DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ kranzusch-2011-1 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kranzusch PJ., Whelan SP. DIP original experiment: DIP-249730X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Arenavirus Z protein controls viral RNA synthesis by locking a polymerase-promoter complex. Kranzusch PJ., Whelan SP. Only protein-protein interactions imex curation kranzusch-2011-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Kranzusch PJ., Whelan SP. DIP original experiment: DIP-249731X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Arenavirus Z protein controls viral RNA synthesis by locking a polymerase-promoter complex. Kranzusch PJ., Whelan SP. Only protein-protein interactions imex curation kranzusch-2011-4 in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined Kranzusch PJ., Whelan SP. DIP original experiment: DIP-249734X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Arenavirus Z protein controls viral RNA synthesis by locking a polymerase-promoter complex. Kranzusch PJ., Whelan SP. Only protein-protein interactions imex curation kranzusch-2011-5 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Kranzusch PJ., Whelan SP. DIP original experiment: DIP-249732X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Arenavirus Z protein controls viral RNA synthesis by locking a polymerase-promoter complex. Kranzusch PJ., Whelan SP. Only protein-protein interactions imex curation abraham-2010-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Abraham J., Corbett KD., Farzan M., Choe H., Harrison SC. DIP original experiment: DIP-217039X Nature structural & molecular biology 2010 IMEx Structural basis for receptor recognition by New World hemorrhagic fever arenaviruses. Abraham J., Corbett KD., Farzan M., Choe H., Harrison SC. Only protein-protein interactions imex curation q6uy77_machu RING finger protein Z Z Zinc-binding protein protein protein machu machupo virus MACV MGNCNKPPKRPPNTQTSSNQPSAEFRRTAPPSLYGRYNCKCCWFADTNLITCNDHYLCLRCHQTMLRNSELCHICWKPLPTSITVPVEPSAPPP l_machu RNA-directed RNA polymerase L L Large structural protein Replicase Transcriptase protein protein machu machupo virus MACV MDEYVQELKGLIRKHIPDRCEFAHQKVTFLSQVHPSPLLTEGFKLLSSLVELESCEAHACQANTDQRFVDVILSDNGILCPTLPKVIPDGFKLTGKTLILLETFVRVNPDEFEKKWKADMSKLLNLKHDLQKSGVTLVPIVDGRSNYNNRFVADWVIERMRWLLIEILKASKSMLEIDIEDQEYQRLIHSLSNVKNQSLGLENLEHLKRNSLDYDERLNESLFIGLKGDIRESTVREELIKLKMWFKDEVFSKGLGKFKLTDRRELLESLSSLGAHLDSDVSSCPFCNNKLMEIVYNVTFSSVERTDGAATVDQQFSTTHTNIEKHYLSVLSLCNKIKGLKVFNTRRNTLLFLDLIMVNLMVDISESCQDAIESLRKSGLIVGQMVMLVNDRVLDILEAIKLIRKKIGTNPNWVKNCSKILERSHPEIWLQLNTLIRQPDFNSLISIAQYLVSDRPIMRYSVERGSDKICRHKLFQEMSSFEQMRLFKTLSSISLSLINSMKTSFSSRLLVNEREFSKYFGNVRLRECYAQRFYLAESLVGFLFYQKTGERSRCYSVYLSDNGVMSEQGSFYCDPKRFFLPVFSDEVLAGMCEEMTSWLDFDTGLMNDTGPILRLLVLAILCSPSKRNQTFLQGLRYFLMAFANQIHHIDLISKLVVECKSSSEVVVQRLAVGLFIRLLGGESDASSFFSRRFKYLLNVSYLCHLITKETPDRLTDQIKCFEKFIEPKVKFGCAVVNPSLNGKLTVDQEDIMINGLKKFFSKSLRDTEDVQTPGVCKELLNYCVSLFNRGKLKVSGELKNNPFRPNITSTALDLSSNKSVVIPKLDELGNILSTYDKEKLVSACVSSMAERFKTKGRYNLDPESTDYLILKNLTGLVSAGPKAKSSQEELSLMYETLTEEQVESFNEIKYDVQVALAKMADNSVNTRIKNLGRADNSVKNGNNPLDNLWSPFGVMKEIRAEVSLHEVKDFDPDVLPSDVYKELCDAVYKSSEKCNFFLEEVLDVCPLGLLLKNLTTSSYMEEEYFMCFKYLLIQGHFDQKLGSYEHKSRSRLGFTDETLRLKDEVRLSIRESNSEAIADKLDKSYFTNAALRNLCFYSEDSPTEFTSISSNSGNLKFGLSYKEQVGSNRELYVGDLNTKLMTRLVEDFSEAVGNSMKYTCLNSEKEFERAICDMKMAVNNGDLSCSYDHSKWGPTMSPALFLALLQMLELRTPVDRSKIDLDSVKSILKWHLHKVVEVPINVAEAYCIGKLKRSLGLMGCGSTSLSEEFFHQTMQLSGQIPSHIMSVLDMGQGILHNTSDLYGLITEQFLCYALDLLYDVIPVSYTSSDDQITLVKTPSLDIEGGSDAAEWLEMICFHEFLSSKLNKFVSPKSVIGTFVAEFKSRFFVMGEETPLLTKFVSAALHNVKCKTPTQLSETIDTICDQCIANGVSTKIVARISKRVNQLIRYSGYGDTPFGAIEDQDVKDWVDGSRGYRLQRKIEAIFYDDKETSFIRNCARKVFNDIKRGRIFEENLINLIGRGGDEALTGFLQYAGCSEQEVNRVLNYRWVNLSSFGDLRLVLRTKLMTSRRVLEREEVPTLIKTLQSKLSRNFTKGVKKILAESINKSAFQSSVASGFIGFCKSMGSKCVRDGKGGFLYIKEVYSGINVCICEICALKPKIIYCNDSLNKVSQFSKPILWDYFSLVLTNACELGEWVFSTVKEPQKPLVLNNQNFFWAVKPKVVRQIEDQLGMNHVLQSIRRNYPVLFDEHLAPFMNDLQVSRTMDSGRLKFLDVCIALDMMNENLGIISHLLKTRDNSVYIVKQSDCALAHIRQSSYTDWELGLSPQQICTNFKTQLVLSSMVNPLVLSTSCLKSFFWFNEVLELEDDSQIELAELTDFALMVKNQNVSRAMFVEDIAMGYVVSNFEGVRISLSNVMVDGVQLPPKEKAPDVGVLFGLKAENVIVGLVVQIDHVRMSTKFKLRRKMVYSFSLECTMDVGDIQNKEVILKVVAVDQSVSGSGGNHMLLDGVPVIASLPLFTGQASFDLAAMLIESNLAGSNDNFLMSNVTLDLGGFSPELSDKYSYRLSGPENQEDPLVLKDGAFYVGGERLSTYKVELTGDLVVKALGALEDDEGVVSMLHQLWPYLKATSQVILFQQEDFTIVHDLYKIQLTKSIESFGEWIEFTNFKVAYSKSLKELVISDTQGSFRLKGVMCRPLANTLQVEDIE if4e_mouse Eukaryotic translation initiation factor 4E Eif4e eIF-4F 25 kDa subunit protein protein mouse Mus musculus Mouse MATVEPETTPTTNPPPAEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENRDAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV q8az57_machu Pre-glycoprotein polyprotein GP complex GPC protein protein machu machupo virus MACV MGQLISFFQEIPVFLQEALNIALVAVSLIAVIKGIINLYKSGLFQFIFFLLLAGRSCSDGTFKIGLHTEFQSVTLTMQRLLANHSNELPSLCMLNNSFYYMRGGVNTFLIRVSDISVLMKEYDVSIYEPEDLGNCLNKSDSSWAIHWFSNALGHDWLMDPPMLCRNKTKKEGSNIQFNISKADDARVYGKKIRNGMRHLFRGFHDPCEEGKVCYLTINQCGDPSSFDYCGVNHLSKCQFDHVNTLHFLVRSKTHLNFERSLKAFFSWSLTDSSGKDMPGGYCLEEWMLIAAKMKCFGNTAVAKCNQNHDSEFCDMLRLFDYNKNAIKTLNDESKKEINLLSQTVNALISDNLLMKNKIKELMSIPYCNYTKFWYVNHTLTGQHTLPRCWLIRNGSYLNTSEFRNDWILESDHLISEMLSKEYAERQGKTPITLVDICFWSTIFFTASLFLHLVGIPTHRHLKGEACPLPHKLDSFGGCRCGKYPRLKKPTIWHKRH tfr1_human Transferrin receptor protein 1 TFRC T9 p90 protein protein human Homo sapiens Human MMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAVDEEENADNNTKANVTKPKRCSGSICYGTIAVIVFFLIGFMIGYLGYCKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF z-l-1 3233079 N/A 3233084 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3233085 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false l-z-1 3233081 N/A 3233084 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3233085 unspecified role unspecified role unspecified role unspecified role MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false l-z-2 3233082 N/A 3233084 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3233085 unspecified role unspecified role unspecified role unspecified role GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false z-eif4e-4 3233083 N/A 3233087 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3233085 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false gpc-tfrc-1 3205500 N/A 3205502 unspecified role unspecified role unspecified role unspecified role Region 79-258 Region 79-258 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3205503 unspecified role unspecified role unspecified role unspecified role Ectodomain Region 117-760 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction false false false UniProt UniProt http://www.uniprot.org fan-2010-1 Z proteins of New World arenaviruses bind RIG-I and interfere with type I interferon induction. human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fan L., Briese T., Lipkin WI. wil2001@columbia.edu J. Virol. (0022-538X) 2010 Only protein-protein interactions imex curation fan-2010-4 Z proteins of New World arenaviruses bind RIG-I and interfere with type I interferon induction. in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Fan L., Briese T., Lipkin WI. wil2001@columbia.edu J. Virol. (0022-538X) 2010 Only protein-protein interactions imex curation z_sabvb RING finger protein Z Z Zinc-binding protein protein protein sabvbm Sabia mammarenavirus (isolate Human/Brasil/SPH114202/1990) MGNSKSKSKLSANQYEQQTVNSTKQVAILKRQAEPSLYGRHNCRCCWFANTNLIKCSDHYICLKCLNIMLGKSSFCDICGEELPTSIVVPIEPSAPPPED z_machu RING finger protein Z Z Zinc-binding protein protein protein machu machupo virus MACV MGNCNKPPKRPPNTQTSAAQPSAEFRRTALPSLYGRYNCKCCWFADTNLITCNDHYLCLRCHQTMLRNSELCHICWKPLPTSITVPVEPSAPPP z_gtovv RING finger protein Z Z Zinc-binding protein protein protein gtovv guanarito virus (iso MGNSKSKSNPSSSSESQKGAPTVTEFRRTAIHSLYGRYNCKCCWFADKNLIKCSDHYLCLRCLNVMLKNSDLCNICWEQLPTCITVPEEPSAPPE ddx58_human Antiviral innate immune response receptor RIG-I DEAD box protein 58 Retinoic acid-inducible gene 1 protein DDX58 Probable ATP-dependent RNA helicase DDX58 Retinoic acid-inducible gene I protein RIG-I-like receptor 1 protein protein human Homo sapiens Human MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK z_junin RING finger protein Z Z Zinc-binding protein protein protein juninm Junin mammarenavirus MGNCNGASKSNQPDSSRVTQPAAEFRRVAHSSLYGRYNCKCCWFADTNLITCNDHYLCLRCHQVMLRNSDLCNICWKPLPTTITVPVEPTAPPP ddx58-z-jun-1 1021517 n/a 1021521 unspecified role unspecified role prey prey n/a 1021522 unspecified role unspecified role bait bait n-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 2A ddx58-z-jun-2 1021517 n/a 1021521 unspecified role unspecified role prey prey n/a 1021522 unspecified role unspecified role bait bait c-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 2A ddx58-z-gtov-1 1021517 n/a 1021522 unspecified role unspecified role bait bait n-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1021523 unspecified role unspecified role prey prey physical association physical association false false false fig. 2a ddx58-z-gtov-2 1021517 n/a 1021523 unspecified role unspecified role prey prey n/a 1021522 unspecified role unspecified role bait bait c-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 2A ddx58-z-macv-1 1021517 n/a 1021522 unspecified role unspecified role bait bait n-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1021524 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2A ddx58-z-macv-2 1021517 n/a 1021524 unspecified role unspecified role prey prey n/a 1021522 unspecified role unspecified role bait bait c-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 2A ddx58-z-sabv-1 1021517 n/a 1021525 unspecified role unspecified role prey prey n/a 1021522 unspecified role unspecified role bait bait n-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 2A ddx58-z-sabv-2 1021517 n/a 1021522 unspecified role unspecified role unspecified role unspecified role c-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false n/a 1021525 unspecified role unspecified role unspecified role unspecified role physical association physical association false false false Fig. 2A z-gtov-ddx58 1021518 n/a 1021523 unspecified role unspecified role bait bait n-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1021522 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Fig. 3A z-junv-ddx58 1021518 n/a 1021521 unspecified role unspecified role bait bait n-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1021522 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Fig. 3A z-ddx58 1021518 n/a 1021522 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n/a 1021524 unspecified role unspecified role bait bait n-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false direct interaction direct interaction false false false Fig. 3A