IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ EGFR nuclear translocation modulates DNA repair following cisplatin and ionizing radiation treatment. EGFR nuclear translocation modulates DNA repair following cisplatin and ionizing radiation treatment. Cancer Res. (0008-5472) 2011 imex curation Liccardi G., Hartley J.A., Hochhauser D. Affinomics - Interactions curated for the Affinomics consortium. email sent requesting origin of WT EGFR - sorry I marked this for checking before I sent the email!!! - date 5/6/14- (I doubt there will be a reply....) Mutants of the NLS (nuclear localization signal) have been entered as Human in origin, as the sequence numbering -amino acids 645 to 657 (RRRHIVRKLLRR) can only refer to the numbering of the mature human protein, and Reference 22 also only refers to the Human NLS. The "wt" form of EGFR has also been entered as Human. Amino acid numbering for the NLS has been altered to aas 669-681 for the full length protein. The numbering for the L858R mutant appears to already correspond to that of the full length protein. d.hochhauser@ucl.ac.uk 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Liccardi G., Hartley J.A., Hochhauser D. Affinomics - Interactions curated for the Affinomics consortium. email sent requesting origin of WT EGFR - sorry I marked this for checking before I sent the email!!! - date 5/6/14- (I doubt there will be a reply....) Mutants of the NLS (nuclear localization signal) have been entered as Human in origin, as the sequence numbering -amino acids 645 to 657 (RRRHIVRKLLRR) can only refer to the numbering of the mature human protein, and Reference 22 also only refers to the Human NLS. The "wt" form of EGFR has also been entered as Human. Amino acid numbering for the NLS has been altered to aas 669-681 for the full length protein. The numbering for the L858R mutant appears to already correspond to that of the full length protein. d.hochhauser@ucl.ac.uk Accepted 2014-JUN-18 by ORCHARD Cancer Res. (0008-5472) 2011 Only protein-protein interactions imex curation EGFR nuclear translocation modulates DNA repair following cisplatin and ionizing radiation treatment. EGFR nuclear translocation modulates DNA repair following cisplatin and ionizing radiation treatment. Cancer Res. (0008-5472) 2011 imex curation Liccardi G., Hartley J.A., Hochhauser D. Affinomics - Interactions curated for the Affinomics consortium. email sent requesting origin of WT EGFR - sorry I marked this for checking before I sent the email!!! - date 5/6/14- (I doubt there will be a reply....) Mutants of the NLS (nuclear localization signal) have been entered as Human in origin, as the sequence numbering -amino acids 645 to 657 (RRRHIVRKLLRR) can only refer to the numbering of the mature human protein, and Reference 22 also only refers to the Human NLS. The "wt" form of EGFR has also been entered as Human. Amino acid numbering for the NLS has been altered to aas 669-681 for the full length protein. The numbering for the L858R mutant appears to already correspond to that of the full length protein. d.hochhauser@ucl.ac.uk 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Liccardi G., Hartley J.A., Hochhauser D. Affinomics - Interactions curated for the Affinomics consortium. email sent requesting origin of WT EGFR - sorry I marked this for checking before I sent the email!!! - date 5/6/14- (I doubt there will be a reply....) Mutants of the NLS (nuclear localization signal) have been entered as Human in origin, as the sequence numbering -amino acids 645 to 657 (RRRHIVRKLLRR) can only refer to the numbering of the mature human protein, and Reference 22 also only refers to the Human NLS. The "wt" form of EGFR has also been entered as Human. Amino acid numbering for the NLS has been altered to aas 669-681 for the full length protein. The numbering for the L858R mutant appears to already correspond to that of the full length protein. d.hochhauser@ucl.ac.uk Accepted 2014-JUN-18 by ORCHARD Cancer Res. (0008-5472) 2011 Only protein-protein interactions imex curation EGFR nuclear translocation modulates DNA repair following cisplatin and ionizing radiation treatment. EGFR nuclear translocation modulates DNA repair following cisplatin and ionizing radiation treatment. Cancer Res. (0008-5472) 2011 imex curation Liccardi G., Hartley J.A., Hochhauser D. Affinomics - Interactions curated for the Affinomics consortium. email sent requesting origin of WT EGFR - sorry I marked this for checking before I sent the email!!! - date 5/6/14- (I doubt there will be a reply....) Mutants of the NLS (nuclear localization signal) have been entered as Human in origin, as the sequence numbering -amino acids 645 to 657 (RRRHIVRKLLRR) can only refer to the numbering of the mature human protein, and Reference 22 also only refers to the Human NLS. The "wt" form of EGFR has also been entered as Human. Amino acid numbering for the NLS has been altered to aas 669-681 for the full length protein. The numbering for the L858R mutant appears to already correspond to that of the full length protein. d.hochhauser@ucl.ac.uk 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Liccardi G., Hartley J.A., Hochhauser D. Affinomics - Interactions curated for the Affinomics consortium. email sent requesting origin of WT EGFR - sorry I marked this for checking before I sent the email!!! - date 5/6/14- (I doubt there will be a reply....) Mutants of the NLS (nuclear localization signal) have been entered as Human in origin, as the sequence numbering -amino acids 645 to 657 (RRRHIVRKLLRR) can only refer to the numbering of the mature human protein, and Reference 22 also only refers to the Human NLS. The "wt" form of EGFR has also been entered as Human. Amino acid numbering for the NLS has been altered to aas 669-681 for the full length protein. The numbering for the L858R mutant appears to already correspond to that of the full length protein. d.hochhauser@ucl.ac.uk Accepted 2014-JUN-18 by ORCHARD Cancer Res. (0008-5472) 2011 Only protein-protein interactions imex curation prkdc_mouse DNA-dependent protein kinase catalytic subunit Prkdc Xrcc7 p460 protein protein mouse Mus musculus Mouse MAEEGTGVRCWLLQLQEFLSAADRCSAAGASYQLIRSLGQECVLSTSSAVQALQISLVFSRDFGLLVFIRKSLSIEDFRDCREEALKFLCVFLEKIDQKVMHYSLDIKNTCTSVYTKDRTAKCKIPALDLLIKLLQILRSTRLMDEFKIGELFNKFYGELASKSKLPDTVLEKVYELLGVLGEVHPSEMINHSENLFRAFLGELKTQMTSTVREPKFPVLAGCLKGLSSLLCNFTKSMEEDPQTSKEIFGFTFKAIRPQIEMKRYAVPLAGLRLLTLHASQFTACLLDNYITLFEVLSKWCSHTNVELKKAAHSALESFLRQISFTVAEDAELHKSRLKYFMEQFYGIIRNTDSNNKELAIAIRGYGLFAGPCKVINAKDVDFMYVELIQRCKQMFLTHADASEDHVYQMPSFLQSIASVLLYLDTVPEVYTPVLEHLMVVQIDSFPQYSPKMQLVCCKAIIKLFLALSEKGPVHWNCISAVVHQGLIRICSKPVVLQKDVESRSDNRSASEEVRTGRWKVPTYKDYVDLFQHLLGCDQMEDFILGDETFLFVNSSLKSLNHLLYDEFIRSVLKIVEKLDLTLEKQTVGEQEDGSTADVWVIPTSDPAANLHPAKPSDFSALINLVEFCREILPRKHVGFFEPWVYSFAYELILQSTRLPLISGFYKLLSIAVKNARKIKYFEGISPKSLKHSPEDTEKYSCFALFAKFGKEVSVKMKQYKDELLASCLTFVLSLPHDIIELDVRAYVPALQMAFKLGLSHMPLAEIGLHALKEWSVHIDKSILQPYYKDILPCLDGYLNTSTLSDETKSHWGLSALSRAAQKGFNRHVVKHLKRTRNSSPDEALSLEEIRIKVVQILGSLGGQINKSLVTATSGERMKKYVAWDAERRLSFAVPFREMKPVIYLDVFLPRVTELALSASDRQTKVAACELLHSMVMFMLGRATQMPEGQGLPPMYQLYKHTFPVLLQLACDVDQVTRQLYEPLVMQLIHWLTNNKKFESQDTVALLEAILDGIVDPVDSTLRDFCGRCVQEFLKWSIKQTTPQQQEKSPVNSKSLFKRLYSLALHPNAFKRLGAALAFNHIYKEFREEGSLVEQFVFEALVTYMESLALAHEDEKSLGTVQQCCDAIDHLRRIIEKKHVSLNKAKKRRLPQGFPPLTSLCLLDLVEWLLAHCGRPQTECRHKSMELFYKFVPLLPGNKSPSLWLKDLIKKKGISFLINTFEGGASSSDQPAGILAQPTLVYLQGPISLRGVLQWLDLLLAALECYNTFIEKETVQGQEVLGAEVQSSLLKSVAFFLESIATHSARAVEQRFGSGAPGPPSLHEEEKYNYSKCTVLVRIMEFTTTLLIASPEDCKLLEKDLCNTNLMQVLVKMICEPMSLGFNIGDVQVMNHLPSICVNLLKALRKSPYRDMLETHLKEKVTVQSVEELCSINLCSSGARQERSKLLSILSACKQLHKAGFSHVISPSQSTALNHSVGMRLLSLVYKGIVPAEERQCLQSLDPSCKSLANGLLELAFGFGGLCDHLVSLLLNSAMLSTQYLGSSQRNISFSHGEYFYSLFSEVINSELLKNLDIAVSRLMESSSDNPKMVSTVLNGMLDTSFRDRAVQKHQGLKLATAILQNWRKCDSWWAPDSAPESKTTVLSLLAKMLQIDSALSFDTNHSSFSEIFTTYASLLADTKLGLHLKGQAIILLPFFTSLREGSLENLKHILEKLIVCNFPMKSDEFPPDSLKYNNYVDCMKKFLDALELSQSPMLFQLMTDILCREQRHIMEELFQTTFKRIARQSPCVTQLNLLESVYTMFRKADLPSNVTRQAFVDRSLLTLLWHCDLDTLKEFFSRIVVDAIDVLKSRFTKLNEFTFDTQITKKMCYYKMLAVMYSRLLKDDVHSKEAKINQAFHGSRVAEGNELTKTLLKLCHDAFTENMVGESQLLEKRRLYHCAAYNCAISLISCVFNELKFYQGFLFNEKPEKNLFIFENLIDLKRCYTFPIEVEVPMERKKKYIEIRKEARDAANGASGSPHYMSSLSYLTDSSLSEEMSQFDFSTGVQSYSYSSQDRKPTTGHFQRREHQDSMTQDDIMELEMDELNQHECMAPMIALIKHMQRNVIAPKGEEGSIPKDLPPWMKFLHDKLGNASVSLNIRLFLAKLVINTEEVFRPYAKHWLSPLLQLAVCENNREGIHYMMVEIVATILSWTGLATPTGVPKDEVLANRLLRFLMKHVFHPKRAVFRHNLEIIKTLVECWKECLSIPYRLIFEKFSHKDPNSKDNSVGIQLLGIVIANNLPPYDPNCDITSAMYFEALVNNMSFVKYKEVYAAAAEVLGLILQYITERKHVIAELVCELVIKQLKQHQNTMEDKFIVCLNKIAKGFPPLADRFLNALFFLLPKFHGVMKTLCLEVVLCRAEEITGLYLQLKSKDFLQVMRHRDDERQKVCLDIVYKMVAKLKPIELRELLNPVVEFVSHPSPTCREQMYNILMWIHDNYRDQESQNDEDSQEIFKLAKDVLIQGLIDENVGLQLIIRNFWSHETRLPSNTLDRLLALNSLYSPKIEVHFLSLATNFLLEMTRMSPDYLNPIFEHPLSECEFQEYTIDPDWRFRSTVLTPMFIETQASPSILHTQTQEGPLSDQRQKPGQVRATQQQYDFTPTQASVERSSFDWLTGSSIDLLADHTVFSSETLSSSLLFSHKRTEKSQRMSCKSVGPDFGTKKLGLPDDEVDNQVKSGTPSQADILRLRRRFLKDREKLSLLYAKRGLMEQKLEKDIKSEFKMKQDAQVVLYRSYRHGDLPDIQIQHSGLITPLQAVAQKDPIIAKQLFSSLFSGILKEMNKFKTTSEKNIITQNLLQDFNRFLNTTFLFFPPFVSCIQEISCQHPDFLTLDPASVRVGCLASLQQPGGIRLLEEALLRLMPKEPPTKRVRGKTCLPPDVLRWMELAKLYRSIGEYDVLRGIFSSELGTTQDTQNALLAEARSDYCQAAKLYDEALNKLEWVDGEPTEAEKEFWELASLDCYNNLSKWKELEYCSTVNIVSENSLDLSKMWSEPFYQETYLPYVIRSKLKLLLQGEGNQSLLTFVDEAMNKELQKTVLELQYSQELSLLYILQDDIDRATYYIKNGIQIFMQNYSSIDVLLYRSRLAKLQSVQTLAEIEEFLSFICKHGDLSSLGPLRRLLKTWTSRYPDVVTDPMHIWDDIITNRCFFLSKIEERLTAPSGDHSMSVDEDEESIDREVYEPKEDVRCMLQSCRFTMKMKMIESAWKQSNFSLSMKLLKEMHKESKTREIWRVQWLHSYSQLNHCRSHTQSPREQVLNMLKTITLLDESDISNYLNKNIQASCDQSILLGTTCRIMADALSREPACLSDLEENKVNSILTLSGSNAENTETVITGLYQRAFHHLSKAVQSAEEETQLSCWGHEAAAERAHAYMTLVGFCDQQLRKVEESASQKTSAEMEAYPALVVEKMLRALKLNSSEARLKFPRLLQIIEQYSEETLNIMTKEISSIPCWQFIGWISHMMALLDKEEAIAVQHTVEEIADNYPQAIIYPFIISSESYSFKNTSSGHNNKAFVERIKSKLDHGEVIHSFINALDQLSNPDLLFKDWVSDTKDELGKNPVNKKNIEKLYERMYAALGDLRAPGLGPFRRRFIQAFGKEFVKSFGNGGSKLLTMKVDDFCKITGSLLVRMKKDSKLPGNLKEYSPWMSEFKAQFLKNELEIPGQYDGKSKPLPEYHVRISGFDERVKVMLSLRKPKRIVIRGHDEKEYPFLVKGGEDLRQDQRIEQIFEVMNAILSQDAACSQRNMQLRTYRVVPMTSRLGLIEWIENTMTLKDLLLSNMSQEEKVANNSDPKAPIRDYKDWLMKVSGKSDAGAYVLMYSRANRTETVVAFRRRESQVPPDLLKRAFVKMSTSPEAFLALRSHFASSHALLCISHWLLGIGDRHLNNFMVAMETGSVIGIDFGHAFGSATQFLPVPELMPFRLTRQFVSLMLPMKETGLMCTVMVHALRAFRSCAGLLTDTMEIFVKEPSFDWKSFEQTMLRKGGSWIQEINVTEKNWYPQHKIRYAKRKLAGANPAVITCDELYLGHEASSAFRSYTAVARGNRDYNIRAQEPESGLSEETQVKCLVDQATDPNILGRTWEGWEPWM B143922D57F4331E egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA DIP protein P00533 original sequence version: 213 DIP protein P00533 original sequence version: 214 DIP protein P00533 original sequence version: 119 DIP protein P00533 original sequence version: 207 DIP protein P00533 original sequence version: 132 DIP protein P00533-1 original sequence version: 197 DIP protein P00533 original sequence version: 163 DIP protein P00533-1 original sequence version: 159 DIP protein P00533 original sequence version: 184 DIP protein P00533-1 original sequence version: 174 DIP protein P00533-1 original sequence version: 191 DIP protein P00533 original sequence version: 218 DIP protein P00533 original sequence version: 221 DIP protein P00533-1 original sequence version: 221 DIP protein P00533 original sequence version: 226 DIP protein P00533 original sequence version: 166 DIP protein P00533-1 original sequence version: 166 DIP protein P00533 original sequence version: 194 DIP protein P00533-1 original sequence version: 194 DIP protein P00533-1 original sequence version: 224 DIP protein P00533 original sequence version: 189 DIP protein P00533-1 original sequence version: 207 DIP protein P00533 original sequence version: 177 DIP protein P00533 original sequence version: 168 D8A2A50B4EFB6ED2 prkdc-egfr 2 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 2A egfr-prkdc 2 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait P00533:p.[Arg669_Arg671delinsAlaAlaAla;Arg675Ala;Arg677Ala;Arg680_Arg681delinsAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain RRR AAA certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain RR AA certain certain sequence position certain certain certain sequence position certain R A Thu Jul 07 12:17:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg669,arg670,arg671,arg675,arg677,arg680,arg681_ala Fri Jul 22 11:14:51 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg669ala,arg670ala,arg671ala,arg675ala,arg677ala,arg680ala,arg681ala' Wed May 02 18:40:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg_arg669-671ala_ala_ala,arg675ala,arg677ala,arg_arg680-681ala_ala' Tue Jun 30 19:52:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg669_Arg671delinsAlaAlaAla;Arg675Ala;Arg677Ala;Arg680_Arg681delinsAlaAla]' P00533:p.Leu858Arg mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L R Wed May 02 18:18:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu858arg' Tue Jun 30 19:48:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu858Arg' P00533:p.[Arg669_Arg671delinsAlaAlaAla;Arg675Ala;Arg677Ala;Arg680_Arg681delinsAlaAla;Leu858Arg] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain L R certain certain sequence position certain certain certain sequence position certain RRR AAA certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain RR AA Thu Jul 07 12:16:23 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg669,arg670,arg671,arg675,arg677,arg680,arg681ala_leu858arg Fri Jul 22 11:04:25 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg669ala,arg670ala,arg671ala,arg675ala,arg677ala,arg680ala,arg681ala,leu858arg' Wed May 02 16:24:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg_arg669-671ala_ala_ala,arg675ala,arg677ala,arg_arg680-681ala_ala,leu858arg' Tue Jun 30 18:41:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg669_Arg671delinsAlaAlaAla;Arg675Ala;Arg677Ala;Arg680_Arg681delinsAlaAla;Leu858Arg]' physical association physical association 2B X rays, Cisplatin - a platinum containing anti-cancer drug which causes DNA cross linking. egfr-prkdc-1 3 5 unspecified role unspecified role neutral component neutral component 4 unspecified role unspecified role neutral component neutral component colocalization colocalization 3-6 Cisplatin - a platinum containing anti-cancer drug which causes DNA cross linking. Only cells transiently transfected with wtEGFR or the EGFRvIII mutant showed clear EGFR and DNA-PKcs nuclear expression/colocalisation after Cisplatin/IR treatment. Cells transfected with other EGFR mutants showed either reduced or absent nuclear accumulation of both EGFR and DNA-PKcs. there is lots of orange in the cytoplasm - but text infers that the interaction is only nuclear....(see PLA expt -quote "The experiments detailed earlier showed that nuclear translocation of EGFR is required for the association with DNA-PKcs.")- so have not entered any of the mutations, as it is not clear to me if there is any cytoplasmic interaction. egfr-prkdc-3 1 5 unspecified role unspecified role neutral component neutral component P00533:p.[Arg669_Arg671delinsAlaAlaAla;Arg675Ala;Arg677Ala;Arg680_Arg681delinsAlaAla] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain RR AA certain certain sequence position certain certain certain sequence position certain RRR AAA certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A Thu Jul 07 12:17:42 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was arg669,arg670,arg671,arg675,arg677,arg680,arg681_ala Fri Jul 22 11:10:07 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'arg669ala,arg670ala,arg671ala,arg675ala,arg677ala,arg680ala,arg681ala' Wed May 02 16:53:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg_arg669-671ala_ala_ala,arg675ala,arg677ala,arg_arg680-681ala_ala' Tue Jun 30 18:35:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg669_Arg671delinsAlaAlaAla;Arg675Ala;Arg677Ala;Arg680_Arg681delinsAlaAla]' 4 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 7 IR (4Gy, 20 mins) and Cisplatin - a platinum containing anti-cancer drug which causes DNA cross linking.