoS ONE 2011 imex curation Vizlin-Hodzic D., Runnberg R., Ryme J., Simonsson S., Simonsson T. Affinomics - Interactions curated for the Affinomics consortium. SAF-a-pol II interaction depicted in figure S1 could not be curated because the specific subunit of RNA polymerase II involved could not be identified. Stina.simonsson@medkem.gu.se, tomas.simonsson@gu.se 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Vizlin-Hodzic D., Runnberg R., Ryme J., Simonsson S., Simonsson T. Affinomics - Interactions curated for the Affinomics consortium. SAF-a-pol II interaction depicted in figure S1 could not be curated because the specific subunit of RNA polymerase II involved could not be identified. Stina.simonsson@medkem.gu.se, tomas.simonsson@gu.se Accepted 2013-DEC-19 by ORCHARD PLoS ONE 2011 Only protein-protein interactions imex curation SAF-A forms a complex with BRG1 and both components are required for RNA polymerase II mediated transcription. SAF-A forms a complex with BRG1 and both components are required for RNA polymerase II mediated transcription. PLoS ONE 2011 imex curation Vizlin-Hodzic D., Runnberg R., Ryme J., Simonsson S., Simonsson T. Affinomics - Interactions curated for the Affinomics consortium. SAF-a-pol II interaction depicted in figure S1 could not be curated because the specific subunit of RNA polymerase II involved could not be identified. Stina.simonsson@medkem.gu.se, tomas.simonsson@gu.se 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Vizlin-Hodzic D., Runnberg R., Ryme J., Simonsson S., Simonsson T. Affinomics - Interactions curated for the Affinomics consortium. SAF-a-pol II interaction depicted in figure S1 could not be curated because the specific subunit of RNA polymerase II involved could not be identified. Stina.simonsson@medkem.gu.se, tomas.simonsson@gu.se Accepted 2013-DEC-19 by ORCHARD PLoS ONE 2011 Only protein-protein interactions imex curation smca4_mouse Transcription activator BRG1 Smarca4 Baf190a Brg1 Snf2b Snf2l4 ATP-dependent helicase SMARCA4 BRG1-associated factor 190A Protein brahma homolog 1 SNF2-beta SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 protein protein mouse Mus musculus Mouse MSTPDPPLGGTPRPGPSPGPGPSPGAMLGPSPGPSPGSAHSMMGPSPGPPSAGHPMPTQGPGGYPQDNMHQMHKPMESMHEKGMPDDPRYNQMKGMGMRSGAHTGMAPPPSPMDQHSQGYPSPLGGSEHASSPVPASGPSSGPQMSSGPGGAPLDGSDPQALGQQNRGPTPFNQNQLHQLRAQIMAYKMLARGQPLPDHLQMAVQGKRPMPGMQQQMPTLPPPSVSATGPGPGPGPGPGPGPGPAPPNYSRPHGMGGPNMPPPGPSGVPPGMPGQPPGGPPKPWPEGPMANAAAPTSTPQKLIPPQPTGRPSPAPPAVPPAASPVMPPQTQSPGQPAQPAPLVPLHQKQSRITPIQKPRGLDPVEILQEREYRLQARIAHRIQELENLPGSLAGDLRTKATIELKALRLLNFQRQLRQEVVVCMRRDTALETALNAKAYKRSKRQSLREARITEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFREYHRSVTGKLQKLTKAVATYHANTEREQKKENERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAYLLQQTDEYVANLTELVRQHKAAQVAKEKKKKKKKKKAENAEGQTPAIGPDGEPLDETSQMSDLPVKVIHVESGKILTGTDAPKAGQLEAWLEMNPGYEVAPRSDSEESGSEEEEEEEEEEQPQPAQPPTLPVEEKKKIPDPDSDDVSEVDARHIIENAKQDVDDEYGVSQALARGLQSYYAVAHAVTERVDKQSALMVNGVLKQYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTIALITYLMEHKRINGPFLIIVPLSTLSNWAYEFDKWAPSVVKVSYKGSPAARRAFVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRLLLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRLKKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESFSEHLGFTGGIVQGLDLYRASGKFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAYRGFKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEQDEEEDEVPDDETVNQMIARHEEEFDLFMRMDLDRRREEARNPKRKPRLMEEDELPSWIIKDDAEVERLTCEEEEEKMFGRGSRHRKEVDYSDSLTEKQWLKAIEEGTLEEIEEEVRQKKSSRKRKRDSEAGSSTPTTSTRSRDKDEESKKQKKRGRPPAEKLSPNPPNLTKKMKKIVDAVIKYKDSSGRQLSEVFIQLPSRKELPEYYELIRKPVDFKKIKERIRNHKYRSLNDLEKDVMLLCQNAQTFNLEGSLIYEDSIVLQSVFTSVRQKIEKEDDSEGEESEEEEEGEEEGSESESRSVKVKIKLGRKEKAQDRLKGGRRRPSRGSRAKPVVSDDDSEEEQEEDRSGSGSEED DIP protein Q3TKT4 original sequence version: 82 DIP protein Q3TKT4 original sequence version: 56 DIP protein Q3TKT4 original sequence version: 43 DIP protein Q3TKT4 original sequence version: 75 C23804ED858F98FE hnrpu_mouse Heterogeneous nuclear ribonucleoprotein U Hnrpu Hnrnpu Scaffold-attachment factor A protein protein mouse Mus musculus Mouse MSSSPVNVKKLKVSELKEELKKRRLSDKGLKADLMDRLQAALDNEAGGRPAMEPGNGSLDLGGDAAGRSGAGLEQEAAAGAEDDEEEEGIAALDGDQMELGEENGAAGAADAGAMEEEEAASEDENGDDQGFQEGEDELGDEEEGAGDENGHGEQQSQPPAAAAQQQPSQQRGAGKEAAGKSSGPTSLFAVTVAPPGARQGQQQAGGDGKTEQKGGDKKRGVKRPREDHGRGYFEYIEENKYSRAKSPQPPVEEEDEHFDDTVVCLDTYNCDLHFKISRDRLSASSLTMESFAFLWAGGRASYGVSKGKVCFEMKVTEKIPVRHLYTKDIDIHEVRIGWSLTTSGMLLGEEEFSYGYSLKGIKTCNCETEDYGEKFDENDVITCFANFETDEVELSYAKNGQDLGVAFKISKEVLADRPLFPHVLCHNCAVEFNFGQKEKPYFPIPEDCTFIQNVPLEDRVRGPKGPEEKKDCEVVMMIGLPGAGKTTWVTKHAAENPGKYNILGTNTIMDKMMVAGFKKQMADTGKLNTLLQRAPQCLGKFIEIAARKKRNFILDQTNVSAAAQRRKMCLFAGFQRKAVVVCPKDEDYKQRTQKKAEVEGKDLPEHAVLKMKGNFTLPEVAECFDEITYVELQKEEAQKLLEQYKEESKKALPPEKKQNTGSKKSNKNKSGKNQFNRGGGHRGRGGFNMRGGNFRGGAPGNRGGYNRRGNMPQRGGGGGSGGIGYPYPRGPVFPGRGGYSNRGNYNRGGMPNRGNYNQNFRGRGNNRGYKNQSQGYNQWQQGQFWGQKPWSQHYHQGYY 2BA7C73043F847B2 hnrnpu-smarca4 1 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component colocalization colocalization 1a hnrnpu-smarca4-1 2 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 1b, 2a Treatment of cell lysate with RNase A decreased the interaction. hnrnpu-smarca4-3 3 4 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 1c, 2b, 3 Treatment of cell lysate with RNase A decreased the interaction.