DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhou-2021-2 ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Zhou S., Bhukya H., Malet N., Harrison PJ., Rea D., Belousoff MJ., Venugopal H., Sydor PK., Styles KM., Song L., Cryle MJ., Alkhalaf LM., Fülöp V., Challis GL., Corre C. G.L.Challis@warwick.ac.uk; C.Corre@warwick.ac.uk Nature 2021 imex curation Only protein-protein interactions imex curation zhou-2021-3 ecoli Escherichia coli (strain K12) 3d-em-single electron microscopy 3D single particle reconstruction 3D-EM-single experimental particp experimental participant identification experimental particp Zhou S., Bhukya H., Malet N., Harrison PJ., Rea D., Belousoff MJ., Venugopal H., Sydor PK., Styles KM., Song L., Cryle MJ., Alkhalaf LM., Fülöp V., Challis GL., Corre C. G.L.Challis@warwick.ac.uk; C.Corre@warwick.ac.uk Nature 2021 imex curation Only protein-protein interactions imex curation q9jn89_strch mmfR protein protein strch Streptomyces coelicolor MTSAQQPTPFAVRSNVPRGPHPQQERSIKTRAQILEAASEIFASRGYRGASVKDVAERVGMTKGAVYFHFPSKESLAIAVVEEHYARWPAAMEEIRIQGFTPLETVEEMLHRAAQAFRDDPVMQAGARLQSERAFIDAELPLPYVDWTHLLEVPLQDAREAGQLRAGVDPAAAARSLVAAFFGMQHVSDNLHQRADIMERWQELRELMFFALRA mmf2 4-(Hydroxymethyl)-2-propylfuran-3-carboxylic acid bioactive entity bioactive entity bioactive entity chemical synthesis Chemical synthesis (Chemical synthesis) mmfr-1 4048748 N/A 4048750 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2, Ext. Data Fig. 6 mmf2-mmfr-1 4048748 N/A 4048750 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 4048751 unspecified role unspecified role neutral component neutral component Stoichiometry: 1.0 direct interaction direct interaction false false false Fig. 2, Ext. Data Fig. 6 mmfr-2 4048749 N/A 4048750 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false Fig. 2, Ext. Data Fig. 7 Two MmfR homodimers bind to opposite faces of the DNA duplex