IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ sevcik-1998-1 Recognition of RNase Sa by the inhibitor barstar: structure of the complex at 1.7 A resolution. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Sevcik J., Urbanikova L., Dauter Z., Wilson KS. PDBe - Data obtained from the Protein Data Bank Europe Acta Crystallogr. D Biol. Crystallogr. (0907-4449) 1998 radisky-2004-1 Binding, proteolytic, and crystallographic analyses of mutations at the protease-inhibitor interface of the subtilisin BPN'/chymotrypsin inhibitor 2 complex. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Radisky ES., Kwan G., Karen Lu CJ., Koshland DE. PDBe - Data obtained from the Protein Data Bank Europe Biochemistry (0006-2960) 2004 vaughan-1999-1 Structural response to mutation at a protein-protein interface. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Vaughan CK., Buckle AM., Fersht AR. PDBe - Data obtained from the Protein Data Bank Europe J. Mol. Biol. (0022-2836) 1999 bars_bacam Barstar Ribonuclease inhibitor protein protein bacam Bacillus amyloliquefaciens MKKAVINGEQIRSISDLHQTLKKELALPEYYGENLDALWDCLTGWVEYPLVLEWRQFEQSKQLTENGAESVLQVFREAKAEGCDITIILS rnsa_kitau Guanyl-specific ribonuclease Sa rnaSA protein protein strau Streptomyces aureofaciens DVSGTVCLSALPPEATDTLNLIASDGPFPYSQDGVVFQNRESVLPTQSYGYYHEYTVITPGARTRGTRRIITGEATQEDYYTGDHYATFSLIDQTC rnbr_bacam Ribonuclease Barnase RNase Ba protein protein bacam Bacillus amyloliquefaciens MMKMEGIALKKRLSWISVCLLVLVSAAGMLFSTAAKTETSSHKAHTEAQVINTFDGVADYLQTYHKLPDNYITKSEAQALGWVASKGNLADVAPGKSIGGDIFSNREGKLPGKSGRTWREADINYTSGFRNSDRILYSSDWLIYKTTDHYQTFTKIR subt_bacam Subtilisin BPN' apr Subtilisin Novo Subtilisin DFE Alkaline protease protein protein bacam Bacillus amyloliquefaciens MRGKKVWISLLFALALIFTMAFGSTSSAQAAGKSNGEKKYIVGFKQTMSTMSAAKKKDVISEKGGKVQKQFKYVDAASATLNEKAVKELKKDPSVAYVEEDHVAHAYAQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETNPFQDNNSHGTHVAGTVAALNNSIGVLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGTSGSSSTVGYPGKYPSVIAVGAVDSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPNWTNTQVRSSLENTTTKLGDSFYYGKGLINVQAAAQ q40059_horvu Ica-2 protein protein horvu Hordeum vulgare (Barley) Barley MSSMEKKPEGVNIGAGDRQNQKTEWPELVGKSVEEAKKVILQDKPAAQIIVLPVGTIVTMEYRIDRVRLFVDRLDNIAQVPRVG bars-rnsa-1 Ribonuclease sa complex with barstar 511594 N/A 511595 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 511596 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false dimeric, multimeric working 84.00%, free 81.00% 1.7A apr-ica_2-1 Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 515166 N/A 515167 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false bacsu Bacillus subtilis Stoichiometry: 1.0 N/A 515168 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false dimeric, multimeric working 84.900%, free 81.800% 1.7A apr-ica_2-2 Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 m59k mutant 515166 N/A 515167 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false bacsu Bacillus subtilis Stoichiometry: 1.0 N/A 515168 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false dimeric, multimeric working 83.400%, free 81.600% 1.57A apr-ica_2-3 Crystal structure of the complex of subtilsin bpn'with chymotrypsin inhibitor 2 m59g mutant 515166 N/A 515168 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 515167 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false bacsu Bacillus subtilis Stoichiometry: 1.0 direct interaction direct interaction false false false dimeric, multimeric working 84.200%, free 81.700% 1.7A apr-ica_2-4 Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 m59a mutant 515166 N/A 515168 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 515167 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false bacsu Bacillus subtilis Stoichiometry: 1.0 direct interaction direct interaction false false false dimeric, multimeric working 83.900%, free 82.100% 1.45A apr-ica_2-5 Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 m59y mutant 515166 N/A 515167 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false bacsu Bacillus subtilis Stoichiometry: 1.0 N/A 515168 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false dimeric, multimeric working 84.500%, free 82.500% 1.59A apr-ica_2-6 Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 m59f mutant 515166 N/A 515167 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false bacsu Bacillus subtilis Stoichiometry: 1.0 N/A 515168 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false dimeric, multimeric working 85.100%, free 82.600% 1.67A apr-ica_2-7 Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 y61a mutant 515166 N/A 515168 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 515167 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false bacsu Bacillus subtilis Stoichiometry: 1.0 direct interaction direct interaction false false false dimeric, multimeric working 84.500%, free 81.800% 1.68A apr-ica_2-8 Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 m59k, in ph 9 cryosoak 515166 N/A 515167 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false bacsu Bacillus subtilis Stoichiometry: 1.0 N/A 515168 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false dimeric, multimeric working 83.200%, free 81.700% 1.3A bars-rnbr-1 Structural response to mutation at a protein-protein interface 511719 N/A 511596 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 3.0 N/A 511721 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 3.0 direct interaction direct interaction false false false dimeric, multimeric working 80.600%, free 75.100% 1.82A MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint radisky-2002-1 A clogged gutter mechanism for protease inhibitors. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Radisky ES., Koshland DE. PDBe - Data obtained from the Protein Data Bank Europe Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2002 heinz-1991-1 Refined crystal structures of subtilisin novo in complex with wild-type and two mutant eglins. Comparison with other serine proteinase inhibitor complexes. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Heinz DW., Priestle JP., Rahuel J., Wilson KS., Grütter MG. J. Mol. Biol. (0022-2836) 1991 takeuchi-1991-1 Molecular recognition at the active site of subtilisin BPN': crystallographic studies using genetically engineered proteinaceous inhibitor SSI (Streptomyces subtilisin inhibitor). in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Takeuchi Y., Noguchi S., Satow Y., Kojima S., Kumagai I., Miura K., Nakamura KT., Mitsui Y. Protein Eng. (0269-2139) 1991 subt_bacam Subtilisin BPN' apr Subtilisin Novo Subtilisin DFE Alkaline protease protein protein bacam Bacillus amyloliquefaciens MRGKKVWISLLFALALIFTMAFGSTSSAQAAGKSNGEKKYIVGFKQTMSTMSAAKKKDVISEKGGKVQKQFKYVDAASATLNEKAVKELKKDPSVAYVEEDHVAHAYAQSVPYGVSQIKAPALHSQGYTGSNVKVAVIDSGIDSSHPDLKVAGGASMVPSETNPFQDNNSHGTHVAGTVAALNNSIGVLGVAPSASLYAVKVLGADGSGQYSWIINGIEWAIANNMDVINMSLGGPSGSAALKAAVDKAVASGVVVVAAAGNEGTSGSSSTVGYPGKYPSVIAVGAVDSSNQRASFSSVGPELDVMAPGVSIQSTLPGNKYGAYNGTSMASPHVAGAAALILSKHPNWTNTQVRSSLENTTTKLGDSFYYGKGLINVQAAAQ ici2_horvu Subtilisin-chymotrypsin inhibitor-2A protein protein horvu Hordeum vulgare (Barley) Barley MSSVEKKPEGVNTGAGDRHNLKTEWPELVGKSVEEAKKVILQDKPEAQIIVLPVGTIVTMEYRIDRVRLFVDKLDNIAQVPRVG icic_hirme Eglin C protein protein medicinal leech Hirudo medicinalis TEFGSELKSFPEVVGKTVDQAREYFTLHYPQYDVYFLPEGSPVTLDLRYNRVRVFYNPGTNVVNHVPHVG mint Inhibits both elastase and cathepsin G ssi_strao Subtilisin inhibitor ssi SSI type protein protein strao Streptomyces albogriseolus MRNTGAGPSPSVSRPPPSAAPLSGAALAAPGDAPSALYAPSALVLTVGKGVSATTAAPERAVTLTCAPGPSGTHPAAGSACADLAAVGGDLNALTRGEDVMCPMVYDPVLLTVDGVWQGKRVSYERVFSNECEMNAHGSSVFAF mint Strong inhibitor of bacterial serine proteases such as subtilisin apr-ici2-1 Crystal structure of the complex of subtilisin bpn' with chymotrypsin inhibitor 2 at 1.5 angstrom resolution 99458 N/A 99459 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false bacsu Bacillus subtilis Stoichiometry: 1.0 N/A 99460 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false dimeric, multimeric working 83.100%, free 81.200% 1.5A apr-icic 100494 icic_hirme 100495 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role unspecified role unspecified role purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 subt_bacam 99459 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role unspecified role unspecified role purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 direct interaction direct interaction false false false In the xray complex 1sbn, P00782 is chain E and P01051 is chain I Resolution 2.100 mint apr-ssi 100734 ssi_strao 100735 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role unspecified role unspecified role purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 subt_bacam 99459 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role unspecified role unspecified role purified purified tagged molecule tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 direct interaction direct interaction false false false In the xray complex 3sic, P00782 is chain E and P01006 is chain I Resolution 1.800 mint