MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint wiegand-1995-1 The crystal structure of porcine pancreatic alpha-amylase in complex with the microbial inhibitor Tendamistat. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wiegand G., Epp O., Huber R. J. Mol. Biol. (0022-2836) 1995 binter-2011-1 Characterization of the PLP-dependent aminotransferase NikK from Streptomyces tendae and its putative role in nikkomycin biosynthesis. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Binter A., Oberdorfer G., Hofzumahaus S., Nerstheimer S., Altenbacher G., Gruber K., Macheroux P. FEBS J. (1742-464X) 2011 Only protein-protein interactions imex curation q712i5_strte nikK protein protein strte Streptomyces tendae MPAREGADSPFLRESAGRFRNRRVRVPDEINLKNCGLLDSRAAAVHRATLAEFDPNDVLTYPILQPVYGMLADRFGVDTDSLVLTAGSDPGLNLLTRAFPEVSRIVLHQPNFDGWAKFAAISGCVLDPVAPDPETGLFDLRDLARRLRAGAPAFVVVTTPHSFTGQVHGREELAELADAVAEHGSLLVVDTAYLAFTEGGEELVRGLAGLRHVVRVNTFSKSYGLSGARIAVTVAHPATARHLFDLDPEGSVSAPAVALLRRSLEEQAVFTAIWADVRRLRERFAAEVERAVPGWHARPSGGNFVTWDVPGPADAGAASRHLLGRGIVVRDLSGAPGLPAAVRIAVAKHAVVRQVVAALGDRYREGVA mint iaa_strte Alpha-amylase inhibitor HOE-467A Tendamistat protein protein strte Streptomyces tendae MRVRALRLAALVGAGAALALSPLAAGPASADTTVSEPAPSCVTLYQSWRYSQADNGCAQTVTVKVVYEDDTEGLCYAVAPGQITTVGDGYIGSHGHARYLARCL mint Inhibits mammalian alpha-amylases specifically but has no action on plant and microbial alpha-amylases. Forms a tight stoichiometric 1:1 complex with alpha-amylase amyp_pig Pancreatic alpha-amylase AMY2 1,4-alpha-D-glucan glucanohydrolase protein protein pig Sus scrofa (Pig) Pig MKLFLLLSAFGFCWAQYAPQTQSGRTSIVHLFEWRWVDIALECERYLGPKGFGGVQVSPPNENIVVTNPSRPWWERYQPVSYKLCTRSGNENEFRDMVTRCNNVGVRIYVDAVINHMCGSGAAAGTGTTCGSYCNPGNREFPAVPYSAWDFNDGKCKTASGGIESYNDPYQVRDCQLVGLLDLALEKDYVRSMIADYLNKLIDIGVAGFRIDASKHMWPGDIKAVLDKLHNLNTNWFPAGSRPFIFQEVIDLGGEAIQSSEYFGNGRVTEFKYGAKLGTVVRKWSGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGASILTFWDARLYKVAVGFMLAHPYGFTRVMSSYRWARNFVNGQDVNDWIGPPNNNGVIKEVTINADTTCGNDWVCEHRWRQIRNMVWFRNVVDGQPFANWWANGSNQVAFGRGNRGFIVFNNDDWQLSSTLQTGLPGGTYCDVISGDKVGNSCTGIKVYVSSDGTAQFSISNSAEDPFIAIHAESKL mint amy2-iaa 101191 amyp_pig 101192 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role unspecified role unspecified role purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 iaa_strte 101193 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role unspecified role unspecified role purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 direct interaction direct interaction false false false In the xray complex 1bvn, P00690 is chain P and P01092 is chain T Resolution 2.500 mint nikk 1180008 q712i5_strte nikK 1180009 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 2.0 direct interaction direct interaction false false false mint sf3