IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Phosphorylated nucleolin interacts with translationally controlled tumor protein during mitosis and with Oct4 during interphase in ES cells. Phosphorylated nucleolin interacts with translationally controlled tumor protein during mitosis and with Oct4 during interphase in ES cells. PLoS ONE 2010 imex curation Johansson H., Svensson F., Runnberg R., Simonsson T., Simonsson S. Affinomics - Interactions curated for the Affinomics consortium. Fig 2A-C - only the phospho -nucleolin form reacts, so have entered as a "required" phosphores. Figs 2 D,E-F only entered as "possible" as 2D only uses the phospho specific antibody used, and PLA (2E/F) shows some reaction of the unphospho form. stina.simonsson@gu.se 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Johansson H., Svensson F., Runnberg R., Simonsson T., Simonsson S. Affinomics - Interactions curated for the Affinomics consortium. Fig 2A-C - only the phospho -nucleolin form reacts, so have entered as a "required" phosphores. Figs 2 D,E-F only entered as "possible" as 2D only uses the phospho specific antibody used, and PLA (2E/F) shows some reaction of the unphospho form. Mitotic cells arrested in metaphase with demecolcine stina.simonsson@gu.se Accepted 2015-FEB-05 by PPORRAS PLoS ONE 2010 Only protein-protein interactions imex curation Phosphorylated nucleolin interacts with translationally controlled tumor protein during mitosis and with Oct4 during interphase in ES cells. Phosphorylated nucleolin interacts with translationally controlled tumor protein during mitosis and with Oct4 during interphase in ES cells. PLoS ONE 2010 imex curation Johansson H., Svensson F., Runnberg R., Simonsson T., Simonsson S. Affinomics - Interactions curated for the Affinomics consortium. Fig 2A-C - only the phospho -nucleolin form reacts, so have entered as a "required" phosphores. Figs 2 D,E-F only entered as "possible" as 2D only uses the phospho specific antibody used, and PLA (2E/F) shows some reaction of the unphospho form. stina.simonsson@gu.se 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions human-embryonic_stem_ cells Human-embryonic_stem_ cells (es cells) embryonic_stem_cell Embryonic stem cell (es cells) pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Johansson H., Svensson F., Runnberg R., Simonsson T., Simonsson S. Affinomics - Interactions curated for the Affinomics consortium. Fig 2A-C - only the phospho -nucleolin form reacts, so have entered as a "required" phosphores. Figs 2 D,E-F only entered as "possible" as 2D only uses the phospho specific antibody used, and PLA (2E/F) shows some reaction of the unphospho form. stina.simonsson@gu.se Accepted 2015-FEB-05 by PPORRAS PLoS ONE 2010 Only protein-protein interactions imex curation Phosphorylated nucleolin interacts with translationally controlled tumor protein during mitosis and with Oct4 during interphase in ES cells. Phosphorylated nucleolin interacts with translationally controlled tumor protein during mitosis and with Oct4 during interphase in ES cells. PLoS ONE 2010 imex curation Johansson H., Svensson F., Runnberg R., Simonsson T., Simonsson S. Affinomics - Interactions curated for the Affinomics consortium. Fig 2A-C - only the phospho -nucleolin form reacts, so have entered as a "required" phosphores. Figs 2 D,E-F only entered as "possible" as 2D only uses the phospho specific antibody used, and PLA (2E/F) shows some reaction of the unphospho form. stina.simonsson@gu.se 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Johansson H., Svensson F., Runnberg R., Simonsson T., Simonsson S. Affinomics - Interactions curated for the Affinomics consortium. Fig 2A-C - only the phospho -nucleolin form reacts, so have entered as a "required" phosphores. Figs 2 D,E-F only entered as "possible" as 2D only uses the phospho specific antibody used, and PLA (2E/F) shows some reaction of the unphospho form. Mitotic extracts were prepared by arresting ES cells in metaphase by adding Demecolcine. stina.simonsson@gu.se Accepted 2015-FEB-05 by PPORRAS PLoS ONE 2010 Only protein-protein interactions imex curation Phosphorylated nucleolin interacts with translationally controlled tumor protein during mitosis and with Oct4 during interphase in ES cells. Phosphorylated nucleolin interacts with translationally controlled tumor protein during mitosis and with Oct4 during interphase in ES cells. PLoS ONE 2010 imex curation Johansson H., Svensson F., Runnberg R., Simonsson T., Simonsson S. Affinomics - Interactions curated for the Affinomics consortium. Fig 2A-C - only the phospho -nucleolin form reacts, so have entered as a "required" phosphores. Figs 2 D,E-F only entered as "possible" as 2D only uses the phospho specific antibody used, and PLA (2E/F) shows some reaction of the unphospho form. stina.simonsson@gu.se 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Johansson H., Svensson F., Runnberg R., Simonsson T., Simonsson S. Affinomics - Interactions curated for the Affinomics consortium. Several proteins other than those entered here were found on the gel, but the only identifications given are ambiguous; notably:- Myosin heavy polypeptide 9. Actin - cytoplasmic type 8, gamma-actin, Ribosomal protein L10, Tropomyosin. Fig 2A-C - only the phospho -nucleolin form reacts, so have entered as a "required" phosphores. Figs 2 D,E-F only entered as "possible" as 2D only uses the phospho specific antibody used, and PLA (2E/F) shows some reaction of the unphospho form. stina.simonsson@gu.se Accepted 2015-FEB-05 by PPORRAS PLoS ONE 2010 Only protein-protein interactions imex curation Phosphorylated nucleolin interacts with translationally controlled tumor protein during mitosis and with Oct4 during interphase in ES cells. Phosphorylated nucleolin interacts with translationally controlled tumor protein during mitosis and with Oct4 during interphase in ES cells. PLoS ONE 2010 imex curation Johansson H., Svensson F., Runnberg R., Simonsson T., Simonsson S. Affinomics - Interactions curated for the Affinomics consortium. Fig 2A-C - only the phospho -nucleolin form reacts, so have entered as a "required" phosphores. Figs 2 D,E-F only entered as "possible" as 2D only uses the phospho specific antibody used, and PLA (2E/F) shows some reaction of the unphospho form. stina.simonsson@gu.se 26-Feb-2015: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Johansson H., Svensson F., Runnberg R., Simonsson T., Simonsson S. Affinomics - Interactions curated for the Affinomics consortium. Fig 2A-C - only the phospho -nucleolin form reacts, so have entered as a "required" phosphores. Figs 2 D,E-F only entered as "possible" as 2D only uses the phospho specific antibody used, and PLA (2E/F) shows some reaction of the unphospho form. stina.simonsson@gu.se Accepted 2015-FEB-05 by PPORRAS PLoS ONE 2010 Only protein-protein interactions imex curation po5f1_mouse POU domain, class 5, transcription factor 1 Pou5f1 Oct-3 Oct-4 Otf-3 Otf3 Octamer-binding transcription factor 3 NF-A3 Octamer-binding protein 3 Octamer-binding protein 4 protein protein mouse Mus musculus Mouse MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPPGGPGIGPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEGTSSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQARKRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRSSIEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSVPVTALGSPMHSN DIP protein P20263 original sequence version: 87 DIP protein P20263 original sequence version: 121 DIP protein P20263 original sequence version: 118 DIP protein P20263 original sequence version: 137 DIP protein P20263 original sequence version: 107 757E41DF52286714 h13_mouse Histone H1.3 H1-3 H1 VAR.4 H1d H1f3 Hist1h1d protein protein mouse Mus musculus Mouse MSETAPAAPAAPAPVEKTPVKKKAKKTGAAAGKRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKKAGAAKAKKPAGAAKKPKKATGAATPKKTAKKTPKKAKKPAAAAGAKKVSKSPKKVKAAKPKKAAKSPAKAKAPKAKASKPKASKPKATKAKKAAPRKK 19D888981281E55C rl8_mouse 60S ribosomal protein L8 Rpl8 protein protein mouse Mus musculus Mouse MGRVIRGQRKGAGSVFRAHVKHRKGAARLRAVDFAERHGYIKGIVKDIIHDPGRGAPLAKVVFRDPYRFKKRTELFIAAEGIHTGQFVYCGKKAQLNIGNVLPVGTMPEGTIVCCLEEKPGDRGKLARASGNYATVISHNPETKKTRVKLPSGSKKVISSANRAVVGVVAGGGRIDKPILKAGRAYHKYKAKRNCWPRVRGVAMNPVEHPFGGGNHQHIGKPSTIRRDAPAGRKVGLIAARRTGRLRGTKTVQEKEN CE1610449749318B nucl_mouse Nucleolin Protein C23 Ncl Nuc protein protein mouse Mus musculus Mouse MVKLAKAGKTHGEAKKMAPPPKEVEEDSEDEEMSEDEDDSSGEEEVVIPQKKGKKATTTPAKKVVVSQTKKAAVPTPAKKAAVTPGKKAVATPAKKNITPAKVIPTPGKKGAAQAKALVPTPGKKGAATPAKGAKNGKNAKKEDSDEDEDEEDEDDSDEDEDDEEEDEFEPPIVKGVKPAKAAPAAPASEDEEDDEDEDDEEDDDEEEEDDSEEEVMEITTAKGKKTPAKVVPMKAKSVAEEEDDEEEDEDDEDEDDEEEDDEDDDEEEEEEEPVKAAPGKRKKEMTKQKEAPEAKKQKVEGSEPTTPFNLFIGNLNPNKSVNELKFAISELFAKNDLAVVDVRTGTNRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGRDSKKVRAARTLLAKNLSFNITEDELKEVFEDAMEIRLVSQDGKSKGIAYIEFKSEADAEKNLEEKQGAEIDGRSVSLYYTGEKGQRQERTGKTSTWSGESKTLVLSNLSYSATKETLEEVFEKATFIKVPQNPHGKPKGYAFIEFASFEDAKEALNSCNKMEIEGRTIRLELQGSNSRSQPSKTLFVKGLSEDTTEETLKESFEGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDFKPQGKKTKFE EE2CE2ACDBF54CD4 npm_human Nucleophosmin NPM1 NPM Nucleolar phosphoprotein B23 Numatrin Nucleolar protein NO38 protein protein human Homo sapiens Human MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEEEEDVKLLSISGKRSAPGGGSKVPQKKVKLAADEDDDDDDEEDDDEDDDDDDFDDEEAEEKAPVKKSIRDTPAKNAQKSNQNGKDSKPSSTPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL 620BC7BA2E4A0054 h14_mouse Histone H1.4 H1-4 H1 VAR.2 H1e H1f4 Hist1h1e protein protein mouse Mus musculus Mouse MSETAPAAPAAPAPAEKTPVKKKARKAAGGAKRKTSGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKRAGAAKAKKPAGAAKKPKKAAGTATAKKSTKKTPKKAKKPAAAAGAKKAKSPKKAKATKAKKAPKSPAKAKTVKPKAAKPKTSKPKAAKPKKTAAKKK 9463467F699F3625 tctp_mouse Translationally-controlled tumor protein Tpt1 Trt p23 21 kDa polypeptide p21 protein protein mouse Mus musculus Mouse MIIYRDLISHDELFSDIYKIREIADGLCLEVEGKMVSRTEGAIDDSLIGGNASAEGPEGEGTESTVVTGVDIVMNHHLQETSFTKEAYKKYIKDYMKSLKGKLEEQKPERVKPFMTGAAEQIKHILANFNNYQFFIGENMNPDGMVALLDYREDGVTPFMIFFKDGLEMEKC 7F46751DA2D281D2 nucl_human Nucleolin NCL Protein C23 protein protein human Homo sapiens Human MVKLAKAGKNQGDPKKMAPPPKEVEEDSEDEEMSEDEEDDSSGEEVVIPQKKGKKAAATSAKKVVVSPTKKVAVATPAKKAAVTPGKKAAATPAKKTVTPAKAVTTPGKKGATPGKALVATPGKKGAAIPAKGAKNGKNAKKEDSDEEEDDDSEEDEEDDEDEDEDEDEIEPAAMKAAAAAPASEDEDDEDDEDDEDDDDDEEDDSEEEAMETTPAKGKKAAKVVPVKAKNVAEDEDEEEDDEDEDDDDDEDDEDDDDEDDEEEEEEEEEEPVKEAPGKRKKEMAKQKAAPEAKKQKVEGTEPTTAFNLFVGNLNFNKSAPELKTGISDVFAKNDLAVVDVRIGMTRKFGYVDFESAEDLEKALELTGLKVFGNEIKLEKPKGKDSKKERDARTLLAKNLPYKVTQDELKEVFEDAAEIRLVSKDGKSKGIAYIEFKTEADAEKTFEEKQGTEIDGRSISLYYTGEKGQNQDYRGGKNSTWSGESKTLVLSNLSYSATEETLQEVFEKATFIKVPQNQNGKSKGYAFIEFASFEDAKEALNSCNKREIEGRAIRLELQGPRGSPNARSQPSKTLFVKGLSEDTTEETLKESFDGSVRARIVTDRETGSSKGFGFVDFNSEEDAKAAKEAMEDGEIDGNKVTLDWAKPKGEGGFGGRGGGRGGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE DIP protein P19338 original sequence version: 93 DIP protein P19338 original sequence version: 123 C97F6E34E5CA6727 h11_mouse Histone H1.1 H1-1 H1 VAR.3 H1a H1f1 Hist1h1a Histone H1a protein protein mouse Mus musculus Mouse MSETAPVAQAASTATEKPAAAKKTKKPAKAAAPRKKPAGPSVSELIVQAVSSSKERSGVSLAALKKSLAAAGYDVEKNNSRIKLGLKSLVNKGTLVQTKGTGAAGSFKLNKKAESKAITTKVSVKAKASGAAKKPKKTAGAAAKKTVKTPKKPKKPAVSKKTSKSPKKPKVVKAKKVAKSPAKAKAVKPKASKAKVTKPKTPAKPKKAAPKKK Histones H1 are necessary for the condensation of nucleosome chains into higher order structures mint 03A46320A100B203 po5f1_human POU domain, class 5, transcription factor 1 Octamer-binding transcription factor 3 Octamer-binding protein 3 POU5F1 OCT3 OCT4 OTF3 Octamer-binding protein 4 protein protein human Homo sapiens Human MAGHLASDFAFSPPPGGGGDGPGGPEPGWVDPRTWLSFQGPPGGPGIGPGVGPGSEVWGIPPCPPPYEFCGGMAYCGPQVGVGLVPQGGLETSQPEGEAGVGVESNSDGASPEPCTVTPGAVKLEKEKLEQNPEESQDIKALQKELEQFAKLLKQKRITLGYTQADVGLTLGVLFGKVFSQTTICRFEALQLSFKNMCKLRPLLQKWVEEADNNENLQEICKAETLVQARKRKRTSIENRVRGNLENLFLQCPKPTLQQISHIAQQLGLEKDVVRVWFCNRRQKGKRSSSDYAQREDFEAAGSPFSGGPVSFPLAPGPHFGTPGYGSPHFTALYSSVPFPEGEAFPPVSVTTLGSPMHSN DIP protein Q01860 original sequence version: 131 DIP protein Q01860 original sequence version: 150 DIP protein Q01860 original sequence version: 138 934C58DAEA0C535B h15_mouse Histone H1.5 H1-5 H1 VAR.5 H1b H1f5 Hist1h1b protein protein mouse Mus musculus Mouse MSETAPAETAAPAPVEKSPAKKKTTKKAGAAKRKATGPPVSELITKAVSASKERGGVSLPALKKALAAGGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKKTGAAKAKKPAGATPKKPKKTAGAKKTVKKTPKKAKKPAAAGVKKVAKSPKKAKAAAKPKKAAKSPAKPKAVKSKASKPKVTKPKTAKPKAAKAKKAVSKKK B9C26AC31C2716B6 actb_mouse Actin, cytoplasmic 1 Actb Beta-actin protein protein mouse Mus musculus Mouse MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF 6AFD05CA94E360E2 npm_mouse Nucleophosmin Nucleolar phosphoprotein B23 Numatrin Nucleolar protein NO38 Npm1 protein protein mouse Mus musculus Mouse MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEDEEDVKLLGMSGKRSAPGGGNKVPQKKVKLDEDDEDDDEDDEDDEDDDDDDFDEEETEEKVPVKKSVRDTPAKNAQKSNQNGKDLKPSTPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL E68750C549ED25E6 tpt1-actb 4 14 unspecified role unspecified role prey prey mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells 17 unspecified role unspecified role prey prey mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells 8 unspecified role unspecified role prey prey mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells 16 unspecified role unspecified role prey prey mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells 11 unspecified role unspecified role prey prey mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells 7 unspecified role unspecified role prey prey mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells 9 unspecified role unspecified role prey prey mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells 12 unspecified role unspecified role bait bait n_terminus s tag s tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) association association Figure 1 Several other proteins are stated to have been found on the gel, but have been ambiguously identified, notably:- Myosin heavy polypeptide 9. Actin - cytoplasmic type 8, gamma-actin, Ribosomal protein L10, Tropomyosin. ncl-tpt1 5 12 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component required_phosphores phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 2 B/C Demecolcine (colcemid) It depolymerises microtubules and inactivates spindle fibre formation, thus arresting cells in metaphase. Substantially more colocalization is visualized with phosphorylated Nucleolin and Tpt1 (Figure 2B–C) as compared to non- phosphorylated Nucleolin and Tpt1.(Fig 2A) tpt1-ncl 3 12 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey possible_phosphores phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Co-immunoprecipitation of Ncl-P was detected in the presence of 1m NaCl, but not in the presence of 0.1M citrate. 2 D ncl-tpt1-2 1 9 unspecified role unspecified role neutral component neutral component possible_phosphores phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 2 E/F ncl-tpt1-3 5 6 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component required_phosphores phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 3A, Table 1 ncl-pou5f1 1 9 unspecified role unspecified role neutral component neutral component possible_phosphores phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 3 B/C ncl-pou5f1-1 2 15 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 3 D/E ncl-pou5f1-2 2 10 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction Supp 2B Positive control ncl-npm1 1 18 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction Supp 2A Positive control