DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ chen-2007-30 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Chen J., Hua G., Jurat-Fuentes JL., Abdullah MA., Adang MJ. DIP original experiment: DIP-113930X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Synergism of Bacillus thuringiensis toxins by a fragment of a toxin-binding cadherin. Chen J., Hua G., Jurat-Fuentes JL., Abdullah MA., Adang MJ. Only protein-protein interactions imex curation chen-2007-31 in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Chen J., Hua G., Jurat-Fuentes JL., Abdullah MA., Adang MJ. DIP original experiment: DIP-113931X Proceedings of the National Academy of Sciences of the United States of America 2007 IMEx Synergism of Bacillus thuringiensis toxins by a fragment of a toxin-binding cadherin. Chen J., Hua G., Jurat-Fuentes JL., Abdullah MA., Adang MJ. Only protein-protein interactions imex curation cr1ab_bactb Pesticidal crystal protein Cry1Ab cry1Ab bt2 cry-1-2 cry1A(b) cryIA(b) cryIC1 130 kDa crystal protein Crystaline entomocidal protoxin Insecticidal delta-endotoxin CryIA(b) protein protein bactb Bacillus thuringiensis subsp. berliner MDNNPNINECIPYNCLSNPEVEVLGGERIETGYTPIDISLSLTQFLLSEFVPGAGFVLGLVDIIWGIFGPSQWDAFLVQIEQLINQRIEEFARNQAISRLEGLSNLYQIYAESFREWEADPTNPALREEMRIQFNDMNSALTTAIPLFAVQNYQVPLLSVYVQAANLHLSVLRDVSVFGQRWGFDAATINSRYNDLTRLIGNYTDHAVRWYNTGLERVWGPDSRDWIRYNQFRRELTLTVLDIVSLFPNYDSRTYPIRTVSQLTREIYTNPVLENFDGSFRGSAQGIEGSIRSPHLMDILNSITIYTDAHRGEYYWSGHQIMASPVGFSGPEFTFPLYGTMGNAAPQQRIVAQLGQGVYRTLSSTLYRRPFNIGINNQQLSVLDGTEFAYGTSSNLPSAVYRKSGTVDSLDEIPPQNNNVPPRQGFSHRLSHVSMFRSGFSNSSVSIIRAPMFSWIHRSAEFNNIIPSSQITQIPLTKSTNLGSGTSVVKGPGFTGGDILRRTSPGQISTLRVNITAPLSQRYRVRIRYASTTNLQFHTSIDGRPINQGNFSATMSSGSNLQSGSFRTVGFTTPFNFSNGSSVFTLSAHVFNSGNEVYIDRIEFVPAEVTFEAEYDLERAQKAVNELFTSSNQIGLKTDVTDYHIDQVSNLVECLSDEFCLDEKKELSEKVKHAKRLSDERNLLQDPNFRGINRQLDRGWRGSTDITIQGGDDVFKENYVTLLGTFDECYPTYLYQKIDESKLKAYTRYQLRGYIEDSQDLEIYLIRYNAKHETVNVPGTGSLWPLSAPSPIGKCAHHSHHFSLDIDVGCTDLNEDLGVWVIFKIKTQDGHARLGNLEFLEEKPLVGEALARVKRAEKKWRDKREKLEWETNIVYKEAKESVDALFVNSQYDRLQADTNIAMIHAADKRVHSIREAYLPELSVIPGVNAAIFEELEGRIFTAFSLYDARNVIKNGDFNNGLSCWNVKGHVDVEEQNNHRSVLVVPEWEAEVSQEVRVCPGRGYILRVTAYKEGYGEGCVTIHEIENNTDELKFSNCVEEEVYPNNTVTCNDYTATQEEYEGTYTSRNRGYDGAYESNSSVPADYASAYEEKAYTDGRRDNPCESNRGYGDYTPLPAGYVTKELEYFPETDKVWIEIGETEGTFIVDSVELLLMEE q8mzk3_manse protein protein manse Manduca sexta (Tobacco hawkmoth) Tobacco hornworm Tobacco hawkmoth MAVDVRIAAFLLVFIAPAVLAQERCGYMTAIPRLPRPDNLPVLNFEGQTWSQRPLLPAPERDDLCMDAYHVITANLGTQVIYMDEEIEDEITIAILNYNGPSTPFIELPFLSGSYNLLMPVIRRVDNGEWHLIITQRQDYELPGMQQYMFNVRVDGQSLVAGVSLAIVNIDDNAPIIQNFEPCRVPELGEPGLTECTYQVSDADGRISTEFMTFRIDSVRGDEETFYIERTNIPNQWMWLNMTLGVNTSLNFVTSPLHIFSVTALDSLPNTHTVTMMVQVANVNSRPPRWLEIFAVQQFEEKSYQNFTVRAIDGDTEINMPINYRLITNEEDTFFSIEALPGGKSGAIFHVSPIDRDTLQREVFPLTIVAYKYDEEAFSTSTNVVIIVTDINDQRPEPIHKEYRLAIMEETPMTLNFDKEFGFHDKDLGQNAQYTVRLESVDPPGAAEAFYIAPEVGYQRQTFIMGTLNHSMLDYEVPEFQSITIRVVATDNNDTRHVGVALVHIDLINWNDEQPIFEHAVQTVTFDETEGEGFFVAKAVAHDRDIGDVVEHTLLGNAVNFLTIDKLTGDIRVSANDSFNYHRESELFVQVRATDTLGEPFHTTTSQLVIRLNDINNTPPTLRLPRGSPQVEENVPDGHVITQELRATDPDTTADLRFEINWDTSFATKQGRQANPDEFRNCVEIETIFPEINNRGLAIGRVVAREIRHNVTIDYEEFEVLSLTVRVRDLNTVYGDDYDESMLTITIIDMNDNAPVWVEGTLEQNFRVREMSAGGLVVGSVRADDIDGPLYNQVRYTIFPREDTDKDLIMIDFLTGQISVNTSGAIDADTPPRFHLYYTVVASDRCSTEDPADCPPDPTYWETEGNITIHITDTNNKVPQAETTKFDTVVYIYENATHLDEVVTLIASDLDRDEIYHTVSYVINYAVNPRLMNFFSVNRETGLVYVDYETQGSGEVLDRDGDEPTHRIFFNLIDNFMGEGEGNRNQNDTEVLVILLDVNDNAPELPPPSELSWTISENLKQGVRLEPHIFAPDRDEPDTDNSRVGYEILNLSTERDIEVPELFVMIQIANVTGELETAMDLKGYWGTYAIHIRAFDHGIPQMSMNETYELIIHPFNYYAPEFVFPTNDAVIRLARERAVINGVLATVNGEFLERISATDPDGLHAGVVTFQVVGDEESQRYFQVVNDGANLGSLRLLQAVPEEIREFRITIRATDQGTDPGPLSTDMTFRVVFVPTQGEPRFASSEHAVAFIEKSAGMEESHQLPLAQDIKNHLCEDDCHSIYYRIIDGNSEGHFGLDPVRNRLFLKKELIREQSASHTLQVAASNSPDGGIPLPASILTVTVTVREADPRPVFMRELYTAGISTADSIGRELLRLHATQSEGAAITYAIDYDTMVVDPSLEAVRQSAFVLNAQTGVLTLNIQPTATMHGLFKFEVTATDTAGAQDRTDVTVYVVSSQNRVYFVFVNTLQQVEDNRDFIADTFSAGFNMTCNIDQVVPANDPVTGVALEHSTQMRGHFIRDNVPVLADEIEQIRSDLVLLSSIQTTLAARSLVLQDLLTNSSPDSAPDSSLTVYVLASLSAVLGFMCLVLLLTFIIRTRALNRRLEALSMTKYGSLDSGLNRAGIAAPGTNKHTVEGSNPIFNEAIKTPDLDAISEGSNDSDLIGIEDLPHFGNVFMDPEVNEKANGYPEVANHNNNFAFNPTPFSPEFVNGQFRKI q8mzk3_manse-cry1ab-1 3160389 N/A 3160391 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3160392 unspecified role unspecified role bait bait Region 1416-1423 Region 1416-1423 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false q8mzk3_manse-cry1ab-2 3160390 N/A 3160391 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3160392 unspecified role unspecified role bait bait Region 1416-1423 Region 1416-1423 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false