DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ koellhoffer-2020-2 in vitro In vitro crosslink cross-linking study crosslink experimental particp experimental participant identification experimental particp Koellhoffer JF et al, Koellhoffer JF., Dai Z., Malashkevich VN., Stenglein MD., Liu Y., Toro R., S Harrison J., Chandran K., DeRisi JL., Almo SC., Lai JR. Glutaraldehyde was the Cross-Linkin agent. jon.lai@einstein.yu.edu Journal of molecular biology 2014 Only protein-protein interactions imex curation imex curation koellhoffer-2020-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Koellhoffer JF et al, Koellhoffer JF., Dai Z., Malashkevich VN., Stenglein MD., Liu Y., Toro R., S Harrison J., Chandran K., DeRisi JL., Almo SC., Lai JR. jon.lai@einstein.yu.edu Journal of molecular biology 2014 Only protein-protein interactions imex curation imex curation koellhoffer-2020-3 ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column experimental particp experimental participant identification experimental particp Koellhoffer JF et al, Koellhoffer JF., Dai Z., Malashkevich VN., Stenglein MD., Liu Y., Toro R., S Harrison J., Chandran K., DeRisi JL., Almo SC., Lai JR. jon.lai@einstein.yu.edu Journal of molecular biology 2014 Only protein-protein interactions imex curation imex curation j7h5l9_9viru GPC protein protein CAS virus CAS virus MHYPWLHSRGRTKCEAHTAKALIIILLTANCKALIAEMLQAAAGNDSSICHGMMFTEPVGSFLGINPNTTDDPFELHALSLNQNVTDKIMIFQNGLKLFGSETRNYSITSGPGLLVGFFGHSRSGVYKNLTFTNNTTFKICVSCKSRFNITLVVYNKFTKKASTTICPDEFVRKKRSDVIVSYNLTCVILHNKNMITHHKTALNAEFSPRGLQQLSTKNDVEPGCDSNVGIFGHSSGTDAGWGIAGFFTLGITNSLQISEIEHVTDAIACKIIKTANYTTNALFLLNKEESEIRDHVVEHELALNYLLAHQGGLCNVVKGPMCCSDIDDFSKNVSDMIDKVHEEMKKFYHEPDPFGGLGSIGFFGTLFGHILQWIPIILLTIFMCFICSWVKK gpc-6 3714958 N/A 3714961 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified J7H5L9:p.[Cys270Ser;Cys324Ser] C270S, C324S mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 254-351 binding region 254-351 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 3.0 direct interaction direct interaction false false false Fig 2a gpc-8 3714958 N/A 3714961 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified J7H5L9:p.[Cys270Ser;Cys324Ser] C270S, C324S mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 254-351 binding region 254-351 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 2a The band intensity shifts from monomer to trimer with increasing reaction time. gpc-1 3714959 N/A 3714961 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified J7H5L9:p.[Cys270Ser;Cys324Ser] C270S, C324S mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 267-351 binding region 267-351 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeast GCN4 coiled coil tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 3.0 direct interaction direct interaction false false false Fig 6,Tab 2 gpc-7 3714960 N/A 3714961 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified J7H5L9:p.[Cys270Ser;Cys324Ser] C270S, C324S mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 267-351 binding region 267-351 binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain false n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false yeast GCN4 coiled coil tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 3.0 direct interaction direct interaction false false false Fig 2b