DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wang-2015-47 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. DIP original experiment: DIP-267134X Nature 2015 IMEx Allosteric receptor activation by the plant peptide hormone phytosulfokine. Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. Only protein-protein interactions imex curation wang-2015-48 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. DIP original experiment: DIP-267127X Nature 2015 IMEx Allosteric receptor activation by the plant peptide hormone phytosulfokine. Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. Only protein-protein interactions imex curation wang-2015-54 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. DIP original experiment: DIP-267140X Nature 2015 IMEx Allosteric receptor activation by the plant peptide hormone phytosulfokine. Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. Only protein-protein interactions imex curation wang-2015-55 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. DIP original experiment: DIP-267137X Nature 2015 IMEx Allosteric receptor activation by the plant peptide hormone phytosulfokine. Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. Only protein-protein interactions imex curation wang-2015-59 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. DIP original experiment: DIP-267136X Nature 2015 IMEx Allosteric receptor activation by the plant peptide hormone phytosulfokine. Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. Only protein-protein interactions imex curation wang-2015-60 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. DIP original experiment: DIP-267128X Nature 2015 IMEx Allosteric receptor activation by the plant peptide hormone phytosulfokine. Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. Only protein-protein interactions imex curation wang-2015-63 in vitro In vitro mst microscale thermophoresis mst predetermined predetermined participant predetermined Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. DIP original experiment: DIP-267129X Nature 2015 IMEx Allosteric receptor activation by the plant peptide hormone phytosulfokine. Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. Only protein-protein interactions imex curation wang-2015-64 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. DIP original experiment: DIP-267135X Nature 2015 IMEx Allosteric receptor activation by the plant peptide hormone phytosulfokine. Wang J., Li H., Han Z., Zhang H., Wang T., Lin G., Chang J., Yang W., Chai J. Only protein-protein interactions imex curation serk2_arath Somatic embryogenesis receptor kinase 2 SERK2 Somatic embryogenesis receptor-like kinase 2 F23M19.11 At1g34210 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGRKKFEAFGFVCLISLLLLFNSLWLASSNMEGDALHSLRANLVDPNNVLQSWDPTLVNPCTWFHVTCNNENSVIRVDLGNADLSGQLVPQLGQLKNLQYLELYSNNITGPVPSDLGNLTNLVSLDLYLNSFTGPIPDSLGKLFKLRFLRLNNNSLTGPIPMSLTNIMTLQVLDLSNNRLSGSVPDNGSFSLFTPISFANNLDLCGPVTSRPCPGSPPFSPPPPFIPPPIVPTPGGYSATGAIAGGVAAGAALLFAAPALAFAWWRRRKPQEFFFDVPAEEDPEVHLGQLKRFSLRELQVATDSFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPPSQLPLAWSIRQQIALGSARGLSYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLARLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGIMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEMLVDPDLQSNYTEAEVEQLIQVALLCTQSSPMERPKMSEVVRMLEGDGLAEKWDEWQKVEVLRQEVELSSHPTSDWILDSTDNLHAMELSGPR pskr1_dauca Phytosulfokine receptor 1 PSKR Phytosulfokine LRR receptor kinase 1 protein protein dauca Daucus carota Wild carrot MGVLRVYVILILVGFCVQIVVVNSQNLTCNSNDLKALEGFMRGLESSIDGWKWNESSSFSSNCCDWVGISCKSSVSLGLDDVNESGRVVELELGRRKLSGKLSESVAKLDQLKVLNLTHNSLSGSIAASLLNLSNLEVLDLSSNDFSGLFPSLINLPSLRVLNVYENSFHGLIPASLCNNLPRIREIDLAMNYFDGSIPVGIGNCSSVEYLGLASNNLSGSIPQELFQLSNLSVLALQNNRLSGALSSKLGKLSNLGRLDISSNKFSGKIPDVFLELNKLWYFSAQSNLFNGEMPRSLSNSRSISLLSLRNNTLSGQIYLNCSAMTNLTSLDLASNSFSGSIPSNLPNCLRLKTINFAKIKFIAQIPESFKNFQSLTSLSFSNSSIQNISSALEILQHCQNLKTLVLTLNFQKEELPSVPSLQFKNLKVLIIASCQLRGTVPQWLSNSPSLQLLDLSWNQLSGTIPPWLGSLNSLFYLDLSNNTFIGEIPHSLTSLQSLVSKENAVEEPSPDFPFFKKKNTNAGGLQYNQPSSFPPMIDLSYNSLNGSIWPEFGDLRQLHVLNLKNNNLSGNIPANLSGMTSLEVLDLSHNNLSGNIPPSLVKLSFLSTFSVAYNKLSGPIPTGVQFQTFPNSSFEGNQGLCGEHASPCHITDQSPHGSAVKSKKNIRKIVAVAVGTGLGTVFLLTVTLLIILRTTSRGEVDPEKKADADEIELGSRSVVLFHNKDSNNELSLDDILKSTSSFNQANIIGCGGFGLVYKATLPDGTKVAIKRLSGDTGQMDREFQAEVETLSRAQHPNLVHLLGYCNYKNDKLLIYSYMDNGSLDYWLHEKVDGPPSLDWKTRLRIARGAAEGLAYLHQSCEPHILHRDIKSSNILLSDTFVAHLADFGLARLILPYDTHVTTDLVGTLGYIPPEYGQASVATYKGDVYSFGVVLLELLTGRRPMDVCKPRGSRDLISWVLQMKTEKRESEIFDPFIYDKDHAEEMLLVLEIACRCLGENPKTRPTTQQLVSWLENIDVSS psk Phytosulfokine peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) YIYTQ serk1_arath Somatic embryogenesis receptor kinase 1 SERK1 Somatic embryogenesis receptor-like kinase 1 F14O23.21 F14O23_24 At1g71830 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MESSYVVFILLSLILLPNHSLWLASANLEGDALHTLRVTLVDPNNVLQSWDPTLVNPCTWFHVTCNNENSVIRVDLGNAELSGHLVPELGVLKNLQYLELYSNNITGPIPSNLGNLTNLVSLDLYLNSFSGPIPESLGKLSKLRFLRLNNNSLTGSIPMSLTNITTLQVLDLSNNRLSGSVPDNGSFSLFTPISFANNLDLCGPVTSHPCPGSPPFSPPPPFIQPPPVSTPSGYGITGAIAGGVAAGAALLFAAPAIAFAWWRRRKPLDIFFDVPAEEDPEVHLGQLKRFSLRELQVASDGFSNKNILGRGGFGKVYKGRLADGTLVAVKRLKEERTPGGELQFQTEVEMISMAVHRNLLRLRGFCMTPTERLLVYPYMANGSVASCLRERPPSQPPLDWPTRKRIALGSARGLSYLHDHCDPKIIHRDVKAANILLDEEFEAVVGDFGLAKLMDYKDTHVTTAVRGTIGHIAPEYLSTGKSSEKTDVFGYGIMLLELITGQRAFDLARLANDDDVMLLDWVKGLLKEKKLEMLVDPDLQTNYEERELEQVIQVALLCTQGSPMERPKMSEVVRMLEGDGLAEKWDEWQKVEILREEIDLSPNPNSDWILDSTYNLHAVELSGPR pskr-serk2-1 3282939 N/A 3282961 unspecified role unspecified role unspecified role unspecified role Region 24-659 Region 24-659 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3282962 unspecified role unspecified role unspecified role unspecified role Region 1-216 Region 1-216 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9XIC7:p.Thr62Tyr T62Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false pskr-serk2-2 3282940 N/A 3282961 unspecified role unspecified role bait bait Region 24-659 Region 24-659 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q8LPB4:p.Phe606Asp F606D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8LPB4:p.Ser608Tyr S608Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8LPB4:p.Thr629Tyr T629Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3282962 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 1-216 Region 1-216 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false psk-pskr-1 3282946 N/A 3282962 unspecified role unspecified role unspecified role unspecified role Region 1-216 Region 1-216 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3282966 unspecified role unspecified role unspecified role unspecified role sY1/3 sY1/3 osulftyr O4'-sulfo-L-tyrosine (S)-2-amino-3-(4-sulfooxyphenyl)propanoic acid MOD_RES Sulfotyrosine O4-sulfotyrosine tyrosine sulfate tyrosine-O-sulfonic acid tyrosine-O-sulphonic acid 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-sulfate O4'-sulfo-L-tyrosine O-Sulfonation OSulfTyr Sulfo Sulphation (of O of Tyrosine) Tyrosinyl Sulphate sulfationy certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3282961 unspecified role unspecified role unspecified role unspecified role Region 24-659 Region 24-659 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false psk-pskr-2 3282947 N/A 3282966 unspecified role unspecified role unspecified role unspecified role sY1/3 sY1/3 osulftyr O4'-sulfo-L-tyrosine (S)-2-amino-3-(4-sulfooxyphenyl)propanoic acid MOD_RES Sulfotyrosine O4-sulfotyrosine tyrosine sulfate tyrosine-O-sulfonic acid tyrosine-O-sulphonic acid 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-sulfate O4'-sulfo-L-tyrosine O-Sulfonation OSulfTyr Sulfo Sulphation (of O of Tyrosine) Tyrosinyl Sulphate sulfationy certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3282961 unspecified role unspecified role unspecified role unspecified role Region 24-659 Region 24-659 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false pskr-serk2-3 3282951 N/A 3282961 unspecified role unspecified role unspecified role unspecified role Region 24-659 Region 24-659 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8LPB4:p.Phe606Asp F606D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8LPB4:p.Ser608Tyr S608Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8LPB4:p.Thr629Tyr T629Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3282962 unspecified role unspecified role unspecified role unspecified role Region 1-216 Region 1-216 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false pskr-serk2-4 3282952 N/A 3282961 unspecified role unspecified role bait bait Region 24-659 Region 24-659 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress N/A 3282962 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 1-216 Region 1-216 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9XIC7:p.Thr62Tyr T62Y mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress physical association physical association false false false psk-pskr-3 3282955 N/A 3282966 unspecified role unspecified role unspecified role unspecified role sY1/3 sY1/3 osulftyr O4'-sulfo-L-tyrosine (S)-2-amino-3-(4-sulfooxyphenyl)propanoic acid MOD_RES Sulfotyrosine O4-sulfotyrosine tyrosine sulfate tyrosine-O-sulfonic acid tyrosine-O-sulphonic acid 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-sulfate O4'-sulfo-L-tyrosine O-Sulfonation OSulfTyr Sulfo Sulphation (of O of Tyrosine) Tyrosinyl Sulphate sulfationy certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3282961 unspecified role unspecified role unspecified role unspecified role Region 24-659 Region 24-659 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8LPB4:p.Phe516Ala F516A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8LPB4:p.Arg310Ala R310A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8LPB4:p.Trp458Ala W458A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8LPB4:p.Thr408Leu T408L mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8LPB4:p.Asp455Ala D455A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false pskr-serk1-1 3282956 N/A 3282965 unspecified role unspecified role unspecified role unspecified role Region 1-213 Region 1-213 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3282961 unspecified role unspecified role unspecified role unspecified role Region 24-659 Region 24-659 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false