mpidb The Microbial Protein Interaction Database chatterjee-2012-1 Structural characterization of a conserved, calcium-dependent periplasmic protease from Legionella pneumophila. ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Chatterjee D., Boyd C.D., O'Toole G.A., Sondermann H. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation boyd-2012-1 LapG, required for modulating biofilm formation by Pseudomonas fluorescens Pf0-1, is a calcium-dependent protease. psefl Pseudomonas fluorescens pull down pull down anti tag western anti tag western blot anti tag western Boyd C.D., Chatterjee D., Sondermann H., O'Toole G.A. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation boyd-2012-2 LapG, required for modulating biofilm formation by Pseudomonas fluorescens Pf0-1, is a calcium-dependent protease. in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Boyd C.D., Chatterjee D., Sondermann H., O'Toole G.A. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation q3kk32_psepf lapF Pfl01_0130 protein protein psepf Pseudomonas fluorescens MFFAGLTFTIKRFLLCPGCLALAVRFAIPRIARWLACALLLAGIMLGGLHADWDFSAISRKATALYGPLGAGQQRIDAWQNLLATQKQVSEMEKLKVVNLFFNKQMRYVEDIDLWHEVDYWETPIEALWKGAGDCEDYAIAKYFSLRHLGVASDKLRITYVKALRQNRAHMVLTYYSSPDAMPLVLDSLIDPIKPAAERTDLLPVYSFNAEGLYLPGAKGNKKVGDTKRLSRWQDVLKKMQAEGFPVETTN q3kk31_psepf lapD Pfl01_0131 protein protein psepf Pseudomonas fluorescens MSLFKQLLIAICLFLVVAFTGSFMVSLESSRTQYVNQLRSHAQDAATALALSLTPNIDDPAMVELLVSSIFDSGYYSSIRVVDLKTDQTIVERNGIPAVTNVPDWFVKLIGLEPAGGDALVSRGWEQAARVEVVSHPMFALAKLWQSALGSLGWLLVCGAVSAVLGALLLRRQLKPLDYMVKQSHAIARREFLSLPDLPRTPELRRVVLAMNQMVEKLKALFQEQAERSEKLRTESYQDNLTGLANRRYFEMQLNARVSNPEQASSGYLLLLRVKDLAGLNQRLGGQRTDELLKAVGEQLSRECAKYPETQNLVTRIRGGEFAVLAPGMTREEALQLAQSLDSALSSLYATGATDVAAVASIGLAPFAHGDSPQAVLSLGDQALAQAEGQGEQNWACLDQSLVADVGDDHHAWHRLLDQALNQRRFELFFQPVVAAQDTQLVLHYKVLSRLLDEQGQTIPAGRFLPWLERFGWTARLDRLMLERVLEQMAGHEESLALNLSSATLADPQALNKVFEILRAHSNLGARLTLEIGEEQLPEQAVLEQLTRRLRELGFSLSLQRFGGRFSMIGNLARLGLAYLKIDGSYIRAIDQESDKRLFIEAIQRAAHSIDLPLIAERVETEGELSVIREMGLYGVQGQLFGEPKPWG q5zxa3_legph lpg0829 protein protein legph Legionella pneumophila subsp. pneumophila MTLTKKMAVGVLIMLLFVFIGTYFITMNNARNFFIQQLESNAQDTATSLGLSLSQSLINHDVPTMDSMVKAVFDRGYFSSIKVQDIKGKVIILKKQLPQESDIPQWFVNLIKWPSTEKSSLIMDGWMQAGVVLVASDPSYVYASLWRNAVEMVNAYLIFALVALVLSYGFLKYLLQPLKRVTAQALAISEHEFPVETKIPKTPELRQVTLAMNQMVTKVKSLFQDQLKQTESLRTQVYQDSLTGMSNRRYFLQHLALILDNEDEFIPGYVMMLVIDGLDELNQKQGYQQGDQLVLTVAKICKSYWKQSSVSTLARINGTTFALISHERDPLVFEKECQEFEQILSQGINDIKICKIHMGAASYFLHQPVSNLLSTVDQAVKKARETGVFYCQKEHDTYKYPQLISGDEIRNSLQQKKISLYAQAVTDGKNCFHKEVFVRIRNQEGEELGAGYFIPVAEKLGLAYPIDQYVLNELTVMDIATHTHFALNISEDTLANKVNSTGYLRQLEDTPAAVLRNLSLEINEAHVLSHFSNSKFFIKQAKKLGVTVGIDRVGIKFSPLHYLSDLNIDYLKLHGSLVTDIDENESKQFFIHYFNEMAKTMDIAVVATQVESEAQWQALQIVHIPWGQGRFLSSVELIK q5zxa4_legph lpg0828 protein protein legph Legionella pneumophila subsp. pneumophila MLKESWLLSKSRPRRLSVIAKRFQKVFLESLRYRLVLSIWTGLILCYVTSFSTQAAPLISVEKIQKLAQSYQGDTRKRFTAWGNLIDSLKKKPVKIQLEKVNSFFNQFNYETDPITGASDDYWKSPVEFIVDGGGDCEDFAIIKYFTLVAVGVPSDQLRITYAASLTLNQAHMVLSFYPTPESEPLILDSLESKILKASARPDLKPVYSFNAEGLWLAKTRGESSLMGDSKSLGKWDALMKRME q3kk29_psepf lapA Pfl01_0133 protein protein psepf Pseudomonas fluorescens MTPGESSMSSVVAIVKSIVGQVFVVSPEGVRRVLVEGDRLFAGDQVDTGVSGAVSLELADGRTIDLGRDTQWSADTPDSSTDLAEATAQAAPSVEELQQAIAAGVDPTTALESTAAGPSAAGTGGAAGGGHSFVMLDATAGRVDPTIGFPTAGINAAGQAAQNITGGQNTDTTANALRESTLSLSATPSITEAGGVLVYTATLTQAPLTDLTITLSNGAVIVIPAGSTTGTANVPLAPNDTVYNDPTQIDVTVTGTTGGSGITVTPPTTPATTQVTDTIDTTTVTLTAGPSVTEGGQITYTATLTNPAQTPVTVTLSNGSTITIGAGQTTGTVNVPTAPNDVYNNGSTVTTTITGATGGNFENLVPNPTPAVTTITDSIDTTGLTLTATGTVVEGGQITYTATLTNPAQTPVTVTLSNGSTITIGAGQTTGSVNVPTAPNDVYNNGGTVSTTITGATGGNFENLVPNTTPATTTVTDSIDTTNLSLSATGSVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKIEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTSTVKLTADSSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTVTTTAPNDALAGNAPLTNSITGVSGGNYENLVADKTPVSTTVTDVADITNLTLSATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDASGGNFENLVPSTVPAVTEVTDTIDTSTVKLSADTSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTITTTAPNDVLTGHAPLTNSITGVSGGNYENLVADKTPVSTTVTDVADTTNLTLSATGTVAEGGSIVYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTNVTDTIDTTTVKLTATESAVEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTITTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSASDSVAEGGSIVYTATLTNPAGTSVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDASGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTRVTDTVDTTNLTLSATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTNVTDTIDTSTVKLSADTSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTITTTAPNDALNGHTPLSNSITGVTGGNYENLVADKTPVSTTVTDTVDTTDLTLTATDSVAEGGSITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTITTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSASDSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEATISTATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAVEGGTVSYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDVLNGHAPLTNAITNVTGGNYENLVADKTPVSTTVTDTVDTTNLTLSATDSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEVTIKDATGGNFENLVPSTAPAVTEVTDTIDTSTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNSITDVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSATPEVNEGGQITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVETTISTTTGGNFENLVTSTAPAVTTVNDTIDTSTVSLTATANVAEGETVVYTATVTAPVTGSPVVVTLSNGQTITIAVGETTGTVNFVAPNSPLAGGSSLSVTIDKATGGNYENLAVDGKSADTSVSDTVDTTNLNLTATDSVAEGGSITYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEATISTATGGGFENLVPSTVPAVTNVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNSITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSATGSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTIGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTASETAAEGGTVTYTATVGAPVTGSPVTVTLANGQQITIEVGKTTGTVTTTAPNDVLNDHAPLTNAITDVSGGNYENLVADKTPVSTTITDTIDTTNLSLTATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGTVEATIKSATGGNFENLVTSTTPAVTNVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLSNGQSITIEVGKTTGTVTTTAPNDALTGHAPLTNAITGVTGGNYENLVADKTPVSTTVTDTVDTTNLTLSATNSVAEGGSIIYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVPSTVPAVTQVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVNLSNGQSITIEVGKTTGTVTTTAPNDVLNGHAPLTNAITGVTGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGTPVTVTLSNGAVIIIEAGKTTGTVTVAAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGTVTYTATVGAPVTGSPVTVNLSNGQSITIEVGKTTGTVTTTAPNDVLTGHAPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGNVVYTATVGAPVTGSPVTVTLSNGQSITIEVGKTSGTVTTTAPNDVLNGHAPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATNSVAEGGSITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGTVTYTATVGAPVTGSPVIVNLANGQSITIEVGKTTGTVTTIAPNDALTGQAPLTNSITNVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATIKSATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATTTAAEGGTVTYTATVGAPVTGSPVTVNLANGQSITIEVGKTTGTVTTTAPNDALTGHTPLTNSITNVTGGNYENLVADKTPVSTTVTDTIDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIDAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVSITGSTSVTEGQTASYTVSLTHPAQTEVTLKIVYSGTAADGSDFTGVYTVKIPAGASSAQFNVATIDDKITEGTENFVVKIDSATGGNFENLAVSSTNGSVSTSIIDNDAPPVIDLDANNSSGATGADYKVTFTEGTTGPGVSIADTDIKITDPDSTMLTGATIVLTNRQDGDALNLGNSVNGITINANSTNGTVTLTLSGNATLADYMQAIKNISFTNSSEDPSTVPRIITVTVTDGGNYSNTATTTVNVVAINDAPVAAPVNVTGTEDTPLILGWSTFGVSDVDSPASSLGVKITQLPGEGKLQYLDGSTWKDVANNQTFSKADIDAGKLRFVPDANESGVNGYGGTGVGNNQADYAQIKFQPTDGQLLGNTGTIKIDITPVADAPTLSVADNSVKSTGLIKEVWTGLSGLGTDGSGANSTTLKNVIDGAGKPNTSGTVNNVQSDGSVTAGTASKTSGLIYLEAGKTYTFSGTGDDSLLVTIGGKSVASTTWGAGGNLNGSFTPTTSGYYTLDIYHHNQSGPGSYDVNLSVNGSTPIDLSSAGVPIYTGVQDLVNAGVTVSDLHGSNGEGYYDGYKLNTGAEGTTVKLSSISTALTDTDGSETLSIKISGAPVGSVLSDGAGHSFTVTATSGDANVTGWNLGTLTVTPPTYYNGQFNLTVTSTSTEQVGGSASTTATIPVTVVPAVYNSIVATSADDTVTGTDGNDIMVADIGGLTVVPGTNYNIAFMVDSSGSMSASSINAAKDSLTSVFNTLKQSLGGSNSGTVNIFLVDFDTQVNKSVSVNLNDPNALTLLKAVLDSMASGGGTNYEDVFKATANFFQSAEAVANTGAKNLTYFITDGQPTYYQSNEQTNPTLYGNVKLDDVVKTSNYKLGSSFATYIDSTHYLSISTSGEVVLQTLKNNNWSWNSLGTIHAQGDGTYEISSLAGSGNSTSSNTTDNSAAGFALLSGLSNVEAIGLNNGVSLNDLKPYDSDKTPQTNIDPKDLANSIIGHTEATMPGNDTVSGGDGNDILFGDLVSFNGIAGEGYQAMQAFVAKETGVDVSKVTTSNVHQYITEHYQAFDVSGAHDGNDTLLGGAGNDILFGSGGNDLLDGGKGNDILLGGTGNDTLIGGQGNDILIGGSGADTFVWKAGDTGNDVIKDFKASEGDRIDLRDLLQGETGSTIDNFLKITTVDGVSSLQVSSGGKFNSGDAAAATPDVTIKLEGNNWSSANIHNLIAGSDPTIKVDHNNS lapf-lapd 1339867 not-defined-88924066 1339869 unspecified role unspecified role bait bait q3kk32-- Q3KK32-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-944179849 1339870 unspecified role unspecified role prey prey Q3KK31:p.Trp125Glu Q3KK31-125-125 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 2 lapf-q5zxa3 1339867 not-defined-776411543 1339869 unspecified role unspecified role bait bait q3kk32-- Q3KK32-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-2072476750 1339871 unspecified role unspecified role prey prey Q5ZXA3:p.Trp126Glu Q5ZXA3-126-126 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 2 q5zxa4-lapd 1339867 not-defined-1412014193 1339870 unspecified role unspecified role prey prey Q3KK31:p.Trp125Glu Q3KK31-125-125 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false not-defined-624403667 1339872 unspecified role unspecified role bait bait q5zxa4-- Q5ZXA4-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 2 q5zxa4-q5zxa3 1339867 not-defined-915833545 1339872 unspecified role unspecified role bait bait q5zxa4-- Q5ZXA4-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false not-defined-1496891226 1339871 unspecified role unspecified role prey prey Q5ZXA3:p.Trp126Glu Q5ZXA3-126-126 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 2 lapd-lapf 1339947 not-defined-2024053639 1339869 unspecified role unspecified role prey prey Q3KK32:p.Glu136Ala Q3KK32-136-136 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false q3kk32-- Q3KK32-- ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q3KK32:p.Asp134Ala Q3KK32-134-134 mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false not-defined-1933455263 1339870 unspecified role unspecified role bait bait q3kk31-- Q3KK31-- his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false figure 5 lapa-lapf 1339948 n/a 1339951 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n-terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain undetermined undetermined sequence position undetermined false not-defined-2024053639 1339869 enzyme enzyme unspecified role unspecified role Q3KK32:p.Asp134Ala Q3KK32-134-134 mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false q3kk32-- Q3KK32-- ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q3KK32:p.Glu136Ala Q3KK32-136-136 mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain false protein cleavage protein cleavage false false false figure 3 DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ newell-2011-1 in vitro In vitro protease assay protease assay anti tag western anti tag western blot anti tag western Newell PD., Boyd CD., Sondermann H., O'Toole GA. DIP original experiment: DIP-216748X PLoS biology 2011 IMEx A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. Newell PD., Boyd CD., Sondermann H., O'Toole GA. Only protein-protein interactions imex curation newell-2011-2 psepf Pseudomonas fluorescens pull down pull down predetermined predetermined participant predetermined Newell PD., Boyd CD., Sondermann H., O'Toole GA. DIP original experiment: DIP-216750X PLoS biology 2011 IMEx A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. Newell PD., Boyd CD., Sondermann H., O'Toole GA. Only protein-protein interactions imex curation newell-2011-3 psepf Pseudomonas fluorescens anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Newell PD., Boyd CD., Sondermann H., O'Toole GA. DIP original experiment: DIP-216749X PLoS biology 2011 IMEx A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. Newell PD., Boyd CD., Sondermann H., O'Toole GA. Only protein-protein interactions imex curation newell-2011-4 psepf Pseudomonas fluorescens pull down pull down predetermined predetermined participant predetermined Newell PD., Boyd CD., Sondermann H., O'Toole GA. DIP original experiment: DIP-216751X PLoS biology 2011 IMEx A c-di-GMP effector system controls cell adhesion by inside-out signaling and surface protein cleavage. Newell PD., Boyd CD., Sondermann H., O'Toole GA. Only protein-protein interactions imex curation navarro-2011-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Navarro MV., Newell PD., Krasteva PV., Chatterjee D., Madden DR., O'Toole GA., Sondermann H. DIP original experiment: DIP-216757X PLoS biology 2011 IMEx Structural basis for c-di-GMP-mediated inside-out signaling controlling periplasmic proteolysis. Navarro MV., Newell PD., Krasteva PV., Chatterjee D., Madden DR., O'Toole GA., Sondermann H. Only protein-protein interactions imex curation navarro-2011-2 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Navarro MV., Newell PD., Krasteva PV., Chatterjee D., Madden DR., O'Toole GA., Sondermann H. DIP original experiment: DIP-216752X PLoS biology 2011 IMEx Structural basis for c-di-GMP-mediated inside-out signaling controlling periplasmic proteolysis. Navarro MV., Newell PD., Krasteva PV., Chatterjee D., Madden DR., O'Toole GA., Sondermann H. Only protein-protein interactions imex curation navarro-2011-3 in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Navarro MV., Newell PD., Krasteva PV., Chatterjee D., Madden DR., O'Toole GA., Sondermann H. DIP original experiment: DIP-216755X PLoS biology 2011 IMEx Structural basis for c-di-GMP-mediated inside-out signaling controlling periplasmic proteolysis. Navarro MV., Newell PD., Krasteva PV., Chatterjee D., Madden DR., O'Toole GA., Sondermann H. Only protein-protein interactions imex curation navarro-2011-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Navarro MV., Newell PD., Krasteva PV., Chatterjee D., Madden DR., O'Toole GA., Sondermann H. DIP original experiment: DIP-216753X PLoS biology 2011 IMEx Structural basis for c-di-GMP-mediated inside-out signaling controlling periplasmic proteolysis. Navarro MV., Newell PD., Krasteva PV., Chatterjee D., Madden DR., O'Toole GA., Sondermann H. Only protein-protein interactions imex curation navarro-2011-5 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Navarro MV., Newell PD., Krasteva PV., Chatterjee D., Madden DR., O'Toole GA., Sondermann H. DIP original experiment: DIP-216756X PLoS biology 2011 IMEx Structural basis for c-di-GMP-mediated inside-out signaling controlling periplasmic proteolysis. Navarro MV., Newell PD., Krasteva PV., Chatterjee D., Madden DR., O'Toole GA., Sondermann H. Only protein-protein interactions imex curation navarro-2011-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Navarro MV., Newell PD., Krasteva PV., Chatterjee D., Madden DR., O'Toole GA., Sondermann H. DIP original experiment: DIP-216754X PLoS biology 2011 IMEx Structural basis for c-di-GMP-mediated inside-out signaling controlling periplasmic proteolysis. Navarro MV., Newell PD., Krasteva PV., Chatterjee D., Madden DR., O'Toole GA., Sondermann H. Only protein-protein interactions imex curation q3kk31_psepf lapD Pfl01_0131 protein protein psepf Pseudomonas fluorescens MSLFKQLLIAICLFLVVAFTGSFMVSLESSRTQYVNQLRSHAQDAATALALSLTPNIDDPAMVELLVSSIFDSGYYSSIRVVDLKTDQTIVERNGIPAVTNVPDWFVKLIGLEPAGGDALVSRGWEQAARVEVVSHPMFALAKLWQSALGSLGWLLVCGAVSAVLGALLLRRQLKPLDYMVKQSHAIARREFLSLPDLPRTPELRRVVLAMNQMVEKLKALFQEQAERSEKLRTESYQDNLTGLANRRYFEMQLNARVSNPEQASSGYLLLLRVKDLAGLNQRLGGQRTDELLKAVGEQLSRECAKYPETQNLVTRIRGGEFAVLAPGMTREEALQLAQSLDSALSSLYATGATDVAAVASIGLAPFAHGDSPQAVLSLGDQALAQAEGQGEQNWACLDQSLVADVGDDHHAWHRLLDQALNQRRFELFFQPVVAAQDTQLVLHYKVLSRLLDEQGQTIPAGRFLPWLERFGWTARLDRLMLERVLEQMAGHEESLALNLSSATLADPQALNKVFEILRAHSNLGARLTLEIGEEQLPEQAVLEQLTRRLRELGFSLSLQRFGGRFSMIGNLARLGLAYLKIDGSYIRAIDQESDKRLFIEAIQRAAHSIDLPLIAERVETEGELSVIREMGLYGVQGQLFGEPKPWG q3kk32_psepf lapF Pfl01_0130 protein protein psepf Pseudomonas fluorescens MFFAGLTFTIKRFLLCPGCLALAVRFAIPRIARWLACALLLAGIMLGGLHADWDFSAISRKATALYGPLGAGQQRIDAWQNLLATQKQVSEMEKLKVVNLFFNKQMRYVEDIDLWHEVDYWETPIEALWKGAGDCEDYAIAKYFSLRHLGVASDKLRITYVKALRQNRAHMVLTYYSSPDAMPLVLDSLIDPIKPAAERTDLLPVYSFNAEGLYLPGAKGNKKVGDTKRLSRWQDVLKKMQAEGFPVETTN q3kk29_psepf lapA Pfl01_0133 protein protein psepf Pseudomonas fluorescens MTPGESSMSSVVAIVKSIVGQVFVVSPEGVRRVLVEGDRLFAGDQVDTGVSGAVSLELADGRTIDLGRDTQWSADTPDSSTDLAEATAQAAPSVEELQQAIAAGVDPTTALESTAAGPSAAGTGGAAGGGHSFVMLDATAGRVDPTIGFPTAGINAAGQAAQNITGGQNTDTTANALRESTLSLSATPSITEAGGVLVYTATLTQAPLTDLTITLSNGAVIVIPAGSTTGTANVPLAPNDTVYNDPTQIDVTVTGTTGGSGITVTPPTTPATTQVTDTIDTTTVTLTAGPSVTEGGQITYTATLTNPAQTPVTVTLSNGSTITIGAGQTTGTVNVPTAPNDVYNNGSTVTTTITGATGGNFENLVPNPTPAVTTITDSIDTTGLTLTATGTVVEGGQITYTATLTNPAQTPVTVTLSNGSTITIGAGQTTGSVNVPTAPNDVYNNGGTVSTTITGATGGNFENLVPNTTPATTTVTDSIDTTNLSLSATGSVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKIEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTSTVKLTADSSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTVTTTAPNDALAGNAPLTNSITGVSGGNYENLVADKTPVSTTVTDVADITNLTLSATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDASGGNFENLVPSTVPAVTEVTDTIDTSTVKLSADTSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTITTTAPNDVLTGHAPLTNSITGVSGGNYENLVADKTPVSTTVTDVADTTNLTLSATGTVAEGGSIVYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTNVTDTIDTTTVKLTATESAVEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTITTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSASDSVAEGGSIVYTATLTNPAGTSVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDASGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTRVTDTVDTTNLTLSATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTNVTDTIDTSTVKLSADTSVAEGGTVTYTATVGAPVTGSPVTVTLANGQTITIEVGKTTGTITTTAPNDALNGHTPLSNSITGVTGGNYENLVADKTPVSTTVTDTVDTTDLTLTATDSVAEGGSITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTITTTAPNDALNGHTPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSASDSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEATISTATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTATESAVEGGTVSYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDVLNGHAPLTNAITNVTGGNYENLVADKTPVSTTVTDTVDTTNLTLSATDSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEVTIKDATGGNFENLVPSTAPAVTEVTDTIDTSTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNSITDVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSATPEVNEGGQITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVETTISTTTGGNFENLVTSTAPAVTTVNDTIDTSTVSLTATANVAEGETVVYTATVTAPVTGSPVVVTLSNGQTITIAVGETTGTVNFVAPNSPLAGGSSLSVTIDKATGGNYENLAVDGKSADTSVSDTVDTTNLNLTATDSVAEGGSITYTATLTNPAGTPVTVTLSNGAVITIEAGKTTGTVTVDAPKDDVYKDAGKVEATISTATGGGFENLVPSTVPAVTNVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLANGQSITIEVGKTTGTVTTTAPNDALNGHTPLTNSITGVSGGNYENLVADKTPVSTTVTDTVDTTNLTLSATGSVAEGGSIVYTATLTNPAGTPVTVTLSNGAVITIEAGKTIGTVTVPAPADDVYKDAGKVEVTIKDATGGNFENLVPSTVPAVTEVTDTIDTTTVKLTASETAAEGGTVTYTATVGAPVTGSPVTVTLANGQQITIEVGKTTGTVTTTAPNDVLNDHAPLTNAITDVSGGNYENLVADKTPVSTTITDTIDTTNLSLTATGTVAEGGSIVYTATLTNPAGTPVTVTLSNGSVITIEAGKTTGTVTVPAPADDVYKDAGTVEATIKSATGGNFENLVTSTTPAVTNVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVTLSNGQSITIEVGKTTGTVTTTAPNDALTGHAPLTNAITGVTGGNYENLVADKTPVSTTVTDTVDTTNLTLSATNSVAEGGSIIYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVPSTVPAVTQVTDTIDTTTVKLTATESAAEGGTVTYTATVGAPVTGSPVTVNLSNGQSITIEVGKTTGTVTTTAPNDVLNGHAPLTNAITGVTGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGTPVTVTLSNGAVIIIEAGKTTGTVTVAAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGTVTYTATVGAPVTGSPVTVNLSNGQSITIEVGKTTGTVTTTAPNDVLTGHAPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGNVVYTATVGAPVTGSPVTVTLSNGQSITIEVGKTSGTVTTTAPNDVLNGHAPLTNAITGVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATNSVAEGGSITYTATLTNAAGTPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATATAAEGGTVTYTATVGAPVTGSPVIVNLANGQSITIEVGKTTGTVTTIAPNDALTGQAPLTNSITNVSGGNYENLVADKTPVSTTVTDTVDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIEAGKTTGTVTVPAPADDVYKDAGNVQATIKSATGGNFENLVTSTTPAVTSVTDTIDTTTVKLTATTTAAEGGTVTYTATVGAPVTGSPVTVNLANGQSITIEVGKTTGTVTTTAPNDALTGHTPLTNSITNVTGGNYENLVADKTPVSTTVTDTIDTTNLSLSATGTVAEGGSITYTATLTNAAGSPVTVTLSNGAVITIDAGKTTGTVTVPAPADDVYKDAGNVQATITNATGGNFENLVTSTTPAVTSVTDTIDTTTVSITGSTSVTEGQTASYTVSLTHPAQTEVTLKIVYSGTAADGSDFTGVYTVKIPAGASSAQFNVATIDDKITEGTENFVVKIDSATGGNFENLAVSSTNGSVSTSIIDNDAPPVIDLDANNSSGATGADYKVTFTEGTTGPGVSIADTDIKITDPDSTMLTGATIVLTNRQDGDALNLGNSVNGITINANSTNGTVTLTLSGNATLADYMQAIKNISFTNSSEDPSTVPRIITVTVTDGGNYSNTATTTVNVVAINDAPVAAPVNVTGTEDTPLILGWSTFGVSDVDSPASSLGVKITQLPGEGKLQYLDGSTWKDVANNQTFSKADIDAGKLRFVPDANESGVNGYGGTGVGNNQADYAQIKFQPTDGQLLGNTGTIKIDITPVADAPTLSVADNSVKSTGLIKEVWTGLSGLGTDGSGANSTTLKNVIDGAGKPNTSGTVNNVQSDGSVTAGTASKTSGLIYLEAGKTYTFSGTGDDSLLVTIGGKSVASTTWGAGGNLNGSFTPTTSGYYTLDIYHHNQSGPGSYDVNLSVNGSTPIDLSSAGVPIYTGVQDLVNAGVTVSDLHGSNGEGYYDGYKLNTGAEGTTVKLSSISTALTDTDGSETLSIKISGAPVGSVLSDGAGHSFTVTATSGDANVTGWNLGTLTVTPPTYYNGQFNLTVTSTSTEQVGGSASTTATIPVTVVPAVYNSIVATSADDTVTGTDGNDIMVADIGGLTVVPGTNYNIAFMVDSSGSMSASSINAAKDSLTSVFNTLKQSLGGSNSGTVNIFLVDFDTQVNKSVSVNLNDPNALTLLKAVLDSMASGGGTNYEDVFKATANFFQSAEAVANTGAKNLTYFITDGQPTYYQSNEQTNPTLYGNVKLDDVVKTSNYKLGSSFATYIDSTHYLSISTSGEVVLQTLKNNNWSWNSLGTIHAQGDGTYEISSLAGSGNSTSSNTTDNSAAGFALLSGLSNVEAIGLNNGVSLNDLKPYDSDKTPQTNIDPKDLANSIIGHTEATMPGNDTVSGGDGNDILFGDLVSFNGIAGEGYQAMQAFVAKETGVDVSKVTTSNVHQYITEHYQAFDVSGAHDGNDTLLGGAGNDILFGSGGNDLLDGGKGNDILLGGTGNDTLIGGQGNDILIGGSGADTFVWKAGDTGNDVIKDFKASEGDRIDLRDLLQGETGSTIDNFLKITTVDGVSSLQVSSGGKFNSGDAAAATPDVTIKLEGNNWSSANIHNLIAGSDPTIKVDHNNS lapa-lapf-1 3222506 N/A 3222510 enzyme target enzyme target substrate substrate unspecified role unspecified role N-terminal domain Region 8-242 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3222511 enzyme enzyme unspecified role unspecified role Q3KK32:p.Cys135Ala C135A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli protein cleavage protein cleavage false false false lapd-lapf-1 3222507 N/A 3222512 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false psepf Pseudomonas fluorescens N/A 3222511 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false psepf Pseudomonas fluorescens physical association physical association false false false lapf-lapd-1 3222508 N/A 3222512 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false psepf Pseudomonas fluorescens N/A 3222511 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false psepf Pseudomonas fluorescens physical association physical association false false false lapd-lapf-2 3222509 N/A 3222512 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q3KK31:p.Glu617Ala E617A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q3KK31:p.Leu152Pro L152P mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false psepf Pseudomonas fluorescens N/A 3222511 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false psepf Pseudomonas fluorescens physical association physical association false false false lapd-1 3222536 N/A 3222512 unspecified role unspecified role unspecified role unspecified role Output domain Region 22-151 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lapf-lapd-2 3222537 N/A 3222512 unspecified role unspecified role prey prey Output domain Region 22-151 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false Q3KK31:p.Trp125Glu W125E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3222511 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli direct interaction direct interaction false false false lapd-3 3222538 N/A 3222512 unspecified role unspecified role unspecified role unspecified role EAL domain Region 399-648 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q3KK31:p.Ala602Glu A602E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q3KK31:p.[Ser229Asp;Ala602Glu] S229D/A602E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lapd-4 3222539 N/A 3222512 unspecified role unspecified role unspecified role unspecified role EAL domain Region 399-648 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lapd-5 3222540 N/A 3222512 unspecified role unspecified role unspecified role unspecified role Dual domain Region 220-648 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lapd-6 3222541 N/A 3222512 unspecified role unspecified role unspecified role unspecified role EAL domain Region 399-648 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q3KK31:p.Ala602Glu A602E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Q3KK31:p.[Ser229Asp;Ala602Glu] S229D/A602E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false