DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ martin-2020-3 nicbe-leaf Nicotiana benthamiana leaf leaf leaf 3d-em-single electron microscopy 3D single particle reconstruction 3D-EM-single experimental particp experimental participant identification experimental particp Martin R., Qi T., Zhang H., Liu F., King M., Toth C., Nogales E., Staskawicz BJ. :enogales@lbl.gov, stask@berkeley.edu Science (New York, N.Y.) 2020 Only protein-protein interactions imex curation imex curation martin-2020-4 nicbe-leaf Nicotiana benthamiana leaf leaf leaf pull down pull down experimental particp experimental participant identification experimental particp Martin R., Qi T., Zhang H., Liu F., King M., Toth C., Nogales E., Staskawicz BJ. :enogales@lbl.gov, stask@berkeley.edu Science (New York, N.Y.) 2020 Only protein-protein interactions imex curation imex curation q5qa88_xaneu xopQ protein protein Xanthomonas euvesicatoria Xanthomonas euvesicatoria MQPTAIRSTVGLPGADMTADLRDPAPVAVPAHSAADAAAPPPGALQTIVGRPPRPDGPRHRRAQSLPARLTPAQRGMLAELGVADTSVLTPTETAVLRELRLHRPPLPLDTLLFTDPNKDPDDVVTYTIAKQLQAEGFLRLTDVVVTLGDADMRSQRAQLAKGVFDRLALPEVRVARGQDYPMTSTQAREHSKFLAEGAALRAAPDAVHTDGVRAMRERLATSPHKLGMVVIAGMTDASALLAEAGDLVREKLASITIMGGIDPARDADGLVQPDTRAYNNATDIHAARALYRRAQQLGIPLRILSKEAAYRAAVPPAFYEGIARNGHPVGEYLRDVQKNALKGLWEGIQANLIPGLDTAWFFRTFVAAQPQDPAAADQQGAMSFDAIWPQVTKLNLYDPLTLLAALPGAARLLFQPTPMHREGASPVEHVGHAEVVRPEKARLLLSALAKAALAQQDEGQRGR roq1_nicbe Disease resistance protein Roq1 NAD(+) hydrolase RPV1 Recognition of XopQ 1 protein ROQ1 protein protein nicbe Nicotiana benthamiana MLTSSSHHGRSYDVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFAVVVFSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVRKQNGEYAVCFTKFEANLVDDRDKVLRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCFSISITNRDLVGIESQIKKLSSLLRMDLKGVRLVGIWGMGGVGKTTAARALFNRYYQNFESACFLEDVKEYLQHHTLLYLQKTLLSKLLKVEFVDCTDTEEMCVILKRRLCSKKVLVVLDDVNHNDQLDKLVGAEDWFGSGSRIVITTRDMKLLKNHDVHETYEIKVLEKDEAIELFNLHAFKRSSPEKEFKELLNLVVDYTGGLPLALKVLGSLLYKEDLDVWISTIDRLKDNPEGEIMATLKISFDGLRDYEKSIFLDIACFFRGYNQRDMTALFHASGFHPVLGVKTLVEKSLIFILEDKIQMHDLMQEMGRQIAVQESPMRRIYRPEDVKDACIGDMRKEAIEGLLLTEPEQFEEGELEYMYSAEALKKTRRLRILVKEYYNRGFDEPVAYLPNSLLWLEWRNYSSNSFPSNFEPSKLVYLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTPDFRMITNLERLILSSCDALVEVHPSVGFLKNLILLNMDHCISLERLPAIIQSECLEVLDLNYCFNLKMFPEVERNMTHLKKLDLTSTGIRELPASIEHLSSLENLQMHSCNQLVSLPSSIWRFRNLKISECEKLGSLPEIHGNSNCTRELILKLVSIKELPTSIGNLTSLNFLEICNCKTISSLSSSIWGLTSLTTLKLLDCRKLKNLPGIPNAINHLSGHGLQLLLTLEQPTIYERLDLLRIIDMSWCSCISSLPHNIWMLKFLRILCISYCSRLEYLPENLGHLEHLEELLADGTGILRLPSSVARLNKLEVLSFRKKFAIGPKVQYSSSMLNLPDDVFGSLGSLGSVVKLNLSGNGFCNLPETMNQLFCLEYLDITFCQRLEALPELPPSIKELYVDEHLALRIMEDLVIKCKELNLIAVTKIEYQNFYRWLDSIWSDVSELLENSQKQQLDDMLQLIPFSYLSTAKREEVLKIVIHGTRIPEWFRWQDRSATTMSVNLPEYWYTENFLGFAICCSCCFYHSARSYDVEFEGSMHHYNYDSSYWKEYEEPSYDFYERDSIEITAKLTPRHKGMRTEELKKVCSFSMNVLRRATAVPNMCFAFFPFNSLCHISNLQANNPNDYGIFETCLSPGDIRHRGKQWGFNLVYKDETGGSVTHEMLINR roq1-xopq-1 3966061 N/A 3966063 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified StrepII tag StrepII tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false nicbe-leaf Nicotiana benthamiana leaf leaf leaf Stoichiometry: 1.0 N/A 3966064 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false nicbe-leaf Nicotiana benthamiana leaf leaf leaf Stoichiometry: 1.0 direct interaction direct interaction false false false Fig 1, Table S1 roq1-1 3966061 N/A 3966064 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed purified purified Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false nicbe-leaf Nicotiana benthamiana leaf leaf leaf Stoichiometry: 4.0 direct interaction direct interaction false false false Fig 1, Table S1 xopq-roq1-1 3966062 N/A 3966064 weight silver stain molecular weight estimation by silver staining weight silver stain predetermined predetermined participant predetermined unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false nicbe-leaf Nicotiana benthamiana leaf leaf leaf N/A 3966063 weight silver stain molecular weight estimation by silver staining weight silver stain predetermined predetermined participant predetermined unspecified role unspecified role bait bait over-expressed over expressed level over-expressed StrepII tag StrepII tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false nicbe-leaf Nicotiana benthamiana leaf leaf leaf physical association physical association false false false Fig S13