IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ xiao-2021-1 in vitro In vitro 3d-em 3D electron microscopy 3D-EM electron tomog structure determination identification by structure determination identification by structure determination structure determination Xiao T., Lu J., Zhang J., Johnson RI., McKay LGA., Storm N., Lavine CL., Peng H., Cai Y., Rits-Volloch S., Lu S., Quinlan BD., Farzan M., Seaman MS., Griffiths A., Chen B. Virus - Publications including interactions involving viral proteins The authors have stated that the "m" in ACE2m-615-Fc stands for "mutated", with mutations in the peptidase active site. Peptidase activity assay; BioVision, the suppliers of the synthetic peptide substrate from the ACE2 activity kit have confirmed the sequence of the substrate was MCA-APK-(Dnp)-OH (Mca-Ala-Pro-Lys(Dnp)-OH) as in Catalog #: B2726. bchen@crystal.harvard.edu Nature structural & molecular biology 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus xiao-2021-2 in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Xiao T., Lu J., Zhang J., Johnson RI., McKay LGA., Storm N., Lavine CL., Peng H., Cai Y., Rits-Volloch S., Lu S., Quinlan BD., Farzan M., Seaman MS., Griffiths A., Chen B. Virus - Publications including interactions involving viral proteins The authors have stated that the "m" in ACE2m-615-Fc stands for "mutated", with mutations in the peptidase active site. Peptidase activity assay; BioVision, the suppliers of the synthetic peptide substrate from the ACE2 activity kit have confirmed the sequence of the substrate was MCA-APK-(Dnp)-OH (Mca-Ala-Pro-Lys(Dnp)-OH) as in Catalog #: B2726. bchen@crystal.harvard.edu Nature structural & molecular biology 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus xiao-2021-3 in vitro In vitro cleavage cleavage assay cleavage predetermined predetermined participant predetermined Xiao T., Lu J., Zhang J., Johnson RI., McKay LGA., Storm N., Lavine CL., Peng H., Cai Y., Rits-Volloch S., Lu S., Quinlan BD., Farzan M., Seaman MS., Griffiths A., Chen B. Virus - Publications including interactions involving viral proteins FRET based assay:On cleavage of the peptide by the protease, the quencher (DNP) and fluorophore (MCA) are separated giving a large increase in fluorescence. The Specific Activity was calculated as the amount of the released fluorophore divided by the reaction time and the amount of the ACE2 protein using the data within the initial linear phase. The authors have stated that the "m" in ACE2m-615-Fc stands for "mutated", with mutations in the peptidase active site. Peptidase activity assay; BioVision, the suppliers of the synthetic peptide substrate from the ACE2 activity kit have confirmed the sequence of the substrate was MCA-APK-(Dnp)-OH (Mca-Ala-Pro-Lys(Dnp)-OH) as in Catalog #: B2726. bchen@crystal.harvard.edu Nature structural & molecular biology 2021 Only protein-protein interactions imex curation imex curation Coronavirus - Interactions investigated in the context of Coronavirus ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT mca-apk-dnp-oh Mca-APK(Dnp)-OH peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) APK yes ace2-s-86 4049115 N/A 4049118 unspecified role unspecified role neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 3.0 N/A 4049119 unspecified role unspecified role neutral component neutral component purified purified P0DTC2:p.[Arg682_Arg685delinsGlyGlySerGly;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. Stoichiometry: 3.0 direct interaction direct interaction false false false Table 1 ace2-s-87 4049115 N/A 4049118 unspecified role unspecified role neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 2.0 N/A 4049119 unspecified role unspecified role neutral component neutral component purified purified P0DTC2:p.[Arg682_Arg685delinsGlyGlySerGly;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. Stoichiometry: 3.0 direct interaction direct interaction false false false Table 1 ace2-s-88 4049115 N/A 4049118 unspecified role unspecified role neutral component neutral component purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f Stoichiometry: 1.0 N/A 4049119 unspecified role unspecified role neutral component neutral component purified purified P0DTC2:p.[Arg682_Arg685delinsGlyGlySerGly;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. Stoichiometry: 3.0 direct interaction direct interaction false false false Table 1 ace2-s-89 4049115 N/A 4049119 unspecified role unspecified role neutral component neutral component purified purified P0DTC2:p.[Arg682_Arg685delinsGlyGlySerGly;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. Stoichiometry: 3.0 direct interaction direct interaction false false false Table 1 ace2-s-90 4049116 N/A 4049118 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. Stoichiometry: 3.0 N/A 4049119 unspecified role unspecified role bait bait purified purified P0DTC2:p.[Arg682_Arg685delinsGlyGlySerGly;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. Stoichiometry: 3.0 direct interaction direct interaction false false false 4049116 2B, Table 2+3. Curves for monomeric ACE2 fitted a 1:1 binding model. ACE2-615-a monomer. s-ace2-75 4049116 N/A 4049118 unspecified role unspecified role prey prey purified purified C-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. Stoichiometry: 2.0 N/A 4049119 unspecified role unspecified role bait bait purified purified P0DTC2:p.[Arg682_Arg685delinsGlyGlySerGly;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 4049116 2B, Table 2+3. ACE2-740-Fc - a dimer. Curves for oligomeric ACE2 variants fitted a bivalent binding model. The bivalent model describes the binding of a bivalent analyte to immobilized ligand, where one analyte molecule can bind to one or two ligand molecules. As a result of binding of one analyte molecule to two ligand sites, the overall binding is strengthened compared with 1:1 binding. s-ace2-76 4049116 N/A 4049118 unspecified role unspecified role prey prey purified purified C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false T4 phage fibritin trimerization motif and slightly longer linker (13 residues) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q9BYF1:p.Thr27Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false T4 phage fibritin trimerization motif with linker (11 residues) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q9BYF1:p.Thr27Trp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.His34Trp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Lys353Tyr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. Stoichiometry: 3.0 N/A 4049119 unspecified role unspecified role bait bait purified purified P0DTC2:p.[Arg682_Arg685delinsGlyGlySerGly;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 4049116 2B, Table 2+3, Extended data 7+8. ACE2-615-foldon- a trimer. Curves for oligomeric ACE2 variants fitted a bivalent binding model. The bivalent model describes the binding of a bivalent analyte to immobilized ligand, where one analyte molecule can bind to one or two ligand molecules. As a result of binding of one analyte molecule to two ligand sites, the overall binding is strengthened compared with 1:1 binding. s-ace2-77 4049116 N/A 4049118 unspecified role unspecified role prey prey purified purified C-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false Q9BYF1:p.[His374Asn;His378Asn] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. Stoichiometry: 2.0 N/A 4049119 unspecified role unspecified role bait bait purified purified P0DTC2:p.[Arg682_Arg685delinsGlyGlySerGly;Lys986_Val987delinsProPro] mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false T4 phage fibritin trimerization motif tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. direct interaction direct interaction false false false 4049116 Table 2+3. Extended Data 8. ACE2m-615-Fc -a dimer. Curves for oligomeric ACE2 variants fitted a bivalent binding model. The bivalent model describes the binding of a bivalent analyte to immobilized ligand, where one analyte molecule can bind to one or two ligand molecules. As a result of binding of one analyte molecule to two ligand sites, the overall binding is strengthened compared with 1:1 binding. ace2-mca_apk(dnp)_oh-1 4049117 N/A 4049118 enzyme enzyme neutral component neutral component C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. Stoichiometry: 1.0 N/A 4049120 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3A All the trimeric forms showed essentially the same specific activity; the monomeric ACE2615 and the dimeric ACE2740-Fc had lower specific activities. ACE2-615-a monomer. Specific Activity:4.54mU/ng ace2-mca_apk(dnp)_oh-2 4049117 N/A 4049118 enzyme enzyme neutral component neutral component purified purified C-terminus fc-igg tag fc-igg tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. Stoichiometry: 2.0 N/A 4049120 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3A All the trimeric forms showed essentially the same specific activity; the monomeric ACE2615 and the dimeric ACE2740-Fc had lower specific activities. ACE2-740-Fc - a dimer. Specific Activity:3.59 mU/ng ace2-mca_apk(dnp)_oh-3 4049117 N/A 4049118 enzyme enzyme neutral component neutral component purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false T4 phage fibritin trimerization motif and slightly longer linker (13 residues) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q9BYF1:p.Thr27Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain false T4 phage fibritin trimerization motif with linker (11 residues) tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q9BYF1:p.Thr27Trp mutation mutation certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.His34Trp mutation mutation certain certain sequence position certain certain certain sequence position certain false Q9BYF1:p.Lys353Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain false human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. Stoichiometry: 3.0 N/A 4049120 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false 3A All the trimeric forms showed essentially the same specific activity; the monomeric ACE2615 and the dimeric ACE2740-Fc had lower specific activities. ACE2-615-foldon- a trimer. Specific Activities:range 5.4 to 7.2 (K353Y)mU/ng.