DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ macho-2014-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. DIP original experiment: DIP-266323X Science (New York, N.Y.) 2014 IMEx A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. Only protein-protein interactions imex curation macho-2014-4 nicbe Nicotiana benthamiana anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. DIP original experiment: DIP-266321X Science (New York, N.Y.) 2014 IMEx A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. Only protein-protein interactions imex curation macho-2014-5 in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. DIP original experiment: DIP-266319X Science (New York, N.Y.) 2014 IMEx A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. Only protein-protein interactions imex curation macho-2014-6 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. DIP original experiment: DIP-266322X Science (New York, N.Y.) 2014 IMEx A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Macho AP., Schwessinger B., Ntoukakis V., Brutus A., Segonzac C., Roy S., Kadota Y., Oh MH., Sklenar J., Derbyshire P., Lozano-Durán R., Malinovsky FG., Monaghan J., Menke FL., Huber SC., He SY., Zipfel C. Only protein-protein interactions imex curation abramovitch-2006-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Abramovitch RB., Janjusevic R., Stebbins CE., Martin GB. DIP original experiment: DIP-262593X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Type III effector AvrPtoB requires intrinsic E3 ubiquitin ligase activity to suppress plant cell death and immunity. Abramovitch RB., Janjusevic R., Stebbins CE., Martin GB. Only protein-protein interactions imex curation abramovitch-2006-2 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Abramovitch RB., Janjusevic R., Stebbins CE., Martin GB. DIP original experiment: DIP-262596X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Type III effector AvrPtoB requires intrinsic E3 ubiquitin ligase activity to suppress plant cell death and immunity. Abramovitch RB., Janjusevic R., Stebbins CE., Martin GB. Only protein-protein interactions imex curation abramovitch-2006-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Abramovitch RB., Janjusevic R., Stebbins CE., Martin GB. DIP original experiment: DIP-262594X Proceedings of the National Academy of Sciences of the United States of America 2006 IMEx Type III effector AvrPtoB requires intrinsic E3 ubiquitin ligase activity to suppress plant cell death and immunity. Abramovitch RB., Janjusevic R., Stebbins CE., Martin GB. Only protein-protein interactions imex curation rosebrock-2007-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rosebrock TR., Zeng L., Brady JJ., Abramovitch RB., Xiao F., Martin GB. DIP original experiment: DIP-253181X Nature 2007 IMEx A bacterial E3 ubiquitin ligase targets a host protein kinase to disrupt plant immunity. Rosebrock TR., Zeng L., Brady JJ., Abramovitch RB., Xiao F., Martin GB. Only protein-protein interactions imex curation rosebrock-2007-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rosebrock TR., Zeng L., Brady JJ., Abramovitch RB., Xiao F., Martin GB. DIP original experiment: DIP-253179X Nature 2007 IMEx A bacterial E3 ubiquitin ligase targets a host protein kinase to disrupt plant immunity. Rosebrock TR., Zeng L., Brady JJ., Abramovitch RB., Xiao F., Martin GB. Only protein-protein interactions imex curation rosebrock-2007-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rosebrock TR., Zeng L., Brady JJ., Abramovitch RB., Xiao F., Martin GB. DIP original experiment: DIP-253180X Nature 2007 IMEx A bacterial E3 ubiquitin ligase targets a host protein kinase to disrupt plant immunity. Rosebrock TR., Zeng L., Brady JJ., Abramovitch RB., Xiao F., Martin GB. Only protein-protein interactions imex curation wilton-2010-1 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Wilton M., Subramaniam R., Elmore J., Felsensteiner C., Coaker G., Desveaux D. DIP original experiment: DIP-219216X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The type III effector HopF2Pto targets Arabidopsis RIN4 protein to promote Pseudomonas syringae virulence. Wilton M., Subramaniam R., Elmore J., Felsensteiner C., Coaker G., Desveaux D. Only protein-protein interactions imex curation wilton-2010-2 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Wilton M., Subramaniam R., Elmore J., Felsensteiner C., Coaker G., Desveaux D. DIP original experiment: DIP-219218X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The type III effector HopF2Pto targets Arabidopsis RIN4 protein to promote Pseudomonas syringae virulence. Wilton M., Subramaniam R., Elmore J., Felsensteiner C., Coaker G., Desveaux D. Only protein-protein interactions imex curation wilton-2010-3 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wilton M., Subramaniam R., Elmore J., Felsensteiner C., Coaker G., Desveaux D. DIP original experiment: DIP-219217X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx The type III effector HopF2Pto targets Arabidopsis RIN4 protein to promote Pseudomonas syringae virulence. Wilton M., Subramaniam R., Elmore J., Felsensteiner C., Coaker G., Desveaux D. Only protein-protein interactions imex curation efr_arath LRR receptor-like serine/threonine-protein kinase EFR EFR Elongation factor Tu receptor F7C8.70 At5g20480 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKLSFSLVFNALTLLLQVCIFAQARFSNETDMQALLEFKSQVSENNKREVLASWNHSSPFCNWIGVTCGRRRERVISLNLGGFKLTGVISPSIGNLSFLRLLNLADNSFGSTIPQKVGRLFRLQYLNMSYNLLEGRIPSSLSNCSRLSTVDLSSNHLGHGVPSELGSLSKLAILDLSKNNLTGNFPASLGNLTSLQKLDFAYNQMRGEIPDEVARLTQMVFFQIALNSFSGGFPPALYNISSLESLSLADNSFSGNLRADFGYLLPNLRRLLLGTNQFTGAIPKTLANISSLERFDISSNYLSGSIPLSFGKLRNLWWLGIRNNSLGNNSSSGLEFIGAVANCTQLEYLDVGYNRLGGELPASIANLSTTLTSLFLGQNLISGTIPHDIGNLVSLQELSLETNMLSGELPVSFGKLLNLQVVDLYSNAISGEIPSYFGNMTRLQKLHLNSNSFHGRIPQSLGRCRYLLDLWMDTNRLNGTIPQEILQIPSLAYIDLSNNFLTGHFPEEVGKLELLVGLGASYNKLSGKMPQAIGGCLSMEFLFMQGNSFDGAIPDISRLVSLKNVDFSNNNLSGRIPRYLASLPSLRNLNLSMNKFEGRVPTTGVFRNATAVSVFGNTNICGGVREMQLKPCIVQASPRKRKPLSVRKKVVSGICIGIASLLLIIIVASLCWFMKRKKKNNASDGNPSDSTTLGMFHEKVSYEELHSATSRFSSTNLIGSGNFGNVFKGLLGPENKLVAVKVLNLLKHGATKSFMAECETFKGIRHRNLVKLITVCSSLDSEGNDFRALVYEFMPKGSLDMWLQLEDLERVNDHSRSLTPAEKLNIAIDVASALEYLHVHCHDPVAHCDIKPSNILLDDDLTAHVSDFGLAQLLYKYDRESFLNQFSSAGVRGTIGYAAPEYGMGGQPSIQGDVYSFGILLLEMFSGKKPTDESFAGDYNLHSYTKSILSGCTSSGGSNAIDEGLRLVLQVGIKCSEEYPRDRMRTDEAVRELISIRSKFFSSKTTITESPRDAPQSSPQEWMLNTDMHTM hopd2_psesm Effector protein hopD2 hopD2 hopA01 hopPtoD2 PSPTO_4722 Tyrosine-protein phosphatase hopPtoD2 protein protein psesm Pseudomonas syringae pv. tomato (strain DC3000) MNPLQPIQHSITNSQMSGGQQLEAEGSQAHNSYSHPDRISLSQLSQSAHLALDHLSTQPNTDHQRVASLVRNAVQDGKFQLQSSNDTQVTYKTSVCPPANADTMGAAHLINNELTVQARLNDQLEYDIVSAHLYGPSEAISIDASSPPSANDLASSGLSERTHLGMNRVLLRYAVPPRETEDQCVMVIDKMPPPKHGKMSFFRTTNDLSKLPLGMETGGLSDLKLAGCERISSVEQVKSIRAALGGGPLTVLDLREESHAIVNGLPITLRGPMDWANAGLSQVDGAARESAMITELKRTKSLTLVDANYVKGKKSNPQTTELKNLNVRSEREVVTEAGATYRRVAITDHNRPSPEATDELVDIMRHCLQANESLVVHCNGGRGRTTTAMIMVDMLKNARNHSAETLITRMAKLSYDYNMTDLGSISALKRPFLEDRLKFLQAFHDYARNNPSGLSLNWTQWRAKIALE fls2_arath LRR receptor-like serine/threonine-protein kinase FLS2 FLS2 Protein FLAGELLIN-SENSING 2 Protein FLAGELLIN-SENSITIVE 2 MPL12.13 MPL12.8 At5g46330 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKLLSKTFLILTLTFFFFGIALAKQSFEPEIEALKSFKNGISNDPLGVLSDWTIIGSLRHCNWTGITCDSTGHVVSVSLLEKQLEGVLSPAIANLTYLQVLDLTSNSFTGKIPAEIGKLTELNQLILYLNYFSGSIPSGIWELKNIFYLDLRNNLLSGDVPEEICKTSSLVLIGFDYNNLTGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDLSGNQLTGKIPRDFGNLLNLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIPAELGNLVQLQALRIYKNKLTSSIPSSLFRLTQLTHLGLSENHLVGPISEEIGFLESLEVLTLHSNNFTGEFPQSITNLRNLTVLTVGFNNISGELPADLGLLTNLRNLSAHDNLLTGPIPSSISNCTGLKLLDLSHNQMTGEIPRGFGRMNLTFISIGRNHFTGEIPDDIFNCSNLETLSVADNNLTGTLKPLIGKLQKLRILQVSYNSLTGPIPREIGNLKDLNILYLHSNGFTGRIPREMSNLTLLQGLRMYSNDLEGPIPEEMFDMKLLSVLDLSNNKFSGQIPALFSKLESLTYLSLQGNKFNGSIPASLKSLSLLNTFDISDNLLTGTIPGELLASLKNMQLYLNFSNNLLTGTIPKELGKLEMVQEIDLSNNLFSGSIPRSLQACKNVFTLDFSQNNLSGHIPDEVFQGMDMIISLNLSRNSFSGEIPQSFGNMTHLVSLDLSSNNLTGEIPESLANLSTLKHLKLASNNLKGHVPESGVFKNINASDLMGNTDLCGSKKPLKPCTIKQKSSHFSKRTRVILIILGSAAALLLVLLLVLILTCCKKKEKKIENSSESSLPDLDSALKLKRFEPKELEQATDSFNSANIIGSSSLSTVYKGQLEDGTVIAVKVLNLKEFSAESDKWFYTEAKTLSQLKHRNLVKILGFAWESGKTKALVLPFMENGNLEDTIHGSAAPIGSLLEKIDLCVHIASGIDYLHSGYGFPIVHCDLKPANILLDSDRVAHVSDFGTARILGFREDGSTTASTSAFEGTIGYLAPEFAYMRKVTTKADVFSFGIIMMELMTKQRPTSLNDEDSQDMTLRQLVEKSIGNGRKGMVRVLDMELGDSIVSLKQEEAIEDFLKLCLFCTSSRPEDRPDMNEILTHLMKLRGKANSFREDRNEDREV rin4_arath RPM1-interacting protein 4 RIN4 MJL12_1 At3g25070 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MARSNVPKFGNWEAEENVPYTAYFDKARKTRAPGSKIMNPNDPEYNSDSQSQAPPHPPSSRTKPEQVDTVRRSREHMRSREESELKQFGDAGGSSNEAANKRQGRASQNNSYDNKSPLHKNSYDGTGKSRPKPTNLRADESPEKVTVVPKFGDWDENNPSSADGYTHIFNKVREERSSGANVSGSSRTPTHQSSRNPNNTSSCCCFGFGGK q88a90_psesm hopF2 PSPTO_0502 hopF protein protein psesm Pseudomonas syringae pv. tomato (strain DC3000) MGNICGTSGSRHVYSPSHTQRITSAPSTSTHVGGDTLTSIHQLSHSQREQFLNMHDPMRVMGLDHDTELFRTTDSRYIKNDKLAGNPQSMASILMHEELRPNRFASHTGAQPHEARAYVPKRIKATDLGVPSLNVMTGSLARDGIRAYDHMSDNQVSVKMRLGDFLERGGKVYADASSVADDGETSQALIVTLPKGQKVPVERV hpab2_psesm Effector protein HopAB2 hopAB2 avrPtoB PSPTO_3087 Avirulence protein AvrPtoB protein protein psesm Pseudomonas syringae pv. tomato (strain DC3000) MAGINRAGPSGAYFVGHTDPEPVSGQAHGSGSGASSSNSPQVQPRPSNTPPSNAPAPPPTGRERLSRSTALSRQTREWLEQGMPTAEDASVRRRPQVTADAATPRAEARRTPEATADASAPRRGAVAHANSIVQQLVSEGADISHTRNMLRNAMNGDAVAFSRVEQNIFRQHFPNMPMHGISRDSELAIELRGALRRAVHQQAASAPVRSPTPTPASPAASSSGSSQRSLFGRFARLMAPNQGRSSNTAASQTPVDRSPPRVNQRPIRVDRAAMRNRGNDEADAALRGLVQQGVNLEHLRTALERHVMQRLPIPLDIGSALQNVGINPSIDLGESLVQHPLLNLNVALNRMLGLRPSAERAPRPAVPVAPATASRRPDGTRATRLRVMPEREDYENNVAYGVRLLNLNPGVGVRQAVAAFVTDRAERPAVVANIRAALDPIASQFSQLRTISKADAESEELGFKDAADHHTDDVTHCLFGGELSLSNPDQQVIGLAGNPTDTSQPYSQEGNKDLAFMDMKKLAQFLAGKPEHPMTRETLNAENIAKYAFRIVP rs27a_sollc Ubiquitin-40S ribosomal protein S27a UBI3 RPS27A protein protein sollc Solanum lycopersicum (Tomato) Lycopersicon esculentum Tomato MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGAKKRKKKTYTKPKKIKHKKKKVKLAVLQFYKVDDTGKVQRLRKECPNAECGAGTFMANHFDRHYCGKCGLTYVYNKAGGD This sequence has been withdrawn from Uniprot. 4A85C770FFC26B76 q9lld3_solpi protein protein solpi Solanum pimpinellifolium Currant tomato Lycopersicon pimpinellifolium MGSKYSKATNSISDASNSRYGVPFENYRVPLVDLEEATNNFDDNFFIAEGGFGKVYRGVLRDGTKVALKRHNCDSQQSIEEFRTEIEILSRRSHPHLVSLIGYCDGRNEMILIYDYMENGNLKSHLYGSDLPSMSWEQRLEICIGAARGLHYLHTNGVMHRDVKSSNILLDENFVPKITDFGLSKTRPQLYQTHVSTDVKGTFGYIDPEYFIKGRLTEKSDVYSFGVVLFEVLCARSAIVQSLPSEMVNLAEWAVESHNNGQLEQIIDPNLAAKIRPESLRKFGETAVKCLALSSEDRPSMGDVLWKLEYALRLQESVI q40126_solpi Fen protein protein solpi Solanum pimpinellifolium Currant tomato Lycopersicon pimpinellifolium MGSKYSKATNSINDASNLSYGVPFENYRVPFVDLEEATNNFDDNFFIGEGGFGKVYRGVLRDGTKVALKKHKPESSQGIEEFETEIEILSFCSHPHLVSLIGFCDERNEMILIYDYMENGNLKSHLYGSDLPSMSWEQRLEICIGAARGLHYLHKNAVIHRDVKCTNILLDENFVPKITDFGISKTMPELDQTHLSTVVRGNIGYIAPEYALWGQLTEKSDVYSFGVVLFEVLCARPALDRSEIMSLDDETQKMGQLEQIVDPTIAAKIRPESLRMFGETAIKCLAPSSKNRPSMGDVLWKLEYALCLQEPTIQDDPE q40234_solpi Pto protein protein solpi Solanum pimpinellifolium Currant tomato Lycopersicon pimpinellifolium MGSKYSKATNSINDALSSSYLVPFESYRVPLVDLEEATNNFDHKFLIGHGVFGKVYKGVLRDGAKVALKRRTPESSQGIEEFETEIETLSFCRHPHLVSLIGFCDERNEMILIYKYMENGNLKRHLYGSDLPTMSMSWEQRLEICIGAARGLHYLHTRAIIHRDVKSINILLDENFVPKITDFGISKKGTELDQTHLSTVVKGTLGYIDPEYFIKGRLTEKSDVYSFGVVLFEVLCARSAIVQSLPREMVNLAEWAVESHNNGQLEQIVDPNLADKIRPESLRKFGDTAVKCLALSSEDRPSMGDVLWKLEYALRLQESVI mint fls2-hopd2-1 3263572 N/A 3263578 unspecified role unspecified role bait bait Region 870-1153 Region 870-1153 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3263579 unspecified role unspecified role prey prey Q79LY0:p.Cys378Ser C378S mutation mutation certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false efr-hopd2-1 3263573 N/A 3263579 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false nicbe Nicotiana benthamiana N/A 3263576 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false nicbe Nicotiana benthamiana physical association physical association false false false efr-hopd2-2 3263574 N/A 3263579 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3263576 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 680-1031 Region 680-1031 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pTyr pTyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation false false false efr-hopd2-3 3263575 N/A 3263579 unspecified role unspecified role prey prey Q79LY0:p.Cys378Ser C378S mutation mutation certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3263576 unspecified role unspecified role bait bait Region 712-1001 Region 712-1001 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false hopab2-ub-1 3141577 N/A 3141581 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3141582 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false hopab2-ub-2 3141578 N/A 3141581 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3141582 unspecified role unspecified role bait bait Region 308-553 Region 308-553 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q8RSY1:p.[Lys512Arg;Lys529Arg] K512R/K529R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false hopab2-ub-3 3141579 N/A 3141581 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3141582 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false hopab2-q9lld3_solpi-1 3158976 N/A 3158979 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3141582 unspecified role unspecified role bait bait Region 1-307 Region 1-307 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q8RSY1:p.[Phe479Ala;Phe525Ala;Pro533Ala] F479A/F525A/P533A mutation increasing strength mutation increasing interaction strength mutation increasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8RSY1:p.[Lys512Arg;Lys520Arg;Lys521Arg;Lys529Arg] K512R/K520R/K521R/K529R mutation increasing strength mutation increasing interaction strength mutation increasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false hopab2-fen-1 3158977 N/A 3158981 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3141582 unspecified role unspecified role bait bait Region 1-387 Region 1-387 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q8RSY1:p.[Phe479Ala;Phe525Ala;Pro533Ala] F479A/F525A/P533A mutation increasing strength mutation increasing interaction strength mutation increasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8RSY1:p.[Lys512Arg;Lys520Arg;Lys521Arg;Lys529Arg] K512R/K520R/K521R/K529R mutation increasing strength mutation increasing interaction strength mutation increasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false hopab2-pto-1 3158978 N/A 3158982 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3141582 unspecified role unspecified role bait bait Region 1-307 Region 1-307 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false rin4-hopf2-1 3203908 N/A 3203911 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3203912 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false rin4-hopf2-2 3203909 N/A 3203911 unspecified role unspecified role bait bait arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3203912 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false hopf2-rin4-1 3203910 N/A 3203911 unspecified role unspecified role prey prey arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3203912 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false UniProt UniProt http://www.uniprot.org nomura-2006-1 A bacterial virulence protein suppresses host innate immunity to cause plant disease. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Nomura K., Debroy S., Lee Y.H., Pumplin N., Jones J., He S.Y. AtMIN12 could not be identified in this article hes@msu.edu Science (0036-8075) 2006 Only protein-protein interactions imex curation nomura-2006-2 A bacterial virulence protein suppresses host innate immunity to cause plant disease. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Nomura K., Debroy S., Lee Y.H., Pumplin N., Jones J., He S.Y. AtMIN12 could not be identified in this article hes@msu.edu Science (0036-8075) 2006 Only protein-protein interactions imex curation nomura-2006-3 A bacterial virulence protein suppresses host innate immunity to cause plant disease. tobac-leaf Nicotiana tabacum (tobacco) leaf leaf leaf pull down pull down anti tag western anti tag western blot anti tag western Nomura K., Debroy S., Lee Y.H., Pumplin N., Jones J., He S.Y. AtMIN12 could not be identified in this article hes@msu.edu Science (0036-8075) 2006 Only protein-protein interactions imex curation rd23a_arath Probable ubiquitin receptor RAD23a RAD23A Putative DNA repair protein RAD23-2 RAD23 RAD23-2 RAD23-like protein 2 F3O9.1 At1g16190 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKLTVKTLKGSHFEIRVLPTDTIMAVKKNIEDSQSKDNYPCGQQLLIHNGKVLKDETTLVENKVTEEGFLVVMLSKSKTASSAGPSSTQPTSTTTSTISSTTLAAPSTTQSIAVPASNSTPVQEQPTAQSDTYGQAASTLVSGSSIEQMVQQIMEMGGGSWDKETVTRALRAAYNNPERAVDYLYSGIPETVTIPATNLSGVGSGRELTAPPPSGGPNSSPLDLFPQEAVSDAAGGDLGTLEFLRGNDQFQQLRSMVNSNPQILQPMLQELGKQNPQLLRLIQENQAEFLQLLNEPYEGSDGDVDIFDQPDQEMPHSVNVTPEEQESIERLEAMGFDRAIVIEAFLSCDRNEELAANYLLEHSADFED hopm1_psesm Effector protein HopM1 PSPTO_1375 Type III effector HopPtoM hopM1 holPtoX hopPtoM Hrp outer protein M1 CEL ORF3 protein protein psesm Pseudomonas syringae pv. tomato (strain DC3000) MISSRIGGAGGVELSRVNQQHDTVPAQTAHPNAVTAGMNPPLTPDQSGSHATESSSAGAARLNVAARHTQLLQAFKAEHGTAPVSGAPMISSRAALLIGSLLQAEPLPFEVMAEKLSPERYQLKQFQGSDLQQRLEKFAQPGQIPDKAEVGQLIKGFAQSVADQLEHFQLMHDASPATVGQHAKADKATLAVSQTALGEYAGRASKAIGEGLSNSIASLDEHISALDLTLQDAEQGNKESLHADRQALVDAKTTLVGLHADFVKSPEAKRLASVAAHTQLDNVVSDLVTARNTVGGWKGAGPIVAAAVPQFLSSMTHLGYVRLSTSDKLRDTIPETSSDANMLKASIIGMVAGIAHETVNSVVKPMFQAALQKTGLNERLNMVPMKAVDTNTVIPDPFELKSEHGELVKKTPEEVAQDKAFVKSERALLNQKKVQGSSTHPVGELMAYSAFGGSQAVRQMLNDVHQINGQTLSARALASGFGGAVSASSQTLLQLKSNYVDPQGRKIPVFTPDRAESDLKKDLLKGMDLREPSVRTTFYSKALSGIQSSALTSALPPVTAQAEGASGTLSAGAILRNMALAATGSVSYLSTLYTNQSVTAEAKALKAAGMGGATPMLDRTETALNNIRHPNRESLPHTFQKSTLSGIPRVAENAYHMGRGALQLPTQMAVDTVRVVDEGVLNAVASAREALKQPTKDDDALRALEEGLLDPR pco3_arath Plant cysteine oxidase 3 PCO3 CpNIFS2 NIFS-like protein 2 F15H18.26 At1g18490 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MLSRLFKAGEKVLSNLVSKKDIYMASRNQEKSPKVQELYDLCKETFTGKAPSPASMAIQKLCSVLDSVSPADVGLEEVSQDDDRGYGVSGVSRFNRVGRWAQPITFLDIHECDTFTMCIFCFPTSSVIPLHDHPEMAVFSKILYGSLHVKAYDWVEPPCIITQDKGVPGSLPARLAKLVSDKVITPQSEIPALYPKTGGNLHCFTALTPCAVLDILSPPYKESVGRSCSYYMDYPFSTFALENGMKKVDEGKEDEYAWLVQIDTPDDLHMRPGSYTGPTIRV f4ihf0_arath T26I20.7 T26I20_7 At2g14910 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATTTLSSFSLSLPQLLHKPTKPLPFLFLLPRFNRRFRSLTITSSSTTSSNNFSSNCGDDGFSLDDFTLHSDSRSPKKCVLSDLIQEIEPLDVSLIQKDVPVTTLDAMKRTISGMLGLLPSDRFQVHIESLWEPLSKLLVSSMMTGYTLRNAEYRLFLEKNLDMSGGGLDSHASENTEYDMEGTFPDEDHVSSKRDSRTQNLSETIDEEGLGRVSSEAQEYILRLQSQLSSVKKELQEMRRKNAALQMQQFVGEEKNDLLDYLRSLQPEKVAELSEPAAPEVKETIHSVVHGLLATLSPKMHSKFPASEVPPTETVKAKSDEDCAELVENTSLQFQPLISLTRDYLARLLFWLEELPSSTSLSLAC q9fjz7_arath K1F13.7 K1F13_7 At5g66420 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKGETYRVFCVGTADTKLDELRFLAGSVRSNIGAFSKNSSSKVEVVIVDVSAGADQKQIDNVADFAFVTREEVLSCYIGSNQEEKPVKLPDDRGEAVGVMSKCLENFLRQAFEDNSLAGAIGLGGSGGTSLISSAFRSLPIGIPKVIVSTVASGQTEPYVGTSDLVLIPSLVDVCGINSVSRVVFSNAGASFAGMVVGRLELFRSSPSDNGKCTVGITMFGVTTPCVNAVQEILTREGYETLVFHATGVGGRAMESLVKEGFIQGVMDITTTEVADHLVGGVMACDSSRFDITIEKGIPLVLSVGALDMVNFGGKDTIPSHFQTRKIHVHNEQVSLIRTTAEENKKFARFIADKLNKSTSKVRVCIPEKGLSALDAPGKPFCDPEATGALINELQGLIQTNDDRQVNIYSHHINDPEFAEALVASFLEICPKTYAQIKPSETTSTKPSTGEHDDGHVRSRLERIPYSPKEFPNAKPETLERTQAILGRLRDQIEKGIPIIGGGAGTGISAKFEEAGGIDLIVIYNSGRFRMAGRGSLAGLLPFADANAVVLEMANEVLPVVKAVPVLAGVCATDPFRRMDYFLKQLESIGFVGVQNFPTVGLFDGNFRQNLEETGMGYGLEVKMISEAHKMGLLTTPYAFNPKEGEEMAKAGADIIVAHMGLTTSGNIGAKTAVSVEESVVRVQAIADAARRFNPDIIVLCHGGPISGPEEAEFVLKRTQGCVHGFYGASSMERLPVEQAITNTVQKYKSISIK o82240_arath F17A22.10 At2g47710 At2g47710/F17A22.10 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATGDGKSVMVVGVDDSEQSTYALEWTLDRFFAPYAPNYPFKLFIVHAKPNAVSAVGLAGPGTAEVVPYVDADLKHTAAKVVEKAKAICQSRSVHGAVIEVFEGDARNILCEVVDKHHASILVVGSHGYGAIKRAVLGSTSDYCAHHAHCSVMIVKKPKIKV big5_arath Brefeldin A-inhibited guanine nucleotide-exchange protein 5 BIG5 ARF guanine-nucleotide exchange factor BIG5 ATMIN7 BEN1 Protein BFA-VISUALIZED ENDOCYTIC TRAFFICKING DEFECTIVE 1 Protein HOPM INTERACTOR 7 F7K15_150 At3g43300 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MAAGGFLTRAFDTMLKESGGKKFPDLQKAIQAYQDGSKVVTQAAPSSIVESSQAEGGGEKTGVEADEPQKVTSAEVAQQASQSKSETINVSLANAGHTLGGAEVELVLKPLRLAFETKNLKIFDAALDCLHKLIAYDHLEGDPGLDGGKNSAPFTDILNMVCSCVDNSSPDSTVLQVLKVLLTAVASGKFKVHGEPLLGVIRVCYNIALNSKSPINQATSKAMLTQMISIVFRRMETDIVSASSTVSQEEHVSGDTSSPKNEEITAADENEKEMTLGDALTQAKDTTLASVEELHTLVGGADIKGLEAALDKAVHLEDGKKIKRGIELESMSIGQRDALLVFRTLCKMGMKEDSDEVTTKTRILSLELLQGMLEGVSHSFTKNFHFIDSVKAYLSYALLRASVSQSSVIFQYASGIFSVLLLRFRDSLKGEIGIFFPIIVLRSLDNSECPNDQKMGVLRMLEKVCKDPQMLVDVYVNYDCDLEAPNLFERMVTTLSKIAQGSQSADPNPAMASQTASVKGSSLQCLVNVLKSLVDWEKIRREAENSTRNANEDSASTGEPIETKSREDVPSNFEKAKAHKSTMEAAISEFNRNSVKGVEYLIANKLVERNPASVAQFLRSTSSLSKVMIGDYLGQHEEFPLAVMHAYVDSMKFSEMKFHSAIREFLKGFRLPGEAQKIDRIMEKFAERYCADNPGLFKNADTAYVLAYAVIMLNTDAHNPMVWPKMSKSDFTRMNATNDPEDCAPTELLEEIYDSIVQEEIKLKDDDTMKKLSSQRPGGEERGGLVSILNLGLPKRISAADAKSETEDIVRKTQEIFRKHGVKRGVFHTVEQVDIIRPMVEAVGWPLLAAFSVTMEVGDNKPRILLCMEGFKAGIHIAYVLGMDTMRYAFLTSLVRFTFLHAPKEMRSKNVEALRILLGLCDSEPDTLQDTWNAVLECVSRLEFIISTPGIAATVMHGSNQISRDGVVQSLKELAGRPAEQVFVNSVKLPSESVVEFFTALCGVSAEELKQSPARVFSLQKLVEISYYNIARIRMVWARIWSVLAEHFVSAGSHHDEKIAMYAIDSLRQLGMKYLERAELTNFTFQNDILKPFVIIMRNTQSQTIRSLIVDCIVQMIKSKVGSIKSGWRSVFMIFTAAADDEVESIVEKSFENVEQVILEHFDQVIGDCFMDCVNCLIRFANNKASDRISLKAIALLRICEDRLAEGLIPGGVLKPVDGNEDETFDVTEHYWFPMLAGLSDLTSDYRPEVRNCALEVLFDLLNERGNKFSTPFWESIFHRILFPIFDHVSHAGKESLISSGDVKFRETSIHSLQLLCNLFNTFYKEVCFMLPPLLSLLLDCAKKSDQTVVSISLGALVHLIEVGGHQFSEGDWDMLLKSIRDASYTTQPLELLNALSFDNPKKNLVLAGDIEADASDSPRVDRNPDDIKDNGKVSAQASPRIGTHGTSLESGIPPKADGSEGRPSSSGRAQKDVDDVNLQRSQTFGQRFMDNLFLRNLTSQPKSSVAEVTVPSSPYKHEDPTEPDSREEESPALGAIRGKCITQLLLLGAINSIQQKYWSNLKTPQKIAIMDILFSFIEFASSYNSYSNLRTRMNHIPTERPPLNLLRQELEGTTIYLDVLQKTTSGLADDASNSEDRLEGAAEEKLVSFCEQVLKETSDLQSTLGETTNMDVHRVLELRSPVIVKVLEGMCFMNNTIFRKHMREFYPLLTRLVCCEQMEIRGALANLFKAQLKPLLQQ q9fmg6_arath MSJ1.2 MSJ1_2 At5g64180 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEQFGGSDERWKGSLENITEMASNLDSLQKLLLKKAVFVEEDTFSRASLVSEQARTIKVLEQRVQTLERELDAAITAAAHARSEKRQAESSQKAAESRAQDVTKELENTTKVFKLHMEELRGMQEQISKRDNEIKLLEAIIQTLGGKERLGKSDVNG 14338_arath 14-3-3-like protein GF14 kappa GRF8 General regulatory factor 8 MNA5.16 At5g65430 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATTLSRDQYVYMAKLAEQAERYEEMVQFMEQLVSGATPAGELTVEERNLLSVAYKNVIGSLRAAWRIVSSIEQKEESRKNEEHVSLVKDYRSKVETELSSICSGILRLLDSHLIPSATASESKVFYLKMKGDYHRYLAEFKSGDERKTAAEDTMIAYKAAQDVAVADLAPTHPIRLGLALNFSVFYYEILNSSEKACSMAKQAFEEAIAELDTLGEESYKDSTLIMQLLRDNLTLWTSDMQEQMDEA hopm1-atmin2 1330489 n/a 1330492 unspecified role unspecified role prey prey n/a 1330493 unspecified role unspecified role bait bait n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S1 hopm1-atmin3 1330489 n/a 1330494 unspecified role unspecified role prey prey n/a 1330493 unspecified role unspecified role bait bait n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S1 hopm1-atmin4 1330489 n/a 1330493 unspecified role unspecified role bait bait n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1330495 unspecified role unspecified role prey prey physical association physical association false false false Table S1 hopm1-atmin6 1330489 n/a 1330496 unspecified role unspecified role prey prey n/a 1330493 unspecified role unspecified role bait bait n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S1 hopm1-atmin7 1330489 n/a 1330497 unspecified role unspecified role prey prey n/a 1330493 unspecified role unspecified role bait bait n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S1 hopm1-atmin9 1330489 n/a 1330498 unspecified role unspecified role prey prey n/a 1330493 unspecified role unspecified role bait bait n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S1 hopm1-atmin10 1330489 n/a 1330493 unspecified role unspecified role bait bait n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1330499 unspecified role unspecified role prey prey physical association physical association false false false Table S1 hopm1-atmin11 1330489 n/a 1330500 unspecified role unspecified role prey prey n/a 1330493 unspecified role unspecified role bait bait n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Table S1 hopm1-atmin2 1330490 n/a 1330493 unspecified role unspecified role bait bait n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1330492 unspecified role unspecified role prey prey physical association physical association false false false Fig. S2A hopm1-atmin7-1 1330490 n/a 1330493 unspecified role unspecified role bait bait n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1330497 unspecified role unspecified role prey prey physical association physical association false false false Fig. S2A hopm1-atmin10-1 1330490 n/a 1330499 unspecified role unspecified role prey prey n/a 1330493 unspecified role unspecified role bait bait n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. S2A hopm1-atmin7-2 1330491 n/a 1330493 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n/a 1330497 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 1330547 1330548 physical association physical association false false false Fig. 2B IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kabisch-2005-1 Type III secretion chaperones ShcS1 and ShcO1 from Pseudomonas syringae pv. tomato DC3000 bind more than one effector. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kabisch U., Landgraf A., Krause J., Bonas U., Boch J. boch@genetik.uni-halle.de Microbiology (Reading, Engl.) (1350-0872) 2005 Only protein-protein interactions imex curation kabisch-2005-2 Type III secretion chaperones ShcS1 and ShcO1 from Pseudomonas syringae pv. tomato DC3000 bind more than one effector. in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kabisch U., Landgraf A., Krause J., Bonas U., Boch J. boch@genetik.uni-halle.de Microbiology (Reading, Engl.) (1350-0872) 2005 Only protein-protein interactions imex curation q88bp9_psesm shcO1 PSPTO_A0017 protein protein psesm Pseudomonas syringae pv. tomato (strain DC3000) MRTSVNGLLEHSLKTLGFDTSALQALRDDGYLLWQGKDKQASLLVPSTDGDALFAICTLSRVDPEHDGRLLALALHLNLSPVHTMSACIALDVEQNTLCLRYTHDLGGNGADTLLLALENAQALAEQIKQVIENFRHDQGRR q87wf2_psesm shcS1 PSPTO_4599 protein protein psesm Pseudomonas syringae pv. tomato (strain DC3000) MNAFATGQLEYSLKKLGYDAAALQALREEGYLLWKGKNDQTSLLVPSADLDALFVINTLSYIDPEHDGRLLALALHLNLSPVHTMSACIALDVEQNTLCLRYTHDLGGSGADTLLLALENAQALAEQVRQVIETFRRDQGRPSGQTSLSRQSSALMR q87wf4_psesm hopS1 PSPTO_4597 protein protein psesm Pseudomonas syringae pv. tomato (strain DC3000) MKISGSTSPAHTSTNSAQKSSSKGLLSGLAKHFKGMLVSGNTSGHSALGHYASSSSGSKGKAPVRDDYSNGPQTRLNNTPLKRALARELDRFGYGSSATESFDRSLQRKDKNPELGKV q87wg2_psesm hopS2 PSPTO_4588 protein protein psesm Pseudomonas syringae pv. tomato (strain DC3000) MKKSGAGTQAYALFASATGSSSKGVLSTIARHLTGCFAPNKTALHSATAVSYELLPGNYSVAASVHGLSVDHRQPALTRLSNVLFNQALALDLERFDEGAPADEMFRPSLKREGAHPRLADSLGGEQLAVQTMEKGLKRLAEDPAQSFARCHSFFYPISSDTTSPQASLHSVASSSG q87wg1_psesm shcS2 PSPTO_4589 protein protein psesm Pseudomonas syringae pv. tomato (strain DC3000) MIAFATGLLEHSLKRLGYDAADLQSLRDEGYLLWHGKNGHTSLLVPAAGGDALFVISTLSYIDPEQDGRLLALALHLNLSPAHTLGASIALDIEQNTLCLRYTHDLTGHGTDNLSRALESTQALAEQIKQVIETFRSEFGRPPMPAHTARRPDAVAL q88bp8_psesm NAD(+)--protein-arginine ADP-ribosyltransferase hopO1-1 PSPTO_A0018 protein protein psesm Pseudomonas syringae pv. tomato (strain DC3000) MGNICGTSGSNHVYSPPISPQHASGSSTPVPSASGTMLSLSHEQILSQNYASNIKGKYRTNPRKGPSPRLSDTLMKQALSSVITQEKKRLKSQPKSIAQDIQPPNSMIKNALDEKDSHPFGDCFSDDEFLAIHLYTSCLYRPINHHLRYAPKNDVAPVVEAMNSGLAKLAQYPDYQVSGQLHRGIKQKMDDGEVMSRFKPGNTYRDDAFMSTSTRMDVTEEFTSDVTLHLQSSSAVNIGPFSKNPYEDEALIPPLTPFKVTGLHKQDDRWHVHLNEIAESSDE shcs1-hops1 773035 n/a 773037 unspecified role unspecified role bait bait n/a 773038 unspecified role unspecified role prey prey physical association physical association false false false 3 shco1-hops1 773035 n/a 773037 unspecified role unspecified role bait bait n/a 773039 unspecified role unspecified role prey prey physical association physical association false false false 3 shco1-hopo1 773035 n/a 773040 unspecified role unspecified role bait bait n/a 773039 unspecified role unspecified role prey prey physical association physical association false false false 3 shcs2-hopo1 773035 n/a 773040 unspecified role unspecified role bait bait n/a 773041 unspecified role unspecified role prey prey physical association physical association false false false 3 shcs1-hopo1 773035 n/a 773038 unspecified role unspecified role prey prey n/a 773040 unspecified role unspecified role bait bait physical association physical association false false false 3 shcs1-hops2 773035 n/a 773038 unspecified role unspecified role prey prey n/a 773042 unspecified role unspecified role bait bait physical association physical association false false false 3 shcs1-hops1-1 773035 n/a 773037 unspecified role unspecified role prey prey n/a 773038 unspecified role unspecified role bait bait physical association physical association false false false 3 shco1-hops1-1 773035 n/a 773039 unspecified role unspecified role bait bait n/a 773037 unspecified role unspecified role prey prey physical association physical association false false false 3 shco1-hopo1-1 773035 n/a 773039 unspecified role unspecified role bait bait n/a 773040 unspecified role unspecified role prey prey physical association physical association false false false 3 shcs1-hopo1-1 773035 n/a 773040 unspecified role unspecified role prey prey n/a 773038 unspecified role unspecified role bait bait physical association physical association false false false 3 shco1-shco1 773035 n/a 773039 unspecified role unspecified role bait bait n/a 773039 unspecified role unspecified role prey prey physical association physical association false false false 6 shco1-shcs1 773035 n/a 773039 unspecified role unspecified role prey prey n/a 773038 unspecified role unspecified role bait bait physical association physical association false false false 6 shco1-shcs1-1 773035 n/a 773039 unspecified role unspecified role bait bait n/a 773038 unspecified role unspecified role prey prey physical association physical association false false false 6 shcs1-shcs1 773035 n/a 773038 unspecified role unspecified role prey prey n/a 773038 unspecified role unspecified role bait bait physical association physical association false false false 6 shcs1-hops1-2 773036 n/a 773037 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) n/a 773038 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4 MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint nicaise-2013-1 Pseudomonas HopU1 modulates plant immune receptor levels by blocking the interaction of their mRNAs with GRP7. nicbe Nicotiana benthamiana anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Nicaise V., Joe A., Jeong B.R., Korneli C., Boutrot F., Westedt I., Staiger D., Alfano J.R., Zipfel C. jalfano2@unl.edu, cyril.zipfel@tsl.ac.uk EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation nicaise-2013-2 Pseudomonas HopU1 modulates plant immune receptor levels by blocking the interaction of their mRNAs with GRP7. nicbe Nicotiana benthamiana bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Nicaise V., Joe A., Jeong B.R., Korneli C., Boutrot F., Westedt I., Staiger D., Alfano J.R., Zipfel C. jalfano2@unl.edu, cyril.zipfel@tsl.ac.uk EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation fls2_arath LRR receptor-like serine/threonine-protein kinase FLS2 FLS2 Protein FLAGELLIN-SENSING 2 Protein FLAGELLIN-SENSITIVE 2 MPL12.13 MPL12.8 At5g46330 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKLLSKTFLILTLTFFFFGIALAKQSFEPEIEALKSFKNGISNDPLGVLSDWTIIGSLRHCNWTGITCDSTGHVVSVSLLEKQLEGVLSPAIANLTYLQVLDLTSNSFTGKIPAEIGKLTELNQLILYLNYFSGSIPSGIWELKNIFYLDLRNNLLSGDVPEEICKTSSLVLIGFDYNNLTGKIPECLGDLVHLQMFVAAGNHLTGSIPVSIGTLANLTDLDLSGNQLTGKIPRDFGNLLNLQSLVLTENLLEGDIPAEIGNCSSLVQLELYDNQLTGKIPAELGNLVQLQALRIYKNKLTSSIPSSLFRLTQLTHLGLSENHLVGPISEEIGFLESLEVLTLHSNNFTGEFPQSITNLRNLTVLTVGFNNISGELPADLGLLTNLRNLSAHDNLLTGPIPSSISNCTGLKLLDLSHNQMTGEIPRGFGRMNLTFISIGRNHFTGEIPDDIFNCSNLETLSVADNNLTGTLKPLIGKLQKLRILQVSYNSLTGPIPREIGNLKDLNILYLHSNGFTGRIPREMSNLTLLQGLRMYSNDLEGPIPEEMFDMKLLSVLDLSNNKFSGQIPALFSKLESLTYLSLQGNKFNGSIPASLKSLSLLNTFDISDNLLTGTIPGELLASLKNMQLYLNFSNNLLTGTIPKELGKLEMVQEIDLSNNLFSGSIPRSLQACKNVFTLDFSQNNLSGHIPDEVFQGMDMIISLNLSRNSFSGEIPQSFGNMTHLVSLDLSSNNLTGEIPESLANLSTLKHLKLASNNLKGHVPESGVFKNINASDLMGNTDLCGSKKPLKPCTIKQKSSHFSKRTRVILIILGSAAALLLVLLLVLILTCCKKKEKKIENSSESSLPDLDSALKLKRFEPKELEQATDSFNSANIIGSSSLSTVYKGQLEDGTVIAVKVLNLKEFSAESDKWFYTEAKTLSQLKHRNLVKILGFAWESGKTKALVLPFMENGNLEDTIHGSAAPIGSLLEKIDLCVHIASGIDYLHSGYGFPIVHCDLKPANILLDSDRVAHVSDFGTARILGFREDGSTTASTSAFEGTIGYLAPEFAYMRKVTTKADVFSFGIIMMELMTKQRPTSLNDEDSQDMTLRQLVEKSIGNGRKGMVRVLDMELGDSIVSLKQEEAIEDFLKLCLFCTSSRPEDRPDMNEILTHLMKLRGKANSFREDRNEDREV q88a91_psesm hopU1 PSPTO_0501 protein protein psesm Pseudomonas syringae pv. tomato (strain DC3000) MNINRQLPVSGSERLLTPDVGVSRQACSERHYSTGQDRHDFYRFAARLHVDAQCFGLSIDDLMDKFSDKHFRAEHPEYRDVYPEECSAIYMHTAQDYSSHLVRGEIGTPLYREVNNYLRLQHENSGREAEIDNHDEKLSPHIKMLSSALNRLMDVAAFRGTVYRGIRGDLDTIARLYHLFDTGGRYVEPAFMSTTRIKDSAQVFEPGTPNNIAFQISLKRGADISGSSQAPSEEEIMLPMMSEFVIEHASALSEGKHLFVLSQI rbg7_arath Glycine-rich RNA-binding protein 7 RBG7 AtRBG7 CCR2 GR-RBP7 GRP7 Glycine-rich protein 7 Protein COLD, CIRCADIAN RHYTHM, AND RNA BINDING 2 F2G1.7 At2g21660 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASGDVEYRCFVGGLAWATDDRALETAFAQYGDVIDSKIINDRETGRSRGFGFVTFKDEKAMKDAIEGMNGQDLDGRSITVNEAQSRGSGGGGGHRGGGGGGYRSGGGGGYSGGGGSYGGGGGRREGGGGYSGGGGGYSSRGGGGGSYGGGRREGGGGYGGGEGGGYGGSGGGGGW efr_arath LRR receptor-like serine/threonine-protein kinase EFR EFR Elongation factor Tu receptor F7C8.70 At5g20480 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MKLSFSLVFNALTLLLQVCIFAQARFSNETDMQALLEFKSQVSENNKREVLASWNHSSPFCNWIGVTCGRRRERVISLNLGGFKLTGVISPSIGNLSFLRLLNLADNSFGSTIPQKVGRLFRLQYLNMSYNLLEGRIPSSLSNCSRLSTVDLSSNHLGHGVPSELGSLSKLAILDLSKNNLTGNFPASLGNLTSLQKLDFAYNQMRGEIPDEVARLTQMVFFQIALNSFSGGFPPALYNISSLESLSLADNSFSGNLRADFGYLLPNLRRLLLGTNQFTGAIPKTLANISSLERFDISSNYLSGSIPLSFGKLRNLWWLGIRNNSLGNNSSSGLEFIGAVANCTQLEYLDVGYNRLGGELPASIANLSTTLTSLFLGQNLISGTIPHDIGNLVSLQELSLETNMLSGELPVSFGKLLNLQVVDLYSNAISGEIPSYFGNMTRLQKLHLNSNSFHGRIPQSLGRCRYLLDLWMDTNRLNGTIPQEILQIPSLAYIDLSNNFLTGHFPEEVGKLELLVGLGASYNKLSGKMPQAIGGCLSMEFLFMQGNSFDGAIPDISRLVSLKNVDFSNNNLSGRIPRYLASLPSLRNLNLSMNKFEGRVPTTGVFRNATAVSVFGNTNICGGVREMQLKPCIVQASPRKRKPLSVRKKVVSGICIGIASLLLIIIVASLCWFMKRKKKNNASDGNPSDSTTLGMFHEKVSYEELHSATSRFSSTNLIGSGNFGNVFKGLLGPENKLVAVKVLNLLKHGATKSFMAECETFKGIRHRNLVKLITVCSSLDSEGNDFRALVYEFMPKGSLDMWLQLEDLERVNDHSRSLTPAEKLNIAIDVASALEYLHVHCHDPVAHCDIKPSNILLDDDLTAHVSDFGLAQLLYKYDRESFLNQFSSAGVRGTIGYAAPEYGMGGQPSIQGDVYSFGILLLEMFSGKKPTDESFAGDYNLHSYTKSILSGCTSSGGSNAIDEGLRLVLQVGIKCSEEYPRDRMRTDEAVRELISIRSKFFSSKTTITESPRDAPQSSPQEWMLNTDMHTM rbg7-efr-1 1448291 n/a GRP7 1448296 anti tag western anti tag western blot anti tag western biological role biological role bait bait over-expressed over expressed level over-expressed living cell living cell tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false nicbe Nicotiana benthamiana n/a EFR 1448297 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false nicbe Nicotiana benthamiana physical association physical association false false false f2a rbg7-efr-2 1448291 n/a GRP7 1448296 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false nicbe Nicotiana benthamiana n/a EFR 1448298 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false nicbe Nicotiana benthamiana physical association physical association false false false f2b hopu1-rbg7 1448291 n/a HopU1 1448299 anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false nicbe Nicotiana benthamiana n/a GRP7 1448296 anti tag western anti tag western blot anti tag western unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false nicbe Nicotiana benthamiana physical association physical association false false false sf6a rbg7-fls2-1 1448292 n/a EFR 1448298 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false nicbe Nicotiana benthamiana n/a GRP7 1448296 tag fluorescence tag visualisation by fluorescence tag fluorescence biological role biological role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false nicbe Nicotiana benthamiana physical association physical association false false false f2e fls2-rbg7 1448292 n/a GRP7 1448296 tag fluorescence tag visualisation by fluorescence tag fluorescence biological role biological role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false nicbe Nicotiana benthamiana n/a EFR 1448297 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false nicbe Nicotiana benthamiana physical association physical association false false false sf3b hopu1-rbg7-2 1448292 n/a GRP7 1448296 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false nicbe Nicotiana benthamiana n/a HopU1 1448299 tag fluorescence tag visualisation by fluorescence tag fluorescence unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false nicbe Nicotiana benthamiana physical association physical association false false false sf6b