IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ wang-2004a-1 Molecular surface of tarantula toxins interacting with voltage sensors in K(v) channels. xenla-oocyte Xenopus laevis oocyte Oocyte biochemical biochemical predetermined predetermined participant predetermined Wang JM., Roh SH., Kim S., Lee CW., Kim JI., Swartz KJ. The inhibitory activity of the toxin was examined against a mutant delat7 channel (to enable to subtract background conductance) in Xenopus oocyte using two-electrode voltage-clamp. The toxin was added to the recording chamber. J. Gen. Physiol. (0022-1295) 2004 tx1_strgf Kappa-theraphotoxin-Scg1a SGTx1 SgTx-I protein protein strgf Stromatopelma calceatum griseipes (Feather leg baboon tarantula) Scodra griseipes TCRYLFGGCKTTADCCKHLACRSDGKYCAWDGTF kcnb1_human Potassium voltage-gated channel subfamily B member 1 Voltage-gated potassium channel subunit Kv2.1 KCNB1 Delayed rectifier potassium channel 1 protein protein human Homo sapiens Human MPAGMTKHGSRSTSSLPPEPMEIVRSKACSRRVRLNVGGLAHEVLWRTLDRLPRTRLGKLRDCNTHDSLLEVCDDYSLDDNEYFFDRHPGAFTSILNFYRTGRLHMMEEMCALSFSQELDYWGIDEIYLESCCQARYHQKKEQMNEELKREAETLREREGEEFDNTCCAEKRKKLWDLLEKPNSSVAAKILAIISIMFIVLSTIALSLNTLPELQSLDEFGQSTDNPQLAHVEAVCIAWFTMEYLLRFLSSPKKWKFFKGPLNAIDLLAILPYYVTIFLTESNKSVLQFQNVRRVVQIFRIMRILRILKLARHSTGLQSLGFTLRRSYNELGLLILFLAMGIMIFSSLVFFAEKDEDDTKFKSIPASFWWATITMTTVGYGDIYPKTLLGKIVGGLCCIAGVLVIALPIPIIVNNFSEFYKEQKRQEKAIKRREALERAKRNGSIVSMNMKDAFARSIEMMDIVVEKNGENMGKKDKVQDNHLSPNKWKWTKRTLSETSSSKSFETKEQGSPEKARSSSSPQHLNVQQLEDMYNKMAKTQSQPILNTKESAAQSKPKEELEMESIPSPVAPLPTRTEGVIDMRSMSSIDSFISCATDFPEATRFSHSPLTSLPSKTGGSTAPEVGWRGALGASGGRFVEANPSPDASQHSSFFIESPKSSMKTNNPLKLRALKVNFMEGDPSPLLPVLGMYHDPLRNRGSAAAAVAGLECATLLDKAVLSPESSIYTTASAKTPPRSPEKHTAIAFNFEAGVHQYIDADTDDEGQLLYSVDSSPPKSLPGSTSPKFSTGTRSEKNHFESSPLPTSPKFLRQNCIYSTEALTGKGPSGQEKCKLENHISPDVRVLPGGGAHGSTRDQSI tx1-kcnb1-1 Patch-clamp: interaction between tarantula SGTx toxin and KcNB1 channel 177361 N/A 177362 unspecified role unspecified role neutral component neutral component P56855:p.Arg3Ala DDG>3.0, kd >5.10-4 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P56855:p.Leu5Ala DDG>3.0, kd>5.10-4 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P56855:p.Phe6Ala DDG>3.4, kd>10-3 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P56855:p.Arg22Ala DDG>3.0, kd>5.10-4 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P56855:p.Trp30Ala DDG=3.4, kd>10-3 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P56855:p.Asp31Ala DDG=1.4, Kd=3.10-5 mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 177363 unspecified role unspecified role neutral component neutral component xenla-oocyte Xenopus laevis oocyte Oocyte physical association physical association false false false 177361 Kd for the following mutants is : kd>10-3 for W24A, kd >5.10-4 for R3A, L5A, R22A, kd= Kd=3.10-5 for D31A. Kd values were obtained by fitting a four site model.