IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. J. Biol. Chem. (0021-9258) 2011 imex curation Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it Accepted 2013-OCT-14 by MGT 28-Oct_2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. J. Biol. Chem. (0021-9258) 2011 imex curation Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. no anna.moroni@unimi.it Accepted 2013-OCT-14 by MGT 28-Oct_2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. J. Biol. Chem. (0021-9258) 2011 imex curation Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it Accepted 2013-OCT-14 by MGT 28-Oct_2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. J. Biol. Chem. (0021-9258) 2011 imex curation Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it Accepted 2013-OCT-14 by MGT 28-Oct_2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. J. Biol. Chem. (0021-9258) 2011 imex curation Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it Accepted 2013-OCT-14 by MGT 28-Oct_2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. J. Biol. Chem. (0021-9258) 2011 imex curation Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it Accepted 2013-OCT-14 by MGT 28-Oct_2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. Tetramerization dynamics of C-terminal domain underlies isoform-specific cAMP gating in hyperpolarization-activated cyclic nucleotide-gated channels. J. Biol. Chem. (0021-9258) 2011 imex curation Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lolicato M., Nardini M., Gazzarrini S., Möller S., Bertinetti D., Herberg F.W., Bolognesi M., Martin H., Fasolini M., Bertrand J.A., Arrigoni C., Thiel G., Moroni A. Affinomics - Interactions curated for the Affinomics consortium. anna.moroni@unimi.it Accepted 2013-OCT-14 by MGT 28-Oct_2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation hcn1_mouse Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 Hcn1 Bcng1 Hac2 Brain cyclic nucleotide-gated channel 1 Hyperpolarization-activated cation channel 2 protein protein mouse Mus musculus Mouse MEGGGKPNSASNSRDDGNSVFPSKAPATGPVAADKRLGTPPGGGAAGKEHGNSVCFKVDGGGGEEPAGSFEDAEGPRRQYGFMQRQFTSMLQPGVNKFSLRMFGSQKAVEKEQERVKTAGFWIIHPYSDFRFYWDLIMLIMMVGNLVIIPVGITFFTEQTTTPWIIFNVASDTVFLLDLIMNFRTGTVNEDSSEIILDPKVIKMNYLKSWFVVDFISSIPVDYIFLIVEKGMDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIFNLIGMMLLLCHWDGCLQFLVPLLQDFPPDCWVSLNEMVNDSWGKQYSYALFKAMSHMLCIGYGAQAPVSMSDLWITMLSMIVGATCYAMFVGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADMRQKIHDYYEHRYQGKIFDEENILSELNDPLREEIVNFNCRKLVATMPLFANADPNFVTAMLSKLRFEVFQPGDYIIREGAVGKKMYFIQHGVAGVITKSSKEMKLTDGSYFGEICLLTKGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLQKFQKDLNTGVFNNQENEILKQIVKHDREMVQAIPPINYPQMTALNCTSSTTTPTSRMRTQSPPVYTATSLSHSNLHSPSPSTQTPQPSAILSPCSYTTAVCSPPIQSPLATRTFHYASPTASQLSLMQQPQQQLPQSQVQQTQTQTQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQPQTPGSSTPKNEVHKSTQALHNTNLTKEVRPLSASQPSLPHEVSTLISRPHPTVGESLASIPQPVAAVHSTGLQAGSRSTVPQRVTLFRQMSSGAIPPNRGVPPAPPPPAAVQRESPSVLNTDPDAEKPRFASNL 56FD5F328DD972E9 hcn4_human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4 HCN4 protein protein human Homo sapiens Human MDKLPPSMRKRLYSLPQQVGAKAWIMDEEEDAEEEGAGGRQDPSRRSIRLRPLPSPSPSAAAGGTESRSSALGAADSEGPARGAGKSSTNGDCRRFRGSLASLGSRGGGSGGTGSGSSHGHLHDSAEERRLIAEGDASPGEDRTPPGLAAEPERPGASAQPAASPPPPQQPPQPASASCEQPSVDTAIKVEGGAAAGDQILPEAEVRLGQAGFMQRQFGAMLQPGVNKFSLRMFGSQKAVEREQERVKSAGFWIIHPYSDFRFYWDLTMLLLMVGNLIIIPVGITFFKDENTTPWIVFNVVSDTFFLIDLVLNFRTGIVVEDNTEIILDPQRIKMKYLKSWFMVDFISSIPVDYIFLIVETRIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVVRIVNLIGMMLLLCHWDGCLQFLVPMLQDFPDDCWVSINNMVNNSWGKQYSYALFKAMSHMLCIGYGRQAPVGMSDVWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPPDTRQRIHDYYEHRYQGKMFDEESILGELSEPLREEIINFNCRKLVASMPLFANADPNFVTSMLTKLRFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKETKLADGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVALDRLDRIGKKNSILLHKVQHDLNSGVFNYQENEIIQQIVQHDREMAHCAHRVQAAASATPTPTPVIWTPLIQAPLQAAAATTSVAIALTHHPRLPAAIFRPPPGSGLGNLGAGQTPRHLKRLQSLIPSALGSASPASSPSQVDTPSSSSFHIQQLAGFSAPAGLSPLLPSSSSSPPPGACGSPSAPTPSAGVAATTIAGFGHFHKALGGSLSSSDSPLLTPLQPGARSPQAAQPSPAPPGARGGLGLPEHFLPPPPSSRSPSSSPGQLGQPPGELSLGLATGPLSTPETPPRQPEPPSLVAGASGGASPVGFTPRGGLSPPGHSPGPPRTFPSAPPRASGSHGSLLLPPASSPPPPQVPQRRGTPPLTPGRLTQDLKLISASQPALPQDGAQTLRRASPHSSGESMAAFPLFPRAGGGSGGSGSSGGLGPPGRPYGAIPGQHVTLPRKTSSGSLPPPLSLFGARATSSGGPPLTAGPQREPGARPEPVRSKLPSNL DIP protein Q9Y3Q4 original sequence version: 122 DIP protein Q9Y3Q4 original sequence version: 131 7EFDD2D69CF1F9D9 3',5'-cyclic gmp 3',5'-cyclic GMP 3',5'-Cyclic GMP cGMP Cyclic GMP Guanosine 3',5'-cyclic monophosphate Guanosine 3',5'-cyclic phosphate Guanosine cyclic monophosphate guanosine 3',5'-(hydrogen phosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ZOOGRGPOEVQQDX-UUOKFMHZSA-N ZOOGRGPOEVQQDX-UUOKFMHZSA-N hcn2_human Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 HCN2 BCNG2 Brain cyclic nucleotide-gated channel 2 protein protein human Homo sapiens Human MDARGGGGRPGESPGATPAPGPPPPPPPAPPQQQPPPPPPPAPPPGPGPAPPQHPPRAEALPPEAADEGGPRGRLRSRDSSCGRPGTPGAASTAKGSPNGECGRGEPQCSPAGPEGPARGPKVSFSCRGAASGPAPGPGPAEEAGSEEAGPAGEPRGSQASFMQRQFGALLQPGVNKFSLRMFGSQKAVEREQERVKSAGAWIIHPYSDFRFYWDFTMLLFMVGNLIIIPVGITFFKDETTAPWIVFNVVSDTFFLMDLVLNFRTGIVIEDNTEIILDPEKIKKKYLRTWFVVDFVSSIPVDYIFLIVEKGIDSEVYKTARALRIVRFTKILSLLRLLRLSRLIRYIHQWEEIFHMTYDLASAVMRICNLISMMLLLCHWDGCLQFLVPMLQDFPRNCWVSINGMVNHSWSELYSFALFKAMSHMLCIGYGRQAPESMTDIWLTMLSMIVGATCYAMFIGHATALIQSLDSSRRQYQEKYKQVEQYMSFHKLPADFRQKIHDYYEHRYQGKMFDEDSILGELNGPLREEIVNFNCRKLVASMPLFANADPNFVTAMLTKLKFEVFQPGDYIIREGTIGKKMYFIQHGVVSVLTKGNKEMKLSDGSYFGEICLLTRGRRTASVRADTYCRLYSLSVDNFNEVLEEYPMMRRAFETVAIDRLDRIGKKNSILLHKVQHDLNSGVFNNQENAIIQEIVKYDREMVQQAELGQRVGLFPPPPPPPQVTSAIATLQQAAAMSFCPQVARPLVGPLALGSPRLVRRPPPGPAPAAASPGPPPPASPPGAPASPRAPRTSPYGGLPAAPLAGPALPARRLSRASRPLSASQPSLPHGAPGPAASTRPASSSTPRLGPTPAARAAAPSPDRRDSASPGAAGGLDPQDSARSRLSSNL DIP protein Q9UL51 original sequence version: 137 4B263E0C06C2A47D 3',5'-cyclic amp 3',5'-cyclic AMP 3',5'-Cyclic AMP adenosine 3',5'-cyclic monophosphate Adenosine 3',5'-cyclic phosphate Adenosine 3',5'-phosphate ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE cAMP Cyclic adenylic acid Cyclic AMP adenosine 3',5'-(hydrogen phosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) hcn1 5 8 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O88704:p.Arg538Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Wed May 02 17:22:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg538glu' Tue Jun 30 17:28:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg538Glu' c-linker domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain range range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component purified purified c-linker domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain range range c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O88704:p.Arg538Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Wed May 02 18:13:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg538glu' Tue Jun 30 16:44:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg538Glu' ecoli Escherichia coli (strain K12) direct interaction direct interaction 3a, 3g, 5a, 5b, 5c cAMP Tetramerization is enhanced by treatment with cAMP, but a small amount of HCN1 tetramerizes even without cAMP. Increasing the salt concentration results in the disappearance of the tetrameric form of the protein. hcn2-5 5 11 unspecified role unspecified role neutral component neutral component purified purified c-linker domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain range range c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component purified purified c-linker domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain range range c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3b, 5b, 5c cAMP hcn4 5 9 unspecified role unspecified role neutral component neutral component purified purified c-linker domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain range range c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-linker domain required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain range range ecoli Escherichia coli (strain K12) direct interaction direct interaction 3c, 5b, 5c cAMP hcn1-1 4 8 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3d, suppl. table 3b cAMP hcn2-5 4 11 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3e, suppl. table 3b cAMP hcn4-1 4 9 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3f, suppl. table 3b cAMP 3__5__cyclic_-hcn4-1 1 12 unspecified role unspecified role bait bait purified purified (6-aminohexyl)amino linker at c8 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role prey prey purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 competitor competitor competitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 3__5__cyclic_-hcn1-1 1 8 unspecified role unspecified role prey prey purified purified cnbd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 competitor competitor competitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role bait bait purified purified (6-aminohexyl)amino linker at c8 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 3__5__cyclic_-hcn2-1 1 11 unspecified role unspecified role prey prey purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait purified purified (6-aminohexyl)amino linker at c8 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 12 competitor competitor competitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 3__5__cyclic_-hcn1-1 6 8 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1, 1a 3__5__cyclic_-hcn2-3 6 11 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1, 1b 3__5__cyclic_-hcn4-1 6 9 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 3__5__cyclic_-hcn1-3 6 8 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 3__5__cyclic_-hcn2-1 6 11 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1, 1b 3__5__cyclic_-hcn2-1 6 10 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 3__5__cyclic_-hcn4-3 6 10 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 3__5__cyclic_-hcn4-1 6 9 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1, 1c 3__5__cyclic_-hcn4-3 6 12 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role neutral component neutral component purified purified mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1, 1c 3__5__cyclic_-hcn1-1 6 12 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1, 1a 3__5__cyclic_-hcn1-3 6 8 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1, 1a 3__5__cyclic_-hcn2-3 6 11 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component purified purified 8-fluoresceinyl group fluorescein fluorescein label fluorescein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 3__5__cyclic_-hcn2-4 3 12 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 3__5__cyclic_-hcn2-4 3 12 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1, S2a, S2b Kd value in the legend of figure S2 is 3.3 uM. 3__5__cyclic_-hcn4-4 3 12 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mbp-his tag tandem tag tandem tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1, S2c, S2d Kd value given in figure S2 is 0.6 uM. 3__5__cyclic_-hcn4-5 7 12 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Table 1 Rwork = 0.19 Rfree = 0.28 2.5A Entry in PDBe has different quaternary assemblies, but the authors mention 4:4 stochiometry in the text, so I have taken that value for the entry. 3__5__cyclic_am-hcn1 7 8 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 2, suppl. table 1 Rwork = 0.2 Rfree = 0.27 2.9A Entry in PDBe has different quaternary assemblies, but the authors mention 4:4 stochiometry in the text, so I have taken that value for the entry. 3__5__cyclic_-hcn2-5 7 11 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 2d, suppl. table 1 Rwork = 0.2 Rfree = 0.27 2.9A 3__5__cyclic_-hcn1-1 2 8 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3h, suppl. table 4 3__5__cyclic_-hcn2-6 2 11 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction 3h, suppl. table 4 3__5__cyclic_-hcn4-6 2 12 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3h, suppl. table 4 hcn1-4 2 8 unspecified role unspecified role neutral component neutral component purified purified cnbd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3h, suppl. table 4 hcn1-1 2 8 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3h, suppl. table 4 hcn2 2 11 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3h, suppl. table 4 hcn4 2 9 unspecified role unspecified role neutral component neutral component purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3h, suppl. table 4 3__5__cyclic_am-hcn4 1 9 unspecified role unspecified role prey prey purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait purified purified (6-aminohexyl)amino linker at c8 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 3__5__cyclic_-hcn1-4 1 8 unspecified role unspecified role prey prey purified purified cnbd domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait purified purified (6-aminohexyl)amino linker at c8 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 3__5__cyclic_am-hcn2 1 11 unspecified role unspecified role prey prey purified purified c-linker and cnbd domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait purified purified (6-aminohexyl)amino linker at c8 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1