UniProt UniProt http://www.uniprot.org adamus-2020-3 yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. jpalecek@sci.muni.cz Journal of molecular biology 2020 Only protein-protein interactions imex curation imex curation adamus-2020-1 ecoli Escherichia coli (strain K12) crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. DSBU (disuccinimidyl dibutyric urea) crosslinker. jpalecek@sci.muni.cz Journal of molecular biology 2020 Only protein-protein interactions imex curation imex curation adamus-2020-4 in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. jpalecek@sci.muni.cz Journal of molecular biology 2020 Only protein-protein interactions imex curation imex curation adamus-2020-2 ecoli Escherichia coli (strain K12) tem transmission electron microscopy tem predetermined predetermined participant predetermined Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. jpalecek@sci.muni.cz Journal of molecular biology 2020 Only protein-protein interactions imex curation imex curation adamus-2020-5 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Adamus M., Lelkes E., Potesil D., Ganji SR., Kolesar P., Zabrady K., Zdrahal Z., Palecek JJ. jpalecek@sci.muni.cz Journal of molecular biology 2020 Only protein-protein interactions imex curation imex curation smc5_human Structural maintenance of chromosomes protein 5 SMC5 KIAA0594 SMC5L1 protein protein human Homo sapiens Human MATPSKKTSTPSPQPSKRALPRDPSSEVPSKRKNSAPQLPLLQSSGPFVEGSIVRISMENFLTYDICEVSPGPHLNMIVGANGTGKSSIVCAICLGLAGKPAFMGRADKVGFFVKRGCSRGMVEIELFRASGNLVITREIDVAKNQSFWFINKKSTTQKIVEEKVAALNIQVGNLCQFLPQDKVGEFAKLSKIELLEATEKSIGPPEMHKYHCELKNLREKEKQLETSCKEKTEYLQKMVQRNERYKQDVERFYERKRHLDLIEMLEAKRPWVEYENVRQEYEEVKLVRDRVKEEVRKLKEGQIPVTCRIEEMENERHNLEARIKEKATDIKEASQKCKQKQDVIERKDKHIEELQQALIVKQNEELDRQRRIGNTRKMIEDLQNELKTTENCENLQPQIDAITNDLRRIQDEKALCEGEIIDKRRERETLEKEKKSVDDHIVRFDNLMNQKEDKLRQRFRDTYDAVLWLRNNRDKFKQRVCEPIMLTINMKDNKNAKYIENHIPSNDLRAFVFESQEDMEVFLKEVRDNKKLRVNAVIAPKSSYADKAPSRSLNELKQYGFFSYLRELFDAPDPVMSYLCCQYHIHEVPVGTEKTRERIERVIQETRLKQIYTAEEKYVVKTSFYSNKVISSNTSLKVAQFLTVTVDLEQRRHLEEQLKEIHRKLQAVDSGLIALRETSKHLEHKDNELRQKKKELLERKTKKRQLEQKISSKLGSLKLMEQDTCNLEEEERKASTKIKEINVQKAKLVTELTNLIKICTSLHIQKVDLILQNTTVISEKNKLESDYMAASSQLRLTEQHFIELDENRQRLLQKCKELMKRARQVCNLGAEQTLPQEYQTQVPTIPNGHNSSLPMVFQDLPNTLDEIDALLTEERSRASCFTGLNPTIVQEYTKREEEIEQLTEELKGKKVELDQYRENISQVKERWLNPLKELVEKINEKFSNFFSSMQCAGEVDLHTENEEDYDKYGIRIRVKFRSSTQLHELTPHHQSGGERSVSTMLYLMALQELNRCPFRVVDEINQGMDPINERRVFEMVVNTACKENTSQYFFITPKLLQNLPYSEKMTVLFVYNGPHMLEPNTWNLKAFQRRRRRITFTQPS eid3_human EP300-interacting inhibitor of differentiation 3 EID-1-like inhibitor of differentiation 3 E1A-like inhibitor of differentiation 3 EID3 Non-structural maintenance of chromosomes element 4 homolog B protein protein human Homo sapiens Human MKMDVSVRAAGCSDDLSSGEADVDPKLLELTADEEKCRSIRRQYRQLMYCVRQNREDIVSSANNSLTEALEEANVLFDGVSRTREAALDARFLVMASDLGKEKAKQLNSDMNFFNQLAFCDFLFLFVGLNWMEGDPDKLSDCDDSIALSFWKAIEKEATSWMVKAETFHFVFGSFKLERSAPKPRLEHQKKVRKMEENGNMPTKLQKLDLSSYPEATEKNVERILGLLQTYFRKYPDTPVSYFEFVIDPNSFSRTVENIFYVSFIVRDGFARIRLDEDRLPILEPMNVNQMGEGNDSSCHGRKQGVISLTLQEWKNIVAAFEISEAMITYSSY nse2_human E3 SUMO-protein ligase NSE2 NSMCE2 C8orf36 MMS21 Non-structural maintenance of chromosomes element 2 homolog MMS21 homolog E3 SUMO-protein transferase NSE2 protein protein human Homo sapiens Human MPGRSSSNSGSTGFISFSGVESALSSLKNFQACINSGMDTASSVALDLVESQTEVSSEYSMDKAMVEFATLDRQLNHYVKAVQSTINHVKEERPEKIPDLKLLVEKKFLALQSKNSDADFQNNEKFVQFKQQLKELKKQCGLQADREADGTEGVDEDIIVTQSQTNFTCPITKEEMKKPVKNKVCGHTYEEDAIVRMIESRQKRKKKAYCPQIGCSHTDIRKSDLIQDEALRRAIENHNKKRHRHSE slf2_human SMC5-SMC6 complex localization factor protein 2 SLF2 C10orf6 FAM178A Smc5/6 localization factor 1 protein protein human Homo sapiens Human MTRRCMPARPGFPSSPAPGSSPPRCHLRPGSTAHAAAGKRTESPGDRKQSIIDFFKPASKQDRHMLDSPQKSNIKYGGSRLSITGTEQFERKLSSPKESKPKRVPPEKSPIIEAFMKGVKEHHEDHGIHESRRPCLSLASKYLAKGTNIYVPSSYHLPKEMKSLKKKHRSPERRKSLFIHENNEKNDRDRGKTNADSKKQTTVAEADIFNNSSRSLSSRSSLSRHHPEESPLGAKFQLSLASYCRERELKRLRKEQMEQRINSENSFSEASSLSLKSSIERKYKPRQEQRKQNDIIPGKNNLSNVENGHLSRKRSSSDSWEPTSAGSKQNKFPEKRKRNSVDSDLKSTRESMIPKARESFLEKRPDGPHQKEKFIKHIALKTPGDVLRLEDISKEPSDETDGSSAGLAPSNSGNSGHHSTRNSDQIQVAGTKETKMQKPHLPLSQEKSAIKKASNLQKNKTASSTTKEKETKLPLLSRVPSAGSSLVPLNAKNCALPVSKKDKERSSSKECSGHSTESTKHKEHKAKTNKADSNVSSGKISGGPLRSEYGTPTKSPPAALEVVPCIPSPAAPSDKAPSEGESSGNSNAGSSALKRKLRGDFDSDEESLGYNLDSDEEEETLKSLEEIMALNFNQTPAATGKPPALSKGLRSQSSDYTGHVHPGTYTNTLERLVKEMEDTQRLDELQKQLQEDIRQGRGIKSPIRIGEEDSTDDEDGLLEEHKEFLKKFSVTIDAIPDHHPGEEIFNFLNSGKIFNQYTLDLRDSGFIGQSAVEKLILKSGKTDQIFLTTQGFLTSAYHYVQCPVPVLKWLFRMMSVHTDCIVSVQILSTLMEITIRNDTFSDSPVWPWIPSLSDVAAVFFNMGIDFRSLFPLENLQPDFNEDYLVSETQTTSRGKESEDSSYKPIFSTLPETNILNVVKFLGLCTSIHPEGYQDREIMLLILMLFKMSLEKQLKQIPLVDFQSLLINLMKNIRDWNTKVPELCLGINELSSHPHNLLWLVQLVPNWTSRGRQLRQCLSLVIISKLLDEKHEDVPNASNLQVSVLHRYLVQMKPSDLLKKMVLKKKAEQPDGIIDDSLHLELEKQAYYLTYILLHLVGEVSCSHSFSSGQRKHFVLLCGALEKHVKCDIREDARLFYRTKVKDLVARIHGKWQEIIQNCRPTQGQLHDFWVPDS slf1_human SMC5-SMC6 complex localization factor protein 1 SLF1 ANKRD32 Ankyrin repeat domain-containing protein 32 BRCT domain-containing protein 1 BRCTD1 Smc5/6 localization factor 1 protein protein human Homo sapiens Human MEDGTPKHIIQMTGFKMEEKEALVKLLLKLDCTFIKSEKYKNCTHLIAERLCKSEKFLAACAAGKWILTKDYIIHSAKSGRWLDETTYEWGYKIEKDSRYSPQMQSAPKRWREELKRTGAPGAFHRWKVVLLVRTDKRSDSLIRVLEAGKANVILPKSSPSGITHVIASNARIKAEKEKDNFKAPFYPIQYLGDFLLEKEIQNDEDSQTNSVWTEHSNEETNKDFRKDAGFLEMKGALRETMYRTQKEMQNHEDVNVGSILIQHHKKEKFSGSSKDLKFVKMRNTFGSHTYENQKEIKKKDEDIQRSYTLRRKRKKGKESNCKKGVEHEKIKSTLRRHIYNRDQKEMKNSIFAEYAKESKAMAIKTDVDVVEIKNTLRKHIYRAQAVRYNCIRIDKQPVYNVEVKNAEFPRGVLNLIESLIEGHFFKEAIEELSTLQAHYIPPVCVLHALLENVLQDNIDTFSGRYFHILSALLHLHPPWKSPAMSRYYLELFQCPTCMKGAWSLVEVLIRSCLFNESFCHQISENIGSKVLHLTLLKFFFNLIESEVQHLSQKLYDWSDSQNLKITGKAMLLEIFWSGSETSGLLTKPVNMLLEWTIYSHKEKFKSNDVFKHELAYLLAGILGAAIDYWIFLGLKMGRNVMRHMSDDLGSYVSLSCDDFSSQELEIFICSFSSSWLQMFVAEAVFKKLCLQSSGSVSSEPLSLQKMVYSYLPALGKTGVLGSGKIQVSKKIGQRPCFDSQRTLLMLNGTKQKQVEGLPELLDLNLAKCSSSLKKLKKKSEGELSCSKENCPSVVKKMNFHKTNLKGETALHRACINNQVEKLILLLSLPGIDINVKDNAGWTPLHEACNYGNTVCVQEILQRCPEVDLLTQVDGVTPLHDALSNGHVEIGKLLLQHGGPVLLQQRNAKGELPLDYVVSPQIKEELFAITKIEDTVENFHAQAEKHFHYQQLEFGSFLLSRMLLNFCSIFDLSSEFILASKGLTHLNELLMACKSHKETTSVHTDWLLDLYAGNIKTLQKLPHILKELPENLKVCPGVHTEALMITLEMMCRSVMEFS smc6_human Structural maintenance of chromosomes protein 6 SMC6 SMC6L1 protein protein human Homo sapiens Human MAKRKEENFSSPKNAKRPRQEELEDFDKDGDEDECKGTTLTAAEVGIIESIHLKNFMCHSMLGPFKFGSNVNFVVGNNGSGKSAVLTALIVGLGGRAVATNRGSSLKGFVKDGQNSADISITLRNRGDDAFKASVYGNSILIQQHISIDGSRSYKLKSATGSVVSTRKEELIAILDHFNIQVDNPVSVLTQEMSKQFLQSKNEGDKYKFFMKATQLEQMKEDYSYIMETKERTKEQIHQGEERLTELKRQCVEKEERFQSIAGLSTMKTNLESLKHEMAWAVVNEIEKQLNAIRDNIKIGEDRAARLDRKMEEQQVRLNEAEQKYKDIQDKLEKISEETNARAPECMALKADVVAKKRAYNEAEVLYNRSLNEYKALKKDDEQLCKRIEELKKSTDQSLEPERLERQKKISWLKERVKAFQNQENSVNQEIEQFQQAIEKDKEEHGKIKREELDVKHALSYNQRQLKELKDSKTDRLKRFGPNVPALLEAIDDAYRQGHFTYKPVGPLGACIHLRDPELALAIESCLKGLLQAYCCHNHADERVLQALMKRFYLPGTSRPPIIVSEFRNEIYDVRHRAAYHPDFPTVLTALEIDNAVVANSLIDMRGIETVLLIKNNSVARAVMQSQKPPKNCREAFTADGDQVFAGRYYSSENTRPKFLSRDVDSEISDLENEVENKTAQILNLQQHLSALEKDIKHNEELLKRCQLHYKELKMKIRKNISEIRELENIEEHQSVDIATLEDEAQENKSKMKMVEEHMEQQKENMEHLKSLKIEAENKYDAIKFKINQLSELADPLKDELNLADSEVDNQKRGKRHYEEKQKEHLDTLNKKKRELDMKEKELEEKMSQARQICPERIEVEKSASILDKEINRLRQKIQAEHASHGDREEIMRQYQEARETYLDLDSKVRTLKKFIKLLGEIMEHRFKTYQQFRRCLTLRCKLYFDNLLSQRAYCGKMNFDHKNETLSISVQPGEGNKAAFNDMRALSGGERSFSTVCFILSLWSIAESPFRCLDEFDVYMDMVNRRIAMDLILKMADSQRFRQFILLTPQSMSSLPSSKLIRILRMSDPERGQTTLPFRPVTQEEDDDQR nse3_human Non-structural maintenance of chromosomes element 3 homolog HCA4 Hepatocellular carcinoma-associated protein 4 MAGE-G1 antigen MAGEG1 Melanoma-associated antigen G1 NDNL2 Necdin-like protein 2 NSMCE3 protein protein human Homo sapiens Human MLQKPRNRGRSGGQAERDRDWSHSGNPGASRAGEDARVLRDGFAEEAPSTSRGPGGSQGSQGPSPQGARRAQAAPAVGPRSQKQLELKVSELVQFLLIKDQKKIPIKRADILKHVIGDYKDIFPDLFKRAAERLQYVFGYKLVELEPKSNTYILINTLEPVEEDAEMRGDQGTPTTGLLMIVLGLIFMKGNTIKETEAWDFLRRLGVYPTKKHLIFGDPKKLITEDFVRQRYLEYRRIPHTDPVDYEFQWGPRTNLETSKMKVLKFVAKVHNQDPKDWPAQYCEALADEENRARPQPSGPAPSS nse1_human Non-structural maintenance of chromosomes element 1 homolog NSMCE1 HSPC333 HSPC337 protein protein human Homo sapiens Human MQGSTRRMGVMTDVHRRFLQLLMTHGVLEEWDVKRLQTHCYKVHDRNATVDKLEDFINNINSVLESLYIEIKRGVTEDDGRPIYALVNLATTSISKMATDFAENELDLFRKALELIIDSETGFASSTNILNLVDQLKGKKMRKKEAEQVLQKFVQNKWLIEKEGEFTLHGRAILEMEQYIRETYPDAVKICNICHSLLIQGQSCETCGIRMHLPCVAKYFQSNAEPRCPHCNDYWPHEIPKVFDPEKERESGVLKSNKKSLRSRQH eid3-smc5-1 4058672 N/A 4058677 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false N/A 4058678 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Fig. 5A nsmce2-smc5-1 4058672 N/A 4058677 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4058679 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed physical association physical association false false false Fig. 5A slf2-smc5-1 4058672 N/A 4058677 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4058680 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5A, Supp Fig. 4D slf2-slf1-1 4058672 N/A 4058681 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4058680 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5B slf2-smc6-1 4058672 N/A 4058682 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4058680 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5D slf2-smc6-2 4058672 N/A 4058682 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4058680 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 5D nsmce1-nsmce3-3 4058673 N/A 4058683 unspecified role unspecified role neutral component neutral component purified purified lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-221 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-99 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-221 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-262 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false N/A 4058684 unspecified role unspecified role neutral component neutral component purified purified lys-144 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-258 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-139 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-139 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-144 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-258 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false 4058730 4058718 4058731 4058719 4058732 4058720 4058733 4058727 4058734 4058721 4058735 4058722 4058736 4058723 4058737 4058724 4058738 4058725 4058739 4058726 4058740 4058717 4058741 4058728 direct interaction direct interaction false false false Table 1 nsmce1-nsmce3-4 4058673 N/A 4058678 unspecified role unspecified role neutral component neutral component purified purified lys-183 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-191 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-190 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-183 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-156 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-156 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-156 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-183 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-191 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-190 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-194 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-207 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-207 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-207 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-103 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-103 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 4058683 unspecified role unspecified role neutral component neutral component purified purified lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-221 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-99 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-221 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-265 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-102 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-102 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false thr-210 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-221 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-88 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-99 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-221 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-265 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-88 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-120 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-212 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-269 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-194 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false N/A 4058684 unspecified role unspecified role neutral component neutral component purified purified lys-144 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-139 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-139 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-144 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-258 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-143 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-152 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-152 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-157 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-144 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-157 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-258 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-144 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-152 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-152 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-157 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-255 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-259 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false thr-99 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-140 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false 4058772 4058759 4058779 4058744 4058780 4058745 4058781 4058746 4058782 4058747 4058787 4058753 4058788 4058752 4058789 4058754 4058790 4058755 4058791 4058756 4058794 4058757 4058795 4058758 4058797 4058778 4058798 4058763 4058799 4058770 4058800 4058762 4058801 4058765 4058802 4058766 4058803 4058776 4058804 4058764 4058805 4058773 4058806 4058771 4058807 4058786 4058808 4058767 4058809 4058774 4058810 4058775 4058811 4058768 4058812 4058769 4058813 4058777 4058814 4058783 4058815 4058784 4058816 4058785 4058817 4058793 4058818 4058748 4058819 4058749 4058820 4058750 4058821 4058751 4058822 4058792 physical association physical association false false false Table 2 smc5-smc6-7 4058673 N/A 4058682 unspecified role unspecified role neutral component neutral component purified purified lys-206 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-206 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-628 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-628 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false thr-229 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false thr-233 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-234 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-248 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-248 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-913 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-914 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-310 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false tyr-325 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-331 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-350 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-350 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-379 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-386 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-386 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-751 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-751 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-751 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false tyr-207 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-550 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-550 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-550 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 4058677 unspecified role unspecified role neutral component neutral component purified purified lys-189 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ser-191 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-498 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-542 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-548 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-247 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-300 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-332 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-332 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-339 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-746 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ser-717 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ser-717 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-719 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-378 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-710 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-714 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false tyr-499 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-189 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-495 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 4058852 4058825 4058854 4058826 4058855 4058850 4058856 4058827 4058857 4058828 4058858 4058829 4058859 4058830 4058860 4058831 4058861 4058832 4058862 4058833 4058863 4058834 4058864 4058835 4058865 4058836 4058866 4058837 4058867 4058838 4058868 4058839 4058869 4058840 4058870 4058841 4058871 4058842 4058872 4058843 4058873 4058844 4058874 4058845 4058875 4058846 4058876 4058848 4058877 4058847 4058878 4058849 direct interaction direct interaction false false false Tables 3, 4, 5 eid3-nsmce1-2 4058673 N/A 4058682 unspecified role unspecified role neutral component neutral component purified purified lys-107 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-107 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-107 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-111 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-155 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-107 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 4058677 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false lys-192 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 4058683 unspecified role unspecified role neutral component neutral component purified purified lys-83 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 4058684 unspecified role unspecified role neutral component neutral component purified purified lys-247 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-255 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-247 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-255 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 4058678 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false lys-183 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-152 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false lys-156 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 4058892 4058889 4058894 4058882 4058895 4058883 4058896 4058884 4058897 4058885 4058900 4058891 4058901 4058881 4058902 4058886 physical association physical association false false false Table 3A slf2-smc5-2 4058674 N/A 4058680 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 4058677 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 5C smc5-slf2-1 4058674 N/A 4058680 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 4058682 unspecified role unspecified role bait bait purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 5F eid3-nsmce1-1 4058675 N/A 4058682 unspecified role unspecified role neutral component neutral component purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4058677 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4058678 unspecified role unspecified role neutral component neutral component purified purified region strep tag strep tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4058683 unspecified role unspecified role neutral component neutral component purified purified N/A 4058684 unspecified role unspecified role neutral component neutral component purified purified physical association physical association false false false Fig. 4 smc6-slf2-1 4058676 N/A 4058680 unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false region radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro N/A 4058682 unspecified role unspecified role bait bait purified purified region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 5E