DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhao-2019-6 rat-brain Rattus norvegicus brain brain Brain [cerebrum] 3d-em-single electron microscopy 3D single particle reconstruction 3D-EM-single experimental particp experimental participant identification experimental particp Zhao Y., Chen S., Swensen AC., Qian WJ., Gouaux E. gouauxe@ohsu.edu Science (New York, N.Y.) 2019 Only protein-protein interactions imex curation IMEx MIMIx imex curation zhao-2019-8 rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip experimental particp experimental participant identification experimental particp Zhao Y., Chen S., Swensen AC., Qian WJ., Gouaux E. gouauxe@ohsu.edu Science (New York, N.Y.) 2019 Only protein-protein interactions imex curation imex curation ccg2_rat Voltage-dependent calcium channel gamma-2 subunit Cacng2 Neuronal voltage-gated calcium channel gamma-2 subunit Transmembrane AMPAR regulatory protein gamma-2 Stargazin Stg protein protein rat Rattus norvegicus (Rat) Rat MGLFDRGVQMLLTTVGAFAAFSLMTIAVGTDYWLYSRGVCKTKSVSENETSKKNEEVMTHSGLWRTCCLEGNFKGLCKQIDHFPEDADYEADTAEYFLRAVRASSIFPILSVILLFMGGLCIAASEFYKTRHNIILSAGIFFVSAGLSNIIGIIVYISANAGDPSKSDSKKNSYSYGWSFYFGALSFIIAEMVGVLAVHMFIDRHKQLRATARATDYLQASAITRIPSYRYRYQRRSRSSSRSTEPSHSRDASPVGVKGFNTLPSTEISMYTLSRDPLKAATTPTATYNSDRDNSFLQVHNCIQKDSKDSLHANTANRRTTPV mint p19490-2 Glutamate receptor 1 Gria1 Glur1 AMPA-selective glutamate receptor 1 GluR-A GluR-K1 Glutamate receptor ionotropic, AMPA 1 protein protein rat Rattus norvegicus (Rat) Rat MPYIFAFFCTGFLGAVVGANFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDSRDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRGPVNLAVLKLSEQGVLDKLKSKWWYDKGECGSKDSGSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGGGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL p19491-2 Glutamate receptor 2 Gria2 Glur2 AMPA-selective glutamate receptor 2 GluR-B GluR-K2 Glutamate receptor ionotropic, AMPA 2 protein protein rat Rattus norvegicus (Rat) Rat MQKIMHISVLLSPVLWGLIFGVSSNSIQIGGLFPRGADQEYSAFRVGMVQFSTSEFRLTPHIDNLEVANSFAVTNAFCSQFSRGVYAIFGFYDKKSVNTITSFCGTLHVSFITPSFPTDGTHPFVIQMRPDLKGALLSLIEYYQWDKFAYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDETYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKIQFGGANVSGFQIVDYDDSLVSKFIERWSTLEEKEYPGAHTATIKYTSALTYDAVQVMTEAFRNLRKQRIEISRRGNAGDCLANPAVPWGQGVEIERALKQVQVEGLSGNIKFDQNGKRINYTINIMELKTNGPRKIGYWSEVDKMVVTLTELPSGNDTSGLENKTVVVTTILESPYVMMKKNHEMLEGNERYEGYCVDLAAEIAKHCGFKYKLTIVGDGKYGARDADTKIWNGMVGELVYGKADIAIAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHTEEFEDGRETQSSESTNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLSKQTEIAYGTLDSGSTKEFFRRSKIAVFDKMWTYMRSAEPSVFVRTTAEGVARVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSSLGTPVNLAVLKLSEQGVLDKLKNKWWYDKGECGAKDSGSKEKTSALSLSNVAGVFYILVGGLGLAMLVALIEFCYKSRAEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI p19492-2 Glutamate receptor 3 Gria3 Glur3 AMPA-selective glutamate receptor 3 GluR-C GluR-K3 Glutamate receptor ionotropic, AMPA 3 protein protein rat Rattus norvegicus (Rat) Rat MGQSVLRAVFFLVLGLLGHSHGGFPNTISIGGLFMRNTVQEHSAFRFAVQLYNTNQNTTEKPFHLNYHVDHLDSSNSFSVTNAFCSQFSRGVYAIFGFYDQMSMNTLTSFCGALHTSFVTPSFPTDADVQFVIQMRPALKGAILSLLSYYKWEKFVYLYDTERGFSVLQAIMEAAVQNNWQVTARSVGNIKDVQEFRRIIEEMDRRQEKRYLIDCEVERINTILEQVVILGKHSRGYHYMLANLGFTDILLERVMHGGANITGFQIVNNENPMVQQFIQRWVRLDEREFPEAKNAPLKYTSALTHDAILVIAEAFRYLRRQRVDVSRRGSAGDCLANPAVPWSQGIDIERALKMVQVQGMTGNIQFDTYGRRTNYTIDVYEMKVSGSRKAGYWNEYERFVPFSDQQISNDSSSSENRTIVVTTILESPYVMYKKNHEQLEGNERYEGYCVDLAYEIAKHVRIKYKLSIVGDGKYGARDPETKIWNGMVGELVYGRADIAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHLEDNNEEPRDPQSPPDPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFRRSKIAVYEKMWSYMKSAEPSVFTKTTADGVARVRKSKGKFAFLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGVATPKGSALGTPVNLAVLKLSEQGILDKLKNKWWYDKGECGAKDSGSKDKTSALSLSNVAGVFYILVGGLGLAMMVALIEFCYKSRAESKRMKLTKNTQNFKPAPATNTQNYATYREGYNVYGTESVKI ccg8_rat Voltage-dependent calcium channel gamma-8 subunit Cacng8 Neuronal voltage-gated calcium channel gamma-8 subunit Transmembrane AMPAR regulatory protein gamma-8 protein protein rat Rattus norvegicus (Rat) Rat MESLKRWNEERGLWCEKGVQVLLTTIGAFAAFGLMTIAISTDYWLYTRALICNTTNLTAGDDGPPHRGGSGSSEKKDPGGLTHSGLWRICCLEGLKRGVCVKINHFPEDTDYDHDSAEYLLRVVRASSIFPILSAILLLLGGVCVAASRVYKSKRNIILGAGILFVAAGLSNIIGVIVYISANAGEPGPKRDEEKKNHYSYGWSFYFGGLSFILAEVIGVLAVNIYIERSREAHCQSRSDLLKAGGGAGGSGGSGPSAILRLPSYRFRYRRRSRSSSRGSSEASPSRDASPGGPGGPGFASTDISMYTLSRDPSKGSVAAGLASAGGGGGGAGVGAYGGAAGAAGGGGTGSERDRGSSAGFLTLHNAFPKEAASGVTVTVTGPPAAPAPAPPAPAAPAPGTLSKEAAASNTNTLNRKTTPV gria1-gria2-1 3978907 N/A 3978909 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 2.0 N/A 3978910 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19490-2:p.[Arg757Gly;Gly758Ala;Ser768Ala;Val772Ala;Ser778Ala] R757G/G758A/S768A/V772A/S778A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 2.0 N/A 3978911 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 2.0 direct interaction direct interaction false false false Table S1, S3, Figure 2A gria1-gria2-2 3978907 N/A 3978910 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19490-2:p.[Arg757Gly;Gly758Ala;Ser768Ala;Val772Ala;Ser778Ala] R757G/G758A/S768A/V772A/S778A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 1.0 N/A 3978911 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 3.0 direct interaction direct interaction false false false Table S1, Figure 2B gria1-gria2-3 3978907 N/A 3978909 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 2.0 N/A 3978911 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 2.0 N/A 3978912 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 2.0 direct interaction direct interaction false false false Table S1, Figure 2C, 3C, 3D gria2-gria3-5 3978907 N/A 3978911 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 3.0 N/A 3978912 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 1.0 direct interaction direct interaction false false false Table S1, S3, Figure 2D gria1-gria2-4 3978907 N/A 3978909 structure determination identification by structure determination identification by structure determination structure determination unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 2.0 N/A 3978910 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19490-2:p.[Arg757Gly;Gly758Ala;Ser768Ala;Val772Ala;Ser778Ala] R757G/G758A/S768A/V772A/S778A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 1.0 N/A 3978911 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 2.0 N/A 3978912 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 1.0 direct interaction direct interaction false false false Table S1, Figure 2E gria2-23 3978907 N/A 3978911 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rat-brain Rattus norvegicus brain brain Brain [cerebrum] Stoichiometry: 4.0 direct interaction direct interaction false false false Table S1, Figure 2F gria1-8 3978908 N/A 3978909 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 3978913 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 3978910 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19490-2:p.[Arg757Gly;Gly758Ala;Ser768Ala;Val772Ala;Ser778Ala] R757G/G758A/S768A/V772A/S778A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 3978911 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified P19491-2:p.[Thr765Ala;Pro766Ala;Ser775Ala;Val779Ala] T765A/P766A/S775A/V779A mutation mutation certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false rat-brain Rattus norvegicus brain brain Brain [cerebrum] N/A 3978912 structure determination identification by structure determination identification by structure determination structure determination antibody detection identification by antibody antibody detection unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level purified purified rat-brain Rattus norvegicus brain brain Brain [cerebrum] association association false false false Figure 2, Figure 6B