DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ paggio-2019-1 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip experimental particp experimental participant identification experimental particp Paggio A., Checchetto V., Campo A., Menabò R., Di Marco G., Di Lisa F., Szabo I., Rizzuto R., De Stefani D. rosario.rizzuto@unipd.it, diego.destefani@gmail.com Nature 2019 imex curation Only protein-protein interactions imex curation paggio-2019-2 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 bn-page blue native page bn-page experimental particp experimental participant identification experimental particp Paggio A., Checchetto V., Campo A., Menabò R., Di Marco G., Di Lisa F., Szabo I., Rizzuto R., De Stefani D. rosario.rizzuto@unipd.it, diego.destefani@gmail.com Nature 2019 imex curation Only protein-protein interactions imex curation paggio-2019-3 human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip experimental particp experimental participant identification experimental particp Paggio A., Checchetto V., Campo A., Menabò R., Di Marco G., Di Lisa F., Szabo I., Rizzuto R., De Stefani D. rosario.rizzuto@unipd.it, diego.destefani@gmail.com Nature 2019 imex curation Only protein-protein interactions imex curation paggio-2019-4 mouse-liver Mus musculus liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip experimental particp experimental participant identification experimental particp Paggio A., Checchetto V., Campo A., Menabò R., Di Marco G., Di Lisa F., Szabo I., Rizzuto R., De Stefani D. rosario.rizzuto@unipd.it, diego.destefani@gmail.com Nature 2019 imex curation Only protein-protein interactions imex curation q3urs9-1 Mitochondrial potassium channel Coiled-coil domain-containing protein 51 Ccdc51 Mitok protein protein mouse Mus musculus Mouse MTGCSPVFAMQHVVGVPRILVRRTFLGTDVTMTRTLCSPGPREKRPEAAALGLFHRLPELGRTLSHTVRHQAASTAKAWWDRYEEFVGLNEVREAQGNVTEAEKVFMVARGLVREAREGLEAQQTKLKEVRDRLDRVSREDNQYLELATLEHRMLQEEKRLRIAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALLLEAQKGPASLQEAIREQASSYSLQQKDLQDLMMDLRGLVHAEQGQGSGSPTGSSTRGKDIDGLSATMKEQLRHSRQVYSCLEGLREQLDGLEKTCSQMAGVLQLAQAPAHPGTVGPVDGALPSSLLEHGSVILALSEMEQRLEAQANRNTVSSTLVTCVTFLATLPLLYMLFKTS q9nut22 Isoform 2 of ATP-binding cassette sub-family B member 8, mitochondrial ABCB8 MABC1 Short protein protein human Homo sapiens Human MLVHLFRVGIRGGPFPGRLLPPLRFQTFSAVRYSDGYRSSSLLRAVAHLRSQLWAHLPRAPLAPRWSPSAWCWVGGALLGPMVLSKHPHLCLVALCEAEEAPPASSTPHVVGSRFNWKLFWQFLHPHLLVLGVAVVLALGAALVNVQIPLLLGQLVEVVAKYTRDHVGSFMTESQNLSTHLLILYGVQGLLTFGYLVLLSHVGERMAVDMRRALFSSLLRQDITFFDANKTGQLVSRLTTDVQEFKSSFKLVISQGLRSCTQVAGCLVSLSMLSTRLTLLLMVATPALMGVGTLMGSGLRKLSRQCQEQIARAMGVADEALGNVRTVRAFAMEQREEERYGAELEACRCRAEELGRGIALFQGLSNIAFNCMVLGTLFIGGSLVAGQQLTGGDLMSFLVASQTVQRSMANLSVLFGQVVRGLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVGQSGGGKTTVASLLERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAAREANAHEFITSFPEGYNTVVGERGTTLSGGQKQRLAIARALIKQPTVLILDEATSALDAESERVVQEALDRASAGRTVLVIAHRLSTVRGAHCIVVMADGRVWEAGTHEELLKKGGLYAELIRRQALDAPRTAAPPPKKPEGPRSHQHKS mitok_human Mitochondrial potassium channel CCDC51 Coiled-coil domain-containing protein 51 MITOK protein protein human Homo sapiens Human MMGRSPGFAMQHIVGVPHVLVRRGLLGRDLFMTRTLCSPGPSQPGEKRPEEVALGLHHRLPALGRALGHSIQQRATSTAKTWWDRYEEFVGLNEVREAQGKVTEAEKVFMVARGLVREAREDLEVHQAKLKEVRDRLDRVSREDSQYLELATLEHRMLQEEKRLRTAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALLLEAQKGPVSLQEAIREQASSYSRQQRDLHNLMVDLRGLVHAAGPGQDSGSQAGSPPTRDRDVDVLSAALKEQLSHSRQVHSCLEGLREQLDGLEKTCSQMAGVVQLVKSAAHPGLVEPADGAMPSFLLEQGSMILALSDTEQRLEAQVNRNTIYSTLVTCVTFVATLPVLYMLFKAS mitos_human Mitochondrial potassium channel ATP-binding subunit Mitochondrial ATP-binding cassette 1 ABCB8 MABC1 ATP-binding cassette sub-family B member 8, mitochondrial MITOSUR Mitochondrial sulfonylurea-receptor protein protein human Homo sapiens Human MLVHLFRVGIRGGPFPGRLLPPLRFQTFSAVRNTWRNGKTGQLHKAEGEYSDGYRSSSLLRAVAHLRSQLWAHLPRAPLAPRWSPSAWCWVGGALLGPMVLSKHPHLCLVALCEAEEAPPASSTPHVVGSRFNWKLFWQFLHPHLLVLGVAVVLALGAALVNVQIPLLLGQLVEVVAKYTRDHVGSFMTESQNLSTHLLILYGVQGLLTFGYLVLLSHVGERMAVDMRRALFSSLLRQDITFFDANKTGQLVSRLTTDVQEFKSSFKLVISQGLRSCTQVAGCLVSLSMLSTRLTLLLMVATPALMGVGTLMGSGLRKLSRQCQEQIARAMGVADEALGNVRTVRAFAMEQREEERYGAELEACRCRAEELGRGIALFQGLSNIAFNCMVLGTLFIGGSLVAGQQLTGGDLMSFLVASQTVQRSMANLSVLFGQVVRGLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVGQSGGGKTTVASLLERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAAREANAHEFITSFPEGYNTVVGERGTTLSGGQKQRLAIARALIKQPTVLILDEATSALDAESERVVQEALDRASAGRTVLVIAHRLSTVRGAHCIVVMADGRVWEAGTHEELLKKGGLYAELIRRQALDAPRTAAPPPKKPEGPRSHQHKS mitok_mouse Mitochondrial potassium channel Ccdc51 Mitok Coiled-coil domain-containing protein 51 protein protein mouse Mus musculus Mouse MTGCSPVFAMQHVVGVPRILVRRTFLGTDVTMTRTLCSPGPREKRPEAAALGLFHRLPELGRTLSHTVRHQAASTAKAWWDRYEEFVGLNEVREAQGNVTEAEKVFMVARGLVREAREGLEAQQTKLKEVRDRLDRVSREDNQYLELATLEHRMLQEEKRLRIAYLRAEDSEREKFSLFSAAVRESHEKERTRAERTKNWSLIGSVLGALIGVAGSTYVNRVRLQELKALLLEAQKGPASLQEAIREQASSYSLQQKDLQDLMMDLRGLVHAEQGQGSGSPTGSSTRGKDIDGLSATMKEQLRHSRQVYSCLEGLREQLDGLEKTCSQMAGVLQLAQAPAHPGTVGPVDGALPSSLLEHGSVILALSEMEQRLEAQANRNTVSSTLVTCVTFLATLPLLYMLFKTS mitos_mouse Mitochondrial potassium channel ATP-binding subunit Abcb8 ATP-binding cassette sub-family B member 8, mitochondrial Mitochondrial sulfonylurea-receptor Mitosur protein protein mouse Mus musculus Mouse MLVHLFRFGIRGGPVPGWSLQSLRFQTFSAARSSDDRLSSHLLRTVAQLRVQLRAHLPRAPPASHWSPSAWCWVGGTLVVPAVLWQHPRLCLIALCEAKESPPAQPTRAPELRFNWKLFWHFLHPHLLALGAAIVLALGAALVNVQIPLLLGQLVEIVAKYTRDHMGSFVSESRKLSVQLLLLYGVQGLLTFGYLVLLSHIGERMAMDMRKALFSSLLRQDIAFFDAKKTGQLVSRLTTDVQEFKSSFKLVISQGLRSCTQVIGSLVSLSMLSPRLTLMLAVVTPALMGVGTLMGSGLRKLSRQCQEQIARATGVADEALGNVRTVRAFAMEKREEERYQAELESCCCKAEELGRGIALFQGLSNIAFNCMVLGTLFIGGSLVAGQQLKGGDLMSFLVASQTVQRSMASLSVLFGQVVRGLSAGARVFEYMALSPVIPLTGGYCIPNKDIRGSITFQNVTFSYPCRPGFNVLKDFTLKLPSGKIVALVGQSGGGKTTVASLLERFYDPEAGSVTLDGHDLRTLNPSWLRGQVIGFISQEPVLFATTIMENIRFGKLDASDEEVYTAAREANAHEFISSFPDGYSTVVGERGTTLSGGQKQRLAIARALIKQPTVLILDEATSALDAESERVVQEALDRASAGRTVLVIAHRLSTVRAAHSIIVMANGQVCEAGTHEELLKKGGLYSELIRRQTLDASLTSTPPAEKPEDPKSCQSKA abcb8-ccdc51-2 3727753 N/A 3727757 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey over-expressed over expressed level over-expressed V5 Tag V5 Tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 3727758 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Myc Tag Myc Tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false FLAG Tag FLAG Tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q9NUT2-2:p.Lys496Ala K513A mutation mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain false human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association false false false Fig3a abcb8-ccdc51-1 3727754 N/A 3727759 western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 3727760 western blot western blot Immuno blot Immuno blot unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association false false false Fig 3b ccdc51-abcb8-1 3727755 N/A 3727759 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 N/A 3727760 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 physical association physical association false false false Ext Data Fig 6b ccdc51-abcb8-2 3727756 N/A 3727761 western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level mouse-liver Mus musculus liver liver N/A 3727762 western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level mouse-liver Mus musculus liver liver physical association physical association false false false Fig 3c