IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ jiang-2021-1 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins Mammalian two-hybrid assay; Vectors -Bait (DNA binding Domain) uses plasmid "pM" and Prey (DNA Activation domain) plasmid VP16 with Luciferase Reporter. panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Cell & bioscience 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus jiang-2021-2 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Cell & bioscience 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus jiang-2021-3 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Cell & bioscience 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus jiang-2021-4 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Cell & bioscience 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus jiang-2021-5 in vitro In vitro mst microscale thermophoresis mst predetermined predetermined participant predetermined Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Cell & bioscience 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus jiang-2021-6 ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Jiang Y., Tong K., Yao R., Zhou Y., Lin H., Du L., Jin Y., Cao L., Tan J., Zhang XD., Guo D., Pan JA., Peng X. Virus - Publications including interactions involving viral proteins panjan@mail.sysu.edu.cn, pengxx9@mail.sysu.edu.cn Cell & bioscience 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus p0dtd1-pro_0000449621 Papain-like protease nsp3 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV APTKVTFGDDTVIEVQGYKSVNITFELDERIDKVLNEKCSAYTVELGTEVNEFACVVADAVIKTLQPVSELLTPLGIDLDEWSMATYYLFDESGEFKLASHMYCSFYPPDEDEEEGDCEEEEFEPSTQYEYGTEDDYQGKPLEFGATSAALQPEEEQEEDWLDDDSQQTVGQQDGSEDNQTTTIQTIVEVQPQLEMELTPVVQTIEVNSFSGYLKLTDNVYIKNADIVEEAKKVKPTVVVNAANVYLKHGGGVAGALNKATNNAMQVESDDYIATNGPLKVGGSCVLSGHNLAKHCLHVVGPNVNKGEDIQLLKSAYENFNQHEVLLAPLLSAGIFGADPIHSLRVCVDTVRTNVYLAVFDKNLYDKLVSSFLEMKSEKQVEQKIAEIPKEEVKPFITESKPSVEQRKQDDKKIKACVEEVTTTLEETKFLTENLLLYIDINGNLHPDSATLVSDIDITFLKKDAPYIVGDVVQEGVLTAVVIPTKKAGGTTEMLAKALRKVPTDNYITTYPGQGLNGYTVEEAKTVLKKCKSAFYILPSIISNEKQEILGTVSWNLREMLAHAEETRKLMPVCVETKAIVSTIQRKYKGIKIQEGVVDYGARFYFYTSKTTVASLINTLNDLNETLVTMPLGYVTHGLNLEEAARYMRSLKVPATVSVSSPDAVTAYNGYLTSSSKTPEEHFIETISLAGSYKDWSYSGQSTQLGIEFLKRGDKSVYYTSNPTTFHLDGEVITFDNLKTLLSLREVRTIKVFTTVDNINLHTQVVDMSMTYGQQFGPTYLDGADVTKIKPHNSHEGKTFYVLPNDDTLRVEAFEYYHTTDPSFLGRYMSALNHTKKWKYPQVNGLTSIKWADNNCYLATALLTLQQIELKFNPPALQDAYYRARAGEAANFCALILAYCNKTVGELGDVRETMSYLFQHANLDSCKRVLNVVCKTCGQQQTTLKGVEAVMYMGTLSYEQFKKGVQIPCTCGKQATKYLVQQESPFVMMSAPPAQYELKHGTFTCASEYTGNYQCGHYKHITSKETLYCIDGALLTKSSEYKGPITDVFYKENSYTTTIKPVTYKLDGVVCTEIDPKLDNYYKKDNSYFTEQPIDLVPNQPYPNASFDNFKFVCDNIKFADDLNQLTGYKKPASRELKVTFFPDLNGDVVAIDYKHYTPSFKKGAKLLHKPIVWHVNNATNKATYKPNTWCIRCLWSTKPVETSNSFDVLKSEDAQGMDNLACEDLKPVSEEVVENPTIQKDVLECNVKTTEVVGDIILKPANNSLKITEEVGHTDLMAAYVDNSSLTIKKPNELSRVLGLKTLATHGLAAVNSVPWDTIANYAKPFLNKVVSTTTNIVTRCLNRVCTNYMPYFFTLLLQLCTFTRSTNSRIKASMPTTIAKNTVKSVGKFCLEASFNYLKSPNFSKLINIIIWFLLLSVCLGSLIYSTAALGVLMSNLGMPSYCTGYREGYLNSTNVTIATYCTGSIPCSVCLSGLDSLDTYPSLETIQITISSFKWDLTAFGLVAEWFLAYILFTRFFYVLGLAAIMQLFFSYFAVHFISNSWLMWLIINLVQMAPISAMVRMYIFFASFYYVWKSYVHVVDGCNSSTCMMCYKRNRATRVECTTIVNGVRRSFYVYANGGKGFCKLHNWNCVNCDTFCAGSTFISDEVARDLSLQFKRPINPTDQSSYIVDSVTVKNGSIHLYFDKAGQKTYERHSLSHFVNLDNLRANNTKGSLPINVIVFDGKSKCEESSAKSASVYYSQLMCQPILLLDQALVSDVGDSAEVAVKMFDAYVNTFSSTFNVPMEKLKTLVATAEAELAKNVSLDNVLSTFISAARQGFVDSDVETKDVVECLKLSHQSDIEVTGDSCNNYMLTYNKVENMTPRDLGACIDCSARHINAQVAKSHNIALIWNVKDFMSLSEQLRKQIRSAAKKNNLPFKLTCATTRQVVNVVTTKIALKGG 819 2763 p0dtd1-pro_0000449619 Host translation inhibitor nsp1 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MESLVPGFNEKTHVQLSLPVLQVRDVLVRGFGDSVEEVLSEARQHLKDGTCGLVEVEKGVLPQLEQPYVFIKRSDARTAPHGHVMVELVAELEGIQYGRSGETLGVLVPHVGEIPVAYRKVLLRKNGNKGAGGHSYGADLKSFDLGDELGTDPYEDFQENWNTKHSSGVTRELMRELNGG 1 180 p0dtd1-pro_0000449629 RNA-directed RNA polymerase nsp12 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SADAQSFLNRVCGVSAARLTPCGTGTSTDVVYRAFDIYNDKVAGFAKFLKTNCCRFQEKDEDDNLIDSYFVVKRHTFSNYQHEETIYNLLKDCPAVAKHDFFKFRIDGDMVPHISRQRLTKYTMADLVYALRHFDEGNCDTLKEILVTYNCCDDDYFNKKDWYDFVENPDILRVYANLGERVRQALLKTVQFCDAMRNAGIVGVLTLDNQDLNGNWYDFGDFIQTTPGSGVPVVDSYYSLLMPILTLTRALTAESHVDTDLTKPYIKWDLLKYDFTEERLKLFDRYFKYWDQTYHPNCVNCLDDRCILHCANFNVLFSTVFPPTSFGPLVRKIFVDGVPFVVSTGYHFRELGVVHNQDVNLHSSRLSFKELLVYAADPAMHAASGNLLLDKRTTCFSVAALTNNVAFQTVKPGNFNKDFYDFAVSKGFFKEGSSVELKHFFFAQDGNAAISDYDYYRYNLPTMCDIRQLLFVVEVVDKYFDCYDGGCINANQVIVNNLDKSAGFPFNKWGKARLYYDSMSYEDQDALFAYTKRNVIPTITQMNLKYAISAKNRARTVAGVSICSTMTNRQFHQKLLKSIAATRGATVVIGTSKFYGGWHNMLKTVYSDVENPHLMGWDYPKCDRAMPNMLRIMASLVLARKHTTCCSLSHRFYRLANECAQVLSEMVMCGGSLYVKPGGTSSGDATTAYANSVFNICQAVTANVNALLSTDGNKIADKYVRNLQHRLYECLYRNRDVDTDFVNEFYAYLRKHFSMMILSDDAVVCFNSTYASQGLVASIKNFKSVLYYQNNVFMSEAKCWTETDLTKGPHEFCSQHTMLVKQGDDYVYLPYPDPSRILGAGCFVDDIVKTDGTLMIERFVSLAIDAYPLTKHPNQEYADVFHLYLQYIRKLHDELTGHMLDMYSVMLTNDNTSRYWEPEFYEAMYTPHTVLQ 4393 5324 p0dtd1-pro_0000449631 Proofreading exoribonuclease nsp14 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AENVTGLFKDCSKVITGLHPTQAPTHLSVDTKFKTEGLCVDIPGIPKDMTYRRLISMMGFKMNYQVNGYPNMFITREEAIRHVRAWIGFDVEGCHATREAVGTNLPLQLGFSTGVNLVAVPTGYVDTPNNTDFSRVSAKPPPGDQFKHLIPLMYKGLPWNVVRIKIVQMLSDTLKNLSDRVVFVLWAHGFELTSMKYFVKIGPERTCCLCDRRATCFSTASDTYACWHHSIGFDYVYNPFMIDVQQWGFTGNLQSNHDLYCQVHGNAHVASCDAIMTRCLAVHECFVKRVDWTIEYPIIGDELKINAACRKVQHMVVKAALLADKFPVLHDIGNPKAIKCVPQADVEWKFYDAQPCSDKAYKIEELFYSYATHSDKFTDGVCLFWNCNVDRYPANSIVCRFDTRVLSNLNLPGCDGGSLYVNKHAFHTPAFDKSAFVNLKQLPFFYYSDSPCESHGKQVVSDIDYVPLKSATCITRCNLGGAVCRHHANEYRLYLDAYNMMISAGFSLWVYKQFDTYNLWNTFTRLQ 5926 6452 p0dtc2-pro_0000449648 Spike protein S2 S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT 686 1273 ns7a_sars2 ORF7a protein Accessory protein 7a Protein U122 Protein X4 7a protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MKIILFLALITLATCELYHYQECVRGTTVLLKEPCSSGTYEGNSPFHPLADNKFALTCFSTQFAFACPDGVKHVYQLRARSVSPKLFIRQEEVQELYSPIFLIVAAIVFITLCFTLKRKTE ns7b_sars2 ORF7b protein Accessory protein 7b 7b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MIELSLIDFYLCFLAFLLFLVLIMLIIFWFSLELQDHNETCHA ns8_sars2 ORF8 protein Non-structural protein 8 8 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MKFLVFLGIITTVAAFHQECSLQSCTQHQPYVVDDPCPIHFYSKWYIRVGARKSAPLIELCVDEAGSKSPIQYIDIGNYTVSCLPFTINCQEPKLGSLVVRCSFYEDFLEYHDVRVVLDFI ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA p0dtd1-pro_0000449620 Non-structural protein 2 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AYTRYVDNNFCGPDGYPLECIKDLLARAGKASCTLSEQLDFIDTKRGVYCCREHEHEIAWYTERSEKSYELQTPFEIKLAKKFDTFNGECPNFVFPLNSIIKTIQPRVEKKKLDGFMGRIRSVYPVASPNECNQMCLSTLMKCDHCGETSWQTGDFVKATCEFCGTENLTKEGATTCGYLPQNAVVKIYCPACHNSEVGPEHSLAEYHNESGLKTILRKGGRTIAFGGCVFSYVGCHNKCAYWVPRASANIGCNHTGVVGEGSEGLNDNLLEILQKEKVNINIVGDFKLNEEIAIILASFSASTSAFVETVKGLDYKAFKQIVESCGNFKVTKGKAKKGAWNIGEQKSILSPLYAFASEAARVVRSIFSRTLETAQNSVRVLQKAAITILDGISQYSLRLIDAMMFTSDLATNNLVVMAYITGGVVQLTSQWLTNIFGTVYEKLKPVLDWLEEKFKEGVEFLRDGWEIVKFISTCACEIVGGQIVTCAKEIKESVQTFFKLVNKFLALCADSIIIGGAKLKALNLGETFVTHSKGLYRKCVKSREETGLLMPLKAPKEIIFLEGETLPTEVLTEEVVLKTGDLQPLEQPTSEAVEAPLVGTPVCINGLMLLEIKDTEKYCALAPNMMVTNNTFTLKGG 181 818 p0dtd1-pro_0000449622 Non-structural protein 4 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV KIVNNWLKQLIKVTLVFLFVAAIFYLITPVHVMSKHTDFSSEIIGYKAIDGGVTRDIASTDTCFANKHADFDTWFSQRGGSYTNDKACPLIAAVITREVGFVVPGLPGTILRTTNGDFLHFLPRVFSAVGNICYTPSKLIEYTDFATSACVLAAECTIFKDASGKPVPYCYDTNVLEGSVAYESLRPDTRYVLMDGSIIQFPNTYLEGSVRVVTTFDSEYCRHGTCERSEAGVCVSTSGRWVLNNDYYRSLPGVFCGVDAVNLLTNMFTPLIQPIGALDISASIVAGGIVAIVVTCLAYYFMRFRRAFGEYSHVVAFNTLLFLMSFTVLCLTPVYSFLPGVYSVIYLYLTFYLTNDVSFLAHIQWMVMFTPLVPFWITIAYIICISTKHFYWFFSNYLKRRVVFNGVSFSTFEEAALCTFLLNKEMYLKLRSDVLLPLTQYNRYLALYNKYKYFSGAMDTTSYREAACCHLAKALNDFSNSGSDVLYQPPQTSITSAVLQ 2764 3263 vemp_sars2 Envelope small membrane protein E 4 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MYSFVSEETGTLIVNSVLLFLAFVVFLLVTLAILTALRLCAYCCNIVNVSLVKPSFYVYSRVKNLNSSRVPDLLV ns6_sars2 ORF6 protein Accessory protein 6 Protein X3 6 Non-structural protein 6 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFHLVDFQVTIAEILLIIMRTFKVSIWNLDYIINLIIKNLSKSLTENKYSQLDEEQPMEID p0dtd1-pro_0000449623 3C-like proteinase nsp5 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SGFRKMAFPSGKVEGCMVQVTCGTTTLNGLWLDDVVYCPRHVICTSEDMLNPNYEDLLIRKSNHNFLVQAGNVQLRVIGHSMQNCVLKLKVDTANPKTPKYKFVRIQPGQTFSVLACYNGSPSGVYQCAMRPNFTIKGSFLNGSCGSVGFNIDYDCVSFCYMHHMELPTGVHAGTDLEGNFYGPFVDRQTAQAAGTDTTITVNVLAWLYAAVINGDRWFLNRFTTTLNDFNLVAMKYNYEPLTQDHVDILGPLSAQTGIAVLDMCASLKELLQNGMNGRTILGSALLEDEFTPFDVVRQCSGVTFQ 3264 3569 p0dtd1-pro_0000449624 Non-structural protein 6 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SAVKRTIKGTHHWLLLTILTSLLVLVQSTQWSLFFFLYENAFLPFAMGIIAMSAFAMMFVKHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLDMVDTSLSGFKLKDCVMYASAVVLLILMTARTVYDDGARRVWTLMNVLTLVYKVYYGNALDQAISMWALIISVTSNYSGVVTTVMFLARGIVFMCVEYCPIFFITGNTLQCIMLVYCFLGYFCTCYFGLFCLLNRYFRLTLGVYDYLVSTQEFRYMNSQGLLPPKNSIDAFKLNIKLLGVGGKPCIKVATVQ 3570 3859 p0dtd1-pro_0000449625 Non-structural protein 7 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SKMSDVKCTSVVLLSVLQQLRVESSSKLWAQCVQLHNDILLAKDTTEAFEKMVSLLSVLLSMQGAVDINKLCEEMLDNRATLQ 3860 3942 p0dtd1-pro_0000449627 Non-structural protein 9 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV NNELSPVALRQMSCAAGTTQTACTDDNALAYYNTTKGGRFVLALLSDLQDLKWARFPKSDGTGTIYTELEPPCRFVTDTPKGPKVKYLYFIKGLNNLNRGMVLGSLAATVRLQ 4141 4253 p0dtd1-pro_0000449628 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQ 4254 4392 p0dtd1-pro_0000449633 2'-O-methyltransferase nsp16 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SSQAWQPGVAMPNLYKMQRMLLEKCDLQNYGDSATLPKGIMMNVAKYTQLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDSDLNDFVSDADSTLIGDCATVHTANKWDLIISDMYDPKTKNVTKENDSKEGFFTYICGFIQQKLALGGSVAIKITEHSWNADLYKLMGHFAWWTAFVTNVNASSSEAFLIGCNYLGKPREQIDGYVMHANYIFWRNTNPIQLSSYSLFDMSKFPLKLRGTAVMSLKEGQINDMILSLLSKGRLIIRENNRVVISSDVLVNN 6799 7096 p0dtd1-pro_0000449626 Non-structural protein 8 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AIASEFSSLPSYAAFATAQEAYEQAVANGDSEVVLKKLKKSLNVAKSEFDRDAAMQRKLEKMADQAMTQMYKQARSEDKRAKVTSAMQTMLFTMLRKLDNDALNNIINNARDGCVPLNIIPLTTAAKLMVVIPDYNTYKNTCDGTTFTYASALWEIQQVVDADSKIVQLSEISMDNSPNLAWPLIVTALRANSAVKLQ 3943 4140 p0dtd1-pro_0000449630 Helicase nsp13 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AVGACVLCNSQTSLRCGACIRRPFLCCKCCYDHVISTSHKLVLSVNPYVCNAPGCDVTDVTQLYLGGMSYYCKSHKPPISFPLCANGQVFGLYKNTCVGSDNVTDFNAIATCDWTNAGDYILANTCTERLKLFAAETLKATEETFKLSYGIATVREVLSDRELHLSWEVGKPRPPLNRNYVFTGYRVTKNSKVQIGEYTFEKGDYGDAVVYRGTTTYKLNVGDYFVLTSHTVMPLSAPTLVPQEHYVRITGLYPTLNISDEFSSNVANYQKVGMQKYSTLQGPPGTGKSHFAIGLALYYPSARIVYTACSHAAVDALCEKALKYLPIDKCSRIIPARARVECFDKFKVNSTLEQYVFCTVNALPETTADIVVFDEISMATNYDLSVVNARLRAKHYVYIGDPAQLPAPRTLLTKGTLEPEYFNSVCRLMKTIGPDMFLGTCRRCPAEIVDTVSALVYDNKLKAHKDKSAQCFKMFYKGVITHDVSSAINRPQIGVVREFLTRNPAWRKAVFISPYNSQNAVASKILGLPTQTVDSSQGSEYDYVIFTQTTETAHSCNVNRFNVAITRAKVGILCIMSDRDLYDKLQFTSLEIPRRNVATLQ 5325 5925 p0dtd1-pro_0000449632 Uridylate-specific endoribonuclease nsp15 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVETFYPKLQ 6453 6798 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT ap3a_sars2 ORF3a protein Accessory protein 3a Protein U274 Protein X1 3a Protein 3a protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASLPFGWLIVGVALLAVFQSASKIITLKKRWQLALSKGVHFVCNLLLLFVTVYSHLLLVAAGLEAPFLYLYALVYFLQSINFVRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL vme1_sars2 Membrane protein E1 glycoprotein Matrix glycoprotein Membrane glycoprotein M protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MADSNGTITVEELKKLLEQWNLVIGFLFLTWICLLQFAYANRNRFLYIIKLIFLWLLWPVTLACFVLAAVYRINWITGGIAIAMACLVGLMWLSYFIASFRLFARTRSMWSFNPETNILLNVPLHGTILTRPLLESELVIGAVILRGHLRIAGHHLGRCDIKDLPKEITVATSRTLSYYKLGASQRVAGDSGFAAYSRYRIGNYKLNTDHSSSSDNIALLVQ a0a7g5x2w6_sars2 ORF10 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MGYINVFAFPFTIYSLLLCRMNSRNYIAQVDVVNFNLT rep-rep-224 4074316 N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4074323 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 1 Nsp1;Nsp3.1 rep-rep-223 4074316 N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4074323 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 2 Nsp1;Nsp3.2 rep-rep-225 4074316 N/A 4074324 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074323 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 3 Nsp1;Nsp12 rep-rep-226 4074316 N/A 4074325 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074323 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 4 Nsp1;Nsp14 rep-s-7 4074316 N/A 4074326 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074323 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 5 Nsp1;S2. S2 sequence deduced from nucleotide numbering in Table 1. rep-ns7a_sars2-2 4074316 N/A 4074327 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074323 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 6 Nsp1;Orf 7a. rep-ns7b_sars2-1 4074316 N/A 4074328 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074323 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 7 Nsp1;Orf 7b. rep-ns7b_sars2-2 4074316 N/A 4074329 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074323 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 8 Nsp1;Orf8. rep-n-2 4074316 N/A 4074330 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074323 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 9 Nsp1;N. rep-rep-227 4074316 N/A 4074331 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074323 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 10 Nsp2;Nsp1. rep-rep-228 4074316 N/A 4074331 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074325 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 11 Nsp2;Nsp14. rep-rep-229 4074316 N/A 4074322 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4074323 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 12 Nsp3.1;Nsp1. rep-rep-230 4074316 N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4074322 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B, Number 13 Nsp3.1;Nsp3.1. rep-rep-231 4074316 N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.3 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4074322 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B, Number 14 Nsp3.1;Nsp3.3. rep-rep-232 4074316 N/A 4074325 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074322 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B, Number 15 Nsp3.1;Nsp14. rep-n-3 4074316 N/A 4074330 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074322 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1 A/B, Number 16 Nsp3.1;N. rep-rep-233 4074316 N/A 4074324 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074322 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed 3.2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B, Number 17 Nsp3.2;Nsp12. rep-rep-234 4074316 N/A 4074323 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074332 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 18 Nsp4;Nsp1. rep-e-2 4074316 N/A 4074333 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074332 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 19 Nsp4;E. rep-ns6_sars2-4 4074316 N/A 4074334 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074332 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 20 Nsp4;Orf6. rep-rep-235 4074316 N/A 4074335 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074335 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 21 Nsp5;Nsp5. rep-rep-236 4074316 N/A 4074324 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074335 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 22 Nsp5;Nsp12. rep-rep-237 4074316 N/A 4074323 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074336 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 23 Nsp6;Nsp1. rep-rep-238 4074316 N/A 4074323 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074337 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 24 Nsp7;Nsp1. rep-rep-239 4074316 N/A 4074323 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074338 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 25 Nsp9;Nsp1. rep-ns8_sars2-1 4074316 N/A 4074329 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074338 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 26 Nsp9;Orf8. rep-rep-240 4074316 N/A 4074323 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074339 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 27 Nsp10;Nsp1. rep-rep-241 4074316 N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4074339 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 28 Nsp10;Nsp3.1. rep-rep-242 4074316 N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4074339 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 29 Nsp10;Nsp3.2. rep-rep-243 4074316 N/A 4074324 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074339 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 30 Nsp10;Nsp12. rep-rep-244 4074316 N/A 4074325 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074339 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 31 Nsp10;Nsp14. rep-rep-245 4074316 N/A 4074340 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074339 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 32 Nsp10;Nsp16. rep-e-3 4074316 N/A 4074333 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074339 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 33 Nsp10;E. rep-ns6_sars2-5 4074316 N/A 4074334 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074339 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 34 Nsp10;Orf6. rep-ns8_sars2-2 4074316 N/A 4074329 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074339 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 35 Nsp10;Orf8. rep-n-4 4074316 N/A 4074330 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074339 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 36 Nsp10;N. rep-rep-246 4074316 N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4074324 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 37 Nsp12;Nsp3.2. rep-rep-247 4074316 N/A 4074341 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074324 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 38 Nsp12;Nsp8. rep-rep-248 4074316 N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4074342 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 39 Nsp13;Nsp3.2. rep-rep-249 4074316 N/A 4074325 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074342 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 40 Nsp13;Nsp14. rep-rep-250 4074316 N/A 4074325 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074339 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 41 Nsp14;Nsp10. rep-rep-251 4074316 N/A 4074343 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074343 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 42 Nsp15;Nsp15. rep-rep-252 4074316 N/A 4074340 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074339 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 43 Nsp16;Nsp10. rep-rep-253 4074316 N/A 4074344 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed S1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4074323 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 44 S1;Nsp1. S1 sequence deduced from nucleotide numbering in Table 1. ap3a_sars2-rep-2 4074316 N/A 4074345 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B, Number 45 Orf3:Nsp3.1 Identity of Orf3 found from Table 1 stated position in polyprotein. ap3a_sars2-rep-3 4074316 N/A 4074346 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074323 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 46 M:Nsp1 vme1_sars2-rep-1 4074316 N/A 4074346 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074324 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 47 M:Nsp12 vme1_sars2-ns6_sars2-1 4074316 N/A 4074346 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074334 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 48 M:Orf6. n-rep-2 4074316 N/A 4074330 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B, Number 49 N:Nsp3.1. n-n-85 4074316 N/A 4074330 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074330 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 50 N:N. orf10-rep-3 4074316 N/A 4074347 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed 3.2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 1B, Number 51 Orf10:Nsp3.2 There is no Uniprot reviewed entry for Orf10;this may be because that the genome sequence currently annotated as orf10 may not have a protein coding function in SARS-CoV-2. A random Trembl entry has been used here, based on the nucleotide sequence numbers given in Table 1. orf10-rep-4 4074316 N/A 4074347 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 4074324 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 52 Orf10:Nsp12. There is no Uniprot reviewed entry for Orf10;this may be because that the genome sequence currently annotated as orf10 may not have a protein coding function in SARS-CoV-2. A random Trembl entry has been used here, based on the nucleotide sequence numbers given in Table 1. rep-ns8_sars2-3 4074316 N/A 4074329 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4074329 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false 1B, Number 53 Nsp9;Orf8. ns8_sars2-ns8_sars2-1 4074317 N/A 4074329 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074329 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2A ns8_sars2-ns8_sars2-2 4074317 N/A 4074330 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074330 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2B rep-rep-254 4074317 N/A 4074343 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074343 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2C rep-rep-255 4074317 N/A 4074335 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074335 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2D rep-rep-256 4074317 N/A 4074339 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074325 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2E rep-rep-257 4074317 N/A 4074339 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074340 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2F rep-n-5 4074317 N/A 4074330 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 2G rep-rep-258 4074317 N/A 4074341 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074324 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false physical association physical association false false false 2H rep-n-6 4074317 N/A 4074330 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false NTD sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3 B/C The N-terminal number for the "N" protein has been taken from Uniprot, as it does not appear to have been stated. Compared with N, NTD interacted with Nsp3.1 in a stronger manner. n-102 4074317 N/A 4074330 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 3 G/H n-103 4074317 N/A 4074330 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4 B/C/I n-104 4074317 N/A 4074330 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074322 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 3.1 region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 4 G/H n-rep-4 4074317 N/A 4074322 competitor competitor competitor prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4074330 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074322 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 6B n-101 4074318 N/A 4074330 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false NTD sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false N/A 4074322 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 3 D-F The N-terminal number for the "N" protein has been taken from Uniprot, as it does not appear to have been stated. n-rep-3 4074318 N/A 4074330 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4074322 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false 3.1 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false colocalization colocalization false false false 4 D-F Only aa 1-235 of Nsp3.1 plays an indispensable role in the interaction between Nsp3.1 and N. rep-n-7 4074319 N/A 4074330 unspecified role unspecified role prey prey partially purified partially purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 4074322 unspecified role unspecified role bait bait partially purified partially purified 3.1 region, partial sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false 5A rep-n-8 4074320 N/A 4074330 unspecified role unspecified role prey prey partially purified partially purified possible tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 4074322 unspecified role unspecified role bait bait partially purified partially purified region fluorescent dye fluorescent dye label fluorescent dye undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false possible tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false 3.1 region, partial sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) physical association physical association false false false 4074320 5B rep-n-9 4074321 N/A 4074330 unspecified role unspecified role neutral component neutral component purified purified NTD region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4074322 unspecified role unspecified role neutral component neutral component purified purified 3.1 region, partial sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false 5C-H