DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ fan-2005-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Fan H., Ooi A., Tan YW., Wang S., Fang S., Liu DX., Lescar J. Coronavirus - Interactions investigated in the context of Coronavirus DIP original experiment: DIP-132878X Structure (London, England : 1993) 2005 IMEx The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. Fan H., Ooi A., Tan YW., Wang S., Fang S., Liu DX., Lescar J. Only protein-protein interactions imex curation fan-2005-3 in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Fan H., Ooi A., Tan YW., Wang S., Fang S., Liu DX., Lescar J. Coronavirus - Interactions investigated in the context of Coronavirus DIP original experiment: DIP-132879X Structure (London, England : 1993) 2005 IMEx The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. Fan H., Ooi A., Tan YW., Wang S., Fang S., Liu DX., Lescar J. Only protein-protein interactions imex curation fan-2005-4 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Fan H., Ooi A., Tan YW., Wang S., Fang S., Liu DX., Lescar J. Coronavirus - Interactions investigated in the context of Coronavirus DIP original experiment: DIP-132880X Structure (London, England : 1993) 2005 IMEx The nucleocapsid protein of coronavirus infectious bronchitis virus: crystal structure of its N-terminal domain and multimerization properties. Fan H., Ooi A., Tan YW., Wang S., Fang S., Liu DX., Lescar J. Only protein-protein interactions imex curation jayaram-2006-1 ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Jayaram H., Fan H., Bowman BR., Ooi A., Jayaram J., Collisson EW., Lescar J., Prasad BV. Virus - Publications including interactions involving viral proteins julien@ntu.edu.sg Journal of virology 2006 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus ncap_ibvb Nucleoprotein N Nucleocapsid protein 6 protein protein ibvb ibvb IBV MASGKAAGKTDAPAPVIKLGGPKPPKVGSSGNASWFQAIKAKKLNTPPPKFEGSGVPDNENIKPSQQHGYWRRQARFKPGKGGRKPVPDAWYFYYTGTGPAADLNWGDTQDGIVWVAAKGADTKSRSNQGTRDPDKFDQYPLRFSDGGPDGNFRWDFIPLNRGRSGRSTAASSAAASRAPSREGSRGRRSDSGDDLIARAAKIIQDQQKKGSRITKAKADEMAHRRYCKRTIPPNYRVDQVFGPRTKGKEGNFGDDKMNEEGIKDGRVTAMLNLVPSSHACLFGSRVTPKLQLDGLHLRFEFTTVVPCDDPQFDNYVKICDQCVDGVGTRPKDDEPKPKSRSSSRPATRGNSPAPRQQRPKKEKKLKKQDDEADKALTSDEERNNAQLEFYDEPKVINWGDAALGENEL 562F39C475DB8872 ncap_ibvg Nucleoprotein N Nucleocapsid protein 6 protein protein IBV Avian infectious bronchitis virus (strain Gray) MASGKATGKTDAPAPVIKLGGPRPPKVGSSGNASWFQAIKAKKLNSPQPKFEGSGVPDNENFKTSQQHGYWRRQARFKPGKGRRKPVPDAWYFYYTGTGPAADLNWGDSQDGIVWVAAKGADVKSRSNQGTRDPDKFDQYPLRFSDGGPDGNFRWDFIPLNRGRSGRSTAASSAASSRPPSREGSRGRRSGSEDDLIARAAKIIQDQQKKGSRITKAKADEMAHRRYCKRTIPPGYKVDQVFGPRTKGKEGNFGDDKMNEEGIKDGRVTAMLNLVPSSHACLFGSRVTPKLQPDGLHLKFEFTTVVPRDDPQFDNYVKICDQCVDGVGTRPKDDEPKPKSRSSSRPATRTSSPAPRQQRLKKEKRPKKQDDEVDKALTSDEERNNAQLEFDDEPKVINWGDSALGENEL ibvn-1 3139899 N/A 3139902 unspecified role unspecified role unspecified role unspecified role N-terminal Region 29-160 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false C-terminal Region 218-329 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ibvn-2 3139900 N/A 3139902 unspecified role unspecified role unspecified role unspecified role N-terminal Region 29-160 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false C-terminal Region 218-329 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ibvn-3 3139901 N/A 3139902 unspecified role unspecified role unspecified role unspecified role N-terminal Region 29-160 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false n-58 3716302 N/A 3716303 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified P32923:p.Phe62Leu mutation mutation certain certain sequence position certain certain certain sequence position certain false 19-162 binding region 19-162 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 1b, Tab 1, Tab 2 n-59 3716302 N/A 3139902 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified 219-349 binding region 219-349 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 2, Tab 1, Tab 2 n-60 3716302 N/A 3716303 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified 219-349 binding region 219-349 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Tab 1, Tab 2 IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ tan-2012-2 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Tan YW., Hong W., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@ntu.edu.sg Nucleic acids research 2012 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus xiao-2007-9 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Xiao H., Xu LH., Yamada Y., Liu DX. Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg PloS one 2007 Only protein-protein interactions imex curation imex curation xiao-2007-10 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Xiao H., Xu LH., Yamada Y., Liu DX. Coronavirus - Interactions investigated in the context of Coronavirus dxliu@imcb.a-star.edu.sg PloS one 2007 Only protein-protein interactions imex curation imex curation xiao-2007-13 chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xiao H., Xu LH., Yamada Y., Liu DX. Coronavirus - Interactions investigated in the context of Coronavirus IBV-infected Vero cells dxliu@imcb.a-star.edu.sg PloS one 2007 Only protein-protein interactions imex curation imex curation wang-2015-88 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as tag visualisation tag visualisation Wang Y., Sun Y., Wu A., Xu S., Pan R., Zeng C., Jin X., Ge X., Shi Z., Ahola T., Chen Y., Guo D. Virus - Publications including interactions involving viral proteins nsp10 and nsp16 of the various coronaviruses were incubated with the substrate m7GpppA-RNA in the presence of [3H]AdoMet. Samples were then analyzed by liquid scintillation assays after purification of the 3H-labeled substrates with DEAE-Sephadex chromatography. Uniprot identifiers entered here are based on reviewed entries with the highest sequence identities to the stated Genbank accession numbers. chenyu@whu.edu.cn,dguo@whu.edu.cn Journal of virology 2015 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus wang-2015-90 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as predetermined predetermined participant predetermined Wang Y., Sun Y., Wu A., Xu S., Pan R., Zeng C., Jin X., Ge X., Shi Z., Ahola T., Chen Y., Guo D. Virus - Publications including interactions involving viral proteins Reaction mixtures were spotted onto TLC plates. Radiolabelling was determined using a PhosphorImager. (see ref. 28 pmid:22022266). Uniprot identifiers entered here are based on reviewed entries with the highest sequence identities to the stated Genbank accession numbers. chenyu@whu.edu.cn,dguo@whu.edu.cn Journal of virology 2015 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus xu-2010-22 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Journal of virology 2010 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus xu-2010-21 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg HeLa cell Journal of virology 2010 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus xu-2010-23 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Journal of virology 2010 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus xu-2010-25 chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Journal of virology 2010 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus xu-2010-26 chlae-vero Chlorocebus (Cercopithecus) aethiops kidney cells Grivet vero Chlorocebus (Cercopithecus) aethiops kidney cells similar to cos-7 cells but these are not trasnformed confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Xu L., Khadijah S., Fang S., Wang L., Tay FP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Journal of virology 2010 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus chen-2009-48 in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Chen B., Fang S., Tam JP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Virology 2009 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus chen-2009-45 in vitro In vitro crosslink cross-linking study crosslink weight by staining molecular weight estimation by staining weight by staining Chen B., Fang S., Tam JP., Liu DX. Virus - Publications including interactions involving viral proteins Cross-linking agent: Glutaraldehyde (CHEBI:64276) dxliu@imcb.a-star.edu.sg Virology 2009 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus chen-2009-46 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chen B., Fang S., Tam JP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Virology 2009 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus chen-2009-47 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chen B., Fang S., Tam JP., Liu DX. Virus - Publications including interactions involving viral proteins dxliu@imcb.a-star.edu.sg Virology 2009 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus q98vg9-pro_5000140223 Putative 2'-O-methyl transferase rep ORF1ab polyprotein 1a-1b protein protein FCoV Feline coronavirus (strain FIPV WSU-79/1146) SAEWNPGYSMPTLYKIQRMCLERCNLYNYGAQVRLPDGITTNVVKYTQLCQYLNTTTVCVPHKMRVLHLGAAGASGVAPGSTVLRRWLPDDAILVDNDLRDYVSDADFSVTGDCTSLYIEDKFDLLISDLYDGSTKSIDGENTSKDGFFTYINGFIKEKLSLGGSAAIKITEFSWNKDLYELIQRFEYWTVFCTSVNTSSSEGFLIGINYLGPYCDKAIVDGNIMHANYIFWRNSTIMALSHNSVLDTPKFKCRCNNALIVNLKEKELNEMVVGLLRKGKLLIRNNGKLLNFGNHLVNVP 6410 6709 q98vg9-pro_5000140217 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein FCoV Feline coronavirus (strain FIPV WSU-79/1146) AGKPTEHPSNSGLLTLCAFAPDPAKAYVDAVKRGMQPVTNCVKMLSNGAGNGMAITNGVESNTQQDSYGGASVCIYCRCHVEHPAIDGLCRFKGKFVQVPTGTQDPIRFCIENEVCVVCGCWLTNGCMCDRTSIQ 3889 4023 p0c6y0-pro_0000037368 2'-O-methyl transferase rep ORF1ab polyprotein 1a-1b protein protein MHV-JHM Murine coronavirus (strain JHM) Murine hepatitis virus AAADWKPGYVMPVLYKYLESPMERVNLWNYGKPITLPTGCMMNVAKYTQLCQYLSTTTLAVPANMRVLHLGAGSDKGVAPGSAVLRQWLPSGSILVDNDMNPFVSDSVASYYGNCITLPFDCQWDLIISDMYDPLTKNIGEYNVSKDGFFTYLCHLIRDKLALGGSVAIKITEFSWNAELYSLMGKFAFWTIFCTNVNASSSEGFLIGINWLNRTRNEIDGKTMHANYLFWRNSTMWNGGAYSLFDMTKFPLKAAGTAVVSLKPDQINDLVLSLIEKGKLLVRDTRKEVFVGDSLVNVK 6882 7180 p0c6y0-pro_0000037363 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein MHV-JHM Murine coronavirus (strain JHM) Murine hepatitis virus AGTATEYASNSAILSLCAFSVDPKKTYLDYIQQGGVPVTNCVKMLCDHAGTGMAITIKPEATTNQDSYGGASVCIYCRSRVEHPDVDGLCKLRGKFVQVPLGIKDPVSYVLTHDVCQVCGFWRDGSCSCVGTGSQFQ 4324 4460 p0c6x7-pro_0000037322 2'-O-methyltransferase nsp16 ORF1ab polyprotein 1a-1b rep protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus ASQAWQPGVAMPNLYKMQRMLLEKCDLQNYGENAVIPKGIMMNVAKYTQLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDSDLNDFVSDADSTLIGDCATVHTANKWDLIISDMYDPRTKHVTKENDSKEGFFTYLCGFIKQKLALGGSIAVKITEHSWNADLYKLMGHFSWWTAFVTNVNASSSEAFLIGANYLGKPKEQIDGYTMHANYIFWRNTNPIQLSSYSLFDMSKFPLKLRGTAVMSLKENQINDMIYSLLEKGRLIIRENNRVVVSSDILVNN 6776 7073 p0c6x7-pro_0000037317 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus AGNATEVPANSTVLSFCAFAVDPAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLRNTVCTVCGMWKGYGCSCDQLREPLMQ 4231 4369 k9n7c7-pro_0000422453 2'-O-methyltransferase rep ORF1ab polyprotein 1a-1b protein protein cvemc Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1) Betacoronavirus England 1 Human coronavirus EMC ASADWKPGHAMPSLFKVQNVNLERCELANYKQSIPMPRGVHMNIAKYMQLCQYLNTCTLAVPANMRVIHFGAGSDKGIAPGTSVLRQWLPTDAIIIDNDLNEFVSDADITLFGDCVTVRVGQQVDLVISDMYDPTTKNVTGSNESKALFFTYLCNLINNNLALGGSVAIKITEHSWSVELYELMGKFAWWTVFCTNANASSSEGFLLGINYLGTIKENIDGGAMHANYIFWRNSTPMNLSTYSLFDLSKFQLKLKGTPVLQLKESQINELVISLLSQGKLLIRDNDTLSVSTDVLVNTYRKLR 6776 7078 k9n7c7-pro_0000422448 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein cvemc Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (Betacoronavirus England 1) Betacoronavirus England 1 Human coronavirus EMC AGSNTEFASNSSVLSLVNFTVDPQKAYLDFVNAGGAPLTNCVKMLTPKTGTGIAISVKPESTADQETYGGASVCLYCRAHIEHPDVSGVCKYKGKFVQIPAQCVRDPVGFCLSNTPCNVCQYWIGYGCNCDSLRQAALPQ 4238 4377 pppac20-rna-substrate pppAC20 RNA substrate rna ribonucleic acid RNA rna synthetic construct synthetic construct ACCCCCCCCCCCCCCCCCCCC mce_vaccw Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase PAPS Poly(A) polymerase regulatory subunit Poly(A) polymerase small subunit VP39 J3R VACWR095 protein protein vaccw vaccinia virus (stra Vaccinia virus (strain WR) VACV MDVVSLDKPFMYFEEIDNELDYEPESANEVAKKLPYQGQLKLLLGELFFLSKLQRHGILDGATVVYIGSAPGTHIRYLRDHFYNLGVIIKWMLIDGRHHDPILNGLRDVTLVTRFVDEEYLRSIKKQLHPSKIILISDVRSKRGGNEPSTADLLSNYALQNVMISILNPVASSLKWRCPFPDQWIKDFYIPHGNKMLQPFAPSYSAEMRLLSIYTGENMRLTRVTKSDAVNYEKKMYYLNKIVRNKVVVNFDYPNQEYDYFHMYFMLRTVYCNKTFPTTKAKVLFLQQSIFRFLNIPTTSTEKVSHEPIQRKISSKNSMSKNRNSKRSVRSNK 55A299DA4AAE1AD4 p0c6y5-pro_0000037400 Putative 2'-O-methyl transferase rep ORF1ab polyprotein 1a-1b protein protein TGEV Porcine transmissible gastroenteritis coronavirus (strain Purdue) SAEWNPGYSMPTLYKIQRMCLERCNLYNYGAQVKLPDGITTNVVKYTQLCQYLNTTTLCVPHKMRVLHLGAAGASGVAPGSTVLRRWLPDDAILVDNDLRDYVSDADFSVTGDCTSLYIEDKFDLLVSDLYDGSTKSIDGENTSKDGFFTYINGFIKEKLSLGGSVAIKITEFSWNKDLYELIQRFEYWTVFCTSVNTSSSEGFLIGINYLGPYCDKAIVDGNIMHANYIFWRNSTIMALSHNSVLDTPKFKCRCNNALIVNLKEKELNEMVIGLLRKGKLLIRNNGKLLNFGNHFVNTP 6385 6684 p0c6y5-pro_0000037395 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein TGEV Porcine transmissible gastroenteritis coronavirus (strain Purdue) AGKPTEHPSNSSLLTLCAFSPDPAKAYVDAVKRGMQPVNNCVKMLSNGAGNGMAVTNGVEANTQQDSYGGASVCIYCRCHVEHPAIDGLCRYKGKFVQIPTGTQDPIRFCIENEVCVVCGCWLNNGCMCDRTSMQ 3864 3998 p0c6y1-pro_0000037417 2'-O-methyl transferase rep ORF1ab polyprotein 1a-1b protein protein ibvb ibvb IBV SAWTCGYNMPELYKVQNCVMEPCNIPNYGVGITLPSGILMNVAKYTQLCQYLSKTTICVPHNMRVMHFGAGSDKGVAPGSTVLKQWLPEGTLLVDNDIVDYVSDAHVSVLSDCNKYNTEHKFDLVISDMYTDNDSKRKHEGVIANNGNDDVFIYLSSFLRNNLALGGSFAVKVTETSWHEVLYDIAQDCAWWTMFCTAVNASSSEAFLIGVNYLGASEKVKVSGKTLHANYIFWRNCNYLQTSAYSIFDVAKFDLRLKATPVVNLKTEQKTDLVFNLIKCGKLLVRDVGNTSFTSDSFVCTM 6328 6629 p0c6y1-pro_0000037412 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein ibvb ibvb IBV SKGHETEEVDAVGILSLCSFAVDPADTYCKYVAAGNQPLGNCVKMLTVHNGSGFAITSKPSPTPDQDSYGGASVCLYCRAHIAHPGSVGNLDGRCQFKGSFVQIPTTEKDPVGFCLRNKVCTVCQCWIGYGCQCDSLRQPKSSVQ 3784 3928 ssrna_sars-cov-1 Severe acute respiratory syndrome-related coronavirus 1 complete genome rna ribonucleic acid RNA rna sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus ATATTAGGTTTTTACCTACCCAGGAAAAGCCAACCAACCTCGATCTCTTGTAGATCTGTTCTCTAAACGAACTTTAAAATCTGTGTAGCTGTCGCTCGGCTGCATGCCTAGTGCACCTACGCAGTATAAACAATAATAAATTTTACTGTCGTTGACAAGAAACGAGTAACTCGTCCCTCTTCTGCAGACTGCTTACGGTTTCGTCCGTGTTGCAGTCGATCATCAGCATACCTAGGTTTCGTCCGGGTGTGACCGAAAGGTAAGATGGAGAGCCTTGTTCTTGGTGTCAACGAGAAAACACACGTCCAACTCAGTTTGCCTGTCCTTCAGGTTAGAGACGTGCTAGTGCGTGGCTTCGGGGACTCTGTGGAAGAGGCCCTATCGGAGGCACGTGAACACCTCAAAAATGGCACTTGTGGTCTAGTAGAGCTGGAAAAAGGCGTACTGCCCCAGCTTGAACAGCCCTATGTGTTCATTAAACGTTCTGATGCCTTAAGCACCAATCACGGCCACAAGGTCGTTGAGCTGGTTGCAGAAATGGACGGCATTCAGTACGGTCGTAGCGGTATAACACTGGGAGTACTCGTGCCACATGTGGGCGAAACCCCAATTGCATACCGCAATGTTCTTCTTCGTAAGAACGGTAATAAGGGAGCCGGTGGTCATAGCTATGGCATCGATCTAAAGTCTTATGACTTAGGTGACGAGCTTGGCACTGATCCCATTGAAGATTATGAACAAAACTGGAACACTAAGCATGGCAGTGGTGCACTCCGTGAACTCACTCGTGAGCTCAATGGAGGTGCAGTCACTCGCTATGTCGACAACAATTTCTGTGGCCCAGATGGGTACCCTCTTGATTGCATCAAAGATTTTCTCGCACGCGCGGGCAAGTCAATGTGCACTCTTTCCGAACAACTTGATTACATCGAGTCGAAGAGAGGTGTCTACTGCTGCCGTGACCATGAGCATGAAATTGCCTGGTTCACTGAGCGCTCTGATAAGAGCTACGAGCACCAGACACCCTTCGAAATTAAGAGTGCCAAGAAATTTGACACTTTCAAAGGGGAATGCCCAAAGTTTGTGTTTCCTCTTAACTCAAAAGTCAAAGTCATTCAACCACGTGTTGAAAAGAAAAAGACTGAGGGTTTCATGGGGCGTATACGCTCTGTGTACCCTGTTGCATCTCCACAGGAGTGTAACAATATGCACTTGTCTACCTTGATGAAATGTAATCATTGCGATGAAGTTTCATGGCAGACGTGCGACTTTCTGAAAGCCACTTGTGAACATTGTGGCACTGAAAATTTAGTTATTGAAGGACCTACTACATGTGGGTACCTACCTACTAATGCTGTAGTGAAAATGCCATGTCCTGCCTGTCAAGACCCAGAGATTGGACCTGAGCATAGTGTTGCAGATTATCACAACCACTCAAACATTGAAACTCGACTCCGCAAGGGAGGTAGGACTAGATGTTTTGGAGGCTGTGTGTTTGCCTATGTTGGCTGCTATAATAAGCGTGCCTACTGGGTTCCTCGTGCTAGTGCTGATATTGGCTCAGGCCATACTGGCATTACTGGTGACAATGTGGAGACCTTGAATGAGGATCTCCTTGAGATACTGAGTCGTGAACGTGTTAACATTAACATTGTTGGCGATTTTCATTTGAATGAAGAGGTTGCCATCATTTTGGCATCTTTCTCTGCTTCTACAAGTGCCTTTATTGACACTATAAAGAGTCTTGATTACAAGTCTTTCAAAACCATTGTTGAGTCCTGCGGTAACTATAAAGTTACCAAGGGAAAGCCCGTAAAAGGTGCTTGGAACATTGGACAACAGAGATCAGTTTTAACACCACTGTGTGGTTTTCCCTCACAGGCTGCTGGTGTTATCAGATCAATTTTTGCGCGCACACTTGATGCAGCAAACCACTCAATTCCTGATTTGCAAAGAGCAGCTGTCACCATACTTGATGGTATTTCTGAACAGTCATTACGTCTTGTCGACGCCATGGTTTATACTTCAGACCTGCTCACCAACAGTGTCATTATTATGGCATATGTAACTGGTGGTCTTGTACAACAGACTTCTCAGTGGTTGTCTAATCTTTTGGGCACTACTGTTGAAAAACTCAGGCCTATCTTTGAATGGATTGAGGCGAAACTTAGTGCAGGAGTTGAATTTCTCAAGGATGCTTGGGAGATTCTCAAATTTCTCATTACAGGTGTTTTTGACATCGTCAAGGGTCAAATACAGGTTGCTTCAGATAACATCAAGGATTGTGTAAAATGCTTCATTGATGTTGTTAACAAGGCACTCGAAATGTGCATTGATCAAGTCACTATCGCTGGCGCAAAGTTGCGATCACTCAACTTAGGTGAAGTCTTCATCGCTCAAAGCAAGGGACTTTACCGTCAGTGTATACGTGGCAAGGAGCAGCTGCAACTACTCATGCCTCTTAAGGCACCAAAAGAAGTAACCTTTCTTGAAGGTGATTCACATGACACAGTACTTACCTCTGAGGAGGTTGTTCTCAAGAACGGTGAACTCGAAGCACTCGAGACGCCCGTTGATAGCTTCACAAATGGAGCTATCGTTGGCACACCAGTCTGTGTAAATGGCCTCATGCTCTTAGAGATTAAGGACAAAGAACAATACTGCGCATTGTCTCCTGGTTTACTGGCTACAAACAATGTCTTTCGCTTAAAAGGGGGTGCACCAATTAAAGGTGTAACCTTTGGAGAAGATACTGTTTGGGAAGTTCAAGGTTACAAGAATGTGAGAATCACATTTGAGCTTGATGAACGTGTTGACAAAGTGCTTAATGAAAAGTGCTCTGTCTACACTGTTGAATCCGGTACCGAAGTTACTGAGTTTGCATGTGTTGTAGCAGAGGCTGTTGTGAAGACTTTACAACCAGTTTCTGATCTCCTTACCAACATGGGTATTGATCTTGATGAGTGGAGTGTAGCTACATTCTACTTATTTGATGATGCTGGTGAAGAAAACTTTTCATCACGTATGTATTGTTCCTTTTACCCTCCAGATGAGGAAGAAGAGGACGATGCAGAGTGTGAGGAAGAAGAAATTGATGAAACCTGTGAACATGAGTACGGTACAGAGGATGATTATCAAGGTCTCCCTCTGGAATTTGGTGCCTCAGCTGAAACAGTTCGAGTTGAGGAAGAAGAAGAGGAAGACTGGCTGGATGATACTACTGAGCAATCAGAGATTGAGCCAGAACCAGAACCTACACCTGAAGAACCAGTTAATCAGTTTACTGGTTATTTAAAACTTACTGACAATGTTGCCATTAAATGTGTTGACATCGTTAAGGAGGCACAAAGTGCTAATCCTATGGTGATTGTAAATGCTGCTAACATACACCTGAAACATGGTGGTGGTGTAGCAGGTGCACTCAACAAGGCAACCAATGGTGCCATGCAAAAGGAGAGTGATGATTACATTAAGCTAAATGGCCCTCTTACAGTAGGAGGGTCTTGTTTGCTTTCTGGACATAATCTTGCTAAGAAGTGTCTGCATGTTGTTGGACCTAACCTAAATGCAGGTGAGGACATCCAGCTTCTTAAGGCAGCATATGAAAATTTCAATTCACAGGACATCTTACTTGCACCATTGTTGTCAGCAGGCATATTTGGTGCTAAACCACTTCAGTCTTTACAAGTGTGCGTGCAGACGGTTCGTACACAGGTTTATATTGCAGTCAATGACAAAGCTCTTTATGAGCAGGTTGTCATGGATTATCTTGATAACCTGAAGCCTAGAGTGGAAGCACCTAAACAAGAGGAGCCACCAAACACAGAAGATTCCAAAACTGAGGAGAAATCTGTCGTACAGAAGCCTGTCGATGTGAAGCCAAAAATTAAGGCCTGCATTGATGAGGTTACCACAACACTGGAAGAAACTAAGTTTCTTACCAATAAGTTACTCTTGTTTGCTGATATCAATGGTAAGCTTTACCATGATTCTCAGAACATGCTTAGAGGTGAAGATATGTCTTTCCTTGAGAAGGATGCACCTTACATGGTAGGTGATGTTATCACTAGTGGTGATATCACTTGTGTTGTAATACCCTCCAAAAAGGCTGGTGGCACTACTGAGATGCTCTCAAGAGCTTTGAAGAAAGTGCCAGTTGATGAGTATATAACCACGTACCCTGGACAAGGATGTGCTGGTTATACACTTGAGGAAGCTAAGACTGCTCTTAAGAAATGCAAATCTGCATTTTATGTACTACCTTCAGAAGCACCTAATGCTAAGGAAGAGATTCTAGGAACTGTATCCTGGAATTTGAGAGAAATGCTTGCTCATGCTGAAGAGACAAGAAAATTAATGCCTATATGCATGGATGTTAGAGCCATAATGGCAACCATCCAACGTAAGTATAAAGGAATTAAAATTCAAGAGGGCATCGTTGACTATGGTGTCCGATTCTTCTTTTATACTAGTAAAGAGCCTGTAGCTTCTATTATTACGAAGCTGAACTCTCTAAATGAGCCGCTTGTCACAATGCCAATTGGTTATGTGACACATGGTTTTAATCTTGAAGAGGCTGCGCGCTGTATGCGTTCTCTTAAAGCTCCTGCCGTAGTGTCAGTATCATCACCAGATGCTGTTACTACATATAATGGATACCTCACTTCGTCATCAAAGACATCTGAGGAGCACTTTGTAGAAACAGTTTCTTTGGCTGGCTCTTACAGAGATTGGTCCTATTCAGGACAGCGTACAGAGTTAGGTGTTGAATTTCTTAAGCGTGGTGACAAAATTGTGTACCACACTCTGGAGAGCCCCGTCGAGTTTCATCTTGACGGTGAGGTTCTTTCACTTGACAAACTAAAGAGTCTCTTATCCCTGCGGGAGGTTAAGACTATAAAAGTGTTCACAACTGTGGACAACACTAATCTCCACACACAGCTTGTGGATATGTCTATGACATATGGACAGCAGTTTGGTCCAACATACTTGGATGGTGCTGATGTTACAAAAATTAAACCTCATGTAAATCATGAGGGTAAGACTTTCTTTGTACTACCTAGTGATGACACACTACGTAGTGAAGCTTTCGAGTACTACCATACTCTTGATGAGAGTTTTCTTGGTAGGTACATGTCTGCTTTAAACCACACAAAGAAATGGAAATTTCCTCAAGTTGGTGGTTTAACTTCAATTAAATGGGCTGATAACAATTGTTATTTGTCTAGTGTTTTATTAGCACTTCAACAGCTTGAAGTCAAATTCAATGCACCAGCACTTCAAGAGGCTTATTATAGAGCCCGTGCTGGTGATGCTGCTAACTTTTGTGCACTCATACTCGCTTACAGTAATAAAACTGTTGGCGAGCTTGGTGATGTCAGAGAAACTATGACCCATCTTCTACAGCATGCTAATTTGGAATCTGCAAAGCGAGTTCTTAATGTGGTGTGTAAACATTGTGGTCAGAAAACTACTACCTTAACGGGTGTAGAAGCTGTGATGTATATGGGTACTCTATCTTATGATAATCTTAAGACAGGTGTTTCCATTCCATGTGTGTGTGGTCGTGATGCTACACAATATCTAGTACAACAAGAGTCTTCTTTTGTTATGATGTCTGCACCACCTGCTGAGTATAAATTACAGCAAGGTACATTCTTATGTGCGAATGAGTACACTGGTAACTATCAGTGTGGTCATTACACTCATATAACTGCTAAGGAGACCCTCTATCGTATTGACGGAGCTCACCTTACAAAGATGTCAGAGTACAAAGGACCAGTGACTGATGTTTTCTACAAGGAAACATCTTACACTACAACCATCAAGCCTGTGTCGTATAAACTCGATGGAGTTACTTACACAGAGATTGAACCAAAATTGGATGGGTATTATAAAAAGGATAATGCTTACTATACAGAGCAGCCTATAGACCTTGTACCAACTCAACCATTACCAAATGCGAGTTTTGATAATTTCAAACTCACATGTTCTAACACAAAATTTGCTGATGATTTAAATCAAATGACAGGCTTCACAAAGCCAGCTTCACGAGAGCTATCTGTCACATTCTTCCCAGACTTGAATGGCGATGTAGTGGCTATTGACTATAGACACTATTCAGCGAGTTTCAAGAAAGGTGCTAAATTACTGCATAAGCCAATTGTTTGGCACATTAACCAGGCTACAACCAAGACAACGTTCAAACCAAACACTTGGTGTTTACGTTGTCTTTGGAGTACAAAGCCAGTAGATACTTCAAATTCATTTGAAGTTCTGGCAGTAGAAGACACACAAGGAATGGACAATCTTGCTTGTGAAAGTCAACAACCCACCTCTGAAGAAGTAGTGGAAAATCCTACCATACAGAAGGAAGTCATAGAGTGTGACGTGAAAACTACCGAAGTTGTAGGCAATGTCATACTTAAACCATCAGATGAAGGTGTTAAAGTAACACAAGAGTTAGGTCATGAGGATCTTATGGCTGCTTATGTGGAAAACACAAGCATTACCATTAAGAAACCTAATGAGCTTTCACTAGCCTTAGGTTTAAAAACAATTGCCACTCATGGTATTGCTGCAATTAATAGTGTTCCTTGGAGTAAAATTTTGGCTTATGTCAAACCATTCTTAGGACAAGCAGCAATTACAACATCAAATTGCGCTAAGAGATTAGCACAACGTGTGTTTAACAATTATATGCCTTATGTGTTTACATTATTGTTCCAATTGTGTACTTTTACTAAAAGTACCAATTCTAGAATTAGAGCTTCACTACCTACAACTATTGCTAAAAATAGTGTTAAGAGTGTTGCTAAATTATGTTTGGATGCCGGCATTAATTATGTGAAGTCACCCAAATTTTCTAAATTGTTCACAATCGCTATGTGGCTATTGTTGTTAAGTATTTGCTTAGGTTCTCTAATCTGTGTAACTGCTGCTTTTGGTGTACTCTTATCTAATTTTGGTGCTCCTTCTTATTGTAATGGCGTTAGAGAATTGTATCTTAATTCGTCTAACGTTACTACTATGGATTTCTGTGAAGGTTCTTTTCCTTGCAGCATTTGTTTAAGTGGATTAGACTCCCTTGATTCTTATCCAGCTCTTGAAACCATTCAGGTGACGATTTCATCGTACAAGCTAGACTTGACAATTTTAGGTCTGGCCGCTGAGTGGGTTTTGGCATATATGTTGTTCACAAAATTCTTTTATTTATTAGGTCTTTCAGCTATAATGCAGGTGTTCTTTGGCTATTTTGCTAGTCATTTCATCAGCAATTCTTGGCTCATGTGGTTTATCATTAGTATTGTACAAATGGCACCCGTTTCTGCAATGGTTAGGATGTACATCTTCTTTGCTTCTTTCTACTACATATGGAAGAGCTATGTTCATATCATGGATGGTTGCACCTCTTCGACTTGCATGATGTGCTATAAGCGCAATCGTGCCACACGCGTTGAGTGTACAACTATTGTTAATGGCATGAAGAGATCTTTCTATGTCTATGCAAATGGAGGCCGTGGCTTCTGCAAGACTCACAATTGGAATTGTCTCAATTGTGACACATTTTGCACTGGTAGTACATTCATTAGTGATGAAGTTGCTCGTGATTTGTCACTCCAGTTTAAAAGACCAATCAACCCTACTGACCAGTCATCGTATATTGTTGATAGTGTTGCTGTGAAAAATGGCGCGCTTCACCTCTACTTTGACAAGGCTGGTCAAAAGACCTATGAGAGACATCCGCTCTCCCATTTTGTCAATTTAGACAATTTGAGAGCTAACAACACTAAAGGTTCACTGCCTATTAATGTCATAGTTTTTGATGGCAAGTCCAAATGCGACGAGTCTGCTTCTAAGTCTGCTTCTGTGTACTACAGTCAGCTGATGTGCCAACCTATTCTGTTGCTTGACCAAGCTCTTGTATCAGACGTTGGAGATAGTACTGAAGTTTCCGTTAAGATGTTTGATGCTTATGTCGACACCTTTTCAGCAACTTTTAGTGTTCCTATGGAAAAACTTAAGGCACTTGTTGCTACAGCTCACAGCGAGTTAGCAAAGGGTGTAGCTTTAGATGGTGTCCTTTCTACATTCGTGTCAGCTGCCCGACAAGGTGTTGTTGATACCGATGTTGACACAAAGGATGTTATTGAATGTCTCAAACTTTCACATCACTCTGACTTAGAAGTGACAGGTGACAGTTGTAACAATTTCATGCTCACCTATAATAAGGTTGAAAACATGACGCCCAGAGATCTTGGCGCATGTATTGACTGTAATGCAAGGCATATCAATGCCCAAGTAGCAAAAAGTCACAATGTTTCACTCATCTGGAATGTAAAAGACTACATGTCTTTATCTGAACAGCTGCGTAAACAAATTCGTAGTGCTGCCAAGAAGAACAACATACCTTTTAGACTAACTTGTGCTACAACTAGACAGGTTGTCAATGTCATAACTACTAAAATCTCACTCAAGGGTGGTAAGATTGTTAGTACTTGTTTTAAACTTATGCTTAAGGCCACATTATTGTGCGTTCTTGCTGCATTGGTTTGTTATATCGTTATGCCAGTACATACATTGTCAATCCATGATGGTTACACAAATGAAATCATTGGTTACAAAGCCATTCAGGATGGTGTCACTCGTGACATCATTTCTACTGATGATTGTTTTGCAAATAAACATGCTGGTTTTGACGCATGGTTTAGCCAGCGTGGTGGTTCATACAAAAATGACAAAAGCTGCCCTGTAGTAGCTGCTATCATTACAAGAGAGATTGGTTTCATAGTGCCTGGCTTACCGGGTACTGTGCTGAGAGCAATCAATGGTGACTTCTTGCATTTTCTACCTCGTGTTTTTAGTGCTGTTGGCAACATTTGCTACACACCTTCCAAACTCATTGAGTATAGTGATTTTGCTACCTCTGCTTGCGTTCTTGCTGCTGAGTGTACAATTTTTAAGGATGCTATGGGCAAACCTGTGCCATATTGTTATGACACTAATTTGCTAGAGGGTTCTATTTCTTATAGTGAGCTTCGTCCAGACACTCGTTATGTGCTTATGGATGGTTCCATCATACAGTTTCCTAACACTTACCTGGAGGGTTCTGTTAGAGTAGTAACAACTTTTGATGCTGAGTACTGTAGACATGGTACATGCGAAAGGTCAGAAGTAGGTATTTGCCTATCTACCAGTGGTAGATGGGTTCTTAATAATGAGCATTACAGAGCTCTATCAGGAGTTTTCTGTGGTGTTGATGCGATGAATCTCATAGCTAACATCTTTACTCCTCTTGTGCAACCTGTGGGTGCTTTAGATGTGTCTGCTTCAGTAGTGGCTGGTGGTATTATTGCCATATTGGTGACTTGTGCTGCCTACTACTTTATGAAATTCAGACGTGTTTTTGGTGAGTACAACCATGTTGTTGCTGCTAATGCACTTTTGTTTTTGATGTCTTTCACTATACTCTGTCTGGTACCAGCTTACAGCTTTCTGCCGGGAGTCTACTCAGTCTTTTACTTGTACTTGACATTCTATTTCACCAATGATGTTTCATTCTTGGCTCACCTTCAATGGTTTGCCATGTTTTCTCCTATTGTGCCTTTTTGGATAACAGCAATCTATGTATTCTGTATTTCTCTGAAGCACTGCCATTGGTTCTTTAACAACTATCTTAGGAAAAGAGTCATGTTTAATGGAGTTACATTTAGTACCTTCGAGGAGGCTGCTTTGTGTACCTTTTTGCTCAACAAGGAAATGTACCTAAAATTGCGTAGCGAGACACTGTTGCCACTTACACAGTATAACAGGTATCTTGCTCTATATAACAAGTACAAGTATTTCAGTGGAGCCTTAGATACTACCAGCTATCGTGAAGCAGCTTGCTGCCACTTAGCAAAGGCTCTAAATGACTTTAGCAACTCAGGTGCTGATGTTCTCTACCAACCACCACAGACATCAATCACTTCTGCTGTTCTGCAGAGTGGTTTTAGGAAAATGGCATTCCCGTCAGGCAAAGTTGAAGGGTGCATGGTACAAGTAACCTGTGGAACTACAACTCTTAATGGATTGTGGTTGGATGACACAGTATACTGTCCAAGACATGTCATTTGCACAGCAGAAGACATGCTTAATCCTAACTATGAAGATCTGCTCATTCGCAAATCCAACCATAGCTTTCTTGTTCAGGCTGGCAATGTTCAACTTCGTGTTATTGGCCATTCTATGCAAAATTGTCTGCTTAGGCTTAAAGTTGATACTTCTAACCCTAAGACACCCAAGTATAAATTTGTCCGTATCCAACCTGGTCAAACATTTTCAGTTCTAGCATGCTACAATGGTTCACCATCTGGTGTTTATCAGTGTGCCATGAGACCTAATCATACCATTAAAGGTTCTTTCCTTAATGGATCATGTGGTAGTGTTGGTTTTAACATTGATTATGATTGCGTGTCTTTCTGCTATATGCATCATATGGAGCTTCCAACAGGAGTACACGCTGGTACTGACTTAGAAGGTAAATTCTATGGTCCATTTGTTGACAGACAAACTGCACAGGCTGCAGGTACAGACACAACCATAACATTAAATGTTTTGGCATGGCTGTATGCTGCTGTTATCAATGGTGATAGGTGGTTTCTTAATAGATTCACCACTACTTTGAATGACTTTAACCTTGTGGCAATGAAGTACAACTATGAACCTTTGACACAAGATCATGTTGACATATTGGGACCTCTTTCTGCTCAAACAGGAATTGCCGTCTTAGATATGTGTGCTGCTTTGAAAGAGCTGCTGCAGAATGGTATGAATGGTCGTACTATCCTTGGTAGCACTATTTTAGAAGATGAGTTTACACCATTTGATGTTGTTAGACAATGCTCTGGTGTTACCTTCCAAGGTAAGTTCAAGAAAATTGTTAAGGGCACTCATCATTGGATGCTTTTAACTTTCTTGACATCACTATTGATTCTTGTTCAAAGTACACAGTGGTCACTGTTTTTCTTTGTTTACGAGAATGCTTTCTTGCCATTTACTCTTGGTATTATGGCAATTGCTGCATGTGCTATGCTGCTTGTTAAGCATAAGCACGCATTCTTGTGCTTGTTTCTGTTACCTTCTCTTGCAACAGTTGCTTACTTTAATATGGTCTACATGCCTGCTAGCTGGGTGATGCGTATCATGACATGGCTTGAATTGGCTGACACTAGCTTGTCTGGTTATAGGCTTAAGGATTGTGTTATGTATGCTTCAGCTTTAGTTTTGCTTATTCTCATGACAGCTCGCACTGTTTATGATGATGCTGCTAGACGTGTTTGGACACTGATGAATGTCATTACACTTGTTTACAAAGTCTACTATGGTAATGCTTTAGATCAAGCTATTTCCATGTGGGCCTTAGTTATTTCTGTAACCTCTAACTATTCTGGTGTCGTTACGACTATCATGTTTTTAGCTAGAGCTATAGTGTTTGTGTGTGTTGAGTATTACCCATTGTTATTTATTACTGGCAACACCTTACAGTGTATCATGCTTGTTTATTGTTTCTTAGGCTATTGTTGCTGCTGCTACTTTGGCCTTTTCTGTTTACTCAACCGTTACTTCAGGCTTACTCTTGGTGTTTATGACTACTTGGTCTCTACACAAGAATTTAGGTATATGAACTCCCAGGGGCTTTTGCCTCCTAAGAGTAGTATTGATGCTTTCAAGCTTAACATTAAGTTGTTGGGTATTGGAGGTAAACCATGTATCAAGGTTGCTACTGTACAGTCTAAAATGTCTGACGTAAAGTGCACATCTGTGGTACTGCTCTCGGTTCTTCAACAACTTAGAGTAGAGTCATCTTCTAAATTGTGGGCACAATGTGTACAACTCCACAATGATATTCTTCTTGCAAAAGACACAACTGAAGCTTTCGAGAAGATGGTTTCTCTTTTGTCTGTTTTGCTATCCATGCAGGGTGCTGTAGACATTAATAGGTTGTGCGAGGAAATGCTCGATAACCGTGCTACTCTTCAGGCTATTGCTTCAGAATTTAGTTCTTTACCATCATATGCCGCTTATGCCACTGCCCAGGAGGCCTATGAGCAGGCTGTAGCTAATGGTGATTCTGAAGTCGTTCTCAAAAAGTTAAAGAAATCTTTGAATGTGGCTAAATCTGAGTTTGACCGTGATGCTGCCATGCAACGCAAGTTGGAAAAGATGGCAGATCAGGCTATGACCCAAATGTACAAACAGGCAAGATCTGAGGACAAGAGGGCAAAAGTAACTAGTGCTATGCAAACAATGCTCTTCACTATGCTTAGGAAGCTTGATAATGATGCACTTAACAACATTATCAACAATGCGCGTGATGGTTGTGTTCCACTCAACATCATACCATTGACTACAGCAGCCAAACTCATGGTTGTTGTCCCTGATTATGGTACCTACAAGAACACTTGTGATGGTAACACCTTTACATATGCATCTGCACTCTGGGAAATCCAGCAAGTTGTTGATGCGGATAGCAAGATTGTTCAACTTAGTGAAATTAACATGGACAATTCACCAAATTTGGCTTGGCCTCTTATTGTTACAGCTCTAAGAGCCAACTCAGCTGTTAAACTACAGAATAATGAACTGAGTCCAGTAGCACTACGACAGATGTCCTGTGCGGCTGGTACCACACAAACAGCTTGTACTGATGACAATGCACTTGCCTACTATAACAATTCGAAGGGAGGTAGGTTTGTGCTGGCATTACTATCAGACCACCAAGATCTCAAATGGGCTAGATTCCCTAAGAGTGATGGTACAGGTACAATTTACACAGAACTGGAACCACCTTGTAGGTTTGTTACAGACACACCAAAAGGGCCTAAAGTGAAATACTTGTACTTCATCAAAGGCTTAAACAACCTAAATAGAGGTATGGTGCTGGGCAGTTTAGCTGCTACAGTACGTCTTCAGGCTGGAAATGCTACAGAAGTACCTGCCAATTCAACTGTGCTTTCCTTCTGTGCTTTTGCAGTAGACCCTGCTAAAGCATATAAGGATTACCTAGCAAGTGGAGGACAACCAATCACCAACTGTGTGAAGATGTTGTGTACACACACTGGTACAGGACAGGCAATTACTGTAACACCAGAAGCTAACATGGACCAAGAGTCCTTTGGTGGTGCTTCATGTTGTCTGTATTGTAGATGCCACATTGACCATCCAAATCCTAAAGGATTCTGTGACTTGAAAGGTAAGTACGTCCAAATACCTACCACTTGTGCTAATGACCCAGTGGGTTTTACACTTAGAAACACAGTCTGTACCGTCTGCGGAATGTGGAAAGGTTATGGCTGTAGTTGTGACCAACTCCGCGAACCCTTGATGCAGTCTGCGGATGCATCAACGTTTTTAAACGGGTTTGCGGTGTAAGTGCAGCCCGTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTCTACAGGGCTTTTGATATTTACAACGAAAAAGTTGCTGGTTTTGCAAAGTTCCTAAAAACTAATTGCTGTCGCTTCCAGGAGAAGGATGAGGAAGGCAATTTATTAGACTCTTACTTTGTAGTTAAGAGGCATACTATGTCTAACTACCAACATGAAGAGACTATTTATAACTTGGTTAAAGATTGTCCAGCGGTTGCTGTCCATGACTTTTTCAAGTTTAGAGTAGATGGTGACATGGTACCACATATATCACGTCAGCGTCTAACTAAATACACAATGGCTGATTTAGTCTATGCTCTACGTCATTTTGATGAGGGTAATTGTGATACATTAAAAGAAATACTCGTCACATACAATTGCTGTGATGATGATTATTTCAATAAGAAGGATTGGTATGACTTCGTAGAGAATCCTGACATCTTACGCGTATATGCTAACTTAGGTGAGCGTGTACGCCAATCATTATTAAAGACTGTACAATTCTGCGATGCTATGCGTGATGCAGGCATTGTAGGCGTACTGACATTAGATAATCAGGATCTTAATGGGAACTGGTACGATTTCGGTGATTTCGTACAAGTAGCACCAGGCTGCGGAGTTCCTATTGTGGATTCATATTACTCATTGCTGATGCCCATCCTCACTTTGACTAGGGCATTGGCTGCTGAGTCCCATATGGATGCTGATCTCGCAAAACCACTTATTAAGTGGGATTTGCTGAAATATGATTTTACGGAAGAGAGACTTTGTCTCTTCGACCGTTATTTTAAATATTGGGACCAGACATACCATCCCAATTGTATTAACTGTTTGGATGATAGGTGTATCCTTCATTGTGCAAACTTTAATGTGTTATTTTCTACTGTGTTTCCACCTACAAGTTTTGGACCACTAGTAAGAAAAATATTTGTAGATGGTGTTCCTTTTGTTGTTTCAACTGGATACCATTTTCGTGAGTTAGGAGTCGTACATAATCAGGATGTAAACTTACATAGCTCGCGTCTCAGTTTCAAGGAACTTTTAGTGTATGCTGCTGATCCAGCTATGCATGCAGCTTCTGGCAATTTATTGCTAGATAAACGCACTACATGCTTTTCAGTAGCTGCACTAACAAACAATGTTGCTTTTCAAACTGTCAAACCCGGTAATTTTAATAAAGACTTTTATGACTTTGCTGTGTCTAAAGGTTTCTTTAAGGAAGGAAGTTCTGTTGAACTAAAACACTTCTTCTTTGCTCAGGATGGCAACGCTGCTATCAGTGATTATGACTATTATCGTTATAATCTGCCAACAATGTGTGATATCAGACAACTCCTATTCGTAGTTGAAGTTGTTGATAAATACTTTGATTGTTACGATGGTGGCTGTATTAATGCCAACCAAGTAATCGTTAACAATCTGGATAAATCAGCTGGTTTCCCATTTAATAAATGGGGTAAGGCTAGACTTTATTATGACTCAATGAGTTATGAGGATCAAGATGCACTTTTCGCGTATACTAAGCGTAATGTCATCCCTACTATAACTCAAATGAATCTTAAGTATGCCATTAGTGCAAAGAATAGAGCTCGCACCGTAGCTGGTGTCTCTATCTGTAGTACTATGACAAATAGACAGTTTCATCAGAAATTATTGAAGTCAATAGCCGCCACTAGAGGAGCTACTGTGGTAATTGGAACAAGCAAGTTTTACGGTGGCTGGCATAATATGTTAAAAACTGTTTACAGTGATGTAGAAACTCCACACCTTATGGGTTGGGATTATCCAAAATGTGACAGAGCCATGCCTAACATGCTTAGGATAATGGCCTCTCTTGTTCTTGCTCGCAAACATAACACTTGCTGTAACTTATCACACCGTTTCTACAGGTTAGCTAACGAGTGTGCGCAAGTATTAAGTGAGATGGTCATGTGTGGCGGCTCACTATATGTTAAACCAGGTGGAACATCATCCGGTGATGCTACAACTGCTTATGCTAATAGTGTCTTTAACATTTGTCAAGCTGTTACAGCCAATGTAAATGCACTTCTTTCAACTGATGGTAATAAGATAGCTGACAAGTATGTCCGCAATCTACAACACAGGCTCTATGAGTGTCTCTATAGAAATAGGGATGTTGATCATGAATTCGTGGATGAGTTTTACGCTTACCTGCGTAAACATTTCTCCATGATGATTCTTTCTGATGATGCCGTTGTGTGCTATAACAGTAACTATGCGGCTCAAGGTTTAGTAGCTAGCATTAAGAACTTTAAGGCAGTTCTTTATTATCAAAATAATGTGTTCATGTCTGAGGCAAAATGTTGGACTGAGACTGACCTTACTAAAGGACCTCACGAATTTTGCTCACAGCATACAATGCTAGTTAAACAAGGAGATGATTACGTGTACCTGCCTTACCCAGATCCATCAAGAATATTAGGCGCAGGCTGTTTTGTCGATGATATTGTCAAAACAGATGGTACACTTATGATTGAAAGGTTCGTGTCACTGGCTATTGATGCTTACCCACTTACAAAACATCCTAATCAGGAGTATGCTGATGTCTTTCACTTGTATTTACAATACATTAGAAAGTTACATGATGAGCTTACTGGCCACATGTTGGACATGTATTCCGTAATGCTAACTAATGATAACACCTCACGGTACTGGGAACCTGAGTTTTATGAGGCTATGTACACACCACATACAGTCTTGCAGGCTGTAGGTGCTTGTGTATTGTGCAATTCACAGACTTCACTTCGTTGCGGTGCCTGTATTAGGAGACCATTCCTATGTTGCAAGTGCTGCTATGACCATGTCATTTCAACATCACACAAATTAGTGTTGTCTGTTAATCCCTATGTTTGCAATGCCCCAGGTTGTGATGTCACTGATGTGACACAACTGTATCTAGGAGGTATGAGCTATTATTGCAAGTCACATAAGCCTCCCATTAGTTTTCCATTATGTGCTAATGGTCAGGTTTTTGGTTTATACAAAAACACATGTGTAGGCAGTGACAATGTCACTGACTTCAATGCGATAGCAACATGTGATTGGACTAATGCTGGCGATTACATACTTGCCAACACTTGTACTGAGAGACTCAAGCTTTTCGCAGCAGAAACGCTCAAAGCCACTGAGGAAACATTTAAGCTGTCATATGGTATTGCCACTGTACGCGAAGTACTCTCTGACAGAGAATTGCATCTTTCATGGGAGGTTGGAAAACCTAGACCACCATTGAACAGAAACTATGTCTTTACTGGTTACCGTGTAACTAAAAATAGTAAAGTACAGATTGGAGAGTACACCTTTGAAAAAGGTGACTATGGTGATGCTGTTGTGTACAGAGGTACTACGACATACAAGTTGAATGTTGGTGATTACTTTGTGTTGACATCTCACACTGTAATGCCACTTAGTGCACCTACTCTAGTGCCACAAGAGCACTATGTGAGAATTACTGGCTTGTACCCAACACTCAACATCTCAGATGAGTTTTCTAGCAATGTTGCAAATTATCAAAAGGTCGGCATGCAAAAGTACTCTACACTCCAAGGACCACCTGGTACTGGTAAGAGTCATTTTGCCATCGGACTTGCTCTCTATTACCCATCTGCTCGCATAGTGTATACGGCATGCTCTCATGCAGCTGTTGATGCCCTATGTGAAAAGGCATTAAAATATTTGCCCATAGATAAATGTAGTAGAATCATACCTGCGCGTGCGCGCGTAGAGTGTTTTGATAAATTCAAAGTGAATTCAACACTAGAACAGTATGTTTTCTGCACTGTAAATGCATTGCCAGAAACAACTGCTGACATTGTAGTCTTTGATGAAATCTCTATGGCTACTAATTATGACTTGAGTGTTGTCAATGCTAGACTTCGTGCAAAACACTACGTCTATATTGGCGATCCTGCTCAATTACCAGCCCCCCGCACATTGCTGACTAAAGGCACACTAGAACCAGAATATTTTAATTCAGTGTGCAGACTTATGAAAACAATAGGTCCAGACATGTTCCTTGGAACTTGTCGCCGTTGTCCTGCTGAAATTGTTGACACTGTGAGTGCTTTAGTTTATGACAATAAGCTAAAAGCACACAAGGATAAGTCAGCTCAATGCTTCAAAATGTTCTACAAAGGTGTTATTACACATGATGTTTCATCTGCAATCAACAGACCTCAAATAGGCGTTGTAAGAGAATTTCTTACACGCAATCCTGCTTGGAGAAAAGCTGTTTTTATCTCACCTTATAATTCACAGAACGCTGTAGCTTCAAAAATCTTAGGATTGCCTACGCAGACTGTTGATTCATCACAGGGTTCTGAATATGACTATGTCATATTCACACAAACTACTGAAACAGCACACTCTTGTAATGTCAACCGCTTCAATGTGGCTATCACAAGGGCAAAAATTGGCATTTTGTGCATAATGTCTGATAGAGATCTTTATGACAAACTGCAATTTACAAGTCTAGAAATACCACGTCGCAATGTGGCTACATTACAAGCAGAAAATGTAACTGGACTTTTTAAGGACTGTAGTAAGATCATTACTGGTCTTCATCCTACACAGGCACCTACACACCTCAGCGTTGATATAAAGTTCAAGACTGAAGGATTATGTGTTGACATACCAGGCATACCAAAGGACATGACCTACCGTAGACTCATCTCTATGATGGGTTTCAAAATGAATTACCAAGTCAATGGTTACCCTAATATGTTTATCACCCGCGAAGAAGCTATTCGTCACGTTCGTGCGTGGATTGGCTTTGATGTAGAGGGCTGTCATGCAACTAGAGATGCTGTGGGTACTAACCTACCTCTCCAGCTAGGATTTTCTACAGGTGTTAACTTAGTAGCTGTACCGACTGGTTATGTTGACACTGAAAATAACACAGAATTCACCAGAGTTAATGCAAAACCTCCACCAGGTGACCAGTTTAAACATCTTATACCACTCATGTATAAAGGCTTGCCCTGGAATGTAGTGCGTATTAAGATAGTACAAATGCTCAGTGATACACTGAAAGGATTGTCAGACAGAGTCGTGTTCGTCCTTTGGGCGCATGGCTTTGAGCTTACATCAATGAAGTACTTTGTCAAGATTGGACCTGAAAGAACGTGTTGTCTGTGTGACAAACGTGCAACTTGCTTTTCTACTTCATCAGATACTTATGCCTGCTGGAATCATTCTGTGGGTTTTGACTATGTCTATAACCCATTTATGATTGATGTTCAGCAGTGGGGCTTTACGGGTAACCTTCAGAGTAACCATGACCAACATTGCCAGGTACATGGAAATGCACATGTGGCTAGTTGTGATGCTATCATGACTAGATGTTTAGCAGTCCATGAGTGCTTTGTTAAGCGCGTTGATTGGTCTGTTGAATACCCTATTATAGGAGATGAACTGAGGGTTAATTCTGCTTGCAGAAAAGTACAACACATGGTTGTGAAGTCTGCATTGCTTGCTGATAAGTTTCCAGTTCTTCATGACATTGGAAATCCAAAGGCTATCAAGTGTGTGCCTCAGGCTGAAGTAGAATGGAAGTTCTACGATGCTCAGCCATGTAGTGACAAAGCTTACAAAATAGAGGAACTCTTCTATTCTTATGCTACACATCACGATAAATTCACTGATGGTGTTTGTTTGTTTTGGAATTGTAACGTTGATCGTTACCCAGCCAATGCAATTGTGTGTAGGTTTGACACAAGAGTCTTGTCAAACTTGAACTTACCAGGCTGTGATGGTGGTAGTTTGTATGTGAATAAGCATGCATTCCACACTCCAGCTTTCGATAAAAGTGCATTTACTAATTTAAAGCAATTGCCTTTCTTTTACTATTCTGATAGTCCTTGTGAGTCTCATGGCAAACAAGTAGTGTCGGATATTGATTATGTTCCACTCAAATCTGCTACGTGTATTACACGATGCAATTTAGGTGGTGCTGTTTGCAGACACCATGCAAATGAGTACCGACAGTACTTGGATGCATATAATATGATGATTTCTGCTGGATTTAGCCTATGGATTTACAAACAATTTGATACTTATAACCTGTGGAATACATTTACCAGGTTACAGAGTTTAGAAAATGTGGCTTATAATGTTGTTAATAAAGGACACTTTGATGGACACGCCGGCGAAGCACCTGTTTCCATCATTAATAATGCTGTTTACACAAAGGTAGATGGTATTGATGTGGAGATCTTTGAAAATAAGACAACACTTCCTGTTAATGTTGCATTTGAGCTTTGGGCTAAGCGTAACATTAAACCAGTGCCAGAGATTAAGATACTCAATAATTTGGGTGTTGATATCGCTGCTAATACTGTAATCTGGGACTACAAAAGAGAAGCCCCAGCACATGTATCTACAATAGGTGTCTGCACAATGACTGACATTGCCAAGAAACCTACTGAGAGTGCTTGTTCTTCACTTACTGTCTTGTTTGATGGTAGAGTGGAAGGACAGGTAGACCTTTTTAGAAACGCCCGTAATGGTGTTTTAATAACAGAAGGTTCAGTCAAAGGTCTAACACCTTCAAAGGGACCAGCACAAGCTAGCGTCAATGGAGTCACATTAATTGGAGAATCAGTAAAAACACAGTTTAACTACTTTAAGAAAGTAGACGGCATTATTCAACAGTTGCCTGAAACCTACTTTACTCAGAGCAGAGACTTAGAGGATTTTAAGCCCAGATCACAAATGGAAACTGACTTTCTCGAGCTCGCTATGGATGAATTCATACAGCGATATAAGCTCGAGGGCTATGCCTTCGAACACATCGTTTATGGAGATTTCAGTCATGGACAACTTGGCGGTCTTCATTTAATGATAGGCTTAGCCAAGCGCTCACAAGATTCACCACTTAAATTAGAGGATTTTATCCCTATGGACAGCACAGTGAAAAATTACTTCATAACAGATGCGCAAACAGGTTCATCAAAATGTGTGTGTTCTGTGATTGATCTTTTACTTGATGACTTTGTCGAGATAATAAAGTCACAAGATTTGTCAGTGATTTCAAAAGTGGTCAAGGTTACAATTGACTATGCTGAAATTTCATTCATGCTTTGGTGTAAGGATGGACATGTTGAAACCTTCTACCCAAAACTACAAGCAAGTCAAGCGTGGCAACCAGGTGTTGCGATGCCTAACTTGTACAAGATGCAAAGAATGCTTCTTGAAAAGTGTGACCTTCAGAATTATGGTGAAAATGCTGTTATACCAAAAGGAATAATGATGAATGTCGCAAAGTATACTCAACTGTGTCAATACTTAAATACACTTACTTTAGCTGTACCCTACAACATGAGAGTTATTCACTTTGGTGCTGGCTCTGATAAAGGAGTTGCACCAGGTACAGCTGTGCTCAGACAATGGTTGCCAACTGGCACACTACTTGTCGATTCAGATCTTAATGACTTCGTCTCCGACGCAGATTCTACTTTAATTGGAGACTGTGCAACAGTACATACGGCTAATAAATGGGACCTTATTATTAGCGATATGTATGACCCTAGGACCAAACATGTGACAAAAGAGAATGACTCTAAAGAAGGGTTTTTCACTTATCTGTGTGGATTTATAAAGCAAAAACTAGCCCTGGGTGGTTCTATAGCTGTAAAGATAACAGAGCATTCTTGGAATGCTGACCTTTACAAGCTTATGGGCCATTTCTCATGGTGGACAGCTTTTGTTACAAATGTAAATGCATCATCATCGGAAGCATTTTTAATTGGGGCTAACTATCTTGGCAAGCCGAAGGAACAAATTGATGGCTATACCATGCATGCTAACTACATTTTCTGGAGGAACACAAATCCTATCCAGTTGTCTTCCTATTCACTCTTTGACATGAGCAAATTTCCTCTTAAATTAAGAGGAACTGCTGTAATGTCTCTTAAGGAGAATCAAATCAATGATATGATTTATTCTCTTCTGGAAAAAGGTAGGCTTATCATTAGAGAAAACAACAGAGTTGTGGTTTCAAGTGATATTCTTGTTAACAACTAAACGAACATGTTTATTTTCTTATTATTTCTTACTCTCACTAGTGGTAGTGACCTTGACCGGTGCACCACTTTTGATGATGTTCAAGCTCCTAATTACACTCAACATACTTCATCTATGAGGGGGGTTTACTATCCTGATGAAATTTTTAGATCAGACACTCTTTATTTAACTCAGGATTTATTTCTTCCATTTTATTCTAATGTTACAGGGTTTCATACTATTAATCATACGTTTGGCAACCCTGTCATACCTTTTAAGGATGGTATTTATTTTGCTGCCACAGAGAAATCAAATGTTGTCCGTGGTTGGGTTTTTGGTTCTACCATGAACAACAAGTCACAGTCGGTGATTATTATTAACAATTCTACTAATGTTGTTATACGAGCATGTAACTTTGAATTGTGTGACAACCCTTTCTTTGCTGTTTCTAAACCCATGGGTACACAGACACATACTATGATATTCGATAATGCATTTAATTGCACTTTCGAGTACATATCTGATGCCTTTTCGCTTGATGTTTCAGAAAAGTCAGGTAATTTTAAACACTTACGAGAGTTTGTGTTTAAAAATAAAGATGGGTTTCTCTATGTTTATAAGGGCTATCAACCTATAGATGTAGTTCGTGATCTACCTTCTGGTTTTAACACTTTGAAACCTATTTTTAAGTTGCCTCTTGGTATTAACATTACAAATTTTAGAGCCATTCTTACAGCCTTTTCACCTGCTCAAGACATTTGGGGCACGTCAGCTGCAGCCTATTTTGTTGGCTATTTAAAGCCAACTACATTTATGCTCAAGTATGATGAAAATGGTACAATCACAGATGCTGTTGATTGTTCTCAAAATCCACTTGCTGAACTCAAATGCTCTGTTAAGAGCTTTGAGATTGACAAAGGAATTTACCAGACCTCTAATTTCAGGGTTGTTCCCTCAGGAGATGTTGTGAGATTCCCTAATATTACAAACTTGTGTCCTTTTGGAGAGGTTTTTAATGCTACTAAATTCCCTTCTGTCTATGCATGGGAGAGAAAAAAAATTTCTAATTGTGTTGCTGATTACTCTGTGCTCTACAACTCAACATTTTTTTCAACCTTTAAGTGCTATGGCGTTTCTGCCACTAAGTTGAATGATCTTTGCTTCTCCAATGTCTATGCAGATTCTTTTGTAGTCAAGGGAGATGATGTAAGACAAATAGCGCCAGGACAAACTGGTGTTATTGCTGATTATAATTATAAATTGCCAGATGATTTCATGGGTTGTGTCCTTGCTTGGAATACTAGGAACATTGATGCTACTTCAACTGGTAATTATAATTATAAATATAGGTATCTTAGACATGGCAAGCTTAGGCCCTTTGAGAGAGACATATCTAATGTGCCTTTCTCCCCTGATGGCAAACCTTGCACCCCACCTGCTCTTAATTGTTATTGGCCATTAAATGATTATGGTTTTTACACCACTACTGGCATTGGCTACCAACCTTACAGAGTTGTAGTACTTTCTTTTGAACTTTTAAATGCACCGGCCACGGTTTGTGGACCAAAATTATCCACTGACCTTATTAAGAACCAGTGTGTCAATTTTAATTTTAATGGACTCACTGGTACTGGTGTGTTAACTCCTTCTTCAAAGAGATTTCAACCATTTCAACAATTTGGCCGTGATGTTTCTGATTTCACTGATTCCGTTCGAGATCCTAAAACATCTGAAATATTAGACATTTCACCTTGCGCTTTTGGGGGTGTAAGTGTAATTACACCTGGAACAAATGCTTCATCTGAAGTTGCTGTTCTATATCAAGATGTTAACTGCACTGATGTTTCTACAGCAATTCATGCAGATCAACTCACACCAGCTTGGCGCATATATTCTACTGGAAACAATGTATTCCAGACTCAAGCAGGCTGTCTTATAGGAGCTGAGCATGTCGACACTTCTTATGAGTGCGACATTCCTATTGGAGCTGGCATTTGTGCTAGTTACCATACAGTTTCTTTATTACGTAGTACTAGCCAAAAATCTATTGTGGCTTATACTATGTCTTTAGGTGCTGATAGTTCAATTGCTTACTCTAATAACACCATTGCTATACCTACTAACTTTTCAATTAGCATTACTACAGAAGTAATGCCTGTTTCTATGGCTAAAACCTCCGTAGATTGTAATATGTACATCTGCGGAGATTCTACTGAATGTGCTAATTTGCTTCTCCAATATGGTAGCTTTTGCACACAACTAAATCGTGCACTCTCAGGTATTGCTGCTGAACAGGATCGCAACACACGTGAAGTGTTCGCTCAAGTCAAACAAATGTACAAAACCCCAACTTTGAAATATTTTGGTGGTTTTAATTTTTCACAAATATTACCTGACCCTCTAAAGCCAACTAAGAGGTCTTTTATTGAGGACTTGCTCTTTAATAAGGTGACACTCGCTGATGCTGGCTTCATGAAGCAATATGGCGAATGCCTAGGTGATATTAATGCTAGAGATCTCATTTGTGCGCAGAAGTTCAATGGACTTACAGTGTTGCCACCTCTGCTCACTGATGATATGATTGCTGCCTACACTGCTGCTCTAGTTAGTGGTACTGCCACTGCTGGATGGACATTTGGTGCTGGCGCTGCTCTTCAAATACCTTTTGCTATGCAAATGGCATATAGGTTCAATGGCATTGGAGTTACCCAAAATGTTCTCTATGAGAACCAAAAACAAATCGCCAACCAATTTAACAAGGCGATTAGTCAAATTCAAGAATCACTTACAACAACATCAACTGCATTGGGCAAGCTGCAAGACGTTGTTAACCAGAATGCTCAAGCATTAAACACACTTGTTAAACAACTTAGCTCTAATTTTGGTGCAATTTCAAGTGTGCTAAATGATATCCTTTCGCGACTTGATAAAGTCGAGGCGGAGGTACAAATTGACAGGTTAATTACAGGCAGACTTCAAAGCCTTCAAACCTATGTAACACAACAACTAATCAGGGCTGCTGAAATCAGGGCTTCTGCTAATCTTGCTGCTACTAAAATGTCTGAGTGTGTTCTTGGACAATCAAAAAGAGTTGACTTTTGTGGAAAGGGCTACCACCTTATGTCCTTCCCACAAGCAGCCCCGCATGGTGTTGTCTTCCTACATGTCACGTATGTGCCATCCCAGGAGAGGAACTTCACCACAGCGCCAGCAATTTGTCATGAAGGCAAAGCATACTTCCCTCGTGAAGGTGTTTTTGTGTTTAATGGCACTTCTTGGTTTATTACACAGAGGAACTTCTTTTCTCCACAAATAATTACTACAGACAATACATTTGTCTCAGGAAATTGTGATGTCGTTATTGGCATCATTAACAACACAGTTTATGATCCTCTGCAACCTGAGCTTGACTCATTCAAAGAAGAGCTGGACAAGTACTTCAAAAATCATACATCACCAGATGTTGATCTTGGCGACATTTCAGGCATTAACGCTTCTGTCGTCAACATTCAAAAAGAAATTGACCGCCTCAATGAGGTCGCTAAAAATTTAAATGAATCACTCATTGACCTTCAAGAATTGGGAAAATATGAGCAATATATTAAATGGCCTTGGTATGTTTGGCTCGGCTTCATTGCTGGACTAATTGCCATCGTCATGGTTACAATCTTGCTTTGTTGCATGACTAGTTGTTGCAGTTGCCTCAAGGGTGCATGCTCTTGTGGTTCTTGCTGCAAGTTTGATGAGGATGACTCTGAGCCAGTTCTCAAGGGTGTCAAATTACATTACACATAAACGAACTTATGGATTTGTTTATGAGATTTTTTACTCTTAGATCAATTACTGCACAGCCAGTAAAAATTGACAATGCTTCTCCTGCAAGTACTGTTCATGCTACAGCAACGATACCGCTACAAGCCTCACTCCCTTTCGGATGGCTTGTTATTGGCGTTGCATTTCTTGCTGTTTTTCAGAGCGCTACCAAAATAATTGCGCTCAATAAAAGATGGCAGCTAGCCCTTTATAAGGGCTTCCAGTTCATTTGCAATTTACTGCTGCTATTTGTTACCATCTATTCACATCTTTTGCTTGTCGCTGCAGGTATGGAGGCGCAATTTTTGTACCTCTATGCCTTGATATATTTTCTACAATGCATCAACGCATGTAGAATTATTATGAGATGTTGGCTTTGTTGGAAGTGCAAATCCAAGAACCCATTACTTTATGATGCCAACTACTTTGTTTGCTGGCACACACATAACTATGACTACTGTATACCATATAACAGTGTCACAGATACAATTGTCGTTACTGAAGGTGACGGCATTTCAACACCAAAACTCAAAGAAGACTACCAAATTGGTGGTTATTCTGAGGATAGGCACTCAGGTGTTAAAGACTATGTCGTTGTACATGGCTATTTCACCGAAGTTTACTACCAGCTTGAGTCTACACAAATTACTACAGACACTGGTATTGAAAATGCTACATTCTTCATCTTTAACAAGCTTGTTAAAGACCCACCGAATGTGCAAATACACACAATCGACGGCTCTTCAGGAGTTGCTAATCCAGCAATGGATCCAATTTATGATGAGCCGACGACGACTACTAGCGTGCCTTTGTAAGCACAAGAAAGTGAGTACGAACTTATGTACTCATTCGTTTCGGAAGAAACAGGTACGTTAATAGTTAATAGCGTACTTCTTTTTCTTGCTTTCGTGGTATTCTTGCTAGTCACACTAGCCATCCTTACTGCGCTTCGATTGTGTGCGTACTGCTGCAATATTGTTAACGTGAGTTTAGTAAAACCAACGGTTTACGTCTACTCGCGTGTTAAAAATCTGAACTCTTCTGAAGGAGTTCCTGATCTTCTGGTCTAAACGAACTAACTATTATTATTATTCTGTTTGGAACTTTAACATTGCTTATCATGGCAGACAACGGTACTATTACCGTTGAGGAGCTTAAACAACTCCTGGAACAATGGAACCTAGTAATAGGTTTCCTATTCCTAGCCTGGATTATGTTACTACAATTTGCCTATTCTAATCGGAACAGGTTTTTGTACATAATAAAGCTTGTTTTCCTCTGGCTCTTGTGGCCAGTAACACTTGCTTGTTTTGTGCTTGCTGCTGTCTACAGAATTAATTGGGTGACTGGCGGGATTGCGATTGCAATGGCTTGTATTGTAGGCTTGATGTGGCTTAGCTACTTCGTTGCTTCCTTCAGGCTGTTTGCTCGTACCCGCTCAATGTGGTCATTCAACCCAGAAACAAACATTCTTCTCAATGTGCCTCTCCGGGGGACAATTGTGACCAGACCGCTCATGGAAAGTGAACTTGTCATTGGTGCTGTGATCATTCGTGGTCACTTGCGAATGGCCGGACACTCCCTAGGGCGCTGTGACATTAAGGACCTGCCAAAAGAGATCACTGTGGCTACATCACGAACGCTTTCTTATTACAAATTAGGAGCGTCGCAGCGTGTAGGCACTGATTCAGGTTTTGCTGCATACAACCGCTACCGTATTGGAAACTATAAATTAAATACAGACCACGCCGGTAGCAACGACAATATTGCTTTGCTAGTACAGTAAGTGACAACAGATGTTTCATCTTGTTGACTTCCAGGTTACAATAGCAGAGATATTGATTATCATTATGAGGACTTTCAGGATTGCTATTTGGAATCTTGACGTTATAATAAGTTCAATAGTGAGACAATTATTTAAGCCTCTAACTAAGAAGAATTATTCGGAGTTAGATGATGAAGAACCTATGGAGTTAGATTATCCATAAAACGAACATGAAAATTATTCTCTTCCTGACATTGATTGTATTTACATCTTGCGAGCTATATCACTATCAGGAGTGTGTTAGAGGTACGACTGTACTACTAAAAGAACCTTGCCCATCAGGAACATACGAGGGCAATTCACCATTTCACCCTCTTGCTGACAATAAATTTGCACTAACTTGCACTAGCACACACTTTGCTTTTGCTTGTGCTGACGGTACTCGACATACCTATCAGCTGCGTGCAAGATCAGTTTCACCAAAACTTTTCATCAGACAAGAGGAGGTTCAACAAGAGCTCTACTCGCCACTTTTTCTCATTGTTGCTGCTCTAGTATTTTTAATACTTTGCTTCACCATTAAGAGAAAGACAGAATGAATGAGCTCACTTTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATAATGCTTATTATATTTTGGTTTTCACTCGAAATCCAGGATCTAGAAGAACCTTGTACCAAAGTCTAAACGAACATGAAACTTCTCATTGTTTTGACTTGTATTTCTCTATGCAGTTGCATATGCACTGTAGTACAGCGCTGTGCATCTAATAAACCTCATGTGCTTGAAGATCCTTGTAAGGTACAACACTAGGGGTAATACTTATAGCACTGCTTGGCTTTGTGCTCTAGGAAAGGTTTTACCTTTTCATAGATGGCACACTATGGTTCAAACATGCACACCTAATGTTACTATCAACTGTCAAGATCCAGCTGGTGGTGCGCTTATAGCTAGGTGTTGGTACCTTCATGAAGGTCACCAAACTGCTGCATTTAGAGACGTACTTGTTGTTTTAAATAAACGAACAAATTAAAATGTCTGATAATGGACCCCAATCAAACCAACGTAGTGCCCCCCGCATTACATTTGGTGGACCCACAGATTCAACTGACAATAACCAGAATGGAGGACGCAATGGGGCAAGGCCAAAACAGCGCCGACCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACAGCTCTCACTCAGCATGGCAAGGAGGAACTTAGATTCCCTCGAGGCCAGGGCGTTCCAATCAACACCAATAGTGGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCCGACGAGTTCGTGGTGGTGACGGCAAAATGAAAGAGCTCAGCCCCAGATGGTACTTCTATTACCTAGGAACTGGCCCAGAAGCTTCACTTCCCTACGGCGCTAACAAAGAAGGCATCGTATGGGTTGCAACTGAGGGAGCCTTGAATACACCCAAAGACCACATTGGCACCCGCAATCCTAATAACAATGCTGCCACCGTGCTACAACTTCCTCAAGGAACAACATTGCCAAAAGGCTTCTACGCAGAGGGAAGCAGAGGCGGCAGTCAAGCCTCTTCTCGCTCCTCATCACGTAGTCGCGGTAATTCAAGAAATTCAACTCCTGGCAGCAGTAGGGGAAATTCTCCTGCTCGAATGGCTAGCGGAGGTGGTGAAACTGCCCTCGCGCTATTGCTGCTAGACAGATTGAACCAGCTTGAGAGCAAAGTTTCTGGTAAAGGCCAACAACAACAAGGCCAAACTGTCACTAAGAAATCTGCTGCTGAGGCATCTAAAAAGCCTCGCCAAAAACGTACTGCCACAAAACAGTACAACGTCACTCAAGCATTTGGGAGACGTGGTCCAGAACAAACCCAAGGAAATTTCGGGGACCAAGACCTAATCAGACAAGGAACTGATTACAAACATTGGCCGCAAATTGCACAATTTGCTCCAAGTGCCTCTGCATTCTTTGGAATGTCACGCATTGGCATGGAAGTCACACCTTCGGGAACATGGCTGACTTATCATGGAGCCATTAAATTGGATGACAAAGATCCACAATTCAAAGACAACGTCATACTGCTGAACAAGCACATTGACGCATACAAAACATTCCCACCAACAGAGCCTAAAAAGGACAAAAAGAAAAAGACTGATGAAGCTCAGCCTTTGCCGCAGAGACAAAAGAAGCAGCCCACTGTGACTCTTCTTCCTGCGGCTGACATGGATGATTTCTCCAGACAACTTCAAAATTCCATGAGTGGAGCTTCTGCTGATTCAACTCAGGCATAAACACTCATGATGACCACACAAGGCAGATGGGCTATGTAAACGTTTTCGCAATTCCGTTTACGATACATAGTCTACTCTTGTGCAGAATGAATTCTCGTAACTAAACAGCACAAGTAGGTTTAGTTAACTTTAATCTCACATAGCAATCTTTAATCAATGTGTAACATTAGGGAGGACTTGAAAGAGCCACCACATTTTCATCGAGGCCACGCGGAGTACGATCGAGGGTACAGTGAATAATGCTAGGGAGAGCTGCCTATATGGAAGAGCCCTAATGTGTAAAATTAATTTTAGTAGTGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAATGACAAAAAAAAAAAAAAAAAAAAAAAA zcrb1_human Zinc finger CCHC-type and RNA-binding motif-containing protein 1 ZCRB1 U11/U12 small nuclear ribonucleoprotein 31 kDa protein protein protein human Homo sapiens Human MSGGLAPSKSTVYVSNLPFSLTNNDLYRIFSKYGKVVKVTIMKDKDTRKSKGVAFILFLDKDSAQNCTRAINNKQLFGRVIKASIAIDNGRAAEFIRRRNYFDKSKCYECGESGHLSYACPKNMLGEREPPKKKEKKKKKKAPEPEEEIEEVEESEDEGEDPALDSLSQAIAFQQAKIEEEQKKWKPSSGVPSTSDDSRRPRIKKSTYFSDEEELSD rna_ibv Infectious bronchitis virus complete genome rna ribonucleic acid RNA rna ibvb ibvb IBV ACTTAAGATAGATATTAATATATATCTATTGCACTAGCCTTGCGCTAGATTTCCAACTTAACAAAACGGACTTAAATACCTACAGCTGGTCCCCATAGGTGTTCCATTGCAGTGCACTTTAGTGCCCTGGATGGCACCTGGCCACCTGTCAGGTTTTTGTTGCTAAAATATCATTGTTGCTGGTATCACTGCTTGTTTTGCCGTGTCTCACTTTATACATCCGTTGCTTGGGCTACCTAGTATCCAGCGTCCTACGGGCGCCGTGGTCGGTTCGAGTGCGAAGGACCTCTGGTTCATCTAGCGGTAGGCGGGTGTGTGGAAGTAGCGCTTCAGACGTATTGGTTCTGTTGCGTGAAACGCGGGGTCACCTCCCCCCACATACCTCTAAGGGCTTTTGAGCCTAGCGTTGGGCTACGTTCTCGCACAAGGTCGGCTATACGACGTTTGTAGGGGGTAGTGCCAAACAACCCCTGAGGTGACAGGTTCTGGTGGTGTTTAGTGAGCAGACATACAATAGACAGTGACAACATGGCTTCAAGCCTAAAACAGGGAGTATCTCCCAAACCAAGGGATGTCATTCTTGTTTCCAAAGACATTCCCGAACAACTCTGTGACGCTTTATTTTTCTACACGTCACATAACCCTAAGGATTACGCTGATGCTTTTGCATTTAGGCAAAAGTTTGACCGTAATCTGCAGACTGGGAAGCAGTTCAAATTTGAAACTGTTTGTGGTCTCTTCCTATTGAAGGGAGTTGACAAAATAACACCTGGCGTCCCAGCAAAAGTTTTAAAAGCCACTTCTAAGTTGGCAGATTTAGAAGACATCTTTGGTGTCTCTCCTTTTGCACGGAAGTACCGTGAATTGTTGAAAACAGCATGCCAGTGGTCTCTTACTGTAGAAACACTGGATGCTCGTGCACAAACGCTTGACGAAATTTTTGACTCTACTGAAATACTTTGGCTTCAGGTGGCTGCAAAAATTCAAGTTTCAGCTATGGCAATGCGCAGGCTTGTTGGAGAAGTAACTGCAAAAGTCATGGAAGCTCTTGGCTCAAATTTGAGTGTTCTCTTTCAAATTGTTAAACAACAAATAGCCAGAATCTTTCAAAAGGCACTGGCTATTTTTGAAAATGTGAGTGAATTACCACAGCGTATTGCAGCACTTAAGATGGCCTTTGCCAAGTGTGCCAAGTCAATTACTGTTGTGGTTGTGGAAAGAACTCTAGTTGTTAGAGAGTTCGCAGGAACTTGTCTTGCAAGCATCAATGGTGCTGTTGCAAAATTCTTTGAAGAACTTCCAAATGGCTTCATGGGTTCTAAAATCTTCACAACATTGGCCTTCTTTAAAGAAGCAGCTGTGAAAATTGTGGAAAATATACCAAATGCACCAAGAGGTACTAGAGGTTTTGAAGTCGTTGGTAACGCCAAGGGAACGCAAGTTGTTGTGCGTGGCATGCGAAATGATTTAACTCTGCTCGACCAAAAAGCTGACATTTCTGTTGAGAAAGAAGGTTGGTCTGCAATTCTTGAAGGACATCTGTGTTATGTCTTTAAGAGTGGTGATCGTTTTTATGCGGCACCTCTTTCTGGGAATTTTGCATTGCATGATGTGCATTGTTGTGAGCGTGTTGTCTGTCTGTCTGATGGTGTAACACCAGAGATAAATGATGGACTCATTCTAGCAGCAATCTATTCATCTTTTAGTGTCTCAGAACTCGTGGCAGCACTTAAAAAGGGTGAACCATTCAAGTTCTTGGGTCATAAATTTGTGTATGCGAAGGATGCAGCAGTCTCTTTCACTCTTGCAAAAGCAGCCACTATTGCAGATGTACTGAAGCTGTTTCAATCAGCTCGTGTGCAAACGGAAGATGTGTGGTCTGCATTTACTGAAAAGTCTTTTAATTTCTGGAAACTCGCATATGGAAAAGTGCGTAATCTTGAAGAAGTTGTGAAGACTCATTTTTGTAAAGCTCAAATGTCAATTATCATTCTAGCAGCAGTGCTTGGCGAAGGCATTTGGCATCTTGTTTCACAGGTCATCTATAAAGTAGGTGGTCTTTTTACTAGAGTCGTTGACTTTTGTGAAAAACACTGGAAGGGTTTCTGTGCACAACTTAAAAAGGCTAAGCTCGTTGTCACAGAAACTCTTTGTGTTCTTAAGGGAGTGGCACAGCATTGTTTTCAACTATTGCTGGATGCAATACATTCTTTGTATATGAGTTTTAAGAAGTGTGCACTTGGTAGAATTCATGGAGACTTACTCTTCTGGAAAGGGGGTGTACACAAAATTGTTCAAGATGGCGATGAAGTTTGGTTTGACGCCATTGATAGTATTGATGTTGAAGATCTGGGTGTTGTCCAAGAAAAACCCATAGATTTTGAGGTTTGTGAAGACGTAACACTTCCAGAAAATCAACCTGGTCATATGGTTCAAATCGAGGATGACGGAAAGAACTATATGTTCTTCCGCTTCAAAAGGGATGAGAACATCTACTATACACCAATGTCTCAACTTGGTGTAATTAATGTAGTTTGCAAAGCAGGCGGTAAAACCGTTACCTTTGGAGACACCATTGTGAAAGAAATACCGCCACCTGATGTTGTGCCTATTAAGGTTAGCATAGAGTGTTGTGGTGAACCATGGAATACAATCTTCAAGAAAGCTTATAAAGAGCCCATTGAAGTTGAAACTGACCTCACAGTAGAACAATTGCTCTCTGTGATCTATGAGAAAATGTGCGACGACCTCAAATTGTTTCCAGAGGCACCAGAGCCACCGCCATTTGAGAATGTCGCACTTGTTGATAAAAACGGTAAAGACTTGGATTGCATAAAATCATGCCATCTTATCTACCGTGATTATGAGAGTGATGATGACATCGAGGAAGAAGATGCTGAGGAGTGTGATACTGATTTAGAATGTGAAGAAGAGGATGAGGATACTAAAGTGTTGGCTCTTATACAAGACCCTGCAAGTAATAAATACCCTCTTCCTCTTGATGATGATTATAGCGTCTTTAATGGATGTATTGTACATAAGGACGCTCTTGACGTCGTAAATCTACCATCTGGTGAAGAAACCTTTGTTGTCAACAACTGCTTTGAGGGAGCTGTAAAACCACTGCCTCAGAAAGTTGTTGATGTTCTAGGTGACTGGGGTGAGGCTGTTGATGCGCAAGAGCAAATTGCACAAACTACTTCAGAGGAAACCCCTATCAGTAGTTTGGAGGCAACTATTGAGCAAGTTGTTGTTGAGGAACAGAAAATAATTTCTGTTGTTGAAGAAGAACAGCAGGTGGCGGTCTACACACCTGCAGACCTACAAGTTGGTGAAGAAACACCAGATGAGTTTATTCTTATTGCTGATGTTTCCACAGAAGAAATTGTGCCTCATGAAGAAAAGGAGTCACAGATTGAACAGGAGCCTATTCAAGTTGTTAAATCACAACGTGAAAAGAAGGCTAAAAAGTTCAAGGTTAAATCTACTACATGTGAGAAACCCAAATTTTTGGAGTACACAACATGTGTGGGTGACCTAACGGTAGTGATTGCCAAAGCATTGGATGAGTTTAAAGAGTTCTGCATTGTAAATGCTGCTAATGAGCATATGTCTCACGGTGGCGGCGTTGCTAAGGCAATTGCGGACTTTTGTGGACCTGATTTTGTGGAGTATTGTGAGGACTATGTTAAGAAACATGGGCCTCAACAAAGACTTGTCACACCTTCATTTGTCAAAGGCATTCAATGTGTGAACAATGTTGTAGGACCTCGCCATGGAGACAGTAACTTGCATGATAAGCTTGTTGCTGCTTACAAGAATGTTCTTGTAGATGGTGTTGTCAATTATGTTGTGCCAGTCCTCTCATCAGGAATTTTTGGTGTTGATTTTAAGATGTCTATAGACGCTATGCGCAAGGCTTTTGAAGGTTGCGACATACGCGTTCTTCTTTTCTCCTTGTCTCAAGAACACATCGATTATTTCGATGTTACTTGTAAACAGAAGACAATTTATCTTACAGAGGACGGTGTTAAATACCGCTCTGCTACTGTGAAACCAGGTGACTCTTTGAGTCAATTTGGACCGGTTTTTGCTAGAAACAAGACAGTCTTTACAGCAGACGATGTTGAGGATAAAGAAATTCTCTTCATTCCTACTACTGACAAGACTGTCCTTGAATATTATGGTTTGGATGCGCAAAAGTATGTAATATACTTGCAAACTCTTGCACAGAAGTGGAATGTCCAATATAGGGACAATTTTGTTATACTTGAGTGGCGTGATGGAAATTGCTGGATTAATGCAGCAGTAGTGCTCCTTCAAGCTGCTAAGATTAGGTTTAAAGGTTTTCTTGCAGAAGCATGGGCACAACTTTTGGGTGGAGACCCAACTGATTTTGTGGCCTGGTGCTATGCAAGTTGCAATGCTAATGTTGGTGAGTTTTCAGATGCTAATTGGCTTCTTGCTAATTTGGCAGAATACTTTGATGCTGATTACACGAATGCATTCCTTAAGAGGCGTGTGTCATGTAACTGTGGGGTTAAGAATTGTGAAGTTAGAGGCCTTGAAGCTTGTATTCAACCAGTAAAGGCACCCAATCTTCTTCATTTTAAGACTCAGTACACAAATTGTACAGTGTGTGATGCAAATATTGTGGATGAGGTGGTAGAAGCCTCACTACCATATCTGTTGCTCCTTGCTACTGATGGTCCTACTACAGTGGATTGTGATGAAAATGCTGTAGGGAATGTTGTTTTCATTGGCTCTACTAATAGTGGCCATTGTTACACGCAAGCCATTGGTAAGGCTTTTGATAATCTTGCTAAGGATAGAAAATTTTCAAAGAATTCGCCATACATTACAGCAATGTATACGCGCTTCTCTCTTAAGAGTGAAAGCTCTCTGTCTGTTGTTAAACAGAGTAAGAGTAAAACTAAAGTAGTAAAAGAAGATGTTGCCAACCTTGCTACTAGTTCTAAAGCCAGTTTTGATGATCTTACTGACTTTGAACATTGGTATGATAGTAACATCTATGAAAGTCTTAAAGTTCAGGAAATACCTGTGAATTTGGATGAGTATGTGTCATTTACAACGAAAGAAGATACTAAGTTGCCACTGACACTTAAAGTTAGAGGTATCAAATCAGTTGTTGACTTTATTTCAAGAGACGGTTTCTCTTATAAGTTAACACCTGACATTGAAGAAAATTCAAAAGCGCCAGTCTACTACCCAGTCTTAGACTCTATTAGTCTTAAGGCAATATGGGTAGACGGCAGTGCTAATTTTGTTGTTGGTCATCCAAACTACTATAGTAAGTCTCTTCGCATTCCTACTTTTTGGGAAAATGCAGAGAGCTTTGTTAAGATAGGTGACAAAGTTGATGGTGTAACTATGGGCCTTTGGCGTGCAGAACATCTTAACAAACCTAATCTTGAAAGAATTTTCAACATTGCTAAGAAAGCTATTGTTGGATCCAGTGTTGTTACTACACAATGTAGTAAATTAATTAGTAAAGCAGCTACATTCATTGCTGATAAAGTAGGTGGGGGTGTAGTTCGTAATATTACAGATAGAATTAAGGGTCTTTGTGGATTTACACGTGGGCATTTTGAAAGAAAATTGTCTCCACAATTCATAAAAACACTTATATTCTTCTTCTTTTACTTTGTAAAGGCTAGTGCTAAGAGTGTTGCCACTAGTTATAAGCGTGTGTTATGTAAGGTGGTTTTTACCACGCTATTTATATTATGGTTTATGTACACAAGTAAACCAGTAACTTTTACTGGAACACGTGTGCTAGACTTCTTATTTGAGGGTTCTTTATGTGGTCCCTATAATGACTATGGTAAAGACTCATTTGACGTACTACGCTATTGTGGAGATGATTTTACTTGTCGTGTATGTTTACATGATAAAGATTCACTTCATTTGTATAAGCATGCTTATAGCGTAGAACAGGTTTATAAATATGCAGCTTCTGGCATTAGTTTTAATTGGAATTGGCTTTATTTGGTCTTTCTAATATTATTTGTTAAACCAGTAGCAGGTTTCGTTATTATTTGCTATTGTGTTAAGTACTTGGTATTGAGTTCAACTGTGTTGCAAACTGGTGTAGGTTTTATGGACTGGTTTATTCAAACAGTTTTTACTCACTTTAATTTTATGGGTGCAGGTTTCTATTTCTGGCTCTTCTATAAATTGTACATACAGGTTCATCATATACTGTATTGTAAGGATATAACATGTGAAGTGTGTAAGAGAGTTGCACGCAGTAATAGGCATGAGGTTAGTGTTGTTGTTGGTGGACGCAAGCAAATTGTGCACGTGTACACTAACTCTGGTTACAACTTTTGTAAGAGACATAATTGGTATTGTAGGAATTGTGATGTATATGGTCACCAAAACACATTTATGTCTCCTGAAGTTGCTGGCGAGCTTTCTGAAAAGCTTAAACGCCATGTTAAACCTACAGCACATGCTTACCACGTTGTGGATGAGGCTTGCGTAGTTGATGATTTTGTTAACTTAAAATACAAAGCTGCAACTCCTGGTAAGGATGGTGCACCTCCTGCAGTTAAATGTTTCAGTGTTACAGATTTCTTGAAGAAAGCTGTTTTTCTTAAGGATGCGCTGAAATGTGAACAAATATCTAATGATGGTTTTATAGTGTGTAATACGCAGAGTGCGCATGCTTTAGAGGAAGCAAAGAATGCAGCCATCTATTATGCGCAATACCTGTGTAAACCTATACTTATACTCGACCAGGCACTCTACCAGAATTTAATAGTGGAACCTGTATCGAAGAGCGTTGTCAACAAAGTGTGTGACATTTTGTCTAGGATAATTTCTGTAGATACTGCATCTTTGGATTATAAAGCAGGTACAATTCGTGATGCCTTGCTGTCTGTTACTAAAGATGAAGAAGCTGTAGATATGGCTATCTTCTGTCATAATCATGAAGTTGAATATACAGGTGATGGTTTTACTAATGTTATACCGTCATATGGTATAGACACTGATAAATTAACACCTCGTGATAGAGGGTTTTTGATAAATGCAGATGCTTCTGTTGCTAACTTAAGAGTTAAAAATGCTCCGCCGGTAGTATGGAAGTTCTCTGATCTTATTAAGTTGTCTGACAGTTGTCTTAAATATTTAATCTCAGCAACTGTCAAGTCAGGGTCTCGTTTCTTTATAACAAGATCTGGTGCTAAACAAATTTTTTCTTGTAGTACTCAGAAATTGTTGGTAGAGAAAAAGGCTGGTGGTGTCGTTAGTGGTACCTTTAATTGGTTTAAGAGTTGTTGTAAATGGCTCTTGATCTTCTATGTGCTTTTTACATTGTGTTGTTTGGGTTGTTATCATATGGAGACGAATAAAAGTTTTGTTCATCCTATGTATGATGTTAACTCTACAATGCATGTTGAAGGCTTTAAGGTTATAGATAAAGGTGTTATTAGAGACATTGTACCAGAGGATGCTTGTTTCTCTAATAAGTTTGCTAACTTTGATGCATTTTGGGGTAAACCATATGTGAATAGTAGAGACTGTCCAATTGTTACAGCAGTCATAGATGGCGCTGGAACAATAGTAGCTGGTGTTCCTGGTTTTGTAGACTGGGTTCTTGATGGTGTTATGTTTGTACACATGACACAAACAGAAAGAAAACCCTGGTACATTCCCATGTGGTTTAACAGAGAAATTGTTGGTTACACTCAGGATTCAATTATTACTGAAGGTAGTTTTTATACATCTATAGCTTTGTTTTCAGCTAGGTGTTTATATTTAACAGCCAGCAATACACCACAATTGTATTGTTTTAATGGTCATAATGATGCTCCTGGAGCCTTACCATTTAGCAGTATCACTTCACACAGGGTCTACTTCCAACCAAATGGTGTTAGGCTTATAATTCCTCAACAAATAATGCACACACCCTACGTAGTAAAGTTTTTATCAGACAGCTATTGTAGAGGTAGTGTATGTGAGTATACTAAACCGGGTTATTGTGTTTCACTAAATTCCCAATGGGTTTTATTTAATGACGAATACACAAGTAAACCAGGAGTATTCTGTGGTTCTACTGTTAGAGAACTTATGTTTAATATGGTTAGTACATTTTTTACTGGTGTCAACCCTAATATTTATATGCAGCTGGCGACTATGTTTTTAATACTAGTTGTTGTTGTGTTAATTTTTGCAATGGTTATAAAGTTTCAATGTGTTTTTAAAGCTTATGCAACCATTGTGTTTATAATAATGCTAGTTTGGGTTGTTAATGCATTTATTTTGTGTGTACATAGTTATAATAGTGTTGTGGCTGTTATACTACTAGTAATCTATTGTTATGCATCATTGGTTACAAGTCGTAATACTGCTATAATAATGCATTGTTGGCTTGTGTTTACCTTTGGTTTAATTGTACCCATATGGTTGGCGTGTTGCTACCTGGCATTTGTTTTATATATGTACACACCATTGTTTTTCTGGTGTTACGGTACTACTAAAAATACTCGTAAGTTGTATGATGGCAACGAGTTTGTTGGTAATTATGACCTTGCTGCGAAGAGCACTTTTGTTATTCGTGGTACTGAATTTGTTAAGCTTACGAATGAGATAGGTGATAAATTTGAATCCTATCTTTCTGCGTATGCTAGACTTAAATATTATTCAGGCACTGGCAGTGAGCAAGATTACTTGCAAGCCTGTCGTGCATGGTTAGCTTATGCTTTGGACCAATATAGAAATAGTGGTGTGGAAATTGTGTATACTCCACCACGTTACTCTATTGGTGTTAGTAGATTACAGGCTGGTTTTAAGAAACTAGTTTTTCCTAGTAGTGCTGTTGAAAAGTGCATTGTTAGTGTCTCTTATAGAGGTAATAATCTTAATGGACTATGGCTAGGTGATACTATCTACTGTCCGCGACATGTTCTAGGCAAGTTTTCAGGTGATCAATGGAGTGATGTACTTAATCTTGCTAATAATCATGAGTTTGAGGTTGCAACTCAAAATGGTGTTACTTTGAATGTTGTTAGTAGGCGGTTGAGAGGCGCAGTTTTAATTTTACAAACTGCTGTCGCCAATGCTGACACTCCTAAGTATAAGTTTGTTAAAGCTAATTGTGGTGATAGTTTCACTATAGCTTGTTCTTATGGTGGTACAGTTGTGGGACTCTACCCTGTTACTATGCGTTCTAATGGTACTATTAGAGCTTCTTTCCTTGCAGGAGCTTGTGGCTCAGTTGGTTTTAATATAGAGAAGGGTGTAGTTAATTTCTTTTATATGCACCATCTTGAGTTACCTAATGCATTACACACTGGAACTGACCTAATGGGTGATTTCTATGGTGGTTATGTGGACGAAGAGGTTGCACAAAGGGTGCCACCAGATAATTTAGTTACTAATAATATTGTAGCATGGCTTTATGCCGCAATTATTAGTGTTAAGGAGAGTAGTTTCTCACTGCCTAAATGGTTGGAGAGTACTACTGTCAGTGTTGAAGACTATAATAAGTGGGCTGGTGATAATGGTTTTACACCATTTTCTACTAGTACTGCTATTACTAAATTAAGTGCTATAACGGGAGTAGATGTTTGTAAACTCCTTCGCACTATTATGGTAAAAAGTAGTCAATGGGGTAGTGATCCCATTTTAGGACAATATAATTTTGAAGATGAATTGACACCAGAGTCTGTTTTCAACCAGATAGGTGGTGTTAGGTTACAGTCATCTATTGTAAGAAGAGTCACATCTTGGTTTTGGAGTAGATGTGTGTTAGCTTGCTTCTTATTTGTGTTGTGTGCTATTGTCTTGTTTACGGCAGTACCACTTAAATACTATGTACATGCAGCTGTTATTTTGTTAACAGCTGTACTTTTTATTTCTTTTACTGTTAAACATGTTATGGCATATATGGATACTTTTCTGTTGCCTACATTGATTACAGTTATTATTGGAGTTTGTGCTGAAGTCCCTTTCATATACAATACTCTAATTAGTCAAGTTGTTATTTTCTTAAGCCAATGGTATGATCCTGTAGTCTTTGATACTATGGTACCATGGATGTTATTGCCATTAGTGTTGTACACTGCTTTTAAGTGTGTACAAGGTTGCTATATGAATTCTTTCAATACTTCTTTGTTAATGCTGTATCAGTTTATGAAGTTAGGTTTTGTTATTTATACCTCTTCTAACACTCTTACTGCATATACAGAAGGTAATTGGGAGTTATTTTTTGAGTTAGTTCACACTACTGTGTTGGCTAATGTTAGTAGCAATTCTTTAATTGGTCTACTTGTGTTTAAGTGTGCTAAGTGGATGTTGTATTATTGCAATGCAACATACTTTAATAATTATGTGTTAATGGCAGTCATGGTTAATGGCATAGGCTGGCTTTGTACTTGTTACTTTGGATTGTATTGGTGGGTTAATAAGGTTTTTGGTTTAACTTTAGGTAAATACAATTTTAAAGTCTCAGTAGATCAATATAGGTATATGTGTTTGCATAAGATAAATCCACCTAAAACTGTGTGGGAAGTCTTTTCGACAAATATACTTATACAAGGAATTGGTGGTGATCGTGTGTTGCCTATTGCTACAGTTCAATCTAAATTGAGTGATGTAAAGTGTACAACTGTTGTTTTAATGCAGCTTTTGACTAAGCTTAATGTTGAAGCAAATTCAAAAATGCATGCTTATCTTGTTGAGTTACACAATAAAATCCTTGCATCTGATGATGTTGGAGAGTGCATGGATAATTTGTTGGGTATGCTTATTACACTGTTTTGTATAGATTCTACTATTGATTTGAGTGAGTATTGTGATGATATACTTAAGAGGTCAACTGTCTTACAGTCAGTTACTCAAGAGTTCTCACACATACCCTCTTATGCTGAATATGAAAGAGCTAAGAATCTTTATGAAAAGGTTTTAACTGATTCTAAAAATGGTGGTGTAACACAGCAAGAGCTTGCTGCATATCGTAAAGCTGCCAATATTGCAAAGTCAGTTTTTGATAGAGACTTGGCTGTTCAAAAGAAGTTAGACAGCATGGCAGAACGTGCTATGACAACAATGTATAAAGAGGCGCGTGTAACTGATAGACGAGCAAAATTAGTTTCATCACTACATGCGTTACTCTTTTCAATGCTTAAGAAAATAGATTCTGAAAAGCTTAATGTCTTATTTGATCAGGCTAGTAGCGGTGTTGTACCTCTAGCTACTGTTCCAATTGTTTGTAGTAATAAGCTTACCCTTGTAATACCAGATCCAGAAACTTGGGTCAAGTGTGTGGAAGGTATGCATGTTACATATTCAACAGTTGTTTGGAATATAGACACTGTTATTGATGCTGATGGTACAGAGTTACATCCAACTTCTATAGGTAGTGGATTGACATACTGTATAAGTGGTGACAATATAGCATGGCCTTTAAAGGTCAACTTGACTAGGAATGGGCATAACAAGGTTGATGCTGCTTTGCAGAATAATGAGCTTATGCCTCATGGTGTAAAAACAAAGGCTTGCGTAGCAGGTGTAGATCAAGCACATTGTAGCGTAGAGTCTAAATGTTATTATACAAATATTAGTGGCAATTCAGTTGTAGCTGCTATTACTTCTTCAAATCCAAATCTGAAAGTAGCTTCGTTTTTGAACGAGGCAGGCAATCAGATTTATGTAGACTTAGACCCACCATGTAAATTTGGCATGAAGGTGGGTGACAAGGTTGAGGTTGTTTACTTGTATTTTATAAAGAATACAAGGTCGATTGTTAGGGGTATGGTACTTGGTGCTATATCTAATGTTGTTGTCTTACAGTCTAAAGGGCATGAAACAGAGGAAGTGGATGCTGTTGGCATTCTTTCACTTTGCTCATTTGCAGTAGATCCCGCTGATACATATTGTAAATATGTGGCGGCAGGTAATCAACCTTTAGGTAACTGTGTTAAAATGTTGACAGTACATAATGGTAGTGGCTTTGCTATAACATCAAAGCCAAGTCCAACTCCTGATCAGGATTCTTATGGAGGAGCTTCTGTGTGTCTCTATTGTAGAGCACACATAGCACACCCAGGAGGTGCAGGAAATTTAGATGGACGTTGTCTATTTAAAGGTTCTTTTGTGCAAATACCTACTACTGAGAAAGACCCCGTCGGATTCTGTCTACGTAATAAGGTTTGTACTGTTTGTCAGTGTTGGATTGGTTATGGCTGTCAGTGCGATGCACTTAGACAACCTAAACCTTTTGTTCAGTCAGTTGCTGGTGCATCTGATTTTGATAAGAATTATTTAAACGGGTACGGGGTAGCAGTGAGGCTCGGCTGATACCCCTTGCTAGTGGATGTGATCCTGATGTTGTAAAGCGAGCCTTTGATGTTTGTAATAAGGAATCATCTGGTATGTTTCGAAACTTTAAGCGTAACTGTGCGAGATTCCAAGAAGTACGTGATACTGAAGATGGAAATCTTGAGTATTGTGATTCGTACTTTGTGGTTAAACAAACCACTCCTAGTAATTATGAACATGAGCGGTCTTGTCATGAAGACTTAAAGTCAGACGTAATAGCCGATCATGATTTCTTTGTGTTCAATAAGAACATTTATAATATTAGTAGGCAGAGGCTTACTAAATATACTATGATGGACTTTTGCTACGCTTTGAGGCATTTTGACCCAAAGGACTGCGAAGTTCTTAAAGAAATACTTGTCACTTATGGTTGTATAGAAGATTATCACCCTAAGTGGTTTGAAGAGAATAAGGATTGGTACGACCCAATAGAAAACCCAAAATATTATGCCATGTTGGCTAAAATGGGGCCTATTGTACGACGTGCTCTATTGAATGCTATTGAGTTCGGAAACCTTATGGTTGAAAAAGGTTATGTTGGTGTTGTTACACTTGATAACCAAGATCTTAACGGTAAATTTTATGATTTTGGTGATTTTCAAAAAACAGCACCTGGTGCTGGTGTTCCTGTTTTTGATACATATTATTCTTACATGATGCCCATCATAGCCATGACGGATGCTTTGGCACCTGAAAGGTATTTTGAATATGATGTGCATAAGGGTTATAAGTCTTATGATCTCCTCAAGTATGATTATACTGAGGAGAAACAAGAGTTGTTTCAGAAATACTTTAAGTATTGGGACCAGGAGTACCATCCTAACTGCCGTGACTGTATTGATGACAGGTGTTTGATACATTGTGCAAACTTCAACATCTTGTTTTCTACACTGATACCGCAGATTTCTTTTGGTAATTTGTGTAGAAAGGTGTTTGTTGATGGTGTACCTTTTATAGCTACTTGTGGCTATCATTCCAAAGAACTTGGTGTTATTATGAATCAAGATAACACTATGTCGTTCTCAAAAATGGGTTTAAGTCAACTCATGCAGTTTGTTGGAGACCCTGCCTTGTTAGTGGGAACATCCAATAATTTAATCGATCTTAGAACGTCTTGTTTTAGTGTTTGTGCATTGGCGTCTGGTATTACTCATCAAACGGTAAAACCAGGTCACTTTAACAAGGATTTCTATGATTTTGCAGAGAAGGCTGGTATGTTTAAGGAAGGTTCTTCTATACCACTTAAACATTTCTTCTACCCTCAGACTGGTAATGCTGCTATAAACGATTATGATTATTATCGTTATAACAGGCCTACCATGTTCGATATACGTCAACTTCTATTTTGTTTAGAAGTGACTTCTAAATACTTTGAATGCTATGAAGGCGGCTGTATACCAGCAAGCCAAGTTGTAGTTAATAATCTAGATAAGAGCGCAGGCTACCCATTTAATAAGTTTGGAAAAGCCCGTCTCTATTATGAAATGAGTCTAGAGGAACAGGACCAACTCTTTGAGAGTACAAAGAAGAATGTCCTGCCCACTATAACTCAAATGAATTTAAAATATGCCATATCCGCGAAAAATAGAGCGCGTACAGTGGCAGGTGTGTCTATCCTTTCTACTATGACTAATAGGCAGTTTCATCAGAAGATTCTTAAGTCTATAGTCAACACTAGAAACGCTCCTGTAGTTATTGGAACAACCAAGTTTTATGGCGGTTGGGACAATATGTTGAGAAACCTTATTCAGGGTGTTGAAGATCCGATTCTTATGGGTTGGGACTATCCAAAGTGTGATAGAGCAATGCCAAATTTGCTACGTATAGCAGCATCTTTGGTACTTGCTCGGAAACACACTAACTGTTGTACTTGGTCTGAGCGCATTTATAGGTTGTATAATGAATGCGCTCAGGTTTTATCAGAAACTGTCCTAGCTACAGGTGGTATTTATGTAAAACCTGGTGGTACTAGCAGTGGTGATGCTACTACTGCTTATGCAAACAGTGTTTTTAATATAATACAAGCTACATCTGCTAATGTTGCGCGTCTTTTGAGTGTTATAACGCGTGATATTGTTTATGATGACATTAAGAGCCTGCAGTATGAGTTGTACCAGCAGGTTTATAGGCGAGTTAATTTTGACCCAGCCTTTGTAGAAAAGTTTTATTCTTACTTATGTAAGAATTTCTCTTTGATGATCTTGTCCGACGACGGTGTTGTTTGTTATAACAATACACTAGCCAAACAAGGTCTTGTAGCAGATATTTCTGGTTTTAGAGAAGTTCTCTACTACCAAAATAATGTCTTTATGTCTGACGCTAAATGTTGGGTGGAACCAGATTTAGAAAAAGGCCCTCATGAATTTTGTTCACAGCATACAATGCTAGTGGAAGTGGATGGTGAGCCTAAATACTTGCCATATCCAGACCCTTCACGCATTTTAGGTGCATGTGTTTTTGTAGATGATGTGGATAAGACGGAACCTGTGGCTGTTATGGAGCGTTATATAGCTCTAGCCATAGACGCTTACCCGCTAGTACATCATGAAAATGAGGAGTACAAGAAGGTGTTCTTTGTGCTTCTTTCATACATCAGAAAACTCTATCAAGAGCTTTCTCAGAATATGCTTATGGACTACTCTTTTGTAATGGATATAGACAAGGGTAGTAAATTTTGGGAACAGGAGTTCTATGAGAATATGTATAGAGCTCCTACGACTTTACAATCTTGTGGTGTCTGTGTAGTTTGTAATAGTCAAACTATACTGCGCTGTGGTAATTGTATTCGCAAACCATTTTTGTGTTGTAAATGTTGCTATGACCATGTCATGCATACAGACCACAAAAATGTTTTGTCTATAAATCCATACATTTGCTCACAGCCCGGTTGTGGCGAGGCAGATGTTACTAAATTGTACCTCGGAGGTATGTCATACTTCTGTGGTAATCATAAACCAAAATTGTCAATACCGTTGGTATCTAATGGTACTGTTTTTGGAATTTACAGGGCTAATTGTGCTGGTAGCGAAAGTGTTGATGATTTTAATCAACTAGCTACTACTAATTGGTCTACTGTGGAACCTTATATTTTGGCAAATCGCTGTAGTGACTCATTGAGACGCTTTGCTGCGGAAACAGTAAAAGCTACAGAGGAGTTGCATAAGCAGCAGTTTGCTAGTGCTGAAGTGCGAGAAGTTCTCTCAGATCGTGAGTTGATTCTATCATGGGAGCCAGGTAAAACTAGGCCTCCATTGAATAGGAATTATGTCTTTACAGGCTATCACTTTACAAGAACTAGTAAGGTGCAGCTTGGTGATTTTACATTTGAAAAAGGTGAAGGTAAAGATGTTGTCTATTATAGGGCAACGTCCACTGCTAAATTGTCTGTTGGAGACATTTTTGTTTTAACTTCACACAATGTTGTTTCTCTTGTAGCACCAACATTGTGTCCACAACAGACCTTTTCTAGGTTTGTAAACTTAAGACCTAATGTAATGGTACCAGAATGTTTTGTGAACAACATTCCACTCTACCATTTAGTAGGTAAGCAGAAGCGTACTACAGTACAAGGTCCCCCAGGCAGTGGTAAATCACATTTTGCTATAGGCCTTGCAGCATACTTTAGTAACGCTCGTGTTGTCTTTACTGCATGTTCTCATGCAGCTGTTGATGCTTTATGTGAAAAAGCTTTTAAGTTTTTAAAAGTTGATGATTGCACTAGGATAGTACCTCAAAGAACTACTATCGACTGCTTTTCAAAGTTTAAAGCTAATGACACAGGCAAAAAGTATATTTTTAGTACTATAAATGCCTTGCCAGAAGTTAGTTGTGACATTCTTTTGGTTGACGAGGTTAGTATGTTGACCAATTATGAATTGTCTTTTATTAATGGTAAGATAAACTACCAATATGTTGTGTATGTAGGTGATCCCGCTCAATTACCGGCACCTCGTACCTTACTTAATGGTTCACTTTCACCAAAGGATTATAATGTTGTAACAAACCTTATGGTTTGCGTTAAACCCGATATCTTCCTTGCGAAGTGTTACCGTTGTCCTAAGGAAATTGTAGACACTGTGTCTACTCTTGTTTATGATGGAAAGTTTATTGCAAATAACCCAGAATCACGTCAGTGTTTCAAGGTTATAGTTAATAATGGCAATTCTGATGTAGGACATGAAAGTGGTTCAGCCTACAACACAACTCAATTAGAATTTGTGAAAGATTTTGTTTGTCGCAATAAGGAGTGGCGGGAAGCAACATTCATTTCACCTTATAATGCTATGAACCAGAGAGCCTATCGTATGCTTGGACTTAATGTTCAGACAGTAGACTCGTCTCAAGGTTCAGAGTATGATTATGTTATATTCTGTGTTACAGCAGATTCGAATCATGCACTGAATATTAACAGATTCAATGTAGCGCTTACAAGAGCTAAGCGTGGTATACTAGTTGTCATGCGTCAGCGTGATGAATTGTATTCGGCTCTTAAGTTTACAGAGCTTGATAGTGAAACAAGTCTGCAAGGTACAGGTTTGTTTAAAATTTGCAACAAGGACTTTAGTGGTGTCCATCCTGCTTATGCAGTCACAACTAAGGCTCTTGCCGCAACTTATAAAGTTAATGATGAACTTGCTGCACTTGTTAATGTGGAAGCTGGTTCAGAAATAACATATAAACATCTTATTTCTCTTTTAGGATTTAAGATGAGTGTTAATGTTGAAGGCTGCCACAACATGTTTATAACACGTGAAGAGGCAATTCGTAATGTGAGAGGTTGGGTAGGTTTTGATGTAGAAGCTACACATGCTTGTGGTACTAACATCGGCACTAACTTGCCTTTTCAAGTAGGTTTCTCTACTGGTGCTGACTTTATAGTCACGCCTGAGGGAATTGTAGATACTTCAATAGGCAATAATTTTGAGCCTGTTAATTCTAAGGCACCTCCAGGTGAACAATTTAATCACTTAAGGGCTTTATTTAAAAGTGCTAAACCTTGGCATGTTATAAGACCAAGGATTGTACAAATGTTAGCAGACAACCTATGCAATGTTTCAGATTGCGTAGTTTTTGTAACTTGGTGTCATGGTCTAGAACTAACTACTTTGCGCTATTTTGTTAAAATAGGCAAAGAACAAGTATGTTCTTGTGGTTCTAGAGCTACAACATTTAATTCTCATACTCAAGCTTATGCTTGTTGGAAGCATTGTTTGGGTTTTGATTTTGTTTATAACCCACTTCTAGTGGATGTTCAACAGTGGGGTTACTCTGGTAACCTACAATTTAATCATGACTTGCACTGTAATGTGCATGGACACGCGCATGTTGCCTCTGCGGATGCTATTATGACGCGTTGTCTTGCAATTAACAATGCATTTTGTCAAGATGTCAACTGGGATTTGACATACCCTCATATTGCAAATGAGGATGAAGTCAATTCTAGTTGTAGATACTTACAACGCATGTATCTTAATGCATGTGTTGATGCTCTTAAAATTAACGTTGTCTATGATATAGGCAACCCTAAAGGTATAAAATGTGTTAGACGTGGAGACTTGAGTTTTAGATTCTATGATAAGAATCCAATAGTACCCAACGTCAAGCAGTTTGAGTATGACTATAATCAGCATAAAGATAAGTTTGCTGATGGTCTTTGTATGTTCTGGAATTGTAATGTGGATTGTTATCCTGATAATTCCTTGGTTTGCAGGTATGACACACGAAATTTGAGTGTGTTTAACTTACCAGGTTGTAATGGTGGTAGCCTGTATGTCAATAAACATGCATTCCACACACCTAAATTTGATCGCATTAGCTTTCGTAATTTGAAAGCTATGCCATTCTTTTTCTATGACTCATCTCCTTGCGAAACCATTCAAGTGGATGGAGTTGCACAGGATCTTGTGTCACTAGCTACTAAAGATTGTATCACAAAATGCAACATAGGCGGTGCTGTTTGTAAGAAACATGCGCAGATGTATGCAGAGTTTGTGACTTCTTATAATGCAGCGGTAACAGCTGGTTTTACTTTTTGGGTTACTAATAATTTTAACCCATATAATTTGTGGAAAAGTTTTTCAGCTCTCCAGTCTATCGATAACATTGCTTATAATATGTATAAGGGTGGTCATTACGACGCTATTGCAGGAGAAATACCCACCATCGTAACTGGAGATAAAGTTTTTGTTATTGATCAAGGTGTAGAAAAGGCAGTTTTTGTTAATCAAACAACACTGCCTACTTCTGTGGCGTTTGAACTGTATGCGAAGAGAAATATTCGCACACTGCCAAACAACCGTATTTTGAAGGGTCTTGGTGTAGATGTAACCAATGGTTTTGTAATTTGGGATTATGCGAACCAAACACCATTATATCGTAATACTGTTAAGGTATGTGCATACACAGACATTGAGCCAAATGGCCTAATAGTTCTGTATGATGATAGATATGGTGATTACCAATCTTTTCTTGCCGCTGATAATGCTGTTCTAGTTTCTACACAGTGTTATAAGCGATATTCATATGTAGAAATACCGTCAAACATGCTTGTTCAGAATGGTATGCCATTAAAAGACGGAGCGAATCTGTATGTCTATAAGCGCGTTAATGGAGCGTTTGTTACGCTACCTAACACACTAAACACACAAGGTCGCAGTTATGAAACTTTTGAACCTCGTAGCGACGTTGAGCGTGATTTTCTCGACATGTCGGAAGAGGATTTTGTAGAAAAGTATGGTAAAGACTTAGGTCTACAACACATACTGTATGGTGAAGTTGATAAACCACAATTGGGCGGTTTGCACACTGTTATAGGTATGTACAGACTTTTACGTGCGAATAAGTTGAATGCAAAGTCTGTTACTAATTCAGATTCTGATGTCATGCAAAATTATTTTGTGTTGGCAGATAATGGTTCTTACAAGCAAGTGTGCACTGTTGTGGATTTACTGCTTGATGATTTCTTAGAACTGCTTAGGAACATACTGAATGAGTATGGTACTAATAAGTCAAAAGTTGTAACAGTGTTAATTGATTACCATAGCATAAATTTTATGACTTGGTTTGAAGATGGCAGTATTAAAACATGTTATCCACAGCTTCAATCAGCATGGACGTGTGGTTATAATATGCCTGAACTCTATAAAGTCCAGAATTGTGTTATGGAACCTTGCAACATTCCTAATTATGGTGTTGGAATAACGTTGCCAAGTGGTATTATGATGAATGTGGCAAAGTACACACAACTTTGTCAATACCTTTCGAAAACAACAATGTGTGTGCCGCATAATATGCGCGTTATGCATTTTGGAGCTGGCAGTGATAAAGGAGTGGCTCCAGGTAGTACTGTTCTTAAACAGTGGCTTCCTGAAGGGACACTCCTTGTAGATAATGATATTGTAGATTATGTGTCTGATGCACATGTTTCTGTGCTTTCAGATTGCAATAAATATAAGACAGAGCACAAGTTTGATCTTGTGATATCTGATATGTATACAGACAATGATTCAAAAAGAAAGCATGAAGGCGTGATAGCCAACAATGGCAATGATGACGTTTTCATATATCTTTCAGACTTTCTTCGTAACAATTTGGCTCTTGGCGGCAGTTTTGCTGTAAAGGTGACAGAGACAAGTTGGCACGAGAATTTATATGACATTGCACAAGATTGTGCATGGTGGACAATGTTTTGTACTGCAGTGAATGCTTCTTCTTCAGAAGCATTTCTGGTTGGTGTTAATTATTTGGGTGCAAGTGAAAAGCTTAAAGTTAATGGAAAAACCCTGCACGCAAATTATATATTTTGGAGGAATTGTAATTATTTACAAACCTCAGCTTATAGTATATTTGACGTTGCTAAGTTTGATTTGAAATTAAAAGCAACGCCAGTTGTAAATTTGAAAACTGAACAAAAGACCGACTTAGTAGTTAATTTACTAAGGAACGGTAAATTGTTAGTTAGAGATGTTGGTGAAGTCACTGTTTCTAGTGACCATTTTGTTTGCACTATGTAGTGCTAATTTATATGACAACGAATCTTTTGTGTATTACTACCAGAGTGCTTTTAGGCCAGGACATGGTTGGCATTTACATGGAGGTGCTTATGCAGTAGTTAATGTGTCTAGTGAAAATAATAATGCAGGTACTGCCCCAAGTTGCACTGCTGGTGCTATTGGCTACAGTAAGAATCTCAGTGCGGCCTCAGTAGCCATGACTGCACCACTAAGTGGTATGTCATGGTCTGTCAACTCTTTTTGTACAGCCCACTGTAATTTTACTTCTTATATAGTGTTTGTTACACATTGTTATAAGAGCGGATCTAATAGTTGTCCTTTGACAGGTCTTATTCCAAGCGGTTATATTCGTATTGCTGCTATGAAACATGGAAGTGCTATGCCTGGTCACTTATTTTATAATTTAACAGTTTCTGTGACTAAATATCCTAAGTTTAGATCGCTACAATGTGTTAATAATTATACTTCTGTATATTTAAATGGTGACCTTGTTTTCACATCTAACTATACTGAAGATGTTGTAGCTGCAGGTGTCCATTTTAAAAGTGGTGGACCTATAACTTATAAAGTTATGAGAGAGGTTAAAGCCTTGGCTTATTTTGTCAATGGTACTGCACATGATGTCATTCTATGTGATGACACACCTAGAGGTTTGTTAGCATGCCAATATAATACTGGCAATTTTTCAGATGGCTTCTATCCTTTTACTAATACTAGTATTGTTAAGGATAAGTTTATTGTTTATCGTGAAAGTAGTGTCAATACTACTTTAACATTAACTAATTTCACGTTTAGTAATGAAAGTGGTGCCCCTCCTAATACAGGTGGTGTTGACAGTTTTATTTTATACCAGACACAAACAGCTCAGAGTGGTTATTATAATTTTAACTTTTCATTTCTGAGTAGTTTTGTTTATAGGGAAAGTAATTATATGTATGGATCTTACCATCCACGTTGTAGTTTTAGACCTGAAACTCTTAACGGTTTGTGGTTTAATTCCCTTTCTGTTTCATTAACATACGGTCCCATTCAAGGTGGTTGTAAGCAATCTGTATTTAATGGTAAAGCAACTTGTTGTTATGCTTATTCATACGGAGGACCTCGTGGTTGTAAAGGTGTCTATAGAGGTGAGCTAACACAGCATTTTGAATGTGGTTTGTTAGTTTATGTTACTAAGAGCGATGGCTCCCGTATACAAACTGCAACACAACCACCTGTATTAACCCAAAATTTTTATAATAACATCAATTTAGGTAAGTGTGTTGATTATAATATATATGGCAGAATTGGCCAAGGTCTTATTACTAATGTAACCGACTTAGCTGTTAGTTATAATTATTTATCAGACGCAGGTTTGGCTATTTTAGATACATCTGGTGCCATAGACATCTTCGTTGTACAAGGTGAATATGGTCCTAACTATTATAAGGTTAATCCATGTGAAGATGTCAACCAACAGTTTGTAGTTTCTGGTGGTAAATTAGTAGGTATTCTCACTTCACGTAATGAAACAGGTTCTCAGCTTCTTGAGAACCAGTTTTATATTAAAATCACTAATGGAACTCGTCGTTCTAGACGTTCTGTTACTGAAAATGTTACAAATTGCCCTTATGTTAGTTATGGCAAGTTTTGTATAAAACCTGATGGTTCAATTTCTGTAATAGTACCAAAAGAACTGGATCAGTTTGTGGCACCTTTACTTAATGTTACTGAATATGTGCTCATACCTAACAGTTTTAATTTAACTGTTACAGATGAGTACATACAAACGCGTATGGATAAGATCCAAATTAATTGCCTGCAGTATGTTTGTGGCAATTCTTTGGCCTGTAGAAAGCTGTTTCAACAATATGGGCCTGTTTGTGACAACGTATTGTCTGTAGTAAATAGTGTTGGTCAAAAAGAAGATATGGAACTTTTAAATTTCTATTCTTCTACTAAACCAGCTCGTTTTAATACACCAGTTTTTAGTAATCTTAGCACTGGTGAGTTTAATATTTCTCTTTTGTTAACATCCCCTAGTAGTCCTAGGAGGCGTTCTTTTATTGAAGATCTTTTATTTACAAGTGTTGAATCTTTAGGATTACCAACAGATGACGCATACAAAAAGTGCACTGCAGGACCTTTAGGCTTTTTTAAAGACCTTGCATGTGCTCGTGAATATAATGGTTTGCTTGTGTTGCCTCCTATTATAACAGCAGAAATGCAAACTTTGTATACTAGTTCTTTAGTAGCTTCTATGGCTTTTGGTGGTATTACTGCAGCTGGTGCCATACCTTTTGCCACACAACTGCAGGCTAGAATTAATCACTTGGGTATTACCCAGTCACTTTTGTTGAAGAATCAAGAAAAAATTGCTGCTTCCTTTAATAAGGCCATTGGTCATATGCAGGAAGGTTTTAGGAGTACATCTCTAGCATTACAACAAATTCAAGATGTTGTTAATAAGCAGAGTGCTATTCTTACTGAGACTATGGCAGCACTTAATAAAAATTTTGGTGCTATTTCTTCTGTGATTCAAGACATTTACCAGCAACTTGATTCCATACAAGCAGATGCTCAAGTGGATCGGCTCATAACTGGTAGATTGTCATCACTTTCTGTCTTAGCATCTGCTAAGCAGTCGGAGTACATTAGAGTGTCACAACAGCGTGAGTTAGCTACTCAGAAAATTAATGAGTGTGTTAAATCACAGTCTATTAGGTATTCCTTTTGTGGTAATGGACGACATGTTTTAACCATACCACAAAATGCCCCTAATGGTATAGTGTTTATACACTTTACTTATACACCAGAGAGCTTTATTAATGTTACTGCAATAGTGGGTTTTTGTGTAAGTCCTGCTAATGCTAGTCAGTATGCAATAGTGCCCGCTAATGGTAGGGGTATTTTTATACAAGTTAATGGTAGTTACTACATCACTGCACGAGATATGTATATGCCAAGAGATATTACTGCAGGAGATATAGTTACGCTTACTTCTTGTCAAGCAAATTATGTAAGTGTAAATAAGACCGTCATTACTACATTTGTAGACAATGATGATTTTGATTTTGATGATGAATTGTCAAAATGGTGGAATGATACTAAGCATGAGCTACCAGACTTTGACAAATTCAATTACACAGTACCTATACTTGACATTGATAGTGAAATTGATCGTATTCAAGGCGTTATACAGGGTCTTAACGACTCTCTAATAGACCTTGAAACACTATCAATACTCAAAACTTATATTAAGTGGCCTTGGTATGTGTGGTTAGCCATAGCTTTTGCCACTATTATCTTCATCTTAATACTAGGATGGTTGTTTTTCATGACTGGTTGTTGTGGTTGTTGTTGTGGATGCTTTGGCATTATTCCTTTAATGAGTAAGTGTGGTAAGAAATCTTCTTATTACACGACTTTTGATAATGATGTGGTAACTGAACAATACAGACCTAAAAAGTCTGTTTAATGATTCAAAGTCCCACATCTTTTCTAATAGTATTAATTTTTCTTTGGTGTAAACTTGCACTAAGTTGTTTTAAAGAGTGTGTTATAGCACTCCAGCAACTAATACAAGTTTTACTCCAAATTATTAATAGTAACTTACAGTCTAGACTTCTGCTTTGGCACAGTCTAGACTAATGTTAGATTTTGAAGCAATTATTGAAACTGGTCAGCAAATAATTCAACAAATCAGTTTCGATTTACAGCAAATTTCAAGTGTGCTAAGCACTGAATTATTTGACCCCTTTGAAGTCTGTGTTTACAGAGGAGGTAATTATTGGGAGTTAGAGTCAGCTGACGAGTTTTCAGGTGATGACGAATATATTGAGTAAATCGCTAGAGGAGAACGGAAGTTTCCTAACAGCAGTTTACATATTTGTTGGATTTTTAGTATTTTACCTATTAGGTAGAGCACTCCAAGCATTTGTACAAGCTGCTGATGCTTGTTGTTTATTTTGGTATACATGGGTAGTAGTTCCTGGAGCTAAGGGTACAGCCTTTGTGTATAATCATACATATGGTAAAAAACTTAACAAACCGGAGTTAGAAGCGGTTATTGTTAACGAGTTCCCCAAGAACGGTTGGAATAATAAAAGTCCAGCAAATTTCCAATATGATGGAAAATTGCACACTTAACTTAGAGCAGGCAACTCTGCTTTTTAAAGAATATAATTTATTTATAACCGCATTCCTATTGTTTCTTACTATACTACTTCAGTATGGGTACGCAACTAGGAGTCGGTTTATTTATATACTGAAAATGATAGTGTTATGGTGCTTTTGGCCCCTTAACATTGCAGTAGGTGTAATTTCATGTATATATCCACCAAATACAGGAGGTCTTGTCGCAGCGATAATACTTACTGTGTTTGCTTGTCTTTCTTTTGTAGGTTATTGGATTCAGAGTTGTAGACTCTTTAAAAGGTGTAGGTCTTGGTGGTCTTTTAACCCCGAGTCTAATGCCGTAGGTTCAATACTCCTCACAAATGGTCAACAATGTAATTTTGCTATAGAGAGTGTGCCTATGGTGCTTGCTCCAATTATAAAGAACGGTGTCCTTTATTGTGAGGGTCAGTGGCTTGCTAAATGTGAACCAGACCACTTGCCTAAAGACATATTTGTATGCACACCGGATAGACGTAATATCTATCGTATGGTGCAGAAATACACTGGTGACCAAAGCGGAAATAAGAAAAGGTTTGCTACATTTGTCTATGCAAAGCAGTCAGTAGACACTGGCGAGCTAGAAAGTGTAGCAACAGGAGGAAGTAGTCTTTACACATAAATGTGTGTGTGTAGAGAGTATTTAAGACTATTCTTTAATAGTGCCTCTATTTTAAGAGCGCATACGAGTATTTATTTTGAGGATATTAATATAAATCCTCTTTGTTTTATACTCTCTTTTCAAGAGCTATTATTTAAAAAACAGTTTTTCCACTCTTTTGTGCCAAAAACTATTGTTGTTAACGGTGTTACCTTTCAAGTGGATAATGGAAAAGTCTACTACGAAGGAACACCAGTTTTCCAAAAAGGTTGTTGTAGAATGTGGTCCAATTATAAGAAAGATTAGAATAATTAAGCCACCAACTACACTTATTTTTATAAGAGGCGTTTTATCTTACAAACGCTTAACAAATACGGACGATGAAATGGCTGACTAGTTTTGGAAGAGCAGTTATTTCATGTTATAAAGCCCTACTATTAACTCAATTAAGAGTGTTAGATAGGTTAATTTTAGGTCACGGACCAAAACGCGTTTTAACGTGTAGTAGGCGAGTGCTTTTGTTTCAGTTAGATTTAGTTTATAGGTTGGCGTTTACGCCCACCCAATCGCTGGTATGAATAATAGTAAAGATAATCCTTTTCGCGGAGCAATAGCAAGAAAAGCGCGAGTTTATCTGAGAGAAGGATTAGATTGTGTTTACTTTCTTAACAAAGCAGGACAAGCAGAGCCTTGTCCCGCGTGTACCTCTCTAGTATTCCAAGGGAAAACTTGTGAGGCACACATAAATAATAATAATCTTTTGTCATGGCAAGCGGTAAGGCAACTGGAAAGACAGACGCCCCAGCGCCAGTCATCAAACTAGGAGGACCAAAGCCACCTAAAGTTGGTTCTTCTGGAAATGCATCTTGGTTTCAAGCAATAAAAGCCAAGAAGCTAAATTCACCTCCACCTAAGTTTGAAGGTAGCGGTGTTCCTGATAATGAAAATCTTAAAATAAGCCAGCAACATGGATACTGGAGACGCCAAGCTAGGTTTAAGCCAGGTAAAGGCGGAAGAAAACCAGTCCCAGATGCTTGGTACTTCTATTATACTGGAACAGGACCAGCCGCTGACCTGAATTGGGGTGATAGCCAAGATGGTATAGTGTGGGTTGCTGCAAAGGGTGCTGATGTTAAATCTAGATCTAACCAGGGTACAAGGGACCCTGACAAGTTTGACCAATATCCACTACGATTCTCGGACGGAGGACCTGATGGTAATTTCCGTTGGGACTTCATTCCTCTGAATCGTGGTAGGAGTGGAAGATCAACAGCAGCTTCATCAGCAGCATCTAGTAGAGCACCGTCGCGTGACGGCTCGCGTGGTCGTAGAAGTGGTTCTGAAGATGATCTTATTGCTCGTGCAGCAAAGATAATCCAGGATCAGCAGAAGAAGGGTTCTCGCATTACTAAGGCTAAGGCTGATGAAATGGCTCATCGCCGGTATTGCAAGCGCATTATTCCACCTGGTTATAAGGTTGATCAAGTCTTTGGTCCCCGTACTAAAGGTAAGGAGGGAAATTTTGGTGATGACAAGATGAATGAGGAAGGTATTAAGGATGGGCGTGTTACGGCAATGCTCAACCTAGTCCCTAGCAGCCATGCTTGCCTTTTTGGAAGTAGGGTGACGCCCAAACTTCAACCAGATGGGCTTCACTTGAGATTTGAATTTACTACTGTGGTCCCGCGTGATGATCCGCAGTTTGATAATTATGTGAAAATTTGTGACCAGTGTGTTGATGGTGTAGGAACACGTCCAAAAGATGACGAACCGAGACCAAAGTCACGCTCAAGTTCAAGACCTGCTACAAGAACAAGTTCTCCGGCGCCAAGACAACAACGCCCAAAGAAGGAGAAAAAGTCAAAGAAGCAGGATGATGAAGTAGATAAAGCATTGACCTCAGATGAGGAGAGGAACAATGCACAGCTGGAATTTGATGATGAACCCAAGGTTATTAACTGGGGGGATTCAGCTTTAGGTGAGAATGAACTTTGAGTAACATAATGGACCTGCTGCATTTTTTGGTACATTTTGTTAAACACTATTTCTGTGCTTTCCTATCAATTATTACAGGCATTGATTGTGATTATGTGCAATATTTAAGCTTCTTTTGGTTGCTTTTTGCTTGTTGTGTTGTTGCTGTGCTTTTTATTATTGTGATTCTCATTAGTTTGTTTTATCGTAGAAGTTCAATAGTAAGAGTTAAGGAAGATAGGCATGTAGCTTAGCACCTACATGTCTATCGCCAGGGAAATGTCTAATCTGTCTACTTAGTAGCCTGGAAACGAACGGTAGACCCTTAGATTTTAATTTAGTTTAATTTTTAGTTTAGTTTAAGTTAGTTTAGAGTAGGTATAAAGATGCCAGTGCCGGGGCCACGCGGAGTACGATCGAGGGTACAGCACTAGGACGCCCATTAAGGGAAGAGCTAAATTTTAGTTTAAGTTAAGTTTAATTGGCTAAGTATAGTTAAAATTTGTAGGCTAGTATAGAGTTAGAGCAAAAAAAAAAAAAA p0c6v3-pro_0000338322 Non-structural protein 9 ORF1a polyprotein 1a protein protein ibvb ibvb IBV NNELMPHGVKTKACVAGVDQAHCSVESKCYYTNISGNSVVAAITSSNPNLKVASFLNEAGNQIYVDLDPPCKFGMKVGVKVEVVYLYFIKNTRSIVRGMVLGAISNVVVLQ 3673 3783 aibv_rna_3'utr Avian infectious bronchitis virus-ssrna-3'UTR rna ribonucleic acid RNA rna aibv Avian infectious bronchitis virus-Beaudette strain TGAGTAACATAATGGACCTGTTGTTTCCTGGTACATTTTGTTAAACACTATTTCTGTGCTTTCCTATCAATTATTACAGGCATTGATTGTGATTATGTTCAATACTTAAGCTTCTTTTGGTTGCTTTTTGCTTATTGTATTGTTGCTGTGCTTTTTATTGTTGTGATTCTCATTAGTTTGCTTTATCGTAGAAATTCAATAGTAAGAGTTAAGGAAGATAGGCATGTAGCTTGATTACCTACATGTCTATCGCCAGGGAAATGTCTAATCTGTCTACTTAGTAGCCTGGAAACGAACGGTAGACCCTTAGATTTTAATTTAGTTTAATTTTTAGTTTAGTTTAAGTTAGTTTAGAGTAGGTATAAAGATGCCAGTGCCGGGGCCACGCGGAGTACGATCGAGGGTACAGCACTAGGACGCCCATTAGGGGAAGAGCTAAATTTTAGTTTAAGTTAAGTTTAATTGGCTAAGTATAGTTAAAATTTATAGGCTAGTATAGAGTTAGAGCA aibv_rna_antisense_3'utr Avian infectious bronchitis virus-ssrna-antisense-3'UTR rna ribonucleic acid RNA rna aibv Avian infectious bronchitis virus-Beaudette strain TGCTCTAACTCTATACTAGCCTATAAATTTTAACTATACTTAGCCAATTAAACTTAACTTAAACTAAAATTTAGCTCTTCCCCTAATGGGCGTCCTAGTGCTGTACCCTCGATCGTACTCCGCGTGGCCCCGGCACTGGCATCTTTATACCTACTCTAAACTAACTTAAACTAAACTAAAAATTAAACTAAATTAAAATCTAAGGGTCTACCGTTCGTTTCCAGGCTACTAAGTAGACAGATTAGACATTTCCCTGGCGATAGACATGTAGGTAATCAAGCTACATGCCTATCTTCCTTAACTCTTACTATTGAATTTCTACGATAAAGCAAACTAATGAGAATCACAACAATAAAAAGCACAGCAACAATACAATAAGCAAAAAGCAACCAAAAGAAGCTTAAGTATTGAACATAATCACAATCAATGCCTGTAATAATTGATAGGAAAGCACAGAAATAGTGTTTAACAAAATGTACCAGGAAACAACAGGTCCATTATGTTACTCA eif3f_human Eukaryotic translation initiation factor 3 subunit F Eukaryotic translation initiation factor 3 subunit 5 eIF-3-epsilon eIF3 p47 EIF3F EIF3S5 Deubiquitinating enzyme eIF3f protein protein human Homo sapiens Human MATPAVPVSAPPATPTPVPAAAPASVPAPTPAPAAAPVPAAAPASSSDPAAAAAATAAPGQTPASAQAPAQTPAPALPGPALPGPFPGGRVVRLHPVILASIVDSYERRNEGAARVIGTLLGTVDKHSVEVTNCFSVPHNESEDEVAVDMEFAKNMYELHKKVSPNELILGWYATGHDITEHSVLIHEYYSREAPNPIHLTVDTSLQNGRMSIKAYVSTLMGVPGRTMGVMFTPLTVKYAYYDTERIGVDLIMKTCFSPNRVIGLSSDLQQVGGASARIQDALSTVLQYAEDVLSGKVSADNTVGRFLMSLVNQVPKIVPDDFETMLNSNINDLLMVTYLANLTQSQIALNEKLVNL spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT spike_ibvb Spike glycoprotein S E2 Peplomer protein 2 protein protein ibvb ibvb IBV MLVTPLLLVTLLCALCSAVLYDSSSYVYYYQSAFRPPSGWHLQGGAYAVVNISSEFNNAGSSSGCTVGIIHGGRVVNASSIAMTAPSSGMAWSSSQFCTAHCNFSDTTVFVTHCYKHGGCPLTGMLQQNLIRVSAMKNGQLFYNLTVSVAKYPTFRSFQCVNNLTSVYLNGDLVYTSNETIDVTSAGVYFKAGGPITYKVMREVKALAYFVNGTAQDVILCDGSPRGLLACQYNTGNFSDGFYPFTNSSLVKQKFIVYRENSVNTTCTLHNFIFHNETGANPNPSGVQNIQTYQTKTAQSGYYNFNFSFLSSFVYKESNFMYGSYHPSCKFRLETINNGLWFNSLSVSIAYGPLQGGCKQSVFKGRATCCYAYSYGGPSLCKGVYSGELDHNFECGLLVYVTKSGGSRIQTATEPPVITQNNYNNITLNTCVDYNIYGRTGQGFITNVTDSAVSYNYLADAGLAILDTSGSIDIFVVQGEYGLNYYKVNPCEDVNQQFVVSGGKLVGILTSRNETGSQLLENQFYIKITNGTRRFRRSITENVANCPYVSYGKFCIKPDGSIATIVPKQLEQFVAPLFNVTENVLIPNSFNLTVTDEYIQTRMDKVQINCLQYVCGSSLDCRKLFQQYGPVCDNILSVVNSVGQKEDMELLNFYSSTKPAGFNTPVLSNVSTGEFNISLLLTNPSSRRKRSLIEDLLFTSVESVGLPTNDAYKNCTAGPLGFFKDLACAREYNGLLVLPPIITAEMQALYTSSLVASMAFGGITAAGAIPFATQLQARINHLGITQSLLLKNQEKIAASFNKAIGHMQEGFRSTSLALQQIQDVVSKQSAILTETMASLNKNFGAISSVIQEIYQQFDAIQANAQVDRLITGRLSSLSVLASAKQAEYIRVSQQRELATQKINECVKSQSIRYSFCGNGRHVLTIPQNAPNGIVFIHFSYTPDSFVNVTAIVGFCVKPANASQYAIVPANGRGIFIQVNGSYYITARDMYMPRAITAGDVVTLTSCQANYVSVNKTVITTFVDNDDFDFNDELSKWWNDTKHELPDFDKFNYTVPILDIDSEIDRIQGVIQGLNDSLIDLEKLSILKTYIKWPWYVWLAIAFATIIFILILGWVFFMTGCCGCCCGCFGIMPLMSKCGKKSSYYTTFDNDVVTEQYRPKKSV eif3f_chlae_protein EIF3F Green Monkey protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to O00303 ddx1_human ATP-dependent RNA helicase DDX1 DDX1 DEAD box protein 1 DEAD box protein retinoblastoma protein protein human Homo sapiens Human MAAFSEMGVMPEIAQAVEEMDWLLPTDIQAESIPLILGGGDVLMAAETGSGKTGAFSIPVIQIVYETLKDQQEGKKGKTTIKTGASVLNKWQMNPYDRGSAFAIGSDGLCCQSREVKEWHGCRATKGLMKGKHYYEVSCHDQGLCRVGWSTMQASLDLGTDKFGFGFGGTGKKSHNKQFDNYGEEFTMHDTIGCYLDIDKGHVKFSKNGKDLGLAFEIPPHMKNQALFPACVLKNAELKFNFGEEEFKFPPKDGFVALSKAPDGYIVKSQHSGNAQVTQTKFLPNAPKALIVEPSRELAEQTLNNIKQFKKYIDNPKLRELLIIGGVAARDQLSVLENGVDIVVGTPGRLDDLVSTGKLNLSQVRFLVLDEADGLLSQGYSDFINRMHNQIPQVTSDGKRLQVIVCSATLHSFDVKKLSEKIMHFPTWVDLKGEDSVPDTVHHVVVPVNPKTDRLWERLGKSHIRTDDVHAKDNTRPGANSPEMWSEAIKILKGEYAVRAIKEHKMDQAIIFCRTKIDCDNLEQYFIQQGGGPDKKGHQFSCVCLHGDRKPHERKQNLERFKKGDVRFLICTDVAARGIDIHGVPYVINVTLPDEKQNYVHRIGRVGRAERMGLAISLVATEKEKVWYHVCSSRGKGCYNTRLKEDGGCTIWYNEMQLLSEIEEHLNCTISQVEPDIKVPVDEFDGKVTYGQKRAAGGGSYKGHVDILAPTVQELAALEKEAQTSFLHLGYLPNQLFRTF p0c6y1-pro_0000037415 Proofreading exoribonuclease rep ORF1ab polyprotein 1a-1b protein protein ibvb ibvb IBV GTGLFKICNKEFSGVHPAYAVTTKALAATYKVNDELAALVNVEAGSEITYKHLISLLGFKMSVNVEGCHNMFITRDEAIRNVRGWVGFDVEATHACGTNIGTNLPFQVGFSTGADFVVTPEGLVDTSIGNNFEPVNSKAPPGEQFNHLRVLFKSAKPWHVIRPRIVQMLADNLCNVSDCVVFVTWCHGLELTTLRYFVKIGKEQVCSCGSRATTFNSHTQAYACWKHCLGFDFVYNPLLVDIQQWGYSGNLQFNHDLHCNVHGHAHVASVDAIMTRCLAINNAFCQDVNWDLTYPHIANEDEVNSSCRYLQRMYLNACVDALKVNVVYDIGNPKGIKCVRRGDVNFRFYDKNPIVRNVKQFEYDYNQHKDKFADGLCMFWNCNVDCYPDNSLVCRYDTRNLSVFNLPGCNGGSLYVNKHAFYTPKFDRISFRNLKAMPFFFYDSSPCETIQVDGVAQDLVSLATKDCITKCNIGGAVCKKHAQMYAEFVTSYNAAVTAGFTFWVTNKLNPYNLWKSFSALQ 5469 5989 ddx1_chlae protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to Q92499 ssrna_sars_cov_1-zcrb1-2 3709914 N/A 3709916 predetermined predetermined participant predetermined unspecified role unspecified role bait bait engineered engineered purified purified 5'UTR sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3709917 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. physical association physical association false false false Fig 1 A, Fig 8 A rna_ibv-zcrb1-1 3709914 N/A 3709918 predetermined predetermined participant predetermined unspecified role unspecified role bait bait tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false r.25_26delinsag mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false r.11_12delinsct mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 3709917 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8TBF4:p.Tyr13Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false Q8TBF4:p.[Val53Ala;Phe55Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q8TBF4:p.[Tyr13Ala;Val53Ala;Phe55Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. physical association physical association false false false Fig 1 A, Fig 3, Fig 4 Fig 8 A, Fig 4 rna_ibv-zcrb1-2 3709914 N/A 3709918 predetermined predetermined participant predetermined unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3709917 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. N/A 3709918 competitor competitor competitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 1 B not biotinilated rna_ibv acts as competitor rna_ibv-zcrb1-5 3709914 N/A 3709918 predetermined predetermined participant predetermined unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3709917 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. N/A 3709918 competitor competitor competitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig 1 B not biotinilated rna_ibv acts as competitor rna_ibv-zcrb1-6 3709914 N/A 3709918 predetermined predetermined participant predetermined unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 3709917 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. physical association physical association false false false Fig 1 B not biotinilated rna_ibv acts as competitor s-eif3f-2 3681502 N/A 3681508 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3681509 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 1A eif3f-s-3 3681502 N/A 3681508 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3681510 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind range range range range false myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 4B eif3f-s-1 3681503 N/A 3681508 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3681509 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Fig. 1B s-eif3f-4 3681503 N/A 3681508 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3681510 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Fig. 4A eif3f-s-n-1 3681507 N/A 3681510 unspecified role unspecified role prey prey infection infection N/A 3681511 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false Fig. 4D paps-pppac20_rna_substrate-2 3725490 N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3725493 enzyme enzyme neutral component neutral component in vitro In vitro methylation methylation reaction methylation false false false 1B-G, 3, 4 Positive control experiment; no information is given with respect to purification, expression or construct details for VP39. paps-pppac20_rna_substrate-3 3725490 N/A 3725494 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725495 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P0C6Y5-PRO_0000037395:p.Lys93Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0C6Y5-PRO_0000037395:p.Phe96Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 1B, 2, 3C, 4A, 5A TGEV (Porcine transmissible gastroenteritis coronavirus ) nsp10+16 proteins. paps-pppac20_rna_substrate-1 3725490 N/A 3725496 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3725497 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q98VG9-PRO_5000140217:p.Lys93Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false Q98VG9-PRO_5000140217:p.Phe96Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false 1C, number 1, 2, 3C, 4B, 5A FCoV (feline coronavirus) nsp10+16 proteins. pppac20_rna_substrate-rep-1 3725490 N/A 3725496 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 1C, number 2 FCoV (feline coronavirus) 16 protein. FCoV nsp16 alone showed a low but detectable MTase activity. pppac20_rna_substrate-rep-2 3725490 N/A 3725498 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725499 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P0C6Y0-PRO_0000037363:p.Arg93Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0C6Y0-PRO_0000037363:p.Phe96Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 1D, 3D, 4C, 5A MHV (mouse hepatitis virus) nsp10+16 proteins. pppac20_rna_substrate-rep-3 3725490 N/A 3725500 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725501 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P0C6X7-PRO_0000037317:p.Tyr96Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0C6X7-PRO_0000037317:p.Lys93Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 1E, 2, 3D, 4D, 5A SARS-CoV nsp10+16 proteins. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-4 3725490 N/A 3725502 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725503 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false K9N7C7-PRO_0000422448:p.Lys93Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false K9N7C7-PRO_0000422448:p.Phe96Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 1F, 3D, 4E, 5A MERS-CoV nsp10+16 proteins. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-5 3725490 N/A 3725504 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725505 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P0C6Y1-PRO_0000037412:p.Lys98Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0C6Y1-PRO_0000037412:p.Phe101Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 1G, 2, 4F, 5A IBV (infectious bronchitis virus) nsp10+16 proteins. Identity of "IBV" confirmed from Ref.28 (PMID: 22022266) , Supp. figure 2 sequence. The nsp16 of IBV (Gammacoronavirus) was activated and stimulated y only by its cognate nsp10. Current Uniprot P0C6Y1 sequence numbering for IBV nsp10 differs from that given in figure 3A. pppac20_rna_substrate-rep-13 3725490 N/A 3725496 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3725495 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false 3C, 4B FCoV (feline coronavirus) nsp16 protein and TGEV (Porcine transmissible gastroenteritis coronavirus ) nsp10 protein. FCoV nsp16 could be stimulated to a similar level either by cognate nsp10 or noncognate nsp10s. pppac20_rna_substrate-rep-14 3725490 N/A 3725494 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725497 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 3C, 4A TGEV (Porcine transmissible gastroenteritis coronavirus ) nsp16 protein and FCov nsp10 protein. nsp16 of TGEV could be fully stimulated by the nsp10 of Alphacoronaviruses (FCov). pppac20_rna_substrate-rep-15 3725490 N/A 3725498 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725501 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 3D, 4C MHV (mouse hepatitis virus) 16 protein and SarsCoV nsp10 protein. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-16 3725490 N/A 3725498 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725503 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 3D, 4C MHV (mouse hepatitis virus) 16 protein and MersCoV nsp10 protein. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-17 3725490 N/A 3725502 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725501 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 3D, 4E MERS-CoV 16 protein and SarsCoV nsp10 protein. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-18 3725490 N/A 3725502 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725499 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 3D, 4E MERS-CoV 16 protein and MHV nsp10 protein. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-19 3725490 N/A 3725494 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725499 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4A Weak interaction;TGEV (Porcine transmissible gastroenteritis coronavirus) nsp16 protein and MHV nsp10 protein. nsp16 of TGEV could be fully stimulated by the nsp10 of Alphacoronavirus but less efficiently activated when using the nsp10 of Betacoronavirus and Gammacoronavirus. pppac20_rna_substrate-rep-20 3725490 N/A 3725494 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725501 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4A Weak interaction;TGEV (Porcine transmissible gastroenteritis coronavirus) nsp16 protein and SarsCoV nsp10 protein. nsp16 of TGEV could be fully stimulated by the nsp10 of Alphacoronavirus but less efficiently activated when using the nsp10 of Betacoronavirus and Gammacoronavirus. pppac20_rna_substrate-rep-21 3725490 N/A 3725494 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725505 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4A Weak interaction;TGEV (Porcine transmissible gastroenteritis coronavirus) nsp16 protein and IBV nsp10 protein. nsp16 of TGEV could be fully stimulated by the nsp10 of Alphacoronavirus but less efficiently activated when using the nsp10 of Betacoronavirus and Gammacoronavirus. pppac20_rna_substrate-rep-22 3725490 N/A 3725494 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725503 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4A Weak interaction;TGEV (Porcine transmissible gastroenteritis coronavirus) nsp16 protein and Mers-CoV nsp10 protein. nsp16 of TGEV could be fully stimulated by the nsp10 of Alphacoronavirus but less efficiently activated when using the nsp10 of Betacoronavirus and Gammacoronavirus. pppac20_rna_substrate-rep-23 3725490 N/A 3725496 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3725499 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false 4B FCoV (feline coronavirus) nsp16 protein and MHV nsp10 protein. FCoV nsp16 could be stimulated to a similar level either by cognate nsp10 or noncognate nsp10s. pppac20_rna_substrate-rep-24 3725490 N/A 3725496 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3725501 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false 4B FCoV (feline coronavirus) nsp16 protein and SARS-CoV nsp10 protein. FCoV nsp16 could be stimulated to a similar level either by cognate nsp10 or noncognate nsp10s. pppac20_rna_substrate-rep-25 3725490 N/A 3725496 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3725503 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false 4B FCoV (feline coronavirus) nsp16 protein and MERS-CoV nsp10 protein. FCoV nsp16 could be stimulated to a similar level either by cognate nsp10 or noncognate nsp10s. pppac20_rna_substrate-rep-26 3725490 N/A 3725496 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3725505 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false 4B FCoV (feline coronavirus) nsp16 protein and IBV nsp10 protein. FCoV nsp16 could be stimulated to a similar level either by cognate nsp10 or noncognate nsp10s. pppac20_rna_substrate-rep-27 3725490 N/A 3725498 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725495 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4C MHV (mouse hepatitis virus) 16 protein and TGEV nsp10 protein. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-28 3725490 N/A 3725498 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725497 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4C MHV (mouse hepatitis virus) 16 protein and FCoV nsp10 protein. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-29 3725490 N/A 3725498 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725505 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4C MHV (mouse hepatitis virus) 16 protein and IBV nsp10 protein. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-30 3725490 N/A 3725500 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725499 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 3D, 4D SARS-CoV 16 proteins and MHV nsp10. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-31 3725490 N/A 3725500 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725503 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 3D, 4D SARS-CoV 16 proteins and MersCoV nsp10. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-32 3725490 N/A 3725500 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725495 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4D SARS-CoV 16 proteins and TGEV nsp10. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-33 3725490 N/A 3725500 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725497 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4D SARS-CoV 16 proteins and FCoV nsp10. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-34 3725490 N/A 3725500 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725505 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4D SARS-CoV 16 proteins and IBV nsp10. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-35 3725490 N/A 3725502 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725495 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4E MERS-CoV 16 protein and TGEV nsp10 protein. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-36 3725490 N/A 3725502 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725497 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4E MERS-CoV 16 protein and FCoV nsp10 protein. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-37 3725490 N/A 3725502 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725505 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 4E MERS-CoV 16 protein and IBV nsp10 protein. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-38 3725490 N/A 3725499 inhibitor inhibitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal Tyr ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res certain certain sequence position certain certain certain sequence position certain false C-terminal Phe amidated residue amidated residue certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3725497 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725496 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 5B, number 1 FCoV (feline coronavirus) nsp10+16 proteins. pppac20_rna_substrate-rep-39 3725490 N/A 3725499 inhibitor inhibitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal Tyr ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res certain certain sequence position certain certain certain sequence position certain false C-terminal Phe amidated residue amidated residue certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3725494 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725495 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P0C6Y5-PRO_0000037395:p.Lys93Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0C6Y5-PRO_0000037395:p.Phe96Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 5B number 2 TGEV (Porcine transmissible gastroenteritis coronavirus ) nsp10+16 proteins. pppac20_rna_substrate-rep-40 3725490 N/A 3725499 inhibitor inhibitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal Tyr ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res certain certain sequence position certain certain certain sequence position certain false C-terminal Phe amidated residue amidated residue certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3725498 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725499 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P0C6Y0-PRO_0000037363:p.Arg93Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0C6Y0-PRO_0000037363:p.Phe96Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 5B, number 3 MHV (mouse hepatitis virus) nsp10+16 proteins. pppac20_rna_substrate-rep-41 3725490 N/A 3725499 inhibitor inhibitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal Tyr ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res certain certain sequence position certain certain certain sequence position certain false C-terminal Phe amidated residue amidated residue certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3725500 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725501 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P0C6X7-PRO_0000037317:p.Tyr96Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0C6X7-PRO_0000037317:p.Lys93Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 5B, number 4 SARS-CoV nsp10+16 proteins. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-42 3725490 N/A 3725499 inhibitor inhibitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal Tyr ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res certain certain sequence position certain certain certain sequence position certain false C-terminal Phe amidated residue amidated residue certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3725502 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725503 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false K9N7C7-PRO_0000422448:p.Lys93Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false K9N7C7-PRO_0000422448:p.Phe96Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 5B, number 5 MERS-CoV nsp10+16 proteins. nsp16s of Betacoronavirus, including MHV, SARS-CoV , and MERS-CoV, could be fully activated and stimulated with the help of nsp10s of all selected coronaviruses from different genera. pppac20_rna_substrate-rep-43 3725490 N/A 3725499 inhibitor inhibitor neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal Tyr ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res certain certain sequence position certain certain certain sequence position certain false C-terminal Phe amidated residue amidated residue certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3725504 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725505 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P0C6Y1-PRO_0000037412:p.Lys98Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false P0C6Y1-PRO_0000037412:p.Phe101Ala mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 5B IBV (infectious bronchitis virus) nsp10+16 proteins. Identity of "IBV" confirmed from Ref.28 (PMID: 22022266) , Supp. figure 2 sequence. The nsp16 of IBV (Gammacoronavirus) was activated and stimulated y only by its cognate nsp10. Current Uniprot P0C6Y1 sequence numbering for IBV nsp10 differs from that given in figure 3A. pppac20_rna_substrate-rep-6 3725491 N/A 3725496 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false ATP 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 1C, number 2 FCoV (feline coronavirus) 16 protein. FCoV nsp16 alone generated a small fraction of products that comigrated with m7G*pppAm structure. showed a low but detectable MTase activity. pppac20_rna_substrate-rep-7 3725491 N/A 3725496 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false ATP 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3725497 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) methylation methylation reaction methylation false false false 1C, number 1 FCoV (feline coronavirus) nsp10+16 proteins. pppac20_rna_substrate-rep-8 3725491 N/A 3725500 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725501 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false ATP 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 1E SARS-CoV nsp10+16 proteins. paps-pppac20_rna_substrate-4 3725491 N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false ATP 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3725493 enzyme enzyme neutral component neutral component in vitro In vitro methylation methylation reaction methylation false false false 1H Positive control experiment; no information is given with respect to purification, expression or construct details for VP39. pppac20_rna_substrate-rep-9 3725491 N/A 3725494 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725495 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false ATP 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 1B TGEV (Porcine transmissible gastroenteritis coronavirus ) nsp10+16 proteins. pppac20_rna_substrate-rep-10 3725491 N/A 3725498 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725499 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false ATP 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 1D MHV (mouse hepatitis virus) nsp10+16 proteins. pppac20_rna_substrate-rep-11 3725491 N/A 3725502 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725503 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false atp 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 1F MERS-CoV nsp10+16 proteins. pppac20_rna_substrate-rep-12 3725491 N/A 3725504 enzyme enzyme neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725505 stimulator stimulator ancillary ancillary purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3725492 enzyme target enzyme target substrate substrate neutral component neutral component 5'_methyl_N7_Guanosine rna chemical modification RNA chemical modification RNA chemical modification post-transcriptional modification rna chemical modification certain certain sequence position certain certain certain sequence position certain false 2′-O-ribose methylated residue carbohydrate chemical modification carbohydrate chemical modification carbohydrate chemical modification certain certain sequence position certain certain certain sequence position certain false ATP 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain false in vitro In vitro methylation methylation reaction methylation false false false 1G IBV (infectious bronchitis virus) nsp10+16 proteins. Identity of "IBV" confirmed from Ref.28 (PMID: 22022266) , Supp. figure 2 sequence. rep-ddx1-2 3731885 N/A 3731891 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3731892 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B/C rep-ddx1-3 3731885 N/A 3731891 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 3731892 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1C rep-ddx1-1 3731886 N/A 3731891 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 3731892 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1A rep-ddx1-4 3731887 N/A 3731891 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 3731892 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1D self-activation was observed in all yeast colonies transformed with DDX1Δ2 (aas 536-631) rep-ddx1_chlae-1 3731889 N/A 3731894 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3731892 unspecified role unspecified role bait bait infection infection physical association physical association false false false 3 ddx1-rep-2 3731890 N/A 3731891 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Q92499:p.[Asp370_Glu371delinsAlaAla;Asp373Ala] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 3731892 unspecified role unspecified role neutral component neutral component infection infection colocalization colocalization false false false 8 aibv_rna_3'utr-pro_0000338322-1 3713900 N/A 3713904 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P0C6V3-PRO_0000338322:p.Gly98Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3713905 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false Fig. 4a (middle panel lanes 1 and 2) aibv_rna_antisense_3'utr-pro_0000338322-1 3713900 N/A 3713904 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P0C6V3-PRO_0000338322:p.Gly98Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3713906 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false Fig. 4a (bottom panel lanes 1 and 2) aibv_rna_3'utr-pro_0000338322-2 3713900 N/A 3713904 unspecified role unspecified role prey prey purified purified P0C6V3-PRO_0000338322:p.Lys51Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Lys90Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Lys10Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Tyr31Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Gly98Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Ile95Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Ile95Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Gly98Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3713905 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false Fig. 4a (middle panel lanes 3-8), Fig. 5b middle plane aibv_rna_3'utr-pro_0000338322-3 3713900 N/A 3713904 unspecified role unspecified role prey prey purified purified P0C6V3-PRO_0000338322:p.Lys10Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Ile95Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Gly98Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Ile95Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Tyr31Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Gly98Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false Only weak interaction was observed ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3713905 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false Fig. 4a (middle panel lanes 3-8), Fig. 5b middle panel Only weak interaction was observed for p.Gly98Asp mutant aibv_rna_antisense_3'utr-pro_0000338322-3 3713900 N/A 3713904 unspecified role unspecified role prey prey purified purified P0C6V3-PRO_0000338322:p.Gly98Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Ile95Asn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Gly98Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Ile95Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3713906 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false Fig. 4a (bottom panel lanes 3-8) aibv_rna_antisense_3'utr-pro_0000338322-4 3713900 N/A 3713904 unspecified role unspecified role prey prey purified purified P0C6V3-PRO_0000338322:p.Ile95Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Gly98Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Ile95Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Gly98Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3713906 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false Fig. 4a (bottom panel lanes 3-8) aibv_rna_3'utr-pro_0000338322-4 3713900 N/A 3713904 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P0C6V3-PRO_0000338322:p.Gly98Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3713905 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false Fig. 4a (middle panel lanes 1 and 2) aibv_rna_antisense_3'utr-pro_0000338322-5 3713900 N/A 3713904 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P0C6V3-PRO_0000338322:p.Gly98Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3713906 unspecified role unspecified role bait bait purified purified digoxigenin label dye labelled dye label dye labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false Fig. 4a (bottom panel lanes 1 and 2) pro_0000338322-1 3713901 N/A 3713904 unspecified role unspecified role neutral component neutral component purified purified P0C6V3-PRO_0000338322:p.Lys10Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Tyr31Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Lys51Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Lys90Ala mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Ile95Asn mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Gly98Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Ile95Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Gly98Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Figs. 3a, 5a pro_0000338322-2 3713902 N/A 3713904 unspecified role unspecified role neutral component neutral component purified purified P0C6V3-PRO_0000338322:p.Lys51Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Lys90Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Lys10Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Tyr31Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Ile95Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Gly98Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Figs. 3b, 5a pro_0000338322-pro_0000338322-1 3713903 N/A 3713904 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P0C6V3-PRO_0000338322:p.Ile95Asn mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false P0C6V3-PRO_0000338322:p.Gly98Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain false N/A 3713904 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 3c