DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ caffalette-2021-1 ecoli Escherichia coli (strain K12) 3d-em-single electron microscopy 3D single particle reconstruction 3D-EM-single experimental particp experimental participant identification experimental particp Caffalette CA., Zimmer J. jochen_zimmer@virginia.edu. Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation IMEx imex curation caffalette-2021-2 ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column experimental particp experimental participant identification experimental particp Caffalette CA., Zimmer J. jochen_zimmer@virginia.edu. Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation caffalette-2021-3 ecoli Escherichia coli (strain K12) comigration in sds comigration in sds page comigration in sds experimental particp experimental participant identification experimental particp Caffalette CA., Zimmer J. jochen_zimmer@virginia.edu. Proceedings of the National Academy of Sciences of the United States of America 2021 Only protein-protein interactions imex curation imex curation o67182_aquae Transport permease protein abcT3 aq_1095 protein protein aquae Aquifex aeolicus (strain VF5) MNLSLILELVRQEIKNRYADTVLGIWWAFLWPILLVLIYTLIFSHLIGAKLGHENTVYAYSIYLSSGIFPWFFFSNSLSRITGIFTEKKFLFTKIPIRLEVFPVVVIISELINYLIGISLVTLISFITLGFEGIKYFYLFPVALYLMIVYSFSIGMVLGTLNVFFRDIKEIIGVFLQIFFWFTPIVYTLDILPPFVKKLIYYNPMYPVVSIHHLVFVNYLDLHLYSLLGFLLASPLVFFVSYYFFKKLEKDIKDFA o67181_aquae abcT4 aq_1094 protein protein aquae Aquifex aeolicus (strain VF5) MMIRVFDVWKKYKYYKKPQDRLKEIIFRKPFHEELWVLKGINLEIEKGEVLGIVGPNGAGKSTLLKVITGVTEPDKGFVERSGKVVGLLELGTGFNYELSGLENIYVNASLLGLSRREIDEKLESIIEFSELDDFINKPLKTYSSGMIMRLAFSIAIHTEPECFIIDEALAVGDAHFQQKCFRKLKEHKQKGGSIIFVSHDMNAVKILCDRAILLHKGEIIEEGSPETVTQAYYKLMASLENKEGITFLQNGYGNFKAVIKEVRLKSEHGYTNNFPSGDTLFIELDVEAKEDLQDVVAGILIRDRFGQDIFGINTYLMEKKVELKKGKYLFTFKMPLNLAPGKYTLTVALHKGMDHAQECYHWIDNVCNFEVNGFKKEQFVGVCYLPTEFNYRKIP caffalette-2021-1 3976379 N/A 3976382 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 N/A 3976383 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified O67181:p.Glu168Gln E167Q mutation mutation mutation certain certain sequence position certain certain certain sequence position certain false His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig 1, Fig 2, Table S1 abct3-abct4-1 3976380 N/A 3976382 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified ecoli Escherichia coli (strain K12) N/A 3976383 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified O67181:p.Glu168Gln E167Q mutation mutation mutation certain certain sequence position certain certain certain sequence position certain false His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig S1a abct4-2 3976381 N/A 3976383 weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified O67181:p.Glu168Gln E167Q mutation mutation mutation certain certain sequence position certain certain certain sequence position certain false His Tag His Tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig S1a