DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ schwarz_linek-2001-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Schwarz-Linek U., Plevin MJ., Pickford AR., Höök M., Campbell ID., Potts JR. B3 binding region is taken from PMID:12736686 and PMID:8319691, as referenced in the paper, rather than the range given in the paper. jenp@bioch.ox.ac.uk FEBS letters 2001 imex curation Only protein-protein interactions imex curation schwarz_linek-2001-2 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Schwarz-Linek U., Plevin MJ., Pickford AR., Höök M., Campbell ID., Potts JR. jenp@bioch.ox.ac.uk FEBS letters 2001 imex curation Only protein-protein interactions imex curation schwarz_linek-2003-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Schwarz-Linek U., Werner JM., Pickford AR., Gurusiddappa S., Kim JH., Pilka ES., Briggs JA., Gough TS., Höök M., Campbell ID., Potts JR. jennifer.potts@bioch.ox.ac.uk Nature 2003 imex curation Only protein-protein interactions imex curation schwarz_linek-2003-1 in vitro In vitro solution nmr solution state nmr solution state nmr solution nmr predetermined predetermined participant predetermined Schwarz-Linek U., Werner JM., Pickford AR., Gurusiddappa S., Kim JH., Pilka ES., Briggs JA., Gough TS., Höök M., Campbell ID., Potts JR. jennifer.potts@bioch.ox.ac.uk Nature 2003 imex curation Only protein-protein interactions imex curation fnba_staa8 Fibronectin-binding protein A fnbA SAOUHSC_02803 protein protein staa8 Staphylococcus aureus (strain NCTC 8325) MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTTVEENGNSATDNKTSETQTTATNVNHIEETQSYNATVTEQPSNATQVTTEEAPKAVQAPQTAQPANIETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVEVAQPRTASESKPRVTRSADVAEAKEASNAKVETGTDVTSKVTVEIGSIEGHNNTNKVEPHAGQRAVLKYKLKFENGLHQGDYFDFTLSNNVNTHGVSTARKVPEIKNGSVVMATGEVLEGGKIRYTFTNDIEDKVDVTAELEINLFIDPKTVQTNGNQTITSTLNEEQTSKELDVKYKDGIGNYYANLNGSIETFNKANNRFSHVAFIKPNNGKTTSVTVTGTLMKGSNQNGNQPKVRIFEYLGNNEDIAKSVYANTTDTSKFKEVTSNMSGNLNLQNNGSYSLNIENLDKTYVVHYDGEYLNGTDEVDFRTQMVGHPEQLYKYYYDRGYTLTWDNGLVLYSNKANGNEKNGPIIQNNKFEYKEDTIKETLTGQYDKNLVTTVEEEYDSSTLDIDYHTAIDGGGGYVDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHHADVVEYEEDTNPGGGQVTTESNLVEFDEESTKGIVTGAVSDHTTVEDTKEYTTESNLIELVDELPEEHGQAQGPVEEITKNNHHISHSGLGTENGHGNYDVIEEIEENSHVDIKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGNQSFEEDTEKDKPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPSYQFGGHNSVDFEEDTLPKVSGQNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKPQSKKSELPETGGEESTNKGMLFGGLFSILGLALLRRNKKNHKA mint q01924_strpy Sfb protein protein strpy Streptococcus pyogenes MNNKMFLNKEAGFLAHTKRKRRFAVTLVGVFFMLLASAGAIGFGQVAYAADEKTVPHRVSQNPEFPWYGYDFYKGPYTRYHNLQLNLNGSKTYQAYCFNLKRFEPKKEGSYFPNWYKRWDGSEETFVKYADNPRKDNESSRVIDVELEKNILRVLYNGYPNNGNGIMEGLEPLNAILVTQNAVWYYSDNSSIFNTDNFFTTEAKDLNIKPEQLSLMRVALKKLIDPKLSEESLKPVPSTFRLNIFESQDKLYQNLLSAEFVPENPPKPGETPEHGPKTPELDGTPIPEGPQRPNESLEPTLPPVMLDGQEVPEVPSESLEPALPPLMPELDGQEVPEVPSESLEPALPPLMPELDGQEVPEKPSVDLPIEDPRYEFNNKDQSPLAGESGETEYITEVYGNQQNPVDIDKKLPNETGFSGNMVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGQTTPQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGQTASQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGQTTPQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGFSETVTIVEDTRPKLVFHFDNNEPKVEENREKPTKNITPILPATGDIENVLAFLGILILSVLPIFSLLKKQTKQ finc_human Fibronectin FN1 FN Cold-insoluble globulin protein protein human Homo sapiens Human MLRGPGPGLLLLAVQCLGTAVPSTGASKSKRQAQQMVQPQSPVAVSQSKPGCYDNGKHYQINQQWERTYLGNALVCTCYGGSRGFNCESKPEAEETCFDKYTGNTYRVGDTYERPKDSMIWDCTCIGAGRGRISCTIANRCHEGGQSYKIGDTWRRPHETGGYMLECVCLGNGKGEWTCKPIAEKCFDHAAGTSYVVGETWEKPYQGWMMVDCTCLGEGSGRITCTSRNRCNDQDTRTSYRIGDTWSKKDNRGNLLQCICTGNGRGEWKCERHTSVQTTSSGSGPFTDVRAAVYQPQPHPQPPPYGHCVTDSGVVYSVGMQWLKTQGNKQMLCTCLGNGVSCQETAVTQTYGGNSNGEPCVLPFTYNGRTFYSCTTEGRQDGHLWCSTTSNYEQDQKYSFCTDHTVLVQTRGGNSNGALCHFPFLYNNHNYTDCTSEGRRDNMKWCGTTQNYDADQKFGFCPMAAHEEICTTNEGVMYRIGDQWDKQHDMGHMMRCTCVGNGRGEWTCIAYSQLRDQCIVDDITYNVNDTFHKRHEEGHMLNCTCFGQGRGRWKCDPVDQCQDSETGTFYQIGDSWEKYVHGVRYQCYCYGRGIGEWHCQPLQTYPSSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIPEVPQLTDLSFVDITDSSIGLRWTPLNSSTIIGYRITVVAAGEGIPIFEDFVDSSVGYYTVTGLEPGIDYDISVITLINGGESAPTTLTQQTAVPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVYEQHESTPLRGRQKTGLDSPTGIDFSDITANSFTVHWIAPRATITGYRIRHHPEHFSGRPREDRVPHSRNSITLTNLTPGTEYVVSIVALNGREESPLLIGQQSTVSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKSTATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRTEIDKPSQMQVTDVQDNSISVKWLPSSSPVTGYRVTTTPKNGPGPTKTKTAGPDQTEMTIEGLQPTVEYVVSVYAQNPSGESQPLVQTAVTNIDRPKGLAFTDVDVDSIKIAWESPQGQVSRYRVTYSSPEDGIHELFPAPDGEEDTAELQGLRPGSEYTVSVVALHDDMESQPLIGTQSTAIPAPTDLKFTQVTPTSLSAQWTPPNVQLTGYRVRVTPKEKTGPMKEINLAPDSSSVVVSGLMVATKYEVSVYALKDTLTSRPAQGVVTTLENVSPPRRARVTDATETTITISWRTKTETITGFQVDAVPANGQTPIQRTIKPDVRSYTITGLQPGTDYKIYLYTLNDNARSSPVVIDASTAIDAPSNLRFLATTPNSLLVSWQPPRARITGYIIKYEKPGSPPREVVPRPRPGVTEATITGLEPGTEYTIYVIALKNNQKSEPLIGRKKTDELPQLVTLPHPNLHGPEILDVPSTVQKTPFVTHPGYDTGNGIQLPGTSGQQPSVGQQMIFEEHGFRRTTPPTTATPIRHRPRPYPPNVGEEIQIGHIPREDVDYHLYPHGPGLNPNASTGQEALSQTTISWAPFQDTSEYIISCHPVGTDEEPLQFRVPGTSTSATLTGLTRGATYNVIVEALKDQQRHKVREEVVTVGNSVNEGLNQPTDDSCFDPYTVSHYAVGDEWERMSESGFKLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE q53971_strdy fnbB protein protein Streptococcus dysgalactiae Streptococcus dysgalactiae MKNKVLKTLVLFLFAVLGIFSIQTVRAAGKSVNLQVKAQTEQINHGDSVRFEVNFGGPGTKVEKNQTVNISFIGDYAKINLPKESLILKDSNDDAILGRVEFSDNKAILIFNEEASHRDDIVGGFFFDARGIYNGDVKSSGKGSIRVEYENKYRIININYNAGGSRTDSVYAKSGVLDDFDSTGRIVNWYFNFNAALNPIKDEDVEFMTTDNLPQTMTWDIEAVKKDPYVVQIRGGYLGTVFSKNGGWIDIEEATKLGIEIIFSGQKLTIKIPKWTNYNGLIPYVKPLNQTSISVKLTAKITEKTMRDQTIEYVENDSIPKIEGIEWNLDKNQFGAKVRIQRSGSWILGVRPGDLKVLKFERDKSKAIPKVKFRLIREDNHEIEAKMGKESYQNLGRFYDFETGEDGSVTLKGLKDGIYLLKEIGNNLVDLSKPIVWDNLSLREKIELNQEQPYKVIISSTQQPNGIVLKVENELKSEKVDIKVTKKWEDYQDKFKKRPDHILLDLEANGEKVEGHTSKIVTKGMDDKWSYTFKDLPKYNDRGEKISYKVVEVQVNEYETPIIEEISPYNFTITNKYINKETTELSGKKIWENDRANQRPEQIQVQLLQNGQKMPNKIQEIKANDWTYKFKDLPKYDETGKEYKYSVEEVTVPDGYKVSYLGNDIYNTRETEFIFENNNFSLEFGNSEVKGQSGSKVITEDTLTSFKGRKLWKNDAATDRPEAIQVQLYADGVAVEGQTKYVSGSGDEWSFEFKNLKKHGESGKEIVYSVKEVTVPAGYDVTYSDNDIINTKREVIIQQGPILGLEETLPTEEHQSGDTTTIEDTRPIDTMSGLSGETGQSGNTTIEEDSTTHVKFSKRDINGKELAGAMIELRNLSGQTIQSWISDGTVKVFYLMPGTYQFVETAAPEGYELAAPITFTIDEKGQIWVDSTLIVGDDPIVMIDNDKPITEASQSIDFEETLPTEQGQSGSTTEVEDTKGPEVIIGGQGEIVDIEENLPTEQGQSGSTTEVEDTKGPEVIIGGQGEVVDIEESLPTEQGQSGSTTEVEDSKPKLSIHFDNEWPKEDKPQLPAVEKPKTKESLPAAGEAEHVLSTIVGAMILFLVSLWGLLKRKASKA fn1-fnbb-4 3473185 N/A 3473187 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3473188 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false Fig 1 fn1-fnbb-5 3473186 N/A 3473188 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false 15N label 15N label 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3473187 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 2 fn1-fnbb-6 3472230 N/A 3473188 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3473187 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 3 fn1-fnba-5 3472230 N/A 3472234 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3473188 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false Fig 3 fn1-fnba-3 3472230 N/A 3472234 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3473188 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false Fig 3 fn1-fnba-4 3472230 N/A 3472234 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3473188 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false Fig 3 fn1-sfb-1 3472230 N/A 3473188 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3472235 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 3 fn1-sfb-3 3472230 N/A 3473188 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3472235 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 3 fn1-sfb-4 3472230 N/A 3473188 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3472235 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 3 fn1-sfb-5 3472230 N/A 3473188 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3472235 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 3 fn1-sfb-6 3472230 N/A 3473188 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris N/A 3472235 unspecified role unspecified role neutral component neutral component purified purified binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false Fig 3 fn1-fnbb-3 3472231 N/A 3473187 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3473188 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed binding region binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false Fig 1, Table 1