IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ haag-2002-1 Rapid coevolution of the nematode sex-determining genes fem-3 and tra-2. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Haag ES., Wang S., Kimble J. Fig 4A, 4B ehaag@wam.umd.edu Curr. Biol. (0960-9822) 2002 fem3_caeel Sex-determination protein fem-3 Feminization of XX and XO animals protein 3 Ce-FEM-3 C01F6.4 fem-3 protein protein caeel Caenorhabditis elegans MEVDPGSDDVEADRETRAQKLKLKRNVKFRAQMRRFDEYCGVTNLTVDDLNWPLISGIPLQRQRLTGATYYDDSLLDQNPWDEFSIDRFLEITSIQLITAGAGYERNDEITRFVFQRTMKTIVTYCNFMYDLARRNGKVQITRFELQDLIHRDEFRFYMYFRQFLPNPDPNCTAFSNHYTSLLHTLYFNIPGMPQFWNNSQMYNYAATRGQRLVQNIAAFYPPEYFWNEDESKYHTTFVVPRGTEFSKFYARRFHEALGMPPLENEIITVLDWLAKLCILEIVYHTTIWCDITGFGGLPRIEHYRLAMENVEDIIFDLAIDDFSISRLQLQISPFEISRYSPLDVSGYYETIKRKKDIEEYQNRFYEVHYSDDVRIMNVYATDCSRKR tra2_caeel Sex-determining transformer protein 2 tra-2 C15F1.3 Ce-Tra-2 protein protein caeel Caenorhabditis elegans MKLKYNKLLVSVVIVTFVTFGLLLAECFGKSIDYQEKSIFPSFVSQGFFETRTNNEEYIIEKIAQTQENGVDMRSTLHFTQHGYLLNNISNLKIKFRQKTYTLNDVCFKPHITIFQQSSSSDQNEYPHYIQRLLLEMQRLSPCLIVTPLNCFYDIYRIHGEISNWNKNTDFLNRRLRNSYIEAIGENDERPYVKSNYGPSLIKSWADHMFDLPSKSFTNSTKDALFQKIKLWLLSIEPRQKTCAASIHSCDTPLDSEHYFNICTDMQSVDNFAEKKTKFKLEDVDEEFAMNLDCVDDQEQFIEWMQELEIRKMYSHVTEKPDYPNVVNQTCDKIFHDLNSTGIEFFDGSRSFSSTKSQFDTMQTEIVLLTPEMLLSAMQHSDFVNGFESIWTIEKAEELIHEFRLALKEETEKFKENRMSKMIRVTSRVLDNTVTTKLQSFSEKQTIHFVVNVHSLIVILFTIFVWSGAPLRSAFMFFVRDALTCLLFCFVCSTDGVIVLDTELIKYIIVLTLANLYFTTRSSFCTERLSRCIQREKRFPINSNFASLITVDTMTDSRQIQYFLSTVTKYQAAQDSYSNELFERFPKNWGCTSILIFPIVFVYWYFIDSNFDKICVSVLPSFCLAAGEELFAKNMFWKEREAMQAKQRLENEEQAESITGSSLEKLFAGNKPVSNTDKANIVKKSSIIRNQKPCLQDLSPGTYDVSNFMKYPHQASRIFREKIIGLYLRILKLRTLGVILCIPAILLIVISIGLLFIPVKRETLHTDSKQDDIFIEFEIFNFSTNWKIVNQNLKQFSEDIESIGTLYTISNWQKSFERFEQETNKNASAEWNILFKWINDEPINSAVTLFSEKSSGNQTIANPFKFRLRYGFDAKNETTVIEIVQKIDELLSKCSKNLSPKAVGVLYEHYHRIAVVWNLFAFNQLTTAGIFIILLSIITFIFAITPTIKATFLFSLLVVGTQIEVAALVHLFSLDHHQIYTNLALFAGFLAAWDPFCALLRYRRRILYKSETRRTPELASKRRVLLPIVATADIAQFFVLLITAFSILAIICSIVPELNIFFVPTVILIVIQIVAVFNSIIVSIATKQMFESEVRHYLHRDLRGSTTAVRVYNLVQKQRLASSLDEPQVELDEFSIKRSSPPCRYYAPPPKYSCKKRSRSSDEDEDSDPNQPGPSNRRSPKTGNKRVRGNGDNTELYIPNRYELIVSGKSVGGNTSAAWNGPGSTLEQNMNALEECFELGVDEYDFDEHDGDEGCELVQDMLDRERNLMNKRSTAQRRESRNIEKMKKSQENLDKEKSEEKISESKKNQDDSIESPNLPGTPANLPVDEPLPPVGRLYIVEHVLPEEYRRDPLTEPPSMEDCIRAHSDPNLPPHPRADQYPASFTRPMVEYCEDIYWTHRTGQLPPGLQVPRRPYDYYHITERTPPPEDLNWVPPAESPPIPIPQQAFDLLEERRRNHREQQDEAREGDLSDPEV fem3_caebr Sex-determination protein fem-3 Cb-FEM-3 fem-3 CBG21774 protein protein caebr Caenorhabditis briggsae MVPDDVEPMEVDDDGALIVDLNETVEEDEETKKEKKRRKRFREKLKRFDHYSQFSGISIAQIDWPLIQGRSLQRSPLTGQSFNADENIFRIDEWPRETFLQITSTLTFCAGAALLSNEKITLFVFQRTMKTLVAYCNFMYHRAITHNRRQINRIDVHELISRNPLRFHMFLQKFLPHPDINRTHFNNEFLYYFHNLYFQDETCRLLYHDVARYSPIINQQGTRMSLQHQIYYPDVMRNPAFDALWFTSFINPSGYSFSRFHAYRFHEALGMPPLESELIIVLDWLAKLIICDIGYKVLAWRDARGFQGLPDLLSFQMAMLEEGDPLFDLDIDYTAPPTRLFSEPTRFQTYPKFQPRRRIDFPSRFDGFYKKRRLERGLEEIQESFIMNHFPTKPLRTVYVYTHPEERRR tra2_caebr Sex-determining transformer protein 2 tra-2 CBG11194 protein protein caebr Caenorhabditis briggsae MKLAFNKLLVASVVFTVLSFGLLLASLFTTTATTPSEWTILLPEFRFPVNKKQTTEQFLVEKIVHEHEEGEDVRSALYLTHHGYFMNAIANMKVTYRQKSYTVNDVCFKPHSAIFENVPAPENIDKLPAYFQRLLLEMQRLSPCLIVTPLNCFYDSYHIHSEISNWNASTDYLNRRLRNSYLEAIEEKDSRPYVKSTYGPELIKEWARHMFAIPSKPLSNFSKSDLYSRVKTWLSSIAARKKICADPMRSCDETLDAENYFNVCTVMQQINDYDERRKQRLKFQLEYGDEEFTTRLDCVEDREKFIEWMQERNLRDMLKLFASSVEIPDHKEIPNQVCDGIYHDLDTSSGLELFRGARSFSNNTSAYDTINVELGFMTPENLLTTMRHSDFVNGFESIWTIERARELLNEFRLALKVEVTKFSESRSSRRVKVTTRIVNQIEEEGSDEEMEYHMIYFILGACALMVALFAAFAFSEAFLTSLSMFLLRGFITGLLFIFLCKSGGLILIDSNFLCYITMHLAFNLVMTARVTFICYRIGGCVQSEKDFVKSNFSSLGSVPVDSLKEDSCKRHVQYVLAKYTKFQVAQDAYSEEPFEKLPKYWFLIAIVLVPVIGVYWFFIDSDVQKICIVLLPAFLIAAFEEMRVKNQLLRERRIKKAIQRLQKEENTRIMSRGEIDNLLSGNAELSGEKSHYESKQGVLHHGSAGGLFELSRSTYDVSLIMAYPNQMIRNLRLCALGAYFRLFKMKYCAVVVSSVAALLILLSIGLLFIPVQRSSVPKELQQDELSIDFAIPNVSSSSWESINEYLEEFNSEIDSITNLQTITNGRRVLINSTSKINNFLKWVDDEPISWYLTAPLTRPYRKTHLPNPFRFQFRYGFDSIQKSTIIDVVERIDTLLTKYTETLSFPKAIGFLYEHYHQKAVVWNSFAYHEIFAAAVLAGFFSIIVVFFSIGPVVLPTLAFAFFVVGNRLEIAAIVSLFSLEYHQCYTNVAVFVGFLAAWTPFCDLARFRGRLLYKDQTRRTPELATQRRIRVPHVAAVDTVQIFAIFLTATILLIVITAIIPQFRAFFIPTVILLITLLLAVFNSLAVSLAAYQMFEHEVRHCYHDQLQSLTTTGKVCDMTRKKLLPREEDLSIPMEEFSIRPTENTKHYAPRPIDNSDPPEQAADEEVVNQDPSMEAARRQYVEFTHRTTGMPIELINQFVDNFPVFNVPANFLPNYFALGGAPLDANNGVLLRQPGIAPPPRPNREEDEEERFGLGGGEDDDSYPSSGDDIGDPAKEQQEVTDDVATRYKEEEVRKKVQPAVPNYDDPNVPGPSNPVPRQVEQVSREAPEDSPNREPRILVYQRPPRLHEIPQISHGRNPLHDPPSMEEYVQKYDDPNQPPSRRADQYPPSFTPAMVGYCEDVYWKYNERNLPDNVPMPPRPRDWDQRRLVELPPPEDFDEVPPPGRSAIPIPPGAIRLRERRREQHLREQEARRNRPESPDDTPGL fem3_caere Sex-determination protein fem-3 Cr-FEM-3 fem-3 protein protein caere Caenorhabditis remanei Caenorhabditis vulgaris MTCDDFRAEDEQPIEVDERRRRLKRKANDDDDDDETVRERVDDAESSMEVFEAEYPPILDPLQDQREAKYFRERMQRFDLYSRTTGLSVDDIDWPLIRGRSLQKGRVAGISFVYDDTRYPINRFSDTWLLCVTKQKLFSFGAGCVEDLNITSFVLRRTMKVLSTYCNWLFEAAKRNNRRHITHKEIQELINRDGFRFHQYLQKFLIGRGMEYTEYNNRFFKYLHEEYNKNPGGLETIYSNQDFIAKETAQANYIYDTVRAKYGGFEELPLFRHALKISFTQPGEHYFSRFYAKRFHEALGCPPLDSEIIMILDWFGVLIMNQIAYKTIRWHEEEYNDGSFPVLDSYHKALADESKCPKACLISISLFPDDPLFDLDIDYGTNPPPVNTAYQKVRRTPRDPTPFYRLMEFEDYKSCLVKMHFNFSELTGEWLRKICARGR tra2_caere Sex-determining transformer protein 2 Cr-tra-2 tra-2 protein protein caere Caenorhabditis remanei Caenorhabditis vulgaris MSLRSNKLLVAAVIFTVVTFGLLLTSSIFNNKTTTSLTYGGILPKFGQRIIEKKSNEEYIIEKIEHTQKDGEDVRSTRYLTHHSYLLRNLAKMEVKHHGKDFSINDICYKPHNAIFETTFAPENIDKLPNYLQRLILEAQRLSPCLIVTPLNCYHDSYRIHSEMSKWNTSNVTNFLNRKLRNSYIDAIGESEERPYIKSTYGPDLIKEWAHLMFKLPSKQTSSFSKKDLSSKIELWLSSIESKTNLTELGRPSEVDNYFDICTSMQQVHDFDERKRKFGLYDDDDEFLIGLDCVENKTKFIEWIQERELRGVSKPFNPNQQCDGIFKNSEGLEFFYGTRSFGNNTAPFDKMKTEIGLMTPEQILTTMLHSDFVNGFESIWTIERAQELLDDFRLAIRQEVKRFNENRSSLKIGIDTRVVQREESNETELEISSNLDSVVYFILFIRCVLLIFFAFFAWSVNPLRSAVMFLVRDALTSLLFSILCKSDGQIELNSELLGYIILLTIVNTYLTTRVSWYKDRNETCIQRAKDFPSRSNFSLLFSIDSLRENCDSRQLQYALAKLSKYLTALDTYSTETFMQLPNYWPFISILFVPITGCYWYFVDINLPKISVVLLPSFIVATIEQRQVEKSLLKERKAKKEFQKVQKKKMEKFLSDGAVDRLLSGNSESVEDKKLYKSKDCVIHKESAGRLYELSRSSYDVSKIMAYPNQRVRDFRFDALGCYFWLMKLKSAGILLYGSAVLFVLLSVAVMLIPIQRTSLQKDMNNEVYFGFSINNMSSDWANINKNLHAFNSEIDSIQSLQTISNWKKGFDRFEGRFYKNSSRISDANDYVEWMNQEPINWSVMAPLTRISPKFGIPSPFKFRFRYQIEVNNNESEVIDTVQRIDTLLTKYKGTLSSPIVDGVLYEYYHGNAAVWNSFVFNELLASGILSAFFALIVVIFSITPSISSVLIFSFFVIGSRLEIAAVISLFSLDNQQLYTDSAVLVGFLVAWAPFYELSLFRRRLLYKLKTRCTPELSSGKRIRPPFTKAVDTAQVFAIVLAASLIIAVVAGVVPEFQKFFWPTVILIVVQLVAFGNSIAVLVATNQMFEREVRNFLDNEFELGNGTTAGQVCHMAQKLIPPKYDIPIPMNDFHIRPTNMSKFYAPPPAKKRAKQTNNETDPEKKEDEPGTSNANNVSQEEAAHRLAILPWHFVLGGIPVDLTTRSDQIINGPFIGISSDAMRTHEINSELEDQDDYSSESSVEDVESDPAPEEEIKYHEENMLHMIEKVQKDAAEKEAKEKVHQVESAQRRAPNFDDPNVAGPSHRYQRNEERISTDIVPADPPREIPANPVPPPTHVLVQRAPRPHEMPPVIDRTIPRDPRTEPPNLQECIQQNDDPSLPPHPRRHQYPDHYGRAMISYCEDVYWTYNDGRLPPNVAMPPRPFDWHYRRVAPPEDFNYVPPPGQPSIPIPAEAMALREERARAHREQEQRDNSQSPSPSPEPGL fem3-tra2-3 Y2H interaction detected between tra2 and fem3 in C.elegans. 143922 N/A 143923 unspecified role unspecified role prey prey N/A 143924 unspecified role unspecified role bait bait c-terminus region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Residue numbering taken from Lum et al 2000 who donated construct fem3-tra2-4 Y2H interaction detected between tra2 and fem3 in C.briggsae. 143922 N/A 143925 unspecified role unspecified role prey prey N/A 143926 unspecified role unspecified role bait bait c-terminus region binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false fem3-tra2-5 Y2H interaction detected between tra2 and fem3 in C.remanei 143922 N/A 143927 unspecified role unspecified role prey prey N/A 143928 unspecified role unspecified role bait bait c-terminus region binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false