DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ carter-2011-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Carter EL., Tronrud DE., Taber SR., Karplus PA., Hausinger RP. DIP original experiment: DIP-253898X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Iron-containing urease in a pathogenic bacterium. Carter EL., Tronrud DE., Taber SR., Karplus PA., Hausinger RP. Only protein-protein interactions imex curation carter-2011-2 helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Carter EL., Tronrud DE., Taber SR., Karplus PA., Hausinger RP. DIP original experiment: DIP-253901X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Iron-containing urease in a pathogenic bacterium. Carter EL., Tronrud DE., Taber SR., Karplus PA., Hausinger RP. Only protein-protein interactions imex curation carter-2011-3 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Carter EL., Tronrud DE., Taber SR., Karplus PA., Hausinger RP. DIP original experiment: DIP-253899X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Iron-containing urease in a pathogenic bacterium. Carter EL., Tronrud DE., Taber SR., Karplus PA., Hausinger RP. Only protein-protein interactions imex curation carter-2011-4 helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Carter EL., Tronrud DE., Taber SR., Karplus PA., Hausinger RP. DIP original experiment: DIP-253900X Proceedings of the National Academy of Sciences of the United States of America 2011 IMEx Iron-containing urease in a pathogenic bacterium. Carter EL., Tronrud DE., Taber SR., Karplus PA., Hausinger RP. Only protein-protein interactions imex curation d3uj81_helm1 Urease ureA2 HMU13020 protein protein helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae MKLTPKEQEKFLLYYAGEVARKRKEEGLKLNQPEAIAYISAHIMDEARRGKKTVAQLMEECVHFLKKDEVMPGVGNMVPDLGVEANFPDGTKLVTVNWPIEPDDFKAGEIKFASDKDIELNAGKEITELKVTNKGPKSLHVGSHFHFFEANRALEFDREKAYGKRLDIPSGNTLRIGAGETKTVHLIPIGGSKKIIGMNGLLNGIADDLHKQKALEKAKHHGFIK d3uj80_helm1 Urease subunit beta ureB Urea amidohydrolase subunit beta ureB2 HMU13010 protein protein helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae MKMKRQEYVNTYGPTTGDKVRLGDTDLWAEVEHDYTVYGEELKFGAGKTIREGMGQSNSPDENTLDLVITNALIIDYTGIYKADIGIKNGKIHGIGKAGNKDMQDGVTPHMVVGVGTEALAGEGMIITAGGIDSHTHFLSPQQFPTALANGVTTMFGGGTGPVDGTNATTITPGVWNLHRMLRAAEEYGMNVGLLGKGNSSSRAQLVEQVKAGAIGFKLHEDWGTTPSAIDHCLSVADEYDVQVCIHTDTVNEAGYVDDTLRAMNGRAIHAYHIEGAGGGHSPDVITMAGEVNILPSSTTPTIPYTINTVAEHLDMLMTCHHLDKRIREDLQFSQSRIRPGSIAAEDTLHDMGVIAMTSSDSQAMGRAGEVIPRTWQTADKNKKEFGRLTEEKGDNDNFRIKRYISKYTINPAITHGVSEYIGSVEEGKIADLVVWNPAFFGVKPKIIIKGGMVVFSEMGDSNASVPTPQPVYYREMFGHHGKAKFDTSITFVSKVAYENGIKEKLGLERKVLPVKNCRNVTKKDFKFNNTTAKITVNPETFEVFVNGKLCTSKPATEVALASRYTFF d3uge6_helm1 Urease subunit alpha ureA Urea amidohydrolase subunit alpha HMU03050 protein protein helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae MRLTPKELDKMMLHYAGELAKKRKERGVKLNYVEAVALISMEIMEHAREGKKTVADLMSLGRQVLKAEDVMDGVAALVHEVQIEVCFPDGTKLVTVHNPIENNGKLHPGEFILKDEDIVLNAGKEAIEVKVSNKGDRPIQVGSHFHFFETNKLLEFDREKAYGRRLDIASGTSVRFEPGESKTVRLIQFGGNQRIFGFNDLNNGQVNEDNKRKALAAAKAKGFIK d3uge7_helm1 Urease subunit beta ureB Urea amidohydrolase subunit beta HMU03060 protein protein helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae MIKISRKEYVSMYGPTTGDKVRLGDTELIAEIEKDYTVYGEEIKFGGGKTIRDGMSQSVSPDVNELDAVITNAMIIDYTGIYKADIGIKDGKIAGIGKAGNRDTQDGVGMDLVVGASTEAIAGEGLIVTAGGIDTHIHFISPTQIPTALYSGVTTMIGGGTGPAAGTFATTISPGEWNIKQMIRAAEEYTMNLGFFGKGNTSNVKALEDQIKAGALGFKVHEDWGSTPAVINHSLNIAEKYDVQVAIHTDTLNEGGAVEDTLAAIGGRTIHTFHTEGAGGGHAPDIIKAAGEANILPASTNPTIPFTKNTKDEHLDMLMVCHHLDKNIKEDVAFADSRIRPETIAAEDALHDMGIFSITSSDSQAMGRVGEVITRTWQVADKSKKEFGRLKEETGDNDNFRIKRYVSKYTINPAIAHGISDYIGSVEKGKIADLVLWDPGLFGIKPETIIKGGMIVASKMGDSNASIPTPEPVVYKDMFGAHGKAKYKCNVTFVSQASLDLGIKEELGLERTLLPVKNCRNIGKRDMKFNDVVAHIDVNPETYKVKVNGKEVTSKSADSVPLSQLYNLF urea2-ureb-1 3228858 N/A 3228862 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3228863 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false urea-ureb-12 3228859 N/A 3228864 unspecified role unspecified role unspecified role unspecified role helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae N/A 3228865 unspecified role unspecified role unspecified role unspecified role helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae direct interaction direct interaction false false false urea2-ureb-2 3228860 N/A 3228862 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3228863 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false urea2-ureb-3 3228861 N/A 3228862 unspecified role unspecified role unspecified role unspecified role helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae N/A 3228863 unspecified role unspecified role unspecified role unspecified role helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae direct interaction direct interaction false false false IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ marsh-2013-1 Protein complexes are under evolutionary selection to assemble via ordered pathways in vitro In vitro ms of complexes mass spectrometry studies of complexes ms of complexes predetermined predetermined participant predetermined Marsh J.A., Hernández H., Hall Z., Ahnert S.E., Perica T., Robinson C.V., Teichmann S.A. 2013-02-13: J. Marsh, European Bioinformatics Institute, Hinxton, UK jmarsh@ebi.ac.uk, sarah.teichmann@ebi.ac.uk Cell (0092-8674) 2013 Only protein-protein interactions imex curation accd_ecoli Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta accD dedB usg b2316 JW2313 protein protein ecoli Escherichia coli (strain K12) MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLEVCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKETGEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFSASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNIAEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAKLMNLPAPNPEAPREGVVVPPVPDQEPEA acca_ecoli Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha accA b0185 JW0180 protein protein ecoli Escherichia coli (strain K12) MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLREKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIVGGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITFIDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVNMLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAHRNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA ure1_kleae Urease subunit alpha ureC Urea amidohydrolase subunit alpha protein protein entae Enterobacter aerogenes Aerobacter aerogenes MSNISRQAYADMFGPTVGDKVRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGMGQGQMLAADCVDLVLTNALIVDHWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIGAATEVIAAEGKIVTAGGIDTHIHWICPQQAEEALVSGVTTMVGGGTGPAAGTHATTCTPGPWYISRMLQAADSLPVNIGLLGKGNVSQPDALREQVAAGVIGLKIHEDWGATPAAIDCALTVADEMDIQVALHSDTLNESGFVEDTLAAIGGRTIHTFHTEGAGGGHAPDIITACAHPNILPSSTNPTLPYTLNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRRETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVILRTWQVAHRMKVQRGALAEETGDNDNFRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVVWSPAFFGVKPATVIKGGMIAIAPMGDINASIPTPQPVHYRPMFGALGSARHHCRLTFLSQAAAANGVAERLNLRSAIAVVKGCRTVQKADMVHNSLQPNITVDAQTYEVRVDGELITSEPADVLPMAQRYFLF ure2_kleae Urease subunit beta ureB Urea amidohydrolase subunit beta protein protein entae Enterobacter aerogenes Aerobacter aerogenes MIPGEYHVKPGQIALNTGRATCRVVVENHGDRPIQVGSHYHFAEVNPALKFDRQQAAGYRLNIPAGTAVRFEPGQKREVELVAFAGHRAVFGFRGEVMGPLEVNDE cara_ecoli Carbamoyl-phosphate synthase small chain carA pyrA b0032 Carbamoyl-phosphate synthetase glutamine chain JW0030 protein protein ecoli Escherichia coli (strain K12) MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYLKRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAKEVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTIVPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLALASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFDGTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK carb_ecoli Carbamoyl-phosphate synthase large chain carB pyrA b0033 Carbamoyl-phosphate synthetase ammonia chain JW0031 protein protein ecoli Escherichia coli (strain K12) MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRVILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALELERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAADVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEMEVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIGVETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDELMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGADRIWYIADAFRAGLSVDGVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGVREAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLGGGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLEDVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAVERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQTAVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPAYTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKATGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRSTGEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELDATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIRRSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK trpa_salty Tryptophan synthase alpha chain trpA STM1727 protein protein salty Salmonella typhimurium MERYENLFAQLNDRREGAFVPFVTLGDPGIEQSLKIIDTLIDAGADALELGVPFSDPLADGPTIQNANLRAFAAGVTPAQCFEMLALIREKHPTIPIGLLMYANLVFNNGIDAFYARCEQVGVDSVLVADVPVEESAPFRQAALRHNIAPIFICPPNADDDLLRQVASYGRGYTYLLSRSGVTGAENRGALPLHHLIEKLKEYHAAPALQGFGISSPEQVSAAVRAGAAGAISGSAIVKIIEKNLASPKQMLAELRSFVSAMKAASRA trpb_salty Tryptophan synthase beta chain trpB STM1726 protein protein salty Salmonella typhimurium MTTLLNPYFGEFGGMYVPQILMPALNQLEEAFVSAQKDPEFQAQFADLLKNYAGRPTALTKCQNITAGTRTTLYLKREDLLHGGAHKTNQVLGQALLAKRMGKSEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVHSGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILDKEGRLPDAVIACVGGGSNAIGMFADFINDTSVGLIGVEPGGHGIETGEHGAPLKHGRVGIYFGMKAPMMQTADGQIEESYSISAGLDFPSVGPQHAYLNSIGRADYVSITDDEALEAFKTLCRHEGIIPALESSHALAHALKMMREQPEKEQLLVVNLSGRGDKDIFTVHDILKARGEI ure3_kleae Urease subunit gamma ureA Urea amidohydrolase subunit gamma protein protein entae Enterobacter aerogenes Aerobacter aerogenes MELTPREKDKLLLFTAALVAERRLARGLKLNYPESVALISAFIMEGARDGKSVASLMEEGRHVLTREQVMEGVPEMIPDIQVEATFPDGSKLVTVHNPII d3uj80_helm1 Urease subunit beta ureB Urea amidohydrolase subunit beta ureB2 HMU13010 protein protein helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae MKMKRQEYVNTYGPTTGDKVRLGDTDLWAEVEHDYTVYGEELKFGAGKTIREGMGQSNSPDENTLDLVITNALIIDYTGIYKADIGIKNGKIHGIGKAGNKDMQDGVTPHMVVGVGTEALAGEGMIITAGGIDSHTHFLSPQQFPTALANGVTTMFGGGTGPVDGTNATTITPGVWNLHRMLRAAEEYGMNVGLLGKGNSSSRAQLVEQVKAGAIGFKLHEDWGTTPSAIDHCLSVADEYDVQVCIHTDTVNEAGYVDDTLRAMNGRAIHAYHIEGAGGGHSPDVITMAGEVNILPSSTTPTIPYTINTVAEHLDMLMTCHHLDKRIREDLQFSQSRIRPGSIAAEDTLHDMGVIAMTSSDSQAMGRAGEVIPRTWQTADKNKKEFGRLTEEKGDNDNFRIKRYISKYTINPAITHGVSEYIGSVEEGKIADLVVWNPAFFGVKPKIIIKGGMVVFSEMGDSNASVPTPQPVYYREMFGHHGKAKFDTSITFVSKVAYENGIKEKLGLERKVLPVKNCRNVTKKDFKFNNTTAKITVNPETFEVFVNGKLCTSKPATEVALASRYTFF d3uj81_helm1 Urease ureA2 HMU13020 protein protein helm1 Helicobacter mustelae (strain ATCC 43772 / LMG 18044 / NCTC 12198 / 12198) Campylobacter mustelae MKLTPKEQEKFLLYYAGEVARKRKEEGLKLNQPEAIAYISAHIMDEARRGKKTVAQLMEECVHFLKKDEVMPGVGNMVPDLGVEANFPDGTKLVTVNWPIEPDDFKAGEIKFASDKDIELNAGKEITELKVTNKGPKSLHVGSHFHFFEANRALEFDREKAYGKRLDIPSGNTLRIGAGETKTVHLIPIGGSKKIIGMNGLLNGIADDLHKQKALEKAKHHGFIK cara-carb-7 1365431 n/a 1365432 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 4.0 n/a 1365433 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 4.0 direct interaction direct interaction false false false Supplementary Table 2 cara-carb-8 1365431 n/a 1365433 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 3.0 n/a 1365432 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 3.0 direct interaction direct interaction false false false Supplementary Table 2 cara-carb-9 1365431 n/a 1365433 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 n/a 1365432 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Supplementary Table 2 cara-carb-10 1365431 n/a 1365432 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 1.0 n/a 1365433 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 1.0 direct interaction direct interaction false false false Supplementary Table 2 trpa-trpb-22 1365431 n/a 1365434 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 n/a 1365435 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Supplementary Table 2 trpa-trpb-23 1365431 n/a 1365434 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 1.0 n/a 1365435 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Supplementary Table 2 trpb-trpb 1365431 n/a 1365435 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Supplementary Table 2 acca-accd-5 1365431 n/a 1365436 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 n/a 1365437 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false acca-acca 1365431 n/a 1365437 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli Stoichiometry: 1.0 n/a 1365436 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 n/a 1365437 unspecified role unspecified role neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli Stoichiometry: 1.0 physical association physical association false false false Supplementary Table 2 acca-accd 1365431 n/a 1365436 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 1.0 n/a 1365437 unspecified role unspecified role neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli Stoichiometry: 1.0 direct interaction direct interaction false false false Supplementary Table 2 acca-accd-6 1365431 n/a 1365436 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 1.0 n/a 1365437 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli Stoichiometry: 1.0 direct interaction direct interaction false false false Supp. Table 2 acca-accd-7 1365431 n/a 1365436 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli Stoichiometry: 2.0 n/a 1365437 unspecified role unspecified role neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Supplementary Table 2 urea-ureb-7 1365431 n/a 1365438 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 3.0 n/a 1365439 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 3.0 n/a 1365440 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 3.0 physical association physical association false false false Supplementary Table 2 urea-ureb-8 1365431 n/a 1365439 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 2.0 n/a 1365440 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 3.0 n/a 1365438 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 3.0 physical association physical association false false false Supplementary Table 2 urea-ureb-9 1365431 n/a 1365438 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 3.0 n/a 1365440 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 3.0 n/a 1365439 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 1.0 physical association physical association false false false Supplementary Table 2 urea-ureb-10 1365431 n/a 1365440 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 3.0 n/a 1365438 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 3.0 direct interaction direct interaction false false false Supplementary Table 2 urea-ureb-11 1365431 n/a 1365439 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 1.0 n/a 1365438 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 2.0 n/a 1365440 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 3.0 physical association physical association false false false Supplementary Table 2 urea-1 1365431 n/a 1365438 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 2.0 physical association physical association false false false Supplementary Table 2 urec 1365431 n/a 1365440 unspecified role unspecified role neutral component neutral component entae Enterobacter aerogenes Aerobacter aerogenes Stoichiometry: 3.0 direct interaction direct interaction false false false Supplementary Table 2 urea2-ureb2 1365431 n/a 1365441 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 12.0 n/a 1365442 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 12.0 direct interaction direct interaction false false false Supp. Table 2 urea2-ureb2-1 1365431 n/a 1365442 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 9.0 n/a 1365441 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 9.0 direct interaction direct interaction false false false Supp. Table 2 urea2-ureb2-2 1365431 n/a 1365442 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 6.0 n/a 1365441 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 6.0 direct interaction direct interaction false false false Supp. Table 2 urea2-ureb2-3 1365431 n/a 1365441 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 3.0 n/a 1365442 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 3.0 direct interaction direct interaction false false false Supp. Table 2 urea2-ureb2-4 1365431 n/a 1365442 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 n/a 1365441 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 3.0 direct interaction direct interaction false false false Supp. Table 2 urea2-ureb2-5 1365431 n/a 1365442 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 n/a 1365441 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Table 2 urea2-ureb2-6 1365431 n/a 1365442 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 3.0 n/a 1365441 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 1.0 direct interaction direct interaction false false false Supp. Table 2 urea2-ureb2-7 1365431 n/a 1365442 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 n/a 1365441 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 1.0 direct interaction direct interaction false false false Supp. Table 2 urea2-ureb2-8 1365431 n/a 1365441 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Table 2 urea2-ureb2-9 1365431 n/a 1365442 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 2.0 direct interaction direct interaction false false false Supp. Table 2 urea2-10 1365431 n/a 1365442 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 3.0 direct interaction direct interaction false false false Supp. Table 2 urea2-ureb2-10 1365431 n/a 1365441 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 1.0 n/a 1365442 unspecified role unspecified role neutral component neutral component ecolx Escherichia coli Stoichiometry: 1.0 direct interaction direct interaction false false false Supp. Table 2