DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hashem-2013-1 trybb Trypanosoma brucei brucei 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Hashem Y., des Georges A., Fu J., Buss SN., Jossinet F., Jobe A., Zhang Q., Liao HY., Grassucci RA., Bajaj C., Westhof E., Madison-Antenucci S., Frank J. Ribosomal RNA, which is required for the interaction to happen, was also present in this structure, but it was not captured in this entry. jf2192@columbia.edu Nature 2013 imex curation Only protein-protein interactions imex curation q381j6_tryb2 60S ribosomal protein L27 Tb11.01.7535 Tb11.01.7545 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MKFLKPGKVVIMTSGRYAGKKAVIVQNTDTRNKERPYGHSLLAGIKKYPRKVVRGMSKRSITRRSQVGVFLRVVNHKHFLPTRYNMDLSRELRGRINVSDASKKAKSKQLVKRLFQARYNAGNNRWFFQRLRF q38d25_tryb2 Tb09.211.4850 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVGIKCRNRRKARRAHFQAPSHVRRILMSAPLSKELRAKYNVRSMPVRKDDEVRVKRGKFKGREGRVTACYRLKWVIHIDKVSCEKANGTTVPVGVHTSNVEITKLKLNTRRKAILERKDRSTKTDKSKGKVTAAEKAMQQMD q38b56_tryb2 Tb10.70.1540 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MRTIDCEFSHFAVHPGHGRRYVPFAFLSTKPVLTFARPKCFAMYMRKKNPRFIAWTRTYRRIHRKTTTDRVGRRRAARTVRAERAIVGAELSYIQEVRAKAKKVDRTAKGKAVREEMAARKAAKK q57zh2_tryb2 Tb927.7.5170 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MPAKAASAAASKKNSAPKSAVSKKVAKKGAPAAAAKPTKVVKVTKRKAYTRPQFRRPHTYRRPATVKPSSNVSAIKNKWDAFRIIRYPLTTDKAMKKIEENNTLTFIVDSRANKTEIKKAIRKLYQVKTVKVNTLIRPDGLKKAYIRLSASYDALDTANKMGLV q57w56_tryb2 Tb11.01.7960 Tb927.5.1110 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MGKTVLTCRKGNGSVYQVHGHKRLGPAKLRILDYAERHGFMRGVVKAIEHEPGRGAPLARVEFRHPYKYRRVKELMVAPEGMFTGQSMLCGVKAPLAIGNVLPLGQITEGCIVCNVEAKVGDRGTLARASGDYCIIISHNTETGRTRLKLPSGQKKTVPSSCRAMIGIVAGGGRIEKPVLKAGNSFYRFRGKRNCWPKVRGVARNPVEHPHGGGNHQHIGHPSTVSRHAPPGQKVGLIAARRTGRIRGSRAVRGAWHPEE q383s6_tryb2 60S ribosomal protein L29 Tb11.01.1790 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MAKSKNHTNHNQSRKNHRNGIKPPLPLYMYNSKRGGWLPALVNTRRVRKNNQKAALKARRERLAAHQAAQK q385e9_tryb2 Tb11.02.4050 Tb11.02.4070 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTHSTDLQWLLVRQNSKFLQKRNGIRLSSDPFNNNANWTKRQSGFLNTKAAVIKTKGDRILLTTKSGDTNNKPKLMYKKTVMEPGVKSSVVKRAVADIRPDLAKMAYRRARKMACTITRMKKVCAARKERSSKMHFHRKTVRPKRN q38dn9_tryb2 Tb09.211.2650 Tb09.v1.0640 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MPTRFKKTRHQRGSTFCGYGRVGKHRKHPSGRGNAGGEHHHRINFRKYHPGYFGKCGMNHYHKKKNTTWKPTINLDNLTKLMAKDEAMKAKKGEALPVIDLLANGYSKLLGNGHLQAPCIVKARWVSKLADKKIRKAGGAVVLQA q38cl1_tryb2 Tb10.100.0155 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVKPFVPRNIVKKRTKKFTRHRCELFPQLSSSWRKPRGEDSPVRRRYKGQKAMPNKGYGSDRRTKYITPSGFKNFPVNNVQDLYMLLMQNRKYAGVISHTVGAKARKAIVRKAHELDVRLINGNAKLRRVDV q38dh8_tryb2 Tb09.211.3270 Tb09.211.3280 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTRAGFKGKVLGKEKKLALLEARKKAAEARKSRDDRRWKRVLAKMDPEKRKKYHGVGNTAEHSRVRGCTRASLFKRTGRKPDNIVMEASIHLSKLLKKRTFHKRAPIAIKRIRSFVGKLMKTKDNRIDASLNTFIWHKGVKGVPGRVRVRVERKSETMEGGKRKHFYTVISHIPVPSFKNLTTKVIEQ q38bi9_tryb2 Tb10.70.3170 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MAKKVKSKVDTINTKIQLVMKSGKYVLGTQQSLKTLRQGRSKLVVISANCPPIRKAEIEYYCTLSKTPIHHYSGNNLDLGTACGRHFRACVLSITDVGDSDITSA q581q1_tryb2 Tb927.4.1800 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSHCKFEHPRHGHLGFLPRKRSRQIRGRARSFPKDDPSQKPHLTSFMVYKAGMTHIVRDVDRPGSKVNKKEVVEPVTILEAPPMVVVGIVGYRQTPVGHKTIGTVWAHHTSVEFRRRFYKNWKQSAQLAFTKRKQFARTTEGRLAEARTLKAFEKKADIIRVVAHTQLRKLRNNRVGVKKAHVSEIQINGGTIAQKIELAKSLLEKEVRIDSVFQQSEACDVCAVTKGHGFTGVVKRWGVACLPRKTHRGLRKVACIGAWHPSRVMYTVARAGQHGYHHRTHLNKKIYQLGRAVSMEPNQATTAYDLTAKTITPMGGFVGYGTVRNDYVMLKGSVAGPRRRVITLRRPMAPQTSRKLTEKITLKFIDTSSKIGHGRFQTKKEKSQWYGPLKKDRIRREERLRKERAARAAERKAKGGATTTAAPRKSKK q38cy6_tryb2 Tb09.244.2725 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MAATKREAPVPRTGIIAGFNKGYKTTRRARRPSSNDRYALPHKKLRAVKAIISDLVGFSPMERRVQELLRVGKDKRALKFCKKRLGSIKAAKKRRAKVEEALRQQTKKK q584g9_tryb2 Tb927.4.2180 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MALVKKFSGSVAANRSKKIHQLTKKTTRVKRDRKSPRLYMKGTLAGYTRGLHGQNKNTALVRIENVNTTEDAKWYVGKRVCYVYHGYKIKRCVRWSKAPARRSNTRAIWGRITRPHGTSGTVRVKFNGSSVPASAIGRRIRVYLYPSRI q38fw8_tryb2 Tb09.160.0700 Tb09.160.0710 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSHIVKIRDLKEKGKDDLLKQLSEFKKELSQLRVSQQMNVGAARLGRIRTIRKGIARIMTVLNKNERENLRKFYSDKKLRSAKPKTLRAKLTHRRRLALKANEKNRKTRRQLRMAHKFPRRIYAVKV q383v6_tryb2 Tb11.01.1480 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSCPRVQYRRRMHYATRGNRMRLVRTPGNRLVMQKRGKRSQGPHTPWVLGHKRLAGTKALRHTKARLAPRHQKTTSRPYGGVLSHEQVRDRIVRAFLIEEQRIVKRALKAHAKVQKEKKRRAAKRKSKEEKVAAVAKKVAAKVGAKSVIAKKEAPKRKAGKAPVGAKLKK q57uj8_tryb2 Tb927.8.5260 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MGRFKPLAIKKKYAKKLKQNRPVPYWIRLRTGNRIKWNEKRRHWRRTKLHY q38fv6_tryb2 Tb09.160.0815 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MPREIKNLKEFLAICSRKDARCVKVKHNPKVTKFKVRCSRHLYTLVMADKKKADKIERSIHPSVKKITVTSRSHANKNTPSK q38aw9_tryb2 Tb10.100.0110 Tb10.70.0465 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MAKRTVKMGVMGRYGARYGSNPRKRAKKLEVSQHAKHFCSFCGKFAFRRKAVGIWRCDGCSKTVAGGAYTLSTPNNTTVRSTVRRLRELKQSN q386l1_tryb2 Ribosomal protein L37 Tb09.211.0865 Tb11.02.0500 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTKGTTSMGQRHGRTHILCRRCGRNAYHVQWERCAACAYPRAQRRRYNWSVKAIKRRRTGTGRCRYLKVVHRRIRNHFKTDIKA q38cy8_tryb2 Tb09.244.2730 Tb09.244.2740 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTFVKVVKNKAYYKRFQVKYRRRREGKTDYHARRRMVLQDKTKFGTPKYRLVVRITNRDVIAQIVHAKVVGDEVVMAAYSHELPLFGIEHGLTNYAAAYATGLLVARRMLAKLGLAEKFVGVKEVDGSYAAVRTKDDDQGDDESRFPFKAILDVGLARTTTGARVFGVLKGAVDGGLAVPHRPNRFPGYNKESDALNAKVHRDRIFGRHVADYLKQVREEASSNPDEKTCQFSKFIAAKVSPDDMEGMYKRAHAAIRADPTKRRAKKERPAEAKPKKYNTVKLTGAEKKAAAKAKVAAVIERIRDRAK d7sg81_tryb2 Tb11.02.0740 Tb927.2.6090 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVNYPKKKKMHCPDERCNAHKSFKVVQYKAGKARLYARGKRRYDRKQSGYGGQTKPIFHKKAKTTKKIVLKLQCSNCKSIIQNVLKRTKHFELNDKKKTGNKDPTW rl40_trybb Ubiquitin-60S ribosomal protein L40 protein protein trybb Trypanosoma brucei brucei MQIFVKTLTGKTIALEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEEGRTLADYNIQKESTLHLVLRLRGGVMEPTLEALAKKYNWEKKVCRRCYARLPVRATNCRKKGCGHCSNLRMKKKLR q582n2_tryb2 Tb927.3.5050 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTARPSVSVYSASEDKVVGTCSLPAVFTAPIRHDVVQFVHTNMAKNSRQPYAVNRLSGMKHSAESWGTGRAVARIPRIHGGGTSMSGAGAFGNMCRGGRMFAPTKIFRRWHRKINLHQKRFAVVSALAASSLPALVMSRGHKIENVAEVPLVVEDGVRAYEKTKEAMTFLKTVGAIDDVNRVNDSRQIRAGRGKMRNRRYVARRGPMLVMPDNKGTRAFRNIFGLDLANVNSLNLLHLAPGGHVGRFIIWTKSAFEKLDKIFGTFTEPSTVKSGFMLPAPMLTSTDVTRIMQSEEVRRVLKPKKLQPKRPSRYRQPTNGIRNRRLRLRLNPFQKKEKAMAKGMQNKKNREARHAAKVVRLAKARKNVAKALKKK q38cd0_tryb2 Tb10.70.7010 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MKIKSHDQITFPEDVTVSVKDRIVTVKGKRGTLTKDLRHLQLDFRVNKKLRTFTAVRWFGNKINNSTINTALSHVRNMITGVTKGFRFKVRFAYAHFPISVTVENQLVEIRNFLGEKRVRRQVVADDVKVYRTDAALVKDELVLEGNDLEQVSREAAVMHQLCLVKKKDIRKFLDGIYVQTKTNIEVDE q57vm1_tryb2 60S ribosomal protein L7a Tb927.8.1330 Tb927.8.1340 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MAGKEVKKAVKPTKKAGVPYKKPEVTQKKAKASAAAPSPFVARPKDFGIGRDVPYARDLSRFMRWPTFVTMQRKKRVLQRRLKVPPALNQFTKVLDRSSRNELLKLVKKYAPETRKARRDRLTKVAEEKKKNPKGTVSTKAPLCVVSGLQEVTRTIEKKTARLVLIANNVDPIELVLWMPTLCRANKIPYAIVKDKARLGDAIGRKTATCVAFTDVNAEDQAALKNLTRSVNARFLARSDVIRRQWGGLQLSLRSRAELRKKRARTAGNDAAAKAA q57v35_tryb2 Tb927.7.1740 Tb927.7.1750 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MFMTGLILLGKGREVMPAKAVPAPESAIKRAAFKQQQTENFKKAIAANKVAAAALKKLAYSRGLKYSREYRSTEKKLAGLRRNARQHGNYYLEAKPKVAVVTRIRGIAKVPPKQRKILQLLRLRQIFNTVFVRLNKPMENMLRAVEPYIAYGYPSLSTVRAMVYKRGHLKINGQRVKITDNQMIKDKYHNDDIVCAEDIVNQIYACGKHFRTVTNGLWPFKLAPPAGGMRQKRRHFVEGGDYGNRDTLINRFLARMI q389k1_tryb2 Tb10.26.0560 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MPAATKKAVEKKAKKVSRKSPEYSTVRKSCKPGTIAIILAGRFRGRRAVILKQLPKNGPLVISGPMKYSGVPIRRIDSRYIIATSTRVDLTGVDTSAITPEIFKREKKEKRVKSEGEFMGDKDKKKAESKAKKTSKAAPKGTVSDERAQLQNAIDTALIQAIKKDPLGKEMAGYLHSVFTIKPGDAPHRLKW q386f2_tryb2 40S ribosomal protein S4 Tb11.02.1085 Tb11.02.1090 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MAKKHLKRLYAPKDWMLSKLTGVFAPRPRAGPHKLRECLSLLIIIRNRLKYALNALEAQMILRQGLVCVDGKPRKDGKYPAGFMDVVEIPKTGDRFRILYDVKGRFALVRVSEAESSIKMMKVVNVYTGTGRIPVAVTHDGHRIRYPDPRTSRGDTLVYDVKEKKVLDLIKIGNGKVVMVTGGANRGRIGEIVSIERHPGAFDIARLKDASGHEFATRATNIFVIGKDMSSVPVTLPKQQGLRINVIQEREEKLIAAETRRTTQAHSKRKTKV q38bk6_tryb2 40S ribosomal protein S3a Tb10.70.3360 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTLGKNKRISKGGKRGKKKAQEAMSRKEWYDVVAPKNFEVRQFAKTICNKTQGTKIAADFLRGRVYEGNLADLNKTQNEDDAYRKVKFTVQEVQGRNLLTQFHGMDMTSDRVYYLLRKWCTTIEATVEAKTADGYGLRLFLIAFTKKQENQLSKNCYAKTRLVKWVRMRATNIIRRRLAKLDINDAVSLLTRNILRDRLAKRCNPIIPLRDLRIRKVKVIRTPKFDAQALIAAHGEVPTSAEGEARVVEEAQEETA q57yl6_tryb2 40S ribosomal protein S8 Tb927.8.6150 Tb927.8.6160 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MGIVRSRLHKRKITGGKTKIHRKRMKAELGRLPANTKLGPRRVSPVRARGGNFKLRGLRLDTGNFAWGTEASAQRARILDVVYNATSNELVRTKTLVKNCIVVVDAAPFRLWYAKHYGIDLGASKSKKATQKSTEKKSKKSAHAAVEKYDVKKASSKLKRKWEYRRKHHKIEKALADQLREGRLLARITSRPGQTGRADGALLEGAELQFYLKKLDKKKR q38fd9_tryb2 40S ribosomal protein S7 Tb09.160.2550 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSAQPHLRKLRKLKRANPSQEEESVARVLFELEGSHKTLRAQLPRFHINTVRTSSSPRHKKTAMIILYPLRFIMLVRKIQRTLTAELEKRFPGNIVVLVAQRKITKRPNDVYKLQQVQRSRTSVAVFENILNDLIYPCDVVGRRWRYRTDGSKLMKVFLDARDRKRVESRLPLLAHVYKLLTHRTVTFGFMWNPKLQQVSSR q38cl9_tryb2 40S ribosomal protein S6 Tb10.100.0080 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MKLNVAYPRNGTVKQVEVTDEVLRRVNLGDYRLGNEVDGAIFGEAFRGYTFKLRGGSDKEGFPMVQGVMAPSRVSLLVKRGAVGFNTFRGYQGERRRKSLRGCILGSDIAVLNVTVEKVGEQPIEGVTDVSVPRRLGPKRANKIRKLFNLGRTDDVRKYVIRRKVTKEGKKDRFKAPKIQRLITSTIRARRAKKVRVAIDKVRKSAAERREYLRLVGARRRAARQRKAARHHSSRVNAQRKEVDAFKARK q385d9_tryb2 Tb11.02.4170 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSAKAPKLFNKWSYENLQTTEIALNDYITRTPTYVPHSAGRWQKKRFRKARIPIVERLTNGLMFKGRGNGKKLQAVRLVRHTLEIIHLLTDQNPIQVVIDAVSKGAPREDSTRVGAGGVVRRQAVDVSPMRRVNEAIYLMCKGAREAAFRNLKTLPECLADEIVNASKGSSNSYAIKKKDEVERVAKANR q383i8_tryb2 40S ribosomal protein SA Tb11.01.2680 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTETSRRDLIFLVLSPFSPSLSSKALRNSHTRNMTSVSSGAKVLRMKESDVQKLLAMHCHLGTKNSSSAMKKYIHGRTSDGTNIIDIHMTWEKLILAARVIAAVENPQDVTVCSTRLFGQRAIFKFSQLVGTSFLGGRFIPGTFTNQIQKKFMQPRVLVVTDPRTDHQALREASLVNIPVIAFCDTDAPLEFVDIAIPCNNRGRHSISMMYWLLAREVLRLRGTIPRSVPWEVKVDLFFYRDPEEALKQEEATAAAEVHEEADEGYGWVERDNTWEQ q57wi3_tryb2 60S ribosomal protein L40 Ubiquitin-60S ribosomal protein L40 Tb927.7.3680 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MQLFLRSATGATSVVEASAADTVGTLRAKAGFDDTSSIFFFGGFCLREESATLAECGLQQGSTVQVMIPVEGGKGKKKKKKVFTKPKKPIHRHKLEKMRALKYFKVTENDDGSFKVERTRDECPNPNCGAGVFMAQHKGRKYCGKCHLTYTMK q383e1_tryb2 Guanine nucleotide-binding protein subunit beta-like protein Tb11.01.3170 Tb11.01.3180 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MAVAYEGQLTGHRGWVTSLACPQTPETATKVVSTSRDKTLLSWGPNPDRHSSECSYGLPDRRLEGHSAFVSDVALSNNGNFAVSASWDHSLRLWNLQNGQCQYKFLGHTKDVLSVAFSPDNRQIVSGGRDNALRVWNVKGECMHTLSRGAHTDWVSCVRFSPSLDAPVIVSGGWDNLVKVWDLATGRLVTDLKGHTNYVTSVTVSPDGSLCASSDKDGVARLWDLTKGEALSEMAAGAPINQICFSPNRYWMCAATEKGIRIFDLENKDIIVELAPEHQGSKKIVPECVSIAWSADGSTLYSGYTDNVIRVWGVSENA q57vp3_tryb2 Tb10.70.1380 Tb927.8.1110 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MRNYNNFNRVWKAPRRPFEKERLDREMKLCGQYGLRCKREIWRVNMTLSKMRRTARLLLTLPENHPRRLLEGSAIMRRCHGYGFLDEDKDKLDYVLSLTVPDILERRLQTVVFKHGLAKSVHHSRVLIQQRHIAVAKQIVTIPSFIVRVSSEHHIAFADASPFGNGRPGRVKRVKRNAAKKGSGGGDDDE q586c2_tryb2 Tb10.61.1390 Tb927.2.5910 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVRMHGNGHGKAASALPYRRTPPTWLKSSSRDVIDAVCKLAKKGLSPSRIGMQLRDSMGIAQVKNVTGRKILRILKHKGMAPEIPEDLYCLIKRATQMRKHLERNTKDKDTKFRLILVESRIHRLARYYRRVKQLPPTWKYESSTASAMVA q385y8_tryb2 40S ribosomal protein S12 Tb11.02.1830 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MAEETSLVADKVPEPAVIDAVADAMPDSLEDALRIVLMKARETNGLICGLSEVTRALDRRTAHLCVLADDCEDEEYKKLVTALAKQNNIDLVSMDEREKLAQWAGLTRMAADGSVRKTLKCSCLAVRDFGERTKALDYLLSQLQ q38ci4_tryb2 Tb10.70.7695 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTSKQENYARTPTVDLTVQHEKAYQRQTAVNENIRTASKKHVNRSGHIRYSKKIGLGFATPKAAINGKYIDRKCPFTSNVSIRGRILRGVVHSTKMRRSIVIRRNYLHFIRKYQRYQKRHRNITVHCSPCFDPKPGDEVVVGQCRPLSKTIRYNVLQVVSKSAADKMGKKFSKN q38b66_tryb2 Tb10.70.1670 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTTYVPKSSRDDVYRFFFTEGVVSCKKDPLGTWKGTLGGKTFKLPTLQVMQLMRSLKSRGLIKEQFAWRHFYWTLNDEGINYMRKYLYLGADAVPNTHKVDHKTFEREGGRGRGRGDGRGRGRGFGRGRGEGRGFGRGRGEGRGRGFHSERDQYRAGAAAQGEDAAPVAAVE q57vv0_tryb2 Tb927.7.1040 Tb927.7.1050 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSGEKTHTLKQVQTFGKKKTAIAVATVTKAPQCNIRINGVPISQILPETLRAKIMEAVKVVGARYFSRLRVDVRVRGSGQVAQAYAVRQAIAKGIIAYYQKYHNEIEKAALKDKYLEYDKFLLIADPRRCEPKKWGRHSARTRFTKSYR q580k6_tryb2 Tb11.02.4000 Tb927.3.4360 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTMMSVLANALRCIAGAERRGKRQVLIRPSSKVVVKFLQVMQKHGYIGEFEIVDDHRAGKIVVNLNGRLNKCGAICPRFDCTARDFEKWVKNILPSRQFGFVVLTTSLGIMDHEEARARNTGGKVLGFFY q57xn7_tryb2 Tb927.7.2340 Tb927.7.2370 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MASNITQERYEQLKKERTFHKFTYRGHEIDPLLALTEEEFKALVHARARRRMNRHADRRAPVLLKRLREAKKNVKAGEKPKAVKTHLRDVVITPEMVGSVVGIYNGRQFNAVEIKGEMIGHYLGEFSLTYKPVAHGRPGFGATHSSRFIPLK q4fkg9_tryb2 Tb11.0290 Tb11.0390 Tb927.6.4980 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSKKQEVKYYGSSAGKDQLVYGVVHIYASFNDTFVHVTDMSGRETFCKVTGGMKVKADRDESSPYAAMMAAQDVVARCKECGINALHVKMRATGGVGTKSPGPGAQAALRALARAGMKIGRIEDVTPVPTDSTRRKGSRRGRRL q388d3_tryb2 Tb10.61.1960 Tb10.61.2070 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MADAPRGTGGEQRGFGRGRGGRGGRGGRGGRGGRGGGGDEKEWVPCTKLGRLVKDQKITSLEEIFLFSMPIKEHQIVDQLIREGDLRDEMMLVFPVQKATSAGQRTRFKAFNVVGDGNGHIGVGARVGKEVSLAIRASMIAAKLSIVPVRRGYWGNKIGEPHTIPIKVTGKCGSVAVRLIPAPRGAGIVAAPVPKKILEFAGVEDVYTSSCGKTRTRGNFIMATFYALRKTYGFLTPDLWAETEVSRDPTDEHSEFLSMGRKMVAA q583k7_tryb2 Tb927.4.1860 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MAVARTKANSKIRRIGKKKGATLRDVHPWRWVKICSQHFKQEGKMMVPNCAEIVKTSHGRERAPQNPDWYYIRCAAVLRAVYLRPGVGYGGLSKRFSSKKNRGSRPEITTRASKGLLHWCCKSLTKLELLEKCKGAGHRVTKLGRKVADTIAFKIALRKMGAETKAK q38b69_tryb2 Tb10.70.1730 Tb10.70.1740 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSLTLQSESFQHIVRLLNTNVEGKRKVPFALRMVKGIGIRFAYMVCKKAGVDVERRAGTLSPEELEKISEVIADPAKFKIPEWFLNRQRDPKTGKTEHLTSSMVDTRLREDLERLRKIRAHRGVRHAYGLRVRGQHTCTSGRRGKTVGVSRTK q382z8_tryb2 Tb11.01.3675 Tb11.01.3676 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MGKIRTKTVKRASKQIVEKYFSKLNKDFYQNKRIVMDVTIARSKKLKNKIAGYATHIMKRLARGPVRGISLKLQEEERERRMDYVPEVSHVDQAIQDGIRVDKQTLAMLKRMETGVPRHVLPNVVAAPNVSKGARRGGPARK q57xx0_tryb2 40S ribosomal protein S25 Tb10.70.4800 Tb927.3.1370 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MPPKKGGKDTKQAPKKGKMENLNKGAKKAAKKWSKGRTREALQNAVMFDKETMDKLMKEVPKYKVITPSIISDRLKISVALAGKGLQHLCRQGLIRLVSCSSKFRVYTRAATA q38am9_tryb2 40S ribosomal protein S24 Tb10.6k15.3340 Tb10.6k15.3350 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVFQKKKAEVTVRTSQFKVNKLLNRKQFVVEVNHPGWCGTVPAKLIRNRLASLYKVADENQISVFGFKTKFGGGKTTGFGLIYDDLAAMKRIEPNYRKARLGMGKKKLPARKSVKERRNRNKKIRGKAKGKMQTKKK q38cd1_tryb2 Tb10.70.7020 Tb10.70.7030 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTKTNGQHAARKLVRLRRKNRWADKGWKKSHTFVAQKANPFGGSSHAKGIVLEKIGVGAKQPNSAIRKCVRVQLIKNDKKIIAFVPNDGCLHFIEENDEVLVSGFGRSGHAVGDIPGVRFKIVKVSSVGLYALYRQKKEKPRN q385b8_tryb2 Tb11.02.4360 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MATIGTYNEEGVNVDLYIPRKCHATNSLITSFDHSAVQIAIANVDPNGVIDGTTTTLCIAGYLRCQGESDHAINHLAIAKGIVRIKTGKKPRAKKTKNLKVASGARTQQKGGRNAAGQTKGAAQRGAAQKPTGRPQQAQRQGGRPARTEEGNRQRQGQQRQKPQQQQQQRQQQQQQRGGDRRAPQQSGRRGPRS q584u5_tryb2 40S ribosomal protein S30 Tb927.6.2110 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MGKVHGSLARAGKVKNQTPKVSKQEKKKQPRGRAFKRLKYTQRYLAKTLKAGEKLRMNKQPPGKAG q389i7_tryb2 Tb10.26.0370 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MGPLSKKRMMVRDGVFYAELFEFLKRELADDGFAGVEHRVTPTRTEIVIRSTKTREVLGEKGRRIRELTACLQQRFNYKEGKLQLFAERVEVRGLSAMAQAESLRFKLLSNLQVRRAAMGIIRYVMESGAKGCEVTIGGKIKGQRAKSMTFRDGYMIKSGTAHKHFVDTATRHCHLRAGTIGVNVKIMRPQSMIEEDEVLPDVITVIEPKTIEA q383v7_tryb2 Tb11.01.1475 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MGFFDSDLSYPTVRTERMKHKRRRLVQGPNSYFMDVKCPGCKNITVVYSHATSEVKCNGCATLLCRPTGGKAMLVTGCGFRKKPDH q384j9_tryb2 40S ribosomal protein S26 Tb11.01.0355 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTTKRRNHGRAKPPCSRGRVRPVHCFNCSRLVPKDKSVGRFVVRRMLDQASARDVAEASLIYGSGFPMPKLYMKQRYCIACAIHSRTVRARPVEDRKRRYTSKVPFRPTGK q38ec5_tryb2 Tb09.211.0110 Tb09.211.0340 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MARRPARCYRFCKNKPYPKSRFCRGVPDPRIRTFDIGKRRAPVDEFPVCVHVVSRELEQISSEALEAARIQANKYMVKRANKECFHMRIRAHPFHVLRINKMLSCAGADRLQTGMRQSYGKPNGTCARVRIGQILLSMRTKDTYVPQALESLRRAKMKFPGRQIIVISKYWGFTNILRNEYEELRDAGKLQQRGLHVKLITPKGKITPYNIMA q383v8_tryb2 Ribosomal protein Tb10.05.0220 Tb11.01.1470 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSKIPPALLSEAIQNVLKDRKERKFKESIDLQVNLKNYDPQKDKRFSGSVRLPHVCRPRMTVCLLCDLVHEDIAKKNDVPTMNQEELKKLNKNKKLVKKMCNQYDAFLCSESIIKTVPRLVGPHMHRVGKFPTVCAQNESLPDKVLELQSTVKFQLKKVLCLGTCVGHVDMTEDQVRQNVVMAINFLVSLLKKNWQNLKSAYIKSTMGKSQRIY rl18_tryb2 60S ribosomal protein L18 RPL18-A Tb10.6k15.0410 RPL18-B Tb11.02.1840 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MGVDLTGVQKKKRVVRHHTYSTNPYIKLLIKLYKFLGKRTNSPFNKLIHKRLLKSRNNRAPISLSRIAVCMRRRTVWLKKGKKSPIAVIVGDVLDDVRMTRIPALRICALRFSKSARERITGAGGECLTFDQLAMMAPTGKNTMLLRGRKSGRESVKHFGAAGVPGSHAKPHVSSRGKERQRSSKRRHAFRHK q57u30_tryb2 Tb927.7.230 Tb927.7.240 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MCSSVAMAEVATAATTAATKKDKKAPQEEVVVQQTDQAQVGVIIKVLGRTGSRGNVTQVRVRLMAEEGSPEANRTIVRNVKGPCKEGDMLSLMETEREARRLR q585j5_tryb2 Tb927.4.3550 Tb927.5.1610 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVLPSRRSLNRSSRKGFKKHRPDIIVIDLKDHVLGRAAAIVAKQLLLGKKITVVRCEKLTIAGSEIRNKIKYLQFLRKRKLSNPKLGPFHHRSPSDVFIRTVRSMLPRYTKRGQRALRQLVAYEGVPVNVVRTGGRVVIPKAQRHNCYRNERRFTVLGNMCKHVGWKYSDVVEKLEAARIEKSGRHHKKMEKVRVAWKNARKEALKKMPQKNVEVLKKFGLA q57v54_tryb2 60S ribosomal protein L13 Tb927.3.3320 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MPKGNNAIPHVHQRKHWNPCSSQKGNVKVFFNQPAQKQRRRRLRLLKAKKIFPRPLKALRPQVNCPTVRYNMKRRLGRGFSLEELKAAGVKPRYARTIGIRVDRRRKNKSEEGMNINVQRLKTYMSKLVLFPLNRKKPQKGDATEEEVKAATQDRSRYGTAAVGGLVTPAREAPRKVTEEESTKKMYKFLKKNHSAVRFFRARNRRAARKEAKENEKK q57yk4_tryb2 Tb927.8.6030 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MPPKFDPNQEITVVVRAVGGEVPATASLAPKVGPLGLNAKKIGEDIAKSTKDWKGLKVTCQLRVKNRVATVVVTPSVASRLIRALKEPPRDRKKVKNIKHDGNIAFSEILKIAKESAPNSMGASMKSVVMEVLGTAVSIGCTIDGEHPRAIQEKVQEGKLKVPN q38ei9_tryb2 Tb09.160.5580 Tb09.160.5590 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MAEAKVSAEKKAANPMREIVVKKLCINICVGESGDRLTRASKVLEQLCEQTPVLSRARLTVRTFGIRRNEKIAVHCTVRGKKAEELLEKGLKVKEFELKSYNFSDTGSFGFGISEHIDLGIKYDPSTGIYGMDFYVVLGRRGERVAHRKRRVSRVGFNHRVRKEEAMKWFEKVHDGIIFQAKKKKKSMVRRRRR q382c4_tryb2 60S ribosomal protein L18a Tb10.70.3510 Tb11.01.5720 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVRPHLRHYCVVGRETPSEKNPQPTVYKFEVFAPNFVVAKSRFWRMMREKNKVKSTHGDVLSCKVVKDRKLAARNYSVDIAYYSQRCGYTHMVKEFRDVCKAGAVSQAYNDLASRHRARYHNIEVLGVKSIPNHEVRRLNVAQFHPHNLSFPLLQRRVKAPRKDRVIFVKKNSKRAVVA q385t6_tryb2 Tb10.61.2090 Tb11.02.2430 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVHYSRKPQVSSKTAKAKIADLRCHYKNTFETANVINGMKLRKAQQLYRQVLAKTRCIPFKRYNGKIGNTAQAKEWGQTKGRWPRKSVVAMLSLLKNAEANAIEKGLDPGKMVIKHVQVDQAPRVRRRTFRAHGRITPYMRSPCHVQLFMTQPQERVPVPKSKPKK q38cy7_tryb2 Ribosomal protein L15 Tb09.244.2720 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSHSVYYYFTFESSPAPMGSFMYLNELWKKKSSDVMRFIQRVRAWEFRHQHTIVRLRRPTRPEKARMVGYKTKQGYVVFRVRVRRGGRKRPVHKGITYGKPNTAGVLGRKLNKNNRVVAEQRLGKKYGNLRVLNSYWVNADSTFLWYEVVAVDPMHRAIRRDPRINWIVNAVHKHREQRGLTSAGRKHRGLRQKGHKASKLRPSRRAAWRRNNRMVFLRKR rl14_trybb Probable 60S ribosomal protein L14 23 kDa cell surface protein homolog protein protein trybb Trypanosoma brucei brucei MVKANYIRAGRLVRILRGPRQNRVGVIVDIVDANRVLVENPCEQKMWRHVQNLKNVEPLKFCVSISRNCSTKALKEALESKKVLEKYAATKVRRPHRAKKAFAESTDFERYQLRVAKRSRAYWARKIFDENDKKNPVSWHKVALKKLLKNAKKVDSTPAAKKRVEKARAARKARLAAGKSKTKVAASKK q38dp2_tryb2 Tb09.211.2630 Tb09.211.2640 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MGKDKANVKGCRFRVSLALPVGAVVNCADNTGAKNLYIISVKGYHGRLNRLPAAALGDMVMASVKKGKPELRRKVLNAVIIRQRKSWRRKDGTVIYFEDNAGVIVNPKGEMKGSGIAGPVAKEAAELWPKISTHAPAIV q383r3_tryb2 Tb11.01.1920 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVAVRAKVGSRGFTRQRQLAKGKKVFKIDCSIPASDGIFSDDILSNFQQYFQDNVKLNGRKGKLTSKVRVNMRENTLSITTTMAYRKKYFKYLTKKFLKKKDLRDWIRILAKGKDTYQLKYFNIQDQEEA q4fkd4_tryb2 Tb11.0880 Tb11.50.0005 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVHSHGYKCGTRHLFAKKFRKHGVPSVSTILTNFKVGDYVDVVADAAVRAGMPHKYYHGRTGIVWNVTPRGVGVIINKPVRNRTVRKRICVRFEHVRKSRCQEAFKQKLKDLEAFRAAKKAGTPLPPKKVSTRNGGFVCPKKVEVLARRTADYEAMIPY q57zi7_tryb2 Tb927.7.5000 Tb927.7.5020 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVSLKLQARLAADILRCGRGRVWLDPNEAVEIRNANSRKSVRKLIKDGLVMRKPVKVHSRSRWRQMKLAKSMGRHEGTGRREGTREARMPSKDLWMRRLRILRRLLRKYREEKKIDRHIYRELYMKAKGNVFRNKRNLMEHIHKVKNEKKKARQLAEQLAAKRLKDEQNRRKARKQELKKREKERERARRDDAAAAAAAKQRAAAKKAAAPAAKKGGKAVAPATPAKAAPAKAAAAKVAPAKAAPAKAASPAGKKAAGKK d7sg81_tryb2-q381j6_tryb2-1 3943267 N/A 3943268 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943269 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943270 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943271 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943272 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943273 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943274 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943275 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943276 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943277 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943278 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943279 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943280 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943281 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943282 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943283 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943284 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943285 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943286 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943287 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943288 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943289 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943290 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943291 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943292 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943293 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943294 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943295 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943296 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943297 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943298 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943299 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943300 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943301 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943302 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943303 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943304 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943305 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943306 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943307 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943308 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943309 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943310 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943311 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943312 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943313 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943314 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943315 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943316 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943317 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943318 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943319 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943320 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943321 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943322 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943323 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943324 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943325 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943326 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943327 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943328 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943329 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943330 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943331 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943332 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943333 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943334 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943335 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943336 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943337 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943338 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943339 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943340 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 N/A 3943341 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified in vitro In vitro Stoichiometry: 1.0 physical association physical association false false false Fig 1, Supplementary Fig 2 MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint hong-2006-1 TbGPI16 is an essential component of GPI transamidase in Trypanosoma brucei. tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Hong Y., Nagamune K., Ohishi K., Morita YS., Ashida H., Maeda Y., Kinoshita T. FEBS Lett. (0014-5793) 2006 watanabe-2016-1 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Watanabe Y., Kawaguchi K., Okuyama N., Sugawara Y., Obita T., Mizuguchi M., Morita M., Imanaka T. imanaka@pha.u-toyama.ac.jp FEBS letters 2016 Only protein-protein interactions imex curation imex curation watanabe-2016-2 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Watanabe Y., Kawaguchi K., Okuyama N., Sugawara Y., Obita T., Mizuguchi M., Morita M., Imanaka T. imanaka@pha.u-toyama.ac.jp FEBS letters 2016 Only protein-protein interactions imex curation imex curation pe_ate-2009-2 RNA pol II subunit RPB7 is required for RNA pol I-mediated transcription in Trypanosoma brucei. tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Peñate X., López-Farfán D., Landeira D., Wentland A., Vidal I., Navarro M. EMBO Rep. (1469-221X) 2009 2009-03-19 Only protein-protein interactions imex curation pe_ate-2009-1 RNA pol II subunit RPB7 is required for RNA pol I-mediated transcription in Trypanosoma brucei. tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Peñate X., López-Farfán D., Landeira D., Wentland A., Vidal I., Navarro M. EMBO Rep. (1469-221X) 2009 2009-03-19 Only protein-protein interactions imex curation pe_ate-2009-3 RNA pol II subunit RPB7 is required for RNA pol I-mediated transcription in Trypanosoma brucei. tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Peñate X., López-Farfán D., Landeira D., Wentland A., Vidal I., Navarro M. EMBO Rep. (1469-221X) 2009 2009-03-19 Only protein-protein interactions imex curation q388l3_tryb2 Tb10.61.3060 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MLPMLLWLVANLFLAPAAEGFHGMNKTNTWAVILSSSRYFFNLRHTTNALAMYHLCRKHGMDDDHILVFLSDSYACDPRKPNPATIYGAPAQAEQPNLYGCNIRVDYASYDVGVRRFLGVLQGRYDENTPPSRRLDTDENSNIIIYAAGHSAEKFFKFQDSEFMSSTDIADTLMMMWEQRRYRKLVFLVDTCRALSLCLEIKAPNVVCLASSEAHLDSYSHHLDPPSGFTVITRWTFEFLEVLKDSKCRPENGEVTLLQKSFYDFNYGPERLSLPQPLSEPAHFDAVNRPNAVREWKMDEFFCEQDRDKIPVELRYDLF mint q7ytw6_trybb TbGPI16 protein protein trybb Trypanosoma brucei brucei MVVVAALLFLLCVTTRTQALKPTVPRTERRYTVHVQPLTTGLDDDGLESTLVSLTMTTSLWLPKGSAEDREEHLSFNEELDPFWVHVLRRRRFDLIEWSGCGGSWRPEWTSTLHNRKHPVDKSTAELIHDLSHHSVCSQSTLSACGWVSTSSNGNNDGNATSAGEEDLRHHHQWLAQFVSSLLSSPLTWWDDTPRFVRPIGALHSVPAGNSVFASSGYSGKFGDVRWCSYHHAQQDTLCTQHVTALLNGGRSGKDVEERIPRGIFRAGLVSMEDFFSSVFHHFRFEVKQSPATEGGEARGNHTNEEAEIKLVMRMAFVEQNKKFDALSSHWKESFPEYAPSVDFITGSQLNAKLSSALQTLKKPQGARSGNDNQALGNVEVTPTAQHFISAVGYDRGTYRLVVNPTPEVPTSGNSNTKSRHEQSLMLQPGDTLEALLFFPLHLMRPSLHEMRAGAGLESRVENAFVDESANVLVVLVHTTVTETAMRPLEAGVQSNGFVLCEFPFRFGWSANKDMPPDANSNRILPQPVIRVGRHVSSAGGKVGEGDKASGAQNCGMSRCASISNKRIDHQGSLESLMESMRRDNNRKGPCVCYFWLRCSNVAGSTLPVPDPAMVFNVVTLGLIFSAIVAGGVVRSTKRLFDVEESGADDENPLIIRIILRIMKRLRKRRAAAAGTG mint q57w55_tryb2 Tb927.5.1100 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MDCGAGFALGQQLAKDALHMQGGVRPGTTGNVEQDALMTGMMVPPTGPMEDWAQHFAAHQHHHQQHQQMMMQRQHNDALMIQQQHRDMEEAFRASARAGAPQQANAGPLMMPPGPMMMAGGMAPMMHAGGFMMGGMPQMMPCTPMGMNMGMAPVATMNPATTSTVSGAREGATAVSSAAPGVVDLGGDSAWAEKLHQAEWGQDYKDVEVHTVEGSTAQTVEEHAKTSKFYEFMDKIRKKELLVDEDSGEVVQGPGPDPDVEADTEYLARLAAMEGINVPPSVMDHMQGQDGVQRGTDEDMEGMMGDDVYDPSADVEQWAQEYAQMQAMQERLQNNTDYPFEANNPYMYHENPMEEGLSMLKLANLAEAALAFEAVCQKEPEREEAWRSLGLTQAENEKDGLAIIALNHARMLDPKDIAVHAALAVSHTNEHNANAALASLRAWLLSQPQYEQLGSVNLQADVDIDDLNVQSEDFFFAAPNEYRECRTLLHAALEMNPNDAQLHASLGVLYNLSNNYDSAAANLRRAVELRPDDAQLWNKLGATLANGNRPQEALDAYNRALDINPGYVRVMYNMAVSYSNMSQYDLAAKQLVRAIYMQVGGTTPTGEASREATRSMWDFFRMLLNVMNRPDLVELTYAQNVEPFAKEFGLQSMLL q8iew2_trybb Peroxin-14 PEX14 protein protein trybb Trypanosoma brucei brucei MSLLLSGVVDDGKSKPEVEHTHSEREKRVSNAVEFLLDSRVRRTPTSSKVHFLKSKGLSAEEICEAFTKVGQPKTLNEIKRILSERPYVPTGPNSQHMTQPLRDESADSVPTPHPNQSRRHTSLLYAPQAPPLPEAAAATRGVDWRDLVIGAGAAVIGGFAAFKAFQLYSPYEIRLKDEGSKPRSRRSRRGGRHASSDSEAERSLVHREVPALPVPAIPVASESHVDAKQAEIERLKTELKETQEALEAEKKGKAELSITLGKLRGQVTAYSRTNEKQESQIKSLQEEVNRLKSEIERKEDSAKVNANSNVEETLDSKEEGLPSSDTESSNPNGASSEVARDTQQQDGPAPLSTETDGTVVGGEAA q381y8_tryb2 Tb11.01.6090 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MFYKMKLERNIKVEPHLLERTLHRHLEEYLRKAVEGTPLHLSLGNALRSGLARTNQNSSAVIIAVLDILNTSSLEGRVLDDGSVSFFVKYEGLVFKLHRGEVIDVIVSSVEREGWWGDVMGVGRMYISRGQMGNEWVYESDGMRGIWITKDGARSVKEGEIVRVRVVAETPQSEGVLAVGSMVGNYLGPL mint rpa1_trybb DNA-directed RNA polymerase I subunit RPA1 TRP11 DNA-directed RNA polymerase I largest subunit protein protein trybb Trypanosoma brucei brucei MSGIAFVEVRTRAGQEDRSAPWRPVVRNGENHATFYDTRMGNFDANPFPPQTCQTCAASLTGKYGNERCHGHFGFVGMPRIRPGSAHSDSDRLVVLNPHLAMDADRLFRAKCFFCHKFRAPTFDVERFRQALVLADHGLPGDALHLLDTVPTAKGHDAMLNHRRMANEEIVNDVSILQSYVDRILRQRASGCSEEDAKARVTMAQKGTVDVRNDICNMAISHLRSFSGPCSHCTAISPTFLKRGGIIFFLFRKSNLVTNIAKGFLTQQEVSEWEAVNRLHGRTGTYFDGRQMLFHMKNLFAKEQAILGLLYPNLGEPSVFTKTNKVVPASERYKLFFLDRILVPPLPLRLSSGVRVNDNGLIIPDEQTRALSDILGFVEQIECFHTLSANSTNGRSFITDAQRAVNESNLRNLQQKVDEFYAEIVNSFAKKEGLFRMNMMGKRVNQACRSVISPDPFVEPNEVLLPRPLARALSFPEQVTCFAPARMNLLKHCVVNGPRKYPGATHIELRHANGEIRSVDLNVPEQTRRQHAARFFAMAQSGVTLIVYRHILNGDRVIFNRQPTLHKPSMMGYRVKVLSGSKTIRFHYVNGNSFNADFDGDEMNVHVPQSIETRAEVETLMDANINYLVPTSGRPIRGLIQDHVAAGVLVTLRDKFFDHSTFVQLVYNGVGPYIQENVGITLAELIPIPAILMPRPMWTGKQLISVMVRFSSGLSAASDCGREIEGGITLKGTSQIQPSAFDRIPAGSCDAVRAKSGAVVDSTVMFANSELITGFMCKKQLGASNMSAPHHVYELYGPHRTGQLFAAFGRVLLLALRKEGLSLAMDDMFLVDEERRCDLLRKLDDIALDVPDEEATAAPMIADYATKIQQEFVPQRMLVPFPKNHLLLMTISGAKGSNLNATQMSLQLGQQLFDGLRVKRMNSSKTLPSFFTNEKRARSFGFAMGSFASGIRPAEYTIHAMAGRDGLIDTAVKTSRSGHLQRCLIKGLESLVVHWDRTVRDSNGSVIQFMYGGDGLDPCKASTLTAWEMMKDNVVDVSKRFGGDASESVAGAEDGAAAGLKEMRNEDGKPTTEAVQNAHMEQQLSTYPLPASLDKSLSEYLCKKADFPLFRKVSTLARWDAKQQLKERLQQRRQKWVGAFEKTLADITARRRLWALCEPGEPVGLLAAQAAGEPSTQMTLNTFHTAGSTVSHVTEGIPRLRELLIYASVNKAAVVVPVTNATEEDEKVIAKMLRAGVAAKLTDCLAKVTDGAGGQSASSSMQRNLNTGFGKGYHYHVARGRTGMVITVSFLFSRSCLEELRKRMCMSPSEHRQSFTEALKNVVRLIMRSLSAVPREKESGDGSGNTGGMKGGSGRADRKRKRSGPDDGGGPLGGTFGDEIMRIEEGTDSDDGMSERSSIGGGRAGSEVSSLHSDGTDTRGIAGSDTGGPQRRRGSVESGRGDDASDSEAADPDLYARRSGSPARDAEDGGEMQDRDGTDWGGTSMQGVVGYDNFPEIHMSFTKSNFGAVIAPLSTAAAARDGVVQLHEDFFIVNAVLRTASDVIAVIPDVVDNALEAQRMPSWLPQFGSLTFTRLKDKGSGQLVFQGPGSTMRNVMSFLSLFTVGIKSIKLHQACSTDIRDMGTYFGIESGYAALYDELNKLFNRYNVDPRHLSLIADTSTHRGRWENFNFTGVISTSASPLFQMTFASSKRWLHRAVSRGMSDDLESFSSAIMVGERPRVGTASVRLSTDTAILRDVLERNFA mint q387n1_tryb2 Tb11.03.0935 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MIQSDIVSYVGEMGDVPDALLITPLKDRKSSVYLTKYEVARIIGERARQIVNGTSVLLDSQNGCGTGNSLELAEGCADPFAASVDPIHITKLELLQGRIPMIVRRTWPDGRTENIPVNELKVDKMLLDVQH mint tbgpi16-q388l3 314633 q388l3_9tryp 314634 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level Stoichiometry: 1.0 q7ytw6_trybb 314635 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tagged flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false undefined mutation mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 physical association physical association false false false F3C F3D mint pex14-1 1922983 N/A 1922985 unspecified role unspecified role prey prey purified purified Q57W55:p.[Trp200Ala;Tyr204Ala;Trp318Ala;Tyr322Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q57W55:p.[Trp318Ala;Tyr322Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false undefined mutation mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q57W55:p.[Trp52Ala;Phe56Ala;Trp200Ala;Tyr204Ala;Trp318Ala;Tyr322Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 1922986 unspecified role unspecified role bait bait purified purified gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false f3 q57w55_tryb2-pex14-2 1922983 N/A 1922985 unspecified role unspecified role prey prey purified purified Q57W55:p.Glu321Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q57W55:p.Glu321His mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1922986 unspecified role unspecified role bait bait purified purified gst tag gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false f5 q57w55_tryb2-pex14-1 1922984 N/A 1922985 unspecified role unspecified role prey prey purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1922986 unspecified role unspecified role bait bait purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1922984 f4 pex14-q57w55_tryb2-1 1922984 N/A 1922985 unspecified role unspecified role prey prey purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1922986 unspecified role unspecified role bait bait purified purified his tag his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false 1922984 f4 q381y8-q387n1 801960 q387n1_9tryp TbRPB6z 801963 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level tap tagged tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false q381y8_9tryp TbRPB7 801964 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false F3B mint trp11-q381y8 801960 q381y8_9tryp TbRPB7 801964 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level rpa1_trybb rpa1 801965 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false F3C mint q381y8-trp11 801960 rpa1_trybb rpa1 801965 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level q381y8_9tryp TbRPB7 801964 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false F3C mint q387n1-q381y8 801961 q381y8_9tryp TbRPB7 801964 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level rpa1_trybb rpa1 801965 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level q387n1_9tryp TbRPB6z 801963 predetermined predetermined participant predetermined unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level tap tagged tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false F3A mint q381y8-trp11-1 801962 rpa1_trybb rpa1 801965 immunostaining immunostaining unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell q381y8_9tryp TbRPB7 801964 immunostaining immunostaining unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell colocalization colocalization false false false F4 mint