IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ emi-2005-1 Isolation of a protein interacting with Vfphot1a in guard cells of Vicia faba. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Emi T., Kinoshita T., Sakamoto K., Mineyuki Y., Shimazaki K. kenrcb@mbox.nc.kyushu-u.ac.jp Vicia faba guard cell cDNA library Plant Physiol. (0032-0889) 2005 Only protein-protein interactions imex curation emi-2005-2 Isolation of a protein interacting with Vfphot1a in guard cells of Vicia faba. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Emi T., Kinoshita T., Sakamoto K., Mineyuki Y., Shimazaki K. A directed yeast two hybrid was used. kenrcb@mbox.nc.kyushu-u.ac.jp Vicia faba guard cell cDNA library Plant Physiol. (0032-0889) 2005 Only protein-protein interactions imex curation emi-2005-3 Isolation of a protein interacting with Vfphot1a in guard cells of Vicia faba. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Emi T., Kinoshita T., Sakamoto K., Mineyuki Y., Shimazaki K. A microsomal fraction from etiolated seedlings of V.faba or seedlings illuminated with blue light (for 1 minute) were used in this study. kenrcb@mbox.nc.kyushu-u.ac.jp Vicia faba guard cell cDNA library Plant Physiol. (0032-0889) 2005 Only protein-protein interactions imex curation emi-2005-4 Isolation of a protein interacting with Vfphot1a in guard cells of Vicia faba. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Emi T., Kinoshita T., Sakamoto K., Mineyuki Y., Shimazaki K. kenrcb@mbox.nc.kyushu-u.ac.jp Vicia faba guard cell cDNA library Plant Physiol. (0032-0889) 2005 Only protein-protein interactions imex curation q8h935_vicfa Vfphot1a protein protein vicfa Vicia faba (Broad bean) Broad bean Faba vulgaris MEPFTRDHRGSLEVFNPSSSETNGTPNPNPNPNPSNSWNTGTSSRGTEAPPLRDSIISDEVPTATSWMALKETTPSPKSGESGSVAEQRAAEWGLVLKTDSETGKPQGVGVRGSGGGGGSRRDSNNSVRSSGESSDDGREGGRGIPRVSEDLRDALSAFQQTFVVSDATKPDYPIMYASAGFFSMTGYTSKEVIGRNCRFMQGADTDPNDVAKIREALAAGTSYCGRLLNYKKDGTTFWNLLTIAPIKDEHGKILKLIGMQVEVSKHTEGTKEKMLRPNGLPESLIRYDARQKEKANSSVTELVEAVSKRPRSLSESANRLPFNKKPTNGSNDHATPPNSESSSRKSGSTLRSFRRKSHSGAGNSNSMHPITELPENNNKSRRRSFMGFMRKSLSNNERFNHEQVIDRNSSEDEDRLDSFDEQNIAQKREKRKGFDLATTLERIEKNFVITDPRLPDNPIIFASDSFLELTEYSREEILGRNCRFLQGPETDPATVKKIRYAIDNQTEVTVQLINYTKTGKKFWNLFHLQPMRDQKGEVQYFIGVQLDGSQHVEPLHNGIAEDTAKEGENLVKKTAENVDDALRELPDANMKPEDLWMNHSKVVHPKPHRREDSAWRAIQKIMESGEQIGLKHFKPIKPLGSGDTGSVHLVELCGTDHHFAMKAMDKGVMPNRNKVHRACTEREILDMLDHPFLPALYASFQTKTHICLITDYCPGGELFMLLDRQPAKVLKEDAVRFYATEVVVALEYLHCQGIIYRDLKPENVLLQSTGHVSLTDFDLSCLTSCKPELIVPSTNDKKKGQHGPIFMAEPMRASNSFVGTEEYIAPEIITGSGHTCAVDWWALGILLYEMFYGYTPFRGKNRQRTFANILHKDLKLPKSKQVSLSAKQLIYHLLQRDPTSRLGSKGGANDIKHHSFFKGINWALVRCTKPPELDAPLFDTNKEEKEKDDKYVDNGQEDMSVF q4w5w8_vicfa vfPIP protein protein vicfa Vicia faba (Broad bean) Broad bean Faba vulgaris MAHRSSRRRNTAPPESTPPTAPAAPTIPAKQPRPTIFSPSPSHYTKANSDLIQATKSSFLVKPFQSLNLGSKKTKHSQTKHVDSRLHTKSMTTSAIFESSKPNKTHNTKNIVLTPKLEKESIFLTSKKTHKEKPLKPGSELERWKLQGLLKNEKKESCKGCVVEDVKDEKSRKLAVVEEGDVVKERVSVSMGQSGGGGGGGRRKSLCGSMVDLGDFFAINGAKMVSADMPPFMQIHAVDCARKAFDSMEKFTSKTLASSLKKEFDGVYGPAWHCIVGTSFGSFVTHSVGGFLYFSMDQKLYILLFKTAVQKAD vfphot1a-vfpip Yeast two hybrid analysis reveals interaction between Vfpho1a and VfPIP protein 491282 N/A 491286 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 491287 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range false physical association physical association false false false The region used for the bait Vfphot1a was not stated in the text. The coordinates used are those for the Vfphot1a protein without the C terminal kinase domain. vfphot1a-vfpip-1 Directed yeast two hybrid analysis reveals interaction between Vfpho1a and VfPIP protein 491283 N/A 491286 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 491287 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figure 4 C. vfpip-vfphot1a GST pulldown analysis reveals interaction between Vfpho1a and VfPIP protein 491284 N/A 491286 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false c terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 491287 unspecified role unspecified role prey prey vicfa Vicia faba (Broad bean) Broad bean Faba vulgaris physical association physical association false false false Figure 2 A. Vfpho1a from microsomal fractions of etiolated and blue light treated seedlings demonstrated interaction with VfPIP. The authors present data for Vfpho1a interaction with 14-3-3 protein using the same techniques but do not identify which 14-3-3 protein was used in the study. Interaction between the two proteins was only observed when seedlings were treated for 1 minute with blue light. vfphot1a-vfpip-2 Far western analysis reveals interaction between Vfpho1a and VfPIP protein 491285 N/A 491286 unspecified role unspecified role prey prey c terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 491287 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Figure 3 A. MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint salomon-2004-2 Dimerization of the plant photoreceptor phototropin is probably mediated by the LOV1 domain. in vitro In vitro experimental interac experimental interaction detection experimental interac predetermined predetermined participant predetermined Salomon M., Lempert U., Rüdiger W. FEBS Lett. (0014-5793) 2004 Only protein-protein interactions imex curation q8h934_vicfa Vfphot1b protein protein vicfa Vicia faba (Broad bean) Broad bean Faba vulgaris MEKHLKKSPSSSSSSSMRPSFPRDPRGSLEVFNPTSNTSSPVRSPSNLKNWTETEEPRNEFPDKVTNTSWMAIKEGETGAAVQRAAEWGLVLTTDAETGKPQGVAVRHSGGDEPNAVELESKRNSNNTVRTSGESSDGGDPRGFPRVSDDLKDALSAFQQTFVVSDATKPDYPILYASAGFFKMTGYTSKEVIGRNCRFLQGADTDPNDVARIREALEGGKSFCGRLLNYKKDGTPFWNLLTISPIKDDDGNVLKLIGMLVEVNKHTEGSKEKKLRPNGLPESLIRYDARQKEKATSSVSELLEAMKRPRAMSESGHRPFIRKSGGGGSSEEDERLENKSRRKSDSVASFRPKPQGKIRHSMERISELPENKQKNSRRGSFMGFMRKSHSIDESIDNEVIVDVSSGSEDDERDDSFEFDDKEKLKEKRKGLDLATTLERIEKNFVITDPRLPDNPIIFASDSFLELTEYSREEILGKNCRFLQGQETDPATVRKIREAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDHKGEVQYFIGVQLDGSQHVEPLHNCIAEESAKEGELLVKETAENVGEAVKELPDANQKPDDLWKNHSKVVRPKPHRKDDDAWRAIQNVVGNGEQVGLKHFRPIKPLGSGDTGSVHLVELEGTGHYFAMKAMDKGVMLNRNKVHRACTEREILDMLDHPFLPALYASFQTKTHVCLITDYYPGGELFLLLDQQPTKVLKEDSVRFYAAEVVIALEYLHCLGIIYRDLKPENVLIQRNGHVSLTDFDLSCLTSCKPQLILPATEEKKKRKNKKKKGQPKNQEVPMFMAEPMRASNSFVGTEEYIAPEIITGSGHTSAVDWWALGILLYEMLYGYTPFRGKTRQKTFGNILHKDLKFPKSKPVSPHGKQLIYWLLHRDPKNRLGSLEGANEIKNHPFFKNVNWALVRCMKPPELDAPILLDNDEKKEAKDIDPGLDDLQKNIF mint vfphot1b-vfphot1b 165620 q8h934_vicfa 165622 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role unspecified role unspecified role purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 q8h934_vicfa 165622 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role unspecified role unspecified role purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 association association false false false F3 mint