IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ hale-2009-3 RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex. pyrfu Pyrococcus furiosus chromatography chromatography technology column chromatography chromatography de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Hale CR., Zhao P., Olson S., Duff MO., Graveley BR., Wells L., Terns RM., Terns MP. Archaea - Interaction dataset based on Archaea proteins Gel filtration and affinity chromatography followed by native gel electrophoresis rterns@bmb.uga.edu, mterns@bmb.uga.edu Cell (0092-8674) 2009 Only protein-protein interactions 2 imex curation trm1_pyrfu tRNA (guanine(26)-N(2))-dimethyltransferase trm1 tRNA(guanine-26,N(2)-N(2)) methyltransferase tRNA 2,2-dimethylguanosine-26 methyltransferase tRNA(m(2,2)G26)dimethyltransferase PF1871 protein protein pyrfu Pyrococcus furiosus MSMELFEVHEGKAKVLVPKAKTIYDSPVFYNPRMAPNRDVVVLLLNVLKPKIVLDALSATGIRGIRFALETPAEEIWMNDINELAYELMKKNVLLNFKGTLKENAKRAIFEGEKTIVINNDDANRLMAEKHRYFHFIDLDPFGSPMEFLDTALRSVKRKGILGVTATDGAPLCGAHPKACLRKYLAVPLRGELCHEVGTRILVGVIARYAAKYDLGMEVLLAYYKDHYFRAFVKLKDGAKKGDETLENLGYVYFDEKTGRFEVEKSFLSTRPNAYGPLWLGPLKNEKVVGEMLELLVSGFEVANYREVLKLLHMLHEELDIPLFYDTHALGKRLKIEPKKLGEIIKELKSMGYEATRTHFSPTGIKTNAPYEVFVEVMRKN q8u1g3_pyrfu PF1245 protein protein pyrfu Pyrococcus furiosus MRNLRPLDLTEKKKKKVRIYFEGKELEAYEGEKLPVALLANGIYWLTTSLEGRKRGAFTFGPVPMIINGVKGVDARKTKVKDGMKVQRQTYGDFHEEPLPQDGEVKQVVVDVLVIGGGPAGLGAVLEMQEHLNVALVEEKGWLGGDMFLKTSTAEGFEESSRKVVDKLAKEVKANVYLGTVALGVFDKGEYFLVPATKGNNLIEFLAKRVVLATGAVDNIMLFENNDMPGVFRRDFALEVMNVWEVAPGWNVAVTGSKAEEVIYELERWGIDYVEVPSVKRVEGKEKVEKVIDFNGNEYKVDAIIFADGKRPDINPITQAGGKLHFRRGYYRPVVNEYNQIREGVYVAGSAVTIKPHYTNYLEGRLVGAYILREFGIDSEPCIYKEKLKEFEPEALPVPKIPINKLNLDDVQICGCDVSLRKVYDVVEKGITDLQIIKRLTHLAMGFCQGRFCLFNGAAVVSQVTGIKLGEIDLPVARPPIKTVKLGILSRR q8u0n8_pyrfu PF1548 protein protein pyrfu Pyrococcus furiosus MSESINNITKPKERRDFVVMAGMRKDGTIDFIKVYALNEKLAIEVLEAFLKENNIHPSDFIVIQRGYEDVKDKKAITTRSEEELSAMLGRLGLRLVSNGVLYTDGIDKLYQITAISRELFESLQKEKREIFEDVQEKITFNFSKVDLPEKYVKKLRLLELMEDTIIFNMAELEIPNLLKAIVEGTVLIPRFLEKEDLIIRIFDEELHEYRGSYFDKVLIKPPIIHWDFYLDSLEDFSFKKVEESIYIAPLFLRATGGFLILTEPPEDLVKTLLKLKKRGEVRTILEGKRITIPINFTLIVDTRHPERYAGLKFPIRINLPPLDDETFLKVLETNLGITPPTEIVRIFPPDYKTFLGVELIKNLFEKLKLTEKGKDEVSLLKEAATIITGGTP q8u487_pyrfu Glutamine amidotransferase type-2 domain-containing protein PF0204 protein protein pyrfu Pyrococcus furiosus MLKYRDEKDISACGIFGIMDRKGRRFNGKLVIEAMSQMKVRGNGLGAGFAVYGLYKKFKEYYAFHVMIYDKDGREKLEKFLSTNFDIVYVDEIPVNDKAKVIDPPKFMRYFVAPRKKGTEKVLNDDDFVVKKVMYINRAIPGVYVISSGKNMGVFKGVGFPEDIADYFMIEEYKGYIWTAHTRFPTNTPGWWGGAHPFSILDWSVVHNGEISSYGTNKRYLEMYGYYSTLQTDTEVITYIFDLLVRRQGLPIEVVSKILAPPMWEEIDRMPEKEKKFYKALRMVYSPILLNGPWAIIVARHGEMIGLTDRIRLRPLTVAEKKSFLIISSEEAPIRYLFRDVDRIYTPYGGELVVGKLEEDIKKFVGESQ y1931_pyrfu UPF0273 protein PF1931 PF1931 protein protein pyrfu Pyrococcus furiosus MVRRVKTGIPGMDEILHGGIPERNVVLLSGGPGTGKSIFSQQFLWNGLQMGEPGIYVALEEHPVQVRQNMAQFGWDVRKYEEEGLFAMVDAFTAGVGKSKEYEKYIVHDLTDIREFIEVLRQAIRDINAKRVVVDSVTTLYINKPAMARSIILQLKRVLAGTGCTSIFVSQISVGERGFGGPGVEHGVDGIIRLDLDEIDGELKRSLIVWKMRGTSHSMKRHPFDITDKGIIVYPDKVLKRGRIYEL q8u4i5_pyrfu KaiC domain-containing protein PF0102 protein protein pyrfu Pyrococcus furiosus MPLQPIRRVKSGIPGFDELIEGGFPEGTTVLLTGGTGTGKTTFAAQFIYKGAEEYGEPGVFVTLEERAKDLRREMAAFGWDFEKYEKEGKIAIIDGVSSIAGIPSEEKFVLEDRFNVENFLRYVYRVVKAINAKRLVIDSIPSIAMRLEEENRIREVLLKLNTILLEMGVTTILTTEAPDPQHGRISRYGIEEFIARGVVILDLQERNIELKRYILIRKMRGTRHSMKKYPFEIGPNGIVVYPSGEIY q8tzz6_pyrfu PF1827 protein protein pyrfu Pyrococcus furiosus MKVKTIMTPNPVTITLPATRNYALELFRKHKVRSFPVVNKEGKLVGIISIKRVLTNVDEEQLAMLVKRDVPTVKPDDTLKKAAKLMLEYDYRRVVVIDEEGKPVGILTVGDIIRRYLAKSEKYKDVEIEPYYQRYVSVVWRGTPLMAALKALLLSNAMALPVIDDDGKLIGIVDETDLLRDSEIVRIMKSTELAASSEEEWILESHPTLLFEKFELKLPNKPVEDIMTRDVIVATPHMTVHEVARKMVKYKIEQLPVIRGDGDLVGLIRDFDLLKVLVK cmr6_pyrfu CRISPR system Cmr subunit Cmr6 cmr6 CRISPR type III-B/RAMP module RAMP protein Cmr6 PF1124 protein protein pyrfu Pyrococcus furiosus MKEVVKLVLLGERQNSLNLSLYFNKYPPTIIYPEVLEDRNKKLASPSGSQRKISLLVLNQGVLQFNKIKETIEKSLPIETKVKLPQKAYELYKKYYQDYTDMLNSLHAITGKFKTQSRLVVGLGDESVYETSIRLLRNYGVPYIPGSAIKGVTRHLTYYVLAEFINEGNDFYKRAKTVQDAFMKGDPKEILSNAKVPERCSRLCKEFLRIFGEKKVPEIIDELIRIFGTQKKEGEVVFFDAIPIAEEIADKPILELDIMNPHYGPYYQSGEKNVPPPGDWYDPIPIFFLTVPKDVPFLVAVGGRDRELTEKAFSLVKLALRDLGVGAKTSLGYGRLVEYV smc_pyrfu Chromosome partition protein Smc PF1843 smc protein protein pyrfu Pyrococcus furiosus MPYIEKLELKGFKSYGNKKVVIPFSKGFTAIVGANGSGKSNIGDAILFVLGGLSAKAMRASRISDLIFAGSKNEPPAKYAEVAIYFNNEDRGFPIDEDEVVIRRRVYPDGRSSYWLNGRRATRSEILDILTAAMISPDGYNIVLQGDITKFIKMSPLERRLLIDDISGIAEYDSKKEKALEELKQAEENLARVDLLIKEVKKQLDKLEKERNDALRYLDLKDKLEKAKVSLLLGEIKILETQIKEGEKRRAEIEEEIQKIEKEIEKIGKEIVEKVKVLREIEERIEKESGEEAIQITKKIGEVTSKIELTKRNIEVAKEELEDAQRRLAKTKEELRKVLSEIEKSKGAITRWKKRRDALINEIKKKEEERNVLVVKLGEIDKTFGAAREEFDSVVKELEETTRKMYEIEGNIRRLQEEKEKLHSRILFLRAKLPGIKEKINEFKAVVEDKRAEISEIEGKLSTIQAKRIKVEKEIEAKSNELEKVSKELESSERELIAAEAQREVRGNRAAEELKRSGIGGIYGTLAELIKVKDEAYALAIEVALGNRADNVVVEDELVAEKAIKYLKEHKLGRLTFLPLNKIKPKHVDSSVGLPAVDVIEYDQKIENAVKFALGDTVIVNSMEEARPHIGKVRMVTIEGELYERSGAITGGHFRARGLAVDTTKLREKVESLRRRKEALEGELNSLKIELRSLENASFELRIKLSDEKKELELASKDLNRLLEEENAVKEEIEESERKIQEIEQKIENEKSELAKLRGRIQRLERKKEKLKKALENPEARELMEKIRIIDGEISSLKEELSRIESRIESLESRLNEELLPRKASLEEEIEGLVNKINALKNNISENEKALELLNKELEKLKSIEENIKGEIRTLREKRKKLEEDISKLREKKEVLQRKLQELEIEANTLKVRDAQLNAQLEEKKYQLTHYDKNLIKSIKEIPLDLEKVKKEIEKMEEEIRSLEPVNMKAIEDFEIVERRYLELKSKREKLEAEKESIIEFINEIEKEKKNVFMRTFEAISRNFSEIFAKLSPGGSARLILENPEDPFSGGLEIEAKPAGKDVKRIEAMSGGEKALTALAFVFAIQKFKPAPFYLFDEIDAHLDDANVKRVADLIKESSKESQFIVITLRDVMMANADKIIGVSMRDGVSKVVSLSLEKAMKILEEIRKKQGWEHGN cmr4_pyrfu CRISPR system Cmr endoribonuclease Cmr4 cmr4 CRISPR type III-B/RAMP module RAMP protein Cmr4 Target RNase Cmr4 PF1126 protein protein pyrfu Pyrococcus furiosus MKAYLVGLYTLTPTHPGSGTELGVVDQPIQRERHTGFPVIWGQSLKGVLRSYLKLVEKVDEEKINKIFGPPTEKAHEQAGLISVGDAKILFFPVRSLKGVYAYVTSPLVLNRFKRDLELAGVKNFQTEIPELTDTAIASEEITVDNKVILEEFAILIQKDDKGILESVVKAIEQAFGNEMAEKIKGRIAIIPDDVFRDLVELSTEIVARIRINAETGTVETGGLWYEEYIPSDTLFYSLILVTPRAKDNDMALIKEVLGKINGKYLQIGGNETVGKGFVKVTLKEVTNNGGTHAK cmr2_pyrfu CRISPR system Cmr subunit Cmr2 cmr2 CRISPR-associated protein Cas10/Cmr2, subtype III-B PF1129 protein protein pyrfu Pyrococcus furiosus MVNIKEKLFVYLHDPPDKALKIENHEERSKKILSSGNIQYSRTDKVKQADALSSKTQRFIIRTKENKEPVIDFLGRSSGKYFHVGYPVFIHPISTEIKRYETLEKYIDLGRSNRGERFVNEFLERVSKLEGDVLKEVFEDASNKFKGEESKQWAYIWQFYPVKLKEGVKEFAKSELKLKEEEAEKFAEEFVNLPADTRFPDHAIWTHLDLTSALSVKDPTLLRIKIVPVQPFIANSRKQLDLWASSHLLSMLMYKALEVIVDKFGPEHVIYPSLRDQPFFLKFYLGENIGDEILVANLPNKALAIVSGKEAEKIEEEIKKRIRDFLLQLYREAVDWAVENGVVKVDRSEKDSMLKEAYLKIVREYFTVSITWVSLSEKEDIYQVTENAGLSDEDVKKWLKFAEKKENSRVLERIAIYPLLVKILDSLGERKVTEERFEKSEQLKGWKCHVCGENLAIFGDMYDHDNLKSLWLDEEPLCPMCLIKRYYPVWIRSKTGQKIRFESVVDVALLYKNWRKIFDEKYGKDLVSKAREVSEDFVKDNMLVDSDLYYSSTWESGLSKKLKNKKEIDEEKVKEVVDFLNAAYKEIGNPPKYYAILVMDGDDMGKVISGEVLGEISTRIHPNIRDYVEIPEAKYYSTPQVHVAISQALANFSIREVRSVVKDEGLLIYAGGDDVLAILPVDKALEVAYKIRKEFGKSFENGSLLPGWKLSAGILIVHYKHPLYDALEKARDLLNNKAKNVPGKDTLAIGLLKRSGSYYISLVGWELIRVFYNSELRKKLLEEKGGVGKRFIYHVLREVDTWPKVGIDEMLKFEVIRHIRGRNKEETKELREKIYGEIKDLLEHVRGNNEVEKVRGLFTFLKIITDAEVFP cmr3_pyrfu CRISPR system Cmr subunit Cmr3 cmr3 CRISPR type III-B/RAMP module-associated protein Cmr3 PF1128 protein protein pyrfu Pyrococcus furiosus MIEVTFTPYDVLLFRESRPFDAGSESVARSIIPLPQTVAGAIRTLLFYKGLKNCVGVGEEEPEFTLVGIAIGTEKGRIYPLPFNIIKSEKFYKVVNPGRFLGKLILPPKGKYKSGYVTESILEKYLKGELKEVEENKVIRIEKEKRIGIKLSREKKVVEEGMLYTVEFLRIEKIYAWIEDPGCGIKDILSSYEFLTLGGESRVAFVEVDDKTPDIFNRELGSTKKALFYFSTPTIGKVGEIVQELEKRLNAKIDDYLLVSSRPTAISGWDMHEKKPKGTKFAIPPGSVLFVEFKEEVEVPPYIKLGKLKKLGYGLALGGIWE q9p9k5_9eury protein protein 9eury Pyrococcus furiosus MVRRIRRWDIWDPFDLIREIQEEIDAMFDEFFSRPRLWTYRRWSEPAMYEERVGEVWREPFVDIFDNGDEFVITAELPGVRKEDIKVRVTEDTVYIEATVKREKELEREGAVRIERYFTGYRRAIRLPEEVIPEKAKAKYNNGVLEIRVPKKHPTKKESEGFEVKVE rad50_pyrfu DNA double-strand break repair Rad50 ATPase rad50 pfRad50 PF1167 protein protein pyrfu Pyrococcus furiosus MKLERVTVKNFRSHSDTVVEFKEGINLIIGQNGSGKSSLLDAILVGLYWPLRIKDIKKDEFTKVGARDTYIDLIFEKDGTKYRITRRFLKGYSSGEIHAMKRLVGNEWKHVTEPSSKAISAFMEKLIPYNIFLNAIYIRQGQIDAILESDEAREKVVREVLNLDKFETAYKKLSELKKTINNRIKEYRDILARTENIEELIKENEQELIQVLQEISKIEEVLPSKRSKVDMLRKEVLRLEETKVEIENSERLLEKRRGDKRTLEERIKNTEEYLEKLKEKEKELEEQVKEITSIKKDVDAYLALKEFKNEYLDKKYKIEKELTRVEELINEIQKRIEELNEKESEKEKLENEKKEILNKLAILEKDHQLYEEIKAKKENLRQLKEKLGDKSPEDIKKLLEELETKKTTIEEERNEITQRIGELKNKIGDLKTAIEELKKAKGKCPVCGRELTDEHREELLSKYHLDLNNSKNTLAKLIDRKSELERELRRIDMEIKRLTPLLTVAEQIRSIEEELNVVNLEKIEKNATEYEKLLEELRTLEGRIRGLAEDLKKLAPLEKKLAALIHKKQELEKELKELNTKLESFGFKSVEDLDSKLRELEEIYKRYLTLLNSKKELEITQREIAKAKETLEMSFEELAEVEADIERIEKKLSQLKQKYNEEEYKKKREEKEELEKELARLEAQKKELEKRRDTIKSTLEKLKAEKENRERVKKEIKDLEKAKDFTEELIEKVKKYKALAREAALSKIGELASEIFAEFTEGKYSEVVVRAEENKVRLFVVWEGKERPLTFLSGGERIALGLAFRLAMSLYLAGEISLLILDEPTPYLDEERRRKLITIMERYLKKIPQVILVSHDEELKDAADHVIRISLENGSSKVEVVS q8u4c6_pyrfu PF0162 protein protein pyrfu Pyrococcus furiosus MEINEFISQLKLGETVLVEHSSYSKPELLFYGIIKESRMPVVIDDILDTLFEYYNKLKVWGYDLTVFDKVYVIKAGGNEVIGNLLGKLEVGKYVIEAREYTKIIKEVNPPIINPVLGVHKLILLGNLLENLRLLKEISTYVGNENRVAIYFVNRDVVESHSPAALKLLEEISTSVFQLNEENKIKRMK pf1130-pf1128-2 Affinity purification by hydroxy apatite chromatography 866714 n/a 866762 unspecified role unspecified role neutral component neutral component n/a 866763 unspecified role unspecified role neutral component neutral component n/a 866764 unspecified role unspecified role neutral component neutral component n/a 866765 unspecified role unspecified role neutral component neutral component n/a 866766 unspecified role unspecified role neutral component neutral component n/a 866767 unspecified role unspecified role neutral component neutral component n/a 866751 unspecified role unspecified role neutral component neutral component n/a 866768 unspecified role unspecified role neutral component neutral component n/a 866769 unspecified role unspecified role neutral component neutral component n/a 866756 unspecified role unspecified role neutral component neutral component n/a 866770 unspecified role unspecified role neutral component neutral component n/a 866745 unspecified role unspecified role neutral component neutral component n/a 866749 unspecified role unspecified role neutral component neutral component n/a 866747 unspecified role unspecified role neutral component neutral component n/a 866746 unspecified role unspecified role neutral component neutral component association association false false false Supp. Table 1