UniProt UniProt http://www.uniprot.org luu-2015-1 crigr-cho7 crigr cho7 cho7 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Luu W., Hart-Smith G., Sharpe LJ., Brown AJ. Proteins were further identified by anti-His Abs and by molecular weight. aj.brown@unsw.edu.au Journal of lipid research 2015 Only protein-protein interactions imex curation imex curation luu-2015-2 crigr-cho7 crigr cho7 cho7 anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Luu W., Hart-Smith G., Sharpe LJ., Brown AJ. Proteins were further identified by anti-His Abs and by molecular weight. aj.brown@unsw.edu.au Journal of lipid research 2015 Only protein-protein interactions imex curation imex curation dhc24_human Delta(24)-sterol reductase DHCR24 KIAA0018 24-dehydrocholesterol reductase Diminuto/dwarf1 homolog Seladin-1 3-beta-hydroxysterol Delta-24-reductase protein protein human Homo sapiens Human MEPAVSLAVCALLFLLWVRLKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKTHKNIMINLMDILEVDTKKQIVRVEPLVTMGQVTALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRCTPSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGLEAICAKFTHESQRQENHFVEGLLYSLDEAVIMTGVMTDEAEPSKLNSIGNYYKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFILPSQPGLVHPKGNEAELYIDIGAYGEPRVKHFEARSCMRQLEKFVRSVHGFQMLYADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAARH dhcr7_human 7-dehydrocholesterol reductase Sterol Delta(7)-reductase DHCR7 D7SR Delta7-sterol reductase Sterol reductase SR-2 protein protein human Homo sapiens Human MAAKSQPNIPKAKSLDGVTNDRTASQGQWGRAWEVDWFSLASVIFLLLFAPFIVYYFIMACDQYSCALTGPVVDIVTGHARLSDIWAKTPPITRKAAQLYTLWVTFQVLLYTSLPDFCHKFLPGYVGGIQEGAVTPAGVVNKYQINGLQAWLLTHLLWFANAHLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAMVKGYFFPTSARDCKFTGNFFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQRELHSHVTNAMVLVNVLQAIYVIDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPHAVGVLLLGLVGYYIFRVANHQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHSKLLVSGFWGVARHFNYVGDLMGSLAYCLACGGGHLLPYFYIIYMAILLTHRCLRDEHRCASKYGRDWERYTAAVPYRLLPGIF a0a3l7h248_crigr CgPICR_022031 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster KLILTSSASVVFEGVDIKNGTEDLPYAMKPIDYYTETKILQEREVLDANNPDKNFLTTAIRPHGIFGPRDPQLVPILIDAARKGKMKFMIGNGKNLVDFTFVENVVHGHILAAEHLSQDAALGGKAFHITNDEPIPFWTFLSRMLTGLNYEAPKYHIPYWVAYYLAFLFSLLVMVVSPLIQIQPTFTPMRVALAGTFHYYSCEKAKKLFGYRPLVTMDDAVERTVQSFHHLRKGK g3gra0_crigr 3-beta-hydroxysterol Delta (14)-reductase C-14 sterol reductase Delta(14)-sterol reductase LBR Integral nuclear envelope inner membrane protein Lamin-B receptor Sterol C14-reductase I79_000043 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MPSRKFAEGEVVRGRWPGSSLYYEVEILSYDNKSQLYTVKYKDGTELELKESDVKPLKSFKQRKGGSTSSSPSRRRSSRSRSRSRSPGRAPKGSRRSASASHQADIKEKETRREILQVKLTPLVLKPFGNSINVYNGEPEHTEKNDISRKNKQEKVILSPEGSFIATQYSLRPRREEVTVKEIESKEQKRLPKVPAPLETFQVTTPQRKSLEFGGVPGASLIMLGLPACLFLLLLQCRQKDPSLLSFPPPLPALGELWEARVCGIYLLWFFIQALFYLLPIGKVVEGTPLVDGRRLKYRLNGFNAFVLTSAIVGTSLFWGIELCYVYTHFLQFALAATVFSVVLSVYLYARSLKAPRDELSPASSGNAVYDFFIGRELNPRIGVFDLKYFCELRPGLIGWVVVNLVMLLAEMKVQERGTPSLAMVLVNSFQLLYVVDALWNEEALLTTMDIIHDGFGFMLAFGDLVWVPFTYSLQAFYLVSHPHDLSWPLASVIIALKLCGYVIFRCANSQKNAFRKNPTDPKLAHLKTIHTSTGKSLLVSGWWGFVRHPNYLGDLIMALAWSLPCGFNHLLPYFYVIYFTALLVHREARDEHQCKRKYGLAWEKYCQRVPYRILPYVY a0a3l7ipt3_crigr Squalene monooxygenase CgPICR_011593 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MWTFLGIATFTYFYKKCGDVTLANKELLLCVLVFLSLGLVLSYRCRHRNAGLLGRQQSAAGGSQFAALSDILSALPLIGFLWAKSPPESEKKEEPESSKCRKETSVSETTLTGAATSVTASSLNDPEVIIVGSGVLGSALATVLSRDGRKVTVIERDLKEPDRIVGELLQPGGYRVLKELGLGDTVEGFNAHLIHGYIVHDYESRSEVQIPYPVSENNQVQNGVAFHHGNVRVIEGVVLQLLEDGDTVTGVQYKDKETGDTKELHAPLTVVADGLFSKFRKNLISSKVSVSSHFVGFLMKNAPQFKANFAELVLLNPSPVLIYQISASETRVLVDIRGEMPRNLREYMAEQIYPQIPDHLKEAFLEASQSARLRTMPASFLPPSPVNKRGVLLLGDAYNLRHPLTGGGMTVALKDVKLWRKLLKGIPDLYDDAAVFQAKKSFYWSRKRTHSFVVNVLAQALYELFSATDDSLHQLRKACFLYFKLGGECVTGPVGLLSVLSPHPLVLIRHFFAVAIYATYFCFKSEPWATKPRALFSSGAVLYKACSIIFPLIYSEMKYLVH hmdh_crigr 3-hydroxy-3-methylglutaryl-coenzyme A reductase HMGCR protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASALAKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIADPSPQNSTTEHSKVSLGLDEDVSKRIEPSVSLWQFYLSKMISMDIEQVVTLSLAFLLAVKYIFFEQAETESTLSLKNPITSPVVTPKKAPDNCCRREPLLVRRSEKLSSVEEEPGVSQDRKVEVIKPLVVETESASRATFVLGASGTSPPVAARTQELEIELPSEPRPNEECLQILESAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSTKLPEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDGKEYQVPMATTEGCLVASTNRGCRAIGLGGGASSRVLADGMTRGPVVRLPRACDSAEVKAWLETPEGFAVIKDAFDSTSRFARLQKLHVTMAGRNLYIRFQSKTGDAMGMNMISKGTEKALLKLQEFFPEMQILAVSGNYCTDKKPAAINWIEGRGKTVVCEAVIPAKVVREVLKTTTEAMIDVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVRSHMVHNRSKINLQDLQGTCTKKSA a0a3l7i5z2_crigr CgPICR_011489 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MNLGSDPVVIVSAARTAIGGYPRRSPIRYPAGAQSSFNGALSTVPVHDLGTTVIKEVLQRAKVAPEEVSEVIFGHVLAAGCGQNPTRQASVGAGIPYPVPAWSCQMICGSGLKAVCLAAQSIAIGDSSIVVAGGMENMSKAPHLAHLRAGVKMGEMPLADSILCDGLTDAFHNYHMGITAENVAKKWQVSREAQDKVAVVSQNRAEHAQKAGHFDKEIVPVLVSSRKGPTEVKIDEFPRHGSNMEAVGKLKPYFLSDGTGTVTTANATGMNDGAAAVVLMKTTEAKRRMLKPLARIVSWSQAGVEPSIMGVGPIPAIKQAVAKAGWTLEDVDLFEINEAYAALSVAITKELGLNPEKVNIDGGAIALGHPLGASGCRILVTLLHSLERTGGTRGVAALCIGGGMGIAMCVQRG g3h0l7_crigr Squalene synthase CgPICR_007530 I79_003679 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MEFVKCLGHPEEFYNLLRFRMGGRRNFITKMDQDSLSSSLKTCYKYLNQTSRSFAAVIQALDGEIRHAVCVFYLVLRALDTVEDDMSISVEKKIPMLRNFHTFLYEPEWRFTESTQKDRQVLEDFPTISLEFRNLAEKYQTVIADICQKMGCGMAEFVDKHVTSKQDWDKYCHYVAGLVGIGLSRLFSTSEFEDPLVGEDTECANSMGLFLQKTNIIRDYLEDQQEGRAFWPQEVWGRYVKKLEDFAKPENIDVAVQCLNELITNTLHHIPHVITYLSRLRNQSVFNFCAIPQVMAIATLAACYNNQQVFKGVVKIRKGQAVTLMMDATNMPAVKAIIHQYIEEIHHRIPSSDPSSSKTKEVISAIRTQSLPNCQLISRSHYSPIYLSFVMLLAALSWQYLSTLSQVTEDYVQTGEH a0a3l7hwl4_crigr CgPICR_009611 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster DLMASKFQHNTPKTKNPNGKAESQGQWGRAWEVDWFSLASVIFLLLFAPFIVYYFIMACDQYSCSLTTPIVDIATGRASLADIWAKTPPVTAKAVQLYTLWVSFQVVLYSWLPDFCHRFLPGYVGGVQEGAVTPAGVVNKYEVNGLQAWLITHLLWFLNAYLLSWFSPTIIFDNWIPLLWCANILGYAWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMILVNVLQAIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPNAVGILLLGLVGYYIFRMANHQKDLFRRTDGRCLIWGKKPKAIECSYTSADGQKHHSKLLVSGFWGVARHLNYTGDLMGSLAYCLACGGGHLLPYFYIIYMTILLTHRCLRDEHRCANKYGRDWERYTATVPYRLLPGIF dhcr7-dhcr24-1 4063066 N/A 4063068 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4063069 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Fig. 2 dhcr24-acat2-1 4063067 N/A 4063070 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 4063071 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 4063072 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 4063073 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 4063074 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 4063075 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 4063068 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false N/A 4063076 unspecified role unspecified role prey prey cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level association association false false false Table 1