IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ kingston-2004-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kingston RL., Hamel DJ., Gay LS., Dahlquist FW., Matthews BW. richard@uoxray.uoregon.edu Proceedings of the National Academy of Sciences of the United States of America 2004 Only protein-protein interactions imex curation imex curation kingston-2004-2 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Kingston RL., Hamel DJ., Gay LS., Dahlquist FW., Matthews BW. richard@uoxray.uoregon.edu Proceedings of the National Academy of Sciences of the United States of America 2004 Only protein-protein interactions imex curation imex curation ncap_measm Nucleoprotein Nucleocapsid protein N NP protein protein MeV Measles virus (strain Edmonston-Moraten vaccine) Subacute sclerose panencephalitis virus MATLLRSLALFKRNKDKPPITSGSGGAIRGIKHIIIVPIPGDSSITTRSRLLDRLVRLIGNPDVSGPKLTGALIGILSLFVESPGQLIQRITDDPDVSIRLLEVVQSDQSQSGLTFASRGTNMEDEADQYFSHDDPISSDQSRFGWFGNKEISDIEVQDPEGFNMILGTILAQIWVLLAKAVTAPDTAADSELRRWIKYTQQRRVVGEFRLERKWLDVVRNRIAEDLSLRRFMVALILDIKRTPGNKPRIAEMICDIDTYIVEAGLASFILTIKFGIETMYPALGLHEFAGELSTLESLMNLYQQMGETAPYMVILENSIQNKFSAGSYPLLWSYAMGVGVELENSMGGLNFGRSYFDPAYFRLGQEMVRRSAGKVSSTLASELGITAEDARLVSEIAMHTTEDKISRAVGPRQAQVSFLHGDQSENELPRLGGKEDRRVKQSRGEARESYRETGPSRASDARAAHLPTGTPLDIDTATESSQDPQDSRRSADALLRLQAMAGISEEQGSDTDTPIVYNDRNLLD q77m42_measm Phosphoprotein P protein protein MeV Measles virus (strain Edmonston-Moraten vaccine) Subacute sclerose panencephalitis virus MAEEQARHVKNGLECIRALKAEPIGSLAIEEAMAAWSEISDNPGQERATCREEKAGSSGLSKPCLSAIGSTEGGAPRIRGQGPGESDDDAETLGIPPRNLQASSTGLQCYYVYDHSGEAVKGIQDADSIMVQSGLDGDSTLSGGDNESENSDVDIGEPDTEGYAITDRGSAPISMGFRASDVETAEGGEIHELLRLQSRGNNFPKLGKTLNVPPPPDPGRASTSGTPIKKGTDARLASFGTEIASLLTGGATQCARKSPSEPSGPGAPAGNVPECVSNAALIQEWTPESGTTISPRSQNNEEGGDYYDDELFSDVQDIKTALAKIHEDNQKIISKLESLLLLKGEVESIKKQINRQNISISTLEGHLSSIMIAIPGLGKDPNDPTADVEINPDLKPIIGRDSGRALAEVLKKPVASRQLQGMTNGRTSSRGQLLKEFQLKPIGKKMSSAVGFVPDTGPASRSVIRSIIKSSRLEEDRKRYLMTLLDDIKGANDLAKFHQMLMKIIMK n-p-3 3583033 N/A 3583035 unspecified role unspecified role neutral component neutral component purified purified N_486-505 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3583036 unspecified role unspecified role neutral component neutral component purified purified P_457-507 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 3583041 3583039 direct interaction direct interaction false false false Fig.5 2A R work (%) = 23.0 R free (%) = 27.5 The two interacting protein chains were linked together with an 8 residue (GS)4 linker. The referenced PDB structure shows the 3d structure of this chimeric protein. The linker is largely disordered in the crystal and hence has no corresponding atomic coordinates. n-p-4 3583034 N/A 3583035 unspecified role unspecified role neutral component neutral component purified purified N_477-505 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 15N radiolabelled 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 N/A 3583036 unspecified role unspecified role neutral component neutral component purified purified P_457-507 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 15N radiolabelled 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 3583047 3583044 direct interaction direct interaction false false false 3583034 3583034 Fig.2-4