mpidb The Microbial Protein Interaction Database rincon-2007-1 A novel cell surface-anchored cellulose-binding protein encoded by the sca gene cluster of Ruminococcus flavefaciens. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Rincon M.T., Cepeljnik T., Martin J.C., Barak Y., Lamed R., Bayer E.A., Flint H.J. J. Bacteriol. (0021-9193) 2007 Only protein-protein interactions imex curation q9ae52_rumfl scaB protein protein ruminococcus flavefa Ruminococcus flavefaciens MKKLLSAVTSVAMSFTLMAGAFASSVSAAGGLSAVQPNVSLGEVLDVSANRTAADGTVEWLIPTVTAAPGQTVTMPVVVKSSSLAVAGAQFKIQAATGVRYSSKTDGDAYGSGIVYNNSKYAFGQGAGRGIVAADDSVVLTLAYTVPADCAEGTYDVKWSDAFVSDTDGQNITSKVTLTDGAIIVKKDNNTGKIKWVLDKVTAAPGETVTVKATVDASGEGTVAVAGAQFDVNADSPIVYDSAKEGGAYITSLTVNDAKHKFAFGRTTGTGIVAADKATVAAFTYKVPSDCTEGTYDVKWSGGFVSDADGKDITSRVTFVDGSITVKNDVQTVGKWVLDNVTAAQGETVTLKAVVDDSAKAAVPVAGAQFKLQAADAVKYSSFTDGTAYGAAFTKNDDKQSFAFGNTGGKGVAAADKAAIYTVSYTVPTDCPDGTYPVKWADTFVSDTDGRNITSNIIFVDGSITVGKPVTGTVEWVIPKVDADAGDNVTLQVAVKGDADLPVSAAQFDVNGATPLNYQESAGKPYGVDLVVNDTKQHFAFAQASGISKTAKDGDKLFTITFKVPEDCADGIYPVTWAKQFVSDENGNNITDKVTFTDGWVRVGNPPTTTTTTTTSVTTTTTTTTVTAPKGGIIWAIDTVTVEPGQTATLNIKVVDEDNSKLPIAGAQFNIVNKDGVNYSSIAGSEAYGPTITANDAKKKFDFANASGVETVGANNANVAVLTYTVPATAEEGVYPVQFAEGSLKIFDKDGKDISDKIIGVDGAIIVKKPVVTDVTSTTTVTSNVNTTTSTTTVTAPQGGIIWAIDTVTVEPGQTATLNIKVVDLDNSQLPIAGAQFNIVNKDGVNYSSIAGSEAYGPKITANDAKKKFDFANASGVETVGANDAIVAVLTYTVPATAEEGVYPVQFAEGSLKIFDKDGKDISDKIIGVDGAIIVKKPVITETSTTTTVTGKPNTSTTTTTGTDVTTSGNETTDTTVTTVTTTDVTGPTTTSTDTTVTSSVVGPTTTSTIVTDTTVTSSVIGPTTTTTVVTDTTVTSSVIGPTTTTTVVTDTTVTSSVIGPTTTTTVVTDTTVTSSVIGPTTTTTVVTDTTVTSSVIGPTTTSTTVTTEVTGPTDTTATSTGTSTTDVTGPTDTTVTSTGTSTTDVTGPTDTTVTSTGTSTTDVTGPTDTTVTSTSTTTDVTGPTDTTVTDTTVTTVVTNPTTTLTTTTIVVPKDSIAWVVDTVAAYPGDEVTLKITVKDPNAANLGIAGAQFKVTPDGDVQLVKSGDKSGYGADIIPSKDGYKFVFADGAGVAHVAPDGTVVIELTYKVGEEVAPGTVIPVSIKDLYVVDEDGNDITKYVMPVDGSIIVFEKPAPTSTTTNVTVTTVTTDVTGPTDTTVTSTGTSTTDVTGPTDTTVTSTGTSTTDVTGPTDTTVTSTSTTTDVTGPTDTTVTSTSTTTDVTGPTDTTVTSTSTTTDVTGPTDTTETSTSTTTDVTGPTTTSTTSTSTETTSTDTTTSTSTSSTSTTSTTDANYDHSYAVIETQVGYYFSHDDGVRANGQVGGFNKGQVVTLKIVDVYKDGSEVERPEVNMDLINFNNNTPEKAYNSRTHINKQTTITDFKYDIPVYYGDMPLVDKSGKALTVTAYIGVKGDITLSNMVDSNDASVALSYYTLISTGSNTPANTICQITEEGLQVSSALDELDNLAAFLGDVNTNEWDPDNWKTTKMGRKIDSNDASQILAFYSKRTSHVYDDVSSNDIWNEILGDARFGK q4a3y2_rumfl scaE protein protein ruminococcus flavefa Ruminococcus flavefaciens MKKNIFKSAFAALAVSAVALSASAVSAFAGPAAGQAYDAGNLDVASSPVKPTLSITKKTLTAAEAPNAKVTMELSVEGAADKYAATGLHIQFDPKLKLIPDEDGALATAGRAARLLELKKAEADTDNSFFTATGSSTNNGKDGVLWSFVLQVPADAQPGDKYDVQVAYQSRTTNEDLFTNVKKDEEGLLMQAWTFTQGIEQGYIQVESTTSSTTTTTTTTTTTTTTSTTSTTSTTSTTSAAGTTARATSAAGTNTAKPAVTTTKAKATTTTKKGDSPKTGVAGAGVAVAGLAIAVGTAFALRKKED q4a3y3_rumfl cttA protein protein ruminococcus flavefa Ruminococcus flavefaciens MLERYRTMKNSLFKRAIATAAAVPLALTQCLTYANAVSTDFVKAPAQNVQEGESLKLENLLYIPAEETVSTWNQTLSAAIASIGAEDGEVDVAKFRDDILSKAGAYKEAAAYALDQCIIPNKVNYEITGENDVVITGKISEPDLNLKIANTPADALHRIADSYNAPALKNVDYSTVKIGGDFKITIGTSALASGKKVAVTFAYTTADGTYGIGELPAYAIKKIEEIRKIDEAAIEKEVAADKVDEAKAKYNAKIDLIIDKLKKAERAVDKTLKADKNATYSDVSGLISDTNGWLKANGINRQLPGTASEIAAKEFIVKAYDTAIKTIDSKGVVDISAADLGGFADSIKNVTFSLANGKAEGIGTFDDAEQAEVKAWVEKNGYAFVDSYKKITAKVDFSGIKSVDAGSVEVEIERILVTDTTTTTQVTTTSTETTSTTSTESTSTTTEETTTTDTTSTTTETTSTSTETTSTTSETTSTSTTSETTSTTSETTSTTSETTSTSTDTTSTSTETTPVVTTVFTKVYATANTENAFYLNTETEFDKAQLSNVILHTVRIEAAVKEDGTIDETTKKETEIEAKDITADVSFGSATPANTYEKTNTSFKYKVPVSANGSVLKDVDGNDFTVTVFIGVKGDINLDNMANSVDASQALAFYAKTSTQGRGVYDVQLSESNLVLTPKSVYDEFAAFLGDVHHVSGAVVDRFATKDKRLIDANDASNILAFYAKRMNSANDQMTDAEIWEDILA scae-ctta 1331825 not-defined-546439915 1331826 unspecified role unspecified role bait bait cbm fusion fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-2051256402 1331827 unspecified role unspecified role prey prey xylanase fusion fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false figure 5 scae-scab 1331825 n/a 1331828 unspecified role unspecified role prey prey xylanase fusion fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli not-defined-546439915 1331826 unspecified role unspecified role bait bait cbm fusion fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false figure 5 MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint karpol-2013-1 Structural and functional characterization of a novel type-III dockerin from Ruminococcus flavefaciens. in vitro In vitro itc isothermal titration calorimetry ITC ITC itc experimental particp experimental participant identification experimental particp Karpol A., Jobby MK., Slutzki M., Noach I., Chitayat S., Smith SP., Bayer EA. FEBS Lett. (0014-5793) 2013 karpol-2013-2 Structural and functional characterization of a novel type-III dockerin from Ruminococcus flavefaciens. in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column experimental particp experimental participant identification experimental particp Karpol A., Jobby MK., Slutzki M., Noach I., Chitayat S., Smith SP., Bayer EA. FEBS Lett. (0014-5793) 2013 karpol-2013-3 Structural and functional characterization of a novel type-III dockerin from Ruminococcus flavefaciens. in vitro In vitro biophysical biophysical experimental particp experimental participant identification experimental particp Karpol A., Jobby MK., Slutzki M., Noach I., Chitayat S., Smith SP., Bayer EA. FEBS Lett. (0014-5793) 2013 karpol-2013-4 Structural and functional characterization of a novel type-III dockerin from Ruminococcus flavefaciens. in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa experimental particp experimental participant identification experimental particp Karpol A., Jobby MK., Slutzki M., Noach I., Chitayat S., Smith SP., Bayer EA. FEBS Lett. (0014-5793) 2013 voronov_goldman-2015-2 in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa elisa enzyme linked immunosorbent assay ELISA ELISA elisa Voronov-Goldman M., Yaniv O., Gul O., Yoffe H., Salama-Alber O., Slutzki M., Levy-Assaraf M., Jindou S., Shimon LJ., Borovok I., Bayer EA., Lamed R., Frolow F., Voronov-Goldman M., Yaniv O., Gul O., Yoffe H., Salama-Alber O., Slutzki M., Levy-Assaraf M., Jindou S., Shimon LJ., Borovok I., Bayer EA., Lamed R., Frolow F. ed.bayer@weizmann.ac.il FEBS letters 2015 Only protein-protein interactions imex curation imex curation voronov_goldman-2015-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Voronov-Goldman M., Yaniv O., Gul O., Yoffe H., Salama-Alber O., Slutzki M., Levy-Assaraf M., Jindou S., Shimon LJ., Borovok I., Bayer EA., Lamed R., Frolow F., Voronov-Goldman M., Yaniv O., Gul O., Yoffe H., Salama-Alber O., Slutzki M., Levy-Assaraf M., Jindou S., Shimon LJ., Borovok I., Bayer EA., Lamed R., Frolow F. ed.bayer@weizmann.ac.il FEBS letters 2015 Only protein-protein interactions imex curation imex curation q9ae53_rumfl scaA protein protein ruminococcus flavefa Ruminococcus flavefaciens MKKLISAVTSLCMAATMVSVVAPSTVGAADTAKGFAVKTYDADKPSSADGKSTVTIDKKDIPASGYTFPAALYYSEESASTTSFLVHLTTDSSAITFPKVYEPDQPYFSAEKEQVMASGTASTDCYVSFGGQLSKRGKYSAAGLAKFGLDSSQSAAGTDNYFLGCSWMNMGEEYTWAGAKSDSFPVYVFDVNIPATIAAGTYHITYCKYDTDPDPANEVMAPMAEAKTNDVKSKYNLASNNLKLEEMTIVVTENGSTPGTTTTVTQPPVTTTTTVGTVTPGDKDFNFKFVDDKGNSVVSANPGDEITVCVQVDAGNNTCAGMDVKFNTSGLAIDEFENNSEACGNAKLAKNEKEFRANFTSTGTDGEPMKVSNGKDAFTFYVTIPATAKDTSYTIGFVDGELNVFKEGGTGSKYTAGYTPLTINVGTPNPGTTTTVTQPPVTTTTTGGQTSNADFKFSFVDDKGQSTVNAKAGDEITVCVQVDAGNNTCAGMDVQFSTSGLAIDEFENNSEACGNAKLAKNEKELRANFTSTGTDGEPMKVSNGKDAFTFYVTIPAYAKDTYYVIGFVDSELNVFKEGGTGDNTIAFYTPLTINGTPNPGTTSTVTQPPVTTTTTGGQTSNAEFKFSFVDDKDQSAINVNAGDEVTVCVKVDAGNNTCAGMDVQFSTSGLAIDEFENNSEACGNAKLAKNEKELRANFTSTGTDGEPMKVSNGKDAFTFYVTIPATAKDGDTYKIGFVDSELKVFKEGGTGDKYTASYTPLTITVGKQPVTTSTTTTTVTTTSTTTTTVTTPQPGTKLVPTWGDTNCDGVVNVADVVVLNRFLNDPTYSNITDQGKVNADVVDPQDKSGAAVDPAGVKLTVADSEAILKAIVELITLPQ mint q9ae52_rumfl scaB protein protein ruminococcus flavefa Ruminococcus flavefaciens MKKLLSAVTSVAMSFTLMAGAFASSVSAAGGLSAVQPNVSLGEVLDVSANRTAADGTVEWLIPTVTAAPGQTVTMPVVVKSSSLAVAGAQFKIQAATGVRYSSKTDGDAYGSGIVYNNSKYAFGQGAGRGIVAADDSVVLTLAYTVPADCAEGTYDVKWSDAFVSDTDGQNITSKVTLTDGAIIVKKDNNTGKIKWVLDKVTAAPGETVTVKATVDASGEGTVAVAGAQFDVNADSPIVYDSAKEGGAYITSLTVNDAKHKFAFGRTTGTGIVAADKATVAAFTYKVPSDCTEGTYDVKWSGGFVSDADGKDITSRVTFVDGSITVKNDVQTVGKWVLDNVTAAQGETVTLKAVVDDSAKAAVPVAGAQFKLQAADAVKYSSFTDGTAYGAAFTKNDDKQSFAFGNTGGKGVAAADKAAIYTVSYTVPTDCPDGTYPVKWADTFVSDTDGRNITSNIIFVDGSITVGKPVTGTVEWVIPKVDADAGDNVTLQVAVKGDADLPVSAAQFDVNGATPLNYQESAGKPYGVDLVVNDTKQHFAFAQASGISKTAKDGDKLFTITFKVPEDCADGIYPVTWAKQFVSDENGNNITDKVTFTDGWVRVGNPPTTTTTTTTSVTTTTTTTTVTAPKGGIIWAIDTVTVEPGQTATLNIKVVDEDNSKLPIAGAQFNIVNKDGVNYSSIAGSEAYGPTITANDAKKKFDFANASGVETVGANNANVAVLTYTVPATAEEGVYPVQFAEGSLKIFDKDGKDISDKIIGVDGAIIVKKPVVTDVTSTTTVTSNVNTTTSTTTVTAPQGGIIWAIDTVTVEPGQTATLNIKVVDLDNSQLPIAGAQFNIVNKDGVNYSSIAGSEAYGPKITANDAKKKFDFANASGVETVGANDAIVAVLTYTVPATAEEGVYPVQFAEGSLKIFDKDGKDISDKIIGVDGAIIVKKPVITETSTTTTVTGKPNTSTTTTTGTDVTTSGNETTDTTVTTVTTTDVTGPTTTSTDTTVTSSVVGPTTTSTIVTDTTVTSSVIGPTTTTTVVTDTTVTSSVIGPTTTTTVVTDTTVTSSVIGPTTTTTVVTDTTVTSSVIGPTTTTTVVTDTTVTSSVIGPTTTSTTVTTEVTGPTDTTATSTGTSTTDVTGPTDTTVTSTGTSTTDVTGPTDTTVTSTGTSTTDVTGPTDTTVTSTSTTTDVTGPTDTTVTDTTVTTVVTNPTTTLTTTTIVVPKDSIAWVVDTVAAYPGDEVTLKITVKDPNAANLGIAGAQFKVTPDGDVQLVKSGDKSGYGADIIPSKDGYKFVFADGAGVAHVAPDGTVVIELTYKVGEEVAPGTVIPVSIKDLYVVDEDGNDITKYVMPVDGSIIVFEKPAPTSTTTNVTVTTVTTDVTGPTDTTVTSTGTSTTDVTGPTDTTVTSTGTSTTDVTGPTDTTVTSTSTTTDVTGPTDTTVTSTSTTTDVTGPTDTTVTSTSTTTDVTGPTDTTETSTSTTTDVTGPTTTSTTSTSTETTSTDTTTSTSTSSTSTTSTTDANYDHSYAVIETQVGYYFSHDDGVRANGQVGGFNKGQVVTLKIVDVYKDGSEVERPEVNMDLINFNNNTPEKAYNSRTHINKQTTITDFKYDIPVYYGDMPLVDKSGKALTVTAYIGVKGDITLSNMVDSNDASVALSYYTLISTGSNTPANTICQITEEGLQVSSALDELDNLAAFLGDVNTNEWDPDNWKTTKMGRKIDSNDASQILAFYSKRTSHVYDDVSSNDIWNEILGDARFGK w5idc3_rumfl protein protein ruminococcus flavefa Ruminococcus flavefaciens MGSSSVTADLNNAVINVDEMNEAFKDVPDLEGEGAHITLSNTTAKPGEMAEVTMSVSNADMQWNMCGIHIIYPDILKPEMKDPEERTVAFQKGDALEAATGIVCMEWQEGLPPVLTENKKGCLFLTAMFSGNQGGEGDMATFRFKVPDNAEPGAVYNLGYYYMNTDLFINEQNIPTYQKYAFTHMEGGTITVELEHHHHHH a0aef5_rumfl cttA protein protein ruminococcus flavefa Ruminococcus flavefaciens MKNSLFKRVAAAAAAVPLALTQCLTFSSVAAENDAVQIVGNTVQDGAAQTVSLESLLYIPANKTESTWNVTGSAALAKMVGTKGSINNAKILDLVKYVPANYREAAKAALEEYVLVNPVTYEVTADKNIVLKATVSQPNFNGNWANTPGKALSDLAKAYNAPELNAVDFSSVKVGGDLVVTIKTSKLDEGTTVPVEVVYKTADGDIYAGDFAKFANDKLTELENIAKKAIENDVAAQYAKEATDKFLAKTALIRDKINKAEKALDQALTKTADYKSISEAADAANKFLAKKNINKKVPSSAAEVAAKETVKKYYDAAVKKASAKADIQITVEQLGAFVDSLTSKTLTDSEGNEFATAFDINLNNGKATLVGAFEDAEAADVQKWVESKGYAYVGSYKKLTGTVDFTGIKTADTASVDVQIERILVTETTTTTTTTTDDGSTTTTTTDDGSTTTTTTDDGSTTTTTSTTDDGSTTSTTSTTDDGSTTSTTSTTDDGSTTSTTSTTDDGSTTSTTSTTDDGSTTSTTSTTDDGSTTSTTSTTDDGSTTSTTSTTGDGSTTTTTTTTNTVTSAVKTQYVEIESVDGFYFNTEDKFDTAQIKKAVLHTVYNEGYTGDDGVAVVLREYESEPVDITAELTFGDATPANTYKAVENKFDYEIPVYYNNATLKDAEGNDATVTVYIGLKGDTDLNNIVDGRDATATLTYYAATSTDGKDATTVALSPSTLVGGNPESVYDDFSAFLSDVKVDAGKELTRFAKKAERLIDGRDASSILTFYTKSSVDQYKDMAANEPNKLWDIVTGDAEEE a0aef6_rumfl scaE protein protein ruminococcus flavefa Ruminococcus flavefaciens MKKNTLKKAISAVAVSALALTASSVSAFALTDRGMTYDLDPKDGSSAATKPVLEVTKKVFDTAADAAGQTVTVEFKVSGAEGKYATTGYHIYWDERLEVVATKTGAYAKKGAALEDSSLAKAENNGNGVFVASGADDDFGADGVMWTVELKVPADAKAGDVYPIDVAYQWDPSKGDLFTDNKDSAQGKLMQAYFFTQGIKSSSNPSTDEYLVKANATYADGYIAIKAGEPETTTTTTTTTTTTTTTTTTTTTTTSTSSTTTTTGSTTSANAESTTGAAQSTGAQTTTAKSNSSDNKKTDSPKTGVTGVGVAVAGLAVAVGTAFALKKKED scaa-scab 1328248 q9ae53_rumfl ScaADoc 1328252 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.1 scab ScaB cohesin 1328253 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.1 direct interaction direct interaction false false false f5b mint scaa-scab-1 1328249 q9ae53_rumfl ScaADoc 1328252 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.1 scab ScaB cohesin 1328253 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.1 direct interaction direct interaction false false false f5a mint scaa-scab-2 1328250 q9ae53_rumfl ScaADoc 1328252 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false scab ScaB cohesin 1328253 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f6a mint scab-scaa 1328251 q9ae53_rumfl ScaADoc 1328252 predetermined predetermined participant predetermined unspecified role unspecified role prey prey purified purified fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false scab ScaB cohesin 1328253 predetermined predetermined participant predetermined unspecified role unspecified role bait bait purified purified fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false f6b mint ctta-w5idc3_rumfl-1 1870786 N/A 1870788 unspecified role unspecified role prey prey purified purified cmb tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1870789 unspecified role unspecified role bait bait purified purified direct interaction direct interaction false false false f6 scae-w5idc3_rumfl-1 1870786 N/A 1870789 unspecified role unspecified role bait bait purified purified N/A 1870790 unspecified role unspecified role prey prey purified purified cmb tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false direct interaction direct interaction false false false f6 1-56 1870787 N/A 1870788 unspecified role unspecified role neutral component neutral component purified purified N/A 1870789 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false direct interaction direct interaction false false false t1 f2 f4