DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wang-2014-35 in vitro In vitro methyltransferase as methyltransferase assay methyltransferase as weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wang C., Zhu Y., Caceres TB., Liu L., Peng J., Wang J., Chen J., Chen X., Zhang Z., Zuo X., Gong Q., Teng M., Hevel JM., Wu J., Shi Y. DIP original experiment: DIP-260649X Structure (London, England : 1993) 2014 IMEx Structural determinants for the strict monomethylation activity by trypanosoma brucei protein arginine methyltransferase 7. Wang C., Zhu Y., Caceres TB., Liu L., Peng J., Wang J., Chen J., Chen X., Zhang Z., Zuo X., Gong Q., Teng M., Hevel JM., Wu J., Shi Y. Only protein-protein interactions imex curation barquilla-2008-1 9tryp Trypanosoma brucei anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Barquilla A., Crespo JL., Navarro M. DIP original experiment: DIP-133370X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Barquilla A., Crespo JL., Navarro M. Only protein-protein interactions imex curation barquilla-2008-3 9tryp Trypanosoma brucei anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Barquilla A., Crespo JL., Navarro M. DIP original experiment: DIP-133372X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Barquilla A., Crespo JL., Navarro M. Only protein-protein interactions imex curation barquilla-2008-5 9tryp Trypanosoma brucei anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Barquilla A., Crespo JL., Navarro M. DIP original experiment: DIP-133373X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Barquilla A., Crespo JL., Navarro M. Only protein-protein interactions imex curation barquilla-2008-8 9tryp Trypanosoma brucei anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Barquilla A., Crespo JL., Navarro M. DIP original experiment: DIP-133376X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Rapamycin inhibits trypanosome cell growth by preventing TOR complex 2 formation. Barquilla A., Crespo JL., Navarro M. Only protein-protein interactions imex curation o76222_9tryp Non-specific serine/threonine protein kinase PI3K protein protein 9tryp Trypanosoma brucei SVAAVQVIVGIFSLEVLSPGCLVSFYGEVISSIQKQARVQVRKREEILVHLISLVRILKGHLRPHLKEITSTVDSFISVTDLSVLRQVLALLKELCCSLRRRFRPYMSSLLGPIIVLVEENVEETSEIVLDFFSAMGSLLEDHLHTVLPVVCNIIVDTSVPSRCRIVAVKTLICFTKRLPDLCFHASRCVHCLCRVLRESDGGDGVGDEGRLGCSAMEALCTLAGSLGKNFENFVLVVLPAVADRYGETSGEYCRFCHDIYEAIDGKRAPEVSSNGVGKAGGGGAPSLPFTAGTSASPLKDRADAYASLRFHLRKRDQASDEDWNHWLPQLAVNLLRSSSSPSHGRALTLAELHEPFARQMLHSAFAACYADMDEHTQREVIGLLTEVLRGLRVPSEVMQELLNLSEHMERQGIRLSAGGKASIKHSSNFCPFDRQVLMESSANCNLYSKALHYAEIEFLETVREYERSILRGCPKPLPVEDWQNLIKLCEKSIYFCNLLGQRESANGILKFIRQNLPLLTGKKVTELSQMMDAHLFDKLRWWSQSLQAYERRLQQEPNKVSNMVGLLRALDALGEYPRVLEMWRQFSKRVSKREVSKLASMGAHAAWLLRRWDDMEHITSFMSDEDYTGTTALFYKATLAARKKRFREAEKLIDMCRKRVDSKLSALVAESYDRAYDLFVGIQQLSELEELAMATSDPQSAMHWRQLWERRLSVMAYEGWPGTLANHTLLVPPSSEIDMWLRFVSLSRAHGQGSVSTEVLRELLGNQSIESAIENGIPTPAVAMGSFQHLYETNQRDSAIARLQLYVSKVEGSGAQHVSREREDMAVCHAKLAEWLVHQKKAHRTEDELQKIFHHLRRATELDKSNGSIWRTLARVHREAATKPADGSDSSGASGHIMEALSAYLRSVSLSEELEDALGFLSLWFMYGPLLAVQVGSTLKEEIEEVNPTVWLKVIPQIIARISSSNGTVADSVYNLLVLVARRHPQAILYSLNVAHSSYQGKGTADGVEPLKGSHRVLARIAEIHQNGKAMVEDSALVCRELVRCAVLWPELWFNELGRALYQWERQRSAENLLLAMGPLLEQLKRPETMAEAQFAAELRQPLENACCHVERAVSSRHEQFMEEARRIFVSIERRIREQISGMSSLALQLVSPKLHQNGRNLSLVVPGQYREDGNYPLIASFQNVLKVLNSKQRPRRIYINGSNGEIYKFLLKGHEDLRLDERVMQLLGFVNTILEKHSVPKRHDCLIQTYSVTPLSDNAGLVGWVDHCDTLNKIIEDYRVNPRCIRMELDLMRSMCDNLYYLTAIQRVEPFEFPLERTEGVDLVRSFWVKAPSAETWLERRTTYVCSLATMSMVGHILGLGDRHPSNLMIHAFSGRVVHIDFGDCFEVAQQRSIHPEKVPFRLTRMLVKAMEMGGIEGLFRHGCHTVMNVLREEGGSLLALLEAFVHDPLVSWWRDEAEGFSGNGQAASSVQTISVIGSMTNVAEEGSVGSLQLSQLSVQRRATTQQRVNANIEKDTSQTRKPKSVVKRIKRKLRGLEFPQSQEGKSSDGFTVEEQVSRLIEEATSNENLCVHFLGWCPFW q57yz3_tryb2 Tb927.8.3200 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVTTGLSPGALSLLQNAGINLKDFSRAQASYEKLCVELSKGASAAGEGDATAASSMEDLQRLCDAWGEYTVVAQAYFRKVSLAALEQLVSRPRGESSATERAEKLNELCAIGEKWHVGRETMFKLDHPEKLAAVVVEALDSSSCIMRLLALRLARIFAADTEFLRCLFSKGGAGLIGMCFDHENTTELEYVLSLCRRLIELHKASAEPYHELPFGWVYRIAALLDLQDPVPNLLLKRRSQALCVAVMLLRQFPQMATTASLHITLLRYCIEKGAATRDEVQSVLEVILDMFDSPETRQYLGYNDLDVLYAPFLYSTNMCSTEDLTLMNGAKEVLVMLMRNWVGVLWVCSETKGLRAVIDILHLPCDLDRKMVLLTLFNKLLYQLAPHRGITLVEPWRGFKEKQELFPATPNEPADSEGSFVLNTPLGRGHNSSESMISEHVFGDWCDEPDEFVPTTKAIGYHVLDPILGRVLLMLSHHGLPIALVSVIQDPNSSSVLTHAALSLLQDMLTLMDTVLPEKPVKRLHTALNKAVGRLALEGCLSLEGVLSSRIPQRGKTAKGIATTATVAVGNVVGSGWVSALSTASVATYGLGALEQDDADFKRMLKDTNVEGGNSFLSWNYDLLLLLFQGPLKSIRRFRWVLNETLFFRNLIAFYLPVINTSTRTFLTLQPREATPQVCMLGMVMLDLFLSTREGTAALDSFGFSGAMAACLEEVIRGTPYVLNKQRVGKRVGQTLLRMVGRYSMTANGLVVLKDSNMFSTINRMFVELSGERVSAPYPDDTLQEVCLQLLRCMNLGAVPNYGVCEWIRQMFRSALCNESDEIRLCAVKQLRDALWRDFSTSMRWGIETLVQALHDDHSRVVECAFKLLLNVCFCSDEALDYLISLSPTVLMESDTIRENAKQLDLNTLLYCIVGRPSGFRFLQCYGWVEQELRRWEETESANHVTTVENMQSGRALGSGADFREGMSALFRHRHNRTYSDTGLTFQKPITATAGPDSQVTLPSGIFPSHFAAVLCKSNEGCTLFKHSNLWQRSVRRILEQPMLPDIVYECGTFGERDNIDDDDVDMDGTTADDDDANALIEEDPSFVGRWGTEAAKTSTAKQDEKHALRLSREGHLPSSHSVKLLSASRGYQAAGQTYTIECVGDVSELKDAILCVCHAASSDAGFSLMRSVPMLQNQLMALSRFADTASVSSICIVGKCLLARSRRAAEKLTEISHYVVNESNAYMSADGVPYSVAFADNRPSNWTAIGRRDNPSRVSCSPPYFAHMRYDGEMVDGNSKNDEETTDSVGSTPSPVDSRDSSTFCDVIKGLSATSTRILHHIFALSNPVRRDAARQMLSQTLKHQPHIFVNPQMRWLLRHCAQAFRMRYDDRKFIASILEEAPLTKPTPLVRKTVVFVPEPQRG 9671512C9DDE1185 q387i9_tryb2 Raptor_N domain-containing protein Tb11.03.0460 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MKRGTRIITPCRVVFSRLPIQSIMRNRRRVSVTSSTSALVNELLAAFAPHHLPSLSTGFDMGRSSEDIQDIDGHREWYRGGRGRRCPRLLLILCLNNGNDPPDFSRIDPCARLECWEEPYIARMPHIVTGGGSERIAALLQRQYHSQLQGAVSSMAVEVELEDAKKRMANIRLDAREELVVFHYNGHGMPRATGYGEIWMFDKKRTSYVPLSLAEMAEHLSSPTLYVFDCNSAGSLLKFWCEERLHETRPDDMFICACSAGAFLPLNPQLPADVLTSSLTTPLRMASEWYINFSHRKHLLPHVTAEMIRNVPGDISDERTPLGELQLILTAVTDIVAWCIVPSPLYCKLFRRDETTMVLFRNFLLADRLLREVGCVPQTHPPIPEEAHLHHAWDLWDYTLENVICQLPDLLTRDLVLNPNYTYKPSTFFEDQLMSFEVWLKSGDMREQPEELPSVLSALKRPQCCVAALTLLVTYLDSDVIAGKWAILCGILTHITSLLRNQELFLMASVLWMQVLRVDESLGTSELIKRRLGNRFIKVLTLTEKDLCVQTVERGQVAPLRALNNVNRRVGRGDTQASGNNSACPAQISCYLMEGVDLGRCKEIACYLFCQLLRHGGTTACVECWNGGLLNAAASLLESPSAEVRSWSCLVLAQLLFSLQFAKDFVSRKCTTHVGLFTHLLQDKSPIVRGSCVTLLSSLIGFRVSEAPGEHAVRRLQVEKTLLIKLRGMVYDASVIVRQELVLFACTVLFHYGPFLPSLQDTTAKTRYVVYMQEVGQCFPSWFLDEPDVQVKSMLNTSRPTANRCFNSKSVFENAPCAPADLTEPPWTPLAMDDIRSEDLPVLRGMVHDAALMLFSLYQACDKVMVTAALEKLGDNKRPESQRFTNESLRSMSSVVSANSTHFMSEAERARVTRNADNMQRIVLDLHDQKTEPPSMCTTAANRTRSLAVDGHSATYNANHSSGIPGAGDEKVNLDLVYAPHQILQSLLPCDQVICSTLRVLETAMVIATRNQYIYHVSYESYSVQETVHSFQVHLASPLHDILVINDASEQAGLLLVNQRGGYSLIRDCWEKTQGSPVEVAVFSACPPQRTVDIKSAYRSHNANLIYGGPIGYGGGTEIHILSLDEEQVIQQLKVSGDPTITSLSAHTTGRAVFAGCSDGLVRYYDDRQKQGFLGAVGSLRCGTTSYSPMEAVLGAGPVERDVAQLTIAMASRSAVYLYDTRKVNAPYLEVRVNELCDAVVPSSAAKPPPIRAFDAGTHTGMLGVLFADGTYTALNARGRPILRRAIQTRVQGPIFPGSLAVHPLRRVMSVGGEILVVH 12F8BAE200C761C9 q57ut3_tryb2 Serine/threonine-protein kinase TOR Tb927.4.420 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSLGYDARKVEALIRTIAVSSKCDVIYAAEVVKQAKDNQRKNQQEYENLVSCLLFCLENFDVVEGGTAEVKCALVTLATLLQLDLTANQVSQCNSYLSICLTHVQDVSVAFVASRAFGETLVTSMTSDFVQEQVEGAFLAIDLKENITHAQRVCALLMLQEVVTKIPMRILDMLDSLFEKLWGMLASSDAMIREISHLLFMDCGKLLCSRSPVNRKKTVDSLLHQLKFNLAAKSRESNMAGLLAFETVVMTSVGTASQPRYEDMSIMLVPYVMTGGSTNIAELRELLFRCLIVLCRYSTTLFITNQLKDTVGFALKSIENGYQVAASFNMLSEIIVLVTDVAFRPFVNETCNAVRYVSGKSSKPCWEAVRCFSVICHVCPPVDVESYIEPCIENIFRWGLSLPLIESMPHIIASSSAKYRSRLEEGLLDMISVTLCGLPFRQQPGSKGTITMKVESEPTESQITVALDALVQFGFSDSELMGDFLRDSVLPLIDSESVSVRTAAVRTICTLLIPPGVKGELTMARRICVDVILSRMLVVGLSNPDPVVRKGILSSFTEPFYPFLKEVQFVTSIYAALGDEDISCRVAATVLLCRMTFHDPSHILPIFRKQIVQILHTVSTSDNTGAVQSGLQLLDAAASNAPQFVMNFADGIIKALGHHFSTVNIHSSILQHLLRCCTSVAHAAHFFGNTDAIFRVEVDRAYMLLKALPLDSESVELRLWCLRFLSATLGPQIEGRSPYEVYPHLYQQLSGIVKNNDEDLDVRLEALRCAGVIGALDISIFHSTVVNRTCRHEEMKRNEPSTLSHAMCCRVVIRSIASLLDLSEKRFSGGKDNVLRVGIQTILNIAESCPDSRGEIAVVIPPVVRAVSELSACRLLGTLLHELTQIIRYVGVAALSHAHLLYNLIDESWVKSSKYRFLAVRLALTLVTLEADGKAESRRSNMRMSPIKFDALNDIESSESLRYAIIEYILKHTQTLKTCVETVVTNLLRAAQRCPIDFALSVITTLRVVCFRLHVDEFAGLIVRGLLSCLKTRLSTSMRATEQNAFVSRIMGVFCVLMTVLQSEFVKYSSEVLRTLKTLRVSNNELITLHGTLMKGGVCTLSKGFVTEHQKQVVRLLKECEGIALRHVSDLDKVLSTRGNVDEKGWNSDDGERPLPFSEKLIISTTRTAPLTKEEWLRWLNDFSCVIMQESPYRVFRCISLPLTANTSPLVDCSPQFTQDIAPHAFRALWNFASASVRSSLADFFFQVLQQAMESSAVPDEVVSALLTVAEYMDNVGMALPIAPSILSRCAWGRGMLAKALYWCEAAYRKDPRGTAKYLFSLYRELQQSGLLMVTGHSESLKLLQGCIDNERSESDGSDEYSVKWLSRQESLRRMCGVGGRSRSDANSSFNGEDVKLDPEMENEVNTLYALSEAGDYDAVLEQWKVLFQKYNGRDEQKREENTLFYVSQCAADASIRLQAWDTLEDTTRWQLNDTVSYHVSRAALSIHKRRYEEATSAINEGRKLLLEDLSGLLHQSYTRAYEGLVIAQKLSELEEIVTVKRAEVVSSTHHLSRVLRLWSQRIEMMSSTVSAWKEVLGVRGLLIPPGEDVATRLRFVNLCRREKAKQHERFILCQLLGYRCPTSEQLMHSNANPRVVMQYISFLSANGELGPKSSYGLERDLLKRMIDTHSRNENAFLLSSAYARLGSSAELSEAVQCYKAATTYCPKWFHAWRMFAESNAEMLDVTFSDATCAAAIEGYIQSIKLGNSDSTIIQDVLKLLTLLSRHCDQESGLKELRKRVFEVSPRAWKLVVPQLIARLDSGSDGSCELVAEILTTVSFDCPLSLIYPLNLCAMSNSRRRRKYANIILDKLQSKHPLVVNQGRILVDELVRTSDLLHEQWYHMLECSATAFFDRNDYKGMLDVLLPLHEKLHLSTDTIVEAEFTCKFGNKLDRAQEWLQSYLRTGSIADLHSAWNIYHAVYQQIDKQIRSQSKLHLSFCSPKLFEARNLAVGLPDAMPSEDCAVRCTASFNGTLTVIGSKQRPKRLSVTTVDGETQKYLLKGREDLRLDERVMQLFRLVNTLMMTDSRTCKNPGFQIQRYSVTPLKDTVGLIGWVSGCDTLHTLVTKYRKFRGIPPELELRMMNQIIVCDRPKTYDHLTMISKVEVMEFLADHTSGHDVRKAMWASASNCEMWLEQRQVFTTSSATTCMVGYILGLGDRHPNNVMIQRTSGLFVHIDFGDCFEVAMTRQMFPERVPFRLTRMIRNALDVFGVDGPFRSIAETAMCVLRDGSRSVLAILEAFIQDPLISWRLLNHMKKEPGDSTVHKNIDEQSEAVGPRMSSVAFPGAPVRDDMFTVDSKLTRCASIPDDDVVHKGVKVFQRVRSKLKGEEFLRTKGDTKSTDPKVQVARLIVEATDITNVAQSWAGWYPFW A3BCB24DC0FC8495 q9xy40_9tryp RBP16 protein protein 9tryp Trypanosoma brucei MIRASIVKRMAPCTLFLNKGKVISWMSGRGFGFIEDDADKKQHFVHFSALQTETGGFRALTVGQEVEFEVASQDGRTRAENVTSPGGAKLPSGPRPPEGAGRGRGFGGGRGGRDFGGDRNSGRGRNDNQQGGQHQSFSDDF anm7_tryb2 Protein arginine N-methyltransferase 7 PRMT7 Tb927.7.5490 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MPPKQHRHQKKDKNDNALQNTIGFVPPGATLASVSGYRPPDAFVNRIDRNIPVPARLRHTPVSLIEAVNDFHYAMMNDEERNNFYYEVLKKHVTPETGVLEIGAGSGLLSLMAAKLGAKWVVAVEGSEELAKLARENIRANNMEHQVKVLHMMSTELKSKHLPEPPDVLLSEIFGTMMLGESALDYVVDVRNRLLKPTTKIIPQFGTQYAVPIECDALHRISSVSGWRDLDLKHMMTLQDTVSIVFAKHYGIRMNSVNFRRLSDPIELFRVDFSSSNRNDIPRRKHFDVVAKESGTAHAMLFYWKVTDDEFVMSTDPEDTVNNFPRDMQWGQALQLLDASNGPLPTPVVFTEGKNYNFECNFSGDRVILHMQLCPESGNGEMTECEGKTT 32C2C7EEF1EB45B1 rbp16-tbprmt7-1 3264820 N/A 3264829 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3264830 enzyme enzyme unspecified role unspecified role Region 36-378 Region 36-378 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli methylation methylation reaction methylation false false false pi3k-tbavo3-1 3177660 N/A 3177668 unspecified role unspecified role bait bait 9tryp Trypanosoma brucei N/A 3177669 unspecified role unspecified role prey prey myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 9tryp Trypanosoma brucei N/A 3177670 unspecified role unspecified role prey prey myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 9tryp Trypanosoma brucei association association false false false tbraptor-pi3k-1 3177662 N/A 3177668 unspecified role unspecified role prey prey 9tryp Trypanosoma brucei N/A 3177670 unspecified role unspecified role bait bait 9tryp Trypanosoma brucei physical association physical association false false false tbavo3-pi3k-1 3177664 N/A 3177668 unspecified role unspecified role prey prey 9tryp Trypanosoma brucei N/A 3177672 unspecified role unspecified role prey prey 9tryp Trypanosoma brucei N/A 3177669 unspecified role unspecified role bait bait myc tag myc tag experimental feature experimental feature predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 9tryp Trypanosoma brucei association association false false false tbraptor-pi3k-2 3177667 N/A 3177668 unspecified role unspecified role prey prey 9tryp Trypanosoma brucei N/A 3177670 unspecified role unspecified role bait bait myc tag myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 9tryp Trypanosoma brucei physical association physical association false false false IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ schumacher-2006-1 Crystal structures of T. brucei MRP1/MRP2 guide-RNA binding complex reveal RNA matchmaking mechanism. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Schumacher MA., Karamooz E., Zikova A., Trantirek L., Lukes J. maschuma@mdanderson.org Cell (0092-8674) 2006 Only protein-protein interactions 2 imex curation schumacher-2006-2 Crystal structures of T. brucei MRP1/MRP2 guide-RNA binding complex reveal RNA matchmaking mechanism. in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Schumacher MA., Karamooz E., Zikova A., Trantirek L., Lukes J. maschuma@mdanderson.org Cell (0092-8674) 2006 Only protein-protein interactions 2 imex curation estevez-2003-2 The roles of intersubunit interactions in exosome stability. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Estevez AM., Lehner B., Sanderson CM., Ruppert T., Clayton C. aestevez@ipb.csic.es J. Biol. Chem. (0021-9258) 2003 estevez-2003-1 The roles of intersubunit interactions in exosome stability. 9tryp Trypanosoma brucei tap tandem affinity purification TAP TAP tap fingerprinting peptide massfingerprinting fingerprinting Estevez AM., Lehner B., Sanderson CM., Ruppert T., Clayton C. aestevez@ipb.csic.es J. Biol. Chem. (0021-9258) 2003 whitby-2000-1 Structural basis for the activation of 20S proteasomes by 11S regulators. in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Whitby FG., Masters EI., Kramer L., Knowlton JR., Yao Y., Wang CC., Hill CP. PDBe - Data obtained from the Protein Data Bank Europe Nature (0028-0836) 2000 schimanski-2005-1 Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination. 9tryp Trypanosoma brucei affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Schimanski B., Nguyen TN., Gunzl A. In some cases the tag used was a derivative of the original TAP tag where the calcium binding peptide was replaced by the 12 amino acid epitope protein C (ProtC) for a ProC-TEV-ProtA tag. This tag is referred to as PTP in the text. An anti-ProtC matrix replaced the calmodulin affinity resin for purification of proteins. Figure 2(B). Figure 3 (B) and (D). gunzl@uchc.edu Eukaryotic Cell (1535-9778) 2005 Only protein-protein interactions imex curation yang-2009a-2 RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma brucei. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yang X., Figueiredo LM., Espinal A., Okubo E., Li B. b.li37@csuohio.edu A GAD cDNA library generated with T. brucei 427 cDNA Cell (0092-8674) 2009 Only protein-protein interactions 6 imex curation yang-2009a-1 RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma brucei. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yang X., Figueiredo LM., Espinal A., Okubo E., Li B. b.li37@csuohio.edu A GAD cDNA library generated with T. brucei 427 cDNA Cell (0092-8674) 2009 Only protein-protein interactions 6 imex curation yang-2009a-3 RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma brucei. 9tryp Trypanosoma brucei anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yang X., Figueiredo LM., Espinal A., Okubo E., Li B. b.li37@csuohio.edu A GAD cDNA library generated with T. brucei 427 cDNA Cell (0092-8674) 2009 Only protein-protein interactions 6 imex curation yang-2009a-4 RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma brucei. 9tryp Trypanosoma brucei anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Yang X., Figueiredo LM., Espinal A., Okubo E., Li B. b.li37@csuohio.edu A GAD cDNA library generated with T. brucei 427 cDNA Cell (0092-8674) 2009 Only protein-protein interactions 6 imex curation yang-2009a-5 RAP1 is essential for silencing telomeric variant surface glycoprotein genes in Trypanosoma brucei. 9tryp Trypanosoma brucei fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Yang X., Figueiredo LM., Espinal A., Okubo E., Li B. b.li37@csuohio.edu A GAD cDNA library generated with T. brucei 427 cDNA Cell (0092-8674) 2009 Only protein-protein interactions 6 imex curation q387v6_tryb2 Tb10.61.0780 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MNGRYARQSGCFRGKRPREITTQKLFNRAIEHFQSIYNDSVERHERETFASRAVFEKQCGVDREHFNRAHGSLAKHGKGKDFALVLEQFENEEAKLQREHEEELQRVYPLAFLELDTAAVLSLTLIPRLKSKLDLLERQLTQRRKFTNQHQGDAAARGSHSRDVDPPVDAKESDDKNGADGVPSELNDAKTGIEEDDVGTLCVGRGAYITGDEPSEVEESDSSALVQVLDTSVEDGKVGTGTGGGFRNHVHRLVSRGLGNILSIPTDVMRAGAADFARIGLLLNTESRRQRRDERRKFLRSHMEVCGPEGKTIGKWELKRLLQEGKEDKLKQEEIFAALEAAKTMPEFTGSDMNANIVESMENDGAPRKTAGASTASRETGDTTASETVVKRHTKIVLRGNGSRRDDGVPFTRKDFEWTSLGLDDESIGEGSRMEKLAESYMWPKRTEDRIPHILCGEVIPTYFRVRQYQLDTSSPTTATVQMSATGEDGCPSTLLDDLAEATQLDPVREGMSNGEKGVVTKKSPLVFRIPFRAKSVWLKVDTLGDFIASQVKFFGAVTSREI q38bb7_tryb2 Tb10.70.2360 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MYRESLLGVALGATLAELESDCLLTEGQKQQLWEIFDIAMDRTLAEAPVRSQVRVRTSAPSLVGHATLGGESGLSSIDAPTLSTRPAEMEFPLPPSEVTHTVATTPCDVLDEEVLYPVYRVKDGMWTILLKDPILELTDATGSTETIRLDYLKVYLKEVVSRSATSVSASSRKKRKRSRP q4gyy6_tryb2 Tubulin beta chain TB927.1.2330 TB927.1.2350 TB927.1.2370 TB927.1.2390 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MREIVCVQAGQCGNQIGSKFWEVISDEHGVDPTGTYQGDSDLQLERINVYFDEATGGRYVPRSVLIDLEPGTMDSVRAGPYGQIFRPDNFIFGQSGAGNNWAKGHYTEGAELIDSVLDVCCKEAESCDCLQGFQICHSLGGGTGSGMGTLLISKLREQYPDRIMMTFSIIPSPKVSDTVVEPYNTTLSVHQLVENSDESMCIDNEALYDICFRTLKLTTPTFGDLNHLVSAVVSGVTCCLRFPGQLNSDLRKLAVNLVPFPRLHFFMMGFAPLTSRGSQQYRGLSVPELTQQMFDAKNMMQAADPRHGRYLTASALFRGRMSTKEVDEQMLNVQNKNSSYFIEWIPNNIKSSVCDIPPKGLKMAVTFIGNNTCIQEMFRRVGEQFTLMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATIEEEGEFDEEEQY q4gyy5_tryb2 Tubulin alpha chain TB927.1.2340 TB927.1.2360 TB927.1.2380 TB927.1.2400 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MREAICIHIGQAGCQVGNACWELFCLEHGIQPDGAMPSDKTIGVEDDAFNTFFSETGAGKHVPRAVFLDLEPTVVDEVRTGTYRQLFHPEQLISGKEDAANNYARGHYTIGKEIVDLCLDRIRKLADNCTGLQGFLVYHAVGGGTGSGLGALLLERLSVDYGKKSKLGYTVYPSPQVSTAVVEPYNSVLSTHSLLEHTDVAAMLDNEAIYDLTRRNLDIERPTYTNLNRLIGQVVSSLTASLRFDGALNVDLTEFQTNLVPYPRIHFVLTSYAPVISAEKAYHEQLSVSEISNAVFEPASMMTKCDPRHGKYMACCLMYRGDVVPKDVNAAVATIKTKRTIQFVDWSPTGFKCGINYQPPTVVPGGDLAKVQRAVCMIANSTAIAEVFARIDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAALEKDYEEVGAESADMDGEEDVEEY q387i8_tryb2 DNA-directed RNA polymerase subunit beta Tb11.03.0450 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSTKNSVVGSASGSHASTAGETVTSTRSGTSSRHSRQRRQQYDYVKNPSEPMTAMERLKLVRCSIARLRYLGERHRHTSKLDTTGGDRLLSFFEGLRKRVLKLEYNPRVVAKSKVGAPKHLDYIRGHANALLGEASTIISECEQAMDRSEAVYEGLAYHQLQDTHRELENLVGLLSGAPSDMESAGSLKLLADLVSPHVAEFDAFAEQRVPEFLQELKNEGFLFSSVINDSQQPANAIGLLSVFIVDVAIGVRPGRSARLVIQHALHNLARLKSRVDKADLTLGAEEDSSNNEHHRMQSLEFEVIRGRLREAKEELERIRDKWDHDEGDIFSRAGTGVQGLQSIIDAKRVKESTVIEEAQAIHDRTVYACLPFASPQYCRIRSETYSQPCHVRVGFKFVAKGDGSVSRTKTQLIYNLMPEFLILMRQQGIAPSDQERFATLAPSKYELWLSLGELPEMVLGARCALRGVNQRLDHFRALEEQKEVGGYFVLRGGERILRNLILQRCNVPLNIERERFATVDSFFSPRAVIIRCKRPSGLTVQNYFYYATKGEVIFTFARRVVWNIPALLVLSALNTRHRTSLDIYKLLTIGLTNGRSPHAARVEALLQHHSQMPYSTLDNFLDYLAVVGLMYRRYHESSFIFRFLPQYHTSFATQHDAWYGLFMLRRHMLPHLNTAEATPDLPPTATSREIREWLSTGLQEELAAKFDALIGILRQLLVFNDGATGNQGNDVLAYQEVFTVSQVLIGAFEVSLMKTMGAVTFRMAGQLSPEIFQATMCLAETPIREAANTLEKLNQLLEYSCRNNGPDPLITLNRLLLTGNFALDREEDFYSPQTAGWAVMTEHLNFYRSFEQLMCVHRGKSIAEMRSSDVRRYPCEAYGFICMVHSPDGEDCGVLNHLSISTVLSASPRVGSSQYKILLAFIEEELKNVRNTTSLISCVDQLVGTVPVWLEGRLLGYLSPNEALSASKALTQKKALRSRGVVGVTGIVRRDSISSLNTMEVVYIPPGSKDPMGLYIFYQYGRLMRPVLQIESPSRGGDLPFPVVYLGTWEQSWRDIAAVPSDLFDGFDQLRRKYEFMEQNGFNILSFTSSTIPFFEHNCSPRNLFQCGLSKQTAGTQLQTQAWRRDAKLFRMYYPQRYISRTLPMDYFGLDDVSLGVNAVVAILAYTGYDLDDAMILNSTAKQFGMLNAGVTVAKIVKASGKGDSNDVFVFHNCLPDGQPFTPELDRHGLPKKRAVPNATAYFSFDTDHKYPSLRDSSAVYCCAKRFEYIDPLTNEKTYEYTRHQITKWRHFDKGEDAWVHSVIPLTYDGPDPTSVLVLFRIPRPPVIGDKFSSRHGQKGTLPLDIRSHDLPFSTRDGITPDIIINPHAFPSRMTVGMVLEMMGAKLGCIQGRLCDHSAWSVIDEQPRSAKTIGDALEALGYNRYGREKLIDGITGEEIEAEVLVGISGYQRLRHMVSDKWQARARTDSHTHRAVTKTGQPVKGRKRHGGVRVGEMERDALLSHGVAEVVIDRLLHVSDKTKAFICVECGSMLSIYERHATEYSTWKTCKFCSAGLHETDDTIAFVEIPQVLRLWATELTSVGIRVVLKTSETSL q383n7_tryb2 Tb11.01.2190 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSAPMARRDYFSMGSRCCIACGQWIPSEHDMPRSGSAAAGGLQCRRCNVVQPERPMPDFEKASEVTTAALLFTKAEIDAAWKSVKHSLEEARREDVGVAQTDNRVEEEAFCEKCGVPRKCKVFARQIRSADEGQTVFYQCTSCDSEWQLNS q57u75_tryb2 Tb927.5.3910 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MRRNNRTRCTIDDVNGMLARNAQLRNALQERYKQLKMRYEQLAALRAALSPSRGVTLRKLGVRQETADGAEEVRFLDDYTTGGVGNPPFRDAGIYSAKNIVCYAPVPPTRDELRWKGVTLAFPQLAYVHSLAMLPETANSFSKALQWSREEDSALREQVHAYKGARCGPSFWKALGAPGQSRFEVASHYIRLQQLGLIEPGKNDKTMMWIPEEQRDIALREAVWRHLGDEGGIMAAYVEIISVAARKCVYLSEVTCNESLVFPPYVWVKRTTFNWLTSLLIQKAKAPLVRCEGEFSDDTPLCMHFKSDISLKLSAEDMMACLLAFKGEVFGEVGGLRFIERAFLPKNRTYALKATDFMAKREGKKHKLDEEV q4krh6_9tryp protein protein 9tryp Trypanosoma brucei MQQRLVLIATDFVTLYQEALSRQLLTPAALTPDAFKDLFDRINVEYMHYAGAGATQPYFEDVVENLLQLAAAYITLPPDAAPNSRAFGVYLTFFLYATQPAIETSPVKVQISLGTLQRYVEDIDSTARDNQGVITSLGCRVSDGEKRLLLALHKSGALKVMPFIDDSLYVRTLIEVHEQAGLPLLTCVAPQRSNPSPHIALEGGTCVDDDLSNQLHAYREMRRRINTESLLKRK q38e05_tryb2 Tb09.211.1510 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MPPKQLVDPFSLQVGNKKETTLANVAAHTAVTLTDIRRRNPQLDVYKISDKLPAGTIVHLPQTLSERGNPYGCFFTIGEAEDAMRKLEARLDRLLEEEGPNLPELPTRRVLSKMERIVTQVKEEGERQLARVREEYSECERRPALLQFLVDLEPVKRLREQLDEEYDGRKLRLKVMDEGNSELFRQAEPRYPNKGIAASLRHNVIDTSEDFCLTEHSHRWEFTFFTPGSTTEPQETWAVLSCQSLGTLLDTFVCRNCIPVNISKNAFFFIGGTFYVDNRHAGEGGEDYEDLTAPIRHFDPCGEGASTEGETRQKNIAFGNCPVKYVSQTTFGDLNLRLGEYGVMRHLGWCNHYFYLSSVTSLRGFDRDDHTRAAYPQRVMKTPTRVVRCRLCRSHPATVVCYNDEISPESPCPYCVPCFELLHATDEGEVEEGKFFAIRLPQGKYYTA q387r9_tryb2 TATA box-binding protein-like 1 TBP-like factor Tb10.61.0330 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MDNDFTGNFDMDENDFFIDEDEGDMDFLQRAAGASGVYVDAAPKVVMENNEQMKGECSDRVAEVNIQELFPNADPATVPLVVGIIAQAKLGVGVDLRALSCATRNVEFTPRVRCPAATLRLHEPNAVVQVRTSGLLGIIGSTSVGEARQATELAARIIRKALNLNFSSVQFRVRSLMARFDLCHPIRLDELAKHEGVFCSYEPDRFSGCIVRLSGSSRGNQWQVCCTVFVTGKVIVLGARSREELLDAFYTVLPIIAQYAKR q388r6_tryb2 Tb10.389.0120 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MDSVKSFKMIRLYRAFNTVIQMAIDRGFVVSHPSDVANAIRDSRLYDITDGLNYQWFTQRCSSYLQRHRQTTAEEEEEAKQEGRPGKDPSKSKRTKALRDASQDFLYSSLELVCKGSSSRIGFGGGAISCKTEDEVDGRGPTAEPSSRKKNETILCAFFVQSLGVDVLNNLREVAQRRNASSMIVVVGSKVRGGRRSIREAGGGLHPGTTGQVIRIQVFEEDELAYNISRHQSVPKHVPMTDVEVRAFLEQRKLMITQLPRILNDDPMVEYLDLPRGSIIRIERRTDEGSTYEMYRHVI q387x6_tryb2 Plastid-encoded RNA polymerase subunit alpha Tb10.61.1040 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MVKVYNLQRRTETLPTVRRTAPNAEELEYQSHLSRPAGAGDGEPTRRVETITFDMDNVSPPVANMFRRLMLTEVPVFAFDRILIEHNDGVVPDELLAHRIGLVPLAGPVSSMQYITDSASVGFDNLDPERVLLFELDVTAKRDVPTTSVYSGHLKWQPIAKQEELAARSEDDRVFLVHPDIVLTRLGPGQRLKLRAIAIKGIGAVHAKWTPVAACFYEMKQAKAGAEDSDGRASGRGRSRSGRGRVDANEEESNATRPRSASGNHSHEPHRETVNEVIAKRDTSSVRFTVESIGQLYAVDVFRQALILFAERIRDLAVRIRSTEPRMTGAAATAFTE q57ul1_tryb2 Tb927.8.5390 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MSTVNRLQCIGKLGEGTYGVVYKARELATGQIVAYKRIIVGSEDDGAPSTAIREIALLKVLRHNNIVRLYDVLFEPPKLTLIFEYCEYDLRRFMHKHDSRVRTQVKEILKQVLLGLRYMHQRSVVHRDLKPDNIFVRVDGISGSEGSEMSVSSRETEVVNTRGHWDDIDEAANCPKNANPTENPNARIASEDHGADGTVPSGSTTPTSQLVIKLGDYGMARIESIPVKKYSHDVASLWYRSPDALLGSAMYGFAVDLWSVGCIFAEMVTGAPLIRGNTDVDQLLKTFKLLGTPTPETWPSMKNCPKAVQLLKAAAELARGEPAENGKKTKNSRGVHKQHERNLPNVQKTATGTSHLSAVMRVGIDGESGCVADPRDELYHFPPELCFPSAFDEYVKASGFQGRVGVEGTDFLRRLIRYEPSQRMSVQEALSHPFVSNTVALMQRPLDVMASMLRQSLREHSVYP psa4_yeast Proteasome subunit alpha type-4 PRE6 YOL038W Proteasome component PRE6 Macropain subunit PRE6 Proteinase YSCE subunit PRE6 Multicatalytic endopeptidase complex subunit PRE6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGYDRALSIFSPDGHIFQVEYALEAVKRGTCAVGVKGKNCVVLGCERRSTLKLQDTRITPSKVSKIDSHVVLSFSGLNADSRILIEKARVEAQSHRLTLEDPVTVEYLTRYVAGVQQRYTQSGGVRPFGVSTLIAGFDPRDDEPKLYQTEPSGIYSSWSAQTIGRNSKTVREFLEKNYDRKEPPATVEECVKLTVRSLLEVVQTGAKNIEITVVKPDSDIVALSSEEINQYVTQIEQEKQEQQEQDKKKKSNH 73AEE63B836E618A psa2_yeast Proteasome subunit alpha type-2 PRE8 PRS4 YML092C Proteasome component Y7 Macropain subunit Y7 Proteinase YSCE subunit 7 Multicatalytic endopeptidase complex subunit Y7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTDRYSFSLTTFSPSGKLGQIDYALTAVKQGVTSLGIKATNGVVIATEKKSSSPLAMSETLSKVSLLTPDIGAVYSGMGPDYRVLVDKSRKVAHTSYKRIYGEYPPTKLLVSEVAKIMQEATQSGGVRPFGVSLLIAGHDEFNGFSLYQVDPSGSYFPWKATAIGKGSVAAKTFLEKRWNDELELEDAIHIALLTLKESVEGEFNGDTIELAIIGDENPDLLGYTGIPTDKGPRFRKLTSQEINDRLEAL 12D7DFB1AA10E9FE psa3_yeast Proteasome subunit alpha type-3 PRE9 YGR135W PRS5 Proteasome component Y13 Macropain subunit Y13 Proteinase YSCE subunit 13 Multicatalytic endopeptidase complex subunit Y13 protein protein yeast Saccharomyces cerevisiae Baker's yeast MGSRRYDSRTTIFSPEGRLYQVEYALESISHAGTAIGIMASDGIVLAAERKVTSTLLEQDTSTEKLYKLNDKIAVAVAGLTADAEILINTARIHAQNYLKTYNEDIPVEILVRRLSDIKQGYTQHGGLRPFGVSFIYAGYDDRYGYQLYTSNPSGNYTGWKAISVGANTSAAQTLLQMDYKDDMKVDDAIELALKTLSKTTDSSALTYDRLEFATIRKGANDGEVYQKIFKPQEIKDILVKTGITKKDEDEEADEDMK B43BB05233A01A9E psa1_yeast Proteasome subunit alpha type-1 SCL1 PRC2 PRS2 YGL011C Proteasome component C7-alpha Macropain subunit C7-alpha Proteinase YSCE subunit 7 Multicatalytic endopeptidase complex C7 Proteasome component Y8 SCL1 suppressor protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGAAAASAAGYDRHITIFSPEGRLYQVEYAFKATNQTNINSLAVRGKDCTVVISQKKVPDKLLDPTTVSYIFCISRTIGMVVNGPIPDARNAALRAKAEAAEFRYKYGYDMPCDVLAKRMANLSQIYTQRAYMRPLGVILTFVSVDEELGPSIYKTDPAGYYVGYKATATGPKQQEITTNLENHFKKSKIDHINEESWEKVVEFAITHMIDALGTEFSKNDLEVGVATKDKFFTLSAENIEERLVAIAEQD 5B938C862BC8D7EB psb3_yeast Proteasome subunit beta type-3 PUP3 Proteasome component PUP3 YER094C Macropain subunit PUP3 Multicatalytic endopeptidase complex subunit PUP3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDPSSINGGIVVAMTGKDCVAIACDLRLGSQSLGVSNKFEKIFHYGHVFLGITGLATDVTTLNEMFRYKTNLYKLKEERAIEPETFTQLVSSSLYERRFGPYFVGPVVAGINSKSGKPFIAGFDLIGCIDEAKDFIVSGTASDQLFGMCESLYEPNLEPEDLFETISQALLNAADRDALSGWGAVVYIIKKDEVVKRYLKMRQD 5AA92D746454BCB7 q9u8g2_9tryp protein protein 9tryp Trypanosoma brucei MPPKRAALIQNLRDSYTETSSFAVIEEWAAGTLQEIEGIAKAAAEAHGTIRNSTYGRAQAEKSPEQLLGVLQRYQDLCHNVYCQAETIRTVIAIRIPEHKEEDNLGVAVQHAVLKIIDELEIKTLGSGEKSGSGGAPTPIGMYALREYLSARSTVEDKLLGSVDAESGKTKGGSQSPSLLLELRQIDADFMLKVELATTHLSTMVRAVINAYLLNWKKLIQPRTGSDHMVS psb4_yeast Proteasome subunit beta type-4 PRE1 YER012W Proteasome component C11 Macropain subunit C11 Proteinase YSCE subunit 11 Multicatalytic endopeptidase complex subunit C11 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDIILGIRVQDSVILASSKAVTRGISVLKDSDDKTRQLSPHTLMSFAGEAGDTVQFAEYIQANIQLYSIREDYELSPQAVSSFVRQELAKSIRSRRPYQVNVLIGGYDKKKNKPELYQIDYLGTKVELPYGAHGYSGFYTFSLLDHHYRPDMTTEEGLDLLKLCVQELEKRMPMDFKGVIVKIVDKDGIRQVDDFQAQ ED43D319A5895B40 psb6_yeast Proteasome subunit beta type-6 PRE7 PRS3 PTS1 YBL0407 YBL041W Proteasome component C5 Multicatalytic endopeptidase complex subunit C5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MATIASEYSSEASNTPIEHQFNPYGDNGGTILGIAGEDFAVLAGDTRNITDYSINSRYEPKVFDCGDNIVMSANGFAADGDALVKRFKNSVKWYHFDHNDKKLSINSAARNIQHLLYGKRFFPYYVHTIIAGLDEDGKGAVYSFDPVGSYEREQCRAGGAAASLIMPFLDNQVNFKNQYEPGTNGKVKKPLKYLSVEEVIKLVRDSFTSATERHIQVGDGLEILIVTKDGVRKEFYELKRD 770A4DF1DF0A0F0E psa7_yeast Probable proteasome subunit alpha type-7 PRE10 PRC1 PRS1 O6650 YOR362C Proteasome component C1 Macropain subunit C1 Proteinase YSCE subunit 1 Multicatalytic endopeptidase complex subunit C1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTSIGTGYDLSNSVFSPDGRNFQVEYAVKAVENGTTSIGIKCNDGVVFAVEKLITSKLLVPQKNVKIQVVDRHIGCVYSGLIPDGRHLVNRGREEAASFKKLYKTPIPIPAFADRLGQYVQAHTLYNSVRPFGVSTIFGGVDKNGAHLYMLEPSGSYWGYKGAATGKGRQSAKAELEKLVDHHPEGLSAREAVKQAAKIIYLAHEDNKEKDFELEISWCSLSETNGLHKFVKGDLLQEAIDFAQKEINGDDDEDEDDSDNVMSSDDENAPVATNANATTDQEGDIHLE 1E51D904CF336747 psa6_yeast Proteasome subunit alpha type-6 PRE5 YMR314W YM9924.06 Proteasome component PRE5 Macropain subunit PRE5 Proteinase YSCE subunit PRE5 Multicatalytic endopeptidase complex subunit PRE5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFRNNYDGDTVTFSPTGRLFQVEYALEAIKQGSVTVGLRSNTHAVLVALKRNADELSSYQKKIIKCDEHMGLSLAGLAPDARVLSNYLRQQCNYSSLVFNRKLAVERAGHLLCDKAQKNTQSYGGRPYGVGLLIIGYDKSGAHLLEFQPSGNVTELYGTAIGARSQGAKTYLERTLDTFIKIDGNPDELIKAGVEAISQSLRDESLTVDNLSIAIVGKDTPFTIYDGEAVAKYI C43CBCC1C6B9E39E psb1_yeast Proteasome subunit beta type-1 PRE3 YJL001W J1407 Macropain subunit PRE3 Proteinase YSCE subunit PRE3 Multicatalytic endopeptidase complex subunit PRE3 Proteasome component PRE3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNGIQVDINRLKKGEVSLGTSIMAVTFKDGVILGADSRTTTGAYIANRVTDKLTRVHDKIWCCRSGSAADTQAIADIVQYHLELYTSQYGTPSTETAASVFKELCYENKDNLTAGIIVAGYDDKNKGEVYTIPLGGSVHKLPYAIAGSGSTFIYGYCDKNFRENMSKEETVDFIKHSLSQAIKWDGSSGGVIRMVVLTAAGVERLIFYPDEYEQL BD1FCE649678D86B psb2_yeast Proteasome subunit beta type-2 PUP1 YOR157C Macropain subunit PUP1 Proteinase YSCE subunit PUP1 Multicatalytic endopeptidase complex subunit PUP1 Proteasome component PUP1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAGLSFDNYQRNNFLAENSHTQPKATSTGTTIVGVKFNNGVVIAADTRSTQGPIVADKNCAKLHRISPKIWCAGAGTAADTEAVTQLIGSNIELHSLYTSREPRVVSALQMLKQHLFKYQGHIGAYLIVAGVDPTGSHLFSIHAHGSTDVGYYLSLGSGSLAAMAVLESHWKQDLTKEEAIKLASDAIQAGIWNDLGSGSNVDVCVMEIGKDAEYLRNYLTPNVREEKQKSYKFPRGTTAVLKESIVNICDIQEEQVDITA 13A8F2B75584539A psb7_yeast Proteasome subunit beta type-7 PRE4 YFR050C Macropain subunit PRE4 Proteinase YSCE subunit PRE4 Multicatalytic endopeptidase complex subunit PRE4 Proteasome component PRE4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNHDPFSWGRPADSTYGAYNTQIANAGASPMVNTQQPIVTGTSVISMKYDNGVIIAADNLGSYGSLLRFNGVERLIPVGDNTVVGISGDISDMQHIERLLKDLVTENAYDNPLADAEEALEPSYIFEYLATVMYQRRSKMNPLWNAIIVAGVQSNGDQFLRYVNLLGVTYSSPTLATGFGAHMANPLLRKVVDRESDIPKTTVQVAEEAIVNAMRVLYYRDARSSRNFSLAIIDKNTGLTFKKNLQVENMKWDFAKDIKGYGTQKI E585BB3B5D0C8E2C psb5_yeast Proteasome subunit beta type-5 PRE2 DOA3 PRG1 YPR103W P8283.10 Macropain subunit PRE2 Proteinase YSCE subunit PRE2 Multicatalytic endopeptidase complex subunit PRE2 Proteasome component PRE2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MQAIADSFSVPNRLVKELQYDNEQNLESDFVTGASQFQRLAPSLTVPPIASPQQFLRAHTDDSRNPDCKIKIAHGTTTLAFRFQGGIIVAVDSRATAGNWVASQTVKKVIEINPFLLGTMAGGAADCQFWETWLGSQCRLHELREKERISVAAASKILSNLVYQYKGAGLSMGTMICGYTRKEGPTIYYVDSDGTRLKGDIFCVGSGQTFAYGVLDSNYKWDLSVEDALYLGKRSILAAAHRDAYSGGSVNLYHVTEDGWIYHGNHDVGELFWKVKEEEGSFNNVIG D0EBAC611F7A4F37 psa5_yeast Proteasome subunit alpha type-5 PUP2 DOA5 YGR253C G9155 Proteasome component PUP2 Macropain subunit PUP2 Proteinase YSCE subunit PUP2 Multicatalytic endopeptidase complex subunit PUP2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFLTRSEYDRGVSTFSPEGRLFQVEYSLEAIKLGSTAIGIATKEGVVLGVEKRATSPLLESDSIEKIVEIDRHIGCAMSGLTADARSMIEHARTAAVTHNLYYDEDINVESLTQSVCDLALRFGEGASGEERLMSRPFGVALLIAGHDADDGYQLFHAEPSGTFYRYNAKAIGSGSEGAQAELLNEWHSSLTLKEAELLVLKILKQVMEEKLDENNAQLSCITKQDGFKIYDNEKTAELIKELKEKEAAESPEEADVEMS E8EDEB212BF4EC6E q95z11_9tryp RRP45 protein protein 9tryp Trypanosoma brucei MQYQRAALVIASPALTQREVEFTKSAWLAGLRPDQRTAQQMREVSVEFPLLSRDVVVVRCGGSVVTAAIATALVEPTPQRPKHGLLHFSVRRLHTEKDSPAADGSTQKNLVAFLERLVRTGGVIDTAGLCVLPGQKVWSLTVNVTIMNDEGNCNDAAVWAVIALLMHHRRPELTVRGSSVIVHPPHEREPVPLSVQHTPLPFTFAITMAPTEREKALNHQRALSATGSSSGPQPSSGVGNSIDTASAFAIDGTSPQPLGVVVDPTTAESLAAASSIVVAINAEGQVCTVVKAEGCSIHLKDLNACIEAAGTLAPRVLDLIKVAMEAHDERRKTAMKGQFAWAQKRSGVGKVEQTCVKKIKTET q95z13_9tryp RRP41A protein protein 9tryp Trypanosoma brucei MPVIKEYINPAGLRLDGRRQNEPRRLALEFGKISGCDGCCTVVSGLSHVCATVYGPREVSNRLDGKYNEVTITCDVVVAAFAGERRREQQRYSRLSEDISASVLDVARSVVLLSQYPNSQIHICIEVLKQDGSDKAACINAACLALIDASIAMRDVVYTQTVGLIEGFDVVDLTTEEIHSQCPSICITVQGHDPTNIVWMESNSRLAPEVITRLVKVAQKASTDVFETALRGPLEEHAATVLRLQANLGNIE q8iew0_9tryp EAP4 protein protein 9tryp Trypanosoma brucei MERSHGRRSPDEVRYPHIVLNVLGQSYSSACIEVGSTRVVCAVHHPQQLIDEYRGSRGRVACTVRRSSRAQKHELAFRADVTPEKDLALALEGVVEQAVVLEKIPQLLVEVVVEILAEDGGLWDAIATGVCAALASGGFEMYDTFSACGAALLPDGSIVTDPSATEWNTAKATAVTCAMLNSGNMCFTAQRGSCEPTTALDLVTAALKGTLARKNVISSQLCEQFAF q4gyw4_tryb2 TbEAP1 TB927.1.2580 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MQFGPVVSEAEIRTVQDGIANGVRSDGRSLTQRRPFTVVTNRGSTTHFEGSSVEVHCDGTTVVAAATPSVVELDIDRGQAPRGSLFVNIDAVPAVVEHYAQSVRNNSARYRQAFLSFLATAIRQTFGAQGVTAEERQQQLGIAEAEIIADEGDDEQREDAFGGRGAAEEKPVEETAEQRVDCCGFPGEQLYIGGGYAFRIDVDAHVLQAHGGSLASIVALAVHAALKTVRLPSVTLHEASAGFSLEVNYSKPYATAVNWSRLPVLSVVHVSPTRHYVVDPTRLEELALPQQLRVAVNSFGQVFHMRFQQLPSRRGNAMRLIRSPREEAGKDNSNDNSERDVDGDDVDDSSRRGFDAGVKGTVGGMNMPDMAAVLHAAVHISQAVIAECDEALAGSSG q95z09_9tryp RRP41B protein protein 9tryp Trypanosoma brucei MATISRRDGRTAREMRGKELRTSDLSQFDGSAWYSQGLTAVTVAINGPTAARQENYRRCTTCIHVNRAARIPQEGGTGRLIMMERRERERCEDGELKQFLTSLVEAIVCLDRFPRCVLEAHVTVLMDDGALFAVASNAIMCALLDAGVPCRSTIAAVSLIMCNPEASGNEGEPEIFLDPTAVEESTDEVRSYASATFVLTNTGGEAVLASRVQARPRSSAYSRPITPKDLSRMFALAAKAAETLFVFFRNCSMALE q8mpg3_9tryp Ribosomal RNA-processing protein 43 EAP2 protein protein 9tryp Trypanosoma brucei MSLPPATGAVELASFQQHVHQFLVQNKRLDGRPLDALREPHILRDSKGGDGLLASTLYTDNTGMCLNCTVSGVFGPPPSDQPDEGRLTVDVAAPFFQSHSTDQVAAALREVARFVRSTIISCMDLTELNIINGEACWVLQVELVILNADGGLRAAALHAVVAAIHNMLLPRSRLPNGDEVESRRLRFHTIPVAVTIGVYKLGASGVRFLMDTNAAEESVVDGLLTVVLSDENGIITFVQSGSYSISSLLLTGAVNEFALRSAALRAAILKPSVAAAKINGSKNSVTDPHPLKEG q95z16_9tryp RRP4 protein protein 9tryp Trypanosoma brucei MSVPTCITGDTICGSAGPSKLDYVEDHVFLRGYNTYEGNNPNGAAAAQEGGGEIIASINGMVEVTDRVVSVKGMSSRYVAEVGDIVVGRVKEVCGNRWRVDVGAFQDAVMPLANVTEPGGILRRRGRSDELTMRNIFDEGELVVAEVQRVSQDGLISLHTRSGEKYGKLSDFGLLVLISPALVKRVKHHFMCLEFIHVNLVLGTNGAIWVSPATATTATESAKSAYDRFDADIRRAVTRVANCIRVMGNARFPVFDKSIEAAVKASIDFVLGPFEILYPGNQNLITTTVLDTINTRKRTIY q95z10_9tryp RRP6 protein protein 9tryp Trypanosoma brucei MSGSLPQTKDLVNGAFTLVKEYTKLTADLAEEDYDYHMAFPAFRHQITRHSEAVVSLMDKFCQLLPAKRRVTLSSTGGKLTDPQRAAVMEAADSLLENVDGLLDELKGRRLSAKDQLSVKFGSELQGVVPSSSYSVFNAAGVSVLRPQLTFEHPVDNSPTPFRPVYYDEKGVRHVGEPGVHPFAERIKAVSVPSEQLLLKTETPYLSLVTCPLTFVDTVEDLEALVAVLLNETEIAVDLEHHDFYSYQGFTCLMQISTRTQDFIVDCLKVRANMYLMAPVFLQPNIVKVFHGAREDVRWLQKDFGLYIVNLFDTSIALQNLHMPHSLAFAVDHFCQVKLNKKYQTADWRVRPIPAEMVSYAQQDTHFLLYVYDRLKQLLLNCEARASVGNMLLHVFQESRLLSLERYEKPHLDPDVTYKQALGRSLGGLSSPQLQVAREIFNWRDMAAREADDSPSAVMHISCVLSIATKLPTSANEVLKCCSPVSVAVRTNVMKLLQIVKDAIGSADSLKDGANGDVKPKPLPNVGVALRYMGVHRSMTGTLPSIEQRADPAFTVEDAHVVVRTEPSAWFETMRNVAAILRRKPYYPIALPGHDVVTRMSAVKRAREVEEMKVKGEGEEEEKRVHVDAEEPTVERENSSERGEGMAGPCLQEGLEVKDEVEEIDVPTQKPLELKEGAISLHQQYGTGSANRKKARLGKKNKL q95z17_9tryp CSL4 protein protein 9tryp Trypanosoma brucei MAVIVKTGKCVSPGDVLYATDIYDAIGTIVVAASDTGAVSAGNHADDAIIAGTGCYSRVVSKPDGAVSSQIITTLLGIVQWSGNVVSVRQPTKRARDATTIEVVSLNALKDAGTCGDSATPRLSTPGEATSSVSETPASTAVSTAPKSLWISTSVFGPRMGDNVHLRVVRVSRSFAYGEIIAVNGTWCSNSGSNGGGGGALGGFRGVIRMEDIRPFKPRKDQLTPPPPADAFQDGDVVVATVLSQSDVRQYQLSTVAEHCGVVEAFTVLNSNGRERRVRLRHIPECRDIMKCPLSGNLHRRWCPLIRLV q95z14_9tryp RRP40 protein protein 9tryp Trypanosoma brucei MSETNPVVRGAVGVHELAPLCSHVCLPGESVLLLEPTAVVTLGSGLHPLQQDGSDDRKESFEASGEEGSTVVVSEFCGPVLRTAGPNHTQLYKLNGPAARRYMYAARDPVVAIVMKKNASYYSCYTGAASLAVLDALGFDGATKTNKPRLQEGDVVYAFVKGNADASSLDTKFDDGRVVVSSSDEVELSCMAAEVGLVARDWTSAEAVFGPLVGGTVVRVALPYARSLIDGPASRLLALLGDRVPYDVCVGVNGLVWVRGHPSAADPTAAARRTVAVAACVVEAQDAATLEEMEAHVRGYFPKE q387l4_tryb2 Tb11.03.0760 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MTDVDGGSGASWKRLRFFVCADTLLTFQPVDSSHLEGRPGQPEQRYLLVDGEYRPITSYPTSSLFLETLKLAGSTVVVIAPWKRAKTKKLLSLFGWTAFDEIRSIHPEPGRDKLHLACIGCSADEYSTSVIVATSRADWSPSMYPQLVEVKHGVNQVPHSLALLRALSSCLRLAEWQVRLPHVPISSCATLCRSRLFSHCRFFLEPVVSEDATVALSINEHGGYIVTRREDATHCVVSGSLPNLPGDISNLPPGGCAAKAVKECSVLPTSAEGRSAEPKSAKVDTDSVSLVKNEVDESDDSIKDEFSYSAGNAATAMVECGDEGVWNEENADDEDPVTMIPPGTFSEDDEDDAPGGLEEADGETVTTVTTQWLKDCAEGLFMYPPQVSHTSKDGSDWNITGLLLATILPTSDAEPLLLSSFNTFAERSGFEPLQLNSADEGGLAEVMQRQWGGVLCMEEKDGDFLLSQTNIYTEAFSDEMDKHLTFVANRFCEAWKDAVQRRRALLGSDAGTQTTVSITVTFGTNTEVEVAPSVQPPPPPPPAPQPTTASAAANEGSKESATPALPFLMRALGPAEWAKEVEAPRSPPPSAVPDIPLKQDPVPSGEGVATAENSVGNDQKESTSRQVAGDAGDTANVAEATEEIAALDQPFEKCFIPTEALGSDREGLDRTQLERQLPFRNYPIKLNVSKSGIFCQFPTVSDAKRFYEEGTVEILNRSLPIKPVFEKRNETVAPAERKRRRSVSPGGVHPQTAAVSALSRRGIQGATYARTQRSRTDNGWKAEGDRNPFVLTEEENSLLTSLGITYTYALENVPLLEHYAQKDLSSKVYRKEKGVLVKMLEKLKAVKGTTSKAPQ q4vhg6_9tryp TRF protein protein 9tryp Trypanosoma brucei MYCHAGVPSTSPSPTDFSEVHKILCGHIKQGNNFSVAAAVALEPLHGSSEFWRTCGYRSGIVSALLASSLGVQRETDQLSHLEPMTVADVYAMFRHVVDENAHLRQIIARLESGECDPDDGSGGITSSAERLRSLISEGYDALTGRSSPCMGEDDCGQTPGKWKTCSSGAAGTKRRRDSPEVTERAYVRPESPVPASETLSPAANSGDDTVDEAEQNNALDVLEQLPALPGTTRTVSSFDAVGEMVNRGAGGPSLHPTNPSEFRVGSERGSTIETHSSSELSNFANVGVGTSSGKQKRYKFSASEDEAIIKGLARFTKGQQRFQQIYYAYRSVWHPARTVSQLYDHWRGTLRYKVIQQQGYRGKNSVAARSPEKASNMENNE gnd7-506_trybr_grna Trypanosoma brucei guide RNA gND7-506 (I and II) Stem/Loops I and II rna ribonucleic acid RNA rna 9tryp Trypanosoma brucei AAAUACGAUGUAAAUAACCUGUAGUAUAGUUAGUGUAUA p90629_9tryp gBP21 protein protein 9tryp Trypanosoma brucei MIRLACLRGPAMRSAWSRGASTFSGVQSLPKFEIHDVRDDPALGTMTRVAVDGKLLLISQYPQLGPRKVDPNDLSPQFDADRRISVRLRHVDLAYLVGVCKERVPRHRMETKAYTLDFEKSAQGYHLHGKVHRVASQRMEDWSVKFDNHFAVTLEHFLESALDESFGFRQHYATRAAEGGEKIAATSSAEGGARRKRSVSDTSRYH q952g2_9tryp protein protein 9tryp Trypanosoma brucei MLRRIISQQTTLIRKPLGAALFATSALRLEAASSSDADGKEVGSSGEGNRATGGKWRRPSLAQQRARRAQLPPAFDVVHWNDEDISRGHLLRVLHRDTFVVLDYHRQARMLTEEGNKAERVVSVMLPAVYTARFLAVLEGRSEKVEVHSRYTNATFTPNPAAPYTFTLKCTSTRPAQQKQQVAGEEGDETFEWTVEFDVAESLMLQRFLTQALHYNTGFARTSV p90629-mrp2 509643 N/A 509645 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 110 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 125 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 204 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 9tryp Trypanosoma brucei Stoichiometry: 1.0 N/A 509645 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 110 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 125 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 204 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 9tryp Trypanosoma brucei Stoichiometry: 1.0 N/A 509646 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 46 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 109 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 139 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 9tryp Trypanosoma brucei Stoichiometry: 2.0 direct interaction direct interaction false false false The sequence of the protein MRP1 used here has a Glu instead of a Leu at position 43, unlike the Uniprot sequence (P90629). 1, Table 1 The MRP1 sequence used had the N-terminal mito-targeting sequence and a C-terminal region found to cause insolubility removed. The MRP2 sequence used had the N-terminal mito-targeting sequence removed. The model includes 8 acetate molecules and 572 water molecules. p90629-mrp2-1 509643 N/A 509646 unspecified role unspecified role neutral component neutral component 46 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 109 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 139 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 9tryp Trypanosoma brucei Stoichiometry: 2.0 N/A 509645 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 110 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 125 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 204 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 9tryp Trypanosoma brucei Stoichiometry: 1.0 N/A 509645 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 110 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 125 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 204 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 9tryp Trypanosoma brucei Stoichiometry: 1.0 direct interaction direct interaction false false false The sequence of the protein MRP1 used here has a Glu instead of a Leu at position 43, unlike the Uniprot sequence (P90629). 1, Table 1 The MRP1 sequence used had the N-terminal mito-targeting sequence and a C-terminal region found to cause insolubility removed. The MRP2 sequence used had the N-terminal mito-targeting sequence removed. p90629-mrp2-2 509643 N/A 509646 unspecified role unspecified role neutral component neutral component 46 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 109 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 139 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false 9tryp Trypanosoma brucei Stoichiometry: 1.0 N/A 509647 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 N/A 509645 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false 110 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 125 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 204 se(s)met L-selenomethionine Delta:S(-1)Se(1) Se(S)Met Selenium replaces sulphur semetm certain certain sequence position certain certain certain sequence position certain false 9tryp Trypanosoma brucei Stoichiometry: 1.0 direct interaction direct interaction false false false The sequence of the protein MRP1 used here has a Glu instead of a Leu at position 43, unlike the Uniprot sequence (P90629). The MRP1 sequence used had the N-terminal mito-targeting sequence and a C-terminal region found to cause insolubility removed. The MRP2 sequence used had the N-terminal mito-targeting sequence removed. The heterotetramer, identical to that in the apo structures, was generated by crystallographic symmetry. 5 p90629-mrp2-3 509644 N/A 509647 unspecified role unspecified role neutral component neutral component region stem/loop II (oligo i) sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false fluorescein 5'-fluorescein tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 509646 unspecified role unspecified role neutral component neutral component 9tryp Trypanosoma brucei N/A 509645 unspecified role unspecified role neutral component neutral component 9tryp Trypanosoma brucei physical association physical association false false false The sequence of the protein MRP1 used here has a Glu instead of a Leu at position 43, unlike the Uniprot sequence (P90629). The MRP1 sequence used had the N-terminal mito-targeting sequence and a C-terminal region found to cause insolubility removed. The MRP2 sequence used had the N-terminal mito-targeting sequence removed. Only weak binding was observed to the oligo(U)-tail (Kd approx. 150 nM) and essentially no binding to stem/loop I alone, except when these molecules were heated to unfold them. 3 B p90629-mrp2-4 509644 N/A 509647 unspecified role unspecified role neutral component neutral component region stem/loopI-stem/loopII (oligo II) sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false fluorescein 5'-fluorescein tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 509646 unspecified role unspecified role neutral component neutral component 9tryp Trypanosoma brucei N/A 509645 unspecified role unspecified role neutral component neutral component 9tryp Trypanosoma brucei physical association physical association false false false The sequence of the protein MRP1 used here has a Glu instead of a Leu at position 43, unlike the Uniprot sequence (P90629). The MRP1 sequence used had the N-terminal mito-targeting sequence and a C-terminal region found to cause insolubility removed. The MRP2 sequence used had the N-terminal mito-targeting sequence removed. Only weak binding was observed to the oligo(U)-tail (Kd approx. 150 nM) and essentially no binding to stem/loop I alone, except when these molecules were heated to unfold them. The oligonucleotide was 5'-fluorescinated. Increasing the salt concentration decreased the binding and at concentrations of NaCl above 0.2 M binding is quickly abrogated. (Kds - 5.1 E-9 at 0.05 M NaCl, 8.5 E-9 M at 0.1 M NaCl, 2.7 E-8 M in 0.15 M NaCl, 2.06 E-8 in 0.2 M NaCl and no saturable binding at 0.3 and 0.4 M NaCl.) 3 B, C, S1 Magnesium is not required for high affinity binding (Kd 6.9 E-9 in the presence of 5.0 E-3 M magnesium ions versus 5.1 E-3 M in its absence). p90629-mrp2-5 509644 N/A 509647 unspecified role unspecified role neutral component neutral component region stem/loop I sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false fluorescein 5'-fluorescein tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) N/A 509646 unspecified role unspecified role neutral component neutral component 9tryp Trypanosoma brucei N/A 509645 unspecified role unspecified role neutral component neutral component 9tryp Trypanosoma brucei physical association physical association false false false The sequence of the protein MRP1 used here has a Glu instead of a Leu at position 43, unlike the Uniprot sequence (P90629). The MRP1 sequence used had the N-terminal mito-targeting sequence and a C-terminal region found to cause insolubility removed. The MRP2 sequence used had the N-terminal mito-targeting sequence removed. The oligonucleotide was 5'-fluorescinated. S3 B The RNA molecule was heated to 100 degrees C to unfold it. rrp45-rrp41a-1 Identification of an interaction between RRP45 and RRP41A of Trypanosoma brucei by Y2H 134448 N/A 134450 unspecified role unspecified role bait bait N/A 134451 unspecified role unspecified role prey prey physical association physical association false false false rrp45-rrp41a-2 Identification of an interaction between RRP45 and RRP41A of Trypanosoma brucei by Y2H 134448 N/A 134451 unspecified role unspecified role bait bait N/A 134450 unspecified role unspecified role prey prey physical association physical association false false false rrp-45-rrp41b-1 Identification of an interaction between RRP45 and RRP41B of Trypanosoma brucei by Y2H 134448 N/A 134450 unspecified role unspecified role bait bait N/A 134452 unspecified role unspecified role prey prey physical association physical association false false false rrp45-rrp41b-2 Identification of an interaction between RRP45 and RRP41B of Trypanosoma brucei by Y2H 134448 N/A 134450 unspecified role unspecified role prey prey N/A 134452 unspecified role unspecified role bait bait physical association physical association false false false eap2-41b-1 Identification of an interaction between EAP2 and RRP41B of Trypanosoma brucei by Y2H 134448 N/A 134452 unspecified role unspecified role bait bait N/A 134453 unspecified role unspecified role prey prey physical association physical association false false false eap2-rrp41b-2 Identification of an interaction between EAP2 and RRP41B of Trypanosoma brucei by Y2H 134448 N/A 134453 unspecified role unspecified role bait bait N/A 134452 unspecified role unspecified role prey prey physical association physical association false false false rrp41b-eap4-1 Identification of an interaction between EAP4 and RRP41B of Trypanosoma brucei by Y2H 134448 N/A 134454 unspecified role unspecified role prey prey N/A 134452 unspecified role unspecified role bait bait physical association physical association false false false rrp41b-eap4-2 Identification of an interaction between EAP4 and RRP41B of Trypanosoma brucei by Y2H 134448 N/A 134454 unspecified role unspecified role bait bait N/A 134452 unspecified role unspecified role prey prey physical association physical association false false false eap1-eap4-1 Identification of an interaction between EAP1 and EAP4 of Trypanosoma brucei by Y2H 134448 N/A 134454 unspecified role unspecified role prey prey N/A 134455 unspecified role unspecified role bait bait physical association physical association false false false eap1-eap4-2 Identification of an interaction between EAP1 and EAP4 of Trypanosoma brucei by Y2H 134448 N/A 134454 unspecified role unspecified role bait bait N/A 134455 unspecified role unspecified role prey prey physical association physical association false false false csl4-rrp41a-1 Identification of an interaction between CSL4 and RRP41A of Trypanosoma brucei by Y2H 134448 N/A 134451 unspecified role unspecified role bait bait N/A 134456 unspecified role unspecified role prey prey physical association physical association false false false Bait/prey role arbitarily assigned since not clear in paper csl4-eap4-1 Identification of an interaction between CSL4 and EAP4 of Trypanosoma brucei by Y2H 134448 N/A 134456 unspecified role unspecified role bait bait N/A 134454 unspecified role unspecified role prey prey physical association physical association false false false Bait/prey roles arbitarily assigned since not clear in paper rrp40-rrp4-1 Identification of an interaction between RRP40 and RRP4 of Trypanosoma brucei by Y2H 134448 N/A 134457 unspecified role unspecified role bait bait N/A 134458 unspecified role unspecified role prey prey physical association physical association false false false Bait/prey roles arbitarily assigned since not clear in paper rrp40-csl4-1 Identification of an interaction between RRP40 and CSL4 of Trypanosoma brucei by Y2H 134448 N/A 134457 unspecified role unspecified role bait bait N/A 134456 unspecified role unspecified role prey prey physical association physical association false false false Bait/prey roles arbitarily assigned since not clear in paper rrp4-csl4 Identification of the components of the Trypanosoma brucei exosome by TAP 134449 N/A 134458 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 134459 unspecified role unspecified role prey prey N/A 134456 unspecified role unspecified role prey prey N/A 134457 unspecified role unspecified role prey prey N/A 134455 unspecified role unspecified role prey prey N/A 134450 unspecified role unspecified role prey prey N/A 134451 unspecified role unspecified role prey prey N/A 134452 unspecified role unspecified role prey prey N/A 134454 unspecified role unspecified role prey prey N/A 134453 unspecified role unspecified role prey prey association association false false false An eleventh protein EAP3 (EMBL - AC091553) also seen but protein product not yet in UniProt pre1-pre2-1 Crystal structure of the 20s proteasome from yeast in complex with the proteasome activator pa26 from trypanosome brucei at 3.2 angstroms resolution 512208 N/A 512209 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512210 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) Stoichiometry: 14.0 N/A 512211 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512212 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512213 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512214 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512215 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512216 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512217 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512218 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512219 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512220 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512221 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512222 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 N/A 512223 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast Stoichiometry: 2.0 physical association physical association false false false 42meric, multimeric working 74.500%, free 67.500% 3.2A snap50 PTP tagging reveals SNAP50 interacting proteins. 357389 N/A 357390 unspecified role unspecified role prey prey N/A 357391 unspecified role unspecified role prey prey N/A 357392 unspecified role unspecified role prey prey N/A 357393 unspecified role unspecified role prey prey N/A 357394 unspecified role unspecified role prey prey N/A 357395 unspecified role unspecified role bait bait n terminal ProtC-TEV-ProtA tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false association association false false false snap50-1 PTP tagging reveals SNAP50 interacting proteins. 357389 N/A 357394 unspecified role unspecified role prey prey N/A 357390 unspecified role unspecified role prey prey N/A 357391 unspecified role unspecified role prey prey N/A 357392 unspecified role unspecified role prey prey N/A 357393 unspecified role unspecified role prey prey N/A 357395 unspecified role unspecified role bait bait c terminal ProtC-TEV-ProtA tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false snap50-2 PTP tagging reveals SNAP50 interacting proteins. 357389 N/A 357391 unspecified role unspecified role prey prey N/A 357392 unspecified role unspecified role prey prey N/A 357395 unspecified role unspecified role bait bait c terminal tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false rpa1 PTP tagging reveals RPA1 interacting proteins. 357389 N/A 357396 unspecified role unspecified role prey prey N/A 357397 unspecified role unspecified role prey prey N/A 357398 unspecified role unspecified role prey prey N/A 357399 unspecified role unspecified role prey prey N/A 357400 unspecified role unspecified role prey prey N/A 357401 unspecified role unspecified role prey prey N/A 357402 unspecified role unspecified role bait bait c terminal ProtC-TEV-ProtA tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false association association false false false tfall-1 PTP tagging reveals TFAII-1 interacting proteins. 357389 N/A 357390 unspecified role unspecified role prey prey N/A 357391 unspecified role unspecified role bait bait c terminal ProtC-TEV-ProtA tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 357392 unspecified role unspecified role prey prey N/A 357393 unspecified role unspecified role prey prey N/A 357394 unspecified role unspecified role prey prey N/A 357395 unspecified role unspecified role prey prey association association false false false trb-rap1-2 Binding of Trb to Rap1 (Tb11.03.0760) demonstrated by Y2H 814498 n/a 814503 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 814504 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 814509 814507 physical association physical association false false false Fig. 2A trb-trb-1 Homodimerisation of Trb by Y2H 814498 n/a 814503 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 814503 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 814514 814512 physical association physical association false false false Fig. 2A trb-rap1-1 814499 n/a 814504 unspecified role unspecified role prey prey n/a 814503 unspecified role unspecified role bait bait physical association physical association false false false trb-rap1-3 Binding of Trb to Rap1 (Tb11.03.0760) demonstrated by coimmunopreciptiation 814500 n/a 814504 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false n/a 814503 unspecified role unspecified role prey prey physical association physical association false false false Fig. 2B trb-rap1-4 Binding of Trb to Rap1 (Tb11.03.0760) demonstrated by coimmunopreciptiation 814501 n/a 814503 unspecified role unspecified role bait bait n/a 814504 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false Fig. 2B trb-rap1-5 Colocalisation of Trb and Rap1 (Tb11.03.0760) 814502 n/a 814503 unspecified role unspecified role neutral component neutral component n/a 814504 unspecified role unspecified role neutral component neutral component ty1 region tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false colocalization colocalization false false false Fig. 2C MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it http://mint.bio.uniroma2.it/mint wang-2006o-1 Sm core variation in spliceosomal small nuclear ribonucleoproteins from Trypanosoma brucei. in vitro In vitro pull down pull down predetermined predetermined participant predetermined Wang P., Palfi Z., Preusser C., Lücke S., Lane WS., Kambach C., Bindereif A. EMBO J. (0261-4189) 2006 2009-01-08 Only protein-protein interactions imex curation q6qa17_9tryp Lsm5 protein protein 9tryp Trypanosoma brucei MSVKSERRSFLSFLGCRVSVDLDDGSTLVGRLVSFSPTSNLILTDAERRKPLKRRRGSIRNECYNCVLFVRGSSVLSVKHSSGVTTDATVIDSITGRQTVVSKTIQAASQSLDTPLR mint q9gyz5_9tryp Sm-D1 protein protein 9tryp Trypanosoma brucei MPAAESSTLIGFLQQLRGTQVEIEMMNSTIVTGEITFVEANMNTYMRHVKITAKGKNPEMAETYMVCGSKIRYVILPEAMNTDDVLVKAAAAKARPGSKRTERSNE mint q38ba6_tryb2 Tb10.70.2250 protein protein tryb2 Trypanosoma brucei brucei (strain 927/4 GUTat10.1) MEGVADIPLRAICEATGFLVKVETTDGSVYDGKLMQLDAICGDIELIDVRCQHRDSSLSVEGRVFLKGSSVRLVHLPSDLKRAPFLDWKNLSIQKQLKSSLKASKGGENKWPKRTRMKPTKLEHRKKKLLL mint q9gyz6_9tryp Sm-G protein protein 9tryp Trypanosoma brucei MPAKKRIANLNHFMEKRVKVKLTSGRSISGELRGVDEFMSIVLYDAVDETPTSGTVENEDKTSLGTAVVRGAMIVDIVGLEV mint lsm5-sm_d1 544473 q6qa17_9tryp 544474 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 q9gyz5_9tryp 544475 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey purified purified flag tagged flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 direct interaction direct interaction false false false F3A mint lsm5-lsm5 544473 q6qa17_9tryp 544474 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 q6qa17_9tryp 544474 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey purified purified flag tagged flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 direct interaction direct interaction false false false F3B mint q38ba6-lsm5 544473 q6qa17_9tryp 544474 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey purified purified flag tagged flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 q38ba6_9tryp 544476 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 direct interaction direct interaction false false false F3B mint q38ba6-sm_g 544473 q38ba6_9tryp 544476 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role bait bait purified purified gst tagged gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 q9gyz6_9tryp 544477 nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence unspecified role unspecified role prey prey purified purified flag tagged flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Stoichiometry: 1.0 direct interaction direct interaction false false false F3A mint