IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ argentaro-2003-1 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Argentaro A., Sim H., Kelly S., Preiss S., Clayton A., Jans DA., Harley VR. Rare diseases - Interactions investigated in the context of Rare genetic disease Sequence of protein kinase II provided by authors previously published article PMID: 8706923 with aa range 291-310 - mapped to human. vincent.harley@phimr.monash.edu.au The Journal of biological chemistry 2003 Only protein-protein interactions imex curation imex curation argentaro-2003-3 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Argentaro A., Sim H., Kelly S., Preiss S., Clayton A., Jans DA., Harley VR. Rare diseases - Interactions investigated in the context of Rare genetic disease vincent.harley@phimr.monash.edu.au The Journal of biological chemistry 2003 Only protein-protein interactions imex curation imex curation argentaro-2003-2 in vitro In vitro crosslink cross-linking study crosslink weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Argentaro A., Sim H., Kelly S., Preiss S., Clayton A., Jans DA., Harley VR. Rare diseases - Interactions investigated in the context of Rare genetic disease Cross-linker: Glutaraldehyde(CHEBI:64276) vincent.harley@phimr.monash.edu.au The Journal of biological chemistry 2003 Only protein-protein interactions imex curation imex curation argentaro-2003-4 in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Argentaro A., Sim H., Kelly S., Preiss S., Clayton A., Jans DA., Harley VR. Rare diseases - Interactions investigated in the context of Rare genetic disease acrylamide quenching of tryptophan vincent.harley@phimr.monash.edu.au The Journal of biological chemistry 2003 Only protein-protein interactions imex curation imex curation kcc2b_human Calcium/calmodulin-dependent protein kinase type II subunit beta CAMK2B CAM2 CAMKB CAMK2 protein protein human Homo sapiens Human MATTVTCTRFTDEYQLYEDIGKGAFSVVRRCVKLCTGHEYAAKIINTKKLSARDHQKLEREARICRLLKHSNIVRLHDSISEEGFHYLVFDLVTGGELFEDIVAREYYSEADASHCIQQILEAVLHCHQMGVVHRDLKPENLLLASKCKGAAVKLADFGLAIEVQGDQQAWFGFAGTPGYLSPEVLRKEAYGKPVDIWACGVILYILLVGYPPFWDEDQHKLYQQIKAGAYDFPSPEWDTVTPEAKNLINQMLTINPAKRITAHEALKHPWVCQRSTVASMMHRQETVECLKKFNARRKLKGAILTTMLATRNFSVGRQTTAPATMSTAASGTTMGLVEQAKSLLNKKADGVKPQTNSTKNSAAATSPKGTLPPAALEPQTTVIHNPVDGIKESSDSANTTIEDEDAKAPRVPDILSSVRRGSGAPEAEGPLPCPSPAPFSPLPAPSPRISDILNSVRRGSGTPEAEGPLSAGPPPCLSPALLGPLSSPSPRISDILNSVRRGSGTPEAEGPSPVGPPPCPSPTIPGPLPTPSRKQEIIKTTEQLIEAVNNGDFEAYAKICDPGLTSFEPEALGNLVEGMDFHRFYFENLLAKNSKPIHTTILNPHVHVIGEDAACIAYIRLTQYIDGQGRPRTSQSEETRVWHRRDGKWQNVHFHCSGAPVAPLQ calm_bovin Calmodulin CALM CAM protein protein bovin Bos taurus (Bovine) Bovine MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK sox9_human Transcription factor SOX-9 SOX9 protein protein human Homo sapiens Human MNLLDPFMKMTDEQEKGLSGAPSPTMSEDSAGSPCPSGSGSDTENTRPQENTFPKGEPDLKKESEEDKFPVCIREAVSQVLKGYDWTLVPMPVRVNGSSKNKPHVKRPMNAFMVWAQAARRKLADQYPHLHNAELSKTLGKLWRLLNESEKRPFVEEAERLRVQHKKDHPDYKYQPRRRKSVKNGQAEAEEATEQTHISPNAIFKALQADSPHSSSGMSEVHSPGEHSGQSQGPPTPPTTPKTDVQPGKADLKREGRPLPEGGRQPPIDFRDVDIGELSSDVISNIETFDVNEFDQYLPPNGHPGVPATHGQVTYTGSYGISSTAATPASAGHVWMSKQQAPPPPPQQPPQAPPAPQAPPQPQAAPPQQPAAPPQQPQAHTLTTLSSEPGQSQRTHIKTEQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPPITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYMNPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYTQLTRP calmidazolium calmidazolium R24571 1-[bis(4-chlorophenyl)methyl]-3-{2-[(2,4-dichlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl}-1H-imidazol-3-ium small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) sox9 dna binding region sox9 dna binding region ds dna double stranded deoxyribonucleic acid ds DNA ds dna chemical synthesis Chemical synthesis (Chemical synthesis) GGGTTAACAGAACAATGGAATCTGGTAGA calm3-camk2b-1 4075626 N/A 4075630 competitor competitor competitor neutral component neutral component binding region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false N/A 4075631 unspecified role unspecified role neutral component neutral component bovin-brain Bos taurus brain brain Brain [cerebrum] N/A 4075632 unspecified role unspecified role neutral component neutral component purified purified HMG box sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2b CaCl2 (CHEBI:3312) calm3-sox9-1 4075626 N/A 4075631 unspecified role unspecified role neutral component neutral component purified purified bovin-brain Bos taurus brain brain Brain [cerebrum] N/A 4075632 unspecified role unspecified role neutral component neutral component purified purified HMG box sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 2b CaCl2 (CHEBI:3312) calm-calmidazolium-1 4075626 N/A 4075633 inhibitor inhibitor neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) N/A 4075631 unspecified role unspecified role neutral component neutral component purified purified bovin-brain Bos taurus brain brain Brain [cerebrum] N/A 4075632 unspecified role unspecified role neutral component neutral component purified purified HMG box sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 4 CaCl2 (CHEBI:3312) calm-sox9-4 4075627 N/A 4075631 unspecified role unspecified role neutral component neutral component purified purified bovin-brain Bos taurus brain brain Brain [cerebrum] N/A 4075632 unspecified role unspecified role neutral component neutral component purified purified HMG box sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) N/A 4075634 unspecified role unspecified role neutral component neutral component direct interaction direct interaction false false false 3a The addition of mutant DNA (GGGGTTAACGTCCGCGGTAATCTGGTAGA) to the CaMSOX9 complex resulted in no changes in fluorescence emission calm-sox9-5 4075627 N/A 4075631 unspecified role unspecified role neutral component neutral component purified purified bovin-brain Bos taurus brain brain Brain [cerebrum] N/A 4075632 unspecified role unspecified role neutral component neutral component purified purified HMG box sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P48436:p.Ala158Thr mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 7a, 3a CaCl2 (CHEBI:3312) calm-sox9-1 4075628 N/A 4075631 unspecified role unspecified role neutral component neutral component purified purified bovin-brain Bos taurus brain brain Brain [cerebrum] Stoichiometry: 1.0 N/A 4075632 unspecified role unspecified role neutral component neutral component in vitro translated in vitro translated protein in vitro translated radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes Stoichiometry: 1.0 physical association physical association false false false 2 C/D CaCl2 (CHEBI:3312) EGTA (CHEBI:30740) calm-sox9-3 4075629 N/A 4075631 unspecified role unspecified role neutral component neutral component purified purified bovin-brain Bos taurus brain brain Brain [cerebrum] N/A 4075632 unspecified role unspecified role neutral component neutral component purified purified HMG box sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P48436:p.Ala158Thr mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 7b-c CaCl2 (CHEBI:3312) In both WT SOX9 and A158T SOX9 proteins, all of the tryptophan fluorescence was accessible to acrylamide quenching.