mpidb The Microbial Protein Interaction Database van_der_hen-2012-1 The histidine kinase PdhS controls cell cycle progression of the pathogenic alphaproteobacterium Brucella abortus. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Van der Henst C., Beaufay F., Mignolet J., Didembourg C., Colinet J., Hallet B., Letesson J.J., De Bolle X. J. Bacteriol. (0021-9193) 2012 Only protein-protein interactions imex curation mignolet-2010-1 PdhS, an old-pole-localized histidine kinase, recruits the fumarase FumC in Brucella abortus. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Mignolet J., Van der Henst C., Nicolas C., Deghelt M., Dotreppe D., Letesson J.J., De Bolle X. Brusella melitensis ORFeome J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation mignolet-2010-2 PdhS, an old-pole-localized histidine kinase, recruits the fumarase FumC in Brucella abortus. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Mignolet J., Van der Henst C., Nicolas C., Deghelt M., Dotreppe D., Letesson J.J., De Bolle X. Brusella melitensis ORFeome J. Bacteriol. (0021-9193) 2010 Only protein-protein interactions imex curation fumc_brume Fumarate hydratase class II fumC Aerobic fumarase Iron-independent fumarase BMEII1051 protein protein brume Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) MAATRTETDTFGPIDVPADRYWGAQTQRSLQNFRIGGERMPLPLVHALGVVKRAAAETNIALGKLDPVLGQVIAVAASEVIEGKLDDHFPLVVWQTGSGTQSNMNANEVISNRAIELLGGEMGSKKPIHPNDHVNMSQSSNDSFPTAIHIATAVETVNRLYPALEHLTKALKVKEEAFKDIIKIGRTHTQDATPVTLGQEFSGYRAALEYARHRLEQSLADVFLLAQGGTAVGTGLNAPVGFDKGFAEAVSEITGLSFKTAPNKFEALASHGAVLNFHGSLNALAADLFKIANDIRFLGSGPRSGLGELSLPENEPGSSIMPGKVNPTQAEAMTMVATQVFGNQTAVTVAASQGHFELNVFKPVIAYNVLQSIRLLSDTMVSFADHCVEGIEPNTARIKELLERSLMLVTALAPAIGYDNAARIAKTAHKNGTTLREEALASGLVSEEDYDRLVRAERMIAPQ divk_brume Polar-differentiation response regulator DivK divK BMEII0659 protein protein brume Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) MTKSVMIVEDNELNMKLFRDLIEASGYETIRTRSGLEALDLAREHHPDLILMDIQLPEVSGLEVTKWLKDDEELRHIPVIAVTAFAMKGDEERIRQGGCEAYISKPISVPRFIETIKSYLGDA divk_brua2 Polar-differentiation response regulator DivK divK BAB2_0628 protein protein brua2 brua2 MTKSVMIVEDNELNMKLFRDLIEASGYETIRTRSGLEALDLAREHHPDLILMDIQLPEVSGLEVTKWLKDDEELRHIPVIAVTAFAMKGDEERIRQGGCEAYISKPISVPRFIETIKSYLGDA pdhs_brua2 Cell-division control histidine kinase PdhS pdhS BAB1_1621 protein protein brua2 brua2 MSGSYPFIDIAALDSVREGFARGDAQLVLAHDLSTVLWVNGPGAKLFGYNRVEDLIEGQLDLPVATRRQIAAFSSENTSAPSAVAVRLGGGLRSELTHLHVSNIKLPDGVAALLVATQMPDNSAEAAISGLGDDSTHIALVDAVGKVVAASPRFALLDISASTLEDLIVEAGDATDRIVKRRIRTGSHSVPGAIARLTDTPALHLLCIVGDAPAQFQTAAEAVPLPDNAEAVLEEILPEQGDAPAQQAQKTHAEQPRPKTFAFDHDAPPARFIWKVGPDGTFSEISPNLAAVVGPNSADIVGRRFSDVANVFGFDTDGSIAALLLERDTWSGKRLLWPVEGTRLRVPVELAALPVYSRDREFLGFRGFGIVRPAEAEADPEEIGLALAGGIPQNRKPRKEPAETARMVGEDDVLALSEEVANDDQPAAVLPKPPLDITPTPGRRDSDKVISLLNSCAQEKVAADQAKFLKEKERATRPEGGLTKTERNAFREIAERLRKQGLANTRAESETPVSETSSIEPVEPTPPVKTRSEPIQPDETALLANLPVPVIIHSGDAIHYVNQALLDITGYESLDDIRSAGGVDVLFNSESDDGETRQSMLLRHADGSEEPVDAHLNAIAWRGGRALMLSLMPVTAADLPAPAELPAANDEEKQALEAHVEELKTILDTATDGVVLIDPEGRIRSMNHSASALFGYERDEAEGKFFSMLFAIESQRAAMDYLHGLSGNGVLSVLNDGREVIGREAKGGFIPLFMTIGKLPHTRGFCAVLRDITQWKRTEEELTNARKEAERASNQKTEFLARISHEIRTPLNAIIGFSELMADEKFGPIGNDRYRDYLRDINRSGNHVLALVNDLLDISKIEAGALDMQFEAVSLNDAIGEAIALMQPQANRERVIIRSSFQSNLPDIVADSRSIKQVALNLLSNAVRFTAPGGQVIVSTSYELNGDVVMRVRDTGIGMSKSEVEQALKPFRQINALERRKAESAKDWRNEGTGLGLPLTKAMVEANRAQFAIDSNPGQGTVVEIVFPPTRVLAD q2yij6_brua2 Fumarate hydratase class II fumC Aerobic fumarase Iron-independent fumarase BAB2_0186 protein protein brua2 brua2 MAATRTETDTFGPIDVPADRYWGAQTQRSLQNFRIGGERMPLPLVHALGVVKRAAAETNIALGKLDPVLGQVIAVAASEVIEGKLDDHFPLVVWQTGSGTQSNMNANEVISNRAIELLGGEMGSKKPIHPNDHVNMSQSSNDSFPTAIHIATAVETVNRLYPALEHLTKALKVKEEAFKDIIKIGRTHTQDATPVTLGQEFSGYRAALEYARHRLEQSLADVFLLAQGGTAVGTGLNAPVGFDKGFAEAVSEITGLSFKTAPNKFEALASHGAVLNFHGSLNALAADLFKIANDIRFLGSGPRSGLGELSLPENEPGSSIMPGKVNPTQAEAMTMVATQVFGNQTAVTVAASQGHFELNVFKPVIAYNVLQSIRLLSDTMVSFADHCVEGIEPNTARIKELLERSLMLVTALAPAIGYDNAARIAKTAHKNGTTLREEALASGLVSEEDYDRLVRAERMIAPQ pdhs-divk-1 1340053 not-defined-770055085 1340054 unspecified role unspecified role prey prey not-defined-188238254 1340055 unspecified role unspecified role bait bait q2yrb4-638-1035 Q2YRB4-638-1035 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 4 pdhs-fumc-1 1340053 not-defined-1027876265 1340056 unspecified role unspecified role prey prey not-defined-509476121 1340055 unspecified role unspecified role bait bait q2yrb4-210-531 Q2YRB4-210-531 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 4 pdhs-pdhs-1 1340053 not-defined-1446919568 1340055 unspecified role unspecified role prey prey not-defined-1825353263 1340055 unspecified role unspecified role bait bait region Q2YRB4-210-531 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false figure 4 pdhs-divk-2 1337353 n/a 1337355 unspecified role unspecified role prey prey not-defined-132021330 1340055 unspecified role unspecified role bait bait physical association physical association false false false pdhs-fumc-2 1337353 n/a 1337357 unspecified role unspecified role prey prey not-defined-570990175 1340055 unspecified role unspecified role bait bait physical association physical association false false false divk-pdhs 1337354 n/a 1337355 unspecified role unspecified role bait bait not-defined-132021330 1340055 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1A fumc-pdhs 1337354 n/a 1337357 unspecified role unspecified role bait bait not-defined-570990175 1340055 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 1A HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html http://www.agbase.msstate.edu/hpi/main.html del-2016-1 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Del Giudice MG., Döhmer PH., Spera JM., Laporte FT., Marchesini MI., Czibener C., Ugalde JE. jugalde@iibintech.com.ar The Journal of biological chemistry 2016 imex curation Only protein-protein interactions imex curation del-2016-3 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Del Giudice MG., Döhmer PH., Spera JM., Laporte FT., Marchesini MI., Czibener C., Ugalde JE. jugalde@iibintech.com.ar The Journal of biological chemistry 2016 imex curation Only protein-protein interactions imex curation del-2016-2 ecoli Escherichia coli (strain K12) adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Del Giudice MG., Döhmer PH., Spera JM., Laporte FT., Marchesini MI., Czibener C., Ugalde JE. jugalde@iibintech.com.ar The Journal of biological chemistry 2016 imex curation Only protein-protein interactions imex curation guidolin-2015-1 ecoli Escherichia coli (strain K12) adenylate cyclase adenylate cyclase complementation bacterial two-hybrid bacterial two-hybrid adenylate cyclase predetermined predetermined participant predetermined Guidolin LS., Morrone Seijo SM., Guaimas FF., Comerci DJ., Ciocchini AE. dcomerci@iibintech.com.ar,aciocchini@iibintech.com.ar Diego J. Comerci, dcomerci@iibintech.com.ar, or Andrés E. Ciocchini, aciocchini@iibintech.com.ar. Journal of bacteriology 2015 imex curation Only protein-protein interactions imex curation guidolin-2015-2 ecoli Escherichia coli (strain K12) anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Guidolin LS., Morrone Seijo SM., Guaimas FF., Comerci DJ., Ciocchini AE. dcomerci@iibintech.com.ar,aciocchini@iibintech.com.ar Diego J. Comerci, dcomerci@iibintech.com.ar, or Andrés E. Ciocchini, aciocchini@iibintech.com.ar. Journal of bacteriology 2015 imex curation Only protein-protein interactions imex curation guidolin-2015-3 brua2 brua2 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Guidolin LS., Morrone Seijo SM., Guaimas FF., Comerci DJ., Ciocchini AE. dcomerci@iibintech.com.ar,aciocchini@iibintech.com.ar Diego J. Comerci, dcomerci@iibintech.com.ar, or Andrés E. Ciocchini, aciocchini@iibintech.com.ar. Journal of bacteriology 2015 imex curation Only protein-protein interactions imex curation q2yld2_brua2 BAB2_0123 protein protein brua2 brua2 MSLLLANVLSTLMALASLAALIVVGRKANETIRLGKVLALRVAAASNGLERAVKAIRNERTDLTDASAKLDARLAETQRVRREMDEAIEELNRLRKALSRDMRQARILAETPPPRQEKTPSAAEQPLPADKNALPVFVRRAVKKATVELAGQA q2ykk5_brua2 BAB2_0654 protein protein brua2 brua2 MDAMLARNIIVMPVELGPWRAALDKEDGECNYLDSDFEAIAKEALRGLDLGVYFHPVLTGIGQGATIAYAAASDSPDATIAGAVSLDPTPARTRLPSCTEKAEAIKGPDGGFTYSVNAALPAPALLISPPGSPVNNPVEARRKNIAVLQTAPDFSARMKMAVDNVVAMADQDAKNEALPIIDLPAKGGNADMVAVFYSGDGGWRDIDKSIGEWLQAHGVHVIGVDSLRYFWSERTPEEIANDTTAMIKKADPTGKLPVAVLGYSFGADTFPFAWKYLDPSIQDRTRMVGLLGVETTTTFQVSIEGWLGMNGDKDVVPAIASIPLDRVVCVYGQEEDDTACTADELKGMDTMKLPGGHHFDENYEPIAAALLDKMRSRAGLPPRPAR q2ys16_brua2 BAB1_1492 protein protein brua2 brua2 MMPVIRLNDATFADLSVLKTWYGTKTPSETIDRIVREAMEQLDMERDDAAEEVTVTTSDGAMHFDAAPGLAFTKPLAASIDGKALHSPCWSALLLTMIAQVKTKGLSGDKLVRELAIPAKVERYDEEGFKFRPDLGISVQGQSASDCWKEVERLSKKWAIPVSVKFWWKQNPKAQYPGKTGILRSGPASA virb5_brua2 Type IV secretion system protein virB5 virB5 BAB2_0064 protein protein brua2 brua2 MKKIILSFAFALTVTSTAHAQLPVTDAGSIAQNLANHLEQMVKFAQQIEQLKQQFEQQKMQFDALTGNRGLGDILRDPTLRSYLPHNWRDLYEAVMSGGYLAAAGETANLLRKSQVYDPCASISDKDQRIACEAKVVKPVQDKVMTSKAYDATDKRLQEIESLMQEINKTGDPKAIAELQGRIESENAMIQNEDTRLRLYQQMAEAQDKLLDERQHELDAKDNARRGYPQPKALEAAY virb8_brua2 Type IV secretion system protein virB8 virB8 BAB2_0061 protein protein brua2 brua2 MFGRKQSPQKSVKNGQGNAPSVYDEALNWEAAHVRLVEKSERRAWKIAGAFGTITVLLGIGIAGMLPLKQHVPYLVRVNAQTGAPDILTSLDEKSVSYDTVMDKYWLSQYVIARETYDWYTLQKDYETVGMLSSPSEGQSYASQFQGDKALDKQYGSNVRTSVTIVSIVPNGKGIGTVRFAKTTKRTNETGDGETTHWIATIGYQYVNPSLMSESARLTNPLGFNVTSYRVDPEMGVVQ q2yrc8_brua2 BAB1_1718 protein protein brua2 brua2 MVPQGRDTRIDVFRALALLTIFINHVPGTIYEHFTHKNLGFSDSAEAFVLISGMAVALAYGSKFVSGNRLVLSLKMWRRASVLYITHIMTTMATIAIFAAAAIFLKRSELLTQINIAPLIKKPVEGLFGLVTLGHQLGYNNILSMYAVMLILTPILLFLARISLPLMLGVSGVVWFLSGLYHVAPVNYPTEGVWFINPLSWQFLFAIGIAGVMHVRGGGQLPKHPLLIGLAVGYLVLALAWVRIPLWSINISMGLPMVITGFDKTFLSAPRLLHVLAMAYLIAVVPTLNSIARTAPEHPLAVLGKHSLPVFVAGTLLAMVAQVMKLVNPGGLPYDTLLIATGIVMQFALAYYLEWLPTIGWGKKGAVQAKPAPAAEPVVIQVSANPEPAMQRY gcn4_yeast General control transcription factor GCN4 ARG9 GCN4 AAS3 Amino acid biosynthesis regulatory protein AAS101 General control protein GCN4 YEL009C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSEYQPSLFALNPMGFSPLDGSKSTNENVSASTSTAKPMVGQLIFDKFIKTEEDPIIKQDTPSNLDFDFALPQTATAPDAKTVLPIPELDDAVVESFFSSSTDSTPMFEYENLEDNSKEWTSLFDNDIPVTTDDVSLADKAIESTEEVSLVPSNLEVSTTSFLPTPVLEDAKLTQTRKVKKPNSVVKKSHHVGKDDESRLDHLGVVAYNRKQRSIPLSPIVPESSDPAALKRARNTEAARRSRARKLQRMKQLEDKVEELLSKNYHLENEVARLKKLVGER 2ED1B8E35D509578 q2ynv7_brua2 BAB1_0108 protein protein brua2 brua2 MPRIFALAWLYVAHTDSNFSLKMLTAMVKGFQTVEPLKIGELWALPSAVRYFLIENARRLAMRVDRAREMRNLANTAADRIVVAAEGAELDGILAQYASAACDSTFATHLLYRLRSSSTDTTAAASWLERILEQDGSNPEDAIIEEHARQSTGGVTMGNVIRSLKSIDDVDWETWFETVNEVDAILRDHSDFELLDFRSRNAYRVTIEKLARYSDMSEIDITWAALKLAEDGSRAEKAGHPEAEGKGAISYYLSGEGRRDLEKVCGYRAPFGVKALRLYRGLGLWGLFVPTAIFTVLTLLLVNYCMMRAGLSTDLRTLFTLLAIFPAMDASYSLFNVLVPWFVQPTRLIGFEYKDGLPPEARTLVAVPTFITSRDSIDEQIRNLEVHYLTNPRGEIYFALVSDWTDASQEIVPADMEIYEYARDEIAKLNEHYTGDDQPRFFLLHRRRLFNEKQGCWMGWERKRGKLHELNLLLRGDQDTSFFPADERLPKDIKYVMTLDADTRLTPEAVTHLAGKLNHPMNRPVIDEKTGKVVRGYGILQPRVTPSLTTGDEASFLQRVFSVNRGIDPYVFAVSDTYQDLLGEGTFTGKGLYDIDAFEAALKGRVPENTLLSHDLLEGGYARSALVSDVEVVEDYPTGYNVDVSRHHRWARGDWQLLPWLFSTGRGVSAITRWKMVDNLRRSLNPILWLIAAIVGWSVLPLGAAAVWQGFLILSMFVAPTFGIVTNLIPASMDYSLKGHAQSVLTDIALGTADVALRTTFIAHSAFLMADAIARTIYRLFISRRNLLEWRTAAQARTSPDTLLFYFQLMWPAMAIAGLAIFFPLAAESHAALIAAPFVVIWFASPLIAWLVSRSAKPQDTLEVLSSDKADLRRYARRTWRYFAEFANEENNHLPPDNFQEDPAPIVAQRTSPTNIGVYLLSVIAARDFGWIGFDETLDRVEATLGSLDKMEKYQGHLYNWYETNTLNPMRPLYVSAVDSGNLAGHLVTLSSALEEWAEAPSVYVQGDLDGIIDVNDILEETIARIPDDRRVLRPLRRRLEERIANFRRAVTSIKDEPETASFRTINLSLFASDIVRLMDELDSEVETVASAEAREWARILVDTCKAHTDDAIGEHDTDALRERLRDLATRARQLAFDMQFGFLERKERRLLSIGFRVQENELDESCYDLLASEARLASLFAIAKGDVPVEHWFKLGRLLVPVGWKGALLSWSGSMFEYLMPPLVMSEPLGSLLDQTNKLVVQRQIDYATSRDLPWGISEAAFNARDAHLNYQYSNFGVPNLGLQRGLSRNAVIAPYASLLAAQYQPAAAVANLKRLAKLGALGRYGFHDSVDFTPSRIREGETCAVVKNYYAHHHGMSIIAVNNVIFEGRMRERFHNDPVIEAAELLLQEKAPREIPMIYAKTENPMRVDSGGFDDAPMRIIDKPFRAPRTTHMMSNGQYALMVTANGSGYSRWHNWDITRFHADASEDMQGTFLFLRDMESGYWWSATGEPVRNPDEETKTVFTEDKAEFYKTAGDIKTVMEVIVASEADGEGRRLDIINTSPRDRMIEVTSYAELVLNDSDSDAAHPAFAKMFVETEIADSGSTIYAKRRKRAPSAPDIHAAHFVTDLSGALREIEVETDRRAFIGRGRSLRNAAAFDPGAKFTGSQGCVLDPIVSVRTRVRVPAHKKVSLVFWTFAGGSRDAVEHAVTMHRHPETFAREYSLSWTSSQVQLYHIGIKPAEAADYQKVAAYLLYPERTLRQPPETIASGLGKQSDLWPMSISGDYPIFALRIDNEADLDVLRGVLRAQEYWRSKGLIVDVVAVNERAFSYAQDTQRAIDWIVEGFRARGGGQPHIFAVRRDQMSEESYNTLLASARIVMHAQNGSLAEQLRRSEEITVDLAARSDARTATNGQASVQGLGAAGLAPVAIERGAEERRPVVKPPVDRPRPSGDDLRFWNGYGGFAEDGSYVVRINGRTSTPHPWINVIANPSFGFHVSAEGSAFTWAGNSRDYQLTPWANDPVTNRPGEAIYILDRETGRSFAPTANVLRDEAVTYEARHGHGYSAFSAQHGELALELTHIVDAEKPVRLSRLTITNKGRSKRKLRVYGYVEWVLGNARARNVPFIVPSQDEELGALFAGNPYHPDKSGQVAFFAASEKPQSVTADRGEFIGTAGSVDRPEIVLAGKVLSGTVEAGRDPCAALALDIEVGPGEAREIVFLLGNTADQKQAKALIADMRTASFEEKLVAARHQWESFFNRVQVKTPDPAFDLMVNGWLPYQAVACRIWARAAFYQVSGAFGFRDQLQDTLSLLLVDPSLAREQILNAASRQFREGDVQHWWLPATGAGVRTLISDDVVWLGYGASLYVETTGDRFILDEKLPFLEGRKLEEGEHDAFYEPEISKETASLYEHCALALDLAVARTGKHGLPLILGGDWNDGMNLVGVKGKGESVWLGWFLAYTLKAFIPLAEARKDQKRADLWKAHVESLTAALDRDGWDGEWYRRGFYDNGRPLGSKNSDECQIDAIAQSWSVLSGVADPKRAKKAMASLEKYLLDEDNALLRLFTPPFDVTPQEPGYIKGYPPGVRENGGQYTHGATWAILALARMGKAAEAWRLFSLISPVSHGRNPDVYRVEPYVIAADIYSVAPRCGQGGWTWYTGSAGWFYRVATEGILGVTKRGDRLHFQPALPPEWEGYEAELRFGEALYRVKVVAGAKAGTIRLNGRKLAKPDEGVALKPYGEHEIVVELPKQ ndva_brua2 Beta-(1-->2)glucan export ATP-binding/permease protein NdvA ndvA cgt BAB1_1017 protein protein brua2 brua2 MSLLKIYWRAMQYLAVERTATITMCVASVLVALVTLAEPVLFGRVIQSISDKGDIFSPLLMWAALGGFNIMAAVFVARGADRLAHRRRLGVMIDSYERLITMPLAWHQKRGTSNALHTLIRATDSLFTLWLEFMRQHLTTVVALATLIPVAMTMDMRMSLVLIVLGVIYVMIGQLVMRKTKDGQAAVEKHHHKLFEHVSDTISNVSVVQSYNRIASETQALRDYAKNLENAQFPVLNWWALASGLNRMASTFSMVVVLVLGAYFVTKGQMRVGDVIAFIGFAQLMIGRLDQISAFINQTVTARAKLEEFFQMEDATADRQEPENVADLNDVKGDIVFDNVTYEFPNSGQGVYDVSFEVKPGQTVAIVGPTGAGKTTLINLLQRVFDPAAGRIMIDGTDTRTVSRRSLRHAIATVFQDAGLFNRSVEDNIRVGRANATHEEVHAAAKAAAAHDFILAKSEGYDTFVGERGSQLSGGERQRLAIARAILKDSPILVLDEATSALDVETEEKVTQAVDELSHNRTTFIIAHRLSTVRSADLVLFMDKGHLVESGSFNELAERGGRFSDLLRAGGLKLEDKQPKQPVVEGSNVMPFPVKGAVA virj-sepa-1 2843386 N/A 2843389 unspecified role unspecified role prey prey purified purified c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 2843390 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4A virj-bpe123-1 2843386 N/A 2843391 unspecified role unspecified role prey prey purified purified c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) N/A 2843390 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4A virj-virb5-virb8-1 2843387 N/A 2843392 unspecified role unspecified role prey prey N/A 2843393 unspecified role unspecified role prey prey N/A 2843390 unspecified role unspecified role bait bait c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false 5 sepa-virb5-virb8-1 2843387 N/A 2843392 unspecified role unspecified role prey prey N/A 2843393 unspecified role unspecified role prey prey N/A 2843389 unspecified role unspecified role bait bait c terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false 5 virj-sepa-2 2843388 N/A 2843389 unspecified role unspecified role bait bait N/A 2843390 unspecified role unspecified role prey prey physical association physical association false false false 4B cgs-cgs-1 1900127 N/A 1900130 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 1900130 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1A and 3 cgt-cgt-1 1900127 N/A 1900131 unspecified role unspecified role bait bait N/A 1900131 unspecified role unspecified role prey prey physical association physical association false false false 1A and 3 cgm-cgm-1 1900127 N/A 1900132 unspecified role unspecified role prey prey N/A 1900132 unspecified role unspecified role bait bait physical association physical association false false false 1A and 3 cgt-cgm-1 1900127 N/A 1900131 unspecified role unspecified role bait bait N/A 1900132 unspecified role unspecified role prey prey physical association physical association false false false 1B cgs-cgt-1 1900127 N/A 1900130 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false N/A 1900131 unspecified role unspecified role prey prey physical association physical association false false false 1B and 3 cgs-cgm-1 1900127 N/A 1900132 unspecified role unspecified role bait bait N/A 1900130 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B and 3 cgs-cgt-2 1900127 N/A 1900131 unspecified role unspecified role bait bait N/A 1900130 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3 cgs-cgm-2 1900127 N/A 1900132 unspecified role unspecified role prey prey N/A 1900130 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3 zip-zip-1 1900127 N/A 1900133 unspecified role unspecified role bait bait N/A 1900133 unspecified role unspecified role prey prey physical association physical association false false false 1A cgs-cgt-3 1900128 N/A 1900131 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1900130 unspecified role unspecified role bait bait his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2A cgt-cgm-2 1900128 N/A 1900131 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1900132 unspecified role unspecified role prey prey T18 fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2B cgs-cgm-cgt-1 1900128 N/A 1900130 unspecified role unspecified role bait bait his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1900131 unspecified role unspecified role bait bait flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1900132 unspecified role unspecified role prey prey t18 fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false association association false false false 2C cgt-cgs-1 1900129 N/A 1900131 unspecified role unspecified role neutral component neutral component ecfp cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1900130 unspecified role unspecified role neutral component neutral component eyfp egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 5A cgt-cgm-3 1900129 N/A 1900131 unspecified role unspecified role neutral component neutral component ecfp cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 1900132 unspecified role unspecified role neutral component neutral component mcherry mcherry mcherry fluorescent protein tag mcherry undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false 5C