DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kashiwagi-2016-3 picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Kashiwagi K., Takahashi M., Nishimoto M., Hiyama TB., Higo T., Umehara T., Sakamoto K., Ito T., Yokoyama S. DIP original experiment: DIP-268711X Nature 2016 IMEx Crystal structure of eukaryotic translation initiation factor 2B. Kashiwagi K., Takahashi M., Nishimoto M., Hiyama TB., Higo T., Umehara T., Sakamoto K., Ito T., Yokoyama S. Only protein-protein interactions imex curation kashiwagi-2016-4 in vitro In vitro crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Kashiwagi K., Takahashi M., Nishimoto M., Hiyama TB., Higo T., Umehara T., Sakamoto K., Ito T., Yokoyama S. DIP original experiment: DIP-268710X Nature 2016 IMEx Crystal structure of eukaryotic translation initiation factor 2B. Kashiwagi K., Takahashi M., Nishimoto M., Hiyama TB., Higo T., Umehara T., Sakamoto K., Ito T., Yokoyama S. Only protein-protein interactions imex curation kashiwagi-2016-6 in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Kashiwagi K., Takahashi M., Nishimoto M., Hiyama TB., Higo T., Umehara T., Sakamoto K., Ito T., Yokoyama S. DIP original experiment: DIP-268712X Nature 2016 IMEx Crystal structure of eukaryotic translation initiation factor 2B. Kashiwagi K., Takahashi M., Nishimoto M., Hiyama TB., Higo T., Umehara T., Sakamoto K., Ito T., Yokoyama S. Only protein-protein interactions imex curation kashiwagi-2016-7 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Kashiwagi K., Takahashi M., Nishimoto M., Hiyama TB., Higo T., Umehara T., Sakamoto K., Ito T., Yokoyama S. DIP original experiment: DIP-268708X Nature 2016 IMEx Crystal structure of eukaryotic translation initiation factor 2B. Kashiwagi K., Takahashi M., Nishimoto M., Hiyama TB., Higo T., Umehara T., Sakamoto K., Ito T., Yokoyama S. Only protein-protein interactions imex curation ehara-2019-1 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Ehara H., Kujirai T., Fujino Y., Shirouzu M., Kurumizaka H., Sekine SI. shunichi.sekine@riken.jp (S.S.); kurumizaka@iam.u-tokyo.ac.jp Science (New York, N.Y.) 2019 imex curation Only protein-protein interactions imex curation ehara-2017-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ehara H., Yokoyama T., Shigematsu H., Yokoyama S., Shirouzu M., Sekine SI., Ehara H., Yokoyama T., Shigematsu H., Yokoyama S., Shirouzu M., Sekine SI. shunichi.sekine@riken.jp Science (New York, N.Y.) 2017 imex curation Only protein-protein interactions imex curation ehara-2017-2 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Ehara H., Yokoyama T., Shigematsu H., Yokoyama S., Shirouzu M., Sekine SI., Ehara H., Yokoyama T., Shigematsu H., Yokoyama S., Shirouzu M., Sekine SI. shunichi.sekine@riken.jp Science (New York, N.Y.) 2017 imex curation Only protein-protein interactions imex curation kujirai-2018-1 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog experimental particp experimental participant identification experimental particp Kujirai T., Ehara H., Fujino Y., Shirouzu M., Sekine SI., Kurumizaka H. kurumizaka@iam.u-tokyo.ac.jp Science (New York, N.Y.) 2018 imex curation Only protein-protein interactions imex curation c4r4y0_kompg DNA-directed RNA polymerase subunit PAS_chr3_0568 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MSQFPYSSAPLRSVKEVQFGLLSPEEIRAISVVKIEYPEIMDESRQRPREGGLNDPKLGSIDRNFKCQTCGEGMAECPGHFGHMELAKPVFHIGFIPKIKKVCECICMNCGKLLLDETNPTMAQAIRIRDPKKRFNAVWQLCKTKMVCEADAPVDEYSEQKVVSRGGCGNTQPVVRKDGMKLWGTWKKSGFSDRDAQPERKLLTPGEILNVFKHISPEDCFRLGFNEDYARPEWMIITVLPVPPPQVRPSIAMDETTQGQDDLTHKLSDILKANINVQKLEMDGSPQHIINEVEQLLQFHVATYMDNDIAGQPQALQKSGRPVKAIRARLKGKEGRLRGNLMGKRVDFSARTVISGDPNLELDQVGVPISIAKTLSYPETVTQYNIHRLTEYVRNGPNEHPGAKYVIRDNGDRIDLRYHKRAGDIVLQYGWKVERHLMDDDPVLFNRQPSLHKMSMMAHRVKVMPYSTFRLNLSVTSPYNADFDGDEMNLHVPQSEETRAELSQLCAVPLQIVSPQSNKPVMGIVQDTLCGVRKMTLRDTFIEYEQVMNMLFWVPSWDGVVPQPAILKPKPLWTGKQLLSIAIPSGIHLQRTDGGNSLLSPKDNGMLIVDGKVMFGVVDKKTVGSGGGGLIHTVMREKGPKICAELFGNIQKVVNYWLLHNGFSIGIGDAIADASTMKEITHAISSAKEQVQEIIYKAQHNELELKPGMTLRESFEGEVSRTLNDARDSAGRSAEMNLKDLNNVKQMVSAGSKGSFINIAQMSACVGQQMVEGKRIAFGFADRSLPHFTKDDFSPESKGFVENSYLRGLTPQEFFFHAMAGREGLIDTAVKTAETGYIQRRLVKALEDIMVHYDGTTRNSLGDIIQFLYGEDGLDGTQVERQTIDTIPGSDKAFHKRYYVDLMDEKNSIKPDVIEYAADILGDVELQKELNSEYEQLVSDRKFLREIVFVNGDHNWPLPVNLRRIIQNAQQIFHLDRAKASDLTIPEIIHGVRDLCKKLFVLRGENELIKEAQQNATSLFQCLVRARLATRRILEEFRLNRDAFEWVLGTIEAQFQRSLVHPGEMVGVIAAQSIGEPATQMTLNTFHYAGVSSKNVTLGVPRLKEILNVAKNIKTPALTVYLDREIALDIEKAKVIQSSIEYTTLKNVTSATEIYYDPDPTSTVIEEDFDTVEAYFSIPDEKVEETIDKQSPWLLRLELDRARMLDKQLTMNQVADKISEVFSDDLFVMWSEDNADKLIIRCRVIRDPKAMDEELEAEEDQMLKRIEAHMLDLIALRGIPGISKVYMVKHKVSVPDESGEYKNEELWALETDGINLAEVMAVPGVDSSRTYSNSFVEILSVLGIEATRSSLYKEILNVIAFDGSYVNYRHMALLVDVMTSRGYLMAITRHGINRADTGALMRCSFEETVEILFEAGAAAELDDCRGVSENVMLGQLAPMGTGAFDVMIDEKLLTSLPADYAPTMPLFKGKATQGSATPYDNNAQYDDEFNHDDVADVMFSPMAETGSGDDRSGGLTEYAGIQSPYQPTSPGLSATSPGFAPTSPGFAPTSPRYSPTSPGYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPSYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPTSPQYSPASPQYSPSRHSPNGESKEGE c4r7l2_kompg PAS_chr4_0344 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MSKEPKVNIINAQDDEVELMLSDVNLSLANSLRRTMLAEVPTLAIDLVEIKMNTSVLADEFISHRLGLIPLVSEDVEEMKYSRDCTCEDYCDECSVVLELSARHEGEEGTTDVYSSSLIKVSGPGNLNVGEPVRRDDYDQGILLCKLRNHQELNIRCIAKKGIAKEHAKWSPCSAIAFEYDPHNKLKHTDFWFEVDAKKEWPDSKYATWEEPPKPGEVFDYKAKPNRFYMTVETTGSLKANQVFSRGIKTLQEKLANVLFELENSRPANTTAYGGATAYGGQTVYGRETSYGGNTNYGDYNAPY c4qzq7_kompg DNA-directed RNA polymerase subunit beta PAS_chr2-1_0125 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MSYDPYSIDDTITTEDCWTVISAFFEEKGLVSQQLDSFDEFMETSIQDLVWEEPRLILDQPAQHTNEKDNINKRYEIRFGKIYLSRPTMTEADGTTHAMFPQEARLRNLTYSSPVYLDMEKSMFTSIDDEGNPNATLDWQQVHEPIKDGVEEGNKVHIGKVPIMLRSKFCSLRTLDEVDLYKMKECPYDMGGYFVINGSEKVLIAQERSAANIVQVFKKAAPSPISHVAEIRSALEKGSRLISTMQIKLYGREDKGTGRTIKATLPYVKQDIPIVIVFRALGVVPDGEILQHICYDENDWQMLEMLKPCIEEGFVIQDKEVALDFIGRRGSAALGIRREKRIQYAKDILQKELLPHITQEEGFETRKTFFLGYMVNRLLLCALERKDQDDRDHFGKKRLDLAGPLLANLFRILFRKLTREIYRYMQRCIETDRDFNLNLAVKSTTITSGLKYSLATGNWGEQKKAMSSRAGVSQVLNRYTYSSTLSHLRRTNTPIGRDGKLAKPRQLHNTHWGLVCPAETPEGQACGLVKNLSLLSGISIGSPSEPIINFLEEWGMEPLEDYDPAQHTKSTRIFVNGVWTGIHRDPSMLVSTMRDLRRSGAISPEVSIIRDIREREFKIFTDVGRVYRPLFIVEDDESKDNKGELRITKEHIRKIQQGYDDDAMNDDSEEQEQDVYGWSSLVTSGVIEYVDGEEEETIMIAMTPEDLQTRSLEQKEIDLNDTAKRIKPEMSTSSHHTFTHCEIHPSMILGVAASIIPFPDHNQSPRNTYQSAMGKQAMGVFLTNYNVRMDTMANILYYPQKPLAKTQAMEYLKFRELPAGQNAIVAIACYSGYNQEDSMIMNQSSIDRGLFRSLFFRSYMDQEKRFGISIVEEFEKPTRATTLRLKHGTYEKLDEDGLIAPGVRVSGDDIIIGKTTPIPPDTEELGQRTKYHTKRDASTPLRSTENGIVDQVLLTTNQEGLKFVKVRMRTTKVPQIGDKFASRHGQKGTIGVTYRHEDMPFSAEGIVPDLIINPHAIPSRMTVAHLIECLLSKVGSIRGYEGDATPFTDLTVDAVSNLLRDNGYQSRGFEVMYNGHTGKKLMAQVFFGPTYYQRLRHMVDDKIHARARGPVQVLTRQPVEGRSRDGGLRFGEMERDCMIAHGAAGFLKERLMEASDAFRVHVCGICGLMSVIANLKKNQFECRSCKNKTNIYQLHIPYAAKLLFQELMAMNIAPRLYTERSGVSMRS c4r3p8_kompg DNA-directed RNA polymerases I, II, and III subunit RPABC1 PAS_chr3_0157 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MEDNNRIISRLWRSFRTVKEMAADRGYFISQEEMDQSLEEFRSKICDSMGNPQRKLMSFLANPTPEALEKYSDLGTLWVEFCDEPSVGIKTMRNFCLRIQEKNFSTGIFIYQNNITPSANKMIPTVSPAIIETFQESDLVVNITHHELVPKHIRLSDGEKSQLLQRYKLKESQLPRIQREDPVARYLGLKRGQVVKIIRRSETSGRYASYRICL c4r2u9_kompg PAS_chr2-2_0104 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MNVSTSTVGARRRRAKQQVDDEENATLLRLGPEFALKQYDHDGNEHDLIALSLSESRLLIREALKARSRARNGGVDIESSNGEIDDDELAKVTSGAVANGVVKKTLDYLNTFARFKDEETCTAVDQLLHNSSDCSVLHPFEIAQLSSLGCEDVDEAITLIPSLAAKKEVNLQRILDELNRLEDPYK c4r9a1_kompg PAS_chr4_0906 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MFFLKDLSLILTLHPSYFGPQMNQYLREKLLTDVEGTCTGQFGYIVTVLDGMNIDVGKGRIIPGSGSAEFEVKYRAVVWKPFKGEVVDAIVSNVSPIGFFADVGPLNVFVSTRLIPDNLVYNPSNSPPAYMSNDELITKGSKVRLKVVGTRTDVNEIYAIGSIKEDFLGAI c4r1v1_kompg PAS_chr2-2_0434 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MSEDEAFNEQTENFENFEDEHFSDDNFEDRSTQPEDYAVGVTADGRQIINGDGIQEVNGTIKAHRKRSNKELAILKEERTTTPYLTKYERARILGTRALQISMNAPVLVDIEGETDPLQIAMKELSQRKIPLVIRRYLPDGSYEDWGCDELIVDN h4_human Histone H4 H4-16 H4C1 H4C11 H4C12 H4C13 H4C14 H4C15 H4C2 H4C3 H4C4 H4C5 H4C6 H4C8 H4C9 H4/A H4/B H4/C H4/D H4/E H4/G H4/H H4/I H4/J H4/K H4/M H4/N H4/O H4F2 H4FA H4FB H4FC H4FD H4FE H4FG H4FH H4FI H4FJ H4FK H4FM H4FN H4FO HIST1H4A HIST1H4B HIST1H4C HIST1H4D HIST1H4E HIST1H4F HIST1H4H HIST1H4I HIST1H4J HIST1H4K HIST1H4L HIST2H4 HIST2H4A HIST2H4B HIST4H4 protein protein human Homo sapiens Human MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG h33_human Histone H3.3 H3-3A H3-3B H3.3A H3.3B H3F3 H3F3A H3F3B PP781 protein protein human Homo sapiens Human MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA h2b1j_human Histone H2B type 1-J H2BC11 H2BFR HIST1H2BJ Histone H2B.1 Histone H2B.r protein protein human Homo sapiens Human MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSAK h2a1b_human Histone H2A type 1-B/E H2AC4 H2AC8 H2AFA H2AFM HIST1H2AB HIST1H2AE Histone H2A.2 Histone H2A/a Histone H2A/m protein protein human Homo sapiens Human MSGRGKQGGKARAKAKTRSSRAGLQFPVGRVHRLLRKGNYSERVGAGAPVYLAAVLEYLTAEILELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLGRVTIAQGGVLPNIQAVLLPKKTESHHKAKGK f2qpe6_kompc DNA-directed RNA polymerase subunit RPB9 PP7435_Chr1-1144 protein protein kompc Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1) Yeast Pichia pastoris MASFRFCLECNNMLYPKEDKENQRLLYSCRNCDYTELAEDPKVYRHELITNIGETAGIVDDIGQDPTLPRSDKECPECHSRDCVFFQSQQRRKDTNMTLFYVCLNCKKTFRDESE c4r273_kompg DNA-directed RNA polymerases I, II, and III subunit RPABC3 PAS_chr2-2_0309 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MSSALFDDIFTVQTVDNGRYNKVSRIIGISTTNSAIKLTLDINNEMFPVSQDDSLTVTLANSLSLDGEDESANFSKSWRPPKPTDKSLADDYDYVMFGTVYKFEEGDEDKIKVYVSFGGLLMCLEGGYKSLASLKQDNLYILIRR c4r3z5_kompg PAS_chr3_0244 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MNAPDRFELFILPDDVPKLKITPDSRVPNCIIIKFEREDHTLANLLREELALYPDVTFVAYKVEHPLFANFVMRLQTEEGTRPKQALERACASIINKLKTLDHKFNEEWNIKNFSLND c4r009_kompg PAS_chr2-1_0223 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MIIPVRCFSCGKVVGDKWDAYLRLLEEGKQEGDALDELKLKRYCCRRMVLTHVDLIEKFLRYNPLEKKDFDS c4qz45_kompg Transcription elongation factor 1 homolog PAS_c121_0006 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MGKRKSSARKPAPKIKQKLETQFTCLFCNHDNSVVCTLDKKNSIGLLECKKCNLSFQAPINSLSQPIDIYSDWIDACEAVAEENADVNGDNFIENDGADREQDDDYDDEF f2qmi1_kompc RPC10 PP7435_Chr1-0476 protein protein kompc Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1) Yeast Pichia pastoris MSREGFVAPSGTDLAAAASGVAPNKHYGVKYTCGACAHNFSLNKSDPVRCKECGHRVIYKARTKRMIQFDAR c4r370_kompg Chromatin elongation factor SPT5 Chromatin elongation factor spt5 transcription Elongation factor SPT5 PAS_chr3_1136 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MSETHKNQLDKVSTVSPDGPSEAVKEHSLQSKDLSKNDGQFIVPLDRNVIEQEEHKQVKSSAQAHNTTGDAADNEIEDGVPSEDVEFDKFKEDDYDEDDEVEEEGDIRSRKRRRHNQFLDVEAEVDDEEDDDDDDDDVELKHDFIQDDHIQHETQNEGFIAGHVDDDRLHRKLDQSREKIADQDAQELADEFKQRYGRSASSKYMGSASTTAPQRLLIPTVDDPGIWGVKVRLGKEKDVVRQILKKKLAREGTKNPLEIYSAFQRDSFKGHVYIEARKAEAINDALKGNVNVFSNNSKFLVGIVEYKDLLRPVKSSDVKLTRGSYVRVKNGKFKGDLAQVDEVLENGLEARLKLVPRLDYGKDLSHLSTSSSVDSTKNRRKFYTSKFRPAQRLFSEAEARVHEPTIRRDRDGFVTYGGEEYYEGFLYKTFRLQNLIVNSINPTLNELSLFQSNEESTTIDLSTIADSLKETAKNLVSFQPGDNVEIINGELNHLTGTVSSVNQSTIVSVRLHSDDDTINSETVEIPTSDLRKIFNVGDHVRVIHGKHTDDTGLIVEVNGDKVEFISNQTKRTVIVFSNYLIKSTDSTVSINESGRFELHDLVQVNSDLVGIVIRAQKDSFDVLCSDGKLLSLPPVSIYSKLNLNPNQQIAIDSNGVEVKVGDTVREFTGERRQGTILHVYRNFLFLRSREIVENQGVFVTSSNRVKTITSKSNGTGGQISGPDLSRMNPSRVIPPPSIPVANQRMTGRDPTLNKTVKIRQGGYKGKIGIVKEANGDRFRVELHNPNKTIPIPCSFLLIESTHGWVPYEDFVASDRRGGNIPRHEISGHVQQPQHGRAPAWGSGGKTPAWGSGGKTPAWGSGGSGGKTPAWGSGGKTPTWGSGAKTPAWGSGSKTPAWSGLDEEDRRDF c4r0e6_kompg Transcription elongation factor SPT4 PAS_chr2-1_0350 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MSTNKGVRERACMLCGIVLPGRVFMQNGCPNCDSVLNLRDSDQATVNECTSSSFEGLVAVGDNEHSWVAKWLRVDRFQPGLYAVRVDGRLPSDIVAALEQYGVYYRPRDGSVID c4qy79_kompg DNA-directed RNA polymerase subunit PAS_chr1-4_0359 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MTNVNSLSNNMLYPKEDKENQRLLYSCRNCDYTELAEDPKVYRHELITNIGETAGIVDDIGQDPTLPRSDKECPECHSRDCVFFQSQQRRKDTNMTLFYVCLNCKKTFRDESE c4r2r6_kompg PAS_chr2-2_0135 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MDAKEIVTVMKDLEKAESDSQIIRLLTLLQTDVVPTEKVLRETKVGVTVNRLRSHANPEVGTLVKKIIKTWKDGVSQEKKKKAVSSAGSPAKSTTSTTTNERFVSKGPRTPKNDGVKVEIYENKTRNGSISAIYTALAMDSDEQPSKIFELVKDIEKQCFKAVNFTVDDTYRNKLRSLIMNLKNKNNPTLRRSILDHEIIPSKLVTMSAQELAPDSLKKEMEEIYKKNLFDAQGATENNSVTDRFECGKCKQRKVSYFQKQTRSADEPLTTFCKCENCGNRWKFS c4qwa8_kompg PAS_chr1-1_0166 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MSREGFVAPSGTDLAAAASGVAPNKHYGVKYTCGACAHNFSLNKSDPVRCKECGHRVIYKARTKRMSKFLTTY f2quc3_kompc Chromatin elongation factor SPT5 SPT5 Chromatin elongation factor spt5 transcription Elongation factor SPT5 PP7435_Chr3-0027 protein protein kompc Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1) Yeast Pichia pastoris MSETHKNQLDKVSTVSPDGPSEAVKEHSLQSKDLSKNDGQFIVPLDRNVIEQEEHKQVKSSAQAHNTTGDAADNEIEDGVPSEDVEFDKFKEDDYDEDDEVEEEGDIRSRKRRRHNQFLDVEAEVDDEEDDDDDDDDVELKHDFIQDDHIQHETQNEGFIAGHVDDDRLHRKLDQSREKIADQDAQELADEFKQRYGRSASSKYMGSASTTAPQRLLIPTVDDPGIWGVKVRLGKEKDVVRQILKKKLAREGTKNPLEIYSAFQRDSFKGHVYIEARKAEAINDALKGNVNVFSNNSKFLVGIVEYKDLLRPVKSSDVKLTRGSYVRVKNGKFKGDLAQVDEVLENGLEARLKLVPRLDYGKDLSHLSTSSSVDSTKNRRKFYTSKFRPAQRLFSEAEARVHEPTIRRDRDGFVTYGGEEYYEGFLYKTFRLQNLIVNSINPTLNELSLFQSNEESTTIDLSTIADSLKETAKNLVSFQPGDNVEIINGELNHLTGTVSSVNQSTIVSVRLHSDDDTINSETVEIPTSDLRKIFNVGDHVRVIHGKHTDDTGLIVEVNGDKVEFISNQTKRTVIVFSNYLIKSTDSTVSINESGRFELHDLVQVNSDLVGIVIRAQKDSFDVLCSDGKLLSLPPVSIYSKLNLNPNQQIAIDSNGVEVKVGDTVREFTGERRQGTILHVYRNFLFLRSREIVENQGVFVTSSNRVKTITSKSNGTGGQISGPDLSRMNPSRVIPPPSIPVANQRMTGRDPTLNKTVKIRQGGYKGKIGIVKEANGDRFRVELHNPNKTIPIPCSFLLIESTHGWVPYEDFVASDRRGGNIPRHEISGHVQQPQHGRAPAWGSGGKTPAWGSGGKTPAWGSGGSGGKTPAWGSGGKTPTWGSGAKTPAWGSGSKTPAWSGLDEEDRRDF a0a1b2jer8_picpa Transcription elongation factor 1 homolog ELF1 ATY40_BA7504229 protein protein picpa Pichia pastoris (Yeast) Yeast Pichia pastoris MGKRKSSARKPAPKIKQKLETQFTCLFCNHDNSVVCTLDKKNSIGLLECKKCNLSFQAPINSLSQPIDIYSDWIDACEAVAEENADVNGDNFIENDGENREQDDEYDDEF ei2be_schpo Probable translation initiation factor eIF-2B subunit epsilon tif225 eIF-2B GDP-GTP exchange factor subunit epsilon SPAC8C9.15c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPPSKGLNGKLEKPKHALQAIVLSDSYNYRFRPLTLDKPRCLLPLANTPLIEYTFEFLALAGVQEVYVFCCAHAGQIREYIEKSKWNLPSSPFSVNTIVSRESLSVGDALRELDSKQLITSDFILVSGDVVSNVPLNEVLKEHRKRREDDKNAIMTMVVREASPFHRTRARTESSVFVIDKKTSQCVHYQANERGKHYVSMDPEIFNEHEELEVRNDLIDCQIDICSNDVPALFTENFDYQDIRKDFVYGVLTSDLLGKKIHCHVAKENYAARVRSLQTYDAISKDVLSRWVYPFVPDSNLLNQTFSYQRHQIYKEEDVVLARSCIIKARTLIGAYTKVGDASVVANTIIGRNCTIGSNCSIDSAFLWEDVVIGDNCRIGKAILANSVKIGNNCSIEDGAIVAAGVVIGDNTIIEKNKRLTTFESHSQGTLNDPSLVGIGGRGQEYHAEEDSDDEGEFMEASGLIESTNELHLSDSESSETSSSSEEDMEFIPFSARRDSANTINSEDFDEGDFNKEAQQSLERAFEENHQIDIAALELNTLRMAMNANYHEVRSAIVLALLRRIMHLDVSPKEALAKVMTRWGPLLAKLTFSHEEQVDNVLTLQKYCVRLSMTRHFLQLLGYFYQLEIAEENAIQEWYSDPRSSEGELAALRDAGGKQFVDWLNTAESESESEEGSE D399547CBD82C4BC ei2bb_schpo Probable translation initiation factor eIF-2B subunit beta tif222 eIF-2B GDP-GTP exchange factor subunit beta SPAC343.14c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSTINVEHTYPAVSSLIADLKSRKVQGPFAVAVETALVMRQVISQTRWSTVDQLIDTVRAVGSTLVKAQPTEFSCGNIIRRILRLIREEYQELLKTADENEKLIVSSSNSSSPSQKRDIPSNEKLVQSHEPVSVQMYSSMLNLLGRPTLESPTHSKTVGDSRVTGGMDMRAVIISGIQDVIDELDKINTDIEVQSMDHLHSNEIILTQGCSKTVEAFLRFAAKKRKFSVIVAEGFPNNQKGSHAMAKRLAQAGIDTTVISDATIFAIMSRVNKVILGTHAILGNGGLVTYSGAQLVAQAARHHATPVVVCSGIYKLSPVYPYDLESIIQLSSPDKIMSFNEGDLISRAEILNPYYDYIPPDLVDLFITNLGGYPPSYLYRIMNDTYDASDTIL 19F324EF0135628B ei2ba_schpo Translation initiation factor eIF-2B subunit alpha tif221 SPCC11E10.07c eIF-2B GDP-GTP exchange factor subunit alpha protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MVESDSSGIVRHSQGEFDIVQVYKKFLQDDPEITMPVAAIEALVQLLSRSQAKTISEFMDILQNGSNTLKEGVQNNISLSAGCDIFQRFVTRSLHDVGDFEQCKRHLVENGKLFIQRARACRQRIAHLGYPLIRDGSVILTHGFSRGVAAVLLAAAKRHVRFKVFVTESRPSGSGCLMTRTLKNACIPTCMVLDSAVSFTMNRVDLVLVGAEGVVENGGLINQIGTFQLAVFAKHAHKPFYAVAESHKFVRMFPLSQYDIPFSRPILEFDDPSPETVHPEPEPIPTPSDAIHNELIMNEEQIRNNPTLDVTPPEFVSGLITDLGIIDSKSGVSEELIKLYL 6FAACEBEBD50995A ei2bd_schpo Probable translation initiation factor eIF-2B subunit delta tif224 eIF-2B GDP-GTP exchange factor subunit delta SPAC21E11.06 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MGFSAEQAKKDGKDQSPVSESSSVGGTSPATASSVVSPNEPKLSGKEAKALKKARKQASRRAKAEAAAANNPPGVSEEKKVAIPNKNSNQQKKASKQNPQNSPETDANLQEKKIFEEKQVSIFSHLDWRRRRTTENIPKDIHPAVIRLGLKLANYKIFGSNQRCIDLLKTFKIVIQDYQTPYGTTLSRHLTTHINSQIAYLVSTRPLSISMGNAIRFLKLEISVLDIDLTDDEGKELLLEKIDSYIRDRIIIAGQVIVQAATEKIQDGDVILTYLHSSTVNDVLIHAKNVGKKFRVVVVDSRPEFEGRVCLKLLTEHGIECTYVMISALSYIMQEVTKIFLGGHAMLSNGALYSRAGTSLISLLGHESNVPVIACCESYKFTERIQLDSLVYNELAPGDQLVNMGVDDFEEKPGVLANWKSVKNLKLLSLKYDVTPPRLITVCVCEMGLLPSTSVPAIINEFKQVYA 3C7029EBDFEF6EAF ei2bg_schpo Probable translation initiation factor eIF-2B subunit gamma tif223 eIF-2B GDP-GTP exchange factor subunit gamma SPAC4D7.09 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSLYEHAALPLASSPSILGPISGGRNRGNIQLQSIPIEFQAVVFAGFGNSLYPLTGSDALPKALLPIGNKPMLHYPLYWLEAAGFTSAILICMEEAEAHINAWLRSGYEGHMRIHVEAPTILDDSKSSADALRAVSHLIKNDFVCLSCDSIVGLPPIYGLDKFRLDNPSALAVYSPVLKYEHITSQSKEIDAKQLIGIEEKTSRLLYAKSSADVGSDFTFRMSLLWKHPRVTLNTNLSDAHIFVFKHWVIDLIREKESISSIRGDLIPYLVKCQYQKSFTVRENIQRFLSSPNNIDNYDGGLSSQEIKINALIAKDGIICSRANNLPNYFELNKCIAKLTPEQRLVDVTVSERALVGADCMVNEGTTIKDNSNIKKSIIGKNCVIGKGVVVSNSILMDNIVVEDGVRLESCIVASGAQIGAKSKLRECEIGVDHRVEAGRIARGERLVDMEKIETDMD 242987543BE43021 c4qxj0_kompg PAS_chr1-4_0135 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MSEEITKTEALGFDPTLKKKKKKAKSADPEVLEQSVDTKEGDDDLFSGLKKKKKKATAATGEEKEVEKDLADVMGELKLKKKKKKAAAPVSEDFDKLLEKAGVLEDDKDVTKESTPEVETTTDLGPKYEDMLSRFFKVLKQNNPELAGERSGPKFRIPPPVVQREGTKRTLFANVQEIATVLQRSPEHLIQYLFAELGTSGSIDGQKRLIIKGRFQPKQIENVLRRYIIEYVTCKTCKSMNTELKRESANRLHFIVCKACGSTKSVSSIKTGFQAHVGRRRKM c4r3h4_kompg PAS_chr3_0077 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MSTSTCRFYENKYPEVDDVVMVNVQQIAEMGAYVKLLEYDNIEGMVLLSELSRRRIRSIQKLIRVGKNEVVVVLRVDKEKGYIDLSKRRVSSEDIAKCEERYTKSKSVHSILRHCAEKFNMPLEELYRTIGWPLSKEFGHAYDAFKISITDSTVFDKVQPPSQEVLEELKVYISRRLTPQAIKCRADVEVSCFSYEGIEAIKSSLKAAEDISTEANQVKVKLVAAPLYVITTQSLDKTQGIEILERAIKIIEDSITKQGGSCNVTMAPKAVTATEDAELQALMERKENEDKVSDEDSDLDEED c4r5q4_kompg Protein-synthesizing GTPase PAS_chr3_0839 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MADDLQSQTPDIIIGSTIDDGYEQYATEEEIEEDQTQDLDESKSKKTVSFAELDEVDEEELRKKEFEEGGGLPAQPDNPEFEKLTPLSPEIINRQATINIGTIGHVAHGKSTVVRAISGVQTVRFKDELERNITIKLGYANAKIYKCDNDECPEPDCYRSFKSDREIHPKCQRPGCTGRYNLVRHVSFVDCPGHDILMSTMLSGAAVMDAALLLVAGNESCPQPQTSEHLAAIEIMKLNHVIILQNKVDLMREETALEHHKSILKFIRGTIADGAPIVPISAQLKYNIDAVNELIVKTIPVPPRDFTASPRLIVIRSFDVNKPGAEIDELKGGVAGGSILTGVFKIGDEIEVRPGIVTKDDSGKIQCKPIFSSIVSLFAEHNDLKFAVPGGLIGVGTKVDPTLCRADRLVGQVVGAKGHLPSIYTDIEINYFLLRRLLGVKTDGQKQAKVRKLDPNEVLMVNIGSTATGARVVAVKADMARLQLTSPACTEINEKIALSRRIEKHWRLIGWATIKKGTTLEPIA c4qxj0_kompg-c4r3h4_kompg-1 3289718 N/A 3289733 unspecified role unspecified role unspecified role unspecified role picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris N/A 3289734 unspecified role unspecified role unspecified role unspecified role picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris N/A 3289735 unspecified role unspecified role unspecified role unspecified role picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris physical association physical association false false false c4r5q4_kompg-eif2bepsilon-1 3289719 N/A 3289733 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris N/A 3289727 unspecified role unspecified role unspecified role unspecified role pBpa-label pBpa-label unnatural residue unnatural residue certain certain sequence position certain certain certain sequence position certain false pBpa-label pBpa-label unnatural residue unnatural residue certain certain sequence position certain certain certain sequence position certain false pBpa-label pBpa-label unnatural residue unnatural residue certain certain sequence position certain certain certain sequence position certain false pBpa-label pBpa-label unnatural residue unnatural residue certain certain sequence position certain certain certain sequence position certain false pBpa-label pBpa-label unnatural residue unnatural residue certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false c4r5q4_kompg-eif2bgamma-1 3289721 N/A 3289729 unspecified role unspecified role unspecified role unspecified role pBpa-label pBpa-label unnatural residue unnatural residue certain certain sequence position certain certain certain sequence position certain false pBpa-label pBpa-label unnatural residue unnatural residue certain certain sequence position certain certain certain sequence position certain false pBpa-label pBpa-label unnatural residue unnatural residue certain certain sequence position certain certain certain sequence position certain false pBpa-label pBpa-label unnatural residue unnatural residue certain certain sequence position certain certain certain sequence position certain false pBpa-label pBpa-label unnatural residue unnatural residue certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3289733 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris physical association physical association false false false c4qxj0_kompg-c4r3h4_kompg-2 3289722 N/A 3289733 unspecified role unspecified role unspecified role unspecified role picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris N/A 3289734 unspecified role unspecified role unspecified role unspecified role picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris N/A 3289735 unspecified role unspecified role unspecified role unspecified role picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris N/A 3289727 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3289728 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3289729 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3289730 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3289731 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false c4qz45_kompg-c4qzq7_kompg-1 3544067 N/A 3544068 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3544069 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3544070 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3544071 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3544072 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544073 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544074 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544075 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544076 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544077 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544078 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544079 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544080 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544081 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544082 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544083 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544084 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544085 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544086 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 physical association physical association false false false Figs. 1 and S5 and S6, Table S1 and S2 Identifier F2QTN1 in PDB accession code 6J4W has been changed to C4R009 to be consistent with the rest of the PDB accession codes and since they code for the same protein. c4qzq7_kompg-c4r009_kompg-2 3544067 N/A 3544068 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3544069 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3544070 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3544071 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3544072 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544073 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544075 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544076 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544077 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544078 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544079 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544080 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544081 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544082 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544083 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544084 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544085 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544086 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 physical association physical association false false false Figs. S12, Table S1 and S2 Identifier F2QTN1 in PDB accession code 6J4W has been changed to C4R009 to be consistent with the rest of the PDB accession codes and since they code for the same protein. c4qzq7_kompg-c4r009_kompg-3 3959116 N/A 3959118 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3959119 unspecified role unspecified role neutral component neutral component purified purified A0A1B2JER8:p.[Asn53Gly;Leu54Gln;Ser55Arg] p.[N53G;L54Q;S55R] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false binding region binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544075 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544076 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544077 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544078 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544079 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544080 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544081 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544082 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544083 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544084 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544085 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544086 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 physical association physical association false false false Fig 1, Fig S3, Table S1 c4qwa8_kompg-c4qy79_kompg-1 3959117 N/A 3544072 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544073 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3959134 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3959135 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544076 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544077 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3959136 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544079 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544080 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544081 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544082 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544083 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544084 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544085 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3544086 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 physical association physical association false false false Fig 2, Fig S6, S10, Table S2 c4qzq7_kompg-c4r009_kompg-1 3493437 N/A 3544075 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544076 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544077 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544078 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544079 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544080 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544081 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544082 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544083 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544084 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544085 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544086 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified picpa Pichia pastoris (Yeast) Yeast Pichia pastoris Stoichiometry: 1.0 N/A 3544068 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3544069 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3544070 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3544071 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 physical association physical association false false false Fig 1A, Table S1-S2 MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint esaki-2006-2 The budding yeast Pichia pastoris has a novel Sec23p homolog. yeast Saccharomyces cerevisiae Baker's yeast two hybrid array two hybrid array predetermined predetermined participant predetermined Esaki M., Liu Y., Glick BS. FEBS Lett. (0014-5793) 2006 2009-01-08 Only protein-protein interactions imex curation esaki-2006-1 The budding yeast Pichia pastoris has a novel Sec23p homolog. picpa Pichia pastoris (Yeast) Yeast Pichia pastoris fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Esaki M., Liu Y., Glick BS. FEBS Lett. (0014-5793) 2006 2009-01-08 Only protein-protein interactions imex curation sar1_kompg Small COPII coat GTPase SAR1 SAR1 PAS_chr1-1_0180 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MWVLNWFQDVLASLGLWNKHAKLLFLGLDNAGKTTLLHMLKNDRLATLQPTWHPTSEELSIGNVRFTTFDLGGHEQARRVWKDYFPEVDGIVYLVDIADPERFEESRVELDALLKIEELSKVPVLVLGNKIDKSTAVSENELRHALGLMTTTGKDKVQLVEGQRPLEVFTCSIYLRQGYGEGIRWLSQYI mint Small GTPase component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules. SAR1 controls the coat assembly in a stepwise manner. Activated SAR1-GTP binds to membranes first and recruits the SEC23/24 complex. These SEC23/24-SAR1 prebudding intermediates are then collected by the SEC13/31 complex as subunits polymerize to form coated transport vesicles. Conversion to SAR1-GDP triggers coat release and recycles COPII subunits sec13_kompg Protein transport protein SEC13 SEC13 PAS_chr1-3_0057 protein protein picpg Komagataella pastoris (strain GS115 / ATCC 20864) Yeast Pichia pastoris MVTIGNAHDDLIHDAVLDYYGRRLATCSSDKTIKIFEIDGENQRLVETLIGHEGPVWQVAWAHPKFGVILASCSYDGKVLIWKEDNGVWNKVAEHSVHQASVNSVSWAPHEYGPVLLCASSDGKISIVEFKDGGALEPIVIQGHAIGVNAASWAPISLPDNTRRFVSGGCDNLVKIWRYDDAAKTFIEEEAFQGHSDWVRDVAWSPSRLSKSYIATASQDRTVLIWTKDGKSNKWEKQPLTKEKFPDVCWRASWSLSGNVLAISGGDNKVTLWKENIQGKWESAGEVDQ mint Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules. It also functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. SEC13 is required for efficient mRNA export from the nucleus to the cytoplasm and for correct nuclear pore biogenesis and distribution q9p4c6_picpa SEC12 protein protein picpa Pichia pastoris (Yeast) Yeast Pichia pastoris MMKPYTLDTGYPVYGAKFITKRTLLTAGGGGEGNNGIPNKLSGFRIDFTKVKAVQKFRELTLSANEDCPMSLDAANNVILLGVNENTSSIKQGKNNHLRKFGYINHHLKYLDKQQLSNSRDNRDYQKFTHLSSDASVACIATSKVPTTIYVVDPQTLEKKFDLETGVDVKDMHISPDGKIVCYVCANSLHGYSTVTAKLLFKDDSFKNHTLMKVKFLDQHHLLIVGSQKQGISLIHYSLAKNSIVNSRVISKKLKGVTSLDTRNGVIALSGNDNSLLLIKVSNLKPIKQFNKIHKFSITSCCFNKTGDLLATVSAANTVSVMEIPKGLATKKSLPRKIFNYFILTVFMAILAIVLQWSIENGHLQHAWQKLLNGDVIDSSRYFKVESIPDEELSASLLESSYSGLSSETKSVTDAISASVPIIETTVDTTTTSNSVRRTIPEGYSTAKEFQPDKIKLYKSSLDSDPSADSTGSFTVSITETQSSSIKPQKSKKTKKLKKKTSSTATPGSSTALVETLIVSTSPQSVDTSLSVTSPVASSTAQDSYLTSSAHSALTTKGAKKLKKKRKIKTKTVKTTKTTETETETETIVQDDLADITISSDIVSDISSEVSNSETVVQEGLPTEVIESLLNKDPTVDEPIAEESAIIDLPLGKPVTEKLTLDTQEAIESSVDEPAIGIDIEDAIVADEKEEPLTADDAVLDGEEREIEDPDEIPSEVLTDVADSPEEYQDDININNSQEATSTHDSNVSEETAAPVEETQESVGTDDQAEPEKFDEEEKTENEHQYEKDDHSELAEETQESHDDQVDEVEEVLEEVKDFLGETKDVLEEAKFVLEEAQEGAEVSDKTELTEQDETEKFIESSDALRSSVGTVEATEQTTATSVDPNTEIDTKTTSEAVIESNTPVENEESTVEDREINSSIDEIAEKSISQEEIVSKLEDSSSENNEGEREEEEIEESEDEKAGDIEEEEEEYEDDDDEYENVGTEEEKDEEEVQGDHEEQEDEEEVDDEEEEDDDEDEEDEDEDEDEEGENINHDEL mint q0pve0_picpa Protein transport protein SEC23 SEC23 protein protein picpa Pichia pastoris (Yeast) Yeast Pichia pastoris MADIYEIEEIDGVRFNWNSFPVTKIESENISMPLGCLYTPLNERDYLPVAQYDPVLCRQCHAALNPYAMIDLTAKVWSCPVCSSRNTLPINYTEISTENLPLELNPQSSTIEYILSRAQPHPPPAYIYVVDLCQEQSELDSMKETLIASLPLHPPGALIGLVTFDSVVNVHDLSTRTCLKKYVFNGTKEYTSIEIQRQLGIEKSGTIPWAKQLQNPGYNAVTRFFLPVAEKDAEFQIIRAIERLEVSKWTYPQGQRPTRVTGAALSVATSLAEGSYADCACKITLFSAGPCTLGPGTIVGPELKEPIRSHNDMDKGTASHYKKSYQFYNRLAVKGSNNRKDLKDKFADPSSSTTYSVDIFAGCYDQVGIYEMRSLANLTGGVLVVTDSFQTSILKNSFFACFNKDEEGYPMTYSDGILDVLTSNRLKVSGVIGHAVSMKQNGNNISDTPIGEGLTNKWRLCSLTPKNTYAIFFDMMTVGTSDQRSSSVNEVCIQFTTTYRHTNGQMRTRVTTLKRGTSNTVPLANSFDQEAAAVLYARLIVNKIENGNEYSDLLRWIDKSLVKLCTVFGDYSKNDAGTFRLSTKFSLLPQFIYHLRRSQFLQVFNCSPDETAFYHHTILRTDIRDSLVMIQPTLTQFSADGSEPVPVLLDSASLKQDCVLLLDAFFFIVIYYGSVAAAWRDANYPREEFPGVYAMIEKAQEEAASLISDRFPLPKYVTTDEGKSQARFLYSKLNPSENDTETTGSALAGYTASAHEAIVQSDDVSLNTFFTHLSRLVVQNSS mint sec24_picpa Protein transport protein SEC24 SEC24 protein protein picpa Pichia pastoris (Yeast) Yeast Pichia pastoris MSGKRRAYPSMQYPATEPVATGGSFGYGQQPGAQAYGQQFTPGAGIAGQQLPGATQPNLNGQGAPYGAPGGDQLAQGMSSLNIQPNQQPQPVHQFPSQQQGYINPGAGLGVPPTQAAGPYGVPAAVAPQQQSAQLGYAGPPYGNTYDQQQPQQAAFPFNQLYTADLLKELPPPISDLELPPPPIVLPQGASLTGKPESNASPEYFRCTLNVIPNNNSLLKKSKLPLAVVVNPYQCLRDEDEPVPVVEDTLISRCRRCRSYINPLITFVDRNTKWRCNLCNLTNDVPSGFDFDKDTQQRVDRMARVELNYSVVEFVAPKEYMVRLPQPLVYLFVLDVSTHAIQNGYLATVARTILDSLDQIPNKDGRARVGFIGVDSSLHFFTIPQDSEDENDAQETSMLVVSDLDDVIVPAAENLLAPLQQSRQNIENLLNNFHSYFENNVNPNFALGPALKAGHRLINNIGGKMIVFTSTLPNKGIGALSIRDEEAHSGKAKESSALLSPNDSFYKSFAVECNKSQVTVDMFLASSSYQDVATLSNLPRYTAGQTHFYPAWTAAREEDITKLSKEISNHLSMNINLEAVLRVRGSAGLRMNAFYGNFFNRSSDLCSFPTFPRDQSYLIEISIDEHITKPLAAFQAAVLHTTSFGERRIRVMTLEVPIGKELNQIYASADQLAITNYFTHKAVEKALSSSLIDAREYLNRSLLDIFQVFKKELVAGNLGSSSPLQLCNNLKMLPLLLHSLTKYIAFRPGKVPSDHRAYALNLLASSPIQRLIKFIYPTIYSLHDMADECGLPEELEETYVNEAGEEVTEPIDGDIVLPEPINDTSTLLAPYGLYLIDSGTDLFLYVGGESVPQLLLDVFGVDNPGYIKYGKSELPELNNEFNERLRNVINRVREGKDRITYQNLQIVIGQSKQRTGVPDRIQEDLTPLRLWCFSHLVEDRVGGGTAYREYLNQIREKLSS mint Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules q0pvd7_picpa LST1 protein protein picpa Pichia pastoris (Yeast) Yeast Pichia pastoris MEATHSMNAVYDGYSMSSLSDGMSELKVDQSKHKKRPVHILHQQPASQTTAQFHQPASSQPFLPPQVPFSQQPQSPNFVQGSPQIGTPQMGTPPLGVSQFGTPQGQIQSPGQLHQTFSPPSNLDPTSVPDIRYETDVMFDSKSFLTFANVCPPLVGTQYTVVDQGNASPRFMRMSMYNVPATEKLRKETGLSLGLVLNPFAPMQEQEKPIPEVDYREMGGPPRCRRCRAYVNSTMQHTHDYRMICNICGFSSPIPSEYCSPLDPSGVRIDRNQRPELHLGMIDFLVPEAYNIDESIPPNPLHHVFLVDISNSSVRRELHKAFANAISMSVLKGYPKGSKISIIAYDRKIHFFNLSLDSNQVSIATVSDLEDPFLPFFDGIFVDVKAGRIHIDRALQSLEMMGEYSEPEPAYGAALRYALLALEHVGGGKVTALLSALPSWGPGSLKGISPSQRLTSELETEALTAKNQYYQKLSKDYISASVGLDLFVVSSGSVDLSDCSLVATSTGGSVRSFFNYEVARDQKSLCISLLDSIQAVKGYQSQLKIRCSAGLQVTKYLGSFKTSNSDADPLIPIVTSNTSVGCLFKHDGKLSTKNDAHFQAALLYTSADGVRKVRVINSIISVTERIADVFAFADQDATLALELKEHLGRIPEVSLVGLRNRLTFDLVDINTDYKLKVVGKSSADQQLILPVGLRTLPMFTLAATKTRAIRSAPSNNTDLRVQSILDLKSFTLEKLAVYLYPILINVLEIDEDACQYTSTGHFQLPPGTRLSRQNLMHGGAYILFNGLKVFFWIHSDVNVLLLQDVFGQNVSSLDSIDPNLNELPELDTGISLQLRNLVSFFAKSYLNSSFVPIQICRFRRDGAEVEFMENLYEDSTPDKLQSYHDFLNHIHSLIKNKVAVSSEKKNQMNGQMMEMNHLR mint q0pvd9_picpa Protein transport protein SEC23 SHL23 protein protein picpa Pichia pastoris (Yeast) Yeast Pichia pastoris MDQDAFETIEDIDGVRFTWNAFPRTKIEAARCCVPIACIYTPLKQRENLAIIQEDPMICRACKAIFNPFVTVQAQHWICSFCATLNVLLPHYQNQIPYQASGDCTTIEYVLSKRVSSPPAYVYLVDTSLEAQELESLKESILNSFQLLPKYALVSLITFGRNVNVHELEDGLAHNYYTFNGSKTYDLTSIQRSLGLLSRDLRRKDASESVGGRFLKPLGVCEFSLSEVVQSLKPDVWPKGKFQRSLRATGAALDVALHFMDTCLGKTGAHLMLFAGGPCTLGPGIVVGSELKEPIRSHYEIQNGSAKHYKKSLKHYAALAKRATSQCHSVDVFAASYDQIGLSEMELLVDQTGGALVLTDAFSTAIFKQSFQRFLQLPMGINGTLEVKTSFDLKINGLVGHAISLERKAKNVSDTEIGRGKTDAWKLCNLLPQSSFGIYFEMPATNSSQTTTKPRSLIQFITYYQEIGGTLRLKVTTLAKPILQQSQESLIAEYFDQEAAVVLISRQMIDKMVKDNSTEVIRELDKILVELCKKFGSYQKNDLQSFRLGAKFSLFPQFLYHLRRSQFMQVFNNSPDETTFYRHSILTEDTFNSLIMIQPTLTAYEINQEPQPIFLDSMSITADRILLLDTFFQIVIYHGETIVQWREAKYQEQEEFAYLKDFFQLPREDAADLLIDRFPLPRFIDCDAGGSQARFLLSKVNPTTSYKDNQMASASQSGGAIVGSDDVSLQSFMEHVQMVVVKSV mint sec23-sec23 544146 q0pve0_picpa 544148 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 q0pve0_picpa 544148 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F6 mint sec23-sec24-4 544146 q0pve0_picpa 544148 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 sec24_picpa 544149 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F6 mint sec23-lst1 544146 q0pvd7_picpa 544150 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 q0pve0_picpa 544148 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F6 mint shl23-lst1 544146 q0pvd7_picpa 544150 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 q0pvd9_picpa 544151 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F6 mint sec24-sec23-1 544146 sec24_picpa 544149 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 q0pve0_picpa 544148 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F6 mint lst1-sec23 544146 q0pve0_picpa 544148 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 q0pvd7_picpa 544150 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F6 mint lst1-shl23 544146 q0pvd7_picpa 544150 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 q0pvd9_picpa 544151 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 physical association physical association false false false F6 mint sar1-shl23 544146 q0pvd9_picpa 544151 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 sar1_picpa 544152 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false undefined mutation mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 physical association physical association false false false F6 mint sar1-sec23-2 544146 q0pve0_picpa 544148 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 sar1_picpa 544152 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false undefined mutation mutation increasing interaction mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false Stoichiometry: 1.0 physical association physical association false false false F6 mint sec13-sec23 544147 q0pve0_picpa 544148 immunostaining immunostaining unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level gfp tagged gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 sec13_picpa 544153 immunostaining immunostaining unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 colocalization colocalization false false false F4A mint sec13-shl23 544147 q0pvd9_picpa 544151 immunostaining immunostaining unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level gfp tagged gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 sec13_picpa 544153 immunostaining immunostaining unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 colocalization colocalization false false false F4A mint lst1-sec13 544147 q0pvd7_picpa 544150 immunostaining immunostaining unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level gfp tagged gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 sec13_picpa 544153 immunostaining immunostaining unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 colocalization colocalization false false false F4A mint sec13-sec24 544147 sec24_picpa 544149 immunostaining immunostaining unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level gfp tagged gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 sec13_picpa 544153 immunostaining immunostaining unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 colocalization colocalization false false false F4B mint sec12-sec13 544147 q9p4c6_picpa 544154 immunostaining immunostaining unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 sec13_picpa 544153 immunostaining immunostaining unspecified role unspecified role neutral component neutral component living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level gfp tagged gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 colocalization colocalization false false false F5 mint