UniProt UniProt http://www.uniprot.org moroz-2009-4 in vitro In vitro ms interact detect detection by mass spectrometry ms interact detect predetermined predetermined participant predetermined Moroz OV., Burkitt W., Wittkowski H., He W., Ianoul A., Novitskaya V., Xie J., Polyakova O., Lednev IK., Shekhtman A., Derrick PJ., Bjoerk P., Foell D., Bronstein IB. Electrospray Ionisation Fourier transform ion cyclotron resonance mass spectrometry olga@ysbl.york.ac.uk BMC biochemistry 2009 Only protein-protein interactions imex curation imex curation moroz-2009-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Moroz OV., Burkitt W., Wittkowski H., He W., Ianoul A., Novitskaya V., Xie J., Polyakova O., Lednev IK., Shekhtman A., Derrick PJ., Bjoerk P., Foell D., Bronstein IB. olga@ysbl.york.ac.uk BMC biochemistry 2009 Only protein-protein interactions imex curation imex curation moroz-2009-5 in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Moroz OV., Burkitt W., Wittkowski H., He W., Ianoul A., Novitskaya V., Xie J., Polyakova O., Lednev IK., Shekhtman A., Derrick PJ., Bjoerk P., Foell D., Bronstein IB. Experiments were carried out in the presence of a fluorescent probe - 6,P-toluidinylnaphthalene-2-sulfonate (TNS). olga@ysbl.york.ac.uk BMC biochemistry 2009 Only protein-protein interactions imex curation imex curation moroz-2009-6 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Moroz OV., Burkitt W., Wittkowski H., He W., Ianoul A., Novitskaya V., Xie J., Polyakova O., Lednev IK., Shekhtman A., Derrick PJ., Bjoerk P., Foell D., Bronstein IB. olga@ysbl.york.ac.uk BMC biochemistry 2009 Only protein-protein interactions imex curation imex curation moroz-2009-1 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Moroz OV., Burkitt W., Wittkowski H., He W., Ianoul A., Novitskaya V., Xie J., Polyakova O., Lednev IK., Shekhtman A., Derrick PJ., Bjoerk P., Foell D., Bronstein IB. olga@ysbl.york.ac.uk BMC biochemistry 2009 Only protein-protein interactions imex curation imex curation moroz-2009-2 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Moroz OV., Burkitt W., Wittkowski H., He W., Ianoul A., Novitskaya V., Xie J., Polyakova O., Lednev IK., Shekhtman A., Derrick PJ., Bjoerk P., Foell D., Bronstein IB. olga@ysbl.york.ac.uk BMC biochemistry 2009 Only protein-protein interactions imex curation imex curation moroz-2009-7 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Moroz OV., Burkitt W., Wittkowski H., He W., Ianoul A., Novitskaya V., Xie J., Polyakova O., Lednev IK., Shekhtman A., Derrick PJ., Bjoerk P., Foell D., Bronstein IB. NMR chemical shift perturbation olga@ysbl.york.ac.uk BMC biochemistry 2009 Only protein-protein interactions imex curation imex curation zinc(2+) zinc(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) s10ac_human Protein S100-A12 S100A12 CGRP Calcium-binding protein in amniotic fluid 1 Calgranulin-C Extracellular newly identified RAGE-binding protein Neutrophil S100 protein S100 calcium-binding protein A12 Migration inhibitory factor-related protein 6 protein protein human Homo sapiens Human MTKLEEHLEGIVNIFHQYSVRKGHFDTLSKGELKQLLTKELANTIKNIKDKAVIDEIFQGLDANQDEQVDFQEFISLVAIALKAAHYHTHKE calcium(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) rage_human Advanced glycosylation end product-specific receptor AGER RAGE Receptor for advanced glycosylation end products protein protein human Homo sapiens Human MAAGTAVGAWVLVLSLWGAVVGAQNITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPWDSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPGKPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVSVKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRTAPIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWMKDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIEPGEEGPTAGSVGGSGLGTLALALGILGGLGTAALLIGVILWQRRQRRGEERKAPENQEEEEERAELNQSEEPEAGESSTGGP s100a12-5 4110528 N/A 4110535 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 2.0 N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 5A s100a12-6 4110528 N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 5A, 5B, 5D calcium(2+)-s100a12-1 4110528 N/A 4110537 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 1.0 N/A 4110535 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) Stoichiometry: 2.0 N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 5C, 5E, 5F Bound Zn2+ increases the affinity of the S100A12 dimers for Ca2+. s100a12-7 4110528 N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false Fig.5D calcium(2+)-s100a12-2 4110528 N/A 4110537 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110535 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false Fig. 5E, 5F Bound Zn2+ increases the affinity of the S100A12 dimers for Ca2+. calcium(2+)-s100a12-3 4110528 N/A 4110537 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110535 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 direct interaction direct interaction false false false Fig. 5E, 5F Bound Zn2+ increases the affinity of the S100A12 dimers for Ca2+. s100a12-1 4110529 N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 2.0 direct interaction direct interaction false false false Fig. 4H Dimeric in the presence of EDTA s100a12-3 4110529 N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 4.0 direct interaction direct interaction false false false Fig. 4H Tetrameric in the presence of zinc s100a12-4 4110529 N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 direct interaction direct interaction false false false Fig. 4H Hexameric in the presence of zinc and calcium s100a12-s100a12-1 4110529 N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 4H Oligomeric in the presence of increasing calcium concentrations calcium(2+)-s100a12-4 4110530 N/A 4110537 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 6B, 7A, Bound Zn2+ increases the affinity of the S100A12 dimers for Ca2+. s100a12-zinc(2+)-1 4110530 N/A 4110535 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig.6C, 7A Bound Zn2+ increases the affinity of the S100A12 dimers for Ca2+. calcium(2+)-s100a12-5 4110530 N/A 4110535 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110537 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig.7A, Bound Zn2+ increases the affinity of the S100A12 dimers for Ca2+. calcium(2+)-s100a12-6 4110531 N/A 4110537 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 direct interaction direct interaction false false false Fig. 6A, 7B Bound Zn2+ increases the affinity of the S100A12 dimers for Ca2+. s100a12-zinc(2+)-2 4110531 N/A 4110535 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 6.0 direct interaction direct interaction false false false Fig. 6A, 7B, 8 Bound Zn2+ increases the affinity of the S100A12 dimers for Ca2+. calcium(2+)-s100a12-7 4110531 N/A 4110535 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110537 unspecified role unspecified role unspecified role unspecified role N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig 6A, 7B, 8 Bound Zn2+ increases the affinity of the S100A12 dimers for Ca2+. ager-s100a12-1 4110532 N/A 4110538 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc N/A 4110536 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 2A, 2B Interaction is Ca2+ and Zn2+-dependent s100a12-ager-1 4110532 N/A 4110538 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false C-terminus fc-igg1 fc-igg1 c-term range C-terminal range c-term range c-term range C-terminal range c-term range false mouse-nso NSO-mouse myeloma subclone of NS-1 ns0 NS0 Mouse Myeloma subclone of NS-1 http://www.hpacultures.org.uk/products/celllines/generalcell/detail.jsp?refId=85110503&collection=ecacc_gc from ecacc Stoichiometry: 1.0 N/A 4110536 unspecified role unspecified role bait bait purified purified ecoli Escherichia coli (strain K12) Stoichiometry: 1.0 direct interaction direct interaction false false false 4110532 Fig. 2C Interaction is Ca2+ and Zn2+-dependent s100a12-calcium(2+)-2 4110533 N/A 4110537 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 3 Interaction is Ca2+ and Zn2+-dependent s100a12-calcium(2+)-1 4110533 N/A 4110535 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110537 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false Fig. 3 calcium(2+)-s100a12-8 4110534 N/A 4110535 unspecified role unspecified role unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4110536 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) N/A 4110537 unspecified role unspecified role unspecified role unspecified role region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction false false false Fig 9 Bound Zn2+ increases the affinity of the S100A12 dimers for Ca2+.