IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ ang-1998-3 Molecular interaction between COP1 and HY5 defines a regulatory switch for light control of Arabidopsis development. in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ang LH., Chattopadhyay S., Wei N., Oyama T., Okada K., Batschauer A., Deng XW. xingwang.deng@yale.edu Mol. Cell (1097-2765) 1998 Only protein-protein interactions imex curation hardtke-2000-3 HY5 stability and activity in arabidopsis is regulated by phosphorylation in its COP1 binding domain. in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Hardtke CS., Gohda K., Osterlund MT., Oyama T., Okada K., Deng XW. It was not possible to determine the species of origin of the RBCS1a promoter , therefore interactions involving this promoter were not curated. details of the CHS1 promoter were taken from a reference provided in the text. xingwang.deng@yale.edu EMBO J. (0261-4189) 2000 Only protein-protein interactions imex curation chs-sinal-dna Chalcone synthase promoter Sinapis alba ds dna double stranded deoxyribonucleic acid ds DNA ds dna sinal Sinapis alba (White mustard) Brassica hirta White mustard The authors in the relevant paper (see PMID:9659918) describe this as "180 bp fragment of the CHS promoter that includes the Unit 1 region: 2247 to 268". They reference the promoter as the CHS promoter region from white mustard (Sinapis alba) and reference this with PMID 7599309. hy5_arath Transcription factor HY5 HY5 BZIP56 At5g11260 F2I11_150 Protein LONG HYPOCOTYL 5 bZIP transcription factor 56 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQEQATSSLAASSLPSSSERSSSSAPHLEIKEGIESDEEIRRVPEFGGEAVGKETSGRESGSATGQERTQATVGESQRKRGRTPAEKENKRLKRLLRNRVSAQQARERKKAYLSELENRVKDLENKNSELEERLSTLQNENQMLRHILKNTTGNKRGGGGGSNADASL hy5-dna ESMA reveals interaction between HY5 protein and the Chalcone synthase promoter 729029 N/A 729031 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag mutation analysis mutation analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecobb Escherichia coli (strain B / BL21) N/A 729032 unspecified role unspecified role bait bait direct interaction direct interaction false false false Figure 6 Binding of HY5 to the DNA probe was abolished by the presence of unlabelled synthetic consensus G-box tetramer and the DNA region described by the authors as Unit1 which contains the G box like motif. The DNA probe was 32P labelled. hy5-dna-1 809757 n/a 729031 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 729032 unspecified role unspecified role bait bait direct interaction direct interaction false false false Figure 4B The authors concluded " unphosphorylated HY5 is more effective in binding to these promoters". MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint t_rkeri-2012-1 Phylogenetic and functional features of the plastid transcription kinase cpCK2 from Arabidopsis signify a role of cysteinyl SH-groups in regulatory phosphorylation of plastid sigma factors. in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Türkeri H., Schweer J., Link G. FEBS J. (1742-464X) 2012 o49935_sinal Sig1 protein protein sinal Sinapis alba (White mustard) Brassica hirta White mustard MATTAVIGLNAGKRLLSSSFYYSDVTEKFLSVNDLCSSQYHTRSGITAKKTSSSSSSNYSPSFPSSSRQTQSAKALKESVASSVQPWLPPGSDQELEEDDIDHSVEALLLLQRSMLEKQWNLSFEKTRKKVPVTCSGISARQRRIGAKKKTNVKAVSEVNFQNNLVKGYVKGVISDHVLSHAEVVRLSKKIKSGLRLDEHKSRLTDRLGCEPSDEQLAMSLKISRAELQAWLMECHLAREKLAMSNVRLVMSIAQRYDNMGAEMSDLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVDNSRTLRLPTHLHERLGLIRNAKLRLQEKGITPSIDRIAESLNMSQKKVRNATEAVSKIFSLDRDAFPSLNGLPGETHHSYIADNRLENNPWHGYDALALKDEVSKLISVTLGEREREIIRLYYGLDKECLTWEDISQRIGLSRERVRQVGLVALEKLKHAARKRKMEAMILKN mint csk2p_arath Casein kinase II subunit alpha-4, chloroplastic CKA4 Plastid-targeted casein kinase 2 alpha F21P24.13 At2g23070 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MALRPCTGFTISSLRNASAANNNLFSLLSFSSSSPAKRNLLLSSLQDNLRRFASSASLYRQHLRNQQQQHQQQQQSRVKEKSETLAQKIGKSIRRAGAPSKARVYADVNVVRPKDYWDYESLAVQWGVQDDYEVVRKVGRGKYSEVFEGIHATDNEKCVIKILKPVKKKKIKREIKILQNLCGGPNIVKLLDIVRDQQSKTPSLIFEHVNNKDFKVLYPTLSDYDVRYYIFELLKALDFCHSRGIMHRDVKPHNVMIDHEQRKLRLIDWGLAEFYHPGKEYNVRVASRYFKGPELLVDLQDYDYSLDLWSLGCMFAGMIFRKEPFFYGHDNYDQLVKIAKVLGTDELNAYLNKYRIELDPNLTSLVGRHSRKPWTKFINSENQHLAVPEAVDFVDKLLRYDHQERPTAKEAMAHPYFYPIRNAESSRTPRSQ sigf_arath RNA polymerase sigma factor sigF, chloroplastic SIGF Protein SINGLET OXYGEN-LINKED DEATH ACTIVATOR 8 RNA polymerase sigma factor sig6 SIG6 SOLDAT8 T1J8 At2g36990 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEATRNLVSSSPSFQTKTHLKSSYSSPSSVVMLHDQTTTPVVNSRHLNSLSRHFPASVLSQEPREESRPLSHALRDDRTSQLTLERRQFDELVSSREDEKFEQQLLHSTGLWNLLISPLTSETKLPAVVSPLADAELCDVVALAQKALSASKQAALLVDDTEANPSDNIKDSLSTSSSMSLPEKGNIVRSKRQLERRAKNRRAPKSNDVDDEGYVPQKTSAKKKYKQGADNDDALQLFLWGPETKQLLTAKEEAELISHIQHLLKLEKVKTKLESQNGCEPTIGEWAEAMGISSPVLKSDIHRGRSSREKLITANLRLVVHIAKQYQNRGLNFQDLLQEGSMGLMKSVEKFKPQSGCRFATYAYWWIRQSIRKSIFQNSRTIRLPENVYMLLGKVSEARKTCVQEGNYRPSKEELAGHVGVSTEKLDKLLYNTRTPLSMQQPIWSDQDTTFQEITPDSGIETPTMSVGKQLMRNHVRNLLNVLSPKERRIIKLRFGIDGGKQRSLSEIGEIYGLSKERVRQLESRALYRLKQNMNSHGLHAYADLLV q9mth3_sinal RNA polymerase sigma factor sig2 protein protein sinal Sinapis alba (White mustard) Brassica hirta White mustard MSSCLLPNFKCQPDSFSVHFRTSYSVSKHSKGSAFFQPQCAVSTSPPLLTSTLDVAKLRLPSFDTDSDSRISDRPWTYTRTIGPSTEAKYLEALASETLLTSDEAVVAAAAAEAVALARAALKVAKDATLFGNSHNTKLSVSSAADKRSKWDQFTEKERAGILGHLAVSDTGVVSDRITAPGSLECNKEASDDLTPAEKQEVEKLSVSSAVRSTRQTERKARRVKGLEKTASGMQSSVKTGSSSRKKRVASQEIDHNDPLRYLRMTTSSSKLLTAREEQQLSEGIQDLLKLERLQAELTERCGHQPTFSQWASAAGVDKKTLRKRITHGTQCKDRMIKSNIRLVISIAKNYQGAGMDFQDLVQEGCRGLVRGAEKFDATKGFKFSTYAHWWIKQAVRKSLSDQSRMIRLPFHMVEATYRVKEARKQLYSETGKQPKNEEVAEATGLSMKRLMAVLLSPKPPRSLDQKIGINLNLKPSEVIADPEAETSEDILMKQFMRQDLDKVLDSLSTREKQVIRWRFGMEDGRMKTLQEIGETMGVSRERVRQIDTSAFRKLKNKKRNSHLQQYLVAQTKES mint siga_arath RNA polymerase sigma factor sigA SIGA RNA polymerase sigma factor sig1 RNA polymerase sigma factor sig2 RNA polymerase sigma factor sigB RPOD1 SIG1 SIG2 SIGB F13O11.16 At1g64860 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATAAVIGLNTGKRLLSSSFYHSDVTEKFLSVNDHCSSQYHIASTKSGITAKKASNYSPSFPSSNRHTQSAKALKESVDVASTEKPWLPNGTDKELEEECYDDDDLISHSVEAILLLQKSMLEKSWNLSFEKAVSSEYPGKGTIRKKKIPVITCSGISARQRRIGAKKKTNMTHVKAVSDVSSGKQVRGYVKGVISEDVLSHVEVVRLSKKIKSGLRLDDHKSRLKDRLGCEPSDEQLAVSLKISRAELQAWLMECHLAREKLAMSNVRLVMSIAQRYDNLGAEMSDLVQGGLIGLLRGIEKFDSSKGFRISTYVYWWIRQGVSRALVDNSRTLRLPTHLHERLGLIRNAKLRLQEKGITPSIDRIAESLNMSQKKVRNATEAVSKVFSLDRDAFPSLNGLPGETHHSYIADTRLENNPWHGYDDLALKEEVSKLISATLGEREKEIIRLYYGLDKECLTWEDISKRIGLSRERVRQVGLVALEKLKHAARKRKMEAMILKN csk2p-sig1 1241344 sig1 SaSig1 1241346 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra enzyme target enzyme target substrate substrate neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false casein kinase 2 cpCK2 1241347 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phosphorylation phosphorylation reaction phosphorylation false false false f2b mint csk2p-sig2 1241344 sig2 SaSig2 1241348 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra enzyme target enzyme target substrate substrate neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false casein kinase 2 cpCK2 1241347 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phosphorylation phosphorylation reaction phosphorylation false false false f2b mint csk2p-siga 1241344 casein kinase 2 cpCK2 1241347 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false sig1 AtSig1 1241349 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra enzyme target enzyme target substrate substrate neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phosphorylation phosphorylation reaction phosphorylation false false false f2b mint csk2p-sigf 1241344 casein kinase 2 cpCK2 1241347 predetermined predetermined participant predetermined enzyme enzyme neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false sig6 AtSig6 1241350 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra enzyme target enzyme target substrate substrate neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false phosphorylation phosphorylation reaction phosphorylation false false false f2b mint