DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ pauwels-2006-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pauwels K., Lustig A., Wyns L., Tommassen J., Savvides SN., Van Gelder P. DIP original experiment: DIP-66219X Nature structural & molecular biology 2006 IMEx Structure of a membrane-based steric chaperone in complex with its lipase substrate. Pauwels K., Lustig A., Wyns L., Tommassen J., Savvides SN., Van Gelder P. Only protein-protein interactions imex curation pauwels-2006-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pauwels K., Lustig A., Wyns L., Tommassen J., Savvides SN., Van Gelder P. DIP original experiment: DIP-66220X Nature structural & molecular biology 2006 IMEx Structure of a membrane-based steric chaperone in complex with its lipase substrate. Pauwels K., Lustig A., Wyns L., Tommassen J., Savvides SN., Van Gelder P. Only protein-protein interactions imex curation pauwels-2006-3 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Pauwels K., Lustig A., Wyns L., Tommassen J., Savvides SN., Van Gelder P. DIP original experiment: DIP-66221X Nature structural & molecular biology 2006 IMEx Structure of a membrane-based steric chaperone in complex with its lipase substrate. Pauwels K., Lustig A., Wyns L., Tommassen J., Savvides SN., Van Gelder P. Only protein-protein interactions imex curation lip_burpl Triacylglycerol lipase Extracellular lipase Triacylglycerol ester hydrolase lip lipA protein protein burpl Burkholderia plantarii MVRSMRSRVAARAVAWALAVMPLAGAAGLTMAASPAAVAADTYAATRYPVILVHGLAGTDKFANVVDYWYGIQSDLQSHGAKVYVANLSGFQSDDGPNGRGEQLLAYVKQVLAATGATKVNLIGHSQGGLTSRYVAAVAPQLVASVTTIGTPHRGSEFADFVQDVLKTDPTGLSSTVIAAFVNVFGTLVSSSHNTDQDALAALRTLTTAQTATYNRNFPSAGLGAPGSCQTGAATETVGGSQHLLYSWGGTAIQPTSTVLGVTGATDTSTGTLDVANVTDPSTLALLATGAVMINRASGQNDGLVSRCSSLFGQVISTSYHWNHLDEINQLLGVRGANAEDPVAVIRTHVNRLKLQGV lifo_burpl Lipase-specific foldase Lipase helper protein Lipase activator protein Lipase modulator lifO lipB Lipase chaperone protein protein burpl Burkholderia plantarii MAQADRPARGGLAARPMRGASFALAGLVACAACAAVVLWLRPAAPSPAPAGAVAGGPAAGVPAAASGAAEAAMPLPAALPGALAGSHAPRLPLAAGGRLARTRAVREFFDYCLTAQGELTPAALDALVRREIAAQLDGSPAQAEALGVWRRYRAYFDALAQLPGDGAVLGDKLDPAAMQLALDQRAALADRTLGEWAEPFFGDEQRRQRHDLERIRIANDTTLSPEQKAARLAALDAQLTPDERAQQAALHAQQDAVTKIADLQKAGATPDQMRAQIAQTLGPEAAARAAQMQQDDEAWQTRYQAYAAERDRIAAQGLAPQDRDARIAQLRQQTFTAPGEAIRAASLDRGAGG lifo-lipa-1 3142115 N/A 3142118 unspecified role unspecified role neutral component neutral component burgl Burkholderia glumae Pseudomonas glumae N/A 3142119 unspecified role unspecified role neutral component neutral component binding region (19-353) binding region (19-353) sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lifo-lipa-2 3142116 N/A 3142118 unspecified role unspecified role neutral component neutral component burgl Burkholderia glumae Pseudomonas glumae N/A 3142119 unspecified role unspecified role neutral component neutral component binding region (19-353) binding region (19-353) sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false lifo-lipa-3 3142117 N/A 3142118 unspecified role unspecified role prey prey burgl Burkholderia glumae Pseudomonas glumae N/A 3142119 unspecified role unspecified role bait bait binding region (19-353) binding region (19-353) sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false