UniProt UniProt http://www.uniprot.org kantardzhieva-2005-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J., Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J. j.wijnholds@ioi.knaw.nl Human retina cDNA Investigative ophthalmology & visual science 2005 Only protein-protein interactions imex curation imex curation kantardzhieva-2005-5 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J., Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J. j.wijnholds@ioi.knaw.nl Human retina cDNA Investigative ophthalmology & visual science 2005 Only protein-protein interactions imex curation imex curation kantardzhieva-2005-6 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J., Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J. j.wijnholds@ioi.knaw.nl Human retina cDNA Investigative ophthalmology & visual science 2005 Only protein-protein interactions imex curation imex curation kantardzhieva-2005-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J., Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J. j.wijnholds@ioi.knaw.nl Bovine retina cDNA Investigative ophthalmology & visual science 2005 Only protein-protein interactions imex curation imex curation kantardzhieva-2005-2 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J., Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J. j.wijnholds@ioi.knaw.nl Human retina cDNA Investigative ophthalmology & visual science 2005 Only protein-protein interactions imex curation imex curation kantardzhieva-2005-4 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J., Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J. j.wijnholds@ioi.knaw.nl Human retina cDNA Investigative ophthalmology & visual science 2005 Only protein-protein interactions imex curation imex curation kantardzhieva-2005-7 human-retina Human retina cells retina retinal tissue confocal microscopy confocal microscopy immunohistochemistry immunohistochemistry Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J., Kantardzhieva A., Gosens I., Alexeeva S., Punte IM., Versteeg I., Krieger E., Neefjes-Mol CA., den Hollander AI., Letteboer SJ., Klooster J., Cremers FP., Roepman R., Wijnholds J. j.wijnholds@ioi.knaw.nl Human retina cDNA Investigative ophthalmology & visual science 2005 Only protein-protein interactions imex curation imex curation mpp4_human MAGUK p55 subfamily member 4 MPP4 ALS2CR5 DLG6 Discs large homolog 6 Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 5 protein protein protein human Homo sapiens Human MIQSDKGADPPDKKDMKLSTATNPQNGLSQILRLVLQELSLFYGRDVNGVCLLYDLLHSPWLQALLKIYDCLQEFKEKKLVPATPHAQVLSYEVVELLRETPTSPEIQELRQMLQAPHFKALLSAHDTIAQKDFEPLLPPLPDNIPESEEAMRIVCLVKNQQPLGATIKRHEMTGDILVARIIHGGLAERSGLLYAGDKLVEVNGVSVEGLDPEQVIHILAMSRGTIMFKVVPVSDPPVNSQQMVYVRAMTEYWPQEDPDIPCMDAGLPFQKGDILQIVDQNDALWWQARKISDPATCAGLVPSNHLLKRKQREFWWSQPYQPHTCLKSTLSISMEEEDDMKIDEKCVEADEETFESEELSEDKEEFVGYGQKFFIAGFRRSMRLCRRKSHLSPLHASVCCTGSCYSAVGAPYEEVVRYQRRPSDKYRLIVLMGPSGVGVNELRRQLIEFNPSHFQSAVPHTTRTKKSYEMNGREYHYVSKETFENLIYSHRMLEYGEYKGHLYGTSVDAVQTVLVEGKICVMDLEPQDIQGVRTHELKPYVIFIKPSNMRCMKQSRKNAKVITDYYVDMKFKDEDLQEMENLAQRMETQFGQFFDHVIVNDSLHDACAQLLSAIQKAQEEPQWVPATWISSDTESQ pals1_human Protein PALS1 PALS1 MAGUK p55 subfamily member 5 MPP5 Membrane protein, palmitoylated 5 Protein associated with Lin-7 1 protein protein human Homo sapiens Human MTTSHMNGHVTEESDSEVKNVDLASPEEHQKHREMAVDCPGDLGTRMMPIRRSAQLERIRQQQEDMRRRREEEGKKQELDLNSSMRLKKLAQIPPKTGIDNPMFDTEEGIVLESPHYAVKILEIEDLFSSLKHIQHTLVDSQSQEDISLLLQLVQNKDFQNAFKIHNAITVHMNKASPPFPLISNAQDLAQEVQTVLKPVHHKEGQELTALLNTPHIQALLLAHDKVAEQEMQLEPITDERVYESIGQYGGETVKIVRIEKARDIPLGATVRNEMDSVIISRIVKGGAAEKSGLLHEGDEVLEINGIEIRGKDVNEVFDLLSDMHGTLTFVLIPSQQIKPPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQQQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKEKDRFASAVPHTTRSRRDQEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPYIIFIAPPSQERLRALLAKEGKNPKPEELREIIEKTREMEQNNGHYFDTAIVNSDLDKAYQELLRLINKLDTEPQWVPSTWLR crum1_human Protein crumbs homolog 1 CRB1 protein protein human Homo sapiens Human MALKNINYLLIFYLSFSLLIYIKNSFCNKNNTRCLSNSCQNNSTCKDFSKDNDCSCSDTANNLDKDCDNMKDPCFSNPCQGSATCVNTPGERSFLCKCPPGYSGTICETTIGSCGKNSCQHGGICHQDPIYPVCICPAGYAGRFCEIDHDECASSPCQNGAVCQDGIDGYSCFCVPGYQGRHCDLEVDECASDPCKNEATCLNEIGRYTCICPHNYSGVNCELEIDECWSQPCLNGATCQDALGAYFCDCAPGFLGDHCELNTDECASQPCLHGGLCVDGENRYSCNCTGSGFTGTHCETLMPLCWSKPCHNNATCEDSVDNYTCHCWPGYTGAQCEIDLNECNSNPCQSNGECVELSSEKQYGRITGLPSSFSYHEASGYVCICQPGFTGIHCEEDVNECSSNPCQNGGTCENLPGNYTCHCPFDNLSRTFYGGRDCSDILLGCTHQQCLNNGTCIPHFQDGQHGFSCLCPSGYTGSLCEIATTLSFEGDGFLWVKSGSVTTKGSVCNIALRFQTVQPMALLLFRSNRDVFVKLELLSGYIHLSIQVNNQSKVLLFISHNTSDGEWHFVEVIFAEAVTLTLIDDSCKEKCIAKAPTPLESDQSICAFQNSFLGGLPVGMTSNGVALLNFYNMPSTPSFVGCLQDIKIDWNHITLENISSGSSLNVKAGCVRKDWCESQPCQSRGRCINLWLSYQCDCHRPYEGPNCLREYVAGRFGQDDSTGYVIFTLDESYGDTISLSMFVRTLQPSGLLLALENSTYQYIRVWLERGRLAMLTPNSPKLVVKFVLNDGNVHLISLKIKPYKIELYQSSQNLGFISASTWKIEKGDVIYIGGLPDKQETELNGGFFKGCIQDVRLNNQNLEFFPNPTNNASLNPVLVNVTQGCAGDNSCKSNPCHNGGVCHSRWDDFSCSCPALTSGKACEEVQWCGFSPCPHGAQCQPVLQGFECIANAVFNGQSGQILFRSNGNITRELTNITFGFRTRDANVIILHAEKEPEFLNISIQDSRLFFQLQSGNSFYMLSLTSLQSVNDGTWHEVTLSMTDPLSQTSRWQMEVDNETPFVTSTIATGSLNFLKDNTDIYVGDRAIDNIKGLQGCLSTIEIGGIYLSYFENVHGFINKPQEEQFLKISTNSVVTGCLQLNVCNSNPCLHGGNCEDIYSSYHCSCPLGWSGKHCELNIDECFSNPCIHGNCSDRVAAYHCTCEPGYTGVNCEVDIDNCQSHQCANGATCISHTNGYSCLCFGNFTGKFCRQSRLPSTVCGNEKTNLTCYNGGNCTEFQTELKCMCRPGFTGEWCEKDIDECASDPCVNGGLCQDLLNKFQCLCDVAFAGERCEVDLADDLISDIFTTIGSVTVALLLILLLAIVASVVTSNKRATQGTYSPSRQEKEGSRVEMWNLMPPPAMERLI a0a3q1ly00_bovin MAGUK p55 subfamily member 5 PALS1 MPP5 Protein PALS1 Protein associated with Lin-7 1 protein protein bovin Bos taurus (Bovine) Bovine MTTSHMNGHVTEESDNEVKNVDLASPEEHQKHREMAVDCPGDLGTRMMPVRRSAQLERIRQQQEDMRRRREEEGKKQELDLNSSMRLKKLAQIPPKTGIDNPIFDTEEGIVLESPHYAVKILEVEDLFASLKHIQHTLVDSQSQEDISLLLQLVQNKDFQNAFKIHNAVTVHMNKASPPFPLISNAQDLAQEVQTVLKPVHQKEGQELTALLSAPHIQALLLAHDKVAEQEMQLEPITDERVYESVGQYGGETVKIVRIEKARDIPLGATVRNEMDSVIISRIVKGGAAEKSGLLHEGDEVLEINGIEIRGKDVNEVFDLLSDMHGTLTFVLIPSQQIKPPPAKETVIHVKAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQQQREAMKQTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNCGQNELRQRLMNKEKDRFASAVPHTTRNRRDHEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSIDSVRQVINSGKICLLSLRTQSLKTLRNSDLKPYIIFIAPPSQERLRALLAKEGKNPKPEELREIIEKTREMEQNNGHYFDTAIVNSDLDKAYQELLRLINKLDTEPQWVPSTWLR pals1-mpp4-1 4063671 N/A 4063678 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4063679 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed sh3 hook region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Figs. 2A, 3A pals1-pals1-1 4063671 N/A 4063679 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4063679 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed sh3 hook region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 3A mpp4-mpp4-1 4063671 N/A 4063678 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4063678 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed sh3 hook region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false Fig. 3A mpp4-pals1-2 4063672 N/A 4063678 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 4063679 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate physical association physical association false false false Fig. 2C crb1-mpp4-1 4063672 N/A 4063680 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 4063678 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 2G mpp4-crb1-1 4063673 N/A 4063680 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 4063678 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Fig. 2F pals1-mpp4-3 4063673 N/A 4063678 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 4063679 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate physical association physical association false false false Fig. 2D crb1-pals1-1 4063674 N/A 4063681 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed N/A 4063680 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain false physical association physical association false false false mpp4-pals1-1 4063675 N/A 4063678 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4063679 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed sh3 hook region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false physical association physical association false false false pals1-mpp4-2 4063676 N/A 4063678 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 4063679 unspecified role unspecified role bait bait purified purified sh3 hook region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) physical association physical association false false false Fig. 2B crb1-pals1-2 4063677 N/A 4063679 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 4063680 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level colocalization colocalization false false false Fg. 5G