mpidb The Microbial Protein Interaction Database yen-2011-1 Asp2 and Asp3 interact directly with GspB, the export substrate of the Streptococcus gordonii accessory Sec System. ecolx Escherichia coli anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yen Y.T., Seepersaud R., Bensing B.A., Sullam P.M. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation yen-2011-2 Asp2 and Asp3 interact directly with GspB, the export substrate of the Streptococcus gordonii accessory Sec System. in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Yen Y.T., Seepersaud R., Bensing B.A., Sullam P.M. J. Bacteriol. (0021-9193) 2011 Only protein-protein interactions imex curation asp3_strgn Accessory Sec system protein Asp3 asp3 Accessory secretory protein Asp3 Orf3 protein protein streptococcus gordon Streptococcus gordonii MKIQKHKEIYWGELRGASISKTRKDFTYLYGSTIIFHSPDQVYFENKLIASGQTIHEWSSSWNYQGDRQVPSLPLLKRGRSYSLTRDMTSYPSESVFLKLIFFDRYNREVSNHVERSDKMTFTYPEEAYSYKVQLLSAGVESFEFHCLRIEEILEESNG asp2_strgn Accessory Sec system protein Asp2 asp2 Accessory secretory protein Asp2 Orf2 protein protein streptococcus gordon Streptococcus gordonii MKNKLKILQIGSIDWSKEVVIPDNMDWYYFFSLTLRLAIKKVMEMEKINHFSAIIVDDLDLIPDLFLIESRIIPYTIFYSKKQQAIQEPIAFFLKRYCAQQIDLSDRPNLLRKLSKALFRGQYGDKMTPLDMVVSPGFKGRICHNGYENLELEGNFGSDFRPIVSWKYNIVASKKNPVEIWLEYEKDLSCELRLRIYNIQEGSAADLVRESVFSETDMEETIVLDNDFTSFLGITLEARGFGTLKIGAFHQRLTRYQFGKFVLGGKILKDSHRQEINYFFYPGDFKPPLVVYFSGYRRAEGFEGFGMMRGLGCPFLLISDQRLDGGVFYLGSDELEEGIRRIIQEHMELLGFSERELILSGISMGTYGAAYYGADFSPRAIILCKPLANLGTIAQRGRLRLPEVFPMALDILHRHTGGKDRENVMELDNRYWKKFKKADFSRTIFGLAYMKEEDYDPTAYEDLVQYLYPTETQLMSNGLSGRHNDDSTMVINWFMNYHRIILEKEFGRKK gspb_strgn Platelet binding protein GspB Adhesin GspB gspB Serine-rich adhesin for platelets Serine-rich repeat protein GspB protein protein streptococcus gordon Streptococcus gordonii MFFKRQKGQYHEVERVTRFKLIKSGKHWLRAATSQFGLLRLMKGSDVSSTEVKVVEEQSVEKSGLNYLKGIIATGAVLGGAVVTSSSVYAEEEQAHEKVIDTRDVLATRGEAVLSEEAATTLSSTEANPVESLSDTLSASESTSASSSVSTSISVSESFSVSGSLSYSTSLSQSVSASASASESLSVSSSASDSVSASTSTSASASQSVSASQKSTISTSESTRSESSQQSTEASSQTGRRRTRRAVTESAPNVEYHDVKGDMIQSVTTSFDDTSRLLTWTINLTPRQVKSNLGALVSISGNQETRTVTINGKNAANGGVYNSGGAWNLYTGESVNNNVLRITTQVNDTGGEVKLGLRLVTSDKKITKTNLPLEFSQVAATTNGSWDKAGYNTTIVEKDTERPVVNVPSEITVYRGESFEYFATVTDNSNAFDLAKTVVRWLYNNQPGRGTEWLQYSVTQVGNQLKVRIFGNVPIDTTIGDYTRYVVATDAAGNVNATQTEMGNAAVDKTSVNGQFKLIIRFRIKTPENTVFVNNPNQLTEVEKNLVREAVKKSNPDLRAQDVLNSNYVTGITVSNNGTTTITYRDGRKDIIDGSKFIDTRAGSISKSQSTSNSISVSLSKSESASASLVTSKLNSISSSASVSASTSISTSGSVSASESASTSSSVSASESASTSASVSASESASTSASVSASTSASTSASVSASTSASTSASTSASKSASTSASVSASTSASTSASVSASESASTSASVSASTSASTSASVSASTSASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSASTSASTSASVSASASASTSASVSASTSASTSASVSASASASTSASVSASTSASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASTSASTSASVSASTSASTSASVSASTSASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSASESASTSASESASESASTSASVSASESASTSASVSASESSSTSASVSASESSSTSASVSASESASTSASVSASESASTSASESASESASTSASVSASESASTSASVSASESASTSASVSASESVSTSASVSASESASTSASVSASESASTSASESASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESVSTSASVSASESASTSASVSASESASTSASESASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSTSVSTSTSASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASTSTSTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASKSASTSESASTSASVSASESASTSASVSASESASTSASVSASESVSTSASVSASDSASISASVLASESASTSASVSASESASTSASVSASESASTSASVSASESASTSSSVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSSSESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASMSASTSASVSVSESTSTSASVSANESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSANESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSANESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASESASTSASVSASTSASTSASVSASESASTSASASASESASTSASVSASESASTSASVSASESASTSASVSASESASTNASVSVSESMSVSESLSLSISTSVLHSQLNDIYESELYSLSLSESLSASQSLSQSLSESQSSSASQSMHDRISKGQLPRTGESENKASILALGLGALGLAFKKRKKNESED gspb-asp2-3 1338484 n/a 1338486 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-581159341 1338487 unspecified role unspecified role prey prey q9aet8-- Q9AET8-- ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1423502257 1338488 unspecified role unspecified role bait bait q939n5-736-736 Q939N5-736-736 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false association association false false false Figure 2 asp3-gspb 1338484 not-defined-1870377865 1338486 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1750047989 1338488 unspecified role unspecified role prey prey q939n5-736-736 Q939N5-736-736 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 3 asp2-gspb 1338484 not-defined-892638198 1338488 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false q939n5-736-736 Q939N5-736-736 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged certain certain sequence position certain certain certain sequence position certain false not-defined-1002185549 1338487 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 3 gspb-asp3-2 1338484 not-defined-672009151 1338486 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-815359027 1338488 unspecified role unspecified role bait bait q939n5-736-736 Q939N5-736-736 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 3 gspb-asp2-2 1338484 not-defined-1947530876 1338488 unspecified role unspecified role bait bait q939n5-736-736 Q939N5-736-736 flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false not-defined-932901893 1338487 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 3 asp3-gspb-1 1338485 not-defined-231778256 1338486 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-183065696 1338488 unspecified role unspecified role prey prey q939n5-- Q939N5-- gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region Q939N5-1-117 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false figure 4, 5, 6 Glycosylation of GspB prevents Asp binding asp2-gspb-1 1338485 not-defined-1050745311 1338488 unspecified role unspecified role prey prey region Q939N5-- sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region Q939N5-1-117 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false q939n5-- Q939N5-- gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-1470169753 1338487 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false figure 4, 5, 6 Glycosylation of GspB prevents Asp binding