DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ shihoya-2019-1 ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Shihoya W., Inoue K., Singh M., Konno M., Hososhima S., Yamashita K., Ikeda K., Higuchi A., Izume T., Okazaki S., Hashimoto M., Mizutori R., Tomida S., Yamauchi Y., Abe-Yoshizumi R., Katayama K., Tsunoda SP., Shibata M., Furutani Y., Pushkarev A., Béjà O., Uchihashi T., Kandori H., Nureki O. kandori@nitech.ac.jp; nureki@bs.s.u-tokyo.ac.jp Nature 2019 Only protein-protein interactions imex curation imex curation shihoya-2019-2 ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column experimental particp experimental participant identification experimental particp Shihoya W., Inoue K., Singh M., Konno M., Hososhima S., Yamashita K., Ikeda K., Higuchi A., Izume T., Okazaki S., Hashimoto M., Mizutori R., Tomida S., Yamauchi Y., Abe-Yoshizumi R., Katayama K., Tsunoda SP., Shibata M., Furutani Y., Pushkarev A., Béjà O., Uchihashi T., Kandori H., Nureki O. kandori@nitech.ac.jp; nureki@bs.s.u-tokyo.ac.jp Nature 2019 Only protein-protein interactions imex curation imex curation shihoya-2019-3 ecoli Escherichia coli (strain K12) light scattering light scattering experimental particp experimental participant identification experimental particp Shihoya W., Inoue K., Singh M., Konno M., Hososhima S., Yamashita K., Ikeda K., Higuchi A., Izume T., Okazaki S., Hashimoto M., Mizutori R., Tomida S., Yamauchi Y., Abe-Yoshizumi R., Katayama K., Tsunoda SP., Shibata M., Furutani Y., Pushkarev A., Béjà O., Uchihashi T., Kandori H., Nureki O. kandori@nitech.ac.jp; nureki@bs.s.u-tokyo.ac.jp Nature 2019 Only protein-protein interactions imex curation imex curation shihoya-2019-4 ecoli Escherichia coli (strain K12) atomic force microsc atomic force microscopy AFM AFM atomic force microsc experimental particp experimental participant identification experimental particp Shihoya W., Inoue K., Singh M., Konno M., Hososhima S., Yamashita K., Ikeda K., Higuchi A., Izume T., Okazaki S., Hashimoto M., Mizutori R., Tomida S., Yamauchi Y., Abe-Yoshizumi R., Katayama K., Tsunoda SP., Shibata M., Furutani Y., Pushkarev A., Béjà O., Uchihashi T., Kandori H., Nureki O. kandori@nitech.ac.jp; nureki@bs.s.u-tokyo.ac.jp Nature 2019 Only protein-protein interactions imex curation imex curation a0a151eda9_9arch AYK20_03510 protein protein Thermoplasmatales archaeon SG8-52-1 Thermoplasmatales archaeon SG8-52-1 MTENEEINFRKFRIFNGIMGVIHLIQVFLVLYLSNNFSLPITVNKPVYNEITNSISPVAETLFSIEIGPLVAMFLFISATAHILIATVLYYRYVQNLKNHMNPYRWFEYSISASFMIVIIAMLTTIYDLGTLLALFTLTAVMNLMGLMMELHNQTTQNTNWTSYIIGCIAGFVPWIVIFIPLISAESVPDFVIYIFISIAIFFNCFAINMYLQYKKIGKWKNYLHGEKVYIILSLVAKSALAWQVFAGTLRPM a0a151eda9_9eury-1 3978878 N/A 3978882 predetermined predetermined participant predetermined structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component purified purified His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Figure 1, Extended Table 1 a0a151eda9_9eury-2 3978879 N/A 3978882 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Extended Data Fig. 4 a0a151eda9_9eury-3 3978880 N/A 3978882 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Extended Data Fig. 4 a0a151eda9_9eury-4 3978881 N/A 3978882 predetermined predetermined participant predetermined unspecified role unspecified role neutral component neutral component purified purified His tag His tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 2